CN206489074U - A kind of fluorescence detecting system for cytoanalyze - Google Patents
A kind of fluorescence detecting system for cytoanalyze Download PDFInfo
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- CN206489074U CN206489074U CN201720062734.1U CN201720062734U CN206489074U CN 206489074 U CN206489074 U CN 206489074U CN 201720062734 U CN201720062734 U CN 201720062734U CN 206489074 U CN206489074 U CN 206489074U
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Abstract
The utility model discloses a kind of fluorescence detecting system for cytoanalyze, the fluorescence detecting system includes object lens, optical fiber, concave surface microscope group, post lens group, light splitting microscope group, bandpass filter group, lens group and detector group etc..Multi-wavelength fluorescence signal is separated and imaged in 1 × magnifying power on detector by the utility model by wavelength in space, realizes the analysis detection of fluorescence；The utility model has a compact conformation, small volume, the advantages of debug simple；The utility model analysis detection wavelength of fluorescence number can also be actually needed according to client to be adjusted flexibly.
Description
Technical field
The utility model is related to medical Instrument field, more particularly to a kind of fluorescence detecting system for cytoanalyze.
Background technology
In biological and medical field, often using stream type cell analyzer come quantitative statisticses and analysis biological cell species with
Quantity.Stream type cell analyzer is using fluorometric reagent to sample dyeing, and the sample particles being colored pass through detection zone, utilizes simultaneously
Laser beam irradiating and detecting area, different types of sample particles being colored send the fluorescence signal of different wave length, and these are different
The fluorescence of wavelength is mixed collected by object lens.Fluorescence is divided into by different wave length using spectroscope and bandpass filter simultaneously
Fluorescence signal, the fluorescence signal of these different wave lengths is then analyzed one by one, is calculated by software, express statistic goes out grain in sample
The type and quantity of son.
United States Patent (USP) US 6683314 employs star light path system to collect detection fluorescence signal.In the utility model
In, fluorescence is collected using long-focal length lens, but aberration and angle of divergence problem of the fluorescence in transmitting procedure limit it and can detected
Wavelength of fluorescence number, therefore the utility model is only capable of fluorescence of the detection no more than 6 kinds of wavelength；Meanwhile, incide detector photosensitive
Fluorescence hot spot on face is more than 3mm.Therefore the technology that the utility model is proposed can only not only use the photomultiplier transit of high surface area
(PMT) is managed as detector, therefore whole system volume ratio is larger.
The A of CN 103091311 disclosed in Chinese patent literature devise a kind of phosphor collection detecting system, and the system is used
The fluorescence of wide spectral range is arranged in order out by the mode of biprism component light by wavelength in space.According to wavelength of fluorescence to be detected
Quantity uses the optical fiber group of identical quantity, to transmit fluorescence signal.Whole system will to the positional precision of biprism and optical fiber group
Ask higher, therefore cause the adjustment difficulty of whole system also further to increase.
Utility model content
The utility model is intended to that, there is provided a kind of fluorescence detecting system for cytoanalyze, the glimmering of detection can be realized
Optical wavelength number is not limited by its aberration and the angle of divergence in transmitting procedure, while whole system should have compact conformation, body
The advantages of product is small and debugs simple.
