CN105717036B - A kind of flow cytometer flow chamber of magnetic stirring - Google Patents
A kind of flow cytometer flow chamber of magnetic stirring Download PDFInfo
- Publication number
- CN105717036B CN105717036B CN201610275628.1A CN201610275628A CN105717036B CN 105717036 B CN105717036 B CN 105717036B CN 201610275628 A CN201610275628 A CN 201610275628A CN 105717036 B CN105717036 B CN 105717036B
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- chamber
- flow
- flow chamber
- sheath
- sheath liquid
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- 238000003760 magnetic stirring Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 67
- 239000012530 fluid Substances 0.000 claims abstract description 47
- 239000011324 bead Substances 0.000 claims abstract description 31
- 230000001681 protective effect Effects 0.000 claims description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims 2
- 239000007921 spray Substances 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 238000013019 agitation Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000005304 optical glass Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1404—Fluid conditioning in flow cytometers, e.g. flow cells; Supply; Control of flow
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- G01N15/1409—
Abstract
The present invention relates to the flow cytometer flow chamber of a kind of flow chamber of flow cytometer, more particularly to a kind of magnetic stirring.Flow chamber and sheath liquid chamber are passed through including flow chamber, sample introduction needle, sheath liquid chamber composition, the sample introduction needle, the sheath liquid chamber is located above flow chamber, is connected by sheath fluid apertures；Magnetic bead is equipped with the flow chamber, electromagnet is equipped between the flow chamber and sheath liquid chamber, the sheath liquid chamber upper end has sheath fluid entrance.Can stably stratified flow quickly, save the energy.When spout sprays, sheath fluid still carries centripetal force, and sample introduction needle is located at circle centre position and typhoon eye position, can ensure that its straight line drops, will not be mixed with sheath fluid, will not block spout.
Description
Technical field
It the utility model is related to a kind of flow chamber of flow cytometer, more particularly to a kind of fluidic cell of magnetic stirring
Instrument flow chamber.
Background technology
Flow cytometer is a kind of very common detecting instrument, forms the flow chamber in its liquid channel system by sample cell, sheath
Liquid pipe and nozzle etc. form, and commonly use the material that optical glass, quartz etc. are transparent, stablize and make.Design and making are very fine, are
The heart of liquid fluid system.Sample cell stores sample, and individual cells suspension projects under flow stream pressure effect from sample cell；Sheath fluid by
Sheath liquid pipe flows to spray orifice from surrounding, is projected after being enclosed in sample periphery from nozzle, ensures that liquid stream is stablized.Due to the effect of sheath fluid,
Detected cell is limited on the axis of liquid stream.Under normal circumstances, sample is injected into the center of sheath flow liquid, sample pressure
Higher than the pressure of sheath flow liquid.And sample liquid flow speed adjusts sample/sheath flow liquid pressure ratio to adjust by pressure regulator.Liquid stream
Speed is accelerated with the increase of sample pressure, at the same sample center is wider and more many cells can once pass through laser detection
Point.On the contrary, flow stream velocity can be slowed down so as to reduce once by the cell number of laser detection point by reducing sample pressure.Flow chamber
Using fluid dynamics focusing principle, sheath stream and sample flow are converged in flow chamber, sample flow is formed stable laminar flow, this
When, sheath stream is enclosed in around sample flow, and sample flow forms stable streamlined flow liquid by laser detection area, for optical system
System detection and analysis, but sheath fluid flowing instability, especially when detection speed is changed, pressure changes, and flow velocity is unstable
It is fixed, and the speed of sheath fluid is generally only adjusted in flow chamber, and sheath liquid chamber is the transition stage of sheath fluid, as flow chamber
The deposit of sheath fluid, but often directly enter when sheath liquid chamber sheath fluid enters flow chamber at the top of flow chamber, it is very easy to cause to flow
Bulk wave moves, it is difficult to forms stable laminar flow, influences experimental result.
CN201520743256.1 discloses a kind of inlet combination device for flow cytometer, which includes
Three trapezoidal chamber sleeve, sheath flow chamber and sample introduction needle structures；The trapezoidal chamber sleeve is equipped with four sheath fluid apertures, sample introduction pin hole one, ladder
Shape chamber and cylindrical cavity.The sheath flow chamber includes two, four annular slotted holes of sample introduction pin hole and flange shape boss.The inlet group
Component uses trapezoidal chamber sleeve design, has larger inlet area, so as to come in the case where not changing sheath stream chamber radius real
Existing big flow sheath fluid flows into.The sheath flow chamber inlet is designed using four annular slotted holes, so as to increase sheath flow quantity, ensures sheath
Liquid stream enters uniformly, avoids turbulent flow.The combination device utilizes plastics counterpart that is cheap and being readily available, the inlet group
Attach together to put and there is simple in structure and user easily assembling and dismounting.
