CN205898639U - Clear stifled device of flow cytometer - Google Patents
Clear stifled device of flow cytometer Download PDFInfo
- Publication number
- CN205898639U CN205898639U CN201620879032.8U CN201620879032U CN205898639U CN 205898639 U CN205898639 U CN 205898639U CN 201620879032 U CN201620879032 U CN 201620879032U CN 205898639 U CN205898639 U CN 205898639U
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- CN
- China
- Prior art keywords
- flow cytometer
- way valve
- syringe
- blockage
- connecting tube
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model belongs to the technical field of it is biological, concretely relates to clear stifled device of flow cytometer, it includes the device main part, the device main part is including the three -way valve, connecting pipe and the syringe that connect gradually, between three -way valve and the connecting pipe, all be connected through the retainer plate between connecting pipe and the syringe, the three -way valve is two three -way three -way valves. The utility model discloses can be clear stifled to flow cytometer effectively, can use to flow cytometer by the direct mount, need not reform transform flow cytometer, clear stifled convenient operation, swift.
Description
Technical field
This utility model belongs to biological technical field and in particular to a kind of flow cytometer blockage-clearing device.
Background technology
Flow cytometry (flowcytometry, fcm) is a kind of extremely important modern biology technology, for outstanding
Float on the molecule in fluid (inclusion cell) to be analyzed and sort, be widely used to scientific research and medical science inspection at present
Survey.Flow cytometry needs to use flow cytometer and sheath fluid；Its principle is each particle by high speed sheath liquor stream band
Dynamic, by can be in some way light scattering during laser beam, the fluorescent chemicalses simultaneously being carried be excited and launch frequency
Rate is less than the fluorescence of exciting light.The data splitting of these scattered lights and fluorescence is by detector record it becomes possible to extrapolate each
The physics of grain and chemical property.
Flow cytometry device is more complicated, accurate.The diameter that the quartz flow of general flow cytometer is indoor is less than 100
Micron, the aperture of sample introduction needle are less than 400 microns；So the quartz flow room of flow cytometer is often blocked up by various objects in reality
Plug；Such as it is deposited the blocking such as thing, AC.Flow cytometer blocking needs to stop experiment, runs the clear of flow cytometer
Reason function.The principle of the clearing function of flow cytometer is to replace sample with cleanout fluid, and drives cleaning with high speed sheath liquor stream
Liquid is through sample introduction needle and quartz flow room, thus clearing up sample introduction needle and quartz flow room.The scale removal process of flow cytometer is general
Need 20 minutes to 2 hours, and a large amount of sheath fluids and cleanout fluid can be consumed.This cleanout fluid is passively with sheath stream forms
It is rinsed use, cleaning effect is bad, flow cytometer blocking then cannot be cleared up when more serious, need the engineer of specialty to process.
Utility model content
For solving the above problems, the purpose of this utility model is to provide a kind of flow cytometer blockage-clearing device, Ke Yiyou
Effect, quickly and easily flow cytometer block clearing.
For achieving the above object, syringe is passed through the stone of connecting tube and three-way valve and flow cytometer by this utility model
English flow chamber connects, and cooperation water check valve, the artificial pull syringe of dependence can achieve flow cytometer block clearing effectively；Threeway
Valve is the three-way valve of two-bit triplet, can quickly and easily three ports of switching three-way valve as needed UNICOM's state.
The detailed technology scheme that this utility model is taken is: a kind of flow cytometer blockage-clearing device, and it includes apparatus main body,
Apparatus main body includes three-way valve, connecting tube and the syringe being sequentially connected；Between three-way valve and connecting tube, connecting tube and injection
All connected by ring for fixing between device；Three-way valve is the three-way valve of two-bit triplet.
Further, connecting tube is silica gel tube.Cushioning effect can be played, it is to avoid the strength of pull syringe is excessive and damages
Bad flow cytometer.
Further, ring for fixing carries the rubber ring of annular projection for inwall.The sealing of ring for fixing junction can be improved
Property.
Further, three-way valve is t type three-way valve.
Further, syringe is injector for medical purpose.
Compared with prior art, this utility model have the advantages that 1, can effectively flow cytometer clear
Stifled；2nd, can be mounted directly on flow cytometer using it is not necessary to flow cytometer is transformed；3rd, block clearing operation is compared
Convenient, fast.
Brief description
Fig. 1 is the structural representation of one of this utility model flow cytometer blockage-clearing device.
Fig. 2 is schematic diagram when one of this utility model flow cytometer blockage-clearing device uses.
Apparatus main body 1；Three-way valve 11；Connecting tube 12；Ring for fixing 13；Syringe 14；Flow cytometer 2；Sample introduction needle 21；Enter
Sample mouth 22；Sample export 23；Sheath fluid entrance 24；Sheath fluid outlet 25；Water check valve 3；Threeway valve opening a；Threeway valve opening b；Three
Port valve port c.
Specific embodiment
Refer to Fig. 1, Fig. 2, this utility model is a kind of flow cytometer blockage-clearing device, and it includes apparatus main body 1, device
Main body 1 includes three-way valve 11, connecting tube 12 and the syringe 14 being sequentially connected；Between three-way valve 11 and connecting tube 12, it is connected
All it is connected by ring for fixing 13 between pipe 12 and syringe 14；Three-way valve 11 is the three-way valve of two-bit triplet.
The operation principle of one of this utility model flow cytometer blockage-clearing device is: when preparing to use, by three-way valve
Port a is connected by ring for fixing 13 with sample export 23；Then water check valve 3 being arranged on sheath fluid entrance 24 makes sheath fluid can only enter
(direction that i.e. water check valve 3 permission liquid passes through is identical with the direction that sheath fluid enters sheath fluid entrance 24) can not be gone out；By unidirectional water
Valve 3 is arranged on sheath fluid outlet 25 makes sheath fluid can only go out to enter (direction that i.e. water check valve 3 permission liquid passes through and sheath fluid outflow
The direction of sheath fluid outlet 25 is identical).
When flow cytometer is normal (when there is not blocking), adjustment three-way valve 11 makes threeway valve opening a lead to three-way valve
Mouth b (now threeway valve opening c is automatically switched off), sample enters the quartz stream of flow cytometer 2 from the injection port 22 of sample introduction needle 21
Dynamic room, sheath fluid enters flow cytometer 2 from sheath fluid entrance 24；High speed sheath liquor stream drives sample to form individual particle sample stream, in stream
In formula cell instrument 2 after laser detection；Sample flows into three-way valve 11 and from threeway valve opening b outflow from sample export 23；Sheath
Liquid is from sheath fluid outlet 25 outflow.By above-mentioned it can be seen that flow cytometer can normally use.
When the quartz flow room of flow cytometer occurs blocking, syringe 14 is taken off and sucks cleaning liquid (such as 70%
Ethanol), syringe 14 is passed through ring for fixing 13 and connecting tube 12 UNICOM；Then adjustment three-way valve 11 make threeway valve opening a with
Threeway valve opening c UNICOM (now threeway valve opening b is automatically switched off), reciprocating sliding syringe 14 can clear up quartz flow room；
Waste liquid produced by cleaning (i.e. the mixture of sheath fluid and cleaning liquid) is from sheath fluid sheath fluid outlet 25 outflow.
When the sample introduction needle 21 of flow cytometer occurs blocking, syringe 14 and connecting tube 12 are taken off and suck cleaning liquid
(such as 70% ethanol), connecting tube 12 is passed through ring for fixing 13 and injection port 22 UNICOM, and then adjustment three-way valve 11 makes threeway
Valve opening a and threeway valve opening b UNICOM (now threeway valve opening c is automatically switched off), reciprocating sliding syringe 14 can clear up into
Sample pin 21；Waste liquid produced by cleaning (i.e. the mixture of sheath fluid and cleaning liquid) is from sheath fluid sheath fluid outlet 25 outflow.
