CN109444027A - A kind of particle analyzer and its optically detecting module - Google Patents
A kind of particle analyzer and its optically detecting module Download PDFInfo
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- CN109444027A CN109444027A CN201811604107.1A CN201811604107A CN109444027A CN 109444027 A CN109444027 A CN 109444027A CN 201811604107 A CN201811604107 A CN 201811604107A CN 109444027 A CN109444027 A CN 109444027A
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- 239000002245 particle Substances 0.000 title claims abstract description 34
- 230000003287 optical effect Effects 0.000 claims abstract description 63
- 239000000523 sample Substances 0.000 claims description 49
- KPHWPUGNDIVLNH-UHFFFAOYSA-M diclofenac sodium Chemical compound [Na+].[O-]C(=O)CC1=CC=CC=C1NC1=C(Cl)C=CC=C1Cl KPHWPUGNDIVLNH-UHFFFAOYSA-M 0.000 claims description 35
- 238000001514 detection method Methods 0.000 claims description 22
- 230000000712 assembly Effects 0.000 claims description 8
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- 230000005284 excitation Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
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- 230000009977 dual effect Effects 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
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- 230000001413 cellular effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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Abstract
The invention discloses a kind of particle analyzer and its optically detecting modules, the particle analyzer includes flow chamber, the flow chamber has the first opposite side and second side, the optically detecting module includes the single element lens of the reflecting mirror positioned at the first side of flow chamber and second side positioned at flow chamber, the multiple light sources of the flow chamber are correspondingly formed multiple focal beam spots of non co axial after reflecting mirror reflects, and the multiple focal beam spot forms the light beam that multiple optical axises with single element lens are in different inclination angle after single element lens.The optically detecting module designed by using reflecting mirror plus single element lens, improve phosphor collection efficiency, so that instrument integrally has high sensitivity, improve signal-to-noise ratio, the crosstalk between different light paths and the collection of veiling glare signal are avoided the occurrence of, the complexity of whole optical texture is greatly reduced and system is assembled and debugging difficulty.
Description
Technical field
The present invention relates to optical instrument analysis technical field more particularly to a kind of particle analyzers and its optically detecting mould
Block, particle analyzer especially flow cytometer.
Background technique
Flow cytometry and flow cytometry are that one kind carries out quantitative analysis to unicellular or other biological particle and divides
The detection means of choosing, can be with the thousands of a cells of high speed analysis in the short time, and can measure from a cell simultaneously multiple
Parameter.Flow cytometer is referred to as the CT in laboratory, and cell sample liquid is focused to unicellular stream by the liquid fluid system of flow cytometer
Layer, cell queuing pass sequentially through laser irradiation area.Using laser irradiation sample, the scattering light of cell and the dye carried from cell
The fluorescence inspired on material is emitted centered on cell towards surrounding.Therefore, cell can be regarded as point light source, by fluorescence
Collection and analysis, to obtain the information of sample.Since fluorescence signal is very faint, and it is similar to point light source and is sent out towards surrounding
It penetrates.Therefore, reception fluorescence signal as much as possible is the key that improve flow cytometer detection performance.
Since many fluorescent dyes used in flow cytometer have unique spectral characteristic, and in order to biological thin
The specific phenotypic analysis of born of the same parents, it is often necessary to use more than one excitation or light source, can accurately just sort out different classes of
Cell.For Multi Colour Lasers flow cytometer, when flow cytometer configures multiple laser light sources, laser focus point edge
Flowing chamber axis genesis analysis, each laser focus point can inspire fluorescence and scattering light, be considered as flowing at this time
There are multiple point light sources on the axis of room.Every two luminous point is spaced between tens microns to 200 microns.Using multiple light sources
When, several fluorescence can be separated and detected by the fluorescent lifetime for each light source that is staggered.What kind of therefore made using optical system
The fluorescence signal that so compact light source issues, which spreads out, to be transferred into different optical filtering group modules, and between reduction
Crosstalk be technological difficulties on flow cytometer.
