CN113376080A - Liquid flow device and analytical sorting equipment comprising same - Google Patents
Liquid flow device and analytical sorting equipment comprising same Download PDFInfo
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- CN113376080A CN113376080A CN202110652066.9A CN202110652066A CN113376080A CN 113376080 A CN113376080 A CN 113376080A CN 202110652066 A CN202110652066 A CN 202110652066A CN 113376080 A CN113376080 A CN 113376080A
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- 238000001514 detection method Methods 0.000 claims abstract description 9
- 238000004458 analytical method Methods 0.000 claims abstract description 3
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- 239000012530 fluid Substances 0.000 claims description 18
- 239000002245 particle Substances 0.000 claims description 9
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects thereof, e.g. conductivity or capacity
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- G01N15/01—
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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- G01N2015/1028—
Abstract
The invention relates to a liquid flow device, which comprises a liquid drop generating assembly, a flow chamber assembly, a sample feeding device and a nozzle assembly, wherein the liquid drop generating assembly is connected with the sample feeding device, the flow chamber assembly is provided with a first passage, the far end of the sample feeding device extends into the first passage, and the nozzle assembly is arranged at the far end of the flow chamber assembly. The analysis and sorting equipment comprises the liquid flow device and a sample loading device connected with the liquid flow device, and further comprises a detection module, a control module and a sorting module. The invention can realize the breaking of the jet flow formed by the nozzle component into the liquid drops by arranging the liquid drop generating component, and the liquid drop generating component has small volume, small required space of the layout, simple structure and convenient installation. The arrangement of the flow chamber component enables the sample liquid flow to realize focusing during flowing, bubble discharging is convenient and fast, and stability is high.
Description
Technical Field
The invention relates to the field of analytical sorting equipment, in particular to a liquid flow device and analytical sorting equipment comprising the same.
Background
At present, the cells or particles are detected and analyzed by a dynamic method, that is, the cells or other particles in the sample to be detected flow through a specific detection area "one by one" at a constant speed, then a detection device is used to observe various performance characterization parameters of each cell or other particle flowing through the detection area, and a computer is used to perform large-scale data processing and classification on the observed results. In order to realize the constant speed of the cells or particles flowing through a specific detection area one by one, the laminar flow "focusing" force of the external sheath fluid is usually adopted to realize the flow, and the core flow device for realizing the "focusing" function in the traditional analytical sorting equipment has complex structure, low stability and large layout space requirement, so that the flow device capable of solving the problems needs to be designed.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, it is an object of the present invention to provide a fluidic device and an analytical sorting apparatus incorporating the same that address one or more of the problems of the prior art.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the liquid flow device comprises a liquid drop generation assembly, a flow chamber assembly, a sample feeding device and a nozzle assembly, wherein the liquid drop generation assembly is connected with the sample feeding device, the flow chamber assembly is provided with a first passage through which liquid flows, the far end of the sample feeding device extends into the first passage, the nozzle assembly is arranged at the far end of the flow chamber assembly, the flow chamber assembly is configured to gather the liquid flow flowing out of the sample feeding device, the nozzle assembly is provided with a second passage and is configured to form the liquid flow into a jet flow, and the liquid drop generation assembly is configured to break the jet flow into a liquid drop flow.
Furthermore, the liquid drop generating assembly comprises at least one piezoelectric ceramic arranged outside the sample feeding device and at least one insulating ceramic connected with the outside of the sample feeding device.
Further, the flow chamber assembly comprises a first body and a second body connected with the first body, wherein the first body is detachably or integrally connected with the second body; the first flow through hole comprises a first flow through hole formed in the first body and a third flow through hole formed in the second body, the first flow through hole is communicated with the third flow through hole, at least one liquid inlet is formed in the first body, and the liquid inlet is communicated with the first flow through hole.
Further, the inner diameter of the third through-flow hole is smaller than the inner diameter of the first through-flow hole.
