CN106706589A - Fluorescence detection system used for cell analyzer - Google Patents
Fluorescence detection system used for cell analyzer Download PDFInfo
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- CN106706589A CN106706589A CN201710038022.0A CN201710038022A CN106706589A CN 106706589 A CN106706589 A CN 106706589A CN 201710038022 A CN201710038022 A CN 201710038022A CN 106706589 A CN106706589 A CN 106706589A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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Abstract
The invention discloses a fluorescence detection system used for a cell analyzer. The fluorescence detection system comprises an objective lens, an optical fiber, a concave mirror assembly, a cylindrical lens assembly, a spectroscope assembly, a band-pass filter assembly, a lens assembly, a detector assembly and the like. According to the fluorescence detection system disclosed by the invention, a multi-wavelength fluorescent signal is separated in space according to wavelength and is imaged on a detector in 1*magnification, and fluorescence analysis and determination are realized; the fluorescence detection system has the advantages of compact structure, small size, simple debugging and the like; and the wavelength and number of fluorescence during analysis and detection also can be flexibly adjusted according to the actual needs of customers.
Description
Technical field
The present invention relates to medical Instrument field, more particularly to a kind of fluorescence detecting system for cytoanalyze.
Background technology
In biological and medical field, often using stream type cell analyzer come quantitative statisticses and analysis biological cell species and
Quantity., using fluorometric reagent to sample dyeing, the sample particles being colored are by detection zone for stream type cell analyzer, while utilizing
Laser beam irradiating and detecting area, different types of sample particles being colored send the fluorescence signal of different wave length, and these are different
The fluorescence of wavelength is mixed collected by object lens.Fluorescence is divided into by different wave length using spectroscope and bandpass filter simultaneously
Fluorescence signal, the fluorescence signal of these different wave lengths is then analyzed one by one, calculated by software, express statistic goes out grain in sample
The type and quantity of son.
United States Patent (USP) US 6683314 employs star light path system to collect detection fluorescence signal.In the invention, make
Fluorescence is collected with long-focal length lens, but aberration and angle of divergence problem of the fluorescence in transmitting procedure limit its fluorescence that can be detected
Wavelength number, therefore the invention is only capable of detection no more than 6 kinds of fluorescence of wavelength；Meanwhile, incide the fluorescence on detector photosurface
Hot spot is more than 3mm.Therefore the technology that the invention is proposed not only can only be using the photomultiplier (PMT) of high surface area as detection
Device, therefore whole system volume ratio is larger.
The A of CN 103091311 disclosed in Chinese patent literature devise a kind of phosphor collection detecting system, and the system is used
By wavelength be arranged in order out the fluorescence of wide spectral range in space by the mode of biprism component light.According to wavelength of fluorescence to be detected
Quantity transmits fluorescence signal using the optical fiber group of equal number.Whole system will to the positional precision of biprism and optical fiber group
Ask higher, therefore cause the adjustment difficulty of whole system also further to increase.
The content of the invention
It is contemplated that, there is provided a kind of fluorescence detecting system for cytoanalyze, the fluorescence ripple of detection can be realized
Number long is not limited by its aberration and the angle of divergence in transmitting procedure, while whole system should have compact conformation, small volume
The advantages of with debugging simple.