The fluorescence signal collected on the flow chamber of flow cytometer is generally coupled into multiple optical fiber ends by the prior art
It is interior, each fluorescence detection mould is then led out into using the fluorescence signal of different light sources as the light beam being spatially separating by optical fiber
In block.The optical fiber fluorescence collection system of existing flow cytometer has the problem that (1) optical system is excessively complicated, once by
To external action, such as temperature, vibration, liquid stream stability, it is easy to cause the fluorescence signal of cell losing on fiber end face
It loses.(2) since only fluorescence excitaton source is imaged on compact-sized fiber end face, the spacing of each point light source picture is separated not bery
Greatly, the fluorescence signal between different excitaton sources still remains crosstalk possibility, and simultaneously for optical fiber, the only angle of divergence is less than with a tight waist
The light beam of the core diameter of optical fiber can just be propagated in it, limit larger.Also have using non-fibre coupled in the prior art
Method collects the technology of fluorescence, but the telescope that is formed using multi-disc optical mirror slip of this method or microscope optical system come
Fluorescence is collected, entire optical texture is excessively complicated, brings certain difficulty to system assembly and debugging.
Summary of the invention
For overcome the deficiencies in the prior art, one of the objects of the present invention is to provide a kind of new structural particle analysis
The optically detecting module of instrument, particle analyzer especially flow cytometer, the existing multi-disc light of the optically detecting module reduction
The structure that fluorescence is collected in eyeglass space is learned, the complexity of whole optical texture is greatly reduced, can effectively overcome crosstalk phenomenon, is believed
Number collection efficiency height.
The second object of the present invention is to provide a kind of particle analyzer using above-mentioned optically detecting module, particle analysis
Instrument especially flow cytometer.
The purpose of the present invention is implemented with the following technical solutions:
A kind of optically detecting module of particle analyzer, the particle analyzer includes flow chamber, and the flow chamber has
The first opposite side and second side, the optically detecting module include positioned at the reflecting mirror of the first side of flow chamber and positioned at flowing
The single element lens of second side of room, the multiple light sources of the flow chamber are correspondingly formed the multiple poly- of non co axial after reflecting mirror reflects
Burnt hot spot, it is in different inclination angle that the multiple focal beam spot is correspondingly formed multiple optical axises with single element lens after single element lens
Light beam.
Preferably, the reflecting mirror has the rear surface backwards to the front surface of flow chamber and towards flow chamber, the preceding table
Face is the curvature spherical surface for being coated with reflectance coating, and the rear surface is plane.
Preferably, the single element lens are the plano-convex lens or concave-sphere coaxial with reflecting mirror.
A kind of particle analyzer, the particle analyzer include flow chamber and optical system, and the optical system includes light
Acquisition module is learned, the optically detecting module is above-mentioned optically detecting module.
Preferably, the optical system further includes light splitting detecting module, and the light splitting detecting module is used for saturating through monolithic
The light beam that mirror is formed after focusing is divided and is detected.
Preferably, the light splitting detecting module includes multiple light splitting probe assemblies along optical path direction setting, each light splitting
Probe assembly includes two to dichronic mirror and photodetector, and exposing to two should to the light beam transmission of the first wavelength range of dichronic mirror
Two detect to dichronic mirror and by corresponding photodetector, expose to two to dichronic mirror second wave length range light beam by two
It to next light splitting probe assembly and is split and detects again to dichroic mirror.
Preferably, the multiple light splitting probe assembly along optical path direction number consecutively and is divided into odd number of points optical detection component
It is divided probe assembly with even number, the odd number of points optical detection component is arranged side by side, and the even number light splitting probe assembly is arranged side by side,
Light beam roundtrip and by different odd number of points optical detections between odd number of points optical detection component and even number light splitting probe assembly
Component and different even numbers light splitting probe assembly are divided and are detected.
Preferably, the light splitting probe assembly further includes bandpass filter and condenser lens, the bandpass filter and poly-
Focus lens are sequentially arranged in two to dichronic mirror and light to the optical path direction of the light beam of the first wavelength range of dichronic mirror along transmission two
Between electric explorer.
Preferably, the light splitting detecting module further includes at least one total reflective mirror along optical path direction setting, described to be all-trans
Mirror is used to reflex to light beam the two of next light splitting probe assembly to dichronic mirror or next total reflective mirror.
Preferably, the light splitting detecting module further includes collimation lens, and the collimation lens is arranged in list along optical path direction
Between piece lens and multiple light splitting probe assemblies.