Further, the nozzle assembly comprises a nozzle body, the second passage comprises an overflowing hole and a jet hole which are communicated with each other, and the hole diameter of the overflowing hole is gradually reduced from the near end to the far end.
Further, the nozzle assembly is also provided with a sealing groove, and the sealing groove is formed at the near end of the overflowing hole and used for assembling a sealing ring.
Further, the nozzle assembly is removably attached to a mounting assembly that includes a mounting body having at least one support member configured to apply pressure to and lift the nozzle assembly when in place, such that the nozzle assembly abuts the distal end of the flow chamber assembly when lifted.
Further, the liquid flow device further comprises a liquid drop charging device, the liquid drop charging device comprises a power-on end and a grounding end connected with one part of the power-on end, and the other part of the power-on end is connected with a sample feeding device.
And the near end of the flow chamber assembly is also connected with a mounting seat, and a joint assembly is rotatably arranged on the mounting seat and is connected with the near end of the sample feeding device so as to rotate the joint assembly to enable the sample feeding device to be fixed by the joint assembly.
The analyzing and sorting equipment comprises the liquid flow device and a sample loading device connected with the liquid flow device, and further comprises a detection module, a control module and a sorting module, wherein sample liquid is transferred to the liquid flow device from the sample loading device, the detection module is used for detecting at least one characteristic of biological particles in the sample liquid, the liquid flow device is used for focusing the sample liquid, generating a liquid droplet flow and charging liquid droplets, the control module sorts and sorts the liquid droplets according to the at least one characteristic of the biological particles in the liquid droplets, and the sorting module places the liquid droplets into a specified position by utilizing the sorting and sorting of the liquid droplets.
Compared with the prior art, the invention has the following beneficial technical effects:
the arrangement of the liquid drop generating assembly can realize that jet flow formed by the nozzle assembly is broken into liquid drops, and the liquid drop generating assembly is small in size, small in required space of layout, simple in structure and convenient to install.
And (II) the sample liquid flow is focused when flowing due to the arrangement of the flow chamber component, the air bubbles are conveniently discharged, and the stability is high.
And the nozzle assembly is detachably arranged in the fixing assembly, and the positioning precision of the nozzle assembly during installation can be improved by adding the supporting piece in the fixing assembly, so that the nozzle assembly is more stable to be disassembled and installed.
(IV) through setting up liquid drop charging device, further reduced the volume of whole liquid stream device, the installation of the liquid stream device of being convenient for, the sampling device contacts with sheath liquid (electrically conductive) to form the positive pole or the negative pole of induction electric field, make the liquid drop realize being charged, thereby be convenient for categorised the sorting.
Drawings
FIG. 1 is a schematic diagram showing the structure of a liquid flow device and an analytical sorting apparatus including the same according to an embodiment of the present invention.
FIG. 2 is a schematic cross-sectional view of a fluidic device and an analytical sorting apparatus including the same according to an embodiment of the present invention.
FIG. 3 is a schematic view showing a partial structure of a liquid flow device and an analytical sorting apparatus including the same according to an embodiment of the present invention.
FIG. 4 shows a schematic view of the connection of a nozzle assembly to a stationary assembly in a fluidic device and an analytical sorting apparatus incorporating the device according to an embodiment of the present invention.
FIG. 5 is a schematic diagram showing the structure of a stationary assembly in a fluidic device and an analytical sorting apparatus incorporating the same according to an embodiment of the present invention.
FIG. 6 is a schematic diagram showing the construction of a nozzle assembly in a fluidic device and an analytical sorting apparatus incorporating the device according to an embodiment of the present invention.
FIG. 7 shows a schematic view of a nozzle assembly in a fluidic device and an analytical sorting apparatus incorporating the same, in partial cross-section, according to an embodiment of the present invention.
FIG. 8 is a schematic view showing a partial structure of a fixing member body in a liquid flow device and an analyzing and sorting apparatus including the same according to an embodiment of the present invention.
FIG. 9 shows a schematic view of a support in another embodiment of a fluidic device and analytical sorting apparatus incorporating the device according to embodiments of the present invention.
In the drawings, the reference numbers: 100. an external connector; 101. a mounting seat; 102. an inner connecting piece; 2. a droplet generation assembly; 200. a first insulating ceramic; 201. piezoelectric ceramics; 202. a second insulating ceramic; 203. a seal member; 3. a flow chamber assembly; 300. a first body; 301. a first flow through hole; 302. a second flow through hole; 303. a third flow-through hole; 304. a second body; 3041. an extension portion; 305. a liquid inlet; 4. a sample introduction device; 5. a nozzle assembly; 501. a nozzle body; 502. assembling a groove; 503. a sealing groove; 504. an overflowing hole; 505. a jet hole; 506. opening a hole; 6. a fixing assembly; 601. a fixing member body; 602. an installation port; 603. a support member; 604. a limiting block; 6041. a protrusion; 605. a base; 606. a cylindrical member; 6061. a first part; 6062. a second section; 607. a spring; 7. a base; 801. a power-on end; 802. and a ground terminal.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the liquid flow device and the analytical sorting device including the same according to the present invention are further described in detail with reference to the accompanying drawings and the detailed description. The advantages and features of the present invention will become more apparent from the following description. It is to be noted that the drawings are in a very simplified form and are all used in a non-precise scale for the purpose of facilitating and distinctly aiding in the description of the embodiments of the present invention. To make the objects, features and advantages of the present invention comprehensible, reference is made to the accompanying drawings. It should be understood that the structures, ratios, sizes, and the like shown in the drawings and described in the specification are only used for matching with the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the implementation conditions of the present invention, so that the present invention has no technical significance, and any structural modification, ratio relationship change or size adjustment should still fall within the scope of the present invention without affecting the efficacy and the achievable purpose of the present invention.
As used in this specification and the appended claims, the singular forms "a", "an", and "the" include plural referents unless the content clearly dictates otherwise. As used in this specification and the appended claims, the term "or" is generally employed in its sense including "and/or" unless the content clearly dictates otherwise, the term "proximal" generally being the end proximate to the loading direction and the term "distal" generally being the end proximate to the loading direction. Taking fig. 1 as an example, the upper side of fig. 1 is the proximal end, and the lower side of fig. 1 is the distal end.
The first embodiment is as follows:
referring to fig. 1 and 2, the entire fluid flow device of the present invention is disposed on a base 7, the fluid flow device includes a droplet generation assembly 2, a flow chamber assembly 3, a sample injection device 4, and a nozzle assembly 5, the droplet generation assembly 2 is connected to the sample injection device 4, the sample injection device 4 is preferably a sample injection needle for communicating with the sample injection device and implementing sample injection of a sample fluid into an internal passage of the sample injection needle, the flow chamber assembly 3 has a first passage through which the fluid can flow, a distal end of the sample injection device 4 extends into the first passage, the nozzle assembly 5 is disposed at a distal end of the flow chamber assembly 3, the flow chamber assembly 3 is configured to collect the fluid flowing out from the sample injection device 4 in a straight line, the nozzle assembly 5 has a second passage and is configured to form the fluid flow into a jet, and the droplet generation assembly 2 is configured to break the jet into a droplet flow.
The specific structure of the droplet generation module 2 is described below as follows:
referring to fig. 2, the droplet generation assembly 2 includes at least one piezoelectric ceramic 201 disposed outside the sample injection device 4 and at least one insulating ceramic connected to the outside of the sample injection device 4.
Specifically, with reference to fig. 2, in the liquid flow device according to the first embodiment of the present invention, the insulating ceramics are a first insulating ceramic 200 and a second insulating ceramic 202, the piezoelectric ceramic 201 is disposed between the first insulating ceramic 200 and the second insulating ceramic 202, and the piezoelectric ceramic 201 is used for generating high frequency vibration (0 to 100kHz) to generate a stable axial disturbance to the jet flow generated by the nozzle assembly 5, so that the continuous jet flow is broken into a stable liquid flow.
