CN201686700U - Tissue cell culture device - Google Patents
Tissue cell culture device Download PDFInfo
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- CN201686700U CN201686700U CN 201020146372 CN201020146372U CN201686700U CN 201686700 U CN201686700 U CN 201686700U CN 201020146372 CN201020146372 CN 201020146372 CN 201020146372 U CN201020146372 U CN 201020146372U CN 201686700 U CN201686700 U CN 201686700U
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- acid solution
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- controlling system
- alkali lye
- control valve
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The utility model discloses a tissue cell culture device, which comprises an incubator provided with a control system and a display screen. A pH sensor, a temperature sensor, a fluid pressure sensor and an oxygen partial pressure sensor are arranged in the incubator, signal output ends of the sensors are respectively connected with a signal receiving end of the control system, and a signal output end of the control system is connected with a signal input end of the display screen. When in use, culture solution and culture tissue are filled in a culture vessel, measuring ends of the sensors are respectively inserted into the culture solution to monitor temperature, oxygen partial pressure, fluid pressure and pH value of the culture solution, and monitoring results for each time are displayed on the display screen through the control system. When in full culture process, constant temperature and culture condition monitoring can be realized by the control system, operators can acquire parameters of the culture solution without opening or closing a door of the incubator frequently, and the culture environment in the incubator can keep stable simultaneously, thereby reducing pollution risk, increasing repeatability of operation and achieving more reliable experimental results.
Description
Technical field
The utility model relates to a kind of medical treatment device, particularly a kind of device that is used to carry out the histocyte cultivation.
Background technology
Tissue culture is that the individuality from multicellular organism sterilely takes out tissue block, cell mass, make it the technology that lives under suitable condition, now not only be widely used in breeding, the differentiation of cell, the research of cancer, but also utilize tissue culture cells to carry out the research of effect of drugs, also constantly be used in the manufacturing vaccine.Tissue culture has dual mode, and the one, cell cultures, the 2nd, tissue block is cultivated.
Tissue block that will be bigger, organ piece remain on and cultivate under the state of three-dimensional structure is one of approach of more often adopting of tissue culture; According to the needed cell count of research, or select the material of different shape, all size for the ease of optical observation during cultivation, substratum mainly uses nutrient solution.
During tissue culture, need to keep stable envrionment conditions, as the oxygen partial pressure in culture-liquid temp, potential of hydrogen and the nutrient solution etc.The tissue culture procedures of prior art generally is that the cultivation vessel are placed thermostat container, then regularly or as required, adopts temperature measuring equipment, pH measurement device etc. to measure, and then obtains required conditional parameter; Open the door of thermostat container during measurement, again door is closed after measurement finishes, operate very inconvenient; What is more important, the continuous switching of thermostat container hermatic door has greatly influenced isoperibol, brings the risk of pollution simultaneously.
Therefore, be necessary to overcome described defective, a kind of histocyte culture apparatus is provided, integrate constant temperature and culture environment monitoring function, can fully keep the stable of culture environment in the tissue culture whole process, make operation repeatability higher, experimental result is more reliable.
The utility model content
In view of this, the purpose of this utility model provides a kind of histocyte culture apparatus, comprise the thermostat container that Controlling System and display screen are set, be provided with pH transmitter, temperature sensor, fluid pressure sensor and oxygen partial pressure transmitter in the constant temperature space of thermostat container, Controlling System is provided with signal input part, signal output part and instruction output end;
The signal output part of the signal output part of pH transmitter, the signal output part of temperature sensor, fluid pressure sensor links to each other with the signal input part of Controlling System respectively with the signal output part of oxygen partial pressure transmitter, and the signal output part of Controlling System links to each other with the signal input part of display screen.
Further, comprise also being used to make nutrient solution round-robin recycle pump that recycle pump is arranged in the constant temperature space of thermostat container, recycle pump and thermostat container common source;
Further, also comprise the flow rate of liquid transmitter and the flow motor-operated control valve that are arranged in the recycle pump liquid circulation runner, the signal output part of flow rate of liquid transmitter links to each other with the signal input part of Controlling System, and the instruction output end of Controlling System links to each other with the command reception end of flow motor-operated control valve; The power source circuit of recycle pump is in parallel with the power source circuit of thermostat container;
Further, also comprise the alkali lye tank and the acid solution tank that all are arranged in the described constant temperature space, the alkali lye tank is provided with alkali liquor inlet and alkali lye outlet, the acid solution tank is provided with acid solution inlet and acid solution outlet, described alkali lye outlet is provided with the flux of alkaline liquor motor-operated control valve, described acid solution outlet is provided with acid solution flow motor-operated control valve, and the instruction output end of Controlling System links to each other with the command reception end of flux of alkaline liquor motor-operated control valve and the command reception end of acid solution flow motor-operated control valve respectively;
Further, also comprise miniature oxygen increasing pump, the power source circuit of miniature oxygen increasing pump is in parallel with the power source circuit of thermostat container;
Described miniature oxygen increasing pump is provided with inlet mouth and air outlet, and the air outlet is provided with the gas flow motor-operated control valve, and the instruction output end of Controlling System links to each other with the command reception end of gas flow motor-operated control valve;
Further, also comprise the culture vessel that is arranged in the described constant temperature space, culture vessel is provided with cultured tissue inlet, nutrient solution inlet, nutrient solution outlet, alkali lye import, acid solution import and oxygen intake, the liquid outlet of recycle pump is communicated with described nutrient solution inlet, the fluid inlet of recycle pump is communicated with described nutrient solution outlet, the alkali lye outlet of alkali lye tank is communicated with described alkali lye import, the acid solution outlet of acid solution tank is communicated with described acid solution import, and the air outlet of miniature oxygen increasing pump is communicated with described oxygen intake;
Further, described nutrient solution inlet, alkali lye import and acid solution import all are arranged at the container top, and nutrient solution outlet and oxygen intake all are arranged at the container below, and culture vessel is an open container, and the uncovered of container is that cultured tissue enters the mouth.
The beneficial effects of the utility model: histocyte culture apparatus of the present utility model comprises the thermostat container that Controlling System and display screen are set; Be provided with pH transmitter, temperature sensor, fluid pressure sensor and oxygen partial pressure transmitter in the constant temperature space of thermostat container, the signal output part of the signal output part of temperature sensor, the signal output part of fluid pressure sensor, pH transmitter links to each other with the signal receiving end of Controlling System respectively with the signal output part of oxygen partial pressure transmitter, and the signal output part of Controlling System links to each other with the signal input part of display screen; During use, in cultivating vessel, pack into nutrient solution and cultured tissue, measuring junction with pH transmitter, temperature sensor, oxygen partial pressure transmitter and fluid pressure sensor probes in the nutrient solution respectively again, each transmitter is monitored temperature, oxygen partial pressure, hydrodynamicpressure and the pH of nutrient solution, and each monitoring result constantly shows on display screen by Controlling System; In the full culturing process, constant temperature and culture condition monitoring realize automatically by Controlling System, and the door that the operator need not to open and close repeatedly thermostat container also can obtain each parameter of nutrient solution, keep the stable of the interior culture environment of case simultaneously, reduce Pollution risk, make operation repeatability higher, experimental result is more reliable.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is further described.
Fig. 1 is a frontview of the present utility model;
Fig. 2 is the utility model internal structure synoptic diagram;
Fig. 3 is the utility model schematic diagram.
Embodiment
Fig. 1 is a frontview of the present utility model; Fig. 2 is the utility model internal structure synoptic diagram; Fig. 3 is the utility model schematic diagram.
As shown in the figure:
The histocyte culture apparatus of present embodiment, comprise the thermostat container 1 that Controlling System 6 and display screen 7 are set, be provided with pH transmitter 2, temperature sensor 3, fluid pressure sensor 4 and oxygen partial pressure transmitter 5 in the constant temperature space of thermostat container, Controlling System 6 is provided with signal input part, signal output part and instruction output end; The signal output part of the signal output part of the signal output part of the signal output part of pH transmitter 2, temperature sensor 3, fluid pressure sensor 4 and oxygen partial pressure transmitter 4 links to each other with the signal input part of Controlling System 6 respectively, and the signal output part of Controlling System 6 links to each other with the signal input part of display screen; During use, in cultivating vessel, pack into nutrient solution and cultured tissue inserted in the vessel, measuring junction with pH transmitter, temperature sensor, oxygen partial pressure transmitter and fluid pressure sensor probes in the nutrient solution respectively again, each transmitter is monitored temperature, oxygen partial pressure, hydrodynamicpressure and the pH of nutrient solution, and each monitoring result constantly shows on display screen by Controlling System; In the full culturing process, constant temperature and culture condition monitoring realize automatically by Controlling System, and the door that the operator need not to open and close repeatedly thermostat container also can obtain each parameter of nutrient solution, keep the stable of the interior culture environment of case simultaneously, reduce Pollution risk, make operation repeatability higher, experimental result is more reliable.
