CN102564919A - Flow chamber component, flow chamber blockage removing method and sample analyzer - Google Patents

Flow chamber component, flow chamber blockage removing method and sample analyzer Download PDF

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Publication number
CN102564919A
CN102564919A CN2010106198126A CN201010619812A CN102564919A CN 102564919 A CN102564919 A CN 102564919A CN 2010106198126 A CN2010106198126 A CN 2010106198126A CN 201010619812 A CN201010619812 A CN 201010619812A CN 102564919 A CN102564919 A CN 102564919A
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flow chamber
chamber
liquid
rectification
fluid passage
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CN2010106198126A
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CN102564919B (en
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滕锦
申涛
罗丹丹
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Abstract

The invention discloses a flow chamber component, a flow chamber blockage removing method and a sample analyzer. The flow chamber component comprises a flow chamber, a rectifying chamber, a first adding channel, a second adding channel, liquid adding containers, a first liquid channel, a second liquid channel and liquid containers, wherein the flow chamber is provided with a flow chamber outlet; the rectifying chamber is communicated with the flow chamber; and the rectifying chamber and the flow chamber outlet are respectively connected with the corresponding liquid adding containers by the first adding channel and the second channel, and are respectively connected with the corresponding liquid containers by the first liquid channel and the second liquid channel. When blockage removing is needed for the flow chamber, a liquid enters the flow chamber by the flow chamber outlet, and washes the flow chamber reversely, and the blockage is removed by the rectifying chamber, so that the blockage removing operation and daily maintenance for the flow chamber are convenient.