The specific structure of the flow cell assembly 3 is described below as follows:
with continued reference to fig. 1 and fig. 2, the flow chamber assembly 3 includes a first body 300 and a second body 304 connected to the first body 300, and the first body 300 and the second body 304 are integrally connected by means of optical cement, so that the stability of the collected liquid flow is improved.
Referring to fig. 2 and fig. 3, the first passage includes a first through hole 301 formed in the first body 300 and a third through hole 303 formed in the second body 304, wherein the first through hole 301 is a tapered hole, the distal end of the sample injection device 4 extends into the first through hole 301, the first through hole 301 is communicated with the second through hole 302, and the inner diameter of the third through hole 303 is smaller than the inner diameter of the first through hole 301. Liquid inlets 305 are respectively formed in the left side and the right side of the first body 300, and two adjacent liquid inlets 305 are oppositely arranged and are respectively communicated with the first flow through hole 301. The liquid inlet 305 may further remove bubbles from the liquid flow to improve the stability of the liquid flow, and the removal of bubbles may be performed simultaneously with the sheath liquid, so as to have high efficiency and rapidity.
Further, a second through hole 302 is formed at the proximal end of the third through hole 303, the second through hole 302 is used for further gathering the sample liquid flow and converging the liquid flow to the third through hole 303, and the second through hole 302 is also used for smooth transition between the first through hole 301 and the third through hole 303, so that focusing is more stable.
Further, an extension 3041 is formed at the distal end of the second body 304 and extends in the direction of the second flow through hole 302, the extension 3041 is used for limiting the nozzle body 501 after the nozzle body 501 is inserted into the fixing member body 601, and after the end of the nozzle body 501 having the flow through hole 504 abuts against the inner side of the extension 3041, the sealing groove 503, the flow through hole 504, the jet hole 505, the first flow through hole 301, and the second flow through hole 302 of the nozzle body 501 are all located on the same axis.
Further, referring to fig. 2, the droplet generation assembly 2 further includes a sealing member 203, in the liquid flow device according to the first embodiment of the present invention, the sealing member 203 is preferably an O-ring, the distal end of the sample injection device 4 is matched with a hole in the center of the O-ring, and the outer side of the O-ring is in interference fit with the inner diameter of the hole of the first through hole 301, so as to fix the O-ring and achieve the sealing connection with the first through hole 301.
The specific structure of the nozzle assembly 5 is described below:
referring to fig. 4, 6 and 7, the nozzle assembly 5 includes a nozzle body 501, and the nozzle body 501 is integrally formed by ceramic die-casting. The nozzle body 501 is provided with an assembly groove 502, and the assembly groove 502 is formed along the width direction of the nozzle body 501. The second passage comprises an overflowing hole 504 and a jet hole 505 which are communicated with each other, the aperture of the overflowing hole 504 is gradually reduced from the near end to the far end, the jet hole 505 is used for forming the liquid flow flowing through the nozzle assembly 5 into jet flow, and the aperture of the jet hole 505 can be 50um-200 um.
Further, with continued reference to fig. 4, 6 and 7, the nozzle assembly 5 further includes a seal groove 503, the seal groove 503 is formed at the proximal end of the overflow hole 504, and the seal groove 503 is coaxially disposed with the overflow hole 504 for assembling a seal ring.
Further, with continuing reference to fig. 4, fig. 6 and fig. 7, the distal end of the jet hole 505 is further provided with an opening 506 communicating with the jet hole 505, and the aperture of the opening 506 is larger than that of the jet hole 505, so that the jet does not contact the hole wall of the opening 506 when being ejected.