Further improvement as previous embodiment, the utility model also comprises and is used to make nutrient solution round-robin recycle pump 8, recycle pump can be a peristaltic pump, recycle pump is arranged in the constant temperature space of thermostat container, during use, the fluid inlet of recycle pump and liquid outlet insert in the nutrient solution of cultivating vessel, recycle pump constantly circulates nutrient solution, when being used for tissue block cultivated, can strengthen the circulation of nutrient solution in tissue block, ensure culture effect, recycle pump places in the thermostat container, makes nutrient solution keep homo(io)thermism under cycling condition; Recycle pump and thermostat container common source, apparatus structure is compacter, more convenient operation, recycle pump can also can be parallel connection for connecting with the mode of thermostat container power sharing; During series connection, thermostat container power switch and recycle pump power switch are opened simultaneously, the two can start, and thermostat container power switch or recycle pump power switch are closed, and the two promptly quits work simultaneously; When in parallel, the two is subjected to the control of power switch separately and works alone, and is interference-free.
Further improvement as previous embodiment, the utility model also comprises flow rate of liquid transmitter 10 and the flow motor-operated control valve 9 that is arranged in the recycle pump liquid circulation runner, the signal output part of flow rate of liquid transmitter 10 links to each other with the signal input part of Controlling System 6, and the instruction output end of Controlling System 6 links to each other with the command reception end of flow motor-operated control valve 9; During use, flow rate of liquid sensor detecting fluid flow rate signal also is sent to Controlling System with signal, and Controlling System carries out showing by display screen after the conversion of signals; The operator passes through the parameter of the data grasp nutrient solution flow rate of display screen, and when needing to adjust the flow size, input order in Controlling System instructs to be received and carry out by the flow motor-operated control valve, thus the adjustment of nutrient solution flow in the realization liquid circulation runner; The power source circuit of recycle pump is in parallel with the power source circuit of thermostat container, realizes the independent control of recycle pump and thermostat container, and suitability is better.
Further improvement as the foregoing description, the utility model also comprises alkali lye tank 15 and the acid solution tank 16 that all is arranged in the described constant temperature space, alkali lye tank 15 is provided with alkali liquor inlet and alkali lye outlet 151, acid solution tank 16 is provided with acid solution inlet and acid solution outlet 161, alkali lye outlet 151 is provided with flux of alkaline liquor motor-operated control valve 152, acid solution outlet 161 is provided with acid solution flow motor-operated control valve 162, and the instruction output end of Controlling System 6 links to each other with the command reception end of flux of alkaline liquor motor-operated control valve 152 and the command reception end of acid solution flow motor-operated control valve 162 respectively; During use, inject alkaline solution to the alkali lye tank, inject acid solution to the acid solution tank, by the potential of hydrogen of pH sensor detecting nutrient solution and with the detection signal input control system, when nutrient solution acidity is too high, the Controlling System instruction output end is to flux of alkaline liquor motor-operated control valve 152 output orders, flux of alkaline liquor motor-operated control valve release portion alkali lye enters in the nutrient solution, when nutrient solution basicity is too high, the Controlling System instruction output end is to acid solution flow motor-operated control valve 162 output orders, acid solution flow motor-operated control valve release portion acid solution enters in the nutrient solution, thereby realizes the automatic adjustment of nutrient solution potential of hydrogen, and is easy to use and ensure isoperibol in the constant temperature space.In the present embodiment, alkali lye tank and acid solution tank are the open container (not shown), and the uncovered of alkali lye tank is alkali liquor inlet, and the uncovered of acid solution tank is that acid solution enters the mouth; Acid solution tank and alkali lye tank can be made into one, make, install and use more convenient.
