CN104458540B - Blood analyser flow chamber - Google Patents
Blood analyser flow chamber Download PDFInfo
- Publication number
- CN104458540B CN104458540B CN201410727173.3A CN201410727173A CN104458540B CN 104458540 B CN104458540 B CN 104458540B CN 201410727173 A CN201410727173 A CN 201410727173A CN 104458540 B CN104458540 B CN 104458540B
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- Prior art keywords
- micropore
- gem
- flow chamber
- installing hole
- hgb
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Abstract
The present invention discloses a kind of blood analyser flow chamber, the blood analyser flow chamber upper right is provided with a HGB transmitting tube installing hole, left side is provided with a HGB reception pipe installing hole, one electrode slice installing hole is arranged at adjacent HGB reception pipes installing hole bottom, there is a sample port in electrode slice installing hole lower left, there is a filter washing water inlet on right side, respectively there is a quartz window piece before and after below electrode slice installing hole, in sample port side and with the coaxial line of quartz window piece center on Kurt gem micropore is installed, Kurt gem micropore is processed by gem piece.It is an advantage of the current invention that：Flow chamber process structure is relatively easy, without sheath flow tube, without blast tube and low cost.Diluted cup is used in combination with by the present invention with flow chamber so that electrical impedance is detected and optical signal detecting is carried out in a part simultaneously, while saving material, also saves sample and reagent, and the working time of instrument greatly shortens.
Description
Technical field
The present invention relates to medical apparatus and instruments, especially a kind of blood analyser flow chamber.
Background technology
Flow chamber (Flow Chamber) is the core component of differential hematology analyzer.Traditional flow chamber is by sample
Transparent, the stable materials such as the composition such as pipe, sheath liquid pipe and nozzle, conventional optical glass, quartz are made, and design and make very smart
Carefully, be liquid fluid system heart.Sample cell stores sample, and individual cells suspension is projected from sample cell under flow stream pressure effect；
Sheath fluid flows to spray orifice from surrounding by sheath liquid pipe, projects from nozzle after being enclosed in sample periphery.In order to ensure that liquid stream is steady liquid, typically
Limit flow stream velocity and be less than 10m/s.Due to the effect of sheath fluid, detected cell is limited on the axis of liquid stream.On flow chamber
Equipped with piezoquartz, vibration can be occurred by oscillator signal.
Hole of the single cell suspension in cell flow chamber by sheath flow liquid wrapping by the indoor certain pore size that flows, detection zone
At the center in the hole, cell here is intersected with laser vertical, and under sheath flow liquid constraint, cell is arranged in single file and passes sequentially through laser
Detection zone.Sheath fluid stream wraps sample stream and makes sample stream be maintained at the axis direction of liquid stream, ensure that each cell by swashing
The time in light irradiation area is equal.The single center for flowing through the hole of cell, flow velocity are slower, thus cell can be collected by long according to the time
Cell signal luminous flux it is big, mix Radix Rumiciss collecting lens, very high detection sensitivity and certainty of measurement can be obtained.Therefore will be many
The different optical signal of angle is integrated and leukocyte can be divided into five classes.Flow chamber aperture have 60 μm, 100 μm, 150 μm, 250
μm etc. it is various.The miniature instrument general fixing device flow chamber of certain pore size.
Due to test cell volume in micron order, and flow chamber receiving light according to when need special shape and printing opacity
Degree, therefore small, high-precision flow chamber determines its difficult processing, complex structure and high cost.At present, it is high in technical requirements
In the case of it is domestic be difficult to find the company that can meet processing request, foreign technology is ripe, but its processing cost is very high.
The content of the invention
It is an object of the invention in order to overcome the deficiencies in the prior art, for the flowing of existing differential hematology analyzer
The problem of room difficult processing and high cost, there is provided a kind of structure is relatively easy and the flowing of the differential hematology analyzer of low cost
Room.
The technical scheme is that：A kind of blood analyser flow chamber, the blood analyser flow chamber upper right set
There is a HGB transmitting tube installing hole, left side is provided with a HGB reception pipe installing hole, and adjacent HGB reception pipes installing hole bottom has one
Individual electrode slice installing hole, has a sample port in electrode slice installing hole lower left, and there are a filter washing water inlet, electrode slice in right side
Below installing hole before and after respectively have a quartz window piece, in sample port side and with the coaxial line of quartz window piece center on
Kurt gem micropore is installed, Kurt gem micropore is processed by gem piece, and diameter is 50 μm -250 μm.In Ku Er
One hemolysin entrance is arranged at special gem micropore bottom, has a removal waste fluid mouth below hemolysin entrance.