The nozzle assembly 5 is detachably connected to the fixing assembly 6, and the specific structure of the fixing assembly 6 is described as follows:
further, with continuing reference to fig. 4 and 5, the fixing assembly 6 includes a fixing member body 601, the fixing member body 601 has an installation opening 602 into which the front end of the nozzle body 501 can be inserted, two opposite sides of the installation opening 602 respectively have a limiting block 604, the limiting blocks 604 and the fixing member body 601 are integrally or detachably disposed, a protrusion 6041 is formed by extending opposite sides of the limiting blocks 604, and the protrusion 6041 is used for matching with the assembling groove 502 formed on the nozzle body 501.
Further, with continuing reference to fig. 4 and fig. 5, the fixing body 601 has four supporting members 603, in the fluid flow device according to the embodiment of the present invention, the supporting members 603 are spring-loaded plungers, mounting holes (not shown) for mounting the supporting members 603 are formed on the fixing body 601, the supporting members 603 protrude from the surface of the fixing body 601 by a certain height in an initial state, and can be compressed downward in the height direction when being compressed, and reset to bounce again when not being compressed. The support 603 is configured to press the nozzle assembly 5 when the nozzle assembly 5 is in place, bringing the nozzle assembly 5 into abutment with the distal end of the flow chamber assembly 3.
Of course, in other embodiments of the present invention, the mounting position of the support 603 is not limited to the end near the nozzle body 501 having the overflowing hole 504, but may be arranged in a plurality of ways at the end far from the nozzle body 501 having the overflowing hole 504, so that the nozzle body 501 is lifted by the support 603 to avoid the tilting problem, and the nozzle body 501 is always in contact with the distal end of the second body 304 in the flow chamber assembly 3.
Further, with continued reference to fig. 4 and fig. 5, the liquid flow device further includes a liquid droplet charging device, the liquid droplet charging device includes a power-on terminal 801 and a ground terminal 802 connected to the power-on terminal 801, wherein the power-on terminal 801 can be connected to a charging voltage to charge the liquid droplets in the sample feeding device 4 with positive charges or negative charges, so that the liquid droplet flow near the electric field is charged. Specifically, the sample injection device 4 is made of stainless steel, and has a conductive function, and is in contact with the sheath fluid (conductive) to form a positive electrode or a negative electrode of the induced electric field.
Further, a mounting seat 101 is further connected to the proximal end of the flow chamber assembly 3, and the joint assembly is rotatably disposed on the mounting seat 101 and connected to the proximal end of the sample introduction device 4, so that the joint assembly is rotated to fix the sample introduction device 4 by the joint assembly.
Specifically, referring to fig. 1, the connector assembly includes an external connector 100 and an internal connector 102 engaged with the external connector 100, the internal connector 102 is a supporting member such as a rubber member, a hole for a sample injection device 4 to pass through is formed in the center of the internal connector 102, the external connector 100 is in threaded connection with the mounting seat 101, and when the external connector 100 is rotated, the external connector 100 applies radial pressure to the internal connector 102 while rotating, so that the internal connector 102 is radially pressed and the aperture of the hole is reduced, thereby holding the sample injection device 4 tightly.
The specific working process of the liquid flow device in the first embodiment is as follows:
the internal passage of the sample introduction device 4 is introduced with a sample liquid flow, meanwhile, the sheath liquid is introduced into the first through hole 301 of the flow chamber component 3 through the liquid inlet 305, the sample liquid is focused on the second through hole 302 and then enters the third through hole 303, and then enters the jet hole 505 of the nozzle component 5 to form a jet flow, and the jet flow is broken into a stable liquid drop flow by causing stable axial disturbance to the jet flow through the high-frequency vibration of the piezoelectric ceramic 201.
