CN114577706A - Optical detection device for cell analyzer and cell analyzer - Google Patents
Optical detection device for cell analyzer and cell analyzer Download PDFInfo
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- CN114577706A CN114577706A CN202210316756.1A CN202210316756A CN114577706A CN 114577706 A CN114577706 A CN 114577706A CN 202210316756 A CN202210316756 A CN 202210316756A CN 114577706 A CN114577706 A CN 114577706A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Abstract
The present invention relates to an optical detection device for a cell analyzer and a cell analyzer. The optical detection device for the cell analyzer comprises a detection body; the detection body includes: the device comprises a laser shaping assembly, a flow cell, a fluorescence collecting lens assembly and a lateral fluorescence detection assembly, wherein the laser shaping assembly comprises at least two lasers with different wavelengths, when sample particles pass through the flow cell, the sample particles can emit lateral scattered light and fluorescence to the periphery simultaneously after being irradiated by the laser with different wavelengths after being shaped, and the lateral scattered light and the fluorescence with different wavelengths are detected through the lateral fluorescence detection assembly of a corresponding channel. Through setting up more than one laser instrument, increase the inspection index for optical detection device is more reasonable, has guaranteed optical detection device's stability.
Description
Technical Field
The invention relates to the field of sample analysis equipment, in particular to an optical detection device for a cell analyzer and the cell analyzer.
Background
In flow cytometry, the optical detection module is one of the core modules. The optical detection module mainly comprises a laser, a flow cell, a fluorescence collecting lens, a multi-channel detector and other components.
The prior similar technology/product mainly has the following problems: the laser spot irradiated in the center of the flow cell needs to be shaped into an elliptical spot of about 80 micrometers multiplied by 10 micrometers, because the output of a commercial semiconductor laser is a circular spot, a common beam shaping method is to use two cylindrical mirrors with a focal length ratio of 8:1 in the X-axis direction and the Y-axis direction to respectively compress the spot sizes of the two axes to different degrees, but the method has the defects that the focal length of the cylindrical mirror in the Y-axis direction needs to be shorter in order to ensure that the length of the whole optical path is not too long, so that the cylindrical mirror in the Y-axis direction is closer to the flow cell, the adjusting space is small, the axial adjusting precision needs to be higher, and the stability of the cylindrical mirror has a larger influence on the final test result.
The present application is directed to creating a structure that systematically addresses the laser shaping deficiencies.
Disclosure of Invention
To achieve the above objects and other advantages and in accordance with the purpose of the invention, a first object of the present invention is to provide an optical detection device for a cell analyzer, comprising a detection body; the detection body comprises:
the laser shaping component is used for shaping the initial laser into expected laser;
the flow cell is used for allowing cells to be detected of the detection sample liquid to queue and pass under the wrapping of the sheath liquid;
the fluorescence collecting lens assembly is arranged in the direction perpendicular to the incident laser and is used for collecting a fluorescence signal and lateral scattered light generated by the laser irradiating the cell to be detected;
the lateral fluorescence detection assembly is used for detecting lateral scattered light and fluorescence passing through the lateral fluorescence detection assembly;
the laser shaping component comprises at least two lasers with different wavelengths, and the lasers with different wavelengths emit laser with different wavelengths which are emitted after being shaped by the laser shaping component; when sample particles pass through the flow cell, the sample particles are irradiated by the shaped laser with different wavelengths and then emit lateral scattered light and fluorescence to the periphery simultaneously, and the lateral scattered light and the fluorescence with different wavelengths are detected through the lateral fluorescence detection assemblies of the corresponding channels.
Preferably, the laser shaping assembly comprises a first laser, a second laser, a beam waist adjusting lens group, a shaping assembly reflecting mirror, a dichroic mirror and a focusing lens, wherein the laser wavelength generated by the first laser is larger than that generated by the second laser; laser generated by the first laser passes through the beam waist adjusting lens group, is reflected by the shaping component reflector, and then is combined with laser generated by the second laser into one beam after being transmitted through the dichroic mirror; the laser light of two wavelengths passes through the focusing lens to form a light spot with a desired size at the center of the flow cell.
Preferably, the beam waist adjusting lens group comprises two groups of double cemented lenses, so as to ensure that the beam waist position after laser shaping is located at the center of the flow cell by adjusting the distance between the two groups of double cemented lenses.
Preferably, the first and second liquid crystal display panels are,
the fluorescence collection lens assembly comprises a plurality of groups of lenses, and light spots of the two lasers with the intermediate wavelengths in the flow cell pass through the plurality of groups of lenses and then the distance of the light spots in the Y-axis direction is enlarged.
