CN206940913U - A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems - Google Patents
A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems Download PDFInfo
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- CN206940913U CN206940913U CN201720829299.0U CN201720829299U CN206940913U CN 206940913 U CN206940913 U CN 206940913U CN 201720829299 U CN201720829299 U CN 201720829299U CN 206940913 U CN206940913 U CN 206940913U
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Abstract
A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems, belong to biological culture fermentation arts.It is characterized in that：It is provided with cleaning stock bottle（1）, clean stock bottle（1）It is connected to cleaning control module（3）Entrance, cleaning control module（3）Outlet be connected to the entrance of feeding system, the outlet of feeding system is connected with driver element, cleans stock bottle（1）In cleaning fluid feeding system is flowed through under the driving of feeding system driver element and is ultimately drained into liquid waste collector after being discharged by driver element（13）.In the feeding pipeline cleaning and sterilizing system that this is used for high-flux cell culture systems, directly it can be cleaned, be disinfected for the feeding pipeline system of high-flux cell culture systems, avoid the various drawbacks for needing to be cleaned and sterilized after dismounting in the prior art, automaticity is high, the risk of cross pollution when avoiding manual operation, while there is more preferable cleaning and sterilizing effect.
Description
Technical field
A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems, belong to biological culture fermentation neck
Domain.
Background technology
High-flux cell culture systems are to be directed to various kinds of cell（Microorganism, lactation, insect and plant cell etc.）, in milliliter
In liter volume of culture level, parallel, high-throughout development is multiple, and simulation industrializes cell cultivation process completely, opens
Exhibition is evaluated as the full-automatic cell culture experiment of main purpose with DoE cell clones.
Need to clean the pipeline of feeding system after experiment each time is completed, because whole experiment process needs
Carried out in the environment of integral asepsis, therefore the cleaning to testing pipeline also requires higher.In the prior art, conventional cleaning
Method is individually to be cleaned, sterilized after supply lines are pulled down after the completion of experiment, finally carries out the assembling of pipeline, carries out
Experiment next time.Because cleaning is required for carrying out the dismounting of pipeline every time, therefore whole process is relatively complicated, while manually exist
Bacterium mixed probability is equally existed when being installed, causes cross pollution, therefore the whole effect cleaned and sterilized is difficult to
Ensure.
The content of the invention
The technical problems to be solved in the utility model is：Overcome the deficiencies in the prior art, there is provided one kind can be directly height
The feeding pipeline cleaning and sterilizing system of throughput cell culture systems is cleaned, and avoid needs dismounting to carry out clearly in the prior art
The various drawbacks washed, automaticity is high, the risk of cross pollution when avoiding manual operation, while cleaning and Disinfection Effect are more
The good feeding pipeline cleaning and sterilizing system for high-flux cell culture systems.
Technical scheme is used by the utility model solves its technical problem：This is used for high-flux cell culture systems
Feeding pipeline cleaning and sterilizing system, include the feeding system of high-flux cell culture systems, it is characterised in that：It is provided with multiple clear
Stock bottle is washed, cleaning stock bottle is connected to the porch of cleaning control module, and the outlet of cleaning control module is connected to the confession
The entrance of material system, the outlet of feeding system are connected with driver element, drive of the cleaning fluid cleaned in stock bottle in driver element
Feeding system is flowed through under dynamic and is discharged by driver element.
Preferably, described cleaning control module includes shell and the control circuit being arranged in shell, cleaning control
The entrance and cleaning stock bottle of module correspond, and divide on connecting line of the cleaning control module inlet with cleaning stock bottle
A magnetic valve controlled by control circuit is not provided with.
Preferably, the liquid separatnig valve of a multi-port input single port output, liquid separation are provided with described cleaning control module
The input port of valve corresponds with cleaning stock bottle, and its delivery outlet is the delivery outlet of cleaning control module.
Preferably, described cleaning stock bottle is provided with three, is contained with the sodium hydroxide raw material as cleaning fluid respectively
Bottle, the alcohol raw material bottle that the concentration as thimerosal is 75% and the pure water stock bottle as flushing liquor.