As the further improvement of the foregoing description, the utility model comprises also and also comprises miniature oxygen increasing pump 17 that the power source circuit of miniature oxygen increasing pump is in parallel with the power source circuit of thermostat container, is convenient to it and independently controls; Miniature oxygen increasing pump is provided with inlet mouth (not shown) and air outlet 171, and air outlet 171 is provided with gas flow motor-operated control valve 172, and the instruction output end of Controlling System 6 links to each other with the command reception end of gas flow motor-operated control valve 172; During operation, by the oxygen partial pressure transmitter at any time or the oxygen partial pressure in the periodic monitor nutrient solution and with the monitor signal input control system, when oxygen partial pressure is crossed when low, Controlling System is to the gas flow motor-operated control valve output order on the miniature oxygen increasing pump, the gas flow motor-operated control valve is opened, miniature oxygen increasing pump can be in nutrient solution oxygenation, after oxygen partial pressure reaches required standard, Controlling System is output order once again, gas flow control valve is closed, thereby the automatic adjustment of oxygen partial pressure in the realization nutrient solution ensures the reliable and stable of culture environment.
Further improvement as the foregoing description, the utility model also comprises the culture vessel 11 that is arranged in the described constant temperature space, culture vessel is provided with cultured tissue inlet 12, nutrient solution inlet 13, nutrient solution outlet 14, alkali lye import 18, acid solution import 19 and oxygen intake 20, the liquid outlet 81 of recycle pump is communicated with described nutrient solution inlet 13, the fluid inlet 82 of recycle pump is communicated with described nutrient solution outlet 14, the alkali lye of alkali lye tank outlet 151 is communicated with described alkali lye import 18, the acid solution of acid solution tank outlet 161 is communicated with described acid solution import 19, and the air outlet 171 of miniature oxygen increasing pump is communicated with described oxygen intake 20; This structure makes the device globality better, and the connection of each parts is more convenient in the device.
Further improvement as the foregoing description, in the utility model, described nutrient solution inlet 13, alkali lye import 18 and acid solution import 19 all are arranged at the container top, nutrient solution outlet 14 and oxygen intake 20 all are arranged at the container below, be convenient to the thorough mixing of solution, culture vessel is an open container, and the uncovered of container is that cultured tissue enters the mouth, and inserting of cultured tissue is more convenient.In the present embodiment, alkali lye import 18 and acid solution import 19 all are arranged on the culture vessel and export 14 relative sides with nutrient solution, after being convenient to alkaline solution or acid solution and adding, can mix more fully, ensure that further experimental result accurately and reliably.
Explanation is at last, above embodiment is only unrestricted in order to the explanation the technical solution of the utility model, although the utility model is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the technical solution of the utility model, and not breaking away from the aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of the claim scope of the present utility model.
Claims (6)
1. histocyte culture apparatus, it is characterized in that: comprise the thermostat container (1) that Controlling System (6) and display screen (7) are set, be provided with pH transmitter (2), temperature sensor (3), fluid pressure sensor (4) and oxygen partial pressure transmitter (5) in the constant temperature space of thermostat container, Controlling System (6) is provided with signal input part, signal output part and instruction output end;
The signal output part of the signal output part of the signal output part of the signal output part of pH transmitter (2), temperature sensor (3), fluid pressure sensor (4) and oxygen partial pressure transmitter (4) links to each other with the signal input part of Controlling System (6) respectively, and the signal output part of Controlling System (7) links to each other with the signal input part of display screen.
2. histocyte culture apparatus according to claim 1 is characterized in that: comprise also being used to make nutrient solution round-robin recycle pump (8) that recycle pump (8) is arranged in the constant temperature space of thermostat container, recycle pump and thermostat container common source.
3. histocyte culture apparatus according to claim 2, it is characterized in that: also comprise the flow rate of liquid transmitter (10) and the flow motor-operated control valve (9) that are arranged in recycle pump (8) the liquid circulation runner, the signal output part of flow rate of liquid transmitter (10) links to each other with the signal input part of Controlling System (6), and the instruction output end of Controlling System (6) links to each other with the command reception end of flow motor-operated control valve (9); The power source circuit of recycle pump is in parallel with the power source circuit of thermostat container.