Description

Flow chamber assembly, flow chamber assembly unimpeded method and sample analyser
Technical field
The invention relates to a kind of flow chamber assembly and sample analyser.
Background technology
In flow cytometer, the flow chamber assembly is the important place of carrying out input.The flow chamber assembly generally comprises flow chamber, specimen needle, rectification chamber, connect the passage of each element and control the operation valve of each passage break-make.The sample flow that detects usefulness is penetrated by specimen needle, through outer field sheath stream parcel, constrains in a narrower and comparatively stable zone in the flow chamber; Middle section like flow chamber; Through the particle in the hot spot irradiation sample flow, collect the various types of scattered light signals that produce, in conjunction with biological property; Signal is carried out statistic of classification, obtain having the parameter information of clinical meaning.
Generally speaking; In order to obtain more stable sample flow and scattered light signal, the latus rectum of flow chamber is less, is generally 0.1~0.4mm; But because the complicated component in sample flow and the sheath fluid; The reactant that blood sample and reagent are arranged, also have bring in the blood collection procedure like foreign material such as skin histology, cotton swab silks, analyser puncture test tube is inhaled appearance and the cap test tube chip that produces or the impurity of other form etc.Therefore, in the process of using flow cytometer, the situation that flow chamber may occur stopping up.
In case flow chamber stops up, comprise little stifledly, sample flow just can not stabilizing effective form, and scattered light signal also distortion can occur, thereby produces the clinical diagnosis information of mistake, in this case, just need safeguard accordingly and gets rid of obstruction flow chamber.
At present, for the maintenance of flow chamber, can automatically cleaning reagent be assigned to the porch of flow chamber by analyser; Through syringe or pressure-driven; Make cleaning reagent get into flow chamber, cleaning reagent can carry out chemical reaction with some specific materials, plays the effect of unimpeded and cleaning.But, for other material, like cap test tube chip etc.; Cleaning reagent can not guarantee to play the effect of chemolysis; And under the situation that occurs stopping up fully at flow chamber, cleaning reagent can not guarantee effectively to enter into the position that flow chamber stops up, therefore; Usually need the artificial instrument such as syringe that uses, push liquid from the Way out of flow chamber and carry out unimpeded.Such unimpeded operation need be opened analyser, and passage that some are relevant or valve disconnection, can carry out, and inconvenience therefore operates.
Summary of the invention
The objective of the invention is deficiency, a kind of flow chamber assembly, unimpeded method and sample analyser of being convenient to flow chamber unimpeded and maintenance is provided to prior art.
For realizing above-mentioned purpose; The present invention has adopted following technical scheme: a kind of flow chamber assembly; Comprise that flow chamber, rectification chamber, first add passage, second and add passage, liquid adding container, first fluid passage, second fluid passage and liquid container; Said flow chamber has the flow chamber outlet, and said rectification chamber and said flow chamber are communicated with, and said rectification chamber adds passage through said first and is connected with said liquid adding container; Outlet is connected with corresponding liquid container with second fluid passage through said first fluid passage respectively with flow chamber in said rectification chamber; When needing unimpeded, make the said first fluid passage conducting, liquid flows into said flow chamber outlet, flow chamber, rectification chamber, first fluid passage reaches and the liquid container of the corresponding connection of first fluid passage.When unimpeded, need utilize the direction of pressure control liquid flow, as this pressure being provided through power source is set, this pressure such as normal pressure or negative pressure, this power source such as syringe or other can provide pressure to drive the power source of liquid flow.
Further, when needing unimpeded, make said first fluid passage and the second fluid passage conducting, liquid flows into said second fluid passage, flow chamber outlet, flow chamber, rectification chamber, first fluid passage reaches and the liquid container of the corresponding connection of first fluid passage.
Further; Described flow chamber assembly comprises that also second adds passage; Said flow chamber outlet adds passage through said second and is connected with said liquid adding container; When needing unimpeded, make said second to add the passage and the first fluid passage conducting, liquid flows into the said second adding passage, flow chamber outlet, flow chamber, rectification chamber, first fluid passage reaches and the liquid container of the corresponding connection of first fluid passage.
Further, said rectification chamber has rectification chamber interface, and the latus rectum of said rectification chamber interface is greater than the latus rectum of said flow chamber, and said first fluid passage connects said rectification chamber interface.Because the latus rectum of rectification chamber interface is bigger, be convenient to discharge the tamper of flow chamber.
Further, the said first adding passage connects said rectification chamber interface.
Further; Said liquid container has two; Be respectively first liquid container and second liquid container, said first liquid container connects said rectification chamber interface through said first fluid passage, and said second liquid container connects said flow chamber outlet through said second fluid passage.
Further, said first liquid container and second liquid container are communicated with.
Further, described flow chamber assembly also comprises specimen needle, and said specimen needle stretches into said rectification chamber and over against said flow chamber.
Further, the said first adding passage, the second adding passage, first fluid passage and second fluid passage are respectively equipped with the first adding controller, second adding controller, first liquid controller and second liquid controller that is used for the control channel break-make.Through controller is set, be convenient to the break-make of each passage is controlled automatically.
Further, in the time of need detecting, make said first to add the passage and the second fluid passage conducting, liquid flows into the said first adding passage, rectification chamber, flow chamber, flow chamber outlet, second fluid passage reaches and the liquid container of the corresponding connection of second fluid passage.
A kind of sample analyser comprises described flow chamber assembly.
A kind of unimpeded method of flow chamber assembly, said flow chamber assembly comprise flow chamber and the rectification chamber that is interconnected, and said flow chamber has the flow chamber outlet, comprises the steps:
A) drive liquid and get into said flow chamber, the tamper that stops up said flow chamber is poured said rectification chamber from said flow chamber outlet; B) driving said tamper discharges from said rectification chamber.
Further, in the said step a), utilize normal pressure to drive said liquid; In the said step b), utilize negative pressure to drive said tamper.
Further, also comprise step c) before the said step a): said flow chamber of emptying and rectification chamber.
Further, also comprise step d) after the said step b): clean, pour into said flow chamber and rectification chamber.
Further; Said step a) comprises the steps: a1) make said rectification chamber be connected liquid adding container through the first adding passage and the second adding passage respectively with the flow chamber outlet, make said flow chamber outlet be connected corresponding liquid container with second fluid passage through first fluid passage respectively with the rectification chamber; A2) make said second to add the passage and the first fluid passage conducting, utilize normal pressure to drive said liquid and add the interior liquid of container from the said flow chamber of said flow chamber outlet entering; In the said step b), make said first fluid passage and the second fluid passage conducting, utilize negative pressure to drive tamper and discharge from said rectification chamber.
The invention has the beneficial effects as follows: in the time of need be to the flow chamber unimpeded, make liquid pass through the flow chamber outlet and get into flow chamber, flow chamber is carried out counterflush, and discharge tamper, made things convenient for the unimpeded of flow chamber is operated and daily maintaining through the rectification chamber.
Description of drawings
Fig. 1 is the structural representation of flow chamber assembly first embodiment;
Fig. 2 is the structural representation of flow chamber assembly second embodiment.
Embodiment
Combine accompanying drawing that the present invention is done further explain through embodiment below.
As shown in Figure 1, this embodiment flow chamber component application is in sample analyser, and it comprises that flow chamber 1, rectification chamber 2, specimen needle 3, liquid add container 4, first liquid container 6 and second liquid container 5.Flow chamber 1 has flow chamber inlet 11 and flow chamber outlet 12.Rectification chamber 2 is communicated with flow chamber inlet 11, and this rectification chamber 2 has rectification chamber interface 201.Specimen needle 3 stretches into rectification chamber 2 and over against flow chamber 1.Liquid adds container 4 and is connected with rectification chamber interface 201 through the first adding passage 7, and this first adding passage 7 is provided with the first adding controller 43 that is used for the control channel break-make.Liquid adding container 4 adds passage 8 through second and is connected with flow chamber outlet 12, and this second adding passage 8 is provided with the second adding controller 44 that is used for the control channel break-make.First liquid container 6 is connected with rectification chamber interface 201 through first fluid passage 9, and this first fluid passage 9 is provided with first liquid controller 42 that is used for the control channel break-make.Second liquid container 5 is connected with flow chamber outlet 12 through second fluid passage 10, and this second fluid passage 10 is provided with second liquid controller 45 that is used for the control channel break-make.
When carrying out pattern detection, can open first and add the controller 43 and second liquid controller 45, the reagent in the liquid adding container 4 drive through normal pressure, and interface 201 gets into rectification chamber 2 from the rectification chamber, and in rectification chamber 2 and flow chamber 1, forms stable laminar flow; Sample to be tested adds rectification chamber 2 from specimen needle 3, after 2 rectifications of overcommutation chamber, flow into flow chamber 1, forms the stable sample flow by the laminar flow parcel.Sample flow is to be detected through flow chamber 1 time, and the waste liquid that detects after accomplishing exports 12 discharges and is collected into second liquid container 5 from flow chamber, and can be discharged to outside the analyser through second liquid container 5.Certainly, also can first liquid container 6 and second liquid container 5 be communicated with, unified by first liquid container, 6 collection waste liquids; Collect a certain amount of after; Under normal pressure drives, the waste liquid in first liquid container is discharged to outside the analyser, in such cases; Analyser can only be provided with a waste liquid mouth, is convenient to control.
When flow chamber 1 is blocked when needing unimpeded, at first open first liquid controller 42 and second liquid controller 45, with flow chamber 1 and 2 emptyings of rectification chamber; Then; Close second liquid controller 45 and open second and add controller 44; Add at liquid under the negative pressure effect of normal pressure and first liquid container 6 of container 4; Liquid adds passage 8 along second and gets into flow chamber 1 through flow chamber outlet 12, and flow chamber 1 is carried out counterflush, and the liquid after the flushing is discharged to first liquid container 6 through rectification chamber interface 201; Then close second and add controller 44 and open second liquid controller 45, under the negative pressure effect of first liquid container 6,, the tamper that develops is discharged to first liquid container 6 through rectification chamber interface 201 2 emptyings of rectification chamber; At last.Clean, pour into flow chamber 1 and rectification chamber 2, whole flow chamber assembly is resetted.
In the time of need cleaning the passage (like second fluid passage) in flow chamber exit and controller (like second liquid controller); Can directly open and add the second adding controller 44 and second liquid controller 45; The liquid that directly utilizes liquid to add in the container washes this passage and controller; Need not pass through flow chamber, realize the direct cleaning to the flow chamber exit, the waste liquid that has reduced in passage and the controller is residual.
Rectification chamber 2 can have accelerating sections 202, and rectification chamber 2 is connected with flow chamber inlet 11 through this accelerating sections 202, can be accelerated during this accelerating sections of sample flow process.Accelerating sections 202 can be taper, and the less end of the latus rectum of this accelerating sections 202 is connected with flow chamber inlet 11.Specimen needle 3 can add passage 13 with sample and be connected.Sample adds passage 13 can connect sample container and sample driving power source; This sample adds passage 13 can connect this specimen needle through three-port structure, being connected outlet 302 1 sides that drive power source controller 41,46 all can be set of inlet 301 1 sides of the connection sample container of three-port structure and three-port structure; Certainly, sample adds the structure that passage also can be under power drive be transported to sample specimen needle 3 for other.