The purpose of this utility model is realized by following technology：
This fluorescence detecting system for cytoanalyze, it is characterised in that：Described fluorescence detecting system includes structure
Into the object lens of detection zone, optical fiber, concave surface microscope group, post lens group, light splitting microscope group, bandpass filter group, lens group and detector group,
The fluorescence detecting system being made up of above-mentioned component is to glimmering what is sent upon laser irradiation by the color card particle of detection zone
Light is collected detection；
Wherein：
The object lens are used to collect and collimate the fluorescence produced after sample particles Stimulated Light is excited；
The optical fiber includes incident end face and outgoing end face, and the optical fiber is used to transmit the fluorescence after collimating through object lens；
The concave surface microscope group include some concave mirrors be arranged in array, for reflected fluorescent light, the concave mirror it is recessed
Face is coated be all-trans film and its concave surface towards optical fiber；
The post lens group includes some plano-convex post lens be arranged in array, for correcting astigmatism, the post lens
Two faces are coated with anti-reflection film system；
The light splitting microscope group includes some spectroscopes being arranged in array, and each spectroscope can be according to wavelength of fluorescence to be measured
Difference plated film so that the fluorescence signal more than certain wavelength passes through, and reflected less than the fluorescence signal of the wavelength；
The bandpass filter group includes some bandpass filters being arranged in array, and each optical filter can be according to be measured
Wavelength of fluorescence distinguishes plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all by the anti-wavelength of fluorescence surveyed
Difference plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all reflected；
The lens group includes some non-spherical lenses being arranged in array, and two faces of each non-spherical lens are coated with
Anti-reflection film system, non-spherical lens images in the fluorescence hot spot on spectroscope on the photosurface of detector；
The detector group includes some detectors being arranged in array, and the detector correspondence band pass filter is marked off
Wavelength of fluorescence and set, the detector is used to receive fluorescence signal, and fluorescence signal is converted into electric signal.
Near the spectroscope is located at two times of focal positions of corresponding concave mirror.
Near the photosurface of the detector is located at two times of focal positions of corresponding non-spherical lens.
Near the spectroscope is located at two times of focal positions of corresponding non-spherical lens.
The incident end face of the optical fiber is located near the focal point of object lens；The outgoing end face of the optical fiber is located at concave mirror
Near at two times of focal positions.
The object lens, with higher numerical aperture (NA), NA is 0.5-1.4.
The optical fiber, with compared with low numerical aperture, NA is 0.05-0.15.
The non-spherical lens being arranged in array, with higher numerical aperture, NA is 0.5-0.9.
The convex curvature surface of post lens in the cylindrical lens array dorsad detector.
The photosurface of the detector array is towards lens；The detector is photomultiplier (PMT) or avalanche optoelectronic
Diode (APD).
The utility model proposed according to above technical scheme, the fluorescence detecting system uses Z-type light path, uses low numerical value
Aperture optical fiber output fluorescence signal, while using the angle of divergence and astigmatism of post lens correction fluorescence, non-spherical lens correction fluorescence
The aberrations such as the aberration and spherical aberration of signal, most fluorescence signal is arranged in order out in space by wavelength and is imaged with 1x magnifying powers at last
It is of substantially equal in the fluorescence spot size and optical fiber core diameter on the photosurface of detector, and on detector photosurface.Therefore originally
Utility model can be used small surface area detector (<1mm, such as avalanche diode APD) as detector, system is greatly reduced
Volume；The utility model can realize that the wavelength of fluorescence number of detection can need to increased or decrease according to client, can be achieved to one
Plant or the detection of multi-wavelength's fluorescence signal simultaneously, with more preferable client's adaptability；Meanwhile, optics pair of the present utility model
Adjustment precision is less demanding, with more preferable cost advantage, and the advantages of simple production process.
Brief description of the drawings
Fig. 1 is flow chamber, object lens and the optical fiber of a kind of fluorescence detecting system for cytoanalyze of the present utility model
Combination diagram；
Fig. 2 is a kind of fluorescence detecting system internal structure schematic diagram for cytoanalyze of the present utility model；
Fig. 3 is the light path schematic diagram of the transmission of fluorescence signal in systems in the utility model；
Wherein, 1A is flow chamber；1B is object lens；2 be optical fiber, and 21 be optical fiber incidence end face, and 22 be fiber exit end face；
3 be concave surface microscope group, including concave mirror 31,32,33,34 and 35；
4 be post lens group, including post lens 41,42,43,44 and 45；
5 be light splitting microscope group, including spectroscope 51,52,53,54 and 55；
6 be bandpass filter group, including bandpass filter 61,62,63,64 and 65；
7 be lens group, including non-spherical lens 71,72,73,74 and 75；
8 be detector group, including detector 81,82,83,84 and 85.
Embodiment
The utility model is described in further detail below by embodiment combination accompanying drawing.