The purpose of the present invention is realized by following technology：
This fluorescence detecting system for cytoanalyze, it is characterised in that：Described fluorescence detecting system includes structure
Object lens, optical fiber, concave surface microscope group, post lens group, light splitting microscope group, bandpass filter group, lens group and detector group into detection zone,
The fluorescence detecting system being made up of above-mentioned component is glimmering what is sent upon laser irradiation to the color card particle by detection zone
Light is collected detection；
Wherein：
The object lens are used to collecting and collimating the fluorescence produced after sample particles Stimulated Light is excited；
The optical fiber includes incident end face and outgoing end face, and the optical fiber is used to transmit the fluorescence after being collimated through object lens；
The concave surface microscope group include some concave mirrors be arranged in array, for reflected fluorescent light, the concave mirror it is recessed
Face is coated be all-trans film and its concave surface towards optical fiber；
The post lens group includes some plano-convex post lens be arranged in array, for correcting astigmatism, the post lens
Two faces are coated with anti-reflection film system；
The light splitting microscope group includes some spectroscopes being arranged in array, and each spectroscope can be according to wavelength of fluorescence to be measured
Difference plated film so that the fluorescence signal more than certain wavelength passes through, and the fluorescence signal for being less than the wavelength is reflected；
The bandpass filter group includes some bandpass filters being arranged in array, and each optical filter can be according to be measured
Wavelength of fluorescence distinguishes plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all by the anti-wavelength of fluorescence surveyed
Difference plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all reflected；
The lens group includes some non-spherical lenses being arranged in array, and two faces of each non-spherical lens are coated with
Anti-reflection film system, non-spherical lens images on the photosurface of detector the fluorescence hot spot on spectroscope；
The detector group includes some detectors being arranged in array, and the detector correspondence band pass filter is marked off
Wavelength of fluorescence and set, the detector is used to receive fluorescence signal, and fluorescence signal is converted into electric signal.
Near the spectroscope is located at two times of focal positions of corresponding concave mirror.
Near the photosurface of the detector is located at two times of focal positions of corresponding non-spherical lens.
Near the spectroscope is located at two times of focal positions of corresponding non-spherical lens.
The incident end face of the optical fiber is located near the focal point of object lens；The outgoing end face of the optical fiber is located at concave mirror
Near at two times of focal positions.
The object lens, with numerical aperture (NA) higher, NA is 0.5-1.4.
The optical fiber, with compared with low numerical aperture, NA is 0.05-0.15.
The non-spherical lens being arranged in array, with numerical aperture higher, NA is 0.5-0.9.
The convex curvature surface of the post lens in the cylindrical lens array dorsad detector.
The photosurface of the detector array is towards lens；The detector is photomultiplier (PMT) or avalanche optoelectronic
Diode (APD).
According to the present invention that above technical scheme is proposed, the fluorescence detecting system uses Z-type light path, uses low numerical aperture
Optical fiber output fluorescence signal, while using the angle of divergence and astigmatism of post lens correction fluorescence, non-spherical lens correction fluorescence signal
Aberration and the aberration such as spherical aberration, most fluorescence signal is arranged in order out in space by wavelength and images in spy with 1x magnifying powers at last
Survey on the photosurface of device, and fluorescence spot size on detector photosurface is of substantially equal with optical fiber core diameter.Therefore it is of the invention
Can be used small surface area detector (<1mm, such as avalanche diode APD) system bulk is greatly reduced as detector；Should
Invention can realize that the wavelength of fluorescence number of detection can need to increased or decrease according to client, be capable of achieving to a kind of or simultaneously various
The detection of wavelength fluorescent signal, with more preferable client's adaptability；Meanwhile, optics of the invention is to adjusting required precision not
Height, with more preferable cost advantage, and the advantages of simple production process.
Brief description of the drawings
Fig. 1 is the group of the flow chamber, object lens and optical fiber of a kind of fluorescence detecting system for cytoanalyze of the invention
Close schematic diagram；
Fig. 2 is a kind of fluorescence detecting system internal structure schematic diagram for cytoanalyze of the invention；
Fig. 3 is the light path schematic diagram of fluorescence signal transmission in systems in the present invention；
Wherein, 1A is flow chamber；1B is object lens；2 is optical fiber, and 21 is optical fiber incidence end face, and 22 is fiber exit end face；
3 is concave surface microscope group, including concave mirror 31,32,33,34 and 35；
4 is post lens group, including post lens 41,42,43,44 and 45；
5 is light splitting microscope group, including spectroscope 51,52,53,54 and 55；
6 is bandpass filter group, including bandpass filter 61,62,63,64 and 65；
7 is lens group, including non-spherical lens 71,72,73,74 and 75；
8 is detector group, including detector 81,82,83,84 and 85.
Specific embodiment
The present invention is described in further detail below by specific embodiment combination accompanying drawing.