As shown in Figure 2; It is second embodiment of flow chamber assembly; This embodiment is with the key distinction of first embodiment: the second adding passage that is connected flow chamber outlet 12 and liquid adding container is not set, and other structure of this embodiment can be identical with first embodiment.
During cleaning, make first to add passage 7 and 10 conductings of second fluid passage, liquid can flow into second and add passage 7, rectification chamber 2, flow chamber 1, flow chamber outlet 12, second fluid passage 10 and second liquid container 5.During unimpeded; Make first fluid passage 9 and 10 conductings of second fluid passage; To the pressurization of second liquid container 5, under pressure, liquid can flow into second fluid passage 10, flow chamber outlet 12, flow chamber 1, rectification chamber 2, first fluid passage 9 and first liquid container 6.
For the flow chamber assembly, comprise flow chamber and rectification chamber, this flow chamber has the flow chamber outlet; This rectification chamber is communicated with this flow chamber, and outlet all is connected with corresponding liquid container with flow chamber in the rectification chamber, and the rectification chamber also is connected with liquid adding container; When needing to detect; Under pressure-driven, liquid add flow of liquid in the container through the rectification chamber, flow chamber and flow chamber outlet, the liquid container collection that is connected with this flow chamber outlet of quilt at last; When needing unimpeded, under pressure-driven, flow of liquid is through flow chamber outlet, flow chamber and rectification chamber, and the liquid container that is connected with this rectification chamber of quilt is collected at last.When needing unimpeded; Liquid gets into flow chamber from the flow chamber outlet; Realization is to the counterflush of flow chamber; This liquid can be to add container output by the liquid that is connected with the flow chamber outlet, also can be the liquid container output that is connected by with the flow chamber outlet, or by other container output that is connected with the flow chamber outlet.
For the flow chamber assembly, the flow chamber outlet all can be connected liquid adding container through adding passage with the rectification chamber.Liquid adds container can only have one, and it adds passages through two and realizes respectively and being connected of flow chamber outlet and rectification chamber; Liquid adds container also can have two, and these two liquid add container and connect flow chamber outlet and rectification chamber respectively through two adding passages.Liquid container can have two, and these two liquid containers connect rectification chamber and flow chamber outlet respectively through two fluid passages, and these two liquid containers can independently be provided with, and also can pass through channel connection; Liquid container also can only have one, i.e. flow chamber outlet is connected this liquid container through fluid passage respectively with the rectification chamber.The rectification chamber can have a rectification chamber interface, and promptly this rectification chamber interface can connect first liquid container and liquid adding container simultaneously; The rectification chamber also can have two rectification chamber interfaces, and two rectification chamber interfaces connect first liquid container and liquid adding container respectively.The latus rectum of rectification chamber interface can be greater than the latus rectum of flow chamber, thereby is convenient to discharge tamper, and this latus rectum is meant the size that the inside of this interface or flow chamber can be passed through for liquid.All can be provided for the controller of control channel break-make on each passage, this controller such as solenoid valve, pneumatic pilot valve, perhaps other can play the structure of passage break-make control action.
The flow chamber assembly can be applied on the flow cytometer, on blood cell analyzer, perhaps other flow chamber and rectification chamber need be set and sample analyser that the sample flow through flow chamber is detected on.
A kind of unimpeded method of flow chamber assembly; The flow chamber assembly comprises flow chamber and the rectification chamber that is interconnected; Flow chamber has the flow chamber outlet, comprises the steps: a) to drive liquid and flows into flow chamber from the flow chamber outlet, and the tamper that stops up said flow chamber is poured the rectification chamber; B) driving tamper discharges from the rectification chamber.For the ease of fully washing tamper, this method can also be positioned at the step c) before the said step a): with flow chamber and the emptying of rectification chamber.For the ease of carrying out detecting operation next time, this method can also comprise the step d) that is positioned at after the said step b): clean, perfusion flow chamber and rectification chamber, whole flow chamber assembly is resetted.
Step a) can comprise: a1) make the rectification chamber add passage through the first adding passage and second respectively with the flow chamber outlet and be connected liquid adding container, make the flow chamber outlet be connected corresponding liquid container with second fluid passage through first fluid passage respectively with the rectification chamber; A2) make second to add passage and the equal conducting of first fluid passage, the normal pressure of utilizing liquid to add container drives liquid and gets into flow chamber from the flow chamber outlet; In the step b), make first fluid passage and the equal conducting of second fluid passage, utilize the negative pressure driving tamper of the liquid container that is connected with the rectification chamber to discharge from the rectification chamber.
Step a) also can comprise: a1) make the rectification chamber add passage through first and connect liquid adding container, make the flow chamber outlet be connected corresponding liquid container with second fluid passage through first fluid passage respectively with the rectification chamber; A2) make first fluid passage and the equal conducting of second fluid passage, utilize the normal pressure of the liquid container that is connected with the flow chamber outlet to drive liquid from flow chamber outlet entering flow chamber; In the said step b), utilize the negative pressure driving tamper of the liquid container that is connected with the rectification chamber to discharge from the rectification chamber.
Above content is to combine concrete embodiment to the further explain that the present invention did, and can not assert that practical implementation of the present invention is confined to these explanations.For the those of ordinary skill of technical field under the present invention, under the prerequisite that does not break away from the present invention's design, can also make some simple deduction or replace, all should be regarded as belonging to protection scope of the present invention.

