CN101236194A - The sample analyzer and its control system - Google Patents

The sample analyzer and its control system Download PDF

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CN101236194A
CN101236194A CN 200810005238 CN200810005238A CN101236194A CN 101236194 A CN101236194 A CN 101236194A CN 200810005238 CN200810005238 CN 200810005238 CN 200810005238 A CN200810005238 A CN 200810005238A CN 101236194 A CN101236194 A CN 101236194A
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measurement
sample
blood
mode
body fluid
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CN 200810005238
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CN101236194B (en )
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成定宪志
福间大吾
长井孝明
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希森美康株式会社
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Abstract

The invention provides a sample analyzer including measurement device, a measurement sample from a blood sample or a body fluid sample which differs from the blood sample, measures the prepared measurement sample, obtains characteristic information representing characteristics of the components in the measurement sample; mode setting units, sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; first control units, measures the measurement sample prepared from the blood sample by executingoperations in the blood measurement mode when the blood measurement mode has been set; second control units, measures the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set. A control system is also provided.

Description

标本分析仪及其控制系统技术领域:本发明涉及一种不仅能测定血液标本、也能测定脑脊液(髓液)、 胸水(胸膜液)和腹水等血液以外的其他体液的标本分析仪及其控制系统。 TECHNICAL FIELD The specimen analyzer control system: The present invention relates not only to determine blood samples can be measured and a control sample analyzer other body fluids other than cerebrospinal fluid (spinal fluid), pleural fluid (pleural fluid) such as blood and ascites and system. 背景技术:在临床检査领域,作为诊治监视器的辅助手段之一,常用检查装置以采自身体上的血液为被检试样进行测定。 BACKGROUND OF THE INVENTION: In the field of clinical examination, diagnosis and treatment as an adjunct to one monitor, the conventional inspection apparatus is to be measured is collected from a sample of blood on the subject's body. 同时,也用检査装置以血液以外的其他体液为被检试样进行测定。 At the same time, also measured as a test sample inspection apparatus other than the blood of bodily fluids. 通常体液是透明的,其中几乎不含细胞,但当患病或有关器官有肿瘤和损伤时,就会发现出血(血细胞)和异常细胞、细菌等细胞。 Body fluid is usually clear, where almost no cells, but when illness or injury, and cancer-related organs, will find bleeding (blood cells) and abnormal cells, bacteria and other cells. 比如,测定作为体液之一的脑脊液时,从测定结果可能作出如下推断:*红细胞增加:蛛网膜下出血,中性细胞增加:髓膜炎*嗜酸性细胞增加:感染性疾病(寄生虫、真菌) ,单核细胞增加:结核性髓膜炎、病毒性髓膜炎,其他细胞:肿瘤向髓膜进展作为可测定体液中细胞的血细胞分析仪,有第2003-344393号日本专利公开公报上公开的装置。 For example, when measured as one of the cerebrospinal fluid, may be inferred from the measurement result made the following: * increased erythrocyte: subarachnoid hemorrhage, neutrophil increase: * eosinophil meningitis increase: infectious diseases (parasites, fungi ), mononucleosis: tuberculous meningitis, viral meningitis, other cells: tumor progression may be determined as the meninges cells in body fluids blood cell analyzer, there is a Japanese Patent Publication No. 2003-344393 discloses s installation. 据此第2003-344393号日本专利公开公报上记述,为了长期稳定地保存体液,操作人员将体液标本与试剂(醛、 表面活性剂和环糊精)混合,事前制备测定试样,将此测定试样供给分析仪分析体液。 Accordingly No. 2003-344393 described in the Japanese Patent Publication, in order to preserve the body fluid stably for a long, the operator will be a body fluid sample (aldehyde, surfactants and cyclodextrins) mixing with a reagent, sample preparation prior measurement, this measurement a body fluid sample supply analyzer. 然而,据上述第2003-344393号专利公开公报记述,在测定体液时, 不是由分析仪制备测定试样,而是必须由操作分析仪的操作人员来制备测定试样。 However, according to the above-described Patent Publication No. 2003-344393 described in the body fluid measurement, the measurement sample is not prepared by the analyzer, but must be prepared measurement specimen by the operator's operation of the analyzer. 而且,第2003-344393号专利公开公报上没有关于所公开的分析仪测定体液时根据体液进行适当的测定运行的记述。 Moreover, there is no disclosure about the body fluid analyzer described suitable assay run on a body fluid measurement Patent Publication No. 2003-344393 Publication. 发明内容:本发明的范围只由后附权利要求书所规定,在任何程度上都不受这一节发明内容的陈述所限。 SUMMARY OF THE INVENTION: The present invention is defined solely by the scope of the appended claims specified, are not subject to any degree in this section of the statements within the invention. 本发明提供一种标本分析仪,包括:测定装置,从血液标本或不同于所述血液标本的体液标本制备测定试样,测定所制该测定试样, 获取表示所述测定试样中的成份特征的特征信息;模式设定单元,用于设定测定血液标本的血液测定模式和测定体液标本的体液测定模式其中之一作为运行模式;第一控制单元,当所述模式设定单元设定了所述血液测定模式时,控制所述测定装置实施血液标本测定运行;第二控制单元,当所述模式设定单元设定了所述体液测定模式时,控制所述测定装置实施与所述血液标本测定运行不同的体液标本测定运行。 The present invention provides a sample analyzer, comprising: measuring means, the measurement sample from a blood sample or a body fluid sample is different from the prepared measurement sample, measuring the prepared blood samples, sample acquisition component representing the measurement characteristic feature information; mode setting means set the measurement mode for measuring the blood sample and measuring the blood measurement mode in which one of a body fluid as a body fluid specimen operating mode; a first control means, when the mode setting unit when the measurement mode of the blood, said measuring means controlling a blood sample measurement operation embodiment; second control means, when the mode setting unit sets up the body fluid measurement mode, said control means measuring the embodiments different blood sample measurement operation bodily fluid sample measurement operation. 所述的标本分析仪中所述测定血液标本的运行包括所述测定装置在第一时间测定由所述血液标本制备的测定试样的运行;所述测定体液标本的运行包括所述测定装置在与所述第一时间不同的第二时间测定由所述体液标本制备的测定试样的运行。 The operation of the sample analyzer in the measurement means comprises measuring the blood specimen by the measurement operation of the sample prepared from blood samples in a first time of the measurement; the measurement operation of the bodily fluid sample comprises measuring means a second time and the first time measurement operation by the measurement different from the preparation of sample specimens of body fluids. 所述第二时间为比所述第一时间长的时间。 The second time is longer than the first time period. 所述测定血液标本的运行包括所述测定装置制备含所述血液标本和第一数量试剂的测定试样的运行;所述测定体液标本的运行包括所述测定装置制备含所述体液标本和与所述第一数量不同的第二数量的所述试剂的测定试样的运行。 Running the assay blood samples including running measurement sample preparing apparatus comprising a first number of said blood sample and the reagent of the measurement; running the assay sample comprises a body fluid was prepared containing the measurement means and said body fluid sample with determining the number of different runs of the first reagent of a second number of samples. 所述测定血液标本的运行包括所述测定装置制备含所述血液标本和第一试剂的测定试样的运行;所述测定体液标本的运行包括所述测定装置制备含所述体液标本和种类不同于所述第一试剂的第二试剂的测定试样的运行。 Running the assay blood samples including running the measurement sample and a blood sample was prepared containing a first reagent to the measuring apparatus; operating the bodily fluid sample comprises measuring the measurement specimen prepared containing means of said fluid and the different types run measurement sample in the second reagent of the first reagent. 所述的标本分析仪还包括空白检测控制单元,用于控制所述测定装置在从所述体液标本制备所述测定试样前,测定不含所述血液标本和所述体液标本的空白试样。 The sample analyzer further comprises a white detection control unit for controlling the measurement means before the sample blank measurement sample prepared from the body fluid sample, the measurement of the blood sample does not contain the bodily fluid specimen and . 所述的标本分析仪还具备比较单元和清洗控制单元;比较单元用于将对所述测定装置测定由体液标本制备的测定试样所得特征信息进行分析获得的分析结果与所定标准值进行比较;清洗控制单元用于控制所述测定装置在所述比较单元比较认为该分析结果大于所述标准值时实施清洗运行。 The sample analyzer further comprises a comparison unit and a purge control unit; means for comparing said measuring means for measuring will be obtained by the analysis result obtained by measuring the characteristics of bodily fluid specimen sample preparation information is compared with a predetermined reference value; purge control means for controlling said measuring means in said comparison unit compares the analysis result that is greater than the standard value embodiment cleaning operation. 所述的标本分析仪还具备禁止手段,当所述分析结果大于所述标准值时,禁止让所述清洗控制单元控制所述测定装置实施所述清洗运行。 The sample analyzer further comprises means for prohibiting, when the analysis result is greater than the standard value, prohibits the purge control unit controls the measurement apparatus embodiment of the cleaning operation. 所述的标本分析仪中所述体液标本选自包括脑脊液、胸水、腹水、 心囊液、关节液、腹膜透析的透析液和腹腔内清洗液在内的液体。 The specimen analyzer in the bodily fluid sample comprises a liquid selected from the cerebrospinal fluid, pleural effusion, ascites, pericardial fluid, synovial fluid, peritoneal dialysis the dialysate and the cleaning liquid including intraperitoneal. 