This fluorescence detecting system for cytoanalyze, it is characterised in that：Described fluorescence detecting system includes structure
Into the object lens 1B of detection zone, optical fiber 2, concave surface microscope group 3, post lens group 4, light splitting microscope group 5, bandpass filter group 6, the and of lens group 7
Detector group 8, the fluorescence detecting system being made up of above-mentioned component by the color card particle of detection zone through laser to irradiating
The fluorescence sent afterwards is collected detection；
Wherein：
The object lens are used to collect and collimate the fluorescence produced after sample particles Stimulated Light is excited；
The optical fiber includes incident end face and outgoing end face, and the optical fiber is used to transmit the fluorescence after collimating through object lens；
The concave surface microscope group includes some concave mirrors for reflected fluorescence signal being arranged in array, the concave mirror
Concave surface is coated be all-trans film and its concave surface towards optical fiber；
The post lens group includes some plano-convex post lens be arranged in array, for correcting astigmatism, the post lens
Two faces are coated with anti-reflection film system；
The light splitting microscope group includes some spectroscopes being arranged in array, and each spectroscope can be according to wavelength of fluorescence to be measured
Difference plated film so that the fluorescence signal more than certain wavelength passes through, and reflected less than the fluorescence signal of the wavelength；
The bandpass filter group includes some bandpass filters being arranged in array, and each optical filter can be according to be measured glimmering
Optical wavelength distinguishes plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all reflected；
The lens group includes some non-spherical lenses being arranged in array, and two faces of each non-spherical lens are coated with
Anti-reflection film system, non-spherical lens will be imaged on the photosurface of detector by the fluorescence hot spot on spectroscope；
The detector includes some detectors being arranged in array, what the detector correspondence band pass filter was marked off
Wavelength of fluorescence and set, the detector is used to receive fluorescence signal, and fluorescence signal is converted into electric signal.
Such as Fig. 1 and a kind of as shown in Fig. 2 fluoroscopic examination system for cytoanalyze as described in the utility model embodiment
System, the system includes：
Flow chamber 1A, object lens 1B, optical fiber 2 (including incident end face 21 and outgoing end face 22), concave mirror are placed in light path
Group 3 (including 5 concave mirrors, are 31,32,33,34 and 35) respectively, post lens group 4 (including 5 post lens are 41 respectively, 42,
43rd, 44 and 45), light splitting microscope group 5 (including five spectroscopes, are 51,52,53,54 and 55) respectively, bandpass filter group 6 (including
5 band pass filters, are 61,62,63,64 and 65) respectively, lens group 7 (including 5 non-spherical lenses are 71 respectively, 72,
73rd, 74 and 75), and detector group 8 (including 5 detectors are 81 respectively, 82,83,84,85).
Wherein, the concave surface of concave mirror is coated with the membrane system that is all-trans, for reflected fluorescence signal；Post lens and non-spherical lens are equal
It is coated with anti-reflection film system；Spectroscope can distinguish plated film according to wavelength of fluorescence to be measured so that the fluorescence more than certain wavelength passes through, and is less than
The fluorescence signal of the wavelength can be reflected；Bandpass filter sector-meeting distinguishes plated film according to wavelength of fluorescence to be measured so that specific wavelength
Fluorescence passes through, and the fluorescence of other wavelength then all reflects；
Sample particles are dyed using fluorometric reagent, the sample particles after dyeing are by flow chamber 1A, while laser irradiates
Flow chamber, sample particles launch fluorescence signal after being stimulated, wherein fluorescence signal includes wideband multi-wavelength's fluorescence, wavelength point
Wei not λ1、λ2、λ3、λ4、λ5, and λ1>λ2>λ3>λ4>λ5；The glimmering of sample particles generation is collected by the object lens 1B with high-NA
Optical signal, and fluorescence signal is collimated；Fluorescence after collimation is by optical fiber incidence end face 21, into optical fiber 2, and from fiber exit
End face 22 is exported；Wherein, optical fiber incidence end face 21 is located near the focal position of object lens, and fiber exit end face 22 is located at concave mirror
Two times of focal positions near.