The application of the flow chamber is sample blood and diluent, and to enter flowing from sample and diluent entrance indoor, and from molten
Sanguinin entrance injects hemolysin, and mixes.Then certain wavelength is launched by the HGB transmitting tubes in HGB transmitting tube installing holes
Light, light pass through mixed sample liquid, by the HGB reception pipes collection optical signal in HGB reception pipe installing holes, draw sample
HGB parameters.Then the indoor sample liquid that flows enters sample port by Kurt gem micropore, passes through micropore in sample liquid
When, the impedance in micropore can be changed, the change of impedance is collected by the electrode slice in electrode slice installing hole, so as to record by micro-
The hemocyte number in hole.While sample liquid passes through Kurt gem micropore, another road laser is injected from quartz window, is passed through
Kurt gem micropore is irradiated on the hemocyte by micropore, then by quartz window, is injected into optical signal collection system,
Form the optical signal of cell.After sampling end-of-job, remaining mixed liquor is discharged by after removal waste fluid, while being entered by cleanout fluid
Mouth suction cleanout fluid is used to clean inner chamber.
Differential hematology analyzer flow chamber is characterized in that its structure is by original three grouping blood analysers diluted cup
Structure adds 2 quartz window pieces for being coated with anti-reflection film, this stone before and after the center of Kurt gem micropore after improvement
English diaphragm one side plating plating anti-reflection film, the effect of anti-reflection film is the intensity for reducing reflected light, so as to increase the intensity of transmitted light, is made
Optical system imaging is apparent.The direction of coated surface dorsad Kurt gem micropore orienting reflex mirror during installation.It is close with resin glue
Seal the marginal portion of its surrounding so as to be capable of being fixed on five classification flow chambers of sealing, accomplish the effect of water-proof light permeable.
Kurt gem micro-pore diameter can be 70 μm, 80 μm, 100 μm.
It is an advantage of the current invention that：Flow chamber process structure is relatively easy, without sheath flow tube, without blast tube and low cost.This
Invention is not used separately using two parts of traditional flow chamber and diluted cup, but diluted cup is combined with flow chamber
Use so that electrical impedance is detected and optical signal detecting is carried out in a part simultaneously, while saving material, also saves sample
This and reagent, and the working time of instrument greatly shorten.
Description of the drawings
Fig. 1 is a structural representation of blood analyser flow chamber of the present invention；
Fig. 2 is the side structure schematic diagram of blood analyser flow chamber of the present invention.
In figure:100 represent sample and diluent entrance, 101 expression HGB reception pipe installing holes, 102 expression HGB transmitting tubes
Installing hole, 103 represent that electrode slice installing hole, 104 represent that sample port, 105 represent that filter washing water inlet, 106 represent Kurt gem
Micropore, 107 represent that hemolysin entrance, 108 represent that removal waste fluid mouth, 109 represent that the first quartz window piece, 110 represent that second is quartzy
In diaphragm, wherein Fig. 2, arrow represents laser light incident direction.
Specific embodiment
First pass through sample and diluent entrance 100 and sample (blood sample) is added to flow chamber, add diluent to mix afterwards, lead to
After crossing the addition hemolysin of hemolysin entrance 107, a negative pressure is had at sample port 104 by sample and the mixed liquor of other solution
Extract out from flow chamber, according to Coulter principle, can now produce leukogenic DC signals；In combination with the optics designed for which
System, when laser after the light path for designing by the first quartz window piece 109 can that focus is converged in Kurt gem be micro-
The center in hole, the hot spot of convergence are emitted onto whole Kurt gem micropore.When cell passes through from Kurt gem micropore
When, a light scattering signal will be produced, this light scattering signal will be kept by being coated with the second quartz window piece 110 of anti-reflection film
Light signal strength is unattenuated, then the light scattering signal of cell is converged to the machine of a diameter of 0.5mm through well-designed light path
On tool aperture, light scattering signal that other are not tested cells in micropore is so allowed for by mechanical aperture to sheltering from, also
So that spurious signal does not affect follow-up light path, the light scattering signal of final tested cell changes into parallel optical signal output
To on photodetector, photodetector is by the change for having an electric current so as to transmitting follow-up amplifying circuit.Cell passes through
During micropore, a light scattering signal can be produced in forward and backward, therefore be dissipated with reference to DC signals, forward light scattering and backward light
Penetrate signal and leukocyte can be divided into lymphocyte, acidophil, basophilic leukocyte, mononuclear cell and neutrophilic granulocyte.
The flow chamber process structure of the present invention is relatively easy, without the need for sheath flow liquid and low cost.
The preferred embodiments of the present invention are the foregoing is only, the patent of the present invention is not thereby limited, it is every using this
Equivalent structure or equivalent flow conversion that bright description and accompanying drawing content are made, or directly or indirectly it is used in other correlation necks
Domain, is included within the scope of the present invention.
Claims (4)
1. a kind of blood analyser flow chamber, it is characterised in that：The blood analyser flow chamber upper right is provided with a HGB and sends out
Pipe installing hole is penetrated, left side is provided with a HGB reception pipe installing hole, and an electrode slice peace is arranged at adjacent HGB reception pipes installing hole bottom
, there is a sample port in dress hole in electrode slice installing hole lower left, and there is a filter washing water inlet on right side, below electrode slice installing hole
Respectively have a quartz window piece in front and back, in sample port side and with the coaxial line of quartz window piece center on Ku Er is installed
Special gem micropore, has a hemolysin entrance in micropore bottom, has a removal waste fluid mouth below hemolysin entrance.