The liquid drop flows down and then is conducted with the liquid drop charging device, the grounding plate is placed at the position of the liquid drop breaking point, when positive pressure or negative pressure pulse is applied to the charging electrode, electrostatic induction is formed between the liquid flow and the grounding plate, positive charge or negative charge accumulated by the electrostatic induction can be taken away when the liquid drop breaks, and the liquid drop is charged at the moment. The charged liquid drops pass through a high-voltage electrostatic field formed by the high-voltage deflection plate and are under the action of an electric field force, the liquid drops with negative charges deflect towards the positive pole direction of the deflection plate, and the liquid drops with negative charges deflect towards the negative pole direction of the deflection plate.
Referring to fig. 4 and 5, when the nozzle assembly 5 is installed, one end of the nozzle body 501 having the overflowing hole 504 is inserted into the installation opening 602 of the fixing member body 601, the nozzle body 501 gradually contacts the support 603 during the insertion movement, and presses the support 603 to descend until the end of the nozzle body 501 having the overflowing hole 504 contacts the extension 3041, and the assembly groove 502 on the nozzle body 501 is also located right below the protrusion 6041 of the fixing member body 601, so that the support 603 is not limited by pressure and lifts the nozzle body 501, the assembly groove 502 of the nozzle body 501 is matched with the protrusion 6041 to abut, and the nozzle body 501 cannot be pulled out. When the nozzle body 501 is matched with the nozzle body, an O-shaped sealing ring arranged in the sealing groove 503 of the nozzle body 501 is attached to the inner side of the sealing groove 503, so that the sealing performance of one end of the nozzle body 501, which forms jet flow, is ensured.
With continued reference to fig. 4 and 5, when the nozzle body 501 needs to be replaced, downward pressure is applied to the nozzle body 501 to disengage the assembling groove 502 of the nozzle body 501 from the protrusion 6041, at this time, the nozzle body 501 can be pulled out, and then the nozzle assembly 5 can be replaced by inserting the nozzle body 501 again according to the above-mentioned installation process.
Accordingly, referring to fig. 8 and 9, in order to avoid the height of the supporting member 603 protruding from the surface of the fixing member body 601 from being inconsistent, it is impossible to provide a consistent supporting force for the nozzle assembly 5. Therefore, in other embodiments of the present invention, the supporting member 603 may be replaced by a machined cylindrical member 606, and the cylindrical member 606 is integrally connected by a first part 6061 and a second part 6062 which are coaxially arranged. Wherein the first portion 6061 has an outer diameter that is less than the outer diameter of the second portion 6062. The lower surface of the second part 6062 abuts one end of the spring 607, and the other end of the spring 607 abuts the base 605.
Similarly, in order to accommodate the column 606, the spring 607 and the base 605, an accommodating cavity (not shown) is formed in the fixing member body 601, and the accommodating cavity accommodates the spring 607 and the column 606 and is closed by the base 605.
The uniform height of the surface of the first part 6061 of the four posts 606 extending out of the fixture body 601 provides uniform support for the nozzle assembly 5.
Correspondingly, the invention also provides an analysis and sorting device, which comprises the liquid flow device and a sample loading device connected with the liquid flow device, and further comprises a detection module, a control module and a sorting module, wherein the sample liquid is transferred from the sample loading device to the liquid flow device, the detection module is used for detecting at least one characteristic of biological particles in the sample liquid, the liquid flow device is used for focusing the sample liquid, generating a liquid drop flow and charging the liquid drop, the control module sorts and sorts the liquid drop according to the at least one characteristic of the liquid drop, and the sorting module places the liquid drop into a specified position by utilizing the sorting and sorting of the liquid drop.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A fluid flow device, characterized by: including the assembly takes place for the droplet, flow chamber subassembly, sampling device and nozzle assembly, the assembly takes place for the droplet with sampling device connects, flow chamber subassembly has the first passageway that can supply the liquid stream circulation, sampling device's distal end stretches into first passageway, nozzle assembly set up in the distal end of flow chamber subassembly, flow chamber subassembly is configured to the liquid stream gathering that the sampling device flows out, nozzle assembly has the second passageway and is configured to with the liquid stream forms the efflux, the assembly is taken place for the droplet is configured to with the efflux breaks into the liquid stream.
