CN205941300U - Optical system for particle analyzer - Google Patents
Optical system for particle analyzer Download PDFInfo
- Publication number
- CN205941300U CN205941300U CN201620912521.9U CN201620912521U CN205941300U CN 205941300 U CN205941300 U CN 205941300U CN 201620912521 U CN201620912521 U CN 201620912521U CN 205941300 U CN205941300 U CN 205941300U
- Authority
- CN
- China
- Prior art keywords
- laser
- optical system
- mode fiber
- particle analyzer
- hot spot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses an optical system for particle analyzer, including laser plastic illumination element, sheath flow pond and signal receiving and processing unit, laser plastic illumination element includes laser instrument, single mode fiber and laser shaping module, the laser instrument transmits the laser that sends to laser shaping module through single mode fiber, laser shaping module is used for carrying out the collimation, assembling and plastic processing the single mode fiber emitting laser, the sheath flow pond is used for test samples detection area's sample stream, the signal receiving and processing unit is used for receiving follows the useful scattered light signal that sends in the sample test region and carries out the photoelectric conversion formation signal of telecommunication. The utility model discloses a single mode fiber has avoided the direct wide -angle laser that is sent by semiconductor laser of collecting in optical system as the optical propagation means for the numerical aperture who collects lens can reduce to 0.3 or littleer, has avoided high -order transverse mode laser that the laser instrument sent to the influence of system simultaneously, makes illumination light spot pattern rule, and the density of shining is even.
Description
Technical field
This utility model is related to particle analyzer field, particularly a kind of particle analyzer optical system.
Background technology
In particle analysis field, differential blood count field, flow cytometry is one of conventional counting means.Streaming
Cell method is to allow particle to be measured to be taken by sheath fluid to wrap up in and pass through a detection zone.In this region, the illuminated light of cell illuminates, and to
Full spatial domain sends scattered light or fluorescence, collects useful optical information, enters data handling system, formed after carrying out opto-electronic conversion
Scatterplot, is analyzed to analysis object according to scatterplot.
The optical system of above-mentioned flow cytometry generally comprises lighting unit, sheath stream pool unit and signal reception processing list
Unit.After the light collimating and correcting that wherein light source (generally laser) is sent by lighting unit, becoming cross section is disciform hot spot,
Expose to sheath flow pool.Sheath flow pool provides an optical detection area, in this region, using sheath stream principle by leukocyte sample
Take and be rolled in sheath stream, make leukocyte pass through this region one by one.After being illuminated by the illuminated unit of the cell in this region, in full spatial domain
Send scattered light or fluorescence；Signal processing unit collects scattered light and fluorescence information according to design, and is converted to the signal of telecommunication.This
A little signals of telecommunication can form intuitively one dimensional histograms or two-dimentional or even three-dimensional scatterplot after screening, process, analyzing, thus
Classification and count information to leukocyte.
In prior art, as shown in figure 1, common numeration of leukocyte instrument is required for the light sending light source 11 through one
Optical system 12,13 focuses on the central area of sheath flow pool 2.To be swashed when particle to be measured passes through the central area of sheath flow pool 2
Light illuminates, and the scattered light sending and/or fluorescence are received by signal processing unit, are processed after opto-electronic conversion again.
The hot spot that wherein detection zone in sheath flow pool 2 for the lighting unit is formed is as shown in Fig. 2 be ellipse, major axis is about
For 60～240um, short axle is about 8～22um, for convenience of narration, selects a representative value, and major axis is 200um, and short axle is 10um.
In Fig. 2, circular object represents particle to be measured, when particle to be measured becomes queue to pass through irradiation area one by one, particle to be measured
Scattered light or fluorescence etc. can be sent under the irradiation of laser.
Light source 11 is generally semiconductor laser, and the light-emitting mode of semiconductor laser is as shown in figure 3, its laser that sends
The angle of divergence in the horizontal and vertical directions is different, taking the placement location of Fig. 3 as a example, laser instrument in the horizontal direction send out
Scattered angle is 5～10 degree, and the angle of divergence in vertical direction is 30～50 degree.As shown in fig. 1, the light that light source 11 sends is collimated
Lens 12 are shaped to directional light after collecting, and directional light continues to be shaped assembly 13 and is compressed shaping, forms the ellipse of detection zone
Circular light spot.