Preferably, in described feeding system, multiple feed bottles is provided with and are fed correspondingly with feed bottle
Pipeline, one end of supply lines and cleaning control module and corresponding feed bottle connect, the other end respectively with corresponding driving
Unit connects.
Preferably, described feed bottle includes：Acid solution feed bottle, alkaline solution feed bottle, defoaming solution feed bottle
And feed solutions feed bottle, described supply lines are to be supplied with acid solution feed bottle, alkaline solution feed bottle, defoaming solution
Acid solution pipeline, alkaline solution pipeline, defoaming solution pipeline, feed supplement are molten corresponding to expecting bottle and feed solutions feed bottle respectively
Liquid pipeline.
Preferably, described driver element is located at the one-to-one peristaltic pump of feed bottle.
Preferably, the outlet of described peristaltic pump accesses liquid waste collector by pipeline.
Preferably, described control circuit includes controller, timing module and button.
Compared with prior art, beneficial effect possessed by the utility model is：
In the feeding pipeline cleaning and sterilizing system that this is used for high-flux cell culture systems, needing to enter feeding system
During row cleaning, cleaning control module is accessed in feeding system, the cleaning fluid cleaned in stock bottle is under the driving of driver element
Flow through feeding system and discharged by driver element, avoid needs to be required to enter feeding system during cleaning in the prior art every time
The drawbacks of row disassembly, cleaning, sterilization, automaticity is high, the risk of cross pollution when avoiding manual operation, and operation is more simple
It is clean, due to the effect without being assembled again after sterilization, therefore with preferably cleaning, sterilization.
Brief description of the drawings
Fig. 1 is the feeding pipeline cleaning and sterilizing system pipeline connection diagram for high-flux cell culture systems.
Wherein：1st, stock bottle 2, magnetic valve 3, cleaning control module 4, acid solution feed bottle 5, acid solution are cleaned
Pipeline 6, alkaline solution feed bottle 7, alkaline solution pipeline 8, defoaming solution feed bottle 9, defoaming solution pipeline 10, feed supplement
Solution feed bottle 11, feed solutions pipeline 12, peristaltic pump 13, liquid waste collector.
Embodiment
Fig. 1 is most preferred embodiment of the present utility model, and 1 pair of the utility model is described further below in conjunction with the accompanying drawings.
As shown in figure 1, a kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems, including cleaning are former
Expect bottle 1, multiple magnetic valves 2 and cleaning control module 3, cleaning stock bottle 1 includes three, respectively sodium hydroxide stock bottle,
Alcohol raw material bottle and pure water stock bottle, the alcoholic solution that concentration is 75%, sodium hydroxide are contained with wherein alcohol raw material bottle
Solution, 75% alcoholic solution and pure water are respectively as cleaning fluid, thimerosal, flushing liquor.Divide from three cleaning stock bottles 1
Not Yin Chu pipeline be connected to the different porch of cleaning control module 3, in cleaning control module 3, each porch is set respectively
There is a magnetic valve 2, cleaning stock bottle 1 accesses cleaning control module 3 by corresponding magnetic valve 2 respectively.Cleaning control mould
It is the feeder sleeve in feeding system in the feeding pipeline cleaning and sterilizing system of the outlet access high-flux cell culture systems of block 3
Road is cleaned, and in the outlet access liquid waste collector 13 of feeding system, the waste liquid after cleaning is collected.
Cleaning control module 3 includes shell and the control circuit being arranged in shell, and control circuit includes controller, meter
When module and button, controller the on off operating mode of above-mentioned magnetic valve 2 is controlled respectively.It is additionally provided with shell point
Liquid valve, liquid separatnig valve are provided with three entrances and one outlet, can realized by four-way pipe, wherein three entrances correspond to access respectively
Three above-mentioned cleaning stock bottles 1, are exported as the outlet of cleaning control module 3.