本发明还提供一种标本分析仪,包括:测定装置,从血液标本或不同于所述血液标本的体液标本制备测定试样,测定所制该测定试样, 获取表示所述测定试样中的成份特征的特征信息;模式设定单元,用于设定测定血液标本的血液测定模式和测定体液标本的体液测定模式其中之一作为运行模式;第一分析单元,当所述模式设定单元设定了所述血液测定模式时,根据所述测定装置测定从所述血液标本制备的测定试样所获取的所述特征信息进行第一分析处理;第二分析单元, 当所述模式设定单元设定了所述体液测定模式时,根据所述测定装置测定从所述体液标本制备的测定试样所获取的所述特征信息迸行不同于所述第一分析处理的第二分析处理。 The present invention also provides a sample analyzer, comprising: measuring means, from blood samples or blood samples is different from the measurement sample prepared specimens of body fluids, measurement of the measurement sample prepared, representing the acquired measurement sample ingredients characteristic feature information; mode setting means for setting a mode of measuring blood measuring blood samples and assays wherein one of the body fluid measurement mode as the operating mode of the specimens of body fluids; first analysis unit, when the mode setting means is provided the timing of the blood measurement mode, the measurement apparatus according to the measured characteristic information of the measurement sample prepared from a blood sample acquired a first analysis process; second analysis unit, when the mode setting unit when the body fluid measurement mode is set, based on the feature acquired from the measuring means measuring the body fluid sample information of the measurement specimen prepared into line different from the second analysis processing of the first analysis process. 所述的标本分析仪中所述第一分析处理包括将白细胞分类为至少四类的处理;所述第二分析处理包括将白细胞分类为少于所述第一分类处理的类别的处理。 The specimen analyzer in the first analysis processing includes processing at least four leukocyte is; and the second analysis processing includes processing leukocyte less than the first category classification process. 所述的标本分析仪中所述第二分析处理将白细胞分类为多核白细胞和单核白细胞。 The specimen analyzer in the second analysis process to classify leukocytes and monocytes polymorphonuclear leukocytes. 所述的标本分析仪中所述第二分析处理包括分析由所述体液标本制备的测定试样中所含一定粒子的处理;所述第一分析处理不包括分析由所述血液标本制备的测定试样中所含所述所定粒子的处理。 The specimen analyzer in the second analysis processing comprises analyzing a measurement sample prepared from the body fluid sample particles contained in a certain process; the first analysis processing by the measurement of the blood analyzing the specimen does not include sample particles contained in the predetermined process. 所述的标本分析仪中所述一定粒子包括选自由巨噬细胞、中皮细胞、肿瘤细胞及其组合构成的群体的粒子。 It said certain of said sample analyzer comprising particles selected from the group consisting of macrophages, mesothelial cells particles, a tumor cell population, and combinations thereof. 本发明进一步提供再一种标本分析仪,包括:测定装置,从血液标本或不同于所述血液标本的体液标本制备测定试样,测定所制该测定试样,获取表示所述测定试样中的成份特征的特征信息;模式切换单元,用于将运行模式从测定血液标本的血液测定模式切换到测定体液标本的体液测定模式;空白检测控制单元,当所述模式切换单元将运行模式从测定血液标本的血液测定模式切换到测定体液标本的体液测定模式时,控制所述测定装置测定既不含所述血液标本也不含所述体液标本的空白试样。 The present invention further provides yet another sample analyzer, comprising: measuring means, said blood specimen from a blood sample or a body fluid sample is different from the prepared measurement sample, the measurement sample prepared by the measurement, the measurement sample acquired represents characterized by the feature information component; mode switching unit for switching the operating mode from the blood measurement of a blood sample of a body fluid measurement mode to a body fluid measurement mode measurement specimen; blank detection control unit, the mode switching unit when the operation mode from the measurement when the blood measurement mode is switched to the blood sample body fluid measurement mode measurement of specimens of body fluids, controlling the measuring means for measuring the blood sample containing neither white nor the body fluid sample specimen. 所述的再一种标本分析仪,还包括:分析单元,通过分析所述测定装置测定由所述体液标本制备的测定试样得出的所述特征信息获取分析结果;判断单元,在所述模式切换单元将运行模式从血液测定模式切换到体液测定模式时测定所述空白试样的基础上,根据所述分析结果判断是否需要测定装置再次测定所述空白试样。 According to still another sample analyzer, further comprising: an analyzing unit acquires the analysis result by analyzing the characteristic information obtained by the measuring means of the body fluid measurement sample prepared by the measurement sample; determining means, the the blank sample is determined on the basis of the mode switching unit switches the operation mode from the blood measurement mode to the body fluid measurement mode, based on the analysis results of the measurement apparatus determines whether the blank sample was measured again. 所述的再一种标本分析仪还包括指示接受单元,当所述判断单元判断测定装置有必要再次测定所述空白试样时,接受开始所述空白试样测定的指示或取消所述空白试样测定的指示。 According to still another sample analyzer further comprises instruction accepting unit, when the determining unit determines a blank test measuring means necessary to determine again the blank sample, to accept an instruction to start the measurement of the sample blank or cancellations It indicates the sample measured. 所述的再一种标本分析仪,还包括:显示器,显示所述分析单元取得的分析结果;显示控制单元,控制所述显示器在所述指示接受单元接到取消所述空白试样测定的指示后,显示分析单元所得分析结果, 以便能够确认所述空白试样测定已取消。 According to still another sample analyzer, further comprising: a display to display the analysis result obtained by said analysis means; display control unit controls the display unit to accept the instruction to cancel the instruction of the measurement of the sample blank after, the resulting display analysis unit analyzes a result, to be able to confirm the measurement sample blank has been canceled. 所述的再一种标本分析仪,还具有第二空白检测控制单元,用于当运行模式设定为所述血液测定模式或运行模式既没设定为所述血液测定模式,也没设定为所述体液测定模式时,控制所述测定装置测定所述空白试样。 According to still another sample analyzer, the blank further comprising a second detection control means for, when the operation mode is set to the measurement of blood or run mode is set to neither the blood measurement mode, it is not set when the body fluid measurement mode, the control means determining the measurement of the sample blank. 所述的再一种标本分析仪还包括第一判断单元和第二判断单元。 According to still another sample analyzer further comprises a first judging unit and a second determination unit. 第一判断单元用于比较根据所述空白检测控制单元的控制所测定的空白试样的第一测定值和第一阀值,判断所述第一测定值是否在容许范围内;第二判断单元用于比较根据所述第二空白检测控制单元的控制所测定的空白试样的第二测定值和第二阀值,判断所述第二测定值是否在容许范围内,所述第一阀值比所述第二阀值小。 A first judging means for comparing the measured value of the sample blank in accordance with a first control unit controlling said space detection and the measurement of the first threshold, determining whether the first measurement value is within an allowable range; second determination means a second measurement value and the second threshold value, determining a second measurement value for the blank sample measured based on the comparison to control the second control unit detects the blank is within the allowable range, the first threshold value smaller than the second threshold value. 本发明同时提供一种标本分析仪的分析控制系统,包括: 一个测定试样制备控制系统,用于控制完成从血液标本或不同于该血液标本的体液标本制备测定试样的步骤; 一个测定试样测定控制系统,用于控制完成测定所制测定试样的步骤; 一个特征信息获取控制系统,用于控制完成获取表示所述测定试样所含成份特征的特征信息的步骤; 一个运行模式设定控制系统,用于控制完成设定测定血液标本的血液测定模式和测定体液标本的体液测定模式其中之一作为运行模式的步骤;及一个测定试样选择测定控制系统,用于完成当设定为所述血液测定模式时,实施血液标本测定运行,测定从所述血液标本制备的测定试样,当设定为所述体液测定模式时,实施与所述血液标本测定运行不同的体液标本测定运行,测定由所述体液标本制备的测定试样的步骤。 The present invention also provides a control system for analysis of the sample analyzer, comprising: a measurement specimen preparation control system, to complete the step of controlling a body fluid measurement sample prepared from blood samples or samples different from that for the blood samples; a measurement sample sample measurement control system for a measurement step of measuring a sample prepared control is completed; a characteristic information acquisition control system for controlling the completion of the step of acquiring characteristic information showing characteristics of the ingredients contained in the sample is measured; one operating mode is provided PID control system, for controlling the completion of blood measurement mode setting assay blood samples and body fluid measurement mode in which one of a body fluid specimen as the operating mode measuring step; a sample selection and measurement control system determines, when the setting for performing when the blood measurement mode, the blood sample measurement operation embodiment, the measurement sample prepared from the blood sample when the body fluid measurement mode is set, a body fluid sample measurement operation of a different embodiment of the same blood sample operation, measured by the measurement step of preparing said sample of bodily fluid specimens. 附图说明:图1为显示本发明一实施方式的标本分析仪的外观图。 BRIEF DESCRIPTION OF DRAWINGS: FIG. 1 is an external view of a sample analyzer of the embodiment of the present invention. 图2为标本分析仪测定装置的框图。 FIG 2 is a block diagram of the sample analyzer apparatus. 图3为流体设备的框图。 FIG 3 is a block diagram of the fluidic device. 图4为白细胞检测器的光学系统显示图。 The optical system 4 is a graph showing the WBC detector. 