CN107449716A - A kind of sorting unit of flow cytometer - Google Patents
A kind of sorting unit of flow cytometer Download PDFInfo
- Publication number
- CN107449716A CN107449716A CN201710616179.7A CN201710616179A CN107449716A CN 107449716 A CN107449716 A CN 107449716A CN 201710616179 A CN201710616179 A CN 201710616179A CN 107449716 A CN107449716 A CN 107449716A
- Authority
- CN
- China
- Prior art keywords
- nozzle
- quartz curette
- flow cytometer
- ingate
- sorting unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010453 quartz Substances 0.000 claims abstract description 74
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 74
- 238000007789 sealing Methods 0.000 claims description 11
- 239000011148 porous material Substances 0.000 claims description 2
- 239000012530 fluid Substances 0.000 abstract description 28
- 230000000694 effects Effects 0.000 abstract description 6
- 230000005284 excitation Effects 0.000 abstract description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000004575 stone Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
Abstract
The invention discloses a kind of sorting unit of flow cytometer, including：Quartz curette, is arranged on the lower section in the sample feeding hole of flow cytometer, and is arranged on the side of the light-emitting window of the excitation source of flow cytometer, and wherein quartz curette is provided with the perforate cavity below sample feeding hole；Nozzle, the lower section of quartz curette is arranged on, wherein nozzle is provided with the ingate below the perforate cavity in quartz curette and the outlet opening connected with ingate；Wherein, the internal diameter of ingate is bigger than the internal diameter of outlet opening.By the above-mentioned means, sorting unit provided by the present invention, which can reduce, wraps the change in flow that celliferous sheath fluid flows through nozzle, the damage to cell is reduced, to improve the activity of cell after airflow classification.
Description
Technical field
The present invention relates to low cytometric analysis field, more particularly to a kind of sorting unit of flow cytometer.
Background technology
Flow cytometer is the instrument for identifying different types of cell and similar finely ground particle substance.Conventional streaming is thin
Born of the same parents' instrument exports containing transparent flow chamber (Flow cell), usually quartz curette, square structure, both ends.Not celliferous sheath fluid and
Cell suspension injects quartz curette from quartz curette entrance under stress, and not celliferous sheath fluid is wrapped in cell suspension, passes through fluid
Principle of dynamics makes on the longitudinal axis that cell suspension constrains in quartz curette into one thread, and quartz is flowed through together with cell suspension
Cup, flowed out from outlet.Under the constraint of sheath fluid, cell arranges cell suspension one by one, when flowing through the cell detection area of quartz curette, often
The exciting light (such as laser) that individual cell is focused excites and sends optical signal.Cell is excited the light letter sent after light irradiation
Number feature can reflect the form of cell, density, size, biological property etc..
Flow cytometer with sorting function can also will be specifically thin according to the cell of detection or the characteristic of particle
Born of the same parents or grain sorting come out it is further studied, including culture, morphological analysis, sequencing or functional experiment etc..
Specifically, as shown in figure 1, the quartzy cup type flow cytometer with sorting function, conventional flotation-dividing part include
Quartz curette 14 and nozzle 16, nozzle 16 have certain thickness, and containing ingate and outlet opening, both form vertical channel 19 at connection, spray
The bottom of mouth 16 and quartz curette 14 is combined closely, the ingate of nozzle 16 and the outlet of the bottom of quartz curette 14.Piezoelectricity
Device shakes quartz curette, wraps celliferous sheath fluid and is flowed out from nozzle 16, one is broken into the distance below of nozzle 16
The uniform drop of consecutive.Ideally, each drop comprises only a cell, the detection zone that this cell passes through quartz curette 14
When be detected identification.If aim cell is sorted out by way of electrostatic, the drop comprising aim cell is necessary
It is electrically charged.The charging original paper that can change rapidly comprising current potential in quartz curette 14, aim cell reach drop breakpoint by nozzle 16
When, the drop comprising aim cell is electrically charged.There are two or more to keep constant potential difference in the distance below of nozzle 16
Deflecting plates.Deflecting plates, which forms static electric field, can deflect the charged droplets containing aim cell, fall into the collection device of lower section, from
And aim cell is sorted out.