CN201520703127.X is related to a kind of new flow cytometer flow chamber, it mainly includes flow chamber, sample introduction
Pin, upper end cover, sleeve, bottom end cover, revolving fragment, two sheath inflow entrances are arranged at the top of upper end cover, are opened at the top of revolving fragment and sleeve
There is the annular groove of two size and shape all sames, the first sheath flow chamber, the first focal zone, the second sheath stream are provided with flow chamber
Room, the second focal zone.The utility model can form stable laminar flow, avoid the occurrence of turbulent flow, while when sample rate is different
It can keep higher accuracy of detection.
But they do not solve sheath liquid chamber sheath fluid and just start with speed, especially when machine is started shooting in flow chamber
One section of longer time is needed to form stable laminar flow.Continue easily to block spout during sample introduction, or sample pollution flowing
Room.
Utility model content
Problem to be solved：The utility model is rotated using magnetic force causes sheath liquid chamber sheath fluid to have initial velocity, is flowing
Dynamic room causes laminar stable using magnetic agitation, even if changing speed will not have an impact.Especially just opened in machine
When, stably stratified flow, saves the energy quickly.When spout sprays, sheath fluid still carries centripetal force, sample introduction needle be located at circle centre position and
Typhoon eye position, can ensure that its straight line drops, will not be mixed with sheath fluid, will not block spout.
Technical solution：
A kind of flow cytometer flow chamber of magnetic stirring, including flow chamber, sample introduction needle, sheath liquid chamber composition are described
Sample introduction needle passes through flow chamber and sheath liquid chamber, and the sheath liquid chamber is located above flow chamber, is connected by sheath fluid apertures；The flowing
Interior is equipped with magnetic bead, and electromagnet is equipped between the flow chamber and sheath liquid chamber.
Further, the flow chamber point is respectively one Room of flowing, two Room of flowing from top to bottom, flows three Room, institute
Changeover portion wide at the top and narrow at the bottom is equipped between the flow chamber stated.
Further, one Room of flowing, two Room of flowing, three Room of flowing are cylinder, and a chamber cross-section face
Product is less than three Room less than two Room, two chamber cross-section areas.
Further, the magnetic bead is located at the top of flow chamber to be inverted magnetic bead, the magnetic bead and flow chamber activity
Connection.
Further, the sheath fluid chamber interior is equipped with sheath liquid chamber magnetic bead, and the sheath liquid chamber magnetic bead is located at sheath liquid chamber
Center.
Further, the magnetic bead has a through hole for centre.
Further, the electromagnet is divided into sheath liquid chamber electromagnet and flow chamber electromagnet.
Further, there is insulating layer between sheath liquid chamber electromagnet and flow chamber electromagnet.
Further, sheath fluid apertures is using sample introduction needle as the center of circle, every 60 ° of holes, and formation is no less than two concentric circles.
Further, it is equipped with protective cover outside sample introduction needle.
Beneficial effect:
The utility model is creative by the way of magnetic agitation in flow cytometer first, control sheath fluid flow direction and
Flow velocity, breaches conventional sheath fluid flow control mode.Being rotated using magnetic force causes sheath liquid chamber sheath fluid to have initial velocity, is flowing
Room causes laminar stable using magnetic agitation, even if changing speed will not have an impact.Especially when machine has just been opened
Wait, quickly stably stratified flow, save the energy.When nozzle sprays, sheath fluid still carries centripetal force, and sample introduction needle is located at circle centre position and platform
Eye of wind position, can ensure that its straight line drops, will not be mixed with sheath fluid, will not block spout.
The convenient installation of 1 cylinder design and mold, and volume is big, and surrounding is round and smooth, does not hinder, indoor sheath flow velocity is steady
It is fixed.The fluctuation of flow chamber will not be caused, so as to ensure the stabilization of sample flow.Ever-reduced cross-sectional area can be by varying stream
Indoor size is moved to control the size of sheath liquid flow, so as to ensure that the needs to different sample flow sample rates, together
When can keep compared with high measurement accuracy again.
2 inversion magnetic beads be located at flow chamber top, and top movable dismounting and change easy to connect, and account for and occupy a narrow space, because
It is most wide to flow a ceiling portion, space is saved positioned at top.Its speed is adjusted when sheath fluid enters flow chamber, sheath fluid needs shape
Into certain flow direction, increase its power, can farthest reduce the fluctuation of laminar flow.