图5为RBC/PLT检测器的显示图。 FIG 5 is a RBC / PLT detector showing. 图6为HGB检测器的显示图。 FIG 6 is a graph showing the HGB detector. 图7为标本测定处理的流程图。 FIG 7 is a flowchart of the specimen measurement process. 图8为设定测定模式的显示画面的显示图。 FIG 8 is a graph showing the measurement mode setting display screen. 图9为前序列处理的流程图。 FIG 9 is a flowchart of the processing sequence before. 图10为测定由体液制备的DIFF用测定试样的散点图的模式图。 FIG 10 is a DIFF measurement mode FIG prepared from bodily fluid sample by measuring scattergram. 图11为实施方式的标本分析仪测定结果与对照法的测定结果的对比图。 Comparison of the measurement results of FIG. FIG. 11 is a measurement result of the control method of the embodiment of the sample analyzer. 图12为测定由血液制备的DIFF用测定试样的散点图的模式图。 FIG 12 is a view of a measurement mode DIFF scattergram prepared by the blood sample is measured. 图13为血液测定模式的测定结果的显示画面。 13 is a measurement result of the blood measurement mode display screen of FIG. 图14为体液测定模式的测定结果的显示画面。 14 is a measurement result of body fluid measurement mode of the display screen in FIG. 图15为体液测定模式的测定结果的显示画面。 FIG 15 is a measurement result of body fluid measurement mode of the display screen. 图16为体液测定模式的测定结果的显示画面。 16 is a measurement result of body fluid measurement mode of the display screen in FIG. 图17为在体液测定模式下所显示的开始空白检测的确认画面。 FIG 17 is a body fluid measurement mode in the display screen of the confirmation of the start blank check. 具体实施方式:以下根据附图说明本发明的具体实施方式。 DETAILED DESCRIPTION: The following specific embodiments of the present invention are described with reference to the accompanying drawings. 图1所示为标本分析仪1。 1 is a sample analyzer 1 shown in FIG. 此标本分析仪1作为用于血液检查的多项自动标本分析仪,可以对标本容器(采血管)里的血液标本进行测定,获取表示标本中所含血细胞特征的特征信息,并对该特征信息进行分析处理。 This sample analyzer 1 as the number of automatic analyzer for blood test sample, the sample container can (blood collection tube) in the blood samples was measured, showing characteristic information obtaining blood cells contained in the specimen feature, and the feature information analysis and processing. 此标本分析仪1还可以分析体液。 This sample analyzer 1 can also analyze a body fluid. 在本实施方式的标本分析仪,分析对象体液指血液以外的存在于体腔内的体腔液。 In the sample analyzer of the present embodiment, it refers to a body fluid analyte present in the coelomic fluid of the body cavity other than blood. 具体地说指脑脊液(髓液、CSF:脑室和蛛网膜下隙的积液)、胸水(胸膜液、 PE:胸腔积液)、腹水(腹腔积液)、心囊液(心腔积液)、关节液(滑液:关节、滑液囊和腱鞘内的液体)等。 Specifically refers to cerebrospinal fluid (spinal fluid, CSF: ventricles and subarachnoid effusion), pleural fluid (pleural fluid, PE: pleural effusion), ascites (ascites), pericardial fluid (cardiac effusion) , joint fluid (synovial fluid: the joint, synovial capsule and tendon sheath liquid) and the like. 腹膜透析(CAPD)的透析液和腹腔内清洗液等也可作为体液的一种进行分析。 Peritoneal dialysis (of CAPD) and intraperitoneal dialysate cleaning liquid can also be analyzed as a body fluid. 通常这些液体中几乎没有细胞,但当患病或相关器官有肿瘤和受损伤时,就可能含有血细胞、异常细胞和细菌等细胞。 These cells often have little liquid, but related to diseased organs or tumors, and when damaged, it may contain blood cells, abnormal cells and bacteria cells. 比如,以脑脊液为例,从分析结果上可作出如下临床推断。 For example, with cerebrospinal fluid, for example, we can make the following clinical inferred from the analysis results. 比如,如果红细胞增加,则可能是蛛网膜下出血,若中性细胞增加,则可疑为髓膜炎,如果嗜酸性细胞增加, 则可怀疑患感染性疾病(寄生虫和真菌),如果单核细胞增加,则可怀疑为结核性髓膜炎和病毒性髓膜炎,若其他细胞增加,则可怀疑为肿瘤向髓膜转移。 For example, if the increase in red blood cells, it may be subarachnoid hemorrhage, if the increase in neutrophils, meningitis may be suspected, if the increase in eosinophils, monocytes may suffer if the suspected infectious disease (parasitic and fungal), cell increase may be suspected tuberculous meningitis and viral meningitis, increased if other cells, may be suspected tumor metastasis to the meninges. 至于腹水和胸水等,若除血细胞外还含有中皮细胞、 巨噬细胞和肿瘤细胞等有核细胞,就可以作为怀疑癌等疾病的指标, 通过分析这种血细胞以外的有核细胞,可获得这些指标。 As ascites and pleural effusion, if in addition to blood cells also contain the endothelial cells, macrophages and tumor cells and other nucleated cells, it can be used as suspected index cancer and other diseases, nucleated cells by analysis other than that of blood cells, obtained these indicators. 标本分析仪1由可测定作为试样的血液和体液的测定装置2和对测定装置2输出的测定结果进行处理、取得分析结果的数据处理装置3 构成。 Sample analyzer 1 includes a measurement apparatus can measure blood and body fluids as sample 2 and processing the measurement results output from the measuring device, the data processing apparatus 3 is configured to obtain analysis results. 数据处理装置3包括控制器301、显示器302和输入设备303。 The data processing apparatus 3 includes a controller 301, display 302 and input device 303. 在图1中,测定装置2与数据处理装置3各作为一个装置而存在,也可合二为一作为一个装置。 In Figure 1, the measurement device 2 and the data processing apparatus 3 is present as a unit in each, can also be combined as one device. 图2为标本分析仪1的测定装置2的框图。 2 FIG. 2 is a block diagram of the measurement sample analyzer 1 apparatus. 如图2所示,测定装置2包括血细胞检测部分4、对检测部分4的输出信号(模拟信号)进行处理的模拟信号处理器5、微机6、显示,操作部分7和测定血液和体液的装置机械部分8。 As shown, the measurement device 2 includes blood cell detecting section 4, the output signal (analog signal) of the detection section 4 for processing the analog signal processor 5, a microcomputer 6, a display, and means of measuring blood and body fluids operation section 7 8 mechanical parts. 装置机械部分8包含如下流体设备81。 The device part 8 comprising the fluidic device 81. 图3为流体设备81的结构框图。 3 is a block diagram of a fluidic device 81. 如图3所示,流体设备81包含标本吸移嘴18、数个试剂容器11、采样阀12和反应仓13〜17。 3, the apparatus 81 comprises a fluid specimen pipette tip 18, a plurality of reagent containers 11, sampling valve 12 and reaction chamber 13~17. 标本吸移嘴18从标本容器吸移标本,将该标本送往采样阀12。 The sample aspirating pipette tip 18 specimen from the specimen container, the specimen sent to the sampling valve 12. 采样阀12将导入的标本分成一定量的若干等分。 Introducing the specimen sampling valve 12 into an amount of several aliquots. 此分割数因测定模式(各个测定模式)而有所不同,测定红细胞数、白细胞数、血小板数和血红蛋白浓度的CBC模式标本分为三等分。 This number is divided by the measurement mode (measurement mode each) vary, determination of the number of red blood cells, white blood specimens the CBC count, platelet count and hemoglobin concentration divided into three aliquots. 在上述CBC测定模式之上再加白细胞五分类的CBC+DIFF模式,则标本分为四等分。 In the CBC measurement mode coupled on said WBC classification CBC + DIFF mode, the sample is divided into four equal portions. 在CBC+DIFF测定项目上再加测定网织红细胞的CBC+DIFF+RET模式,分为五等分。 In the CBC + DIFF measurement items plus measurement of reticulocytes CBC + DIFF + RET mode, it is divided into five aliquots. 同样,CBC+DIFF模式的测定项目上再加有核红细胞测定项目的CBC+DIFF+NRBC模式也是标本分为五等分。 Similarly, there is coupled a measurement item CBC + DIFF mode, CBC + DIFF + NRBC mode nucleated erythrocytes are measurement items were divided into five aliquots. 在CBC+DIFF+RET测定项目上再加测定有核红细胞的CBC+DIFF+RET+NRBC模式,则分为六等分。 Coupled with the measurement of nucleated red blood cells CBC + DIFF + RET + NRBC mode on the CBC + DIFF + RET measurement item, is divided into six equal. 以上测定模式全是测定血液的血液测定模式。 Above all measured blood measurement mode of the blood measurement mode. 最后,在测定体液的体液测定模式中,标本分为二等分。 Finally, the measurement of a body fluid in a body fluid measurement mode, the sample is divided into bisected. 试剂(稀释液)从试剂容器11导入此采样阀12,所分割的标本等分与试剂一起输送到反应仓13~17和后述HGB检测器43。 Reagent (diluted solution) into the reagent container from this sampling valve 1112, conveying the divided sample aliquot with a reagent to the reaction chamber along with 13 to 17 described later HGB detector 43. 反应仓13 由无图示的定量泵提供采样阀12抽取的一定量的标本(等分)、 一定量稀释液和一定量的染色液,这些标本和试剂经混合,制备出白细胞四分类(DIFF)用的测定试样。 Sampling valve 13 provided by the metering pump 12 (not shown) to extract a certain amount of sample reaction chamber (aliquots), a certain amount of diluent and an amount of stain solution, by mixing these specimens and reagents to prepare a four leukocyte classification (DIFF ) with the measurement sample. 作为稀释液,可适当使用希森美康株式会社提供的试剂"白细胞溶血素STROMATOLYSER-4DL"。 As the diluent, may be suitably used Sysmex Corporation provides reagents "leukocyte hemolysin STROMATOLYSER-4DL". 此试剂含有表面活性剂,可以溶解红细胞。 This reagent contains surface active agent, to lyse erythrocytes. 染色液同样可适当使用希森美康株式会社提供的试剂"白细胞四分类液STROMATOLYSER-4DS"。 Staining solution may be appropriate to use the same reagents Sysmex Corporation to provide the "four leukocyte classification was STROMATOLYSER-4DS". 此染色液含有乙二醇、低醇、聚甲炔,在上述稀释液溶血后,血细胞成份被染色,最终制作出50倍稀释试样。 This staining solution containing ethylene glycol, lower alcohols, polymethine, after said dilution hemolysis, the blood cell components are stained, to produce the final 50-fold diluted sample. 如果选择体液测定模式的话,则以与此白细胞四分类用测定试样同一标本量、同样试剂和同一试剂量的条件制备白细胞分类用测定试样。 If the body fluid measurement mode is selected - then the same sample amount of the sample was measured using the same reagents and conditions for preparing the same amounts of reagents for leukocyte classification measurement sample classification of leukocytes into four groups with this. 