Preferably, the cell detecting device further comprises a forward scattering assembly, wherein the forward scattering assembly comprises an optical filter and a forward detector, and the forward detector is used for collecting forward scattering signals generated by irradiating the light source on the cell to be detected.
Preferably, the target surface of the forward detector is offset at an angle from the direction of laser incidence.
Preferably, the fluorescence collecting device further comprises a three-dimensional adjusting frame, and a locking structure is further arranged on the three-dimensional adjusting frame to lock the fluorescence collecting lens assembly at the position after the positions between the groups of lenses and the flow cell are adjusted.
Preferably, the lateral fluorescence detection assembly comprises a first lateral fluorescence detection assembly and a second lateral fluorescence detection assembly, a detection assembly reflector is arranged between the first lateral fluorescence detection assembly and the second lateral fluorescence detection assembly, and the detection assembly reflector can reflect fluorescence excited by the first laser; simultaneously transmitting the side scattered light and fluorescence excited by the second laser; the fluorescence excited by the reflected first laser, the lateral scattered light excited by the second laser and the fluorescence are collimated into parallel light by the collimating lens, then the fluorescence of different wave bands is separated by the dichroic mirror, and the parallel light is detected by the detection modules of the fluorescence wavelength channels.
Preferably, the filter models of the different fluorescence wavelength channels are different.
It is a second object of the present invention to provide a cell analyzer comprising a reaction cell, a reagent supply means, a sampling unit, a sheath fluid supply means, a transport means, an analyzing unit, an output unit, and an optical detection means for a cell analyzer as described above.
Compared with the prior art, the invention has the beneficial effects that:
the invention provides an optical detection device for a cell analyzer and the cell analyzer. The optical detection device for the cell analyzer comprises a detection body; the detection body includes: the device comprises a laser shaping component, a flow cell, a fluorescence collecting lens component and a lateral fluorescence detection component, wherein the laser shaping component comprises at least two lasers with different wavelengths, when sample particles pass through the flow cell, the sample particles can emit lateral scattered light and fluorescence to the periphery simultaneously after being irradiated by laser with different wavelengths after being shaped, and the lateral scattered light and the fluorescence with different wavelengths are detected through the lateral fluorescence detection component of a corresponding channel. Through setting up more than one laser instrument, increase the inspection index for optical detection device is more reasonable, has guaranteed optical detection device's stability.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic structural diagram of an optical inspection apparatus;
FIG. 2 is a schematic view of an optical inspection apparatus;
FIG. 3 is a schematic view of the inner lens of the fluorescence collection lens assembly.
Description of the drawings:
10. a laser shaping component; 11. a first laser; 12. a second laser; 13. a beam waist adjusting lens group; 14. a shaping component mirror; 15. a shaping component dichroic mirror; 16. a shaping component focusing lens;
20. a flow cell;
30. a fluorescence collection lens assembly;
40. a forward scattering component; 41. an optical filter; 42. a forward detector;
50. a lateral fluorescence detection assembly; 51. a first lateral fluorescence detection assembly; 52. a second lateral fluorescence detection assembly; 53. a detection assembly mirror; 54. a detection component dichroic mirror; 55. a detection assembly collimating lens; 56. a detection assembly optical filter; 57. a detection assembly focus lens; 58. and a detector.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that any combination of the embodiments or technical features described below can be used to form a new embodiment without conflict.
In the following description, suffixes such as "module", "part", or "unit" used to indicate elements are used only for facilitating the description of the present invention, and have no particular meaning in themselves. Thus, "module", "component" or "unit" may be used mixedly.
Example 1
An optical detection device for a cell analyzer, as shown in fig. 1-2, includes a detection body; the detection body includes:
a laser shaping component 10 for shaping the initial laser light into a desired laser light;
the flow cell 20, the flow cell 20 is used for the cell to be detected of the detection sample liquid to queue and pass under the wrapping of the sheath liquid;
it should be understood that when a sample is tested, the sample particles will be arranged in a column and pass through the center of the flow cell 20 one by being wrapped by the sheath fluid, and since the sample to be tested will be marked by the fluorescent probe, when the sample particles pass through the center of the flow cell 20, the sample particles will emit scattered light and fluorescence simultaneously to the periphery after being irradiated by the laser.