Compared with prior art, beneficial effects of the present invention include at least:
By using the optically detecting module that reflecting mirror plus single element lens design, phosphor collection efficiency is improved, so that instrument
Device integrally has high sensitivity, avoids the occurrence of the collection of the crosstalk phenomenon and veiling glare signal between different light paths, greatly reduces whole
The complexity and system of bulk optics structure are assembled and debugging difficulty.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the optically detecting module of the embodiment of the present invention.
Fig. 2 is the structural schematic diagram of the light splitting detecting module of one embodiment of the invention.
Fig. 3 is the structural schematic diagram of the light splitting detecting module of another embodiment of the present invention.
Fig. 4 is the structural schematic diagram of the light splitting detecting module of further embodiment of this invention.
Fig. 5 is the structural schematic diagram of the optical system of the particle analyzer of the embodiment of the present invention.
In figure: 10, flow chamber；20, optically detecting module；21, reflecting mirror；22, single element lens；30, it is divided detecting module；
31, it is divided probe assembly；311, two to dichronic mirror；312, bandpass filter；313, condenser lens；314, photodetector；
315, total reflective mirror；32, collimation lens；B, blue light exciting light source point；R, feux rouges exciting light source point.
Specific embodiment
Example embodiment is described more fully with reference to the drawings.However, example embodiment can be with a variety of shapes
Formula is implemented, and is not understood as limited to embodiment set forth herein；On the contrary, thesing embodiments are provided so that the present invention more
Fully and completely, and by the design of example embodiment comprehensively it is communicated to those skilled in the art.It is identical attached in figure
Icon note indicates same or similar structure, thus will omit repetition thereof.
The word of expression position and direction described in the present invention, is the explanation carried out by taking attached drawing as an example, but according to need
It can also make a change, done change is all contained in the scope of the present invention.
Referring to figs. 1 to Fig. 5, particle analyzer of the invention especially flow cytometer, particle analyzer includes flow chamber
10 and optical system.Flow chamber 10 has the first opposite side and second side, and cell to be analyzed or biological particle queuing pass through
Light source point is formed after flow chamber 10 and stimulated light excitation.Optical system includes optically detecting module 20, further comprises that light splitting is visited
Module 30 is surveyed, optically detecting module 20 is for being acquired the multiple light sources of flow chamber 10 and being formed with different inclination angle
Multiple light beams, light splitting detecting module 30 are used to carry out the light beam formed after the focusing of the single element lens 22 of optically detecting module 20
Light splitting and detection.
Referring to Fig.1, the optically detecting module 20 of particle analyzer includes reflecting mirror 21 and single element lens 22.Wherein, it reflects
Mirror 21 is located at the first side of flow chamber 10, and reflecting mirror 21 can be adhered to the first side of flow chamber 10, and reflecting mirror 21 has backwards
The front surface of flow chamber 10 and rear surface towards flow chamber 10, front surface is the curvature spherical surface for being coated with reflectance coating, and rear surface is
Plane.Single element lens 22 are located at second side of flow chamber 10, and in the present embodiment, single element lens 22 are coaxial with reflecting mirror 21 flat
Convex lens or concave-sphere, the multiple light sources of flow chamber 10 are correspondingly formed multiple focal beam spots of non co axial after the reflection of reflecting mirror 21,
Multiple focal beam spots are correspondingly formed the light beam that multiple optical axises with single element lens 22 are in different inclination angle after single element lens 22.Tool
For body, the focus of 22 the two of reflecting mirror 21 and single element lens is coaxial.Since multiple light sources are located at perpendicular to reflecting mirror 21
It in the vertical plane of optical axis, and is distributed at the multiple spot up and down of optical axis, the light that light source issues is focused on by reflecting mirror 21
The multiple picture points of separation in vertical optical axis plane at 22 front surface of single element lens, multiple picture point is due to being not in optical axis
On, the multiple picture points separated on far surface are imaged in through single element lens 22, that is, form the light beam of different inclination angle.The optics is adopted
Collection module 20 simplifies the structure that fluorescence is collected in existing multi-disc optical mirror slip space, greatly reduces answering for whole optical texture
Miscellaneous degree, avoids the occurrence of the collection of the crosstalk phenomenon and veiling glare signal between different light paths, and signal collection efficiency is high.