但是,如后所述,体液测定模式的白细胞分类白细胞不是四类, 而是二类。 However, as described later, the body fluid measurement mode leukocyte white blood cells is not four, but two types. 反应仓14由无图示的定量泵提供采样阀12采集的一定量标本、 一定量稀释溶血剂和一定量染色液,将这些标本和试剂混合,制作有核红细胞(NRBC)测定用测定试样。 Reaction chamber 14 by a sampling valve provided a metering pump 12 (not shown) an amount of collected specimen, a hemolytic agent and a dilute amount of a certain amount of staining solution, and those samples reagent mixing, nucleated red blood cell production (the NRBC) was determined by measurement sample . 反应仓15由无图示的定量泵提供采样阀12采集的一定量标本、 一定量稀释溶血剂和一定量染色液,将这些标本和试剂混合,制作网织红细胞(RET)测定用测定试样。 Reaction chamber 15 by a sampling valve provided a metering pump 12 (not shown) an amount of collected specimen, a hemolytic agent and a dilute amount of a certain amount of staining solution, and those samples reagent mixing, production of reticulocytes (RET) assay measurement sample . 反应仓16由无图示的定量泵提供采样阀12采集的一定量标本和一定量稀释溶血剂,将这些标本和试剂混合,制作白细胞和嗜碱性细胞(WBC/BASO)测定用测定试样。 Reaction chamber 16 by a valve (not shown) provide samples of a metering pump 12 and the specimen collected amount amount of dilute hemolytic agent, mixing these specimens and reagents, the production of white blood cells and basophils (WBC / BASO) measurement sample measurement . 反应仓17由无图示的定量泵提供采样阀12采集的一定量标本、一定量稀释液,将这些标本和试剂混合,制作红细胞/血小板(RET/PLT) 测定用测定试样。 Reaction chamber 17 by a metering pump (not shown) an amount of specimen collection sampling valve 12, a certain amount of diluent, the mixing of these specimens and reagents, the production of red blood cells / platelets (RET / PLT) measuring a measurement sample. 另外,采样阀12采集的一定量标本、 一定量稀释溶血剂还供应后述HGB检测器43。 Further, a certain amount of specimen collection sampling valve 12, an amount of dilute hemolytic agent supplied after further HGB detector 43 described later. 检测部分4有检测白细胞的白细胞检测器41 。 4 detecting section for detecting WBC detector 41 leukocytes. 此白细胞检测器41 也用于有核红细胞和网织红细胞的检测。 This detector 41 also for leukocyte nucleated erythrocytes and reticulocytes detected. 检测部分4除白细胞检测器41夕卜,还有测定红细胞数和血小板数的RBC/PLT检测器42和测定血液中血色素量的HGB检测器43。 Detection section 4 leukocyte Bu Xi detector 41, and measuring the number of red blood cells RBC and platelet counts / PLT detector 42 and the measurement of hemoglobin content in blood HGB detector 43. 上述白细胞检测器41主要由光学检测器构成,具体而言,由运用流式细胞术的检测器构成。 WBC detector 41 described above is mainly constituted by an optical detector, in particular, constituted by a detector using flow cytometry. 在此,所谓细胞术即测定细胞和其他生物学粒子的物理性质和化学性质,所谓流式细胞术指:让这些粒子从细流中通过、进行测定的方法。 Here, i.e. cytometry assay physical and chemical properties of cells and other biological particles, refers to the so-called flow cytometry: let these particles pass through the fine flow, measurement method. 图4所示为白细胞检测器41的光学系统。 Figure 4 is a WBC detector 41 of the optical system. 在该图中,从激光发光二极管401射出的光束通过准直镜402照射到流经鞘流池403内的血细胞。 In this figure, the laser beam emitted from the light emitting diode 401 through the collimator lens 402 is irradiated to blood cells flowing within the sheath flow cell 403. 此白细胞检测器41检测鞘流池403内的血细胞在激光照射下所发出的前向散射光强度、侧向散射光强度和侧向荧光强度,以此作为血细胞特征参数。 Before leukocyte blood cells within this detector 41 detects the sheath flow cell 403 is irradiated with the laser emitted forward scattered light intensity, side scattered light intensity and side fluorescence intensity, as characteristic parameters of blood cells. 在此,光的散射是由于血细胞这种粒子在光线的行进方向上成为障碍物,光因此改变其行进方向所产生的现象。 Here, scattered light due to the blood cells such particles become an obstacle in the traveling direction of the light, thus changing the light traveling direction phenomenon generated. 检测这种散射光可以获得有关粒子大小和成份的粒子特征信息。 Detecting the scattered light can be obtained information about the particle characteristics of particle size and composition. 前向散射光是指粒子发出的与所照射光线的行进方向基本相同的散射光。 Substantially identical front light refers to the direction of travel of the particles emitted by the light irradiation scattered light scattering. 从前向散射光可以获得有关粒子(血细胞)大小的特征信息。 Characteristic information can be obtained from the size (blood cell) particles to scatter light. 侧向散射光是粒子发出的与所照射光线略呈垂直方向的散射光。 Side scatter light and the scattered light rays irradiated in the vertical direction slightly emitted by the particle. 从侧向散射光可以获得有关粒子内部的特征信息。 It can be obtained from the side scattered light information about internal particle characteristics. 当激光照射到血细胞粒子上时,侧向散射光强度取决于细胞内部的复杂性(核的形状、大小、密度和颗粒的数量)。 When a laser is irradiated to the blood cell particle, the lateral scattered light intensity depends on the complexity of the cell interior (the number of shape, size, density and particle nuclei). 因此,利用侧向散射光强度的这一特性可以分类(鉴别)血细胞,并测定血细胞数量。 Thus, the use of side-scattered light intensity This feature classification (discrimination) of blood cells, and measuring the number of blood cells. 另外,已述本实施方式采取了使用前向散射光和侧向散射光作为散射光的结构,但不限于此,只要是能够获得分析所需要的反映粒子特征的散射光信号,散射光对于光源透过鞘流池照射的光的光轴的角度不限。 Further, the present embodiment has been described taking a configuration using the forward scattered light and side-scattered light as scattered light, but is not limited thereto, as long as it is possible to obtain scattered light signal reflects the particle characteristics required for the analysis, the scattered light to the light source Any light transmitted through the optical axis of the sheath flow cell irradiated angle. 光照诸如经染色的血细胞那种荧光物质,则发出比所照光波波长更长的光。 Blood cells such as illumination by a fluorescent substance that staining is issued is longer than the wavelength of the light as light. 荧光强度是染色越好越强,测定此荧光强度就可获得有关血细胞染色程度的特征信息。 The fluorescence intensity is stronger staining better, this measured fluorescence intensity can be obtained about the degree of staining of the blood cell feature information. 因此,根据(侧向)荧光强度的差,可以对白细胞进行分类等测定。 Thus, according to the difference (side) fluorescence intensity can be measured for leukocyte classification. 如图4所示,流经鞘流池403的血细胞(白细胞和有核红细胞) 发出的前向散射光通过聚光镜404和针孔405被发光二极管(前向散射光集光器)406接受。 As shown, the (forward scattered light concentrators) before issuing 4 406 accepts the blood cells flowing through the sheath flow cell 403 (leukocytes and nucleated red blood cells) the forward scattered light through a collective lens 404 and pinhole 405 are light emitting diodes. 侧向散射光通过聚光镜407、分色镜408、光学膜409和针孔410被光电倍增管(侧向散射光集光器)411接受。 Side scattered light by a condenser lens 407, dichroic mirror 408, the optical film 409 and the pinhole 410 is photomultiplier tube (side scattered light collector) 411 accepts. 侧向荧光通过聚光镜407和分色镜408被光电倍增管(侧向荧光集光器) 412接受。 Fluorescence condenser lens 407 side by the dichroic mirror 408 and photomultiplier tube (side fluorescent light receiver) 412 to accept. 从各集光器406、 411和412输出的所受光信号分别通过由、放大器51、 52、 53等构成的模拟信号处理器5进行放大和波形处理等模拟信号处理后,输送到微机6。 After each of the light collector 406, suffered optical signals 411 and 412 respectively output by the amplifiers 51, 52, 53 and the like of the analog signal processor 5 is amplified and waveform processing analog signal processing, fed to the microcomputer 6. 下面,就RBC/PLT检测器42的结构进行说明。 Hereinafter, a structure of an RBC / PLT detector 42. 图5为RBC/PLT 检测器42的简要结构模式图。 FIG 5 is a RBC / PLT detector 42 a schematic diagram showing the structure of. RBC/PLT检测器42可运用鞘流DC检测法测定红细胞数和血小板数。 RBC / PLT detector 42 can determine the number of red blood cells and platelets using sheath flow DC detection method Number. RBC/PLT检测器42有如图5所示鞘流池42a。 RBC / PLT detector 42 has a sheath flow cell 42a as shown in FIG. 5. 此鞘流池42a设有向上开口的加样口42b,试样可以从反应仓17加入此加样口42b。 This sheath flow cell 42a is provided with upwardly open loading port 42b, the sample may be added to this loading port 42b from the reaction chamber 17. 鞘流池42a还有向上逐渐变细的锥状样仓42c, 上述加样口42b就配置在此样仓42c的内部中央。 The sheath flow cell 42a as well as upwardly tapering cone-like cartridge 42c, above the loading port 42b disposed on the center of the inside of this kind of cartridge 42c. 样仓42c上端设有孔42d,此孔42d正好与加样口42b中心相对。 42c comp cartridge upper end provided with a hole 42d, the hole 42b and 42d diametrically opposite the center of the loading port. 供样器提供的测定试样从加样口42b的前端向上运送,与此同时,前鞘液供应到样仓42c,前鞘液向上流动流向孔42d。 For the measurement sample transported from the sample provided by the front end of the loading port 42b upwardly at the same time, the front sheath liquid sample supplied to the cartridge 42c, the front sheath fluid flows upward hole 42d. 在此,测定试样在前鞘液的包围下流动,锥状样仓42c使测定试样流变细,测定试样中的血细胞逐个通过孔42d。 Here, the measurement sample surrounded by sheath fluid flow front, cone-like cartridge 42c fine rheological measurement sample, blood cell sample was measured one by one through the aperture 42d. 孔42d设电极,此电极间供有直流电流。 Hole electrode 42d provided for this direct current between the electrodes. 检测当测定试样流过孔42d时孔42d的直流电阻的变化,将此电信号输出到控制器25。 