The fluorescence collection lens assembly 30 is arranged in a direction perpendicular to the incident laser, and the fluorescence collection lens assembly 30 is used for collecting a fluorescence signal generated by irradiating the laser on a cell to be detected;
a lateral fluorescence detection assembly 50 for detecting lateral scattered light and fluorescence passing through the lateral fluorescence detection assembly 50;
the laser shaping component comprises at least two lasers with different wavelengths, and the lasers with different wavelengths excite the lasers with different wavelengths, and the lasers are shaped by the laser shaping component 10 and then emitted; when sample particles pass through the flow cell 20, the sample particles are irradiated by the shaped laser with different wavelengths and then emit lateral scattered light and fluorescence to the periphery simultaneously, and the lateral scattered light and the fluorescence with different wavelengths are detected through the lateral fluorescence detection assemblies of the corresponding channels. The laser shaping assembly adopts more than one laser, and the inspection indexes are increased, so that the optical detection device is more reasonable, and the more the lasers are, the more testable index items are; the stability of the optical detection device is ensured.
In order to improve the stability of laser shaping, when the laser shaping assembly 10 includes two lasers, in some embodiments, as shown in fig. 1-2, the laser shaping assembly 10 includes a first laser 11, a second laser 12, a beam waist adjusting lens group 13, a shaping assembly reflector 14, a shaping assembly dichroic mirror 15, and a shaping assembly focusing lens 16 (for shaping a laser spot into an 80 μm × 10 μm elliptical spot), wherein the laser wavelength generated by the first laser 11 is larger than the laser wavelength generated by the second laser 12; laser generated by the first laser 11 passes through the beam waist adjusting lens group 13, is reflected by the shaping component reflector 14, then passes through the shaping component dichroic mirror 15 and is combined with laser generated by the second laser 12 into a beam; the two wavelengths of laser light pass through a shaping assembly focusing lens 16 to form a spot of desired size at the center of flow cell 20.
The first laser 11 is a laser with the excitation wavelength of 638nm laser, the second laser 12 is a laser with the excitation wavelength of 488nm laser, at this time, the laser emitted from the outlets of the first laser 11 and the second laser 12 is a collimated elliptical spot, and the length-width ratio of the elliptical spot is 8: 1; the laser with the wavelength of 488nm passes through the beam waist adjusting lens group 13 and then is reflected on the surface of the shaping component dichroic mirror 15; the laser with the wavelength of 638nm passes through the beam waist adjusting lens group 13, is reflected by the shaping component reflector 14, then transmits through the shaping component dichroic mirror 15 and is combined with the laser with the wavelength of 488nm into a beam; the laser with two wavelengths is finally converged into an elliptical spot of about 80 μm × 10 μm at the center of the flow cell 20 by the focusing lens 16 of the shaping component to complete the shaping of the laser.
In order to form a light spot with a desired size, the light path of the 488nm laser is that the second laser 12 emits light, then the light passes through the beam waist adjusting lens group 13, then is reflected at the dichroic mirror 15, and finally is focused at the center of the flow cell 20 after passing through the focusing lens 16; the optical path of the laser with the wavelength of 638nm is that the first laser 11 is emitted, then passes through the beam waist adjusting lens group 13, then is reflected by the reflecting mirror 14, then is transmitted by the dichroic mirror 15, and finally is focused at the center of the flow cell 20 through the focusing lens 16.
It should be understood that the laser emitted from the laser is an elliptical spot after being shaped to a certain extent, and the parallel light is emitted from the laser to the front of the focusing lens, so the positions of the beam waist adjusting lens group, the dichroic mirror and the reflecting mirror can be any position on the parallel light path, but the centers of the lenses are coaxial with the laser, and finally the center of the flow cell is located at the focal plane of the focusing lens.
It will be appreciated that the focusing lens is axially adjustable, acting to adjust the location of the spot of minimum focus axially to the centre of the flow cell; the reflector and the dichroic mirror can adjust the pitch and yaw angles, and the pitch angle adjustment has two functions, namely, the distance between light spots of 488nm and 638nm in the center of the flow cell is adjusted to a fixed value (such as 250 mu m) in the height direction, so that fluorescence excited by two lasers can be separated by the fluorescence collecting lens group, and no fluorescence crosstalk is generated, and the absolute position of the two lasers in the center height direction of the flow cell is adjusted to a proper position, so that the two lasers can be matched with a light splitting box behind; the deflection angle of the reflecting mirror and the dichroic mirror is used for adjusting two laser spots to the center of the flow cell along the horizontal direction (left and right); the beam waist adjusting lens group is used for adjusting the beam waist position of one laser to be overlapped with the beam waist position of the other laser as much as possible when the beam waist positions (along the axial direction) of the two lasers are different greatly, so that the beam waist positions of the two lasers can be adjusted to the center of the flow cell at the same time (the beam waist position is the position where a light spot is focused to the minimum).