By fiber exit end face 22 export after fluorescence signal (include wavelength X1、λ2、λ3、λ4、λ5), transmit at a certain angle
To concave mirror 31 and reflected；Wherein incident angle is 10-35 °；Due to focal length of the concave mirror 31 on horizontal plane and vertical plane
Difference, causes fluorescence signal (to include wavelength X1、λ2、λ3、λ4、λ5) can both horizontally and vertically produced after the reflection of concave mirror 31
Astigmatism；
Fluorescence signal with astigmatism (includes wavelength X1、λ2、λ3、λ4、λ5), by post lens 41, then incident on light splitting
On mirror 51.Wherein, post lens 41 convex surface facing concave mirror, and convex curvature surface is that vertical direction is placed, and fluorescence signal passes through
During post lens, post lens are only focused to the fluorescence of vertical direction, without the fluorescent foci to horizontal direction, thus can be with
Correct fluorescence signal and (include wavelength X1、λ2、λ3、λ4、λ5) astigmatism；Meanwhile, spectroscope 51 is located at the two focus length of concave mirror 31
Near at position, the spot size both horizontally and vertically of the fluorescence signal so on spectroscope 51 substantially close to, and with
Optical fiber core diameter is of substantially equal.
Spectroscope 51 is coated with λ1It is high saturating, and less than λ1The high anti-membrane system of wavelength；Therefore wavelength X1Fluorescence pass through spectroscope
51, and wavelength is λ2、λ3、λ4、λ5It can then reflect.Wavelength X1Fluorescence pass sequentially through spectroscope 51, bandpass filter 61, then pass through
Non-spherical lens 71 is imaged on the photosurface of detector 81.The wherein photosurface of detector 81 and spectroscope 51 is respectively positioned on aspheric
Near at two times of focal positions of face lens 71, while the aberration such as spherical aberration and aberration of the recoverable fluorescence of non-spherical lens 71, because
This, the fluorescence hot spot on spectroscope 51 can be imaged on the photosurface of detector 81 with 1x magnifying powers.Detector 81 can be photoelectricity
Multiplier tube (PMT) or small surface area (<Avalanche photodide (APD) 1mm).
In brief, wavelength is λ1Fluorescence signal exported from optical fiber output end face 22, pass through concave mirror 31, post lens
41st, spectroscope 51, bandpass filter 61, eventually through non-spherical lens 71, the photosensitive of detector 81 is imaged in 1 × magnifying power
On face.And the fluorescence spot size and the core diameter of optical fiber on detector photosurface are of substantially equal.And wavelength is λ2, λ3, λ4, λ5
The fluorescence signal mirror 51 that is then split reflect.
Fluorescence signal after the mirror 51 that is split reflection (includes wavelength X2、λ3、λ4、λ5), transmitted with certain incidence angle to concave surface
Mirror 32 is simultaneously reflected；Wherein, its incidence angle is determined by angle of reflection of the fluorescence signal on spectroscope 51, and with the numerical value of optical fiber 2
Aperture NA is directly proportional；Wherein, near spectroscope 51 is located at two times of focal positions of concave mirror 32；Because concave mirror 32 is in level
Face is different with the focal length on vertical plane, causes fluorescence signal (to include wavelength X2、λ3、λ4、λ5) can be produced after the reflection of concave mirror 32
Astigmatism；
Fluorescence signal with astigmatism (includes wavelength X2、λ3、λ4、λ5), by post lens 42, then incident on spectroscope
On 52.Wherein, the convex surface of post lens 42 is placed towards concave mirror 32, and convex curvature surface for vertical direction, and fluorescence signal passes through
During post lens 42, post lens 42 are only focused to the fluorescence signal of vertical direction, without the fluorescent foci to horizontal direction, this
Sample can just correct fluorescence signal and (include wavelength X2、λ3、λ4、λ5) astigmatism；Meanwhile, spectroscope 52 is located at the two of concave mirror 32
Near at times focal position, the spot size both horizontally and vertically of the fluorescence so on spectroscope 52 substantially close to, with
Optical fiber core diameter is of substantially equal.