Now the problem is that, collimating lens 12 are generally non-spherical lens, and numerical aperture proper at present is left 0.6
The right side, the laser that is, this collimating lens sends to laser instrument, maximum capacity gauge is about 36 degree.In other words, with optical axis included angle
Light beam more than 36 can not be collimated lens 12 and collect, and in general, at the limiting edge of this lens capacity gauge, can go out
The existing spuious luminous point of diffraction, finally, the final illumination spot being formed in detection zone can be as shown in Figure 4.
In addition, for noise spectra of semiconductor lasers, use the laser of its basic mode TEM00 in theory, but to laser instrument
For itself, the light of high-order transverse mode is inevitably had to send, the light that is, laser instrument sends, be not in the horizontal and vertical directions
The Gaussian curve of one standard, but have certain fluctuation.The light of this higher order mode not can remove, and can aggravate after entrance system
Produced problem in above-mentioned Fig. 4.
When this problem can make each particle to be checked pass through irradiation area, before and after entering main spot, all can have
The bright weaker secondary hot spot of one energy, so in the follow-up signal of telecommunication converting, in addition to normal main pulse, also can
There is the subpulse of two associations, as shown in Figure 5.The subpulse of this association can introduce error or mistake to follow-up data processing
Sentence, thus affecting accuracy of detection.
Accordingly, it would be desirable to a kind of higher particle analyzer optical system of accuracy of detection.
Utility model content
The purpose of this utility model is to propose a kind of higher particle analyzer optical system of accuracy of detection；This system is fitted
For being analyzed to hemocyte counting.
The purpose of this utility model is achieved through the following technical solutions：
A kind of particle analyzer optical system that this utility model provides, including laser shaping lighting unit, sheath flow pool
And signal processing unit；
Described laser shaping lighting unit includes semiconductor laser, single-mode fiber and laser shaping module；
The laser sending is transferred to laser shaping module by single-mode fiber by described semiconductor laser；
Described laser shaping module is used for the laser of single-mode fiber outgoing is carried out collimating, converges and Shape correction；
Described sheath flow pool is used for detecting the sample flow of sample detection zone；
Described signal processing unit is used for receiving the useful scattered light signal sending from sample detection zone and carrying out
Opto-electronic conversion forms the signal of telecommunication.
Further, described laser shaping module includes collecting lens and cylindrical lenses；
Described collecting lens is arranged at the light-emitting window of single-mode fiber；Described cylindrical lenses are used for receiving after being arranged at collecting lens
Collect laser and by Laser Transmission to sheath flow pool.
Further, described single-mode fiber core diameter is about 2～6 microns, and numerical aperture (NA) is about 0.1～0.3.
Further, the laser in described single-mode fiber passes through tail optical fiber outgoing.
Further, described laser shaping lighting unit is ellipse in the light spot shape irradiating target area, transverse
For 60～240 microns, ellipse short shaft is 8～22 microns.
Due to employing technique scheme, this utility model has such advantages as：
The optical system of particle analyzer disclosed in this utility model adopts single-mode fiber as light communication means, makes half
What conductor laser sent is optically coupled into single-mode fiber, then when fiber exit, its illumination effect is that a dispersion angle is little
In 20 degree of ideal point light source, this makes follow-up beam processing system convergence idealize, and draws without consideration semiconductor laser
The large-numerical aperture entering, the problems such as high-order transverse mode, it is to avoid directly adopt the collecting lens to collect by quasiconductor in optical system
Wide-angle laser that laser instrument sends, so that the numerical aperture of collecting lens can be reduced to 0.3 or less, avoids simultaneously
The impact to system for the laser of the high-order transverse mode that laser instrument sends, can bright even density so that illumination spot regular shape.This light
System can also be used for cellanalyzer etc. and carries out, using fluidic cell method, the analyser that particle divides.
Other advantages of the present utility model, target and feature will be explained to a certain extent in the following description
State, and to a certain extent, will be apparent to those skilled in the art based on to investigating hereafter, or
Person can be instructed from practice of the present utility model.Target of the present utility model and other advantages can be said by following
Bright book is realizing and to obtain.