This fluorescence detecting system for cytoanalyze, it is characterised in that：Described fluorescence detecting system includes structure
Object lens 1B, optical fiber 2, concave surface microscope group 3 into detection zone, post lens group 4, light splitting microscope group 5, bandpass filter group 6, the and of lens group 7
Detector group 8, the fluorescence detecting system being made up of above-mentioned component irradiates to the color card particle by detection zone through laser
The fluorescence for sending afterwards is collected detection；
Wherein：
The object lens are used to collecting and collimating the fluorescence produced after sample particles Stimulated Light is excited；
The optical fiber includes incident end face and outgoing end face, and the optical fiber is used to transmit the fluorescence after being collimated through object lens；
The concave surface microscope group includes some concave mirrors for reflected fluorescence signal being arranged in array, the concave mirror
Concave surface is coated be all-trans film and its concave surface towards optical fiber；
The post lens group includes some plano-convex post lens be arranged in array, for correcting astigmatism, the post lens
Two faces are coated with anti-reflection film system；
The light splitting microscope group includes some spectroscopes being arranged in array, and each spectroscope can be according to wavelength of fluorescence to be measured
Difference plated film so that the fluorescence signal more than certain wavelength passes through, and it is less than the fluorescence signal reflection of the wavelength；
The bandpass filter group includes some bandpass filters being arranged in array, and each optical filter can be according to be measured glimmering
Optical wavelength distinguishes plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all reflected；
The lens group includes some non-spherical lenses being arranged in array, and two faces of each non-spherical lens are coated with
Anti-reflection film system, non-spherical lens will be imaged on the photosurface of detector by the fluorescence hot spot on spectroscope；
The detector includes some detectors being arranged in array, what the detector correspondence band pass filter was marked off
Wavelength of fluorescence and set, the detector is used to receive fluorescence signal, and fluorescence signal is converted into electric signal.
Such as Fig. 1 and as shown in Fig. 2 a kind of fluorescence detecting system for cytoanalyze as described in the embodiment of the present invention,
The system includes：
Flow chamber 1A, object lens 1B, optical fiber 2 (including incident end face 21 and outgoing end face 22), concave mirror are placed in light path
Group 3 (including 5 concave mirrors, are respectively 31,32,33,34 and 35), post lens group 4 (including 5 post lens are respectively 41,42,
43rd, 44 and 45), light splitting microscope group 5 (including five spectroscopes, are respectively 51,52,53,54 and 55), bandpass filter group 6 (including
5 band pass filters, are respectively 61,62,63,64 and 65), lens group 7 (including 5 non-spherical lenses are respectively 71,72,
73rd, 74 and 75), and detector group 8 (including 5 detectors are respectively 81,82,83,84,85).
Wherein, the concave surface of concave mirror is coated with the membrane system that is all-trans, for reflected fluorescence signal；Post lens and non-spherical lens are equal
It is coated with anti-reflection film system；Spectroscope can distinguish plated film according to wavelength of fluorescence to be measured so that the fluorescence more than certain wavelength passes through, and is less than
The fluorescence signal of the wavelength can be reflected；Bandpass filter sector-meeting distinguishes plated film according to wavelength of fluorescence to be measured so that specific wavelength
Fluorescence passes through, and the fluorescence of other wavelength then all reflects；
Sample particles are dyeed using fluorometric reagent, the sample particles after dyeing are by flow chamber 1A, while laser irradiates
Flow chamber, sample particles launch fluorescence signal after being stimulated, wherein fluorescence signal includes wideband multi-wavelength's fluorescence, wavelength point
Wei not λ1、λ2、λ3、λ4、λ5, and λ1>λ2>λ3>λ4>λ5；The glimmering of sample particles generation is collected by the object lens 1B with high-NA
Optical signal, and fluorescence signal is collimated；Fluorescence after collimation by optical fiber incidence end face 21, into optical fiber 2, and from fiber exit
End face 22 is exported；Wherein, optical fiber incidence end face 21 is located near the focal position of object lens, and fiber exit end face 22 is located at concave mirror
Two times of focal positions near.
Fluorescence signal after being exported by fiber exit end face 22 (includes wavelength X1、λ2、λ3、λ4、λ5), transmit at a certain angle
To concave mirror 31 and reflected；Wherein incident angle is 10-35 °；Due to focal length of the concave mirror 31 on horizontal plane and vertical plane
Difference, causes fluorescence signal (comprising wavelength X1、λ2、λ3、λ4、λ5) can both horizontally and vertically produced after the reflection of concave mirror 31
Astigmatism；
Fluorescence signal with astigmatism (includes wavelength X1、λ2、λ3、λ4、λ5), by post lens 41, then incident on light splitting
On mirror 51.Wherein, the curvature surface convex surface facing concave mirror and convex of post lens 41 is that vertical direction is placed, and fluorescence signal passes through
During post lens, post lens are only focused to the fluorescence of vertical direction, without the fluorescent foci to horizontal direction, thus can be with
Correction fluorescence signal (includes wavelength X1、λ2、λ3、λ4、λ5) astigmatism；Meanwhile, spectroscope 51 is located at the two focus length of concave mirror 31
Near at position, the spot size both horizontally and vertically of the fluorescence signal so on spectroscope 51 substantially close to, and with
Optical fiber core diameter is of substantially equal.
Spectroscope 51 is coated with λ1It is high saturating, and it is less than λ1Wavelength anti-membrane system high；Therefore wavelength X1Fluorescence pass through spectroscope
51, and wavelength is λ2、λ3、λ4、λ5Can then reflect.Wavelength X1Fluorescence pass sequentially through spectroscope 51, bandpass filter 61, then pass through
Non-spherical lens 71 is imaged on the photosurface of detector 81.The wherein photosurface of detector 81 and spectroscope 51 is respectively positioned on aspheric
Near at two times of focal positions of face lens 71, while the aberration such as spherical aberration and aberration of the recoverable fluorescence of non-spherical lens 71, because
This, the fluorescence hot spot on spectroscope 51 can be imaged on the photosurface of detector 81 with 1x magnifying powers.Detector 81 can be photoelectricity
Multiplier tube (PMT) or small surface area (<Avalanche photodide (APD) 1mm).
In brief, wavelength is λ1Fluorescence signal exported from optical fiber output end face 22, by concave mirror 31, post lens
41st, spectroscope 51, bandpass filter 61, eventually through non-spherical lens 71, the photosensitive of detector 81 are imaged in 1 × magnifying power
On face.And fluorescence spot size on detector photosurface is of substantially equal with the core diameter of optical fiber.And wavelength is λ2, λ3, λ4, λ5
The fluorescence signal mirror 51 that is then split reflect.
Fluorescence signal after the mirror 51 that is split reflection (includes wavelength X2、λ3、λ4、λ5), transmitted to concave surface with certain incidence angle
Mirror 32 is simultaneously reflected；Wherein, angle of reflection of its incidence angle by fluorescence signal on spectroscope 51 determine, and with the numerical value of optical fiber 2
Aperture NA is directly proportional；Wherein, near spectroscope 51 is located at two times of focal positions of concave mirror 32；Because concave mirror 32 is in level
Face is different with the focal length on vertical plane, causes fluorescence signal (comprising wavelength X2、λ3、λ4、λ5) can be produced after the reflection of concave mirror 32
Astigmatism；
Fluorescence signal with astigmatism (includes wavelength X2、λ3、λ4、λ5), by post lens 42, then incident on spectroscope
On 52.Wherein, the convex surface of post lens 42 is towards concave mirror 32, and convex curvature surface is vertical direction placement, and fluorescence signal passes through
During post lens 42, post lens 42 are only focused to the fluorescence signal of vertical direction, without the fluorescent foci to horizontal direction, this
Sample can just correct fluorescence signal (comprising wavelength X2、λ3、λ4、λ5) astigmatism；Meanwhile, spectroscope 52 is located at the two of concave mirror 32
Near at times focal position, the spot size both horizontally and vertically of the fluorescence so on spectroscope 52 substantially close to, with
Optical fiber core diameter is of substantially equal.