Spectroscope 52 is coated with wavelength X2It is high saturating, and less than λ2The high anti-membrane system of wavelength；Therefore wavelength X2Fluorescence can pass through
Spectroscope 52, and wavelength is λ3、λ4、λ5Fluorescence can then reflect.Wavelength X2Fluorescence pass sequentially through spectroscope 52, bandpass filter
On piece 62, the photosurface that detector 82 is imaged in eventually through non-spherical lens 72.The wherein photosurface of detector 82 and light splitting
Near mirror 52 is respectively positioned at two times of focal positions of non-spherical lens 72, at the same the spherical aberration of the recoverable fluorescence of non-spherical lens 72 and
The aberrations such as aberration, therefore, the fluorescence hot spot on spectroscope 52 can be imaged on the photosurface of detector 72 with 1x magnifying powers.Detection
Device 72 can for photomultiplier (PMT) or small surface area (<Avalanche photodide (APD) 1mm).
In brief, wavelength is λ2Fluorescence signal exported from optical fiber output end face 22, pass through concave mirror 31, post lens
41st, spectroscope 51, concave mirror 32, post lens 42, spectroscope 52, bandpass filter 62, eventually through non-spherical lens 72, with 1
× magnifying power is imaged on the photosurface of detector 82, and the fluorescence spot size on the photosurface of detector 82 and optical fiber core diameter
It is of substantially equal.And λ3, λ4, λ5The fluorescence signal mirror 52 that is then split reflect, continuation is transmitted in the optical path.
Fluorescence roundtrip transmission in the concave surface microscope group and light splitting microscope group for rise reflex, track is Z-type light path, in light
Lu Zhong, post lens correction astigmatism, the spherical aberration such as aspherical mirror spherical aberration corrector and aberration, in brief：
Wavelength is λ3Fluorescence signal exported from optical fiber output end face 22, first pass through concave mirror 31,32, post lens 41,42,
Spectroscope 51,52, then by concave mirror 33, post lens 43, spectroscope 53, by bandpass filter 63 and non-spherical lens 73,
Finally imaged in 1 × magnifying power on the photosurface of detector 83.And the fluorescence spot size on the photosurface of detector 83 with
Optical fiber core diameter is of substantially equal.Spectroscope 53 is coated with λ3Wavelength is high thoroughly, and less than λ3The high anti-membrane system of wavelength；Therefore λ4, λ5It is glimmering
The optical signal mirror 53 that is then split is reflected, and continuation is transmitted in the optical path.
Wavelength is λ4Fluorescence signal exported from optical fiber output end face 22, first pass through concave mirror 31,32,33, post lens 41,
42nd, 43, spectroscope 51,52,53, then by concave mirror 34, post lens 44, spectroscope 54, pass through bandpass filter 64 and aspheric
Face lens 74, are finally imaged on the photosurface of detector 84 with 1 × magnifying power.And the fluorescence hot spot on the photosurface of detector 84
Size and optical fiber core diameter are of substantially equal.Spectroscope 54 is coated with λ4Wavelength is high thoroughly, and less than λ4The high anti-membrane system of wavelength；Therefore λ5
The fluorescence signal mirror 54 that is then split reflect, continue to transmit in detecting system.
Wavelength is λ5Fluorescence signal exported from optical fiber output end face 22, first pass through concave mirror 31,32,33,34, post lens
41st, 42,43,44, spectroscope 51,52,53,54, then by concave mirror 35, post lens 45, spectroscope 55, pass through band pass filter
65 and non-spherical lens 75, finally imaged in 1 × magnifying power on the photosurface of detector 85 and on detector photosurface
Fluorescence spot size and optical fiber core diameter are of substantially equal.Spectroscope 55 is coated with λ5It is high saturating, and less than λ5The high anti-membrane system of wavelength.
, also can be according to client's actual demand and wavelength number to be measured, to realize fluorescence signal (bag in embodiment of above
Containing wavelength X1、λ2、λ3、λ4And λ5) in one or more wavelength fluorescence signal detection.
, also can be according to client's actual demand and wavelength number to be measured, by increasing concave mirror, post in embodiment of above
Lens, spectroscope, band pass filter, the quantity of non-spherical lens and detector, to realize the fluorescence signal more than 5 kinds of wavelength
Analysis detection, realizes that detection wavelength of fluorescence number is not limited by its angle of divergence and aberration in the transmission.