The outlet of cleaning control module 3 accesses the feeding pipeline cleaning and sterilizing system of high-flux cell culture systems by pipeline
In system, in the feeding pipeline cleaning and sterilizing system of high-flux cell culture systems, including four feeding containers：Acid solution supplies
Bottle 4, alkaline solution feed bottle 6, defoaming solution feed bottle 8 and feed solutions feed bottle 10 are expected, in the He of acid solution feed bottle 4
Acid solution and alkaline solution are contained with respectively in alkaline solution feed bottle 6, are respectively used to adjust nutrient solution in experimentation
Acid-base value；Solution in defoaming solution feed bottle 8 is used to eliminate caused bubble in experimentation；Feed solutions feed bottle 10 is used
In supplementing necessary nutritive solution into experimentation.Supplied from acid solution feed bottle 4, alkaline solution feed bottle 6, defoaming solution
Acid solution pipeline 5, alkaline solution pipeline 7, defoaming solution pipeline are drawn in material bottle 8, the top of feed solutions feed bottle 10 respectively
9th, feed solutions pipeline 11, acid solution pipeline 5, alkaline solution pipeline 7, defoaming solution pipeline 9, feed solutions pipeline 11 it is another
In the retort of one end insert experiment.Be additionally provided with respectively with acid solution pipeline 5, alkaline solution pipeline 7, defoaming solution pipeline
9th, 11 one-to-one peristaltic pumps 12 of feed solutions pipeline.
Specific work process and operation principle are as follows：
, first will be acid molten when needing to clean the supply lines in high-flux cell culture systems feeding system
Liquid pipeline 5, alkaline solution pipeline 7, defoaming solution pipeline 9, feed solutions pipeline 11 are from corresponding acid solution feed bottle 4, alkali
Property solution feed bottle 6, defoaming solution feed bottle 8, remove in feed solutions feed bottle 10, be then connected to cleaning control mould simultaneously
The outlet of block 3, acid solution pipeline 5, alkaline solution pipeline 7, defoaming solution pipeline 9, feed solutions pipeline 11 the other end from
Removed in retort and be connected respectively to the porch of corresponding peristaltic pump 12, the outlet of peristaltic pump 12 accesses waste liquid receipts simultaneously
In storage 13.
When being cleaned, it is necessary to respectively to acid solution pipeline 5, alkaline solution pipeline 7, defoaming solution pipeline 9 and
Feed solutions pipeline 11 is cleaned, by taking acid solution pipeline 5 as an example：Peristaltic pump 12 corresponding with acid solution pipeline 5 works,
The cleaning solution exported in cleaning control module 3 is introduced into acid solution pipeline 5.Control in cleaning control module 3 simultaneously
The connection of three magnetic valves 2 of circuit Time-sharing control, the company of sodium hydroxide solution, alcoholic solution and pure water circuit is realized respectively
It is logical, and timing is carried out by timing module, after reaching timing time, control circuit controls the switching of magnetic valve 2, therefore hydroxide
Sodium solution, alcoholic solution and pure water flow separately through acid solution pipeline 5 in the presence of peristaltic pump 12, are respectively completed to acidity
Cleaning, sterilization and the flushing of solution line 5, when being cleaned, being sterilized and being rinsed, corresponding solution is sent into by peristaltic pump 12
It is collected in liquid waste collector 13.The cleaning of alkaline solution pipeline 7, defoaming solution pipeline 9 and feed solutions pipeline 11
Cheng Xiangtong, it will not be repeated here.
It is described above, only it is preferred embodiment of the present utility model, is not that other forms are made to the utility model
Limitation, any person skilled in the art is changed or is modified as possibly also with the technology contents of the disclosure above equivalent
The equivalent embodiment of change.But it is every without departing from the content of the technical scheme of the utility model, it is real according to technology of the present utility model
Any simple modification, equivalent variations and the remodeling that confrontation above example is made, still fall within the guarantor of technical solutions of the utility model
Protect scope.