Sheath fluid chamber interior described in 3 is equipped with sheath liquid chamber magnetic bead, and the sheath liquid chamber magnetic bead is located at sheath liquid chamber center.Sheath liquid chamber
Magnetic bead stirring makes sheath fluid have initial velocity in sheath liquid chamber, increases sheath fluid kinetic energy, reduces the speed difference to flow chamber sheath fluid, reach
The effect of stably stratified flow.It is centrally located so that sheath fluid speed throughout the room is balanced.
Magnetic bead described in 4 has a hole for centre, can surround sample introduction needle and rotate, space-saving to be not required sample introduction needle to change
To reduction casting difficulty.Do not influence overall structure.
5 electromagnet are divided into sheath liquid chamber electromagnet and flow chamber electromagnet, control sheath liquid chamber magnetic bead and flow chamber magnetic bead respectively
Rotating speed.The general setting for being less than flow chamber magnetic bead rotating speed using sheath liquid chamber rotating speed, prevents that sheath liquid chamber rotating speed is high, liquid inertia effect
Do not flow into flow chamber.Two-stage magnetic agitation is reached after accelerating by the flow chamber design of gradient to be continued to accelerate the mesh for forming laminar flow
, save the energy, convenient control.
There is insulating layer between 6 sheath liquid chamber electromagnet and flow chamber electromagnet, prevent from influencing each other and cause Instrument assembly magnetic
Change lowering apparatus precision, instrument service life can be extended.
7 sheath fluid apertures allow sheath fluid to flow into flow chamber under the action of pressure and gravity, play the role of the sheath fluid of supplement, difference
In the mode that conventional flow cytometer two circular cavities or a small number of holes enter by its stream, entered here using multiple hole, raised speed
It hurry up, and be dispersed into the influence reduced to flow chamber laminar flow.
Protective cover is equipped with outside 8 sample introduction needles, electromagnetic interference is prevented, prevents sample introduction needle to be magnetized, allows sample state to be detected
It is intact, do not interfere with detection data precision.
Brief description of the drawings：
Fig. 1 is a kind of flow cytometer flow chamber structure diagram of magnetic stirring；
Fig. 2 is a kind of flow cytometer flow chamber magnetic bead structure diagram of magnetic stirring；
1- sample introduction needles；2- protective covers；3- sheath liquid chambers；4- sheath fluid apertures；5- flows a Room；6- changeover portions；7- flows two Room, 8-
Flow three Room；9- nozzles；10- is inverted magnetic bead；11- electromagnet；12- sheath liquid chamber magnetic beads；13- sheath fluid entrances；14- through holes；15- is exhausted
Edge layer.
Embodiment
Embodiment 1
The flow cytometer flow chamber of a kind of magnetic stirring as shown in Figure 1 and Figure 2, including flow chamber, sample introduction needle 1, sheath fluid
Room 3 forms, and the sample introduction needle 1 passes through flow chamber and sheath liquid chamber 3, and the sheath liquid chamber 3 is located above flow chamber, passes through sheath fluid
Hole 4 connects；Magnetic bead is equipped with the flow chamber, electromagnet 11 is equipped between the flow chamber and sheath liquid chamber 4.
Further, the flow chamber point is respectively one Room 5 of flowing, two Room 7 of flowing from top to bottom, flows three Room 8,
Changeover portion 6 wide at the top and narrow at the bottom is equipped between one Room 5 of flowing, two Room 7, three Room 8.
Further, one Room 5 of flowing, two Room 7 of flowing, three Room 8 of flowing are cylinder, and a Room 5 is transversal
Face area is less than two Room 7；Two Room, 7 cross-sectional area is less than three Room 8.
Further, the magnetic bead is located at the top of flow chamber to be inverted magnetic bead 10, and the magnetic bead is lived with flow chamber
Dynamic connection.
Further, the sheath liquid chamber 3 is internally provided with sheath liquid chamber magnetic bead 12, and the sheath liquid chamber magnetic bead 12 is located at sheath
The center of liquid chamber 3.
Further, the magnetic bead has a through hole 14 for centre.
Further, the electromagnet 11 divides for sheath liquid chamber electromagnet and flow chamber electromagnet.
Further, there is insulating layer 15 between sheath liquid chamber electromagnet and flow chamber electromagnet.
Further, sheath fluid apertures 4 is the center of circle with sample introduction needle 1, every 60 ° of sheath fluid apertures 4, is formed same no less than two
The heart is justified.