4. histocyte culture apparatus according to claim 3, it is characterized in that: also comprise the alkali lye tank (15) and the acid solution tank (16) that all are arranged in the described constant temperature space, alkali lye tank (15) is provided with alkali liquor inlet and alkali lye outlet (151), acid solution tank (16) is provided with acid solution inlet and acid solution outlet (161), described alkali lye outlet (151) is provided with flux of alkaline liquor motor-operated control valve (152), described acid solution outlet (161) is provided with acid solution flow motor-operated control valve (162), and the instruction output end of Controlling System (6) links to each other with the command reception end of flux of alkaline liquor motor-operated control valve (152) and the command reception end of acid solution flow motor-operated control valve (162) respectively.
5. histocyte culture apparatus according to claim 4 is characterized in that: also comprise miniature oxygen increasing pump (17), the power source circuit of miniature oxygen increasing pump is in parallel with the power source circuit of thermostat container; Described miniature oxygen increasing pump is provided with inlet mouth and air outlet, and the air outlet is provided with gas flow motor-operated control valve (172), and the instruction output end of Controlling System (6) links to each other with the command reception end of gas flow motor-operated control valve (172).
6. histocyte culture apparatus according to claim 5, it is characterized in that: also comprise the culture vessel (11) that is arranged in the described constant temperature space, culture vessel is provided with cultured tissue inlet (12), nutrient solution inlet (13), nutrient solution outlet (14), alkali lye import (18), acid solution import (19) and oxygen intake (20), the liquid outlet of recycle pump and described nutrient solution enter the mouth (13) be communicated with, the fluid inlet of recycle pump exports (14) with described nutrient solution and is communicated with, the alkali lye outlet (151) of alkali lye tank is communicated with described alkali lye import (18), the acid solution outlet (161) of acid solution tank is communicated with described acid solution import (19), and the air outlet of miniature oxygen increasing pump (171) are communicated with described oxygen intake (20).
7. histocyte culture apparatus according to claim 6, it is characterized in that: described nutrient solution inlet (13), alkali lye import (18) and acid solution import (19) all are arranged at the container top, nutrient solution outlet (14) and oxygen intake (20) all are arranged at the container below, culture vessel is an open container, and the uncovered of container is that cultured tissue enters the mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020146372 CN201686700U (en) | 2010-03-31 | 2010-03-31 | Tissue cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020146372 CN201686700U (en) | 2010-03-31 | 2010-03-31 | Tissue cell culture device |
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| CN201686700U true CN201686700U (en) | 2010-12-29 |
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| CN 201020146372 Expired - Fee Related CN201686700U (en) | 2010-03-31 | 2010-03-31 | Tissue cell culture device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232937A (en) * | 2013-04-18 | 2013-08-07 | 刘傥 | Cell pressure test system |
| CN104475180A (en) * | 2014-12-15 | 2015-04-01 | 成都红柿子科技有限公司 | Automatic temperature-control portable culture solution incubator |
| CN104498358A (en) * | 2014-12-18 | 2015-04-08 | 宁波新芝生物科技股份有限公司 | Integrated cell culture machine |
| CN108641958A (en) * | 2018-08-08 | 2018-10-12 | 南京师范大学 | A kind of biology 3 D-printing environment real time monitoring and active maintenance device |
-
2010
- 2010-03-31 CN CN 201020146372 patent/CN201686700U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232937A (en) * | 2013-04-18 | 2013-08-07 | 刘傥 | Cell pressure test system |
| CN104475180A (en) * | 2014-12-15 | 2015-04-01 | 成都红柿子科技有限公司 | Automatic temperature-control portable culture solution incubator |
| CN104498358A (en) * | 2014-12-18 | 2015-04-08 | 宁波新芝生物科技股份有限公司 | Integrated cell culture machine |
| CN104498358B (en) * | 2014-12-18 | 2016-01-27 | 宁波新芝生物科技股份有限公司 | A kind of cell cultures all-in-one |
| CN108641958A (en) * | 2018-08-08 | 2018-10-12 | 南京师范大学 | A kind of biology 3 D-printing environment real time monitoring and active maintenance device |
| CN108641958B (en) * | 2018-08-08 | 2024-02-23 | 南京师范大学 | Biological three-dimensional printing environment real-time monitoring and activity maintaining device |
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Granted publication date: 20101229 Termination date: 20110331 |