Spectroscope 52 is coated with wavelength X2It is high saturating, and it is less than λ2Wavelength anti-membrane system high；Therefore wavelength X2Fluorescence can pass through
Spectroscope 52, and wavelength is λ3、λ4、λ5Fluorescence can then reflect.Wavelength X2Fluorescence pass sequentially through spectroscope 52, bandpass filter
Piece 62, images on the photosurface of detector 82 eventually through non-spherical lens 72.The wherein photosurface of detector 82 and light splitting
Near mirror 52 is respectively positioned at two times of focal positions of non-spherical lens 72, at the same the spherical aberration of the recoverable fluorescence of non-spherical lens 72 and
The aberrations such as aberration, therefore, the fluorescence hot spot on spectroscope 52 can be imaged on the photosurface of detector 72 with 1x magnifying powers.Detection
Device 72 can for photomultiplier (PMT) or small surface area (<Avalanche photodide (APD) 1mm).
In brief, wavelength is λ2Fluorescence signal exported from optical fiber output end face 22, by concave mirror 31, post lens
41st, spectroscope 51, concave mirror 32, post lens 42, spectroscope 52, bandpass filter 62, eventually through non-spherical lens 72, with 1
× magnifying power is imaged on the photosurface of detector 82, and fluorescence spot size and optical fiber core diameter on the photosurface of detector 82
It is of substantially equal.And λ3, λ4, λ5The fluorescence signal mirror 52 that is then split reflect, continuation is transmitted in the optical path.
Fluorescence roundtrip transmission in the concave surface microscope group and light splitting microscope group for rise reflex, track is Z-type light path, in light
Lu Zhong, post lens correction astigmatism, the spherical aberration such as aspherical mirror spherical aberration corrector and aberration, in brief：
Wavelength is λ3Fluorescence signal exported from optical fiber output end face 22, first pass through concave mirror 31,32, post lens 41,42,
Spectroscope 51,52, then by concave mirror 33, post lens 43, spectroscope 53, by bandpass filter 63 and non-spherical lens 73,
Finally imaged on the photosurface of detector 83 with 1 × magnifying power.And the fluorescence spot size on the photosurface of detector 83 with
Optical fiber core diameter is of substantially equal.Spectroscope 53 is coated with λ3Wavelength is high, and is less than λ3Wavelength anti-membrane system high；Therefore λ4, λ5It is glimmering
The optical signal mirror 53 that is then split is reflected, and continuation is transmitted in the optical path.
Wavelength is λ4Fluorescence signal exported from optical fiber output end face 22, first pass through concave mirror 31,32,33, post lens 41,
42nd, 43, spectroscope 51,52,53, then by concave mirror 34, post lens 44, spectroscope 54, by bandpass filter 64 and aspheric
Face lens 74, are finally imaged on the photosurface of detector 84 with 1 × magnifying power.And the fluorescence hot spot on the photosurface of detector 84
Size is of substantially equal with optical fiber core diameter.Spectroscope 54 is coated with λ4Wavelength is high, and is less than λ4Wavelength anti-membrane system high；Therefore λ5
The fluorescence signal mirror 54 that is then split reflect, continue to be transmitted in detecting system.
Wavelength is λ5Fluorescence signal exported from optical fiber output end face 22, first pass through concave mirror 31,32,33,34, post lens
41st, 42,43,44, spectroscope 51,52,53,54, then by concave mirror 35, post lens 45, spectroscope 55, by band pass filter
65 and non-spherical lens 75, finally imaged in 1 × magnifying power on the photosurface of detector 85 and on detector photosurface
Fluorescence spot size is of substantially equal with optical fiber core diameter.Spectroscope 55 is coated with λ5It is high saturating, and it is less than λ5Wavelength anti-membrane system high.
In embodiment of above, fluorescence signal (bag can be also realized according to client's actual demand and wavelength number to be measured
Containing wavelength X1、λ2、λ3、λ4And λ5) in one or more wavelength fluorescence signal detection.
In embodiment of above, also can be according to client's actual demand and wavelength number to be measured, by increasing concave mirror, post
The quantity of lens, spectroscope, band pass filter, non-spherical lens and detector realizes the fluorescence signal more than 5 kinds of wavelength
Analysis detection, realizes that detection wavelength of fluorescence number is not limited by its angle of divergence and aberration in the transmission.
