CN211771315U - Sterile ultrasonic humidifying device for air blowing fermentation - Google Patents

Abstract

The utility model relates to an aseptic ultrasonic humidification device for fermentation of blowing, it includes water filling port, storage water tank, water level controller, atomizing case, gas outlet, water level line, ultrasonic atomization module and air inlet. The top of the water storage tank is provided with a water filling port, and the bottom of the water storage tank is connected with the atomization tank; the left side and the right side of the atomization box are respectively provided with an air inlet and an air outlet, and a water level controller and an ultrasonic atomization module are distributed in the atomization box; the ultrasonic atomization module adopts an ultrasonic atomization sheet with the diameter of 20mm, and the atomization amount per hour can reach 300 milliliters. The utility model has simple structure and convenient use, can continuously provide saturated vapor when used for the microorganism fermentation culture by blowing, thereby slowing down the evaporation speed of the fermentation liquor in the fermentation bottle, maintaining the osmotic pressure and acid-base balance of the fermentation liquor, meeting the requirements of different microorganisms on the fermentation culture time and increasing the biomass; compared with the prior ultrasonic humidifying device, the utility model discloses the cost of manufacture is low, and can be connected with air pump, millipore filtration etc. and use.

Description

Sterile ultrasonic humidifying device for air blowing fermentation

Technical Field

The utility model relates to an ultrasonic humidifying device, in particular to an aseptic ultrasonic humidifying device for blowing, fermenting and culturing microorganisms.

Background

The fermentation culture of microorganisms by a blowing method is a mode for carrying out microorganism culture in a laboratory, can increase the biomass of liquid fermentation culture, but in the actual culture process, along with the increase of the culture time, fermentation liquor in a fermentation bottle is gradually reduced due to evaporation, and the growth and the propagation of microorganisms are influenced by the change of osmotic pressure, pH value and nutrient concentration, so that the maintenance of the relative humidity in the fermentation bottle is very important in the microorganism culture process. When the microorganism test is carried out by blowing fermentation, in order to avoid polluting fermentation liquor, saturated steam introduced into the fermentation bottle needs to be ensured to be free of mixed bacteria, but the existing ultrasonic humidifier can only be used independently and cannot be connected with an air pump, a filter membrane and the like despite various types. Therefore, the existing ordinary ultrasonic humidifier cannot be used for such experiments.

SUMMERY OF THE UTILITY MODEL

The utility model aims to increase the relative humidity in the fermentation bottle in the process of blowing, fermenting and culturing microorganisms by utilizing an ultrasonic humidifying device.

The utility model discloses a structure includes: the water-spraying device comprises a water filling port 1, a water storage tank 2, a water level controller 3, an atomization box 4, an air outlet 5, a water level line 6, an ultrasonic atomization module 7 and an air inlet 8.

When using the utility model discloses the time, follow water filling port 1 to storage water tank 2 in water injection, then link to each other air inlet 8 with the air pump through the rubber tube, connect the rubber tube and insert in the fermentation bottle after gas outlet 5 links to each other with microporous membrane. After the power supply is switched on, the air pump blows air into the air inlet 8, the ultrasonic atomization module 7 continuously works, and the generated saturated vapor passes through the microporous filter membrane from the air outlet 5 along with the air flow and then enters the fermentation bottle to maintain the humidity required by the microbial fermentation culture. When the water level line 6 is about 2-4cm higher than the ultrasonic atomization module 7, the working effect of the ultrasonic atomization module 7 is best, when the water level line 6 is lower, the water level controller 3 is triggered, and water in the water storage tank 2 flows into the atomization box 4, so that the normal operation of the ultrasonic atomization module 7 is ensured.