Specifically, by taking dual laser system as an example, blue laser and red laser irradiate flow chamber 10 respectively in Fig. 1
Cell stream, and be respectively formed by the blue light exciting light source point B of different wave length laser excitation and feux rouges exciting light source point R, two light
Source point differs close on the axis of flow chamber 10, and the fluorescence signal respectively issued focuses to single element lens 22 by reflecting mirror 21
Before mirror.Two light source points are imaged as two focal beam spots above and below 22 optical axis of single element lens, the two non co axials respectively
Focal beam spot two beams are formed after single element lens 22 focus again respectively with the optical axis of single element lens 22 in different inclination angle
Two beam focus on light beam.On the mirror surface of the collimation lens 32 of light splitting detecting module 30, apart tens millimeters of two beam fluorescent light beams,
And the distance can be gradually increased with the propagation distance of diagonal beam, and the final two light beams are visited by different light splitting respectively
Module 30 is surveyed to collect.
Above-mentioned optically detecting module 20 has at least the following advantages: 1, it is limited compared to existing fluorescence gathering system by fiber end face
The scheme of the propagation of fluorescence signal processed, optically detecting module 20 of the invention propagate fluorescence with can be realized the limitation of non-fiber end face
Signal, because the single element lens 22 are subjected to the numerical aperture of light beam and light beam end face is far longer than optical fiber, when entire light
When system is by extraneous interference, be not easy the phenomenon that cellular informatics loss occur so that instrument integrally have it is highly sensitive
Degree.2, it is designed by the optical texture of non co axial, the light beam that the light source activation at a distance of closer distance goes out is converted to larger
The diagonal beam at inclination angle increases signal-to-noise ratio so that it is entered in different collimation lenses 32, but also remaining spuious letter
Conversion number Jing Guo the single element lens 22, separates with fluorescent light beam, the light signal of each light source point is reliably visited
It measures, avoids the occurrence of the collection of the crosstalk phenomenon and veiling glare signal between different light paths.3, reflecting mirror 21 of the invention plus monolithic are saturating
The design scheme of mirror 22 enormously simplifies structure (telescope or the microscope light that fluorescence is collected in existing multi-disc optical mirror slip space
System), it greatly reduces the complexity of whole optical texture and system is assembled and debugging difficulty.
It should be noted that optically detecting module 20 of the invention is described by taking dual laser system as an example above,
But it is understood that the optically detecting module 20 can be also used for more laser irradiations such as three laser, four laser be
System equally can be realized the above technical effect with the separation of more light source points.
Fig. 2 is the structural schematic diagram of the light splitting detecting module 30 of one embodiment of the invention, and light splitting detecting module 30 includes
It further include collimation lens 32 along multiple light splitting probe assemblies 31 of optical path direction setting.Each light splitting probe assembly 31
It further comprise bandpass filter 312 and condenser lens 313 including two to dichronic mirror 311 and photodetector 314.
For being divided detecting module 30, the prior art is generally all-trans using each two to placement one before dichronic mirror 311
The mode of mirror 315 comes the signal distinguishing of the different wave length in fluorescence signal, therefore, passes through each bandpass filter 312
Light beam at least needs to undergo one time two reflection and the reflection of a total reflective mirror 315 to dichronic mirror 311, for example one has 8
The light splitting detecting module 30 of photodetector 314, fluorescent light beam need 8 times two reflections and 7 total reflective mirrors to dichronic mirror 311
315 reflection could make the fluorescence signal of different wave length distinguish, to have respectively entered corresponding photodetector
314, fluorescence signal loss is very big.
The present invention innovatively change light splitting detecting module 30 structure so that a branch of fluorescent light beam only need to by 7 times two to
The reflection of dichronic mirror 311 can separate the fluorescence signal of above-mentioned different wave length, enter respective photodetector 314,
Therefore, the structure of the light splitting detecting module 30 can be reduced to fluorescence signal order of reflection the half of the prior art, drop significantly
The low fluorescent signal decay as caused by multiple reflections, and entirely to be divided 30 more compact structure of detecting module, volume is smaller
Ingeniously, be conducive to assembly arrangement.
Specifically, expose to two to the light beam of the first wavelength range of dichronic mirror 311 transmit this two to dichronic mirror 311 simultaneously
Detected by corresponding photodetector 314, expose to two to dichronic mirror 311 second wave length range light beam by two to color separation
Mirror 311 reflexes to next light splitting probe assembly 31 and is split and detects again.Collimation lens 32 is arranged in along optical path direction
Between single element lens 22 and multiple light splitting probe assemblies 31, bandpass filter 312 and condenser lens 313 are along transmission two to dichronic mirror
The optical path direction of the light beam of 311 first wavelength range is sequentially arranged in two between dichronic mirror 311 and photodetector 314.
