CN106769805A - Cell count sorter - Google Patents
Cell count sorter Download PDFInfo
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- CN106769805A CN106769805A CN201710046115.8A CN201710046115A CN106769805A CN 106769805 A CN106769805 A CN 106769805A CN 201710046115 A CN201710046115 A CN 201710046115A CN 106769805 A CN106769805 A CN 106769805A
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- cell
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- cell count
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- sheath fluid
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- 239000012530 fluid Substances 0.000 claims abstract description 68
- 238000002356 laser light scattering Methods 0.000 claims abstract description 33
- 238000002347 injection Methods 0.000 claims abstract description 16
- 239000007924 injection Substances 0.000 claims abstract description 16
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 11
- 230000004308 accommodation Effects 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 6
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 5
- 229910052709 silver Inorganic materials 0.000 claims description 5
- 239000004332 silver Substances 0.000 claims description 5
- 238000003780 insertion Methods 0.000 claims description 3
- 230000037431 insertion Effects 0.000 claims description 3
- 239000004065 semiconductor Substances 0.000 claims description 3
- 238000007747 plating Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 11
- 210000004027 cell Anatomy 0.000 description 79
- 239000007788 liquid Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 241000463219 Epitheca Species 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 229910052571 earthenware Inorganic materials 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 210000000265 leukocyte Anatomy 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 238000010224 classification analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000008151 electrolyte solution Substances 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000004493 neutrocyte Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000000790 scattering method Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4915—Blood using flow cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Optical Measuring Cells (AREA)
Abstract
The present invention provides a kind of cell count sorter, including a counter housing, counter housing connects a generating laser, the top of counter housing is sequentially provided with jet base from top to bottom, gem hole bracket and counting body, the centre of jet base is provided with injection fluid apertures, injection fluid apertures is used to inject cell to be measured, interior sheath fluid entrance is provided with beside injection fluid apertures, the end of gem hole bracket is provided with outer sheath fluid entrance, the center of gem hole bracket bottom is provided with the gem hole passed through for cell and interior sheath fluid, counting body is used to count the cell for flowing through gem hole, laser light scattering lens are provided with below gem hole, laser light scattering lens are provided with a through hole, outer sheath fluid entrance inserts through hole, scattered signal after the laser sent by collecting generating laser is scattered by the cell in laser light scattering lens is classified with to cell.The present invention can be counted and laser light scattering classification to cell simultaneously, reduce equipment cost, lift testing efficiency.
Description
Technical field
The present invention relates to technical field of medical instruments, more particularly to a kind of cell count sorter.
Background technology
Blood routine examination is clinically one of most common laboratory examination.Blood routine examination project mainly include red blood cell,
Leucocyte, blood platelet, hemoglobin and leukocyte differential count etc., it is most basic physical examination project in clinical examination, significance of which
Be the early indication it can be found that many systemic diseases, diagnosis whether anaemia, if having a disease in the blood system, react marrow
Hematopoiesis function etc..
Wherein leucocyte (white blood cell, abbreviation WBC), is the class cell in blood.Leucocyte is also usual
It is referred to as immunocyte.Under the microscope it can be seen that, volume ratio is larger in haemocyte, quantity is fewer.With nucleus.Its
Main Function is phagocytosis bacterium, defence disease.Leucocyte is detected by using five sorting techniques at present, i.e., leucocyte is divided into pouring
Bar cell, monocyte, acidophilia, basicyte, neutrophil leucocyte, the inspection of leucocyte is realized especially by five classifying apparatus
Survey, five classification can make detection more accurate.
With the development with rapid changepl. never-ending changes and improvements of computer technology in recent years, the sorting technique of blood cell analysis five employs laser and dissipates
Penetrate to detect cell interior structure, by laser irradiating cell, different classes of cell interior grain structure is different, dissipates laser
Penetrate signal also just different.Cell count is then mainly carried out by electrical impedance method.But current most five classifying apparatus are by cell count
Two components are designed to laser light scattering, are detected respectively, cause high cost, apparatus structure dispersion influences testing efficiency.This
Outward, when cell passes through flow cell, laser is irradiated on cell traditional laser scattering method, and reception cell is carried out in low-angle
Scattered signal, because laser light scattering signal is weak, is easily disturbed, it is difficult to accurate laser light scattering signal is obtained, only by light
Scattered signal is strengthened in electric multiplier tube and fluorescent staining.
The content of the invention
It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention
Be to propose a kind of cell count sorter, cell can be counted simultaneously and laser light scattering classification, reduce equipment into
This, lifts testing efficiency.
A kind of cell count sorter, including a counter housing, the counter housing connect a generating laser,
The top of the counter housing is sequentially provided with jet base, gem hole bracket and counts body, the jet pipe from top to bottom
The centre of seat is provided with injection fluid apertures, and the injection fluid apertures is used to inject cell to be measured, and interior sheath is provided with beside the injection fluid apertures
Liquid entrance, the end of the gem hole bracket is provided with outer sheath fluid entrance, and the center of the gem hole bracket bottom is provided with for thin
The gem hole that born of the same parents and Nei sheath fluids pass through, the counting body is used to count the cell for flowing through the gem hole, the treasured
Laser light scattering lens are provided with below stone hole, the laser light scattering lens are provided with a through hole, the outer sheath fluid entrance insertion institute
Through hole is stated, the scattering after being scattered by the cell in the laser light scattering lens by collecting the laser that the generating laser sends
Signal is classified with to cell.
According to the cell count sorter that the present invention is provided, cell to be measured, interior sheath fluid are injected by injecting fluid apertures first
Cell is wrapped up from after the entrance of interior sheath aperture entrance, the cell for having wrapped up interior sheath fluid is passed sequentially through from gem hole, now by test bag
The resistance antinoise signal for having wrapped up in the cell of interior sheath fluid realizes counting to cell, and the cell for having counted afterwards enters laser light scattering lens
It is interior, while outer sheath fluid enters laser light scattering lens from outer sheath aperture entrance, now by collecting the laser quilt that generating laser sends
The scattered signal after cell scattering in laser light scattering lens, because the scattered signal of different types of cell is different, so that real
Now to the classification of cell, finally realize and cell counted on a device and laser light scattering classification, testing efficiency is high,
The equipment cost in separately test is reduced simultaneously.
In addition, the cell count sorter provided according to the present invention, can also have following additional technical characteristic：
Further, the laser light scattering lens include the first ellipsoid, and are oppositely arranged with first ellipsoid
Second ellipsoid, is provided with lens face between first ellipsoid and second ellipsoid, the generating laser is just to institute
Lens face setting is stated, the outer surface of first ellipsoid is provided with silver coating.
Further, the cell count sorter also includes scattered signal collector and Fresnel Lenses, described to dissipate
Penetrate signal collector just to set the concave surface of first ellipsoid, the Fresnel Lenses is located at second ellipsoid and institute
State between scattered signal collector.
Further, the lens face at least includes vertical component effect, the vertical component effect and swashing that the generating laser sends
Light is vertical.
Further, the gem hole bracket includes ring part and the tapered portion being connected with the ring part, the interior sheath
Liquid entrance inserts the inside of the tapered portion, and the outer sheath fluid entrance is located at the outside of the tapered portion.
Further, the counter housing includes pedestal, and the inside of the pedestal is provided with accommodation space, and the laser dissipates
Lens are penetrated to be located in the accommodation space.
Further, the cell count sorter also includes waste collection pipe, and the waste collection pipe is located at described
The bottom of pedestal, the waste collection pipe is just set to the through hole.
Further, the generating laser uses semiconductor laser diode as lasing light emitter.
Further, a diameter of 70um~80um of the gem hole, including endpoint value.
Further, a diameter of 75um of the gem hole.
Brief description of the drawings
The structural representation of the cell count sorter that Fig. 1 is provided for one embodiment of the invention；
Fig. 2 is the internal structure schematic diagram of the cell count sorter of Fig. 1；
Fig. 3 is the structural representation of gem hole bracket in Fig. 1；
Fig. 4 is the structural representation of laser light scattering lens in Fig. 1.
Main element symbol description：
Cell count sorter 10；Counter housing 11；
Generating laser 12；Jet base 13；
Gem hole bracket 14；Count body 15；
Injection fluid apertures 131；Interior sheath fluid entrance 132；
Outer sheath fluid entrance 141；Gem hole 142；
Ring part 143；Tapered portion 144；
Laser light scattering lens 16；Through hole 161；
Pedestal 111；Accommodation space 1111；
First ellipsoid 162；Second ellipsoid 163；
Lens face 164；Vertical component effect 1641；
Scattered signal collector 17；Fresnel Lenses 18；
Waste collection pipe 19；
Following specific embodiment will further illustrate the present invention with reference to above-mentioned accompanying drawing.
