CN106769805B - Cell count sorter - Google Patents
Cell count sorter Download PDFInfo
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- CN106769805B CN106769805B CN201710046115.8A CN201710046115A CN106769805B CN 106769805 B CN106769805 B CN 106769805B CN 201710046115 A CN201710046115 A CN 201710046115A CN 106769805 B CN106769805 B CN 106769805B
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- sheath fluid
- cell count
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- 239000012530 fluid Substances 0.000 claims abstract description 67
- 238000002356 laser light scattering Methods 0.000 claims abstract description 33
- 238000002347 injection Methods 0.000 claims abstract description 14
- 239000007924 injection Substances 0.000 claims abstract description 14
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 6
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 5
- 238000000576 coating method Methods 0.000 claims description 5
- 229910052709 silver Inorganic materials 0.000 claims description 5
- 239000004332 silver Substances 0.000 claims description 5
- 239000004065 semiconductor Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 11
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 78
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000000919 ceramic Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 241000463219 Epitheca Species 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000010224 classification analysis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000004493 neutrocyte Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000000790 scattering method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/12—Coulter-counters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4915—Blood using flow cells
-
- G01N15/01—
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Optical Measuring Cells (AREA)
Abstract
The present invention provides a kind of cell count sorter, including a counter housing, counter housing connects a laser emitter, the top of counter housing is successively arranged jet stream tube socket from top to bottom, gem hole bracket and counting body, the centre of jet stream tube socket is equipped with injection fluid apertures, injection fluid apertures is for injecting cell to be measured, the side for injecting fluid apertures is equipped with interior sheath fluid entrance, the end of gem hole bracket is equipped with outer sheath fluid entrance, the center of gem hole bracket bottom is equipped with the gem hole passed through for cell and interior sheath fluid, body is counted for counting to the cell for flowing through gem hole, the lower section of gem hole is equipped with laser light scattering lens, laser light scattering lens are equipped with a through-hole, outer sheath fluid entrance is inserted into through-hole, the laser issued by collection laser emitter is by the scattered signal after the cell scattering in laser light scattering lens to classify to cell.The present invention can count simultaneously to cell and laser light scattering classification, reduces equipment cost, promotes testing efficiency.
Description
Technical field
The present invention relates to the field of medical instrument technology, more particularly to a kind of cell count sorter.
Background technique
Blood routine examination is clinically one of most common laboratory examination.Blood routine examination project mainly include red blood cell,
Leucocyte, blood platelet, hemoglobin and leukocyte differential count etc., it is physical examination project most basic in clinical examination, significance of which
Be the early indication it can be found that many systemic diseases, diagnosis whether anaemia, if having a disease in the blood system, react marrow
Hematopoiesis function etc..
Wherein leucocyte (white blood cell, abbreviation WBC) is a kind of cell in blood.Leucocyte is also usual
Referred to as immunocyte.It can be seen that, volume is bigger in haemocyte, small number under the microscope.With nucleus.Its
Main function is phagocytosis bacterium, defence disease.Leucocyte is detected by using five sorting techniques at present, i.e., leucocyte is divided into leaching
Bar cell, monocyte, acidophilia, basicyte, neutrophil leucocyte realize the inspection of leucocyte especially by five classifying apparatus
It surveys, five classification can make to detect more acurrate.
With the development with rapid changepl. never-ending changes and improvements of computer technology in recent years, five sorting technique of blood cell analysis is dissipated using laser
It penetrates to detect cell interior structure, by laser irradiation cell, different classes of cell interior grain structure is different, dissipates to laser
It is also just different to penetrate signal.Cell count is then mainly carried out by electrical impedance method.But five classifying apparatus of majority are by cell count at present
Be designed to two components with laser light scattering, detected respectively, cause it is at high cost, apparatus structure dispersion, influence testing efficiency.This
Outside, traditional laser scattering method is when cell passes through flow cell, in laser irradiation to cell, carries out reception cell in low-angle
Scattered signal is easy to be disturbed since laser light scattering signal is weak, it is difficult to obtain accurate laser light scattering signal, only pass through light
Scattered signal is reinforced in electric multiplier tube and fluorescent staining.
Summary of the invention
The present invention is directed at least solve one of the technical problems existing in the prior art.For this purpose, one object of the present invention
Be to propose a kind of cell count sorter, can simultaneously to cell carry out count and laser light scattering classify, reduce equipment at
This, promotes testing efficiency.
A kind of cell count sorter, including a counter housing, the counter housing connect a laser emitter,
The top of the counter housing is successively arranged jet stream tube socket, gem hole bracket from top to bottom and counts body, the jet pipe
The centre of seat is equipped with injection fluid apertures, and the injection fluid apertures is equipped with interior sheath for injecting cell to be measured, the side of the injection fluid apertures
Liquid entrance, the end of the gem hole bracket are equipped with outer sheath fluid entrance, and the center of gem hole bracket bottom is equipped with for thin
The gem hole that born of the same parents and Nei sheath fluid pass through, the counting body is for counting the cell for flowing through the gem hole, the treasured
The lower section in stone hole is equipped with laser light scattering lens, and the laser light scattering lens are equipped with a through-hole, and the outer sheath fluid entrance is inserted into institute
Through-hole is stated, by collecting the laser of the laser emitter sending by the scattering after the cell scattering in the laser light scattering lens
Signal is to classify to cell.
The cell count sorter provided according to the present invention injects cell to be measured, interior sheath fluid by injection fluid apertures first
Cell is wrapped up after the entrance of interior sheath aperture entrance, the cell for having wrapped up interior sheath fluid is passed sequentially through from gem hole, passes through test bag at this time
Counting of the resistance antinoise signal realization of the cell of interior sheath fluid to cell is wrapped up in, the cell counted later enters laser light scattering lens
It is interior, while outer sheath fluid enters laser light scattering lens from outer sheath aperture entrance, the laser quilt issued at this time by collecting laser emitter
The scattered signal after cell scattering in laser light scattering lens, since the scattered signal of different types of cell is different, thus real
Now to the classification of cell, finally realizing and counting and laser light scattering classification are carried out to cell on one device, testing efficiency is high,
The equipment cost in separately test is reduced simultaneously.
In addition, the cell count sorter provided according to the present invention, can also have the following additional technical features:
Further, the laser light scattering lens include the first ellipsoid, and be oppositely arranged with first ellipsoid
Second ellipsoid is equipped with lens face, the laser emitter face institute between first ellipsoid and second ellipsoid
Lens face setting is stated, the outer surface of first ellipsoid is equipped with silver coating.
Further, the cell count sorter further includes scattered signal collector and Fresnel Lenses, described to dissipate
The concave surface setting of the first ellipsoid described in signal collector face is penetrated, the Fresnel Lenses is set to second ellipsoid and institute
It states between scattered signal collector.
Further, the lens face includes at least vertical component effect, and what the vertical component effect and the laser emitter issued swashs
Light is vertical.
Further, the gem hole bracket includes ring part and the tapered portion that connect with the ring part, the interior sheath
Liquid entrance is inserted into the inside of the tapered portion, and the outer sheath fluid entrance is set to the outside of the tapered portion.
Further, the counter housing includes pedestal, and the inside of the pedestal is equipped with accommodating space, and the laser dissipates
Lens are penetrated to set in the accommodating space.
Further, the cell count sorter further includes waste collection pipe, and the waste collection pipe is set to described
The bottom of pedestal, the setting of through-hole described in the waste collection pipe face.
Further, the laser emitter is using semiconductor laser diode as laser source.
Further, the diameter of the gem hole is 70um~80um, including endpoint value.
Further, the diameter of the gem hole is 75um.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for the cell count sorter that one embodiment of the invention provides；
Fig. 2 is the schematic diagram of internal structure of the cell count sorter of Fig. 1；
Fig. 3 is the structural schematic diagram of gem hole bracket in Fig. 1；
Fig. 4 is the structural schematic diagram of laser light scattering lens in Fig. 1.
Main element symbol description:
Cell count sorter 10；Counter housing 11；
Laser emitter 12；Jet stream tube socket 13；
Gem hole bracket 14；Count body 15；
Inject fluid apertures 131；Interior sheath fluid entrance 132；
Outer sheath fluid entrance 141；Gem hole 142；
Ring part 143；Tapered portion 144；
Laser light scattering lens 16；Through-hole 161；
Pedestal 111；Accommodating space 1111；
First ellipsoid 162；Second ellipsoid 163；
Lens face 164；Vertical component effect 1641；
Scattered signal collector 17；Fresnel Lenses 18；
Waste collection pipe 19；
The present invention that the following detailed description will be further explained with reference to the above drawings.