As shown in Fig. 2, multiple light splitting probe assemblies 31 are along optical path direction number consecutively 1 to 6, and it is divided into odd number light splitting and visits
It surveys component 31 and even number is divided probe assembly 31, odd number of points optical detection component 31 is arranged side by side, and even number is divided probe assembly 31 simultaneously
Row's setting, light beam roundtrip and by different between odd number of points optical detection component 31 and even number light splitting probe assembly 31
Odd number of points optical detection component 31 and different even numbers light splitting probe assembly 31 are divided and are detected.
More specifically, collimated 32 post-concentration of lens of fluorescent light beam of blue laser excitation is a branch of collimated light beam.
Wherein, the light beam less than certain numerical value wavelength (second wave length range) is numbered as the two of 1 light splitting probe assembly 31 to color separation
The two of the light splitting probe assembly 31 that it is 2 that mirror 311, which is reflexed to and numbered, are greater than numerical value wavelength (the first wave length model to dichronic mirror 311
Enclose) light beam be then transmitted through light splitting probe assembly 31 that number is 1 two to dichronic mirror 311, enter its corresponding band logical
On optical filter 312, bandpass filter 312 can exclude the signal of some spuious wavelength, and the light beam of the first wavelength range enters
In photodetector 314 with condenser lens 313, and then it is detected.For the two of the light splitting probe assembly 31 that number is 2
To dichronic mirror 311, the light beam for being less than certain numerical value wavelength (second wave length range) is reflected onto the light splitting probe assembly that number is 3
The two of 31 are to dichronic mirror 311, and the light beam for being greater than the numerical value wavelength (first wavelength range) is then transmitted into its corresponding band
In pass filter 312, the last focusing of line focus lens 313 is entered in photodetector 314.The light splitting detection that number is 4 to 6
Component 31 is using same light splitting detection process.
As needed, the number for being divided the light splitting probe assembly 31 of detecting module 30 can be further increased or be reduced, example
The setting of light splitting detecting module 30 as shown in Figure 3 extends to twenties kinds of fluorescence to realize there are three probe assembly 31 is divided
The detection of wavelength can also be reduced to the detection of two kinds of wavelength of fluorescence.It should be noted that respectively light splitting probe assembly 31 two to
For dichronic mirror 311 due to optical characteristics difference, first wavelength range and the second wave length range for respectively corresponding to light beam are generally different.
Light splitting detecting module 30 of the invention passes through special according to its corresponding optics to dichronic mirror 311 the two of different bandwidth
Property substantially symmetric placement, fluorescence signal, to 311 roundtrips of dichronic mirror, isolates the signal of different-waveband and enters each two
In self-corresponding detection channels.Compared with existing light splitting detecting module 30, which is detecting same fluorescence letter
When number type, fluorescence signal order of reflection can be reduced to the half of the prior art, greatly reduce and caused by multiple reflections
Fluorescent signal decay, be divided detecting module 30 structure it is also more compact.
As alternative embodiment, being divided detecting module 30 further includes at least one total reflective mirror 315 along optical path direction setting,
Total reflective mirror 315 is used to reflex to light beam the two of next light splitting probe assembly 31 to dichronic mirror 311 or next total reflective mirror
315.Specifically, referring to Fig. 4,
The difference of light splitting detecting module 30 shown in Fig. 4 and light splitting detecting module 30 shown in Fig. 2 is that number is 3
Light splitting probe assembly 31 has been substituted for total reflective mirror 315, and certainly, the part in the light splitting probe assembly 31 of other numbers can also replace
It is changed to total reflective mirror 315.By the total reflection of total reflective mirror 315, the two of the light splitting probe assembly 31 that light beam row to number is 4 are to color separation
When mirror 311, the light beam for being less than certain numerical value wavelength (second wave length range) is reflected onto the light splitting probe assembly 31 that number is 5
Two to dichronic mirror 311, and the light beam for being greater than the numerical value (first wavelength range) is then transmitted into point optical detection groups that number is 4
The two of part 31 finally go to its corresponding photodetector 314 in the bandpass filter 312 into dichronic mirror 311.Number is
The two of 5 light splitting probe assembly 31 to dichronic mirror 311 be less than certain numerical value wavelength light beam reflex to the last one two to point
Look mirror 311, and the light beam for being greater than certain numerical value wavelength then enters to its internal corresponding bandpass filter 312 and photodetector
In 314.