When detecting changes in the measurement sample flowing through 42d when the DC resistance Kong and Kong 42d, and outputs this electric signal to the controller 25. 上述直流电阻在血细胞通过孔42d时会增大,因此,这一电信号反映血细胞通过孔42d的信息,通过对这一电信号进行信号处理,对红细胞和血小板计数。 The DC resistance increases when the blood cells through the aperture 42d, and therefore, the electrical signal reflects information hole 42d blood cells by performing signal processing on the electrical signals of red blood cell and platelet counts. 孔42d的上方设有向上下延伸的回收管42e。 Hole 42d is provided above the recovery pipe extending vertically 42e. 此回收管42e配置于通过孔42d与样仓42c相连的样仓42f内。 This recovery tube 42e is disposed within the sample cartridge through the hole 42d and the like connected to the cartridge 42c 42f. 回收管42e下端与样仓42f 内壁分离。 Recovery tube 42e and the lower end of the inner wall of the sample cartridge 42f separated. 样仓42f有后鞘液提供,此后鞘液沿样仓42f的回收管42e 的外侧区域向下流动。 Comp cartridge 42f provided a rear sheath liquid, after sheath fluid along the outer region of the magazine-like recovery pipe 42e 42f flows downward. 从回收管42e外侧流过的后鞘液到达样仓42f 下端后,通过回收管42e下端和样仓42f的内壁之间,流向回收管42e 内部。 After flowing from the outer side of the recovery tube 42e sheath fluid sample reaches the lower end of the cartridge 42f, through the recovery tube 42e and lower end 42f of the inner wall of the cartridge-like, internal flow recovery pipe 42e. 因此可以防止通过孔42d的血细胞回流,从而防止血细胞的误检。 Hole 42d can be prevented by the reflux of blood cells, thereby preventing erroneous detection of blood cells. 下面就HGB检测器43的结构进行说明。 Here will be described the structure of the HGB detector 43. HGB检测器43可通过SLS血红蛋白法测定血色素量(HGB)。 HGB detector 43 can measure the amount of hemoglobin (HGB) by a SLS-hemoglobin method. 图6为HGB检测器43结构的斜视图。 FIG 6 is a perspective view of the structure of FIG. 43 HGB detector. HGB检测器43有装稀释试样的样池43a、向样池43a发光的发光二极管43b和接收透过样池43a的透射光的集光元件43c。 HGB detector 43 mounted diluted sample with a sample cell 43a, 43a for emitting light toward the sample cell and the current light-emitting diodes 43b light receiving element 43a through sample cell of the transmitted light 43c. 采样阀12定量的血液按一定稀释率被稀释液和一定溶血剂稀释,制备稀释试样。 Quantitative sampling valve 12 diluted blood was diluted hemolytic agent and a certain constant dilution rate was prepared diluted sample. 此溶血剂具有将血液中的血红蛋白转换为SLS-血红蛋白的性质。 This hemolytic agent having converting hemoglobin in the blood hemoglobin SLS- properties. 这种稀释试样供向样池43a,存放于样池43a。 This diluted sample supplied to the sample cell 43a, deposited in the sample cell 43a. 在此状态下,使发光二极管43b发光,透射光由隔样池43a与发光二极管43b相对配置的集光元件43c接收。 In this state, the light emitting diode 43b emits light, a light collecting element transmits light like cell compartments 43a and 43b disposed opposite the light emitting diode 43c received. 发光二极管43b所发波长光易于被SLS-血红蛋白吸收,且样池43a由透光性高的塑料材料制成,因此,集光元件43c接收的是发光二极管43b发射光仅被稀释试样吸收后的透射光。 Issued emitting diodes 43b light wavelength easily absorbed SLS- hemoglobin, and the like cell 43a made of a highly light-transmitting plastic material, and therefore, the light-receiving element 43c is a light emitting diode 43b emits light only absorbed by the specimen is diluted the transmitted light. 集光元件43c将与集光量(吸光度)相应的电信号输往微机6,微机6将此吸光度与预先测定的仅稀释液的吸光度比较,算出血红蛋白值。 The light collecting element 43c and the light collecting quantity (absorbance) 6 corresponding electrical signal exported to the microcomputer, the microcomputer 6 is compared with the absorbance of this diluent only absorbance measured in advance, calculates the hemoglobin value. 微机6具有将模拟信号处理器5提供的模拟信号转换为数字信号的A/D转换器61 。 The microcomputer 6 converts the analog signals having analog signal processor 5 provided for the digital signal A / D converter 61. A/D转换器61的输出值输送到微机6的演算器62, 在演算器62进行演算,对集光信号迸行一定的处理。 A / D converter 61 is supplied to an output value calculation 6 of the microcomputer 62, calculation performed in the calculation device 62, an optical signal into line collection of some processing. 演算器62根据检测部分4的输出值制作分布数据(二维散点图(未分类)和一维直方图)。 The arithmetic unit 62 detects the output value of the distribution data production section 4 (two-dimensional scattergram (Unclassified) and a one-dimensional histogram). 微机6包括由控制用处理器和控制用处理器运行用存储器构成的控制器63和由分析用处理器和分析用处理器运行用的存储器构成的数据分析单元64。 6 includes a microprocessor controller 63 with a control processor configured with a memory operation and a data analysis by the analysis unit is configured by a memory and a processor with a processor running the analysis and control processor 64. 控制器63用于控制由自动供应采血管的供样器(图示省略)、制样和测定用的流体系统等组成的装置机械部分8和其他部分。 Controller 63 for controlling sampler for automatically supplied by the blood collection tube (not shown), the device consisting of sample preparation and measurement of the mechanical system, the fluid portion 8 and other parts. 数据分析单元64用于对各分布数据进行筛查等分析处理。 Data analysis unit 64 for each of the distribution data analysis and processing screenings. 分析结果通过接口65输送到外部的数据处理装置3,进行显示数据画面和存储数据等处理。 The results delivered through the interface 65 to the external data processing device 3, the screen display data and processed data and the like stored. 微机6有与显示,操作部分7连接的接口66和与装置机械部分8连接的接口67。 6 has a microcomputer and a display, an operation portion 7 is connected to the interface 66 and the interface 8 is connected with the mechanical portion of the device 67. 演算器62、控制器63和接口66、 67通过总线68连接,控制器63和数据分析单元64通过总线69连接。 Computing section 62, interface 66 and controller 63, 67 is connected by a bus 68, a controller 63 and a data analysis unit 64 is connected by a bus 69. 显示,操作部分7上包括操作人员下达开始测定指示的开始开关和显示仪器状态、各种设定值以及分析结果、接受操作人员输入的触摸屏式液晶显示器。 Display, the operation part 7 comprises operator released the measurement start switch and starts the display instrument status indication, various setting values ​​and analysis results, the liquid crystal display touch screen to accept operator input. 下面就本实施方式的标本分析仪l的运行进行说明。 It will now be described operation of the sample analyzer of the present embodiment l. 图7为本实施方式的标本分析仪运行的流程图。 A flowchart of operation of the sample analyzer of the embodiment of FIG. 7 of the present embodiment. 用户(操作人员)接通标本分析仪l 的电源(步骤S1),启动标本分析仪l。 A user (the operator) turns l sample analyzer of the power (step S1), starting sample analyzer l. 此标本分析仪l启动时先进行自检(步骤S2)。 First self-test (Step S2) When this sample analyzer l promoter. 在自检中,不仅测试微机6、检査标本分析仪l的各运行机械部分的运行情况,还进行测定不含标本的空白试样的空白检测。 In the self-test, the only test the microcomputer 6, the mechanical operation of each operation portion of the specimen analyzer l, further blank check measurement of the sample blank containing no specimen. 然后,微机6对测定模式进行初始设定(步骤S3)。 Then, the microcomputer 6 initially set measurement mode (step S3). 此初始设定值为CBC+DIFF模式。 This initial setting is CBC + DIFF mode. 具体而言,在步骤S3的处理中,设定测定血液的参数(运行条件)、如所用反应仓和测定时间设定等。 Specifically, in the processing in step S3, setting parameters (operating conditions) Measurement of blood, as the reaction vessel and the like by the measurement time setting. 如此,本实施方式的标本分析仪以血液测定模式为初始运行模式。 Thus, the sample analyzer of the present embodiment in the blood measurement mode as the initial operation mode. 据此,标本分析仪l 处于可接受开始测定的待机状态。 Accordingly, the sample analyzer l acceptable in the standby state to start the measurement. 微机6在液晶显示器上显示通知待机状态的画面(步骤S4)。 Computer 6 notifies the standby screen (step S4) displayed on the liquid crystal display. 在此待机状态下,操作人员可通过操作显示'操作部分7切换测定模式。 In this standby state, the operator can display the operation 'operating portion 7 is switched to measurement mode. 图8为设定测定模式的输入画面模式图。 FIG 8 is a measurement mode setting input screen mode in FIG. 此画面中有标本号120、 标本放入模式种类121、分项检测(测定模式)种类122和标本种类123 各个显示画面。 This screen has a specimen number 120, 121 mode type is placed in the specimen, the detection sub-item (measurement mode) 122 and type 123 each specimen type display screen. 标本放入模式设有三种模式:操作人员手动将标本容器插入标本吸移嘴18进行标本吸移的手动模式;操作人员事先将标本与试剂混合制备测定试样、用标本吸移嘴18吸移该测定试样的微量血预稀释模式;由自动运送标本容器的运送装置提供标本的封闭模式。 Mode with the specimen into three modes: the operator manually inserting the sample container specimen pipette tip 18 is aspirated sample manual mode; operator prior specimen measurement sample prepared by mixing with a reagent, a sample suction nozzle 18 move aspirating micro blood pre-dilution mode of the measurement sample; specimens by providing a closed conveying means for automatically conveying the sample container. 作为标本种类,设有普通血液标本常规(Normal)、造血祖细胞用标本HPC和体液(Body Fluid)。 As a specimen type, with conventional normal blood samples (Normal), hematopoietic progenitor cells and body fluid samples HPC (Body Fluid). 操作人员可分别指定标本放入模式、测定模式和标本种类。 The operator can specify the sample into the pattern, the type of specimen and measurement mode, respectively. 操作人员如果指定血液测定模式,则将标本种类指定为常规(Normal),再指定任意标本放入模式和测定模式。 If the operator specifies a blood measurement mode, it will be designated as a conventional type specimen (Normal), then specify any specimen placed mode and measurement mode. 