The shaping component focusing lens 16 can be adjusted back and forth along the axial direction, and the front and back adjustment of the shaping component focusing lens 16 is not very sensitive to the position of the light spot in the center of the flow cell 20 due to the long focal length of the shaping component focusing lens 16, so that the required adjustment precision is not high, and the shaping component focusing lens can be locked by a set screw after the adjustment is finished.
The shaping assembly reflector 14 and the shaping assembly dichroic mirror 15 are arranged on a two-dimensional adjusting frame, the pitch angle and the yaw angle can be adjusted respectively, and the shaping assembly reflector and the shaping assembly dichroic mirror can be locked after the adjustment is finished; the position of the 488nm wavelength light spot and the 638nm wavelength light spot at the center of the flow cell 20 are the same along the X direction (realized by adjusting the deflection angle of the shaping assembly reflector 14 and the shaping assembly dichroic mirror 15), are separated up and down along the Y direction and keep a certain distance, the position of the 638nm wavelength laser light is below, and the position of the 488nm wavelength laser light is above (realized by adjusting the pitch angle of the shaping assembly reflector 14 and the shaping assembly dichroic mirror 15).
The beam waist adjusting lens group 13 comprises two groups of double cemented lenses, and a lens adjusting structure is installed on the detection body to adjust the distance between the two groups of double cemented lenses through the lens adjusting structure so as to ensure that the beam waist position after laser shaping is located at the center of the flow cell. In some embodiments, the lens adjustment structure further comprises a locking structure thereon to lock the position of the two groups of double cemented lenses after the lens adjustment structure has been adjusted.
To improve the efficiency of fluorescence collection, in some embodiments, the fluorescence collection lens assembly 30 includes several sets of lenses through which the spots of the two medium wavelength laser light in the flow cell 20 pass to increase their distance in the Y-axis direction by a factor of about 15. Specifically, the flow cell 20 is laterally fitted with a fluorescence collection lens assembly 30 in a direction perpendicular to the incident laser light, and in some embodiments, as shown in FIGS. 1-3, the fluorescence collection lens assembly 30 comprises 6 sets of lenses with NA up to 0.95, and the fluorescence collection efficiency is doubled over the NA of a conventional aspherical mirror, which results in better signal-to-noise ratio.
It will be appreciated that the efficiency of fluorescence collection is doubled over conventional aspherical mirrors, and not that of NA, meaning that more fluorescence signal is collected and thus the signal-to-noise ratio is also improved.
The fluorescence collecting lens assembly 30 has a high amplification factor, light spots of two kinds of wavelength lasers at the center of the flow cell 20 are separated by hundreds of micrometers along the Y axis, after amplification, the side scattering light of the two kinds of wavelength lasers and the light spots of fluorescence focused by the fluorescence collecting lens assembly 30 are separated by several millimeters, and the phenomenon of fluorescence crosstalk cannot be generated. Meanwhile, the fluorescence collecting lens assembly 30 adopts an image space telecentric light path design, so that the side scattered light and the fluorescence of the laser with two wavelengths can be focused into a very small light spot, and the image height is almost kept unchanged along with the change of the propagation distance, thereby avoiding the possibility of fluorescence crosstalk.
In some embodiments, a three-dimensional adjusting frame is further included, and a locking structure is further provided on the three-dimensional adjusting frame to lock the fluorescence collection lens assembly 30 in the adjusted position after adjusting the positions between the sets of lenses and the flow cell 20.
In some embodiments, the flow cell 20 further includes a forward scattering component 40 after passing through the flow cell 20 along the direction of incidence of the laser light, the forward scattering component 40 includes a filter 41 and a forward detector 42, in some embodiments, the filter 41 is a narrow-band filter capable of passing through the 488nm wavelength laser light, only the scattered light of the 488nm wavelength laser light passes through and then enters the forward detector 42, and the forward detector 42 is used for collecting a forward scattering signal generated by the light source irradiating on the cell to be detected; the forward scatter signal (also referred to as low angle scatter signal) collected by the forward scatter assembly 40 can be indicative of the size of the cell volume being measured.
In some embodiments, the target surface of the forward detector 42 is offset from the direction of laser incidence by an angle to avoid that the laser-transmissive portion also enters the target surface of the forward detector 42.
When the lasers are lasers with two different wavelengths, the lateral fluorescence detection assembly 50 includes a first lateral fluorescence detection assembly 51 and a second lateral fluorescence detection assembly 52, a detection assembly reflector 53 is disposed between the first lateral fluorescence detection assembly 51 and the second lateral fluorescence detection assembly 52, and the detection assembly reflector 53 can reflect fluorescence excited by the first laser 11; simultaneously, the side scattered light and fluorescence excited by the second laser 12 are transmitted; the reflected fluorescence excited by the first laser 11, the side scattered light excited by the second laser 12, and the fluorescence are collimated into parallel light by the collimating lens 55 of the detection assembly, and then the fluorescence of different wavelength bands is separated by the dichroic mirror 54 of the detection assembly, and then is sequentially detected by the detection modules of the respective fluorescence wavelength channels. Specifically, the side scattered light and fluorescence excited by 638nm wavelength and 488nm wavelength pass through the fluorescence collecting lens assembly 30 and enter the first side fluorescence detecting assembly 51 and the first side fluorescence detecting assembly 52 with 638nm wavelength respectively to be detected.
The detection modules may be provided in multiple sets, and in some embodiments, the detection modules include seven sets of detection modules (six sets of which are side fluorescence and one set of which is side scattered light), each detection module includes a detection module filter 56, a detection module focusing lens 57 and a detector 58, wherein the type parameters of the detection module filters 56 of each detection module are different, and the types of the detection module focusing lens 57 and the detector 58 are the same.
Specifically, the detection assembly dichroic mirror 54 may be used to separate the side-scattered light from the different wavelength bands of fluorescence; in some embodiments, the detection assembly dichroic mirror 54 has a total of five plates, all of which have different model parameters.
In the second side fluorescent light detecting component 52 with wavelength of 488nm, because the side scattered light and the fluorescent light excited by wavelength of 488nm do not directly transmit through the reflector 53, they are collimated into parallel light by the collimating lens 55 of the detecting component, and the side scattered light and the fluorescent light with different wave bands are separated by the dichroic mirror 54 of three different detecting components and enter into the respective channels for detection, the structure of each channel is composed of the light filter 56 of the detecting component, the focusing lens 57 of the detecting component and the detector 58, the types of the light filters 56 of the detecting components of different channels are different, and the rest components are the same.
Example 2
A cell analyzer comprising a reaction cell, a reagent supply device, a sampling unit, a sheath fluid supply device, a transport device, an analyzing unit, an output unit, and the optical detection device for cell analyzer as in embodiment 1.
An optical detection device for a cell analyzer includes a detection body; the detection body includes:
a laser shaping component 10 for shaping the initial laser light into a desired laser light;
the flow cell 20, the flow cell 20 is used for the cell to be detected of the detection sample liquid to queue and pass under the wrapping of the sheath liquid;
the fluorescence collection lens assembly 30, the fluorescence collection lens assembly 30 is arranged in the direction perpendicular to the incident laser, and the fluorescence collection lens assembly 30 is used for collecting the fluorescence signal generated by the laser irradiating the cell to be detected;
a lateral fluorescence detection assembly 50 for detecting lateral scattered light and fluorescence passing through the lateral fluorescence detection assembly 50;
the laser shaping component comprises at least two lasers with different wavelengths, and the lasers with different wavelengths excite the lasers with different wavelengths, and the lasers are shaped by the laser shaping component 10 and then emitted; when sample particles pass through the flow cell 20, the sample particles are irradiated by the shaped laser light with different wavelengths and then emit lateral scattered light and fluorescence to the periphery simultaneously, and the lateral scattered light and the fluorescence with different wavelengths are detected through the lateral fluorescence detection assemblies of the corresponding channels. The laser shaping assembly provided by the invention adopts more than one laser, so that the optical detection device is more reasonable, and the stability of the optical detection device is ensured.
The embodiments in the present specification are described in a progressive manner, and the same and similar parts among the embodiments are referred to each other, and each embodiment focuses on the differences from the other embodiments. In particular, for the system embodiment, since it is substantially similar to the method embodiment, the description is simple, and for the relevant points, reference may be made to the partial description of the method embodiment.
The foregoing is illustrative of embodiments of the present disclosure and is not intended to limit one or more embodiments of the present disclosure. Various modifications and alterations to one or more embodiments described herein will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of one or more embodiments of the present specification should be included in the scope of claims of one or more embodiments of the present specification.