For in embodiments above, fluorescence signal is anti-back and forth in the concave surface microscope group and light splitting microscope group for rise reflex
The light path track for penetrating transmission is Z-type.Z-type track light path is now reduced to Fig. 3.Fig. 3 is concave surface microscope group of the invention and spectroscope
Group schematic diagram, wherein the post lens group in eliminating Fig. 2, bandpass filter group, lens group and detector group.Note, first from
The fluorescence signal of optical fiber output images in spectroscope D (1) place after being reflected through concave mirror M (1) first, and Fig. 1 concave mirrors M (1) do not exist
Drawn in Fig. 3.Wherein：
A(0)：Fluorescence signal hot spot, can be fiber optic aperture；
A(n)：Spot diameter at n-th spectroscope；
D(n)：N-th spectroscope；
M(n)：N-th concave mirror；
In this design, A (0) is fluorescence signal hot spot, and it is equal with optical fiber core diameter；The fluorescence signal hot spot of optical fiber output
After A (0) reflects through concave mirror M (1), at spectroscope D (1) place, imaging obtains hot spot A (1), and then hot spot A (1) is through concave mirror M
(2) at spectroscope D (2) place, imaging obtains hot spot A (2) after reflecting, the like, in the hot spot A (n-1) at spectroscope D (n-1) place
Imaging obtains hot spot A (n) on spectroscope D (n) after being reflected through concave mirror M (n).In this design, A (1) is positioned at concave mirror M
(2) before, A (2) behind concave mirror M (2), the like A (n-1) be located at concave mirror M (n) before, A (n) be located at concave surface
Behind mirror M (n)；Meanwhile, dichronic mirror D (1) at the two focus length position of concave mirror M (2) near, the like, spectroscope D
(n-1) near at the two focus length position of concave mirror M (n).
Fluorescence determines in the incidence angle of concave mirror M (2) by its angle of reflection at D (1) place, and with the numerical aperture of optical fiber into
Direct ratio, if the magnifying power of (1) is 1 from A (0) to A：M, then A (1) place incidence angle size equal to A (0) place incidence angle be multiplied by because
Sub- m.A (1) to A (2), A (2) to A (3) in this design, the like, the magnifying power of A (n) to A (n+1) is equal to 1, i.e. m
Equal to 1.Therefore by wavelength can be arranged in order out the fluorescence signal of optical fiber output in space by the present invention, and with 1 × magnifying power into
As on spectroscope.Meanwhile, spectroscope and detector are respectively positioned at two times of focal positions of corresponding non-spherical lens, because
By wavelength can be arranged in order open the fluorescence signal of optical fiber output in space and image in spy with 1X magnifying powers by this whole system
Survey on the photosurface of device, and the fluorescence spot size on detector photosurface can be made of substantially equal with optical fiber core diameter.
In sum, after the fluorescence signal comprising multiple wavelength incides concave mirror through optical fiber, by post lens group and point
Light microscope group, each fluorescence is separated successively in space；In transmitting procedure, cylindrical mirror correction fluorescence signal astigmatism, aspherical mirror school
The aberration such as positive spherical aberration and aberration, fluorescence signal is arranged in order out by wavelength in space and is incided accordingly with 1 × magnifying power
On the detector of wavelength；The wavelength of fluorescence number of network analysis detection can need to increased or decrease according to client, with more hospitable
Family adaptability；Meanwhile, optics of the invention is not high to adjustment required precision, with more preferable cost advantage, and production work
Skill.
Above content is to combine specific embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to protection of the invention
Scope.