Brief description
Brief description of the present utility model is as follows.
Fig. 1 is lighting unit schematic diagram in prior art；
Fig. 2 is the illumination spot schematic diagram of detection zone；
Fig. 3 is semiconductor laser light-emitting mode schematic diagram；
Fig. 4 is detection zone reagent illumination spot schematic diagram；
Fig. 5 is signal of telecommunication pulse schematic diagram；
Fig. 6 is this utility model lighting unit schematic diagram.
In figure：Light source 11, laser shaping module 12, sheath flow pool 2, semiconductor laser 101, single-mode fiber 102, light-emitting window
103rd, collecting lens 104, cylindrical lenses 105；311 be outgoing laser beam, 312 be longitudinal mode oscillation spectrum, 313 be vertical transverse mode, 314
For horizontal transverse mode, 315 be far-field pattern, 316 be near-field pattern.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples.
Embodiment 1
As illustrated, a kind of particle analyzer optical system that the present embodiment provides, including laser shaping lighting unit,
Sheath flow pool 2 and signal processing unit；
Described laser shaping lighting unit includes semiconductor laser, single-mode fiber and laser shaping module 12；
The laser sending is transferred to laser shaping module 12 by single-mode fiber by described semiconductor laser
Described laser shaping module 12 is used for the laser of single-mode fiber outgoing is carried out collimating, converges and Shape correction；
Described sheath flow pool is used for detecting the sample flow of sample detection zone；
Described signal processing unit is used for receiving the useful scattered light signal sending from sample detection zone and carrying out
Opto-electronic conversion forms the signal of telecommunication.
Described laser shaping module includes collecting lens and cylindrical lenses；
Described collecting lens is arranged at the light-emitting window of single-mode fiber；Described cylindrical lenses are used for receiving after being arranged at collecting lens
Collect laser and by Laser Transmission to sheath flow pool.
Described single-mode fiber core diameter is about 2～6 microns, and numerical aperture (NA) is about 0.1～0.3.
Laser in described single-mode fiber passes through tail optical fiber outgoing.
Described laser shaping lighting unit irradiate target area light spot shape be ellipse, transverse be 60～
240 microns, ellipse short shaft is 8～22 microns.
Embodiment 2
The optical system of the particle analyzer that the present embodiment provides, including laser lighting unit, sheath flow pool and signal receive
Processing unit, wherein, described laser shaping lighting unit includes semiconductor laser, conduction single-mode fiber and laser shaping mould
Block, described laser shaping module carries out collimating, converges and Shape correction to the laser of fiber exit；
Described sheath stream unit provides sample detection zone, forms the sample being singly queued up passing through one by one for detection
Stream；
Described signal processing unit is used for receiving the useful scattered light signal sending from sample detection zone, carries out
Opto-electronic conversion, and the signal of telecommunication of conversion formation is amplified.
Described laser shaping lighting unit is used semiconductor laser as light source 11, transmits laser using single-mode fiber
Energy, coordinates one piece of cylindrical lens to be used for the laser of fiber exit is processed using one piece of sphere or aspherical mirror.
The laser direct-coupling of described semiconductor laser output injects single-mode fiber, is not directly collimated or whole
Shape is processed.
Described single-mode fiber core diameter is about 2～6 microns, and numerical aperture (NA) is about 0.1～0.3.
Laser in described single-mode fiber passes through tail optical fiber；Inapplicable self-focus lenses, direct outgoing.
The light beam of described single-mode fiber outgoing is collected by one piece of sphere or aspherical mirror, shaping, places thereafter one piece of post
Face mirror, for carrying out shaping to light beam.
Described laser shaping lighting unit irradiate target area light spot shape be ellipse, transverse be 60～
240 microns, ellipse short shaft is 8～22 microns.
The present embodiment provides optical fiber as the delivery means of light energy, makes what semiconductor laser sent to be optically coupled into list
Mode fiber, then when fiber exit, its illumination effect is the ideal point light source that a dispersion angle is less than 20 degree, and this makes subsequently
Beam processing system convergence idealization, without the large-numerical aperture considering that semiconductor laser introduces, the problems such as high-order transverse mode,
Effectively avoid high-order transverse mode light and association subpulse and error or erroneous judgement are introduced to follow-up data processing, thus affecting to examine
Survey the problem of precision.As shown in figure 3, Fig. 3 is semiconductor laser light-emitting mode schematic diagram；Wherein, 311 be outgoing laser beam,
312 is longitudinal mode oscillation spectrum, 313 is vertical transverse mode, 314 is horizontal transverse mode, 315 is far-field pattern, 316 is near-field pattern.
Embodiment 3
The present embodiment combines accompanying drawing 6 and further describes in detail：
As shown in fig. 6, the laser coupled that in Fig. 6, semiconductor laser 101 sends enters single-mode fiber 102, laser is from list
Project at the light-emitting window 103 of mode fiber, the light shooting angle of injection is not more than 20 degree (half-angle), can process as point source,
The light that the similar point source projecting sends is collected lens 104 and collects, and preliminary shaping, injects cylindrical lenses 105 afterwards and is entered
One step shaping, when beam propagation is to sheath flow pool 2, region to be detected in sheath flow pool 2, form illumination light as shown in Figure 2
Speckle.
Collecting lens 104 can be sphere or non-spherical lens, and its numerical aperture should be greater than the numerical aperture of single-mode fiber
Footpath.Collecting lens 104 has certain shaping capability simultaneously, and in theory, it converges to after collecting the light of fiber exit and treats
Detection zone, forms a circular illumination hot spot, the diameter of this circular illumination hot spot should with the major axis of final illumination spot or
Short axle is approximately the same.
Cylindrical lenses 105, on the basis of the preliminary shaping of collecting lens 104, above-mentioned circular illumination hot spot are compressed or draw
Stretch.Circular illumination spot diameter as collecting lens 104 is formed is identical with the major axis of final hot spot, then cylindrical lenses 105 meeting exists
This hot spot is compressed on short-axis direction so that illumination spot ovalisation.The circular illumination spot diameter being formed as collecting lens 104
Identical with the short axle of final hot spot, then cylindrical lenses 105 can stretch this circular illumination hot spot so that illumination light on long axis direction
Speckle becomes the ellipse of design.
The curved form of cylindrical lenses 105 can be circular curve or conic section, or the curve of more high order.Collect
Lens 104 and/or cylindrical lenses 105 have the function that Gaussian beam is carried out with homogenization and/or flat-topization are processed, so that shape
The hot spot becoming can be bright uniform.
Finally illustrate, above example only in order to the technical solution of the utility model to be described and unrestricted, although ginseng
According to preferred embodiment, this utility model is described in detail, it will be understood by those within the art that, can be to this
The technical scheme of utility model is modified or equivalent, the objective without deviating from the technical program and scope, and it all should
Cover in the middle of right of the present utility model.
Claims (8)
1. a kind of particle analyzer optical system it is characterised in that：Including laser shaping lighting unit, sheath flow pool and signal connect
Receive processing unit；
Described laser shaping lighting unit includes semiconductor laser, single-mode fiber and laser shaping module；
The laser sending is transferred to laser shaping module by single-mode fiber by described semiconductor laser；
Described laser shaping module is used for the laser of single-mode fiber outgoing is carried out collimating, converges and Shape correction；
Described sheath flow pool is used for detecting the sample flow of sample detection zone；
Described signal processing unit is used for receiving the useful scattered light signal sending from sample detection zone and carrying out photoelectricity
Conversion forms the signal of telecommunication.
2. particle analyzer optical system as claimed in claim 1 it is characterised in that：Described laser shaping module includes receiving
Collection lens and cylindrical lenses；
Described collecting lens is arranged at the light-emitting window of single-mode fiber；Described cylindrical lenses are used for collecting sharp after being arranged at collecting lens
Light and by Laser Transmission to sheath flow pool.
3. particle analyzer optical system as claimed in claim 1 it is characterised in that：Described single-mode fiber core diameter is about
For 2～6 microns, numerical aperture (NA) is about 0.1～0.3.
4. particle analyzer optical system as claimed in claim 1 it is characterised in that：Laser in described single-mode fiber leads to
Cross tail optical fiber outgoing.