For in embodiments above, fluorescence signal is anti-back and forth in the concave surface microscope group and light splitting microscope group for rise reflex
The light path track for penetrating transmission is Z-type.Z-type track light path is now reduced to Fig. 3.Fig. 3 is concave surface microscope group of the present utility model and divided
Light microscopic group schematic diagram, wherein eliminating the post lens group in Fig. 2, bandpass filter group, lens group and detector group.Note, it is first
Imaged in after first being reflected first through concave mirror M (1) from the fluorescence signal of optical fiber output from spectroscope D (1), Fig. 1 concave mirrors M (1)
Do not draw in figure 3.Wherein：
A(0)：Fluorescence signal hot spot, can be fiber optic aperture；
A(n)：Spot diameter at n-th of spectroscope；
D(n)：N-th of spectroscope；
M(n)：N-th of concave mirror；
In this design, A (0) is fluorescence signal hot spot, and it is equal with optical fiber core diameter；The fluorescence signal hot spot of optical fiber output
After A (0) reflects through concave mirror M (1), at spectroscope D (1) place, imaging obtains hot spot A (1), and then hot spot A (1) is through concave mirror M
(2) at spectroscope D (2) place, imaging obtains hot spot A (2) after reflecting, the like, the hot spot A (n-1) at spectroscope D (n-1) place
Imaging obtains hot spot A (n) on spectroscope D (n) after being reflected through concave mirror M (n).In this design, A (1) is located at concave mirror M
(2) before, A (2) be located at concave mirror M (2) behind, the like A (n-1) be located at concave mirror M (n) before, A (n) be located at concave surface
Behind mirror M (n)；Meanwhile, dichronic mirror D (1) be located at concave mirror M (2) two focus length position near, the like, spectroscope D
(n-1) near at concave mirror M (n) two focus length position.
Fluorescence determines in concave mirror M (2) incidence angle by its angle of reflection at D (1) place, and with the numerical aperture of optical fiber into
Direct ratio, if the magnifying power of (1) is 1 from A (0) to A：M, then A (1) place incidence angle size equal to A (0) place incidence angle be multiplied by because
Sub- m.A (1) arrives A (2) in this design, and A (2) arrives A (3), the like, A (n) to A (n+1) magnifying power is equal to 1, i.e. m
Equal to 1.Therefore the fluorescence signal of optical fiber output can be arranged in order out by the utility model by wavelength in space, and with 1 × amplification
Rate is imaged on spectroscope.Meanwhile, spectroscope and detector are respectively positioned on two times of focal positions of corresponding non-spherical lens
Place, thus whole system the fluorescence signal of optical fiber output can be arranged in order open by wavelength in space and with 1X magnifying powers into
As on the photosurface of detector, and fluorescence spot size and optical fiber core diameter on detector photosurface can be made of substantially equal.
In summary, after the fluorescence signal comprising multiple wavelength incides concave mirror through optical fiber, by post lens group and point
Light microscopic group, each fluorescence is separated successively in space；In transmitting procedure, cylindrical mirror correction fluorescence signal astigmatism, aspherical mirror school
The aberration such as positive spherical aberration and aberration, fluorescence signal is arranged in order out by wavelength in space and incided accordingly with 1 × magnifying power
On the detector of wavelength；The wavelength of fluorescence number of network analysis detection can need to increased or decrease according to client, with more hospitable
Family adaptability；Meanwhile, optics of the present utility model is not high to adjustment required precision, with more preferable cost advantage, and life
Production. art.
Above content is to combine specific embodiment further detailed description of the utility model, it is impossible to assert
Specific implementation of the present utility model is confined to these explanations.For the utility model person of an ordinary skill in the technical field
For, without departing from the concept of the premise utility, some simple deduction or replace can also be made, should all be considered as category
In protection domain of the present utility model.