Specific embodiment
To keep objects, features and advantages of the present invention more obvious and easy to understand, with reference to the accompanying drawing to tool of the invention
Body embodiment is described in detail.Several embodiments of the invention are given in attached drawing.But the present invention can be with many not
With form realize, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is makes to this
The disclosure of invention is more thorough and comprehensive.
It should be noted that it can directly on the other element when element is referred to as " being fixedly arranged on " another element
Or there may also be elements placed in the middle.When an element is considered as " connection " another element, it, which can be, is directly connected to
To another element or it may be simultaneously present centering elements.Term as used herein " vertical ", " horizontal ", " left side ",
" right side ", "upper", "lower" and similar statement for illustrative purposes only, rather than the device or member of indication or suggestion meaning
Part must have a particular orientation, be constructed and operated in a specific orientation, therefore be not considered as limiting the invention.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can be machine
Tool connection, is also possible to be electrically connected；It can be directly connected, two members can also be can be indirectly connected through an intermediary
Connection inside part.For the ordinary skill in the art, above-mentioned term can be understood in this hair as the case may be
Concrete meaning in bright.Term " and or " used herein include the arbitrary of one or more relevant listed items and
All combinations.
Referring to FIG. 1, the cell count sorter 10 that one embodiment of the invention provides, including a counter housing 11,
The counter housing 11 connects a laser emitter 12, and the top of the counter housing 11 is successively arranged jet stream from top to bottom
Tube socket 13, gem hole bracket 14 and counting body 15.
Fig. 2 and Fig. 1 is please referred to, the centre of the jet stream tube socket 13 is equipped with injection fluid apertures 131, and the injection fluid apertures 131 is used
In injecting cell to be measured, when it is implemented, the ceramic tube can be passed through by the way that a ceramic tube is arranged in injection fluid apertures 131
Inject cell to be tested.
The side of the injection fluid apertures 131 is equipped with interior sheath fluid entrance 132, and the interior sheath fluid entrance 132 is for injecting interior sheath
Liquid, interior sheath fluid are specifically as follows streaming sheath fluid, and since cell itself is non-conductive, and streaming sheath fluid is a kind of electrolyte solution, can
With conduction, when it is implemented, syringe can be used, then connects hose and inject interior sheath fluid by interior sheath fluid entrance 132, together
When the flow velocity of interior sheath fluid can be controlled by control syringe, it is generally the case that the flow velocity of sheath fluid, which is greater than, in controlling passes through note
The flow velocity of the cell to be measured of liquid shoot hole 131.
The end of the gem hole bracket 14 is equipped with outer sheath fluid entrance 141, and the outer sheath fluid entrance 141 is for injecting epitheca
Liquid, outer sheath fluid is specifically as follows enzyme cleaning solution, for cleaning cell, when it is implemented, syringe also can be used, then connects
Outer sheath fluid will be injected outer sheath fluid by outer sheath fluid entrance 141 by hose, while can also control epitheca by control syringe
The flow velocity of liquid, it is generally the case that the flow velocity of the outer sheath fluid of control is greater than the flow velocity of interior sheath fluid.
Fig. 3 and Fig. 2 is please referred to, the center of 14 bottom of gem hole bracket is equipped with the treasured passed through for cell and interior sheath fluid
Stone hole 142, when it is implemented, it is described injection fluid apertures 131 in ceramic tube and interior sheath fluid entrance 132 in hose face described in treasured
Stone hole 142 is arranged, to guarantee that cell to be measured and interior sheath fluid can enter gem hole 142.The diameter of gem hole 142 be 70um~
80um, including endpoint value, specifically in the present embodiment, the diameter of the gem hole 142 are 75um, the general cell of the numeric ratio
Size it is somewhat larger, therefore the cell that can be just met for having wrapped up interior sheath fluid passes through gem hole 142 one by one, and will not
Cause the case where cell overlap is by gem hole 142.In the present embodiment, the gem hole bracket 14 include ring part 143 and with
The tapered portion 144 that the ring part 143 connects, the interior sheath fluid entrance 132 is inserted into the inside of the tapered portion 144, described outer
Sheath fluid entrance 141 is set to the outside of the tapered portion 144.
The counting body 15 is for counting the cell for flowing through the gem hole 142, when it is implemented, the meter
Number body 15 counts cell using electrical impedance method, and required components are the structure of the prior art, are not done herein superfluous
It states.Cell to be measured can wrap up interior sheath fluid when entering gem hole 142 simultaneously, can guarantee that cell has stable resistance antinoise signal.