However, the activated state of cell is related to effect or the success or failure of subsequent experimental after sorting.Wherein influence sorting activity
One of influence factor for sorting when damage of the dramatic change of cell flow rate to cell.Assuming that in the cavity of quartz curette 14 cell stream
Speed is V1, and the cell flow rate before the ingate of nozzle 16 to outlet opening is V2, and the flow velocity of nozzle exit orifice cell is V3, existing
In sorting unit, because the ingate of nozzle 16 connects to form vertical channel 19, V2=V3, cell flow rate with both of outlet opening
Changes delta V=V2-V1, for existing sorting unit, V2 and V1 are fixed value so that the sheath fluid comprising cell 13 flows through
The moment of nozzle 16, change in flow is relatively large, easily causes bigger damage to the cell 13 flowed through, has a strong impact on sorting
The activated state of cell afterwards.
The content of the invention
The present invention solves the technical problem of a kind of sorting unit of flow cytometer is provided, can reduce comprising thin
The sheath fluid of born of the same parents flows through the change in flow of nozzle, reduces the damage to cell.
In order to solve the above technical problems, one aspect of the present invention is：A kind of point of flow cytometer is provided
Screening device, including：Quartz curette, is arranged on the lower section in the sample feeding hole of flow cytometer, and is arranged on swashing for flow cytometer
The side of the light-emitting window of light emitting source, wherein quartz curette are provided with the perforate cavity below sample feeding hole；Nozzle, it is arranged on stone
The lower section of English cup, wherein nozzle are provided with the ingate below the perforate cavity in quartz curette and the outlet connected with ingate
Hole；Wherein, the internal diameter of ingate is bigger than the internal diameter of outlet opening.
Wherein, inner wall smooth is made between the ingate of nozzle and outlet opening is in funnelform passage.
Wherein, a diameter of 50 μm, 70 μm, 85 μm, 100 μm, 130 μm or 200 μm of the outlet opening of nozzle.
Wherein, sealing ring is provided between nozzle and quartz curette.
Wherein, the section of the perforate cavity of quartz curette is in rounded shape, and the internal diameter of the ingate of nozzle is less than or equal to stone
The internal diameter in the section of the perforate cavity of English cup.
Wherein, the internal diameter of sealing ring is equal or slightly larger than the internal diameter in the section of the perforate cavity of quartz curette.
Wherein, the rectangular in cross-section or square of the perforate cavity of quartz curette, and the internal diameter of the ingate of nozzle is less than or waited
Length in the section of the perforate cavity of quartz curette.
Wherein, the internal diameter of sealing ring is equal or slightly larger than the length in the section of the perforate cavity of quartz curette.
The beneficial effects of the invention are as follows：It is different from the situation of prior art, presently disclosed flow cytometer
Sorting unit includes：Quartz curette, is arranged on the lower section in the sample feeding hole of flow cytometer, and is arranged on swashing for flow cytometer
The side of the light-emitting window of light emitting source, wherein quartz curette are provided with the perforate cavity below sample feeding hole；Nozzle, it is arranged on stone
The lower section of English cup, wherein nozzle are provided with the ingate below the perforate cavity in quartz curette and the outlet connected with ingate
Hole；Wherein, the internal diameter of ingate is bigger than the internal diameter of outlet opening.By the above-mentioned means, sorting unit provided by the present invention can
Reduce and wrap the change in flow that celliferous sheath fluid flows through nozzle, reduce the damage to cell.
Brief description of the drawings
Fig. 1 is the cutting structural representation of the sorting unit of conventional flow cytometer；
Fig. 2 is the first cutting structural representation of the sorting unit of flow cytometer of the present invention；
Fig. 3 is the second cross-sectional structure schematic diagram of the sorting unit of flow cytometer of the present invention.
Embodiment
The present invention is described in detail with embodiment below in conjunction with the accompanying drawings.
The present invention discloses a kind of flow cytometer, and the flow cytometer includes sorting unit.As Figure 2-3, Fig. 2 is this
First cutting structural representation of the sorting unit of invention flow cytometer；Fig. 3 is the sorting unit of flow cytometer of the present invention
The second cross-sectional structure schematic diagram.The sorting unit includes quartz curette 14 and nozzle 16.
Quartz curette 14 is arranged on the lower section in the sample feeding hole 11 of flow cytometer, and quartz curette 14 is arranged on fluidic cell
The side of the light-emitting window of the excitation source 20 of instrument.Wherein quartz curette 14 is provided with the perforate cavity in the lower section of sample feeding hole 11
141, and wrap celliferous sheath fluid and flow through perforate cavity 141.It should be understood that the light launched from the light-emitting window of excitation source 20 shines
Penetrate in perforate cavity 141, detection identification is carried out with the cell in split vestibule body 141, to carry out sorting cell.
Nozzle 16 is arranged on the lower section of quartz curette 14, and wherein nozzle 16 is provided with the perforate cavity 141 in quartz curette 14
The ingate 18 of side and the outlet opening 191 connected with ingate 18.
In the present embodiment, ingate 18 and the section of outlet opening 191 are in rounded shape, and the internal diameter of ingate 18 is than outlet
The internal diameter in hole 191 is big.
Further, inner wall smooth is made between the ingate 18 of nozzle 16 and outlet opening 191 is in funnelform passage,
Wrap celliferous sheath fluid from the flow nozzle 16 of perforate cavity 141 of quartz curette 14 be in funnelform passage when, the sectional area of passage
Taper into, it is assumed that the flow velocity of cell is V1 in quartz curette cavity, and the cell flow rate before the ingate of nozzle 16 to outlet opening is
V2, the flow velocity of nozzle exit orifice cell are V3, the changes delta V=V2-V1 of cell flow rate, and in the present invention, V1 is fixed value, V2
For the value of the consecutive variations between V1 and V3, thus wrap celliferous sheath fluid to flow acceleration change when flowing through nozzle small, from
And the damage to cell reduces.
In the present embodiment, a diameter of 50 μm (microns) of the outlet opening 191 of nozzle 16,70 μm, 85 μm, 100 μm, 130 μ
M or 200 μm, it should be appreciated that the present invention do not limit a diameter of 50 μm of outlet opening 191 of nozzle 16,70 μm, 85 μm, 100 μm,
130 μm or 200 μm, in other embodiments, the outlet opening 191 of nozzle 16 can also be other sizes, with specific reference to actual feelings
Depending on condition.
It is worth noting that, for the hair of the mankind, general diameter is hereinafter referred to as fine at 60 μm, and 60~90 μm are common
Hair, more than 90 μm are thick hair, and the hair of yellow is typically relatively thicker, is commonly 80~90 μm.And in existing sorting unit,
As shown in figure 1, the ingate of existing nozzle 16 and the diameter of outlet opening are equal, and the ingate and outlet of existing nozzle 16
The diameter in hole is inherently very thin, i.e., the ingate of existing nozzle 16 and the diameter of outlet opening are in micron level, to existing
The transformation of the ingate of nozzle 16 and the diameter of outlet opening needs to pay very big creation labour's.And in order to reduce to flowing through spray
Cell 13 in mouth 16 is caused to damage, and the present invention is further transformed in the nozzle chamber of micron level, and this is to need to pay
Go out great energy and creative thinking；Furthermore the size of the outlet opening 191 of nozzle 16 is gradual towards the direction away from quartz curette 14
Damage can be reduced to the cell for flowing through nozzle 16 by diminishing.
Further, sealing ring 15 is provided between nozzle 16 and quartz curette 14.Both nozzle 16 and quartz curette 14 pass through
Sealing ring 15 closely connects, and reaches sealing effectiveness.
In the present embodiment, the section of the perforate cavity 141 of quartz curette 14 is square, and the ingate 18 of nozzle 16
Internal diameter is less than or equal to the length in the section of the perforate cavity 141 of quartz curette 14.
It should be understood that in other embodiments, the rectangular in cross-section shape of the perforate cavity 141 of quartz curette, and nozzle 16 enters
The internal diameter of oral pore 18 is less than or equal to the internal diameter in the section of the perforate cavity 141 of quartz curette.The internal diameter of wherein sealing ring 15 is equal to
Or slightly larger than quartz curette 14 perforate cavity 141 section interior electrical path length.It should be understood that the perforate cavity 141 of quartz curette is cut
Face can also in rounded shape, ellipticity or other shapes.
Further, the internal diameter of sealing ring 15 is equal or slightly larger than the length in the section of the perforate cavity 141 of quartz curette 14.
It should be understood that in other embodiments, the perforate cavity 141 of quartz curette can be in round table-like setting, and perforate cavity 141 leans on
The internal diameter of the one end in nearly sample feeding hole 11 is bigger close to the internal diameter of the other end of nozzle 16 than perforate cavity 141, can so drop
The sheath fluid comprising cell 13 produces less pressure to nozzle 16 in funnelform passage in quartz curette 14.
The aperture for the ingate 18 that the present embodiment passes through enlarged nozzle 16, the aperture model of the ingate 18 of nozzle 16 can be made
The boundary point of the bottom opening for the perforate cavity 141 for being extended to quartz curette 14 is enclosed, the aperture of ingate 18 of nozzle 16 is equal to stone
The length of side of the bottom opening of the perforate cavity 141 of English cup 14.In the thickness that nozzle 16 allows, ingate 18 and outlet opening 191
Between be made inner wall smooth funnel-shaped structure passage, inject quartz curette 14 from sample feeding hole 11 when wrapping celliferous sheath fluid
Perforate cavity 141 when, line up to excite by the irradiation of excitation source 20 of the detection zone of quartz curette 14 when wrapping celliferous sheath fluid,
Wrap ingate 18 of the celliferous sheath fluid from quartz curette 14 by nozzle 16, flow through in nozzle 16 be in funnelform passage after,
Flowed out from outlet opening 191, due to wrapping celliferous sheath fluid from quartz curette 14 to outlet opening 191, in the section of funnelform passage
Product tapers into, and cell flow rate is gradually increased, cell will not produce dramatic change in flow, greatly reduce the damage to cell
Wound, to improve the activity of cell after airflow classification.
It should be understood that in other embodiments, the sheath fluid of cell 13 is included in quartz curette 14 in funnelform in order to reduce
The pressure of passage, the perforate cavity 141 of quartz curette 14 is preferably provided with least one layer of web plate, the web plate is opened provided with least one
The path that hole, at least a perforate and cell are flowed through to nozzle 16 is corresponding, and cell can be made to be flowed through from the perforate of web plate to nozzle 16
It is interior, and the region that the web plate is not provided with perforate is also equipped with small mesh, can flow through sheath fluid, but resistance can be produced to sheath fluid, from
And the flow velocity that sheath fluid can be reduced by setting web plate to have the function that.Or in other embodiments, the perforate of quartz curette 14
The marginating compartment of cavity 141 is provided with multiple projections, can be by the plurality of raised to sheath fluid generation resistance, to reach reduction sheath fluid
The effect of flow velocity.Or in other embodiments, multiple gears are provided with the marginating compartment of the perforate cavity 141 of quartz curette 14
Plate, resistance can be produced to sheath fluid by multiple baffle plates, to have the function that to reduce the flow velocity of sheath fluid.It is, of course, also possible at other
In embodiment, multiple baffle plates and multiple baffle plates can be provided with the marginating compartment of the perforate cavity 141 of quartz curette 14 simultaneously, it is multiple
Baffle plate can produce resistance, to reach the flow velocity for reducing sheath fluid to sheath fluid jointly towards sample feeding hole 11 by raised and baffle plate
Effect.
To sum up, the sorting unit of presently disclosed flow cytometer includes：Quartz curette, it is arranged on flow cytometer
Sample feeding hole lower section, and be arranged on the side of the light-emitting window of the excitation source of flow cytometer, wherein quartz curette is provided with
Perforate cavity below sample feeding hole；Nozzle, the lower section of quartz curette is arranged on, wherein nozzle is provided with quartz curette
Ingate below perforate cavity and the outlet opening connected with ingate；Wherein, the internal diameter of ingate is bigger than the internal diameter of outlet opening.
By the above-mentioned means, sorting unit provided by the present invention, which can reduce, wraps the change in flow that celliferous sheath fluid flows through nozzle,
Reduce the damage to cell.
Embodiments of the present invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this
The equivalent structure or equivalent flow conversion that description of the invention and accompanying drawing content are made, or directly or indirectly it is used in other correlations
Technical field, it is included within the scope of the present invention.
Claims (8)
- A kind of 1. sorting unit of flow cytometer, it is characterised in that including：Quartz curette, is arranged on the lower section in the sample feeding hole of the flow cytometer, and is arranged on swashing for the flow cytometer The side of the light-emitting window of light emitting source, wherein the quartz curette is provided with the perforate cavity below the sample feeding hole；Nozzle, the lower section of the quartz curette is arranged on, wherein the nozzle is provided with below the perforate cavity in the quartz curette Ingate and the outlet opening that is connected with the ingate；Wherein, the internal diameter of the ingate is bigger than the internal diameter of the outlet opening.
- 2. the sorting unit of flow cytometer according to claim 1, it is characterised in that the ingate of the nozzle and go out Inner wall smooth is made between oral pore is in funnelform passage.
- 3. the sorting unit of flow cytometer according to claim 1, it is characterised in that the outlet opening of the nozzle it is straight Footpath is 50 μm, 70 μm, 85 μm, 100 μm, 130 μm or 200 μm.
- 4. the sorting unit of flow cytometer according to claim 1, it is characterised in that the nozzle and the quartz curette Between be provided with sealing ring.
- 5. the sorting unit of flow cytometer according to claim 4, it is characterised in that the perforate cavity of the quartz curette Section it is in rounded shape, and the internal diameter of the ingate of the nozzle is less than or equal to the section of the perforate cavity of the quartz curette Internal diameter.
- 6. the sorting unit of flow cytometer according to claim 5, it is characterised in that the internal diameter of the sealing ring is equal to Or slightly larger than the quartz curette perforate cavity section internal diameter.
- 7. the sorting unit of flow cytometer according to claim 4, it is characterised in that the perforate cavity of the quartz curette Rectangular in cross-section or square, and the internal diameter of the ingate of the nozzle be less than or equal to the quartz curette perforate cavity cut The length in face.
- 8. the sorting unit of flow cytometer according to claim 7, it is characterised in that the internal diameter of the sealing ring is equal to Or slightly larger than the quartz curette perforate cavity section length.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710616179.7A CN107449716A (en) | 2017-07-25 | 2017-07-25 | A kind of sorting unit of flow cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710616179.7A CN107449716A (en) | 2017-07-25 | 2017-07-25 | A kind of sorting unit of flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107449716A true CN107449716A (en) | 2017-12-08 |
Family
ID=60488259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710616179.7A Pending CN107449716A (en) | 2017-07-25 | 2017-07-25 | A kind of sorting unit of flow cytometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107449716A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521549A (en) * | 2020-05-13 | 2020-08-11 | 洹仪科技(上海)有限公司 | Particle sorting device and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2748919Y (en) * | 2004-09-09 | 2005-12-28 | 刘世明 | High-speed fluid flow sampling device for use in flow mode cell technique |
| CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
| CN105717036A (en) * | 2016-04-29 | 2016-06-29 | 安徽师范大学 | Magnetic stirring type flow cytometry flowing chamber |
| CN106769805A (en) * | 2017-01-22 | 2017-05-31 | 江西特康科技有限公司 | Cell count sorter |
| CN106932341A (en) * | 2016-11-02 | 2017-07-07 | 北京信息科技大学 | A kind of double sheath fluid flow chambers for improving flow cytometer laminar flow stability |
| CN207007669U (en) * | 2017-07-25 | 2018-02-13 | 深圳市孔雀生物科技有限公司 | A kind of sorting unit of flow cytometer |
-
2017
- 2017-07-25 CN CN201710616179.7A patent/CN107449716A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2748919Y (en) * | 2004-09-09 | 2005-12-28 | 刘世明 | High-speed fluid flow sampling device for use in flow mode cell technique |
| CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
| CN105717036A (en) * | 2016-04-29 | 2016-06-29 | 安徽师范大学 | Magnetic stirring type flow cytometry flowing chamber |
| CN106932341A (en) * | 2016-11-02 | 2017-07-07 | 北京信息科技大学 | A kind of double sheath fluid flow chambers for improving flow cytometer laminar flow stability |
| CN106769805A (en) * | 2017-01-22 | 2017-05-31 | 江西特康科技有限公司 | Cell count sorter |
| CN207007669U (en) * | 2017-07-25 | 2018-02-13 | 深圳市孔雀生物科技有限公司 | A kind of sorting unit of flow cytometer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111521549A (en) * | 2020-05-13 | 2020-08-11 | 洹仪科技(上海)有限公司 | Particle sorting device and method |
| CN111521549B (en) * | 2020-05-13 | 2021-01-01 | 洹仪科技(上海)有限公司 | Particle sorting device and method |
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Application publication date: 20171208 |