Fig. 5 is the structural schematic diagram of the optical system of the particle analyzer of the embodiment of the present invention, in Fig. 5, flow chamber 10
Two light source points are formed and single element lens 22 after the reflection of the reflecting mirror 21 of optically detecting module 20 and the focusing of single element lens 22
Optical axis be in different inclination angle two light beams, two light beams respectively after two collimation lenses 32 convergence be two collimated lights
Beam, finally, two collimated light beams are divided and are detected through two light splitting detecting modules 30 respectively.Particle analyzer can also be set
More light splitting detecting modules 30 are set, multiple light splitting detecting modules 30 are for more to being formed after the focusing of single element lens 22 respectively
A light beam is divided correspondingly.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case where, it can make changes, modifications, alterations, and variations to the above described embodiments in the range of invention, all these change
Change all should belong within the scope of protection of the claims of the present invention.
Claims (10)
1. a kind of optically detecting module of particle analyzer, the particle analyzer include flow chamber, the flow chamber has phase
Pair the first side and second side, which is characterized in that the optically detecting module include positioned at flow chamber the first side reflecting mirror
With the single element lens of the second side for being located at flow chamber, the multiple light sources of the flow chamber are correspondingly formed non-total after reflecting mirror reflects
Multiple focal beam spots of axis, it is in not that the multiple focal beam spot is correspondingly formed multiple optical axises with single element lens after single element lens
With the light beam at inclination angle.
2. the optically detecting module of particle analyzer according to claim 1, which is characterized in that the reflecting mirror has backwards
The front surface of flow chamber and rear surface towards flow chamber, the front surface is the curvature spherical surface for being coated with reflectance coating, it is described after table
Face is plane.
3. the optically detecting module of particle analyzer according to claim 1, which is characterized in that the single element lens be with it is anti-
Penetrate mirror coaxial plano-convex lens or concave-sphere.
4. a kind of particle analyzer, which is characterized in that the particle analyzer includes flow chamber and optical system, the optical system
System includes optically detecting module, and the optically detecting module is optically detecting module described in claims 1 to 3 any one.
5. particle analyzer according to claim 4, which is characterized in that the optical system further includes light splitting detection mould
Block, the light splitting detecting module is for being divided and being detected to the light beam formed after single element lens focus.
6. particle analyzer according to claim 5, which is characterized in that the light splitting detecting module includes along optical path direction
The multiple light splitting probe assemblies being arranged, each light splitting probe assembly include two to dichronic mirror and photodetector, expose to two to
The light beam of the first wavelength range of dichronic mirror transmits this and two detects to dichronic mirror and by corresponding photodetector, exposes to two
It is split to dichroic mirror to next light splitting probe assembly and again to the light beam of the second wave length range of dichronic mirror by two
And detection.
7. particle analyzer according to claim 6, which is characterized in that the multiple light splitting probe assembly is along optical path direction
Number consecutively is simultaneously divided into odd number of points optical detection component and even number light splitting probe assembly, and the odd number of points optical detection component is set side by side
It sets, the even number light splitting probe assembly is arranged side by side, and light beam is between odd number of points optical detection component and even number light splitting probe assembly
It roundtrip and is divided and is detected by different odd number of points optical detection component and different even number light splitting probe assemblies.
8. particle analyzer according to claim 6, which is characterized in that the light splitting probe assembly further includes bandpass filter
Piece and condenser lens, the bandpass filter and condenser lens along first wavelength range from transmission two to dichronic mirror light beam light
Road direction is sequentially arranged in two between dichronic mirror and photodetector.
9. particle analyzer according to claim 6, which is characterized in that the light splitting detecting module further includes along optical path side
To at least one total reflective mirror of setting, the total reflective mirror is used to reflex to light beam the two of next light splitting probe assembly to color separation
Mirror or next total reflective mirror.
10. particle analyzer according to claim 6, which is characterized in that the light splitting detecting module further includes that collimation is saturating
Mirror, the collimation lens are arranged between single element lens and multiple light splitting probe assemblies along optical path direction.
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| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
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