Claims (10)
1. An optical detection device for a cell analyzer includes a detection body; characterized in that, the detection body includes:
the laser shaping component is used for shaping the initial laser into expected laser;
the flow cell is used for allowing cells to be detected of the detection sample liquid to queue and pass under the wrapping of the sheath liquid;
the fluorescence collecting lens assembly is arranged in the direction perpendicular to the incident laser and is used for collecting a fluorescence signal and lateral scattered light generated by the laser irradiating the cell to be detected;
the lateral fluorescence detection assembly is used for detecting lateral scattered light and fluorescence passing through the lateral fluorescence detection assembly;
the laser shaping component comprises at least two lasers with different wavelengths, and the lasers with different wavelengths emit laser with different wavelengths which are emitted after being shaped by the laser shaping component; when sample particles pass through the flow cell, the sample particles are irradiated by the shaped laser with different wavelengths and then emit lateral scattered light and fluorescence to the periphery simultaneously, and the lateral scattered light and the fluorescence with different wavelengths are detected through the lateral fluorescence detection assemblies of the corresponding channels.
2. The optical detection device for cell analyzer according to claim 1, wherein the laser shaping assembly comprises a first laser, a second laser, a beam waist adjusting lens group, a shaping assembly reflecting mirror, a dichroic mirror, and a focusing lens, wherein the laser generated by the first laser has a wavelength of laser larger than that generated by the second laser; laser generated by the first laser passes through the beam waist adjusting lens group, is reflected by the shaping component reflector, and then is combined with laser generated by the second laser into one beam after being transmitted through the dichroic mirror; laser light of two wavelengths passes through the focusing lens to form a spot of a desired size at the center of the flow cell.
3. The optical detection device for a cell analyzer as claimed in claim 2, wherein the beam waist adjusting lens group comprises two sets of double cemented lenses to ensure that the beam waist position after laser shaping is located at the center of the flow cell by adjusting the distance between the two sets of double cemented lenses.
4. The optical detection device for cell analyzer according to claim 1,
the fluorescence collection lens assembly comprises a plurality of groups of lenses, and light spots of the two lasers with the intermediate wavelengths in the flow cell pass through the plurality of groups of lenses and then the distance of the light spots in the Y-axis direction is enlarged.
5. The optical detection device for cell analyzer according to claim 1, further comprising a forward scattering component, wherein the forward scattering component comprises an optical filter and a forward detector, and the forward detector is used for collecting a forward scattering signal generated by the light source irradiating the cell to be detected.
6. The optical detection device for cell analyzer according to claim 5, wherein the target surface of the forward probe is deviated from the laser incidence direction by an angle.
7. The optical detection device for a cell analyzer of claim 1, further comprising a three-dimensional adjustment frame, wherein the three-dimensional adjustment frame further comprises a locking structure for locking the fluorescence collection lens assembly in position after adjusting the position between the sets of lenses and the flow cell.
8. The optical detection device for cell analyzer according to claim 1, wherein the lateral fluorescence detection assembly comprises a first lateral fluorescence detection assembly and a second lateral fluorescence detection assembly, a detection assembly reflector is disposed between the first lateral fluorescence detection assembly and the second lateral fluorescence detection assembly, and the detection assembly reflector can reflect fluorescence excited by the first laser; simultaneously transmitting the side scattered light and fluorescence excited by the second laser; the fluorescence excited by the reflected first laser, the lateral scattered light excited by the second laser and the fluorescence are collimated into parallel light by the collimating lens, then the fluorescence of different wave bands is separated by the dichroic mirror, and the parallel light is detected by the detection modules of the fluorescence wavelength channels.
9. The optical detection device for a cell analyzer of claim 8, wherein the different fluorescence wavelength channels have different filter models.
10. A cell analyzer, comprising a reaction cell, a reagent supply device, a sampling unit, a sheath fluid supply device, a transport device, an analyzing unit, an output unit, and the optical detection device for a cell analyzer according to any one of claims 1 to 9.
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| CN202210316756.1A CN114577706A (en) | 2022-03-29 | 2022-03-29 | Optical detection device for cell analyzer and cell analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116908077A (en) * | 2023-09-08 | 2023-10-20 | 赛雷纳（中国）医疗科技有限公司 | Flow cytometer and control method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116908077A (en) * | 2023-09-08 | 2023-10-20 | 赛雷纳（中国）医疗科技有限公司 | Flow cytometer and control method thereof |
| CN116908077B (en) * | 2023-09-08 | 2023-11-24 | 赛雷纳（中国）医疗科技有限公司 | Flow cytometer and control method thereof |
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