When counting, due to extracellularly having wrapped up interior sheath fluid, when cell passes through gem hole 142, resistance antinoise signal can be generated, 1
Signal represents 1 cell, and signal is bigger, represents that cell volume is bigger, to realize the analysis to cell volume.
Fig. 2, Fig. 3 and Fig. 4 are please referred to, the lower section of the gem hole 142 is equipped with laser light scattering lens 16, the laser light scattering
Lens 16 are equipped with a through-hole 161, and the outer sheath fluid entrance 141 is inserted into the through-hole 161, to guarantee that it is sharp that outer sheath fluid is able to enter
In light dispersing lenses 16, by collecting the laser of the sending of laser emitter 12 by the cell in the laser light scattering lens 16
Scattered signal after scattering is to classify to cell.Specifically, the jet stream tube socket 13, gem hole bracket 14, counting body
15 and the through-hole 161 be coaxially disposed.In addition, the counter housing 11 includes pedestal 111, the pedestal in the present embodiment
111 be specially square structure, and the inside of the pedestal 111 is equipped with accommodating space 1111, specifically can be real by way of hollow out
Existing, the laser light scattering lens 16 are set in the accommodating space 1111.
According to cell count sorter provided in this embodiment, cell to be measured is injected by injecting fluid apertures 131 first, it is interior
Sheath fluid wraps up cell after the entrance of interior sheath aperture entrance 132, and the cell for having wrapped up interior sheath fluid is passed sequentially through from gem hole 142, at this time
By testing counting of the resistance antinoise signal realization for having wrapped up the cell of interior sheath fluid to cell, the cell counted later, which enters, to swash
In light dispersing lenses 16, while outer sheath fluid enters laser light scattering lens 16 from outer sheath aperture entrance 141, at this time by collecting laser hair
The laser that emitter 12 issues is by the scattered signal after the cell scattering in laser light scattering lens 16, due to different types of cell
Scattered signal is different, to realize the classification to cell, finally realizes and carries out counting and laser to cell on one device
Scattering classification, testing efficiency is high, while reducing the equipment cost in separately test.
In addition, it is specific in the present embodiment, the laser emitter 12 using semiconductor laser diode as laser source,
Wavelength is about 635nm, can obtain stable light source.
With continued reference to FIG. 4, the laser light scattering lens 16 include the first ellipsoid 162 and with first ellipsoid
162 the second ellipsoids 163 being oppositely arranged are equipped with lens face between first ellipsoid 162 and second ellipsoid 163
164, lens face 164 described in 12 face of laser emitter is arranged, and the outer surface of first ellipsoid 162 is equipped with silver-plated
Layer.The lens face 164 includes at least vertical component effect 1641, the laser that the vertical component effect 1641 is issued with the laser emitter 12
Vertically.
With continued reference to FIG. 2, the cell count sorter 10 further includes that scattered signal collector 17 and Fresnel are saturating
Mirror 18, the concave surface setting of the first ellipsoid 162 described in 17 face of scattered signal collector, the Fresnel Lenses 18 are set to
Between second ellipsoid 163 and the scattered signal collector 17.
Difference carries out receiving cell scattered signal in low-angle with traditional, needs to be arranged multiple laser signal receivers,
In the present embodiment, since the outer surface of the first ellipsoid 162 is equipped with silver coating, the laser that laser emitter 12 issues is dissipated by laser
After penetrating the cell scattering in lens 16, it can all be reflected by the silver coating, the laser then scattered passes through the second ellipsoid 163
Afterwards, it is finally collected by scattered signal collector 17, is used for subsequent analysis, therefore can be realized the extensive angle of laser light scattering signal
It receives, and no longer needs to that multiple laser signal receivers are arranged, it is only necessary to which a laser signal receivers, silver coating can be to sharp
Optical signal is concentrated, and is kept the laser light scattering signal obtained stronger, is no longer needed to reinforce using photomultiplier tube or fluorescent staining
Scattered signal saves cost, can make to detect more acurrate, while avoiding pollution of the fluorescent dye to environment.In addition, logical
Setting Fresnel Lenses 18 is crossed, laser can be assembled, the accuracy of detection is further enhanced.It should be understood that in reality
When border is implemented, the analysis of cell volume and the laser light scattering signal of cell when body 15 counts cell can also will be counted
Combine and classification analysis is carried out to cell, guarantees the accuracy of testing result.