5. particle analyzer optical system as claimed in claim 1 it is characterised in that：Described laser shaping lighting unit exists
The light spot shape irradiating target area is ellipse, and transverse is 60～240 microns, and ellipse short shaft is 8～22 microns.
6. particle analyzer optical system as claimed in claim 2 it is characterised in that：Described cylindrical lenses be used for compression from
The circular illumination hot spot of collecting lens outgoing, thus form oval hot spot；The circular illumination hot spot that described collecting lens is formed
Diameter is identical with the oval hot spot major axis that cylindrical lenses are formed.
7. particle analyzer optical system as claimed in claim 2 it is characterised in that：Described cylindrical lenses be used for stretching from
The circular illumination hot spot of collecting lens outgoing, thus form oval hot spot；The circular illumination hot spot that described collecting lens is formed
Diameter is identical with the short axle of the oval hot spot that cylindrical lenses are formed.
8. particle analyzer optical system as claimed in claim 2 it is characterised in that：The curved form of described cylindrical lenses
For circular curve, conic section or high order curve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620912521.9U CN205941300U (en) | 2016-08-18 | 2016-08-18 | Optical system for particle analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620912521.9U CN205941300U (en) | 2016-08-18 | 2016-08-18 | Optical system for particle analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205941300U true CN205941300U (en) | 2017-02-08 |
Family
ID=57948205
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620912521.9U Expired - Fee Related CN205941300U (en) | 2016-08-18 | 2016-08-18 | Optical system for particle analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205941300U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111337901A (en) * | 2020-04-03 | 2020-06-26 | 深圳煜炜光学科技有限公司 | Laser radar for remote detection and detection method thereof |
| CN112881720A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Sample analyzer, laser module |
-
2016
- 2016-08-18 CN CN201620912521.9U patent/CN205941300U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112881720A (en) * | 2019-11-30 | 2021-06-01 | 深圳市帝迈生物技术有限公司 | Sample analyzer, laser module |
| CN111337901A (en) * | 2020-04-03 | 2020-06-26 | 深圳煜炜光学科技有限公司 | Laser radar for remote detection and detection method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101153868B (en) | Stream type cell analyzer | |
| CN101000306B (en) | Cell analyser | |
| CN105319184B (en) | Flow cytometer, particle analysing device and flow cytometry | |
| EP1710558A2 (en) | Blood analyzer, sample analyzer, and flow cytometer | |
| CN106226224A (en) | A kind of particle analyzer optical system | |
| CN104075976B (en) | Blood cell analyzer and blood cell analysis method | |
| CN101498646A (en) | Forward-scattering signal inspection device and method, cell or particle analyzer | |
| EP3330367B1 (en) | Cell analysis apparatus using plurality of lasers | |
| CN104075981A (en) | Blood cell analyzer and blood cell analyzing method | |
| CN103597349A (en) | Identifying and enumerating early granulated cells (EGCs) | |
| CN102608004A (en) | Aerodynamic size spectrometer | |
| CN104075978A (en) | Particle analyzer, optical system for particle analyzer, and lens for particle analyzer | |
| CN205941300U (en) | Optical system for particle analyzer | |
| CN104568844A (en) | Urine sample analyzer and urine sample analyzing method | |
| CN103364316B (en) | Multi-refractive index particle size multi-point measurement device and method based on optical fiber connection | |
| CN211478007U (en) | Sample analyzer, optical system thereof and flow cytometer | |
| CN113196039B (en) | Sample optical detection device, sample detection method and sample analyzer | |
| CN115015178A (en) | Optical detection device and blood analyzer | |
| CN103293089A (en) | Reticulocyte analyzer | |
| CN110530782B (en) | Optical system and method for eliminating side lobe signal interference | |
| US20140256032A1 (en) | Light scattering sperm assesment device and method | |
| CN202177574U (en) | Photoelectric sensor for blood cell analysis | |
| KR20200018507A (en) | Measurement cuvettes for cell counting and / or characterization | |
| CN217359605U (en) | Optical system of blood analyzer and blood analyzer | |
| CN101819145B (en) | Full automatic five grouping blood analyser optical system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170208 Termination date: 20200818 |