Claims (15)

1. flow chamber assembly; Comprise flow chamber and rectification chamber; Said flow chamber has the flow chamber outlet; Said rectification chamber and said flow chamber are communicated with, and it is characterized in that: comprise that also first adds passage, liquid adding container, first fluid passage, second fluid passage and liquid container, said rectification chamber adds container through the said first adding passage with said liquid and is connected; Outlet is connected with corresponding liquid container with second fluid passage through said first fluid passage respectively with flow chamber in said rectification chamber; When needing unimpeded, make the said first fluid passage conducting, liquid flows into said flow chamber outlet, flow chamber, rectification chamber, first fluid passage reaches and the liquid container of the corresponding connection of first fluid passage.
2. flow chamber assembly as claimed in claim 1; It is characterized in that: when needing unimpeded; Make said first fluid passage and the equal conducting of second fluid passage, liquid flows into said second fluid passage, flow chamber outlet, flow chamber, rectification chamber, first fluid passage reaches and the liquid container of the corresponding connection of first fluid passage.
3. flow chamber assembly as claimed in claim 1; It is characterized in that: comprise that also second adds passage; Said flow chamber outlet adds passage through said second and is connected with said liquid adding container; When needing unimpeded, make said second to add passage and the equal conducting of first fluid passage, liquid flows into the said second adding passage, flow chamber outlet, flow chamber, rectification chamber, first fluid passage reaches and the liquid container of the corresponding connection of first fluid passage.
4. like any described flow chamber assembly among the claim 1-3, it is characterized in that: said rectification chamber has rectification chamber interface, and the latus rectum of said rectification chamber interface is greater than the latus rectum of said flow chamber, and said first fluid passage connects said rectification chamber interface.
5. flow chamber assembly as claimed in claim 4 is characterized in that: said first adds passage connects said rectification chamber interface.
6. flow chamber assembly as claimed in claim 4; It is characterized in that: said liquid container has two; Be respectively first liquid container and second liquid container; Said first liquid container connects said rectification chamber interface through said first fluid passage, and said second liquid container connects said flow chamber outlet through said second fluid passage.
7. flow chamber assembly as claimed in claim 6 is characterized in that: said first liquid container and second liquid container are communicated with.
8. like any described flow chamber assembly among the claim 1-3, it is characterized in that: also comprise specimen needle, said specimen needle stretches into said rectification chamber and over against said flow chamber.
9. like any described flow chamber assembly among the claim 1-3, it is characterized in that: each passage is equipped with the controller that is used for the control channel break-make.
10. a sample analyser is characterized in that: comprise any described flow chamber assembly among the claim 1-9.
11. the unimpeded method of a flow chamber assembly, said flow chamber assembly comprise flow chamber and the rectification chamber that is interconnected, said flow chamber has the flow chamber outlet, it is characterized in that: comprise the steps:
A) drive liquid and get into said flow chamber, the tamper that stops up said flow chamber is poured said rectification chamber from said flow chamber outlet;
B) driving said tamper discharges from said rectification chamber.
12. the unimpeded method of flow chamber assembly as claimed in claim 11 is characterized in that: in the said step a), utilize normal pressure to drive said liquid; In the said step b), utilize negative pressure to drive said tamper.
13. the unimpeded method of flow chamber assembly as claimed in claim 11 is characterized in that: also comprise step c) before the said step a): said flow chamber of emptying and rectification chamber.
14. the unimpeded method of flow chamber assembly as claimed in claim 13 is characterized in that: also comprise step d) after the said step b): clean, pour into said flow chamber and rectification chamber.
15. the unimpeded method like any described flow chamber assembly among the claim 11-14 is characterized in that: said step a) comprises the steps:
A1) make said rectification chamber add passage through the first adding passage and second respectively and be connected liquid adding container, make said flow chamber outlet be connected corresponding liquid container with second fluid passage through first fluid passage respectively with the rectification chamber with the flow chamber outlet;
A2) make said second to add passage and the equal conducting of first fluid passage, utilize normal pressure to drive said liquid and add the interior liquid of container from the said flow chamber of said flow chamber outlet entering;
In the said step b), make said first fluid passage and the equal conducting of second fluid passage, utilize negative pressure to drive tamper and discharge from said rectification chamber.
CN201010619812.6A 2010-12-31 2010-12-31 Flow chamber component, flow chamber blockage removing method and sample analyzer Active CN102564919B (en)

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Application publication date: 20120711

Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd.

Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd.

Contract record no.: X2022440020009

Denomination of invention: Flow chamber assembly, blockage removal method for flow chamber assembly, and sample analyzer

Granted publication date: 20140319

License type: Common License

Record date: 20220804