CN206052035U - A kind of fully-automatic intelligent cell culture system - Google Patents
A kind of fully-automatic intelligent cell culture system Download PDFInfo
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- CN206052035U CN206052035U CN201620959292.6U CN201620959292U CN206052035U CN 206052035 U CN206052035 U CN 206052035U CN 201620959292 U CN201620959292 U CN 201620959292U CN 206052035 U CN206052035 U CN 206052035U
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Abstract
This utility model is related to technical field of cell culture, more particularly to a kind of fully-automatic intelligent cell culture system, it includes controller, the first governor motion, the second governor motion, the 3rd governor motion, culturing rack, the first control door, the second control door, the first pipetting device, the second pipetting device, waste collection mechanism, switch bottle mechanism and filling mechanism, and storage intracavity is provided with least three cavitys, including storage chamber, adapter cavity and preheating cavity.Change using this culture apparatuses that liquid is convenient, fast, accurate and cell growth does not affect, and preheat that convenient and preheating speed is very fast.
Description
Technical field
This utility model is related to technical field of cell culture, more particularly to a kind of fully-automatic intelligent cell culture system.
Background technology
In RESEARCH ON CELL-BIOLOGY field and life science field, cell culture is most basic link.Cell
Culture is, cell under artificial environment, using nutrient substance etc., to be bred increment, and for the culture of " various purposes "
Journey.
Prior art cell culture needs to be divided to two environment, and one is culture fluid storage environment in low temperature, culture fluid
Mainly include culture medium, Digestive system and buffer, one is cell culture environment in preference temperature, both prior arts
All it is provided separately, when needing to carry out cultivating fluid exchange or had digestive transfer culture, needs operator manually from one of them
The culture jar of storage culture fluid is taken out in culture environment, Tissue Culture Flask is taken out from another culture environment, then work again
People carries out the replacing of culture fluid by instrument, and culture jar and Tissue Culture Flask are put back into respective culture respectively again finally
In environment, such mode of operation has the disadvantages that：1st, adopt manual operation, it is difficult to the consumption of precise control culture fluid；2、
Manual operation needs for Tissue Culture Flask to take out operation from culture environment, may affect the growth of cell, to the standard tested
Really property produces certain impact；3rd, manual operation is adopted, so culture fluid exchange is less efficient, and is easily malfunctioned；4th, handss are adopted
Dynamic operation, it is impossible to the arbitrarily selected time for changing culture fluid, it is desirable to have can just be changed during operator.
And the culture fluid of prior art be typically all carried out with air as medium it is thermoregulator, so when need preheating
During culture fluid, need culture fluid taking-up is placed under preheating environment, it is so not very convenient, and because be with air as matchmaker
It is situated between, so preheating speed is slower.
Utility model content
A technical problem to be solved in the utility model is：There is provided one kind, to change liquid convenient, fast, accurate and to cell
Growth is without impact, and preheats convenient and preheating speed fully-automatic intelligent cell culture system faster.
The technical scheme adopted by this utility model is：A kind of fully-automatic intelligent cell culture system, it includes control
Device processed, at least provided with storage chamber, