CN218872250U - Micro-fluidic chip reagent filling instrument - Google Patents
Micro-fluidic chip reagent filling instrument Download PDFInfo
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- CN218872250U CN218872250U CN202223034480.2U CN202223034480U CN218872250U CN 218872250 U CN218872250 U CN 218872250U CN 202223034480 U CN202223034480 U CN 202223034480U CN 218872250 U CN218872250 U CN 218872250U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The utility model provides a micro-fluidic chip reagent filling appearance belongs to reagent filling technical field, and manually operation that aim at solved prior art existence is consuming time power, the precision is low and current filling system operation is complicated, influence the problem of test result. The utility model discloses a: the chip rotary table is used for placing the blood sample chip on the chip rotary table, the blood sample chip is driven to rotate by the chip rotary table, and a plurality of reaction chambers are uniformly distributed on the circumference of the blood sample chip; the liquid injection unit comprises a liquid injection needle support, a plurality of liquid injection needles which are uniformly distributed on the liquid injection needle support and penetrate through the upper surface and the lower surface of the liquid injection needle support, and a liquid conveying unit for conveying liquid to the liquid injection needles quantitatively; the height adjusting unit drives the liquid injection needle bracket of the liquid injection unit to move up and down, and adjusts the relative distance of the liquid outlet end of the liquid injection needle relative to the reaction cavity of the blood sample chip; the display control unit controls the chip turntable, the liquid injection unit and the height adjusting unit to act and the power supply to supply power for the whole body.
Description
Technical Field
The utility model belongs to the technical field of the reagent filling, concretely relates to micro-fluidic chip reagent filling instrument.
Background
The micro-fluidic chip integrates basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a chip with a micro-scale structure, adopts the electrowetting technical principle, regulates and controls the surface energy of solid and liquid by electric potential, and drives the liquid to move by utilizing the unbalanced state of the surface energy, thereby achieving the accurate control of micro-liquid. The technology can integrate operation processes frequently needed in the fields of biology, chemistry, medicine and the like, such as sampling, dilution, reagent adding, reaction, separation, detection and the like, on a digital microfluidic chip, and compared with the traditional control means, the technology can realize less sample consumption, and has the advantages of high sensitivity, high accuracy, high flux, high integration degree and the like, can quickly realize full-flow automatic integration of biochemical reaction with lower cost, and is totally closed without cross contamination in the full-flow reaction, so that the technology can be operated by one key, and the hands of operators are greatly liberated.
In the liquid injection process of the microfluidic chip, an operator is usually required to use a liquid transfer gun to absorb a certain amount of liquid samples and align the liquid samples to a sample inlet, so that the liquid is completely injected into the reaction cavity, but the use cost is increased by using the liquid transfer gun to inject the samples, and the operation accuracy of the operator is higher. Manual operation is time-consuming and labor-consuming, low in accuracy and easy to make mistakes. In addition, the sample injection mode of the traditional technology generally needs a pipette or an external mechanical pump, so that the manufacturing cost is high, the operation flow is complex, the use environment is limited, the steps of the liquid sample injection process are complicated, and waste and misoperation are easily caused.
Chinese patent publication No. CN207210432U discloses a technical scheme of an automatic reagent filling system, which includes: a reagent receiving chamber, a reagent inlet of the reagent receiving chamber being in fluid communication with a reagent outlet of the reagent storage device, a reagent outlet of the reagent receiving chamber being in fluid communication with a reagent inlet of the chip; the automatic pushing device is connected with the reagent accommodating chamber and can add the reagent in the reagent storage device into the reagent accommodating chamber and fill the reagent in the reagent accommodating chamber onto the chip; wherein, be provided with first fluid on-off valve between the reagent entry of reagent accommodation chamber and the reagent export of reagent storage device, be provided with the second fluid on-off valve between the reagent export of reagent accommodation chamber and the reagent entry of chip. However, the filling system can only fill the fixed-cavity reagent, the chip replacement is troublesome, the operation is complex, and the problem that the test result is influenced by sample mixing is easily caused.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a micro-fluidic chip reagent filling appearance solves the problem that manual operation that prior art exists is consuming time power, the precision is low and current filling system operation is complicated, influence test result.
