CN204255817U - Blood analyser flow chamber - Google Patents
Blood analyser flow chamber Download PDFInfo
- Publication number
- CN204255817U CN204255817U CN201420751923.6U CN201420751923U CN204255817U CN 204255817 U CN204255817 U CN 204255817U CN 201420751923 U CN201420751923 U CN 201420751923U CN 204255817 U CN204255817 U CN 204255817U
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- CN
- China
- Prior art keywords
- flow chamber
- mounting hole
- micropore
- erte
- electrode slice
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Abstract
The utility model discloses a kind of blood analyser flow chamber, these both sides, blood analyser flow chamber top are provided with HGB power valve and receiving tube mounting hole, an electrode slice mounting hole is arranged at adjacent HGB power valve bottom, below electrode slice mounting hole, there are a sample port and filter washing water inlet in both sides, a quartz window sheet is respectively had before and after below electrode slice mounting hole, in sample port side and with the coaxial cable of quartz window sheet center on Ku Erte jewel micropore is installed, Ku Erte micropore is processed by jewel sheet.The utility model has the advantage of: flow chamber process structure is relatively simple, without sheath stream pipe, without blast tube and cost is low.The utility model does not adopt traditional flow chamber and diluted cup two parts separately to use, but diluted cup is combined use with flow chamber, electrical impedance detection and optical signal detecting are carried out simultaneously in parts, while saving material, also save sample and reagent, and greatly shorten the working time of instrument.
Description
Technical field
The utility model relates to medicine equipment, especially a kind of blood analyser flow chamber.
Background technology
Flow chamber (Flow Chamber) is the core component of differential hematology analyzer.Traditional flow chamber is made up of sample hose, sheath fluid pipe and nozzle etc., and the material commonly using optical glass, quartz etc. transparent, stable is made, and designing and making is all very meticulous, is the heart of liquid fluid system.Sample hose seasoning sample, individual cells suspension penetrates from sample hose under flow stream pressure effect; Sheath fluid flows to spray orifice by sheath fluid pipe from surrounding, penetrates after being enclosed in sample periphery from nozzle.In order to ensure that liquid stream is steady liquid, general restriction flow stream velocity is less than 10m/s.Due to the effect of sheath fluid, detected cell is limited on the axis of liquid stream.Flow chamber is equipped with piezoelectric crystal, and being subject to oscillator signal can vibrate.
Single cell suspension is held the hole by the certain pore size in flow chamber by sheath flow liquid in cell flow chamber, and detection zone is at the center in this hole, and cell is crossing with laser vertical at this, and under sheath flow liquid constraint, cell arranges in single file successively by laser detection district.Sheath fluid stream holds sample stream and makes sample stream remain on the axis direction of liquid stream, can ensure that each cell is equal by the time of laser irradiation area.The single center flowing through this hole of cell, flow velocity is comparatively slow, and thus cell is by long according to the time, and collectable cell signal luminous flux is large, mixes wide-angle collecting lens, can obtain very high detection sensitivity and measuring accuracy.Therefore light signals different for multi-angle is integrated and leucocyte can be divided into five classes.Flow chamber aperture have 60 μm, 100 μm, 150 μm, 250 μm etc. multiple.The miniature instrument general stationary installation flow chamber of certain pore size.
Because the cell volume of test is all at micron order, and need special shape and penetrability when flow chamber receives illumination, therefore small, high-precision flow chamber determines its difficult processing, complex structure and cost is high.At present, when technical requirement is high, domestic being difficult to finds the company that can meet processing request, and foreign technology is ripe, but its processing cost is very high.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, for the flow chamber difficult processing of existing differential hematology analyzer and the high problem of cost, provides the relatively simple and differential hematology analyzer flow chamber that cost is low of a kind of structure.
The technical solution of the utility model is: a kind of blood analyser flow chamber, these both sides, blood analyser flow chamber top are provided with HGB power valve and receiving tube mounting hole, an electrode slice mounting hole is arranged at adjacent HGB power valve bottom, below electrode slice mounting hole, there are a sample port and filter washing water inlet in both sides, a quartz window sheet is respectively had before and after below electrode slice mounting hole, in sample port side and with the coaxial cable of quartz window sheet center on Ku Erte jewel micropore is installed, Ku Erte micropore is processed by jewel sheet, and diameter is 50 μm-250 μm.There is a hemolysin entrance in Ku Erte micropore bottom, below hemolysin entrance, have a removal waste fluid mouth.
The specific works principle of flow chamber is that sample blood and dilution enter in flow chamber from sample and dilution entrance, and injects hemolysin from hemolysin entrance, and mixes.Then launched the light of certain wavelength by the HGB power valve in HGB power valve mounting hole, light, through mixed sample liquid, gathers light signal by the HGB receiving tube in HGB receiving tube mounting hole, draws the HGB parameter of sample.Then the sample liquid in flow chamber enters sample port by Ku Erte jewel micropore, when the micropore that sample liquid passes through, can change the impedance in micropore, be collected the change of impedance by the electrode slice in electrode slice mounting hole, thus record is by the haemocyte number of micropore.At sample liquid by while Ku Erte jewel micropore, another road laser is injected from quartz window, by the micropore at place, be irradiated on the haemocyte by Ku Erte jewel micropore, then by quartz window, be injected into optical signal collection system, form the light signal of cell.When sampling end-of-job after, by will be left after removal waste fluid mixed liquor discharge, simultaneously by filter washing water inlet suction cleaning fluid for cleaning inner chamber.
The feature of differential hematology analyzer flow chamber is its structure is after improving, add the quartz window sheet that 2 are coated with anti-reflection film by the structure of original three grouping blood analyser diluted cups before and after the center of Ku Erte jewel micropore, this quartz window sheet one side plating plating anti-reflection film, the effect of anti-reflection film is the intensity reducing reflected light, thus increase the intensity of transmitted light, make optical system imaging more clear.During installation coated surface dorsad Ku Erte jewel micropore towards the direction of catoptron.The marginal portion of its surrounding is sealed with resin glue, can being fixed on five classification flow chambers of sealing, accomplish the effect of water-proof light permeable.
Ku Erte micro-pore diameter can be 70 μm, 80 μm, 100 μm not etc.
The utility model has the advantage of: flow chamber process structure is relatively simple, without sheath stream pipe, without blast tube and cost is low.The present invention does not adopt traditional flow chamber and diluted cup two parts separately to use, but diluted cup is combined use with flow chamber, electrical impedance detection and optical signal detecting are carried out simultaneously in parts, while saving material, also save sample and reagent, and greatly shorten the working time of instrument.
Accompanying drawing explanation
Fig. 1 is a structural representation of the utility model blood analyser flow chamber;
Fig. 2 is the side structure schematic diagram of the utility model blood analyser flow chamber.
In figure: 100 indicate that sample and dilution entrance, 101 expression HGB receiving tube mounting holes, 102 represent that HGB power valve mounting holes, 103 represent that electrode slice mounting holes, 104 represent that sample ports, 105 represent filter washing water inlets, the special jewel micropore of 106 library representations that, 107 represent that hemolysin entrance, 108 represents that removal waste fluid mouth, 100 indicates that sample and dilution entrance, 104 represent sample port, the special jewel micropore of 106 library representations that, and 108 represent that removal waste fluid mouths, 109 and 110 represent quartz window sheet, arrow represents laser incident direction.
Embodiment
First add sample (blood sample) by 100 to flow chamber, add dilution mixing afterwards, after adding hemolysin by hemolysin entrance 107, have a negative pressure at sample port 104 place the mixed liquor of sample and other solution is extracted out by G from flow chamber, according to Coulter principle, leukocytic DC signal now can be produced; Be combined into the optical system of its design, when focus can be converged in the center of Ku Erte micropore by laser after the light path designed by quartz window sheet 109, the hot spot of convergence will be irradiated to whole Ku Erte micropore simultaneously.When cell passes through from Ku Erte micropore, by generation light scattering signal, quartz window sheet 110 by being coated with anti-reflection film keeps light signal strength unattenuated by this light scattering signal, the light scattering signal of cell converged on the mechanical aperture that diameter is 0.5mm through well-designed light path again, so just make other be not the light scattering signal of tested cell in micropore by mechanical aperture to sheltering from, also spurious signal is just made not affect follow-up light path, the light scattering signal of final tested cell changes into parallel light signal and outputs on photodetector, photodetector will have the change of an electric current thus transmit rear follow-up amplifying circuit.When cell is by micropore, all can produce a light scattering signal in forward and backward, therefore can divide lymphoblast, acidophil, basocyte, monocyte and neutrophil leucocyte by leucocyte in conjunction with DC signal, forward light scattering and rear light scattering signal.
Flow chamber process structure of the present utility model is relatively simple, without the need to sheath flow liquid and cost is low.
The foregoing is only the preferred embodiments of the present invention; not thereby patent of the present invention is limited; every utilize instructions of the present invention and accompanying drawing content to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other association areas, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. a blood analyser flow chamber, it is characterized in that: these both sides, blood analyser flow chamber top are provided with HGB power valve and receiving tube mounting hole, an electrode slice mounting hole is arranged at adjacent HGB power valve bottom, below electrode slice mounting hole, there are a sample port and filter washing water inlet in both sides, a quartz window sheet is respectively had before and after below electrode slice mounting hole, in sample port side and with the coaxial cable of quartz window sheet center on Ku Erte jewel micropore is installed, there is a hemolysin entrance in micropore bottom, below hemolysin entrance, have a removal waste fluid mouth.
2. blood analyser flow chamber according to claim 1, is characterized in that: Ku Erte micropore is processed by jewel sheet, and diameter is 50 μm-250 μm.
3. blood analyser flow chamber according to claim 1, is characterized in that: Ku Erte micro-pore diameter is 100 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420751923.6U CN204255817U (en) | 2014-12-04 | 2014-12-04 | Blood analyser flow chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420751923.6U CN204255817U (en) | 2014-12-04 | 2014-12-04 | Blood analyser flow chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204255817U true CN204255817U (en) | 2015-04-08 |
Family
ID=52960112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420751923.6U Withdrawn - After Issue CN204255817U (en) | 2014-12-04 | 2014-12-04 | Blood analyser flow chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204255817U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
-
2014
- 2014-12-04 CN CN201420751923.6U patent/CN204255817U/en not_active Withdrawn - After Issue
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104458540A (en) * | 2014-12-04 | 2015-03-25 | 南昌百特生物高新技术股份有限公司 | Flow chamber of blood analyzer |
| CN104458540B (en) * | 2014-12-04 | 2017-03-29 | 南昌百特生物高新技术股份有限公司 | Blood analyser flow chamber |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20150408 Effective date of abandoning: 20170329 |
|
| AV01 | Patent right actively abandoned |