Compared with the prior invention, the utility model has the following advantages:

(1) the humidifying device has simple structure and convenient use;

(2) an ultrasonic atomization module in the ultrasonic humidifying device adopts an ultrasonic atomization sheet with the diameter of 20mm, and the atomization amount per hour can reach 300 milliliters;

(3) the two sides of the ultrasonic humidifying device can be connected with devices such as an air pump, a microporous filter membrane and the like, so that the problem that a common humidifier cannot be used with other experimental instruments is solved;

(4) the water level controller in the ultrasonic humidifying device can automatically control the water capacity in the atomizing box, so that the normal operation of the ultrasonic atomizing module is ensured;

(5) when the ultrasonic humidifier is used for culturing microorganisms through blowing fermentation, saturated water vapor can be continuously provided, so that the evaporation speed of fermentation liquor in a fermentation bottle is slowed down, the osmotic pressure balance of the fermentation liquor is maintained, different requirements of different microorganisms on fermentation time are met, and the biomass is increased;

drawings

FIG. 1 is a schematic view of the overall structure of an ultrasonic humidifier;

fig. 2 is a schematic top view of the inside of an atomization box in the ultrasonic humidification apparatus.

In the figure, a water filling port 1, a water storage tank 2, a water level controller 3, an atomization box 4, an air outlet 5, a water level line 6, an ultrasonic atomization module 7 and an air inlet 8 are arranged

Detailed Description

The following is explained in conjunction with the drawings:

take the case of air-blown fermentation culture of Chinese Potentilla Spinosa.

Injecting water into the water storage tank 2 from the water injection port 1, connecting the air inlet 8 with an air pump through a rubber tube, connecting the air outlet 5 with a microporous filter membrane, connecting the rubber tube, and inserting into a 3L fermentation bottle (the fermentation bottle contains 1.5L of liquid culture medium and is inoculated with Populus peltatus in advance). After the system is powered on, the ultrasonic atomization module 7 continuously works to generate saturated water vapor, the air pump blows air into the air inlet 8 to blow out the saturated water vapor from the air outlet 5, and the saturated water vapor enters the fermentation bottle after passing through the microporous filter membrane to maintain the humidity required by the fermentation culture of the Chinese Potentilla chinensis. When the water level line 6 is higher than the ultrasonic atomization module 7 by about 2-4cm, the working effect of the ultrasonic atomization module 7 is optimal, when the water level line 6 is lower, the water level controller 3 is triggered, and water in the water storage tank 2 flows into the atomization box 4, so that the normal operation of the ultrasonic atomization module 7 is ensured. The use of the utility model discloses blow the fermentation and cultivate chinese ghost pen that looses tail and ordinary blow the fermentation and cultivate contrast, the result is as shown in the following table.

When the utility model is used for air blowing fermentation culture of the Chinese Podophyllum peltatum, the volume of the residual liquid in the fermentation bottle is increased by 45.36 percent compared with the volume of the residual liquid in the common air blowing fermentation culture, and the biomass of hypha is increased by 28.09 percent, thereby having obvious advantages.

The utility model discloses an use is not restricted to above-mentioned embodiment, also is applicable to the cultivation of other microorganism and the humidification under the fermentation cylinder culture condition simultaneously. And simultaneously, the utility model has the advantages of easy operation, long service life.

Claims (3)

1. A sterile ultrasonic humidifying device for air blowing fermentation is characterized by comprising a water filling port, a water storage tank, a water level controller, an atomizing tank, an air outlet, a water level line, an ultrasonic atomizing module and an air inlet; the top of the water storage tank is provided with a water filling port, and the bottom of the water storage tank is connected with the atomization tank; the left side and the right side of the atomization box are respectively provided with an air inlet and an air outlet, and a water level controller and an ultrasonic atomization module are distributed in the atomization box.

2. An aseptic ultrasonic humidifying device for aeration fermentation as claimed in claim 1, wherein: the ultrasonic atomization module adopts an ultrasonic atomization sheet with the diameter of 20mm, and the atomization amount per hour can reach 300 milliliters.

3. An aseptic ultrasonic humidifying device for aeration fermentation as claimed in claim 1, wherein: when the water level line is lower than the optimal working water level line of the ultrasonic atomization module, the water level controller is triggered, and water in the water storage tank flows into the atomization tank to ensure the normal operation of the ultrasonic atomization module.

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