Claims (9)
1. a kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems, including high-flux cell culture systems
Feeding system, it is characterised in that：It is provided with multiple cleaning stock bottles（1）, clean stock bottle（1）It is connected to cleaning control module
（3）Porch, cleaning control module（3）Outlet be connected to the entrance of the feeding system, the outlet and drive of feeding system
Moving cell is connected, and cleans stock bottle（1）In cleaning fluid feeding system is flowed through under the driving of driver element and by driver element
Discharge.
2. the feeding pipeline cleaning and sterilizing system according to claim 1 for high-flux cell culture systems, its feature
It is：Described cleaning control module（3）Including shell and the control circuit being arranged in shell, cleaning control module（3）
Entrance with cleaning stock bottle（1）Correspond, and in cleaning control module（3）Entrance and cleaning stock bottle（1）Connecting tube
A magnetic valve controlled by control circuit is respectively arranged with road（2）.
3. the feeding pipeline cleaning and sterilizing system according to claim 2 for high-flux cell culture systems, its feature
It is：In described cleaning control module（3）In be provided with the liquid separatnig valve of multi-port input single port output, the input of liquid separatnig valve
Port and cleaning stock bottle（1）Correspond, its delivery outlet is cleaning control module（3）Delivery outlet.
4. the feeding pipeline cleaning and sterilizing system for high-flux cell culture systems according to any one of claim 1 ~ 3
System, it is characterised in that：Described cleaning stock bottle（1）Three are provided with, is contained with respectively former as the sodium hydroxide of cleaning fluid
Expect bottle, the alcohol raw material bottle that the concentration as thimerosal is 75% and the pure water stock bottle as flushing liquor.
5. the feeding pipeline cleaning and sterilizing system according to claim 1 for high-flux cell culture systems, its feature
It is：In described feeding system, be provided with multiple feed bottles and with the one-to-one supply lines of feed bottle, feeder sleeve
The one end on road and cleaning control module（3）And corresponding feed bottle connection, the other end connect with corresponding driver element respectively.
6. the feeding pipeline cleaning and sterilizing system according to claim 5 for high-flux cell culture systems, its feature
It is：Described feed bottle includes：Acid solution feed bottle（4）, alkaline solution feed bottle（6）, defoaming solution feed bottle（8）With
And feed solutions feed bottle（10）, described supply lines are and acid solution feed bottle（4）, alkaline solution feed bottle（6）, disappear
Steep solution feed bottle（8）And feed solutions feed bottle（10）Acid solution pipeline corresponding to respectively（5）, alkaline solution pipeline
（7）, defoaming solution pipeline（9）, feed solutions pipeline（11）.
7. being used for the feeding pipeline cleaning and sterilizing system of high-flux cell culture systems according to claim 1 or 5, it is special
Sign is：Described driver element is located at the one-to-one peristaltic pump of feed bottle（12）.
8. the feeding pipeline cleaning and sterilizing system according to claim 7 for high-flux cell culture systems, its feature
It is：Described peristaltic pump（12）Outlet liquid waste collector is accessed by pipeline（13）.
9. the feeding pipeline cleaning and sterilizing system according to claim 2 for high-flux cell culture systems, its feature
It is：Described control circuit includes controller, timing module and button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720829299.0U CN206940913U (en) | 2017-07-10 | 2017-07-10 | A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720829299.0U CN206940913U (en) | 2017-07-10 | 2017-07-10 | A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems |
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| CN206940913U true CN206940913U (en) | 2018-01-30 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109533418A (en) * | 2018-11-22 | 2019-03-29 | 英诺维尔智能科技（苏州）有限公司 | It is a kind of to guarantee the reagent container of sterile replacement in non-clean environment |
| CN110129200A (en) * | 2019-05-15 | 2019-08-16 | 林伟阳 | A kind of 3D stem cell emulation culture device |
-
2017
- 2017-07-10 CN CN201720829299.0U patent/CN206940913U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109533418A (en) * | 2018-11-22 | 2019-03-29 | 英诺维尔智能科技（苏州）有限公司 | It is a kind of to guarantee the reagent container of sterile replacement in non-clean environment |
| CN110129200A (en) * | 2019-05-15 | 2019-08-16 | 林伟阳 | A kind of 3D stem cell emulation culture device |
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