JPH02287260A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To always keep the state of an automatic analyser constant by determining a washing condition on the basis of the judgement of the state of the automatic analyser in the analysis of a water blank value, a reagent blank value or a control specimen. CONSTITUTION:This automatic analyser can perform automatic analysis and washing operation. At this time, at least one item selected from the change of a water blank value, the stability of a reagent blank value and the analytical result of a control specimen among the measuring results of an absorbancy measuring part 3 is monitored by a measuring result monitor means 4. This means 4 monitors items such as the durnal change and daily variance of the water blank value, the variation within the same measurement and diurnal change of the reagent blank value and the items such as the multi-rule, Qsum or X-Rs of the analytical results in the analysis of the control specimen. Subse quently, a washing condition determination means 5 compares the items monitored by the means 4 with a predetermined reference value to determine the washing condition corresponding to a degree of contamination.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to an automatic analyzer that can automatically perform analysis.

Automatic analyzers are used, for example, in hospitals and the like for clinical tests that automatically and continuously analyze liquid samples such as serum and urine.

(Prior Art) Cleaning is important for automatic analyzers to maintain analysis accuracy. The cuvette is used for cleaning.

These include a pipettor that supplies a sample to a cuvette, a probe that supplies a reagent to a cuvette, etc. To wash a cuvette, detergent is placed in the cuvette, water is supplied from the washing mechanism, and the water is discharged. For detergents, use regular detergents and special detergents with stronger cleaning power. Cleaning conditions such as detergent selection and number of cleaning cycles are determined by the operator based on his or her habits.

(Problem to be solved by the invention) Since the degree of cleaning is determined by the operator based on his/her habits, some equipment may require too much cleaning, wasting cleaning time, or
On the other hand, there is an inconvenience that a shortage affects analysis accuracy.

The present invention provides an automatic analyzer with a cleaning function that can keep the condition of the automatic analyzer constant at all times by monitoring the condition of the automatic analyzer and automatically determining the degree of cleaning to reflect the condition. The purpose is to provide an analytical device.

(Means for Solving the Problems) The present invention deals with the measurement results of the absorbance measuring section 3 in an automatic analyzer as shown in FIG.

A measurement result monitoring means 4 for monitoring at least one of the following: changes in the water blank value, stability of the reagent blank value, and analysis results of the control sample; cleaning conditions for determining cleaning conditions from the results of the measurement result monitoring means 4 and reference values; The condition determination means 5 is also provided.

(effect) Measurement result monitoring means 4 monitors intraoral fluctuations in water blank values.

Daily fluctuations, reagent plan values, fluctuations within the same measurement, daily fluctuations, and when analyzing control samples, the multi-rule, cusum, X-R5, etc. of the analysis results are monitored. The items to be monitored do not need to be all of these, and one or more items may be appropriately selected and monitored.The cleaning condition determining means 5 compares the items monitored by the measurement result monitoring means 4 with predetermined reference values. Establish cleaning conditions according to the degree of contamination. An example of a method for determining cleaning conditions is to set a standard number of cleaning operations using a normal detergent, and then changing the number of times or adding a cleaning operation using a special detergent. However, the method is not limited to this method.

FIG. 2 shows an example of the operation.

Evaluate the water blank value (step SL). The water blank value represents the degree of contamination of the cuvette. If the difference between the daily average water blank value and the initial data is greater than 5 mABS, the cuvette is dirty, so wash all cuvettes once with a special detergent (
Step S2).

Special detergents are highly alkaline and have a higher concentration than regular detergents.

The difference between the average water blank value in the mouth and the initial data is 20 mA.
If it is below BS, it can be determined that the degree of contamination of the cuvette is small. Even in that case, for example when using a sample that is easily contaminated, the maximum intraoral variation of the water blank value is 2 mAB.
If it is larger than S (step S3), wash all cuvettes 10 times with regular detergent (step S3).
4. ).

2 Maximum daily variation of water blank value is 2 m A B
If it is less than or equal to S, evaluate the reagent blank value and proceed to step S.
5) If the intraday variation is greater than 1% of the covariance (CV), wash all cuvettes 10 times with one normal detergent (step S4).

If the diurnal variation of the reagent blank value is less than 1% of the covariance, evaluate the measurement results using the control sample (step S6), and if the results are poor, perform additional washing of all cuvettes 10 times with regular detergent (step S6). S4), and if the results are good, wash all the cuvettes five times with a normal detergent (step S7).

When evaluating reagent blank values, the within-day variation is greater than 1% of the covariance, or the variation within the same measurement is 1% of the covariance.
% (step S8), the reagent probe is washed an additional 30 times with a special detergent (step S9).

The intraday variation of the reagent blank value is less than 1% of the covariance,
In addition, if the variation within the same measurement is 1% or less of the covariance (step S8), if the measurement result with the control sample is poor, the pipetter is washed an additional 30 times with a special detergent (step 511); If the measurement result using the control sample is good, the probe is washed 30 times using a normal detergent (step S9).