Please refer to Fig. 1 and Fig. 2, in the present embodiment, the cell count sorter 10 further includes waste collection pipe 19, institute
The bottom that waste collection pipe 19 is set to the pedestal 111 is stated, through-hole 161 described in 19 face of waste collection pipe is arranged, described
Waste collection pipe 19 is used to collect the cell that test is completed.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
1. a kind of cell count sorter, including a counter housing, the counter housing connects a laser emitter,
It is characterized in that, the top of the counter housing is successively arranged jet stream tube socket, gem hole bracket from top to bottom and counts body, institute
The centre for stating jet stream tube socket is equipped with injection fluid apertures, and the injection fluid apertures is for injecting cell to be measured, the side of the injection fluid apertures
Equipped with interior sheath fluid entrance, the end of the gem hole bracket is equipped with outer sheath fluid entrance, and the center of gem hole bracket bottom is set
There is the gem hole passed through for cell and interior sheath fluid, the counting body is based on carrying out the cell for flowing through the gem hole
Number, the lower section of the gem hole are equipped with laser light scattering lens, and the laser light scattering lens are equipped with a through-hole, and the outer sheath fluid enters
Mouth is inserted into the through-hole, is scattered by collecting the laser that the laser emitter issues by the cell in the laser light scattering lens
Scattered signal afterwards to classify to cell, the laser light scattering lens include the first ellipsoid, and with first ellipsoid
The second ellipsoid that face is oppositely arranged is equipped with lens face, the laser between first ellipsoid and second ellipsoid
The outer surface of the setting of lens face described in transmitter face, first ellipsoid is equipped with silver coating.
2. cell count sorter according to claim 1, which is characterized in that the cell count sorter also wraps
Scattered signal collector and Fresnel Lenses are included, the concave surface of the first ellipsoid described in the scattered signal collector face is arranged,
The Fresnel Lenses is set between second ellipsoid and the scattered signal collector.
3. cell count sorter according to claim 1, which is characterized in that the lens face includes at least vertical
Portion, the vertical component effect are vertical with the laser that the laser emitter issues.
4. cell count sorter according to claim 1, which is characterized in that the gem hole bracket includes ring part
And the tapered portion connecting with the ring part, the interior sheath fluid entrance are inserted into the inside of the tapered portion, the outer sheath fluid entrance
Set on the outside of the tapered portion.
5. cell count sorter according to claim 1, which is characterized in that the counter housing includes pedestal,
The inside of the pedestal is equipped with accommodating space, and the laser light scattering lens are set in the accommodating space.
6. cell count sorter according to claim 5, which is characterized in that the cell count sorter also wraps
Waste collection pipe is included, the waste collection pipe is set to the bottom of the pedestal, the setting of through-hole described in the waste collection pipe face.
7. cell count sorter according to claim 1, which is characterized in that the laser emitter uses semiconductor
Laser diode is as laser source.
8. cell count sorter according to claim 1, which is characterized in that the diameter of the gem hole be 70um~
80um, including endpoint value.
9. cell count sorter according to claim 8, which is characterized in that the diameter of the gem hole is 75um.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046115.8A CN106769805B (en) | 2017-01-22 | 2017-01-22 | Cell count sorter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046115.8A CN106769805B (en) | 2017-01-22 | 2017-01-22 | Cell count sorter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106769805A CN106769805A (en) | 2017-05-31 |
| CN106769805B true CN106769805B (en) | 2019-06-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710046115.8A Active CN106769805B (en) | 2017-01-22 | 2017-01-22 | Cell count sorter |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3068469B1 (en) * | 2017-06-28 | 2020-09-11 | Diagdev | MEASURING TANK FOR ENUMERATION AND / OR CHARACTERIZATION OF CELLS |
| CN107449716A (en) * | 2017-07-25 | 2017-12-08 | 深圳市孔雀生物科技有限公司 | A kind of sorting unit of flow cytometer |
| CN109490175A (en) * | 2018-10-17 | 2019-03-19 | 南宁优康电子科技有限公司 | Cell count sorter |
| CN216594694U (en) * | 2020-08-07 | 2022-05-24 | 北京鑫骥金诺医疗器械有限公司 | Bacterium counting assembly with sheath flow impedance sensor |
| CN115598024B (en) * | 2022-11-16 | 2023-03-14 | 长春光吉科技有限责任公司 | Cell grading counting device and method thereof |
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