CN211771315U - Sterile ultrasonic humidifying device for air blowing fermentation - Google Patents

Abstract

The utility model relates to an aseptic ultrasonic humidification device for air-blowing fermentation, which comprises a water injection port, a water storage tank, a water level controller, an atomization box, an air outlet, a water level line, an ultrasonic atomization module and an air inlet. The top of the water storage tank is provided with a water injection port, and the bottom is connected to the atomization box; the left and right sides of the atomization box are respectively provided with an air inlet and an air outlet, and a water level controller and an ultrasonic atomization module are distributed inside the atomization box; the ultrasonic mist The atomization module adopts an ultrasonic atomizing sheet with a diameter of 20mm, and the atomization volume can reach 300 ml per hour. The utility model has the advantages of simple structure and convenient use, and can continuously provide saturated steam when it is used for air-blowing fermentation and cultivation of microorganisms, thereby slowing down the evaporation speed of the fermentation liquid in the fermentation bottle, maintaining the osmotic pressure and acid-base balance of the fermentation liquid, and satisfying the needs of different microorganisms. The requirement of fermentation culture time increases biomass; compared with the existing ultrasonic humidification device, the utility model has low production cost, and can be used in connection with an air pump, a microporous filter membrane, and the like.

Description

A sterile ultrasonic humidification device for air-blowing fermentation

technical field

The utility model relates to an ultrasonic humidification device, in particular to a sterile ultrasonic humidification device used for blowing, fermenting and culturing microorganisms.

Background technique

The air blowing method is a way of microbial cultivation in the laboratory, which can increase the biomass of liquid fermentation cultivation, but in the actual cultivation process, with the increase of cultivation time, the fermentation liquid in the fermentation bottle gradually decreases due to evaporation The changes of osmotic pressure, pH, and nutrient concentration affect the growth and reproduction of microorganisms. Therefore, it is very important to maintain the relative humidity in the fermentation bottle during the microbial cultivation process. In order to avoid contamination of the fermentation broth, it is necessary to ensure that the saturated water vapor introduced into the fermentation bottle does not contain impurities when carrying out the microbial fermentation test by blowing air. However, although the existing ultrasonic humidifiers are of various types, they can only be used independently and cannot be used. It can be used in connection with air pump, filter membrane, etc. Therefore, the existing ordinary ultrasonic humidifier cannot be used for such experiments.

Utility model content

The purpose of the utility model is to utilize the ultrasonic humidification device to increase the relative humidity in the fermentation bottle in the process of blowing fermentation and cultivating microorganisms.

The structure of the utility model includes: a water injection port 1 , a water storage tank 2 , a water level controller 3 , an atomization box 4 , an air outlet 5 , a water level line 6 , an ultrasonic atomization module 7 and an air inlet 8 .

When using the utility model, water is injected into the water storage tank 2 from the water injection port 1, then the air inlet 8 is connected to the air pump through a rubber tube, and the air outlet 5 is connected to the microporous filter membrane and then connected to the rubber tube and inserted into the fermentation bottle. After the power supply is turned on, the air pump blows air into the air inlet 8, the ultrasonic atomization module 7 continues to work, and the saturated water vapor generated passes through the microporous filter membrane from the air outlet 5 with the air flow and enters the fermentation bottle to maintain the fermentation of microorganisms. required humidity. When the water level line 6 is about 2-4 cm higher than the ultrasonic atomization module 7, the ultrasonic atomization module 7 has the best working effect. When the water level line 6 is lower, the water level controller 3 is triggered, and the water in the water storage tank 2 flows into In the atomization box 4, to ensure the normal operation of the ultrasonic atomization module 7.