Specific embodiment
To enable objects, features and advantages of the present invention more obvious understandable, below in conjunction with the accompanying drawings to tool of the invention
Body implementation method is described in detail.Some embodiments of the invention are given in accompanying drawing.But, the present invention can be with many not
With form realize, however it is not limited to embodiment described herein.On the contrary, the purpose for providing these embodiments is made to this
Disclosure of the invention content is more thorough comprehensive.
It should be noted that when element is referred to as " being fixedly arranged on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connected to
To another element or may be simultaneously present centering elements.Term as used herein " vertical ", " level ", " left side ",
" right side ", " on ", D score and similar statement for illustrative purposes only, rather than indicating or imply signified device or unit
Part with specific orientation, with specific azimuth configuration and operation, therefore must be not considered as limiting the invention.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be being fixedly connected, or being detachably connected, or be integrally connected；It can be machine
Tool is connected, or electrically connected；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, can be two units
Connection inside part.For the ordinary skill in the art, can as the case may be understand above-mentioned term in this hair
Concrete meaning in bright.Term as used herein " and/or " include one or more related Listed Items arbitrary and
All of combination.
Fig. 1, the cell count sorter 10 that one embodiment of the invention is provided, including a counter housing 11 are refer to,
The counter housing 11 connects a generating laser 12, and the top of the counter housing 11 is sequentially provided with jet from top to bottom
Base 13, gem hole bracket 14 and counting body 15.
Fig. 2 and Fig. 1 is refer to, the centre of the jet base 13 is provided with injection fluid apertures 131, and the injection fluid apertures 131 is used
In cell to be measured is injected, during specific implementation, an earthenware can be set by injection fluid apertures 131, by the earthenware
Injection cell to be tested.
The side of the injection fluid apertures 131 is provided with interior sheath fluid entrance 132, and the interior sheath fluid entrance 132 is used to inject interior sheath
Liquid, interior sheath fluid is specifically as follows streaming sheath fluid, and because cell is non-conductive in itself, and streaming sheath fluid is a kind of electrolyte solution, can
With conduction, during specific implementation, it is possible to use syringe, then connect flexible pipe and interior sheath fluid is injected by interior sheath fluid entrance 132, together
When the flow velocity of interior sheath fluid can be controlled by controlling syringe, it is generally the case that the flow velocity of sheath fluid is more than by note in control
The flow velocity of the cell to be measured of liquid shoot hole 131.
The end of the gem hole bracket 14 is provided with outer sheath fluid entrance 141, and the outer sheath fluid entrance 141 is used to inject epitheca
Liquid, outer sheath fluid is specifically as follows enzyme cleaning fluid, for cleaning cell, during specific implementation, it is also possible to uses syringe, then connects
The just outer sheath fluid of flexible pipe injects outer sheath fluid by outer sheath fluid entrance 141, while epitheca can also be controlled by controlling syringe
The flow velocity of liquid, it is generally the case that the flow velocity of the flow velocity more than interior sheath fluid of the outer sheath fluid of control.
Fig. 3 and Fig. 2 is refer to, the center of the bottom of gem hole bracket 14 is provided with the treasured passed through for cell and interior sheath fluid
Stone hole 142, during specific implementation, the flexible pipe in earthenware and interior sheath fluid entrance 132 in the injection fluid apertures 131 is just to the treasured
Stone hole 142 is set, to ensure that cell to be measured and interior sheath fluid can enter gem hole 142.A diameter of 70um of gem hole 142~
80um, including endpoint value, specifically in the present embodiment, a diameter of 75um of the gem hole 142, the general cell of the numeric ratio
Size it is somewhat larger, therefore can be just met for having wrapped up the cell of interior sheath fluid one by one by gem hole 142, and will not
Cell overlap is caused to pass through the situation of gem hole 142.In the present embodiment, the gem hole bracket 14 include ring part 143 and with
The tapered portion 144 of the connection of the ring part 143, the interior sheath fluid entrance 132 inserts the inside of the tapered portion 144, described outer
Sheath fluid entrance 141 is located at the outside of the tapered portion 144.
The body 15 that counts is used to count the cell for flowing through the gem hole 142, during specific implementation, the meter
Number body 15 is counted using electrical impedance method to cell, and required parts are the structure of prior art, do not do superfluous herein
State.Cell to be measured can simultaneously wrap up interior sheath fluid when entering gem hole 142, ensure that cell has the resistance antinoise signal of stabilization.
During counting, the interior sheath fluid due to cell outer wrapping, therefore when cell passes through gem hole 142, resistance antinoise signal, 1 can be produced
Signal represents 1 cell, and signal is bigger, represents that cell volume is bigger, so as to realize the analysis to cell volume.
Fig. 2, Fig. 3 and Fig. 4 are refer to, the lower section of the gem hole 142 is provided with laser light scattering lens 16, the laser light scattering
Lens 16 are provided with a through hole 161, and the outer sheath fluid entrance 141 inserts the through hole 161, are swashed with ensureing that epitheca liquid energy enough enters
In light dispersing lenses 16, by collecting laser that the generating laser 12 sends by the cell in the laser light scattering lens 16
Scattered signal after scattering is classified with to cell.Specifically, the jet base 13, gem hole bracket 14, counting body
15 and the through hole 161 be coaxially disposed.Additionally, in the present embodiment, the counter housing 11 includes pedestal 111, the pedestal
111 are specially square structure, and the inside of the pedestal 111 is provided with accommodation space 1111, specifically can by way of hollow out reality
Existing, the laser light scattering lens 16 are in the accommodation space 1111.
According to the cell count sorter that the present embodiment is provided, cell to be measured is injected by injecting fluid apertures 131 first, it is interior
Sheath fluid wraps up cell from after the entrance of interior sheath aperture entrance 132, and the cell for having wrapped up interior sheath fluid is passed sequentially through from gem hole 142, now
Counting to cell is realized by the resistance antinoise signal for testing the cell for having wrapped up interior sheath fluid, the cell for having counted afterwards enters sharp
In light dispersing lenses 16, while outer sheath fluid enters laser light scattering lens 16 from outer sheath aperture entrance 141, now sent out by collecting laser
The laser that emitter 12 sends by laser light scattering lens 16 cell scattering after scattered signal, due to different types of cell
Scattered signal is different, so as to realize the classification to cell, finally realizes and cell is counted and laser on a device
Scattering classification, testing efficiency is high, while reducing the equipment cost in separately test.
Additionally, specific, the generating laser 12 uses semiconductor laser diode as lasing light emitter in the present embodiment,
Wavelength is about 635nm, is obtained in that the light source of stabilization.
Please continue to refer to Fig. 4, the laser light scattering lens 16 include the first ellipsoid 162 and with first ellipsoid
162 the second ellipsoids 163 being oppositely arranged, lens face is provided between first ellipsoid 162 and second ellipsoid 163
164, the generating laser 12 is just set to the lens face 164, and the outer surface of first ellipsoid 162 is provided with silver-plated
Layer.The lens face 164 at least includes vertical component effect 1641, the laser that the vertical component effect 1641 sends with the generating laser 12
Vertically.
Please continue to refer to Fig. 2, the cell count sorter 10 also includes that scattered signal collector 17 and Fresnel are saturating
Mirror 18, the scattered signal collector 17 is just set to the concave surface of first ellipsoid 162, and the Fresnel Lenses 18 is located at
Between second ellipsoid 163 and the scattered signal collector 17.
Difference carries out receiving cell scattered signal, it is necessary to set multiple laser signal receivers with traditional in low-angle,
In the present embodiment, because the outer surface of the first ellipsoid 162 is provided with silver coating, the laser that generating laser 12 sends is dissipated by laser
After the cell scattering penetrated in lens 16, can all be reflected by the silver coating, the laser for then scattering is by the second ellipsoid 163
Afterwards, finally collected by scattered signal collector 17, for follow-up analysis, therefore, it is possible to realize the extensive angle of laser light scattering signal
Receive, and without setting multiple laser signal receivers again, it is only necessary to a laser signal receivers, silver coating can be to swashing
Optical signal is concentrated, and makes the laser light scattering signal of acquisition stronger, is strengthened without reusing photomultiplier or fluorescent staining
Scattered signal, saves cost, and detection can be made more accurate, while avoiding pollution of the fluorescent dye to environment.Additionally, logical
Setting Fresnel Lenses 18 is crossed, laser can be assembled, further enhance the accuracy of detection.It should be understood that in reality
When border is implemented, the analysis of cell volume and the laser light scattering signal of cell when body 15 is counted to cell can also will be counted
Combine carries out classification analysis to cell, it is ensured that the accuracy of testing result.
Fig. 1 and Fig. 2 is refer to, in the present embodiment, the cell count sorter 10 also includes waste collection pipe 19, institute
Waste collection pipe 19 is stated located at the bottom of the pedestal 111, the waste collection pipe 19 is just set to the through hole 161, described
Waste collection pipe 19 is used to collect the cell that test is completed.
Embodiment described above only expresses several embodiments of the invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Shield scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of cell count sorter, an including counter housing, the counter housing connects a generating laser, its
It is characterised by, the top of the counter housing is sequentially provided with jet base, gem hole bracket and counts body, institute from top to bottom
The centre for stating jet base is provided with injection fluid apertures, and the injection fluid apertures is used to inject cell to be measured, the side of the injection fluid apertures
Interior sheath fluid entrance is provided with, the end of the gem hole bracket is provided with outer sheath fluid entrance, and the center of the gem hole bracket bottom sets
There is the gem hole passed through for cell and interior sheath fluid, the counting body to the cell for flowing through the gem hole based on carrying out
Number, is provided with laser light scattering lens below the gem hole, the laser light scattering lens are provided with a through hole, and the outer sheath fluid enters
The mouth insertion through hole, the laser sent by collecting the generating laser is scattered by the cell in the laser light scattering lens
Scattered signal afterwards is classified with to cell.
2. cell count sorter according to claim 1, it is characterised in that the laser light scattering lens include first
Ellipsoid, and the second ellipsoid being oppositely arranged with first ellipsoid, first ellipsoid and second ellipsoid
Between be provided with lens face, the generating laser is just set to the lens face, and the outer surface of first ellipsoid is provided with plating
Silver layer.
3. cell count sorter according to claim 2, it is characterised in that the cell count sorter is also wrapped
Scattered signal collector and Fresnel Lenses are included, the scattered signal collector is just set to the concave surface of first ellipsoid,
The Fresnel Lenses is located between second ellipsoid and the scattered signal collector.
4. cell count sorter according to claim 2, it is characterised in that the lens face at least includes vertical
Portion, the laser vertical that the vertical component effect sends with the generating laser.
5. cell count sorter according to claim 1, it is characterised in that the gem hole bracket includes ring part
And the tapered portion being connected with the ring part, the inside of the interior sheath fluid entrance insertion tapered portion, the outer sheath fluid entrance
Located at the outside of the tapered portion.
6. cell count sorter according to claim 1, it is characterised in that the counter housing includes pedestal,
The inside of the pedestal is provided with accommodation space, and the laser light scattering lens are in the accommodation space.
7. cell count sorter according to claim 6, it is characterised in that the cell count sorter is also wrapped
Waste collection pipe is included, located at the bottom of the pedestal, the waste collection pipe is just set the waste collection pipe to the through hole.
8. cell count sorter according to claim 1, it is characterised in that the generating laser uses semiconductor
Laser diode is used as lasing light emitter.
9. cell count sorter according to claim 1, it is characterised in that a diameter of 70um of the gem hole~
80um, including endpoint value.
10. cell count sorter according to claim 9, it is characterised in that a diameter of 75um of the gem hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046115.8A CN106769805B (en) | 2017-01-22 | 2017-01-22 | Cell count sorter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710046115.8A CN106769805B (en) | 2017-01-22 | 2017-01-22 | Cell count sorter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106769805A true CN106769805A (en) | 2017-05-31 |
| CN106769805B CN106769805B (en) | 2019-06-18 |
Family
ID=58943602
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710046115.8A Active CN106769805B (en) | 2017-01-22 | 2017-01-22 | Cell count sorter |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107449716A (en) * | 2017-07-25 | 2017-12-08 | 深圳市孔雀生物科技有限公司 | A kind of sorting unit of flow cytometer |
| CN109490175A (en) * | 2018-10-17 | 2019-03-19 | 南宁优康电子科技有限公司 | Cell count sorter |
| CN110832302A (en) * | 2017-06-28 | 2020-02-21 | 迪亚戈达夫公司 | Measuring cuvette for counting and/or characterizing cells |
| CN111458280A (en) * | 2020-05-27 | 2020-07-28 | 广州牛顿光学研究院有限公司 | Novel automatic cell counter |
| CN114062228A (en) * | 2020-08-07 | 2022-02-18 | 北京鑫骥金诺医疗器械有限公司 | Bacteria counting device with sheath flow impedance sensor and method thereof |
| CN115598024A (en) * | 2022-11-16 | 2023-01-13 | 长春光吉科技有限责任公司（Cn） | Cell grading counting device and method thereof |
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| JPS5824838A (en) * | 1981-08-05 | 1983-02-14 | Toa Medical Electronics Co Ltd | Blood analyzer |
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