如果指定体液测定模式,则操作人员分别在放入模式中指定"手动模式", 在分项检测中指定"CBC+DIFF" 、 "CBC+DIFF+RET"、 "CBC+DIFF+NRBC"和"CBC+DIFF+NRBC+RET"中之一,在标本种类中指定"体液"。 If the specified body fluid measurement mode, the operating personnel are placed in the specified mode "manual mode", designated "CBC + DIFF" in the sub-detection, "CBC + DIFF + RET", "CBC + DIFF + NRBC" and " CBC + DIFF + NRBC + RET "one, specify the type of the specimen in the" body fluid. " 在步骤S4中,操作人员如此指定所希望的测定模式。 In step S4, so the operator designates a desired measurement mode. 操作人员如果不改变初始设定的测定模式而进行血液测定的话(在步骤S5选择N),则按开始开关下达开始测定指示。 If the operator does not change the measurement mode is initially set, then the blood was measured (at step S5 selection N), press the start switch issued a measurement start instruction. 微机6接收开始测定指示(步骤S6),从标本吸移嘴吸移血液标本(步骤S7)。 The microcomputer 6 receives a measurement start instruction (step S6), the sample from the pipette tip to aspirate the blood sample (step S7). 血液标本吸移后,如上所述标本被导入采样阀12,根据测定模式的分项检测种类调制测定所需要的试样(步骤S14)。 Aspirating the blood sample, the sample is introduced into the sampling valve 12 as described above, the detection sub-type modulation mode is determined according to the desired measurement sample (step S14). 然后实施测定试样的测定运行(步骤S16)。 Then embodiment of the measurement sample measurement operation (step S16). 比如,分项检测种类设定为"7"时,制备HGB、 WBC/BASO、 DIFF、 RET、 NRBC、 RBC/PLT用的各种测定试样。 For example, sub-type is set to detect when a variety of "7", prepared HGB, WBC / BASO, DIFF, RET, NRBC, RBC / PLT measurement sample used. 然后由白细胞检测器41对WBC/BASO、 DIFF、 RET、 NRBC用测定试样进行测定,由RBC/PLT检测器42对RBC/PLT用测定试样进行测定,由HGB检测器43对HGB用测定试样进行测定。 Then the detector 41 pairs of white blood cell counts WBC / BASO, DIFF, RET, NRBC with the measurement sample, the measuring sample was measured by the RBC / PLT detector 42 pairs RBC / PLT, HGB measured by the HGB detector 43 pairs the sample was measured. 此时,白细胞检测器41仅设置有一个,因此,NRBC、 WBC/BASO、 DIFF、 RET各测定试样按NRBC、 WBC/BASO、 DIFF、 RET的顺序依次导入白细胞检测器41,逐项测定。 In this case, leukocytes detector 41 is provided with only one, therefore, NRBC, WBC / BASO, DIFF, RET measurement sample by each of NRBC, WBC / BASO, DIFF, RET sequence introduced into the white blood cell detector 41 sequentially, one by one measurement. 在这一测定运行中,演算器62绘制粒子分布图(散点图、直方图)。 In this measurement operation, the calculation unit 62 plotted particle distribution map (scattergram, histogram). 在此,就根据DIFF测定所得光学信息绘制散点图的过程进行说明。 Here, there will be described the optical information drawing process scattergram obtained DIFF measurement according. 演算器62以在DIFF测定中由白细胞检测器41输出的集光信号中的侧向散射光和侧向荧光信号为特征参数,绘制二维散点图(粒子分布图)。 Computing section 62 of the side scattered light and side fluorescence signal set in the optical signal output by the DIFF measurement detector 41 in leukocytes is characteristic parameters, drawing two-dimensional scattergram (particle distribution map). 此散点图(以下称DIFF散点图)是以侧向散射光强度为X轴、以侧向荧光强度为Y轴绘制的, 一般出现"红细胞血影粒子群"、"淋巴粒子群"、"单核粒子群"、"嗜中性+嗜碱性粒子群"和"嗜酸性粒子群"。 This scattergram (hereinafter referred to as the DIFF scattergram) is a side scattered light intensity on the X axis to the Y-axis side fluorescence intensity is plotted, typically a "erythrocyte ghosts particle group", "lymphatic particle group", "single core particle group", "particle group neutrophils + basophils" and "eosinophils particle group." 这些粒子群由数据分析单元64通过处理DIFF散点图来识别。 These particle group 64 identified by the data analysis unit by processing the DIFF scattergram. 然后,根据测定所得粒子分布图进行分析处理(步骤S18)。 Then, FIG analysis process (step S18) The resulting measured particle distribution. 在此分析处理中,微机6的数据分析单元64对白细胞检测器41测定DIFF测定试样时演算器62绘制的DIFF散点图分类为:图12所示四个白细胞群(淋巴细胞群、单核细胞群、嗜中性+嗜碱性细胞群和嗜酸性细胞群)和红细胞血影群。 In this analysis process, the microcomputer 41 measures the data analysis unit 64 6 leukocytes DIFF measurement sample detector 62 calculating the DIFF scattergram drawn classified as: Figure 12 shows four leukocyte populations (lymphocytes, mono monocyte population, neutrophil + basophil cluster, and eosinophil cluster), and erythrocyte ghosts group. 在本实施方式的分析处理中,从散点图上划分的各粒子与各群的重心位置之间的距离可以得到各粒子对各群的归属度。 In the analysis process according to the present embodiment, each particle is divided from the plot and the distance between the centroid position of each group can be obtained the degree of attribution of each group of each particle. 根据这些归属度各粒子被分为各群。 The attribution of these particles are divided into the respective groups. 这种粒子分类方法在美国专利第5, 555, 196号公报上有详细记载。 This particle classification methods are described in detail in U.S. Patent Publication No. 5, 555, 196. 在WBC/BASO测定所得散点图上,分类为嗜碱性细胞群、嗜碱性细胞以外白细胞群和红细胞血影群。 The resulting measured WBC / BASO scattergram, the basophils are classified into groups, other than the basophilic leukocytes and erythrocyte ghosts population group. 再根据DIFF散点图分析处理对白细胞四分类和计数的结果(参照图12)和WBC/BASO散点图分析处理对白细胞二分类和计数的结果,对血液标本所含白细胞进行五分类。 Then according to the results of the DIFF scattergram analysis of leukocyte classification and counting of four (refer to FIG. 12) and the WBC / BASO scattergram results of analysis and classification of the white blood cell count of two, five-classify leukocytes contained in the blood sample. 具体而言,数据分析单元64从DIFF散点图分析处理所得"嗜中性细胞+嗜碱性细胞的血细胞数"减去WBC/BASO 散点图分析处理所得"嗜碱性细胞的血细胞数",即可分别得出嗜中性细胞的血细胞数和嗜碱性细胞的血细胞数。 Specifically, the data analysis unit 64 analyzing process resulting from the DIFF scattergram "+ the number of blood cells neutrophils basophils" subtracting "the number of blood cells, basophils" WBC / BASO scattergram analysis processing resulting , to count the number of blood cells and blood basophils neutrophils cells were derived. 据此,白细胞被五分类(淋巴细胞、单核细胞、嗜中性细胞、嗜碱性细胞和嗜酸性细胞),获得各项的血细胞数。 Accordingly, white blood cells are classified five (lymphocytes, monocytes, neutrophils, eosinophils and basophils), to obtain the number of blood cells. 此外,在RBC/PLT测定中,检测根据检测器42测得的特征信息绘制的一维直方图的曲线谷值,对红细胞和血小板分类。 Further, the RBC / PLT measurement in accordance with the detection curve valley detector 42 measured characteristic information drawing a one-dimensional histogram, classification of erythrocytes and platelets. 如此取得的分析结果输出到数据处理装置3的显示器302上(步骤S20)。 It outputs the analysis result thus obtained to the data processing device 302 on the display 3 (step S20). 另一方面,微机6如果在步骤S5如上所述接收到指定测定模式为体液测定模式的输入时,设定进行体液测定的参数(运行条件)如所用反应仓、测定时间设定等(步骤S8)。 On the other hand, if the microcomputer 6 at step S5, the received measurement mode is designated as described above to input a body fluid measurement mode, setting the parameters of the measured fluid (operating condition) used as the reaction chamber, the measurement time setting and the like (step S8 ). 在本实施方式中,测定时间如后所述为血液测定时的三倍。 In the present embodiment, the measured tripled after the measurement time, such as blood. 测定模式由其他测定模式(在此为血液测定模式)切换为体液测定模式时(步骤S9),测定装置2开始前序列处理(步骤S10)。 When the measurement mode (in this case the blood measurement mode) to the other body fluid measurement mode to the measurement mode (step S9), the measurement device 2 starts the processing sequence (step S10) before. 此前序列处理是为体液测定做准备。 Prior to the processing sequence is to prepare for a body fluid assay. 体液测定模式下测定的是血细胞成份浓度低的标本,因此,从血液测定模式(在图8中显示为"1:常规") 切换设定为体液测定模式时要进行前序列处理,以确保本底不会影响到体液测定结果。 Measured in a body fluid measurement mode is the low blood cell concentrations of the components of the sample, therefore, from the blood measurement mode (shown in FIG. 8, "1: General") switch is set to pre sequence processing the body fluid measurement mode, to ensure that the substrate does not affect the measurement result of body fluids. 前序列处理包括空白检测。 Presequence including blank check process. 在此前序列处理中的空白检测判断标准比血细胞测定模式中所进行的空白检测(比如开电源后和自动清洗后进行的)的判断标准更严格,设定的值为数分之一以下。 Blank check (for example, after opening the power supply and the automatic cleaning performed) blank detection criterion in the previous processing sequence than in blood cell measurement mode performed by more stringent criteria, the set division number is one or less. 此外,设定由体液测定模式切换为血液测定模式时,因为本底影响(残留物的影响) 一般不波及血液测定结果,故此项前序列处理不进行。 Further, when the body fluid measurement mode is set by the switching to the measurement mode of the blood, because the background influence (influence residues) generally do not affect the measurement result of blood, so this process is not performed before the sequence. 在体液测定模式中反复测定体液标本时,因为通常不会受到本底的影响,所以也不进行前序列处理。 Repeated measurement specimens of body fluids in a body fluid measurement mode, is generally not affected because of the background, the sequence of processes is not performed before. 但是,体液标本也有的含有大量粒子,因此, 当体液标本分析结果超出一定值时,界面上会出现"测定结果太高,有可能影响下次标本测定,将进行空白检测。请按"确认"。"等信息,通知操作人员有可能影响下面的标本分析结果。 However, some specimens of body fluids also contain a large number of particles, and therefore, when the body fluid sample analysis result exceeds a certain value, appears on the screen, "the result of the measurement is too high, it may affect the next sample measurement, the blank check, please press the" confirm " . "and other information to inform the operator that may affect the results of sample analysis below. 