Compared with the existing invention, the utility model has the following advantages:

(1) The humidification device has a simple structure and is easy to use;

(2) The ultrasonic atomization module in the ultrasonic humidification device adopts an ultrasonic atomization sheet with a diameter of 20mm, and the atomization volume per hour can reach 300 ml;

(3) Both sides of the ultrasonic humidification device can be connected with devices such as air pump and microporous membrane, which solves the problem that ordinary humidifiers cannot be combined with other experimental instruments;

(4) The water level controller in the ultrasonic humidification device can automatically control the water volume in the atomization box to ensure the normal operation of the ultrasonic atomization module;

(5) When the ultrasonic humidifier is used for air-blowing fermentation and cultivation of microorganisms, it can continuously provide saturated water vapor, thereby slowing down the evaporation rate of the fermentation liquid in the fermentation bottle, maintaining the osmotic pressure balance of the fermentation liquid, and meeting the different requirements of different microorganisms for fermentation time. , while increasing biomass;

Description of drawings

Fig. 1 is the overall structure schematic diagram of ultrasonic humidification device;

FIG. 2 is a schematic top view of the inside of the atomizing box in the ultrasonic humidifying device.

In the figure, 1 water injection port, 2 water storage tank, 3 water level controller, 4 atomization box, 5 air outlet, 6 water level line, 7 ultrasonic atomization module, 8 air inlet

Detailed ways

The description is as follows in conjunction with the accompanying drawings:

Take the cultivation of Chinese Sanwei ghost pen by blowing air fermentation as an example.

Fill water into the water storage tank 2 from the water injection port 1, then connect the air inlet 8 to the air pump through a rubber tube, and connect the air outlet 5 to the microporous filter membrane and then connect the rubber tube, and insert it into a 3L fermentation bottle (the fermentation bottle contains 1.5 L liquid medium and has been pre-inoculated with Chinese Sanwei ghost pen). After the system is powered on, the ultrasonic atomization module 7 continues to work to generate saturated water vapor. The air pump blows air into the air inlet 8, blows the saturated water vapor out from the air outlet 5, and enters the fermentation bottle after passing through the microporous filter membrane to maintain the Zhonghua powder. Humidity required for fermenting and cultivating tail ghost pen. When the water level line 6 is about 2-4cm higher than the ultrasonic atomization module 7, the ultrasonic atomization module 7 has the best working effect. When the water level line 6 is lower, the water level controller 3 is triggered, and the water in the water storage tank 2 flows into the mist. In the atomizing box 4, to ensure the normal operation of the ultrasonic atomization module 7. Using the utility model to carry out air-blowing fermentation and cultivation of Chinese Sanwei ghost pen is compared with ordinary air-blowing fermentation cultivation, and the results are shown in the following table.

When using the utility model to carry out air-blowing fermentation and cultivation of Chinese Sanwei ghost pen, the remaining liquid volume in the fermentation bottle is increased by 45.36% compared with that of ordinary air-blowing fermentation and cultivation, and the mycelial biomass is increased by 28.09%, which has obvious advantages.

The application of the present invention is not limited to the above-mentioned embodiments, and is also applicable to the cultivation of other microorganisms and the humidification under the cultivation conditions of the fermenter. Meanwhile, the utility model has the advantages of simple operation and long service life.

Claims (3)

1. A sterile ultrasonic humidifying device for air blowing fermentation is characterized by comprising a water filling port, a water storage tank, a water level controller, an atomizing tank, an air outlet, a water level line, an ultrasonic atomizing module and an air inlet; the top of the water storage tank is provided with a water filling port, and the bottom of the water storage tank is connected with the atomization tank; the left side and the right side of the atomization box are respectively provided with an air inlet and an air outlet, and a water level controller and an ultrasonic atomization module are distributed in the atomization box.

2. An aseptic ultrasonic humidifying device for aeration fermentation as claimed in claim 1, wherein: the ultrasonic atomization module adopts an ultrasonic atomization sheet with the diameter of 20mm, and the atomization amount per hour can reach 300 milliliters.

3. An aseptic ultrasonic humidifying device for aeration fermentation as claimed in claim 1, wherein: when the water level line is lower than the optimal working water level line of the ultrasonic atomization module, the water level controller is triggered, and water in the water storage tank flows into the atomization tank to ensure the normal operation of the ultrasonic atomization module.

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