From the above, it is seen that one of this utility model flow cytometer blockage-clearing device, can convection type effectively
Cell instrument block clearing；Can to be mounted directly on flow cytometer using it is not necessary to flow cytometer is transformed；Block clearing operates
More convenient, quick.
Connecting tube 12 can be silica gel tube.Cushioning effect can be played, it is to avoid the strength of pull syringe 14 is excessive and damages
Bad flow cytometer.
Ring for fixing 13 can have the rubber ring of annular projection for inner wall belt.The sealing of ring for fixing 13 junction can be improved
Property.
Three-way valve 11 can be t type three-way valve.
Syringe 14 can be injector for medical purpose.
In sum, one of this utility model flow cytometer blockage-clearing device, can flow cytometer effectively
Block clearing；Can to be mounted directly on flow cytometer using it is not necessary to flow cytometer is transformed；Block clearing operation side
Just, fast.
It is to be appreciated that term " " center ", " longitudinal ", " horizontal ", "front", "rear", "left", "right", " vertical ", " water
Flat ", " top ", " bottom ", " interior ", the orientation of instruction such as " outward " or position relationship be based on orientation shown in the drawings or position relationship,
It is only for ease of the description present invention and to simplify description, rather than instruction or infer the device of indication or element must have specifically
Orientation, be specific azimuth configuration and operation, thus it is not intended that to the present invention protect content restriction.
Above specific embodiment is only the preferred embodiment of this creation, not in order to limit this creation, all in this wound
Any modification, equivalent substitution and improvement of being done etc. within the spirit made and principle, should be included in this creation protection domain it
Interior.
Claims (5)
1. a kind of flow cytometer blockage-clearing device it is characterised in that: include apparatus main body (1), apparatus main body (1) include connect successively
Three-way valve (11), connecting tube (12) and the syringe (14) connecing；Between three-way valve (11) and connecting tube (12), connecting tube (12)
All it is connected by ring for fixing (13) between syringe (14)；Three-way valve (11) is the three-way valve of two-bit triplet.
2. as claimed in claim 1 a kind of flow cytometer blockage-clearing device it is characterised in that: described connecting tube (12) be silica gel
Pipe.
3. as claimed in claim 1 a kind of flow cytometer blockage-clearing device it is characterised in that: described ring for fixing (13) be inwall
Rubber ring with annular projection.
4. described a kind of flow cytometer blockage-clearing device as arbitrary in claim 1-3 it is characterised in that: described three-way valve (11)
For t type three-way valve.
5. described a kind of flow cytometer blockage-clearing device as arbitrary in claim 1-3 it is characterised in that: described syringe (14)
For injector for medical purpose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620879032.8U CN205898639U (en) | 2016-08-14 | 2016-08-14 | Clear stifled device of flow cytometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620879032.8U CN205898639U (en) | 2016-08-14 | 2016-08-14 | Clear stifled device of flow cytometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205898639U true CN205898639U (en) | 2017-01-18 |
Family
ID=57782056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620879032.8U Expired - Fee Related CN205898639U (en) | 2016-08-14 | 2016-08-14 | Clear stifled device of flow cytometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205898639U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110270564A (en) * | 2018-03-15 | 2019-09-24 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of cleaning method and sample analyser of reactant |
-
2016
- 2016-08-14 CN CN201620879032.8U patent/CN205898639U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110270564A (en) * | 2018-03-15 | 2019-09-24 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of cleaning method and sample analyser of reactant |
| CN110270564B (en) * | 2018-03-15 | 2022-06-10 | 深圳迈瑞生物医疗电子股份有限公司 | Reactant cleaning method and sample analyzer |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200730 Address after: Unit 601, block 1, No. 116, Gulou Road, Gulou District, Fuzhou City, Fujian Province Patentee after: Gui Jinpeng Address before: 100041 Beijing, Shijingshan District, West Wells Road, No. 19, building 6, floor 3, 601 Patentee before: BEIJING IVY BIOMED MEDICAL SCIENCE TECHNOLOGY Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170118 Termination date: 20200814 |