In order to achieve the above object, the utility model discloses a micro-fluidic chip reagent filling instrument includes:
the chip rotary table is used for placing the blood sample chip on the chip rotary table, the blood sample chip is driven to rotate by the chip rotary table, and a plurality of reaction chambers are uniformly distributed on the circumference of the blood sample chip;
the liquid injection unit comprises a liquid injection needle support, a plurality of liquid injection needles which are uniformly distributed on the liquid injection needle support and penetrate through the upper surface and the lower surface of the liquid injection needle support, and a liquid conveying unit for conveying liquid to the liquid injection needles in a quantitative mode;
the height adjusting unit drives the liquid injection needle bracket of the liquid injection unit to move up and down, and adjusts the relative distance between the liquid outlet end of the liquid injection needle and the reaction cavity of the blood sample chip;
the display control unit controls the chip turntable, the liquid injection unit and the height adjusting unit to act;
and the power supply supplies power to the chip turntable, the height adjusting unit and the display control unit.
The liquid conveying unit comprises a plurality of groups, each group comprises a peristaltic pump and a liquid injection pipe, one end of the liquid injection pipe is communicated with a liquid storage bottle for storing liquid, and the other end of the liquid injection pipe penetrates through a pump head of the peristaltic pump and a liquid inlet end of a corresponding liquid injection needle to be connected.
The height adjusting unit comprises a lead screw module and a driving motor connected to one end of a lead screw of the lead screw module, a nut of the lead screw module is fixedly connected with a liquid injection needle support of the liquid injection unit, and the motor drives the nut and the liquid injection needle support to move up and down.
The display control unit comprises a peristaltic pump controller, a programmable controller, a motor controller and a red-green indicator light; the peristaltic pump controller controls the peristaltic pump to act, and the motor controller controls the height adjusting unit and the chip rotary table to act; the red and green indicator light is controlled to be bright or dark through a programmable controller.
The filling instrument further comprises a box body shell, and the liquid injection unit and the height adjusting unit are positioned in the box body shell; the chip turntable is arranged on the upper end face of the bottom plate of the box body shell; and a driving motor of the height adjusting unit is fixed on the box body shell, and drives the screw nut to rotate relative to the screw rod and to do linear motion along the guide rod.
The number of the reaction cavities of the blood sample chip is integral multiple of the number of the liquid injection needles of the liquid injection unit.
The utility model has the advantages that: the utility model discloses a micro-fluidic chip reagent adds appearance adopts peristaltic pump controller control peristaltic pump, is annotated the antibody reagent through annotating the liquid pipe and annotating the liquid needle by the peristaltic pump, pours into in the blood sample chip. The liquid injection needles of the liquid injection unit comprise a plurality of reaction cavities which are adjacent to each other and have the same number, so that the plurality of reaction cavities can be injected at one time, the blood sample chip is driven to rotate by the chip rotary table, the next group of reaction cavities can be adjusted to be in one-to-one correspondence with the liquid injection needles, the height of the liquid injection needle support is adjusted by the height adjusting unit before and after adjustment, avoidance is realized, and the liquid injection needles are prevented from being damaged by interference with the blood sample chip; the invention automatically and accurately adds the reagent through the numerical control operation interface, and has the characteristics of high precision, simple operation and the like. In order to facilitate a user to comprehensively know the working condition of the reagent filling device, the device can display and check information such as the working state of the peristaltic pump on a touch screen of the peristaltic pump controller in real time, and can also import data through the programmable controller to realize operations such as device operation.
Drawings
Fig. 1 is a schematic view of the whole structure of the microfluidic chip reagent filling instrument of the present invention;
fig. 2 is a schematic structural view of the hidden case casing of the microfluidic chip reagent filling instrument of the present invention;
FIG. 3 is a schematic diagram showing the relative position relationship between the injection needle and the blood sample chip in the microfluidic chip reagent injector of the present invention;
wherein: 1. the device comprises a chip turntable, 2, a blood sample chip, 3, a liquid injection unit, 301, a liquid injection needle support, 302, a liquid injection needle, 303, a peristaltic pump, 304, a liquid injection pipe, 4, a height adjusting unit, 401, a lead screw module, 402, a driving motor, 5, a display control unit, 501, a peristaltic pump controller, 502, a programmable controller, 503, a red and green indicator light, 504, a motor controller, 6, a box body shell, 7 and a power supply.
Detailed Description
The following describes embodiments of the present invention with reference to the drawings.
Referring to fig. 1-3, the microfluidic chip reagent injector of the present invention comprises:
the blood sample chip testing device comprises a chip rotary table 1, a blood sample chip 2 is placed on the chip rotary table 1, the chip rotary table 1 drives the blood sample chip 2 to rotate, and a plurality of reaction cavities are uniformly distributed on the circumference of the blood sample chip 2; a rotary motor is arranged in the chip rotary table 1, the table top of the rotary table is driven to rotate through the rotary motor, the blood sample chip 2 is further driven to rotate, and different reaction cavities are adjusted to be opposite to the liquid injection needle 302 of the liquid injection unit 3;
the liquid injection unit 3 comprises a liquid injection needle support 301, a plurality of liquid injection needles 302 which are uniformly distributed on the liquid injection needle support 301 and penetrate through the upper surface and the lower surface, and a liquid conveying unit for conveying liquid to the liquid injection needles 302 in a quantitative manner;
the height adjusting unit 4 drives the liquid injection needle bracket 301 of the liquid injection unit 3 to move up and down, and adjusts the relative distance between the liquid outlet end of the liquid injection needle 302 and the reaction cavity of the blood sample chip 2;
the display control unit 5 is used for controlling the chip turntable 1, the liquid injection unit 3 and the height adjusting unit 4 to act;
and the power supply 7 is used for supplying power to the chip turntable 1, the height adjusting unit 4 and the display control unit 5 by the power supply 7.
The liquid conveying unit comprises a plurality of groups, each group comprises a peristaltic pump 303 and a liquid injection pipe 304, one end of the liquid injection pipe 304 is communicated with a liquid storage bottle for storing liquid, and the other end of the liquid injection pipe passes through the pump head of the peristaltic pump 303 and is connected with the liquid inlet end of the corresponding liquid injection needle 302. The multiple groups of the conveying units correspond to the multiple liquid injection needles 302 one by one. The drawings show only one pour spout 304 for illustration.
The height adjusting unit 4 comprises a lead screw module 401 and a driving motor 402 connected to one end of a lead screw of the lead screw module 401, a nut of the lead screw module 401 and the liquid injection needle support 301 of the liquid injection unit 3 are fixedly connected, and the motor drives the nut and the liquid injection needle support 301 to move up and down.
The display control unit 5 comprises a peristaltic pump controller 501, a programmable controller 502, a motor controller 504 and a traffic light 503; the peristaltic pump controller 501 controls the peristaltic pump 303 to act, and the motor controller 504 controls the height adjusting unit 4 and the chip turntable 1 to act; the red and green indicator light 503 is controlled to be bright or dark by the programmable controller 502.
The filling instrument further comprises a box body shell 6, and the chip rotary table 1, the liquid injection unit 3, the height adjusting unit 4 and the display control unit 5 are all located in the box body shell 6; the chip turntable 1 is arranged on the upper end face of the bottom plate of the box body shell 6; the driving motor 402 of the height adjusting unit 4 is fixed on the box body shell 6, and drives the nut to rotate relative to the lead screw and to do linear motion along the guide rod.
The number of the reaction chambers of the blood sample chip 2 is integral multiple of the number of the injection needles 302 of the injection unit 3. In this embodiment, the number of the reaction chambers of the blood sample chip 2 is 24, and the number of the injection needles 302 is 6. The six liquid injection needles 302 are distributed in an arc and correspond to the reaction cavities on the corresponding blood sample chips 2 one by one.
The utility model discloses a micro-fluidic chip reagent filling appearance working process does:
1) The blood sample chip 2 is placed on the chip rotary table 1, and the blood sample chip 2 is placed randomly. The power is turned on, the programmable controller 502 is selected to automatically execute, and at the moment, a red light in the red-green indicator light 503 is turned on to indicate that liquid injection is not started yet. The chip is driven by the rotating motor to rotate to the initial position, and at the moment, six reaction chambers in any group on the chip correspond to six channels of the liquid injection needle 302 one by one.
2) Pressing a button on the lower side of the chip turntable 1, starting the instrument to work, and lighting a green lamp at the moment to indicate that liquid injection is started;
3) After a group of liquid injection of the blood sample chip 2 is completed, the height adjusting unit 4 and the chip turntable 1 are matched to adjust the next group of reaction chambers to be in one-to-one correspondence with the liquid injection needles 302, liquid injection of one blood sample chip 2 is completed until all reaction chambers of the blood sample chip 2 are injected with liquid, the red light is turned on, the next chip is replaced, and the steps are repeated.
The action process of the whole equipment, the liquid injection time and the liquid injection amount are automatically controlled, the liquid injection time is controlled by a programmable controller 502, and the liquid injection amount is controlled by a peristaltic pump controller 501. The liquid injection needle support 301 is driven by the lead screw to move up and down, so that the liquid injection needle 302 is prevented from colliding with the chip in the liquid injection process.
Claims (6)
1. Micro-fluidic chip reagent filling appearance, its characterized in that includes:
the blood sample chip testing device comprises a chip rotary table (1), wherein a blood sample chip (2) is placed on the chip rotary table (1), the chip rotary table (1) drives the blood sample chip (2) to rotate, and a plurality of reaction cavities are uniformly distributed on the circumference of the blood sample chip (2);
the liquid injection unit (3) comprises a liquid injection needle support (301), a plurality of liquid injection needles (302) which are uniformly distributed on the liquid injection needle support (301) and penetrate through the upper surface and the lower surface, and a liquid conveying unit for conveying liquid to the liquid injection needles (302) quantitatively;
the height adjusting unit (4) drives the liquid injection needle bracket (301) of the liquid injection unit (3) to move up and down, and the relative distance between the liquid outlet end of the liquid injection needle (302) and the reaction cavity of the blood sample chip (2) is adjusted;
the display control unit (5), the display control unit (5) controls the chip turntable (1), the liquid injection unit (3) and the height adjusting unit (4) to act,
and the power supply (7), the power supply (7) supplies power to the chip turntable (1), the height adjusting unit (4) and the display control unit (5).
2. The microfluidic chip reagent filling instrument according to claim 1, wherein the liquid delivery unit comprises a plurality of sets, each set comprises a peristaltic pump (303) and a liquid injection tube (304), one end of the liquid injection tube (304) is communicated with a liquid storage bottle for storing liquid, and the other end of the liquid injection tube passes through a pump head of the peristaltic pump (303) and is connected with a liquid inlet end of a corresponding liquid injection needle (302).
3. The microfluidic chip reagent filling instrument according to claim 1, wherein the height adjusting unit (4) comprises a lead screw module (401) and a driving motor (402) connected to one end of a lead screw of the lead screw module (401), a nut of the lead screw module (401) is fixedly connected with the liquid filling needle support (301) of the liquid filling unit (3), and the motor drives the nut and the liquid filling needle support (301) to move up and down.
4. The microfluidic chip reagent filling instrument according to claim 1, wherein the display control unit (5) comprises a peristaltic pump controller (501), a programmable controller (502), a motor controller (504) and a red-green indicator light (503); the peristaltic pump controller (501) controls the peristaltic pump (303) to act, and the motor controller (504) controls the height adjusting unit (4) and the chip rotary table (1) to act; the red and green indicator light (503) is controlled to be bright or dark through the programmable controller (502).
5. The microfluidic chip reagent filling instrument according to any one of claims 1 to 4, further comprising a box body housing (6), wherein the chip turntable (1), the liquid filling unit (3), the height adjusting unit (4) and the display control unit (5) are all located in the box body housing (6); the chip turntable (1) is arranged on the upper end face of the bottom plate of the box body shell (6); and a driving motor (402) of the height adjusting unit (4) is fixed on the box body shell (6) and drives the screw nut to rotate relative to the screw rod and to do linear motion along the guide rod.
6. The microfluidic chip reagent filling instrument according to any one of claims 1 to 4, wherein the number of reaction chambers of the blood sample chip (2) is an integral multiple of the number of injection needles (302) of the injection unit (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223034480.2U CN218872250U (en) | 2022-11-15 | 2022-11-15 | Micro-fluidic chip reagent filling instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223034480.2U CN218872250U (en) | 2022-11-15 | 2022-11-15 | Micro-fluidic chip reagent filling instrument |
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| Publication Number | Publication Date |
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| CN218872250U true CN218872250U (en) | 2023-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202223034480.2U Active CN218872250U (en) | 2022-11-15 | 2022-11-15 | Micro-fluidic chip reagent filling instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116174073A (en) * | 2023-05-04 | 2023-05-30 | 合肥机数量子科技有限公司 | Automatic high-precision liquid sample injection system and method |
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2022
- 2022-11-15 CN CN202223034480.2U patent/CN218872250U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116174073A (en) * | 2023-05-04 | 2023-05-30 | 合肥机数量子科技有限公司 | Automatic high-precision liquid sample injection system and method |
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