After determining the additional cleaning conditions using the special detergent and the normal detergent in this way, the cleaning conditions for the cuvette, pipetter, and probe are determined (step 513), and the cleaning operation is executed.

(Example) FIG. 3 shows an example of an automatic analyzer to which the present invention is applied.

Cuvettes 12 are arranged around the reaction disk 10. A turntable 14 is located near the reaction disk 10.
is provided, and cups containing specimens are arranged on the turntable 14. A running water washing well 17 is provided between the reaction disk 10 and the turntable 14 from which a washing liquid for washing the probe and flow path of the pipetter 16 gushes out. The pipettor 16 is connected to the turntable 4 during analysis operation.
A sample is aspirated from the upper sample cup and injected into the cuvette 12, where it is washed by a well 17 for washing with running water. 18 is a pipettor pump for aspirating the sample into the pipettor 16 and injecting it into the cuvette 12;

This is a diluter pump for pushing out the sample with degassed water.

Dispensers 26a, 26b and reagent storages 28a, 28b are provided for injecting into the cuvette 12 a reagent to be reacted with the sample in the cuvette 12. During the analysis operation, reagents are sucked from the reagent bottles arranged in the reagent storages 28a, 28b by the probes 25a, 25b of the dispensers 26a, 26b and injected into the cuvette 12. 30 is a dispenser pump for aspirating reagents with dispensers 26a and 26b and injecting them into cuvette 12.

27a and 27b are cleaning wells from which a cleaning liquid flows out to clean the probes 25a and 25b of the dispensers 26a and 26b and the channels.

A stirring mechanism 34 is provided near the reaction disk 10 to stir the sample and reagent injected into the cuvette 12.
In addition, in order to optically detect the reaction in the cuvette 12, a spectrometer 3 that can be reciprocated is installed near one reaction disk 10.
6 is provided.

In order to wash the cuvette 12, the reaction disk 1
A cleaning mechanism 38 is provided near 0. 40 is a washing pump for injecting and recovering a washing liquid from the nozzle of the washing mechanism 38 into the cuvette 12. The washing mechanism 38 first sucks the reaction liquid in the cuvette 12 and discharges it to a waste liquid tank (not shown).

For cleaning with a special detergent, the reaction disk 10
A special detergent well 19 is provided between the turntable 14 and the turntable 14 . This special detergent not only cleans the pipetter 16 itself, but also is sucked by the pipetter 16 and supplied to the cuvette 12. Also in the reagent storages 28a and 28b. Special detergent wells 28a and 28b are provided, respectively. This special detergent is the reagent probe 25a.

In addition to cleaning the probe 25b itself, the probe 25a,
25b and supplied to the cuvette 12.

In order to perform cleaning with a regular detergent, the regular detergent is supplied from the pipettor 16 and the reagent probes 25a and 25b through the piping system.

46 is a reservoir for keeping the temperature of constant temperature circulating water constant.

The turntable 14 and the pipettor/diluter pump 18 are controlled by a microcomputer 24 via a sampler control computer 22 and an interface 20. Dispensers 26a, 26b and dispenser pump 30 are controlled by dispenser control computer 32.
and is controlled by a microcomputer 24 via an interface 20. The stirring mechanism 34, the cleaning mechanism 38, and the cleaning pump 40 are connected to the microcomputer 24 via the reaction section control computer 42 and the interface 20.
controlled by The detection output of the spectrometer 36 is log
The data is taken into the microcomputer 24 via the converter and A/D converter 44° and the interface 20.

The absorbance measurement section 3 in FIG. 1 is realized by a spectrometer 36° log conversion section and an A/D conversion section 44, and the measurement result monitoring means 4 and cleaning condition determining means 5 are realized by a microcomputer 24.

The interface 20 further includes a printer 48, a keyboard 50 . A CRT 52 and a floppy disk drive 54 are connected.

(Effects of the Invention) In the present invention, the status of the automatic analyzer is determined from the water blank value, reagent blank value, control sample analysis results, etc., and cleaning conditions for cuvettes, etc. are determined based on those results. It becomes possible to automatically maintain a constant state at all times.

[Brief explanation of drawings]

FIG. 1 is a block diagram showing the main parts of the present invention, FIG. 2 is a flowchart showing an example of the operation, and FIG. 3 is a device configuration diagram showing one embodiment. 3... Absorbance measurement section, measurement result monitoring means, 5.
. . . Washing condition determining means, 16 . . . Pipettor, 19° 29a, 29b . 25b...Reagent probe, 36...Spectrometer.

Claims (1)

[Claims] (1) In an automatic analyzer that can perform analysis automatically and perform cleaning operations, among the measurement results of the absorbance measurement section, changes in water blank value, stability of reagent blank value, and analysis results of control samples An automatic analyzer comprising: a measurement result monitoring means for monitoring at least one item; and a washing condition determining means for determining washing conditions from the results of the measurement result monitoring means and a reference value.