操作人员按"确认"按钮, 即可进行空白检测。 The operator press the "OK" button to blank check. 此时,界面上设有"中止"按钮,操作人员只要按"中止"按钮,也可以不进行空白检测,移至待机界面。 At this time, with the interface "suspend" button, the operator just press "suspend" button, the blank may not be detected, switch to a standby interface. 如果未进行空白检测,则最好对测定结果标注可信度低的符号。 If a blank check is not performed, the label preferably has a low reliability symbols on the measurement result. 这样做可以仅限定于必要的时候追加空白检测,以防止时间和试剂类的浪费。 Doing so may be limited to only when necessary additional blank check, to prevent the waste of time and reagents. 图9为测定模式从血液测定模式切换为体液测定模式时实施的前序列处理步骤的流程图。 9 is a flowchart of processing steps before a sequence of measurement embodiment mode is switched from the blood measurement mode to a body fluid measurement mode.标本分析仪1通过在测定装置2测定空白试样来实施空白检测(步骤S31),微机6将测定结果与一定容许值比较, 判断测定结果是否低于容许值(步骤S32)。当测定结果低于容许值时, 微机6结束前序列运行,恢复处理。当测定值大于容许值时,微机6判断是否进行了规定次数(比如三次)空白检测(步骤S33),如果空白检测次数未达到规定次数,则返回步骤S31,在上述规定次数内再次实施空白检测。如果在规定次数内空白检测测定结果仍未低于容许值,则在显示,操作部分7上显示空白测定结果和包括"确认"按钮、"空白检测"按钮、"自动清洗"按钮在内的画面(步骤S34)。如果操作人员按"确认"按钮(步骤S35),则微机6结束前序列运行,恢复处理。如果按"空白检测"按钮(步骤S36),则微机6将处理返回到步骤S31再次进行空白检测。如果按"自动清洗"按钮(步骤S37),则微机6实施用专用清洗液自动清洗后(步骤S38),将处理返回到步骤S31,再次进行空白检测。上述前序列处理结束后,标本分析仪l返回待机状态(步骤Sll)。操作人员开始体液测定时,与血液标本的手动测定时一样,将测定装置2的标本吸移嘴18插入标本容器中的体液标本,按开始开关。微机6 接到开始测定指示后(步骤S12)开始吸移体液标本(步骤S13)。体液标本吸移后,与血液标本一样体液标本被导入采样阀91。由反应仓13制备RBC/PLT测定试样(步骤S15)。然后,由白细胞检测器41测定DIFF测定试样,由RBC/PLT检测器42测定RBC/PLT须^定试样(步骤S17)。在体液测定模式的状态下,在白细胞检测器41测定的仅为DIFF 测定试样,因此,即使测定时间比血液测定模式的测定时间更长,也可能比血液测定时更短时间内完成测定。如此,通过将体液测定的测定时间延长得比血液测定的测定时间长,可以提高粒子浓度低的体液标本的分析精度。测定时间越长,计数的粒子数就会越多,测定精度就会提高。但是,测定时间过长,标本处理能力会下降,同时将测定试样输送到白细胞检测器41的注射泵的能力有限,因此以2〜6倍为妥。在本实施方式中,将体液测定模式时的测定时间设定为血液测定模式时的3倍。另一方面,RBC/PLT测定试样在任何测定模式下都是导入电阻式检测器41,在一定流束条件下进行测定。然后根据测定所得特征信息进行分析处理(步骤S19),分析结果输出到数据处理装置3的显示器302 (步骤S21)。在血液测定模式下的分析处理中,分析DIFF散点图等,算出五种白细胞亚类(中性细胞:NEUT、淋巴细胞:LYMPH、 单核细胞:MONO、嗜酸细胞:EO、嗜碱细胞:BASO)的信息(数量和比率),但在体液测定模式下的分析处理中,因为有时血细胞数很少或受到破损,因此,以部分统合的形式分类为二种亚类(单核细胞:MN、多核细胞:PMN)。淋巴细胞和单核细胞属于单核细胞,嗜中性细胞、嗜酸性细胞和嗜碱性细胞属于多核细胞。这种分类算法与在血液测定模式下的分析处理中所说明的算法相同,故省略说明。接着,对在步骤S19取得的分析结果和容许值(所定阀值)进行比较(步骤S22)。此容许值与步骤S10的前序列处理中的空白检测中使用的容许值为同一值。当分析结果大于容许值时(步骤S22中选"Y"), 则在步骤S23中显示图17所示开始空白检测的确认界面151。此确认界面151上显示有:显示"测定结果太高,有可能影响下面的标本测定。 将进行空白检测。请按"确认"。"信息的信息显示处152、确认按钮153 和取消按钮154。接下来,判断用户输入的是确认按钮153还是取消按钮154 (步骤S24),如果输入的是确认按钮(在步骤S24中选"确认"), 则实施空白检测(步骤S25)。当在步骤S19取得的分析结果小于容许值时(在步骤S22选"N")或输入的是取消按钮时(在步骤S24中选"取消"),则不进行空白检测,返回步骤S5的处理。在体液试样中有时会存在血细胞以外的异常粒子(巨噬细胞和中皮细胞、肿瘤细胞等)。脑脊液中存在这些异常粒子的情况很少见, 但其他体液胸水和腹水中比较常见。因此,不管体液种类如何,要精确地对体液中的血细胞进行分类和计数,就要排除这些异常粒子的影响。因此,本发明基于异常粒子出现在本标本分析仪的DIFF散点图上侧这一新认识,使仪器能够更精确地测定作为目标的体液试样中的白细胞。这一点在前述的传统技术中是没有考虑的。图10为本实施方式标本分析仪1在体液测定模式下测定、分析由体液和白细胞测定用试剂制备的DIFF测定试样所获得的散点图的模式图。散点图的纵轴表示侧向荧光强度(越靠上,荧光强度越强),横轴表示侧向散射光强度(越靠右,散射光强度越强)。散点图的荧光强度弱的区域LF分布有溶血产生的红细胞血影Gc,荧光强度强的区域HF分布有中皮细胞等异常粒子,中间区域MF分布有单核白细胞Mc、 多核白细胞Pc。因此,在散点图的分析中,以分布于除区域LF和HF以外的区域MF的粒子成份为白细胞进行分析,分类为上述二类,并计数。另外,单核白细胞Mc中包含淋巴细胞和单核细胞,多核白细胞Pc中包含中性细胞、嗜酸性细胞和嗜碱性细胞。如此分析体液中的白细胞时,也有时体液中所含血细胞数很少或受损,因此作为临床上有意义的信息,将白细胞分类为单核白细胞和多核白细胞进行计数。另外,体液中有时存在血细胞以外的异常粒子(巨噬细胞和中皮细胞、肿瘤细胞等)。脑脊液中存在这些异常粒子的情况很少见,但其他体液胸水和腹水中比较常见。在图10的散点图中,这种白细胞以外的有核细胞分布于区域HF。在本实施方式中,可以将白细胞以外的有核细胞与白细胞区分,因此,即使体液中含有这种白细胞以外的有核细胞也能够求出正确的白细胞数。通过对出现在区域HF的细胞进行计数,可以提供异常细胞出现的程度。在本实施方式中,根据区分各区域的阀值将各细胞划分为区域LF、 MF和HF,也可以人工改变这一图11是为显示上述散点图分析法的适当性,而比较采用本实施方式的标本分析仪1所得分析结果与采用对照法所得计数结果的附图。被检试样为胸水,图中的"本法"表示本实施方式的标本分析仪1计算出的白细胞数(WBC)和其他异常粒子数(Others) , "Ref'表示对照法(细胞计数池直接计数法(Fuchs-Rosenthal板)和site spin法)计算出的结果。例l、 2、 3都为分析有异常粒子大量出现的胸水的结果, 可以看出,本实施方式的标本分析仪1所得分析结果与对照法之间具有相关关系。图13为作为由血液制备的上述DIFF用测定试样的分析结果显示在数据处理装置3显示器302上的画面100。画面100的上部有显示标本号101的标本号显示区,其旁边设有显示患者属性的属性显示区。 属性显示区具体地说显示标本号、患者ID、患者姓名、出生年月日、 性别、病房、主治医生、测定年月日、测定时间及备注等。属性显示区下部设有显示测定结果的测定结果显示区。测定结果显示区由数页构成,这些页可以通过选 数个标签102来显示画面。标签针对主页面、图表画面和其他项目备有数个。图12为图表标签选择时的显示画面。测定结果显示区的左半部分设有显示测定结果的测定值的测定值显示区103和显示图表的图表显示区104,右半部分设有显示测定结果的分布图的分布图显示区105。测定值显示区显示WBC、 RBC、...、 NEUT#、 ...BASO#、 NEUT%、 ...、 BASO。/。等项目、数据和单位,图表显示区104显示关于WBC、 PLT、 RBC或RET的可作为临床检查上有用信息的标本异常和疾病怀疑的标记结果。分布图显示区105显示六个分布图。左上部的散点图为DIFF用散点图。右上部为WBC/BASO用、左中部为幼稚细胞(IMI)用,右中部为RET用的各散点图。左下部为RBC用直方图,右下部为PLT用直方图。图14为作为由体液制备的上述DIFF用测定试样的测定结果显示在数据处理装置3显示器302上的画面110。画面110的上部有显示标本号的标本号显示区111,其旁边设有患者属性显示区。标本号显示区111的左边显示有表示以体液测定模式进行测定的"F"。据此可以明确了解到,此分析结果是体液测定结果。测定结果显示区由可用标签112 选择的数页构成。在本例中,选择了"体液测定(体液)"的标签。在测定值显示区113上,与血液测定模式的测定结果不同的体液用测定项目名WBC-BF (WBC数)、RBC-BF (RBC数)、MN# (单核细胞数(淋巴细胞+单细胞))、PMN# (多核细胞数(中性细胞+ 嗜碱性细胞+嗜酸细胞))、MN% (白细胞中的单核细胞比率)、PMN% (白细胞中的多核细胞比率)和测定值、单位分别对应显示。在体液测定也与血液测定同样设有图表显示区114。分布图显示区显示有二个分布图115,上部分散点图为DIFF用散点图。下部分为RBC用直方图。图15为在图14画面110中在标签112中选择了"检索BF (Research (BF))标签的例示。此画面除显示检索参数显示区116外,还显示与画面110同样的项目。检索参数显示区116中显示如图10所示存在于区域HF的粒子数"HF-BFW、区域HF内的粒子数与存在于包括区域HF和区域MF在内的区域的粒子数之比"HF-BFy。"、存在于包括区域HF和区域MF在内的区域的粒子数"TC-BF弁"。另,"HF-BF。/。"是HF-BF与TC-BF之比。图16为显示在数据处理装置3显示器302上的存储标本一览显示画面140。 130是患者属性显示区。其上方设有通过标签选择显示测定结果的测定结果显示区。测定结果显示区最左侧列131用于显示测定结果的验证工作未做或已做。 V表示已验证。其右列132用于显示测定模式。 "F"表示体液测定模式的测定结果。如果虽然是在体液测定模式下需要空白检测的高值标本,但未进行空白检测(即在步骤S24选择取消)的话,为了将其表示出来,可以将F倒置标记。 ^上就本发明的标本分析仪的结构和功能,以预先装入标本分析仪为例进行了说明,但也可以通过控制系统来实现该功能,将该控制系统装入传统的血细胞分析仪,让传统的血细胞分析仪发挥本发明的功能。在本实施方式所述结构中,血液测定模式下对白细胞分类与体液测定模式下对白细胞分类各自制备测定试样时的标本量、试剂种类和试剂量都一样。可不限于此,也可以让制备体液测定模式下分类白细胞用测定试样的标本量和试剂量分别多于制备血液测定模式下分类白细胞用测定试样的标本量和试剂量。由于体液测定模式下对白细胞分类测定时间比血液测定模式长,测定所需的测定试样量也多,因此, 这样做可以分别在血液测定模式下的白细胞分类和体液测定模式下的白细胞分类制备适量的测定试样。再者,血液测定模式下的白细胞分类所用试剂种类也可以与体液测定模式下的白细胞分类所用试剂种类不同。在本实施方式中,就用散射光和荧光在体液测定模式下对白细胞分类的结构进行了阐述,但不限于此,也可以用比如散射光和吸收光在体液测定模式下对白细胞分类。吸收光的测定可以将染色白细胞的染色剂与其他试剂一起混入标本,制备测定试样,将该测定试样提供给流动池,使之在流动池内形成试样流,光照该试样流,通过光电二极管等集光元件接收试样流发出的光。白细胞通过流动池中时,光被白细胞吸收,其吸收程度可以作为集光元件的集光量加以捕捉。关于这种吸收光的测定,美国专利第5122453号及美国专利第5138181号公报上有发表。也可测定电阻取代散射光,通过电阻值和吸收光来对白细胞进行分类。

Claims (21)

1.一种标本分析仪,包括: 测定装置,从血液标本或不同于所述血液标本的体液标本制备测定试样,测定所制该测定试样,获取表示所述测定试样中的成份特征的特征信息; 模式设定单元,用于设定测定血液标本的血液测定模式和测定体液标本的体液测定模式其中之一作为运行模式; 第一控制单元,当所述模式设定单元设定了所述血液测定模式时,控制所述测定装置实施血液标本测定运行; 第二控制单元,当所述模式设定单元设定了所述体液测定模式时,控制所述测定装置实施与所述血液标本测定运行不同的体液标本测定运行。 A sample analyzer, comprising: measuring means, the measurement sample from a blood sample or a body fluid differs from the measurement sample preparation, measurement of the blood sample prepared specimen, acquired COMPOSITIONS sample indicates the measurement characteristic information; mode setting means set the measurement mode for measuring the blood sample and measuring the blood measurement mode in which one of a body fluid as a body fluid specimen operating mode; a first control means, when the mode setting unit sets the the blood measurement mode, said control means measuring a blood sample measurement operation embodiment; second control means, when the mode setting unit sets up the body fluid measurement mode, the control apparatus of the embodiment and the measurement of blood different sample measurement operation of measuring a body fluid sample run.
2. 根据权利要求1所述的标本分析仪,其特征在于:所述测定血液标本的运行包括所述测定装置在第一时间测定由所述血液标本制备的测定试样的运行;所述测定体液标本的运行包括所述测定装置在与所述第一时间不同的第二时间测定由所述体液标本制备的测定试样的运行。 The sample analyzer according to claim 1, wherein: said measurement means comprises a running blood samples measuring operation by the measurement of the sample prepared from blood samples in a first time of the measurement; the measurement operating means comprising a body fluid specimen measuring operation by the measurement sample prepared from the body fluid sample at a second time and the first time is different from the measurement.
3. 根据权利要求2所述的标本分析仪,其特征在于:所述第二时间为比所述第一时间长的时间。 The sample analyzer of claim 2, wherein: the second time is longer than the first time period.
4. 根据权利要求1~3之一所述的标本分析仪,其特征在于:所述测定血液标本的运行包括所述测定装置制备含所述血液标本和第一数量试剂的测定试样的运行;所述测定体液标本的运行包括所述测定装置制备含所述体液标本和与所述第一数量不同的第二数量的所述试剂的测定试样的运行。 According to claim any one of the sample analyzer 1 to 3, characterized in that: the operation of blood samples comprising preparing a measurement sample containing said blood sample and the apparatus number of the first reagent of the measurement operation of the measurement ; bodily fluid running the assay sample preparation device comprises a measuring operation of the measurement sample containing specimens of body fluids and different from the first number of the number of second reagent.
5. 根据权利要求1所述的标本分析仪,其特征在于:所述测定血液标本的运行包括所述测定装置制备含所述血液标本和第一试剂的测定试样的运行;所述测定体液标本的运行包括所述测定装置制备含所述体液标本和种类不同于所述第一试剂的第二试剂的测定试样的运行。 The sample analyzer of claim 1, wherein: the measurement operation of blood samples comprises a sample measuring operation of the blood sample and the first reagent was prepared containing the measurement means; said body fluid measurement running the assay sample preparation device comprises a body fluid sample containing said species and a second measurement sample is different from the operation of the first reagent reagent.