Claims (10)
1. a kind of fluorescence detecting system for cytoanalyze, it is characterised in that：Described fluorescence detecting system includes constituting
Object lens, optical fiber, concave surface microscope group, post lens group, light splitting microscope group, bandpass filter group and the detector group of detection zone, by above-mentioned structure
The fluorescence detecting system of part composition by the color card particle of detection zone in the fluorescence sent upon laser irradiation to receiving
Collection detection；
Wherein：
The object lens are used to collect and collimate the fluorescence produced after sample particles Stimulated Light is excited；
The optical fiber includes incident end face and outgoing end face, and the optical fiber is used to transmit the fluorescence after collimating through object lens；
The concave surface microscope group includes some concave mirrors be arranged in array, for reflected fluorescent light, and the concave surface of the concave mirror is plated
There are be all-trans film and its concave surface towards optical fiber；
The post lens group includes some plano-convex post lens compositions be arranged in array, for correcting astigmatism, for correcting picture
Scattered plano-convex post lens, described two faces of post lens are coated with anti-reflection film system；
The light splitting microscope group includes some spectroscopes being arranged in array, and each spectroscope can be distinguished according to wavelength of fluorescence to be measured
Plated film so that the fluorescence signal more than certain wavelength passes through, and reflected less than the fluorescence signal of the wavelength；
The bandpass filter group includes some bandpass filters being arranged in array, and each optical filter can be according to fluorescence to be measured
Wavelength distinguishes plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all reflected；
The lens group includes some non-spherical lenses being arranged in array, and two faces of each non-spherical lens are coated with anti-reflection
Membrane system, non-spherical lens images in the fluorescence hot spot on spectroscope on the photosurface of detector；
The detector group includes some detectors being arranged in array, and it is glimmering that the detector correspondence band pass filter is marked off
Optical wavelength and set, the detector is used to receive fluorescence signal, and fluorescence signal is converted into electric signal.
2. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The spectroscope
Near at two times of focal positions of corresponding concave mirror.
3. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The detector
Photosurface be located at corresponding non-spherical lens two times of focal positions near.
4. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The spectroscope
Near at two times of focal positions of corresponding non-spherical lens.
5. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, its feature exists：The optical fiber enters
End face is penetrated near the focal point of object lens；Near the outgoing end face of the optical fiber is located at two times of focal positions of concave mirror.
6. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The object lens,
With higher numerical aperture (NA), NA is 0.5-1.4.
7. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The optical fiber,
With compared with low numerical aperture, NA is 0.05-0.15.
8. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：Described is in array
The non-spherical lens of arrangement, with higher numerical aperture, NA is 0.5-0.9.
9. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：Described is in array
The convex curvature surface of the post lens of arrangement dorsad detector.
10. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：Described is in battle array
The photosurface of detector of arrangement is arranged towards lens；The detector is photomultiplier (PMT) or avalanche photodide
(APD)。
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| Application Number | Priority Date | Filing Date | Title |
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| CN201720062734.1U CN206489074U (en) | 2017-01-19 | 2017-01-19 | A kind of fluorescence detecting system for cytoanalyze |
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| CN201720062734.1U CN206489074U (en) | 2017-01-19 | 2017-01-19 | A kind of fluorescence detecting system for cytoanalyze |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706589A (en) * | 2017-01-19 | 2017-05-24 | 上海厦泰生物科技有限公司 | Fluorescence detection system used for cell analyzer |
| CN109444027A (en) * | 2018-12-26 | 2019-03-08 | 常州必达科生物科技有限公司 | A kind of particle analyzer and its optically detecting module |
-
2017
- 2017-01-19 CN CN201720062734.1U patent/CN206489074U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706589A (en) * | 2017-01-19 | 2017-05-24 | 上海厦泰生物科技有限公司 | Fluorescence detection system used for cell analyzer |
| CN106706589B (en) * | 2017-01-19 | 2023-08-25 | 上海厦泰生物科技有限公司 | Fluorescence detection system for cell analyzer |
| CN109444027A (en) * | 2018-12-26 | 2019-03-08 | 常州必达科生物科技有限公司 | A kind of particle analyzer and its optically detecting module |
| CN109444027B (en) * | 2018-12-26 | 2024-04-02 | 常州必达科生物科技有限公司 | Particle analyzer and optical acquisition module thereof |
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