Claims (10)
1. a kind of fluorescence detecting system for cytoanalyze, it is characterised in that：Described fluorescence detecting system includes constituting
The object lens of detection zone, optical fiber, concave surface microscope group, post lens group, light splitting microscope group, bandpass filter group and detector group, by above-mentioned structure
The fluorescence detecting system of part composition is received to the color card particle by detection zone in the fluorescence for sending upon laser irradiation
Collection detection；
Wherein：
The object lens are used to collecting and collimating the fluorescence produced after sample particles Stimulated Light is excited；
The optical fiber includes incident end face and outgoing end face, and the optical fiber is used to transmit the fluorescence after being collimated through object lens；
The concave surface microscope group includes some concave mirrors be arranged in array, for reflected fluorescent light, and the concave surface of the concave mirror is plated
There are be all-trans film and its concave surface towards optical fiber；
The post lens group includes some plano-convex post lens compositions be arranged in array, for correcting astigmatism, for correcting picture
Scattered plano-convex post lens, two faces of the post lens are coated with anti-reflection film system；
The light splitting microscope group includes some spectroscopes being arranged in array, and each spectroscope can be distinguished according to wavelength of fluorescence to be measured
Plated film so that the fluorescence signal more than certain wavelength passes through, and the fluorescence signal for being less than the wavelength is reflected；
The bandpass filter group includes some bandpass filters being arranged in array, and each optical filter can be according to fluorescence to be measured
Wavelength distinguishes plated film so that the fluorescence of specific wavelength passes through, and the fluorescence of other wavelength is all reflected；
The lens group includes some non-spherical lenses being arranged in array, and two faces of each non-spherical lens are coated with anti-reflection
Membrane system, non-spherical lens images on the photosurface of detector the fluorescence hot spot on spectroscope；
The detector group includes some detectors being arranged in array, and it is glimmering that the detector correspondence band pass filter is marked off
Optical wavelength and set, the detector is used to receive fluorescence signal, and fluorescence signal is converted into electric signal.
2. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The spectroscope
Near at two times of focal positions of corresponding concave mirror.
3. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The detector
Photosurface be located at corresponding non-spherical lens two times of focal positions near.
4. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The spectroscope
Near at two times of focal positions of corresponding non-spherical lens.
5. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, its feature exists：The optical fiber enters
End face is penetrated to be located near the focal point of object lens；Near the outgoing end face of the optical fiber is located at two times of focal positions of concave mirror.
6. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The object lens,
With numerical aperture (NA) higher, NA is 0.5-1.4.
7. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：The optical fiber,
With compared with low numerical aperture, NA is 0.05-0.15.
8. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：It is described in array
The non-spherical lens of arrangement, with numerical aperture higher, NA is 0.5-0.9.
9. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：It is described in array
The convex curvature surface of the post lens of arrangement dorsad detector.
10. a kind of fluorescence detecting system for cytoanalyze as claimed in claim 1, it is characterised in that：It is described in battle array
The photosurface of detector of arrangement is arranged towards lens；The detector is photomultiplier
Or avalanche photodide (APD) (PMT).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710038022.0A CN106706589B (en) | 2017-01-19 | 2017-01-19 | Fluorescence detection system for cell analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710038022.0A CN106706589B (en) | 2017-01-19 | 2017-01-19 | Fluorescence detection system for cell analyzer |
Publications (2)
| Publication Number | Publication Date |
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| CN106706589A true CN106706589A (en) | 2017-05-24 |
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| CN110361074A (en) * | 2018-03-26 | 2019-10-22 | 华中科技大学 | A kind of photoelectric liquid-levle detection device |
| CN111208108A (en) * | 2020-02-27 | 2020-05-29 | 光越科技(深圳)有限公司 | Fluorescence detection method, system and device |
| CN112782141A (en) * | 2020-12-29 | 2021-05-11 | 中国科学院合肥物质科学研究院 | Plastic rapid classification equipment based on fluorescence method |
| CN112805543A (en) * | 2018-10-12 | 2021-05-14 | 株式会社日立高新技术 | Dichroic mirror array and photodetection device |
| CN113899677A (en) * | 2021-09-17 | 2022-01-07 | 桂林优利特医疗电子有限公司 | Reflective light splitting module and light splitting method for flow cytometer detection |
| CN114136867A (en) * | 2021-11-23 | 2022-03-04 | 清华大学 | Optical path design method and device for fluorescence dispersion of flow cytometer |
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| CN113899677A (en) * | 2021-09-17 | 2022-01-07 | 桂林优利特医疗电子有限公司 | Reflective light splitting module and light splitting method for flow cytometer detection |
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| CN114136867B (en) * | 2021-11-23 | 2023-10-03 | 清华大学 | Optical path design method and device for fluorescence dispersion of flow cytometer |
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