2. The fluid flow device according to claim 1, wherein: the liquid drop generating assembly comprises at least one piezoelectric ceramic arranged outside the sample feeding device and at least one insulating ceramic connected with the outside of the sample feeding device.
3. The fluid flow device according to claim 2, wherein: the flow chamber assembly comprises a first body and a second body connected with the first body, and the first body and the second body are detachably or integrally connected; the first flow through hole comprises a first flow through hole formed in the first body and a third flow through hole formed in the second body, the first flow through hole is communicated with the third flow through hole, at least one liquid inlet is formed in the first body, and the liquid inlet is communicated with the first flow through hole.
4. The fluid flow device according to claim 3, wherein: the inner diameter of the third through-flow hole is smaller than that of the first through-flow hole.
5. The fluid flow device according to claim 1, wherein: the nozzle assembly comprises a nozzle body, the second passage comprises an overflowing hole and a jet hole which are communicated with each other, and the hole diameter of the overflowing hole is gradually decreased from a near end to a far end.
6. The fluid flow device according to claim 5, wherein: the nozzle assembly also has a seal groove formed in the proximal end of the flowthrough bore for fitting a seal ring.
7. The fluid flow device according to claim 6, wherein: the nozzle assembly is removably attached to a fixture assembly that includes a fixture body having at least one support member configured to apply pressure to and lift the nozzle assembly when in place, causing the nozzle assembly to abut a distal end of a flow chamber assembly when lifted.
8. The fluid flow device according to claim 1, wherein: the liquid flow device also comprises a liquid drop charging device, wherein the liquid drop charging device comprises a power-on end and a grounding end connected with one part of the power-on end, and the other part of the power-on end is connected with a sample feeding device.
9. The fluid flow device according to claim 1, wherein: and the near end of the flow chamber assembly is also connected with a mounting seat, and a joint assembly is rotatably arranged on the mounting seat and is connected with the near end of the sample feeding device so as to rotate the joint assembly to enable the sample feeding device to be fixed by the joint assembly.
10. Analytical sorting equipment, characterized by: the device comprises the liquid flow device of any one of claims 1 to 9 and a sample loading device connected with the liquid flow device, the analysis and sorting equipment further comprises a detection module, a control module and a sorting module, wherein the sample liquid is transferred to the liquid flow device by the sample loading device, the detection module is used for detecting at least one characteristic of biological particles in the sample liquid, the liquid flow device is used for sample liquid focusing, generating a liquid droplet flow and charging liquid droplets, the control module sorts and sorts the liquid droplets according to the at least one characteristic of the biological particles in the liquid droplets, and the sorting module places the liquid droplets into a designated position by utilizing the sorting and sorting of the liquid droplets.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5602039A (en) * | 1994-10-14 | 1997-02-11 | The University Of Washington | Flow cytometer jet monitor system |
| US20110259749A1 (en) * | 2010-04-23 | 2011-10-27 | Bay Bioscience Kabushiki Kaisha | Flow cytometer and flow cytometry |
| CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
| CN211651199U (en) * | 2020-03-12 | 2020-10-09 | 嘉瑞科技（惠州）有限公司 | Magnesium alloy smelting furnace capable of realizing comprehensive liquid leakage detection |
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2021
- 2021-06-11 CN CN202110652066.9A patent/CN113376080A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5602039A (en) * | 1994-10-14 | 1997-02-11 | The University Of Washington | Flow cytometer jet monitor system |
| US20110259749A1 (en) * | 2010-04-23 | 2011-10-27 | Bay Bioscience Kabushiki Kaisha | Flow cytometer and flow cytometry |
| CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
| CN211651199U (en) * | 2020-03-12 | 2020-10-09 | 嘉瑞科技（惠州）有限公司 | Magnesium alloy smelting furnace capable of realizing comprehensive liquid leakage detection |
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