6. 根据权利要求l所述的标本分析仪,其特征在于:还包括空白检测控制单元,用于控制所述测定装置在从所述体液标本制备所述测定试样前,测定不含所述血液标本和所述体液标本的空白试样。 6. The sample analyzer according to claim l, wherein: the blank further comprises a detection control unit for controlling the measuring means prior to the measurement sample prepared from the body fluid sample, the measurement does not contain the blood samples and a blank sample of the bodily fluid sample.
7. 根据权利要求1所述的标本分析仪,其特征在于:还具备比较单元和清洗控制单元;比较单元用于将对所述测定装置测定由体液标本制备的测定试样所得特征信息进行分析获得的分析结果与所定标准值进行比较;清洗控制单元用于控制所述测定装置在所述比较单元比较认为该分析结果大于所述标准值时实施清洗运行。 The sample analyzer of claim 1, characterized by: further comprising a comparison unit and a purge control unit; means for comparing the measurement unit will be analyzed by measuring the measurement sample obtained feature information prepared specimens of body fluids the results obtained are compared with a predetermined reference value; purge control means for controlling said measuring means in said comparing unit compares that running washed embodiment the analysis result is greater than the standard value.
8. 根据权利要求7所述的标本分析仪,其特征在于:还具备禁止手段,当所述分析结果大于所述标准值时,禁止让所述清洗控制单元控制所述测定装置实施所述清洗运行。 8. The sample analyzer of claim 7, characterized by: further comprising means for prohibiting, when the analysis result is greater than the standard value, prohibits the purge control unit controls the measurement apparatus according to the washing run.
9. 根据权利要求1所述的标本分析仪,其特征在于:所述体液标本选自包括脑脊液、胸水、腹水、心囊液、关节液、腹膜透析的透析液和腹腔内清洗液在内的液体。 9. The sample analyzer of claim 1, wherein: said sample is selected from bodily fluid comprises cerebrospinal fluid, pleural effusion, ascites, pericardial fluid, synovial fluid, intraperitoneal dialysate and peritoneal dialysis, including the washing liquid liquid.
10. —种标本分析仪,包括:测定装置,从血液标本或不同于所述血液标本的体液标本制备测定试样,测定所制该测定试样,获取表示所述测定试样中的成份特征的特征信息;模式设定单元,用于设定测定血液标本的血液测定模式和测定体液标本的体液测定模式其中之一作为运行模式;第一分析单元,当所述模式设定单元设定了所述血液测定模式时, 根据所述测定装置测定从所述血液标本制备的测定试样所获取的所述特征信息进行第一分析处理;第二分析单元,当所述模式设定单元设定了所述体液测定模式时,根据所述测定装置测定从所述体液标本制备的测定试样所获取的所述特征信息进行不同于所述第一分析处理的第二分析处理。 10. - kind of sample analyzer, comprising: measuring means, said blood specimen from a blood sample or a body fluid sample is different from the prepared measurement sample, the measurement of the measurement sample prepared, expressed COMPOSITIONS obtain the measurement sample characteristic information; mode setting means set the measurement mode for measuring the blood sample and measuring the blood measurement mode in which one of a body fluid as a body fluid sample mode of operation; a first analysis unit, when the mode setting unit sets the the blood measurement mode, the measurement apparatus according to the measured characteristic information of the measurement sample prepared from a blood sample acquired a first analysis process; second analysis unit, when the mode setting unit when the body fluid measurement mode, the second analysis process is different from the first analysis process based on the feature information of said measuring means for measuring the body fluid measurement sample prepared from a specimen obtained.
11. 根据权利要求10所述的标本分析仪,其特征在于: 所述第一分析处理包括将白细胞分类为至少四类的处理; 所述第二分析处理包括将白细胞分类为少于所述第一分类处理的类别的处理。 11. The sample analyzer of claim 10, wherein: the first analysis processing includes processing at least four leukocyte is; and the second analysis process includes less than said first leukocyte processing categories a classification process.
12. 根据权利要求ll所述的标本分析仪,其特征在于:所述第二分析处理将白细胞分类为多核白细胞和单核白细胞。 12. The sample analyzer according to claim ll, wherein: said second analysis process to classify leukocytes monocytes and polymorphonuclear leukocytes.
13. 根据权利要求10~12之一所述的标本分析仪,其特征在于: 所述第二分析处理包括分析由所述体液标本制备的测定试样中所含一定粒子的处理;所述第一分析处理不包括分析由所述血液标本制备的测定试样中所含所述所定粒子的处理。 13. The sample analyzer according to claim one of claim 10 to 12, wherein: said second analysis processing includes processing the measurement sample prepared by the sample fluid contained in a certain particle analysis; the first It does not include an analysis process of the analysis process contained in the measurement sample prepared from the blood samples in a predetermined particles.
14. 根据权利要求13所述的标本分析仪,其特征在于:所述一定粒子包括选自由巨噬细胞、中皮细胞、肿瘤细胞及其组合构成的群体的粒子。 14. The sample analyzer according to claim 13, wherein: said particles comprise particles composed of certain groups selected from the group consisting of macrophages, mesothelial cells in the tumor cells, and combinations thereof.
15. —种标本分析仪,包括:测定装置,从血液标本或不同于所述血液标本的体液标本制备测定试样,测定所制该测定试样,获取表示所述测定试样中的成份特征的特征信息;模式切换单元,用于将运行模式从测定血液标本的血液测定模式切换到测定体液标本的体液测定模式;空白检测控制单元,当所述模式切换单元将运行模式从测定血液标本的血液测定模式切换到测定体液标本的体液测定模式时,控制所述测定装置测定既不含所述血液标本也不含所述体液标本的空白试样。 15. - kind of sample analyzer, comprising: measuring means, said blood specimen from a blood sample or a body fluid sample is different from the prepared measurement sample, the measurement of the measurement sample prepared, expressed COMPOSITIONS obtain the measurement sample characteristic information; mode switching unit for switching the operating mode from the blood measurement of a blood sample of a body fluid measurement mode to a body fluid measurement mode measurement specimen; blank detection control unit, the mode switching unit when the operation mode from the measurement of blood samples blood measurement mode to a body fluid measurement mode, the measurement of specimens of body fluids, controlling the measuring means for measuring the blood sample containing neither white nor the body fluid sample specimen.
16. 根据权利要求15所述的标本分析仪,还包括: 分析单元,通过分析所述测定装置测定由所述体液标本制备的测定试样得出的所述特征信息获取分析结果;判断单元,在所述模式切换单元将运行模式从血液测定模式切换到体液测定模式时测定所述空白试样的基础上,根据所述分析结果判断是否需要测定装置再次测定所述空白试样。 16. The sample analyzer of claim 15, further comprising: an analyzing unit acquires the analysis result by analyzing the characteristic information obtained by the measuring means of the body fluid measurement sample prepared by the measurement sample; determining means, determined on the basis of the blank sample when the mode switching unit switches the mode from the blood measurement mode to the body fluid measurement mode, based on the analysis results of the measurement apparatus determines whether the blank sample was measured again.
17. 根据权利要求16所述的标本分析仪,其特征在于:还包括指示接受单元,当所述判断单元判断测定装置有必要再次测定所述空白试样时,接受开始所述空白试样测定的指示或取消所述空白试样测定的指示。 17. The sample analyzer according to claim 16, characterized in further comprising: instruction accepting unit, when the determining unit determines that it is necessary to re-measurement device of the sample blank measurement, the measurement starts receiving the sample blank determining the cancellation indication or an indication of sample blank.
18. 根据权利要求17所述的标本分析仪,还包括: 显示器,显示所述分析单元取得的分析结果;显示控制单元,控制所述显示器在所述指示接受单元接到取消所述空白试样测定的指示后,显示分析单元所得分析结果,以便能够确认所述空白试样测定已取消。 18. The sample analyzer according to claim 17, further comprising: a display to display the analysis result obtained by said analysis means; display control unit controls the display unit to accept the instruction to cancel the blank sample after the measurement indication, it displays the obtained analysis result of the analysis unit, so that the measurement can be confirmed that the sample blank has been canceled.
19. 根据权利要求15〜18之一所述的标本分析仪,其特征在于-还具有第二空白检测控制单元,用于当运行模式设定为所述血液测定模式或运行模式既没设定为所述血液测定模式,也没设定为所述体液测定模式时,控制所述测定装置测定所述空白试样。 19. The sample analyzer according to claim one of 15~18, characterized in that - the blank further comprising a second detection control means for, when the operation mode is set to the blood measurement mode or operation mode set neither when the blood measurement mode, nor the body fluid measurement mode is set, the control means determining the measurement of the sample blank.
20. 根据权利要求19所述的标本分析仪,其特征在于: 还包括第一判断单元和第二判断单元,第一判断单元用于比较根据所述空白检测控制单元的控制所测定的空白试样的第一测定值和第一阀值,判断所述第一测定值是否在容许范围内;第二判断单元用于比较根据所述第二空白检测控制单元的控制所测定的空白试样的第二测定值和第二阀值,判断所述第二测定值是否在容许范围内;所述第一阀值比所述第二阀值小。 20. The sample analyzer according to claim 19, characterized in further comprising: a first judgment unit and a second determination unit, a first determination unit for comparing the measured blank test based on the control of the control unit detecting the blank like the first measurement value and a first threshold value, determining whether the first measurement value is within an allowable range; a second judgment unit for comparing the blank sample measured based on the control of the second control unit detecting a blank a second measurement value and the second threshold value, determining whether the second measured value is within an allowable range; the first threshold value is smaller than the second threshold value.
21. —种标本分析仪的分析控制系统,包括: 一个测定试样制备控制系统,用于控制完成从血液标本或不同于该血液标本的体液标本制备测定试样的步骤;一个测定试样测定控制系统,用于控制完成测定所制测定试样的一个特征信息获取控制系统,用于控制完成获取表示所述测定试样所含成份特征的特征信息的步骤;一个运行模式设定控制系统,用于控制完成设定测定血液标本的血液测定模式和测定体液标本的体液测定模式其中之一作为运行模式的步骤;及一个测定试样选择测定控制系统,用于完成当设定为所述血液测定模式时,实施血液标本测定运行,测定从所述血液标本制备的测定试样,当设定为所述体液测定模式时,实施与所述血液标本测定运行不同的体液标本测定运行,测定由所述体液标本制备的测定试样的步骤。 21. - kind of sample analyzer analysis control system, comprising: a measurement specimen preparation control system, for controlling the completion of step a body fluid sample measurement sample prepared from blood samples or different from the blood samples; a measurement sample a control system for controlling the completion of a measurement sample prepared wherein the measurement information acquisition control system for controlling the complete feature information acquiring step represents the features of the components contained in the sample was measured; a mode setting operation control system, complete blood measurement mode for controlling the set of blood samples and measuring a body fluid measurement mode in which one of a body fluid specimen as the operating mode measuring step; a sample selection and measurement control system determines, for completing a setting of the blood when the measurement mode, the blood sample measurement operation embodiment, the measurement sample prepared from the blood sample when the body fluid measurement mode is set, the different embodiments of the sample measurement operation of the blood fluid sample measurement operation, as determined by said step measurement sample prepared specimens of body fluids.
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CN106769805A (en) * 2017-01-22 2017-05-31 江西特康科技有限公司 Cell counting and sorting device

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