2. blood analyser flow chamber according to claim 1, it is characterised in that：Kurt gem micropore is added by gem piece
Work is formed, and diameter is 50 μm -250 μm.
3. blood analyser flow chamber according to claim 1, it is characterised in that：Kurt gem micro-pore diameter is 100 μm.
4. blood analyser flow chamber according to claim 1, it is characterised in that：The application of the flow chamber be sample blood and
Diluent enters flowing interior from sample and diluent entrance (100), and injects hemolysin from hemolysin entrance (107), and mixes
It is even；Then launch the light of certain wavelength by the HGB transmitting tubes in HGB transmitting tube installing holes (102), light is through after mixing
Sample liquid, by HGB reception pipe installing holes (101) HGB reception pipes collection optical signal, draw the HGB parameters of sample；Then
The indoor sample liquid of flowing passes through Kurt gem in sample liquid by Kurt gem micropore (106) into sample port (104)
During micropore (106), the impedance in micropore can be changed, the change of impedance is collected by the electrode slice in electrode slice installing hole (103),
So as to record the hemocyte number by micropore；While sample liquid passes through Kurt gem micropore (106), another road laser
Inject from the first quartz window piece (109), be irradiated on the hemocyte by micropore by Kurt gem micropore (106), so
Afterwards by the second quartz window piece (110), optical signal collection system is injected into, forms the optical signal of cell；When sampling work knot
Remaining mixed liquor is discharged by Shu Hou by removal waste fluid mouth (108), while being used for by filter washing water inlet (105) suction cleanout fluid clear
Wash inner chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410727173.3A CN104458540B (en) | 2014-12-04 | 2014-12-04 | Blood analyser flow chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410727173.3A CN104458540B (en) | 2014-12-04 | 2014-12-04 | Blood analyser flow chamber |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104458540A CN104458540A (en) | 2015-03-25 |
| CN104458540B true CN104458540B (en) | 2017-03-29 |
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ID=52904951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410727173.3A Expired - Fee Related CN104458540B (en) | 2014-12-04 | 2014-12-04 | Blood analyser flow chamber |
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| Country | Link |
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| CN (1) | CN104458540B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106769805B (en) * | 2017-01-22 | 2019-06-18 | 江西特康科技有限公司 | Cell count sorter |
| CN107449716A (en) * | 2017-07-25 | 2017-12-08 | 深圳市孔雀生物科技有限公司 | A kind of sorting unit of flow cytometer |
| CN107843542B (en) * | 2017-11-27 | 2023-05-30 | 桂林优利特医疗电子有限公司 | Sample cup for blood cell analyzer |
| CN111656161B (en) | 2018-04-28 | 2024-02-20 | 深圳迈瑞生物医疗电子股份有限公司 | Sample analyzer abnormality alarming method, system and storage medium |
| CN108663306B (en) * | 2018-07-27 | 2024-02-09 | 北京指真生物科技有限公司 | Flow cytometry analysis device and compensation method |
| CN108982338A (en) * | 2018-09-08 | 2018-12-11 | 长春迈克赛德医疗科技有限公司 | A kind of production method of particle imaging chamber and particle imaging chamber |
| CN109490175A (en) * | 2018-10-17 | 2019-03-19 | 南宁优康电子科技有限公司 | Cell count sorter |
| CN113376080A (en) * | 2021-06-11 | 2021-09-10 | 宋卓 | Liquid flow device and analytical sorting equipment comprising same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5730951A (en) * | 1980-08-04 | 1982-02-19 | Toa Medical Electronics Co Ltd | Blood analyzer |
| CN203572827U (en) * | 2013-06-26 | 2014-04-30 | 无锡和瑞盛光电科技有限公司 | Blood cell analysis device |
| CN204255817U (en) * | 2014-12-04 | 2015-04-08 | 南昌百特生物高新技术股份有限公司 | Blood analyser flow chamber |
-
2014
- 2014-12-04 CN CN201410727173.3A patent/CN104458540B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5730951A (en) * | 1980-08-04 | 1982-02-19 | Toa Medical Electronics Co Ltd | Blood analyzer |
| CN203572827U (en) * | 2013-06-26 | 2014-04-30 | 无锡和瑞盛光电科技有限公司 | Blood cell analysis device |
| CN204255817U (en) * | 2014-12-04 | 2015-04-08 | 南昌百特生物高新技术股份有限公司 | Blood analyser flow chamber |
Non-Patent Citations (3)
| Title |
|---|
| "BC-5500五分类血细胞分析仪性能评价";李炜煊 等;《现代医学仪器与应用》;20081031;第20卷(第3期);第26-30页 * |
| "MONITORING OF RED BLOOD CELL AGGREGABILITY IN A FLOW-CHAMBER BY COMPUTERIZED IMAGE ANALYSIS";Shuqi Chen et al.;《Clinical Hemorheology》;19941231;第14卷(第4期);第497-508页 * |
| "当代五分类血细胞分析仪技术原理分析";伍柏青 等;《实验与检验医学》;20110831;第29卷(第4期);第391-394页 * |
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| CN104458540A (en) | 2015-03-25 |
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Granted publication date: 20170329 Termination date: 20211204 |