Further, the outside of sample introduction needle 1 is equipped with protective cover 2.
Described, sheath liquid chamber top has sheath fluid entrance 13.
Claims (10)
1. the flow cytometer flow chamber of a kind of magnetic stirring, it is characterised in that including flow chamber, sample introduction needle, sheath liquid chamber group
Into the sample introduction needle passes through flow chamber and sheath liquid chamber, and the sheath liquid chamber is located above flow chamber, is connected by sheath fluid apertures；
Magnetic bead is equipped with the flow chamber, electromagnet, sheath liquid chamber upper end tool are equipped between the flow chamber and sheath liquid chamber
There is sheath fluid entrance.
A kind of 2. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that the stream
Dynamic room point is respectively one Room of flowing, two Room of flowing from top to bottom, flows three Room, is equipped between the flow chamber wide at the top and narrow at the bottom
Changeover portion.
A kind of 3. flow cytometer flow chamber of magnetic stirring according to claim 2, it is characterised in that the stream
A dynamic Room, two Room of flowing, three Room of flowing are cylinder, and a chamber cross-section area is small less than two Room, two chamber cross-section areas
In three Room.
A kind of 4. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that the magnetic
Pearl is is inverted magnetic bead, and at the top of flow chamber, the magnetic bead is flexibly connected with flow chamber.
A kind of 5. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that the sheath
Liquid chamber is internally provided with sheath liquid chamber magnetic bead, and the sheath liquid chamber magnetic bead is located at sheath liquid chamber center.
A kind of 6. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that the magnetic
Pearl has a through hole for centre.
A kind of 7. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that the electricity
Magnet is divided into sheath liquid chamber electromagnet and flow chamber electromagnet.
A kind of 8. flow cytometer flow chamber of magnetic stirring according to claim 7, it is characterised in that sheath liquid chamber electricity
There is insulating layer between magnet and flow chamber electromagnet.
A kind of 9. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that sheath fluid apertures with
Sample introduction needle is the center of circle, every 60 ° of holes, and formation is no less than two concentric circles.
A kind of 10. flow cytometer flow chamber of magnetic stirring according to claim 1, it is characterised in that sample introduction needle
Outside is equipped with protective cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610275628.1A CN105717036B (en) | 2016-04-29 | 2016-04-29 | A kind of flow cytometer flow chamber of magnetic stirring |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610275628.1A CN105717036B (en) | 2016-04-29 | 2016-04-29 | A kind of flow cytometer flow chamber of magnetic stirring |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105717036A CN105717036A (en) | 2016-06-29 |
| CN105717036B true CN105717036B (en) | 2018-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610275628.1A Active CN105717036B (en) | 2016-04-29 | 2016-04-29 | A kind of flow cytometer flow chamber of magnetic stirring |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107449716A (en) * | 2017-07-25 | 2017-12-08 | 深圳市孔雀生物科技有限公司 | A kind of sorting unit of flow cytometer |
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| CN204008676U (en) * | 2014-05-26 | 2014-12-10 | 苏州日和科技有限公司 | The full-automatic sample introduction mechanism of a kind of flow cytometer |
| WO2015088673A1 (en) * | 2013-12-13 | 2015-06-18 | H. J. Heinz Company | In–line validator |
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| CN205719895U (en) * | 2016-04-29 | 2016-11-23 | 安徽师范大学 | A kind of flow cytometer flow chamber of magnetic stirring |
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| US8603396B2 (en) * | 2008-08-21 | 2013-12-10 | Xy, Llc | Cell analysis apparatus and methods |
| EP2823285A1 (en) * | 2012-03-09 | 2015-01-14 | Firefly Bioworks, Inc. | Methods and apparatus for classification and quantification of multifunctional objects |
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2016
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|---|---|---|---|---|
| WO2015088673A1 (en) * | 2013-12-13 | 2015-06-18 | H. J. Heinz Company | In–line validator |
| CN203772701U (en) * | 2014-03-03 | 2014-08-13 | 中国科学院苏州生物医学工程技术研究所 | Miniaturized flow cytometry liquid path system |
| CN204008676U (en) * | 2014-05-26 | 2014-12-10 | 苏州日和科技有限公司 | The full-automatic sample introduction mechanism of a kind of flow cytometer |
| CN204630897U (en) * | 2015-05-30 | 2015-09-09 | 广州埃克森生物科技有限公司 | FCM analysis liquid-way system |
| CN205719895U (en) * | 2016-04-29 | 2016-11-23 | 安徽师范大学 | A kind of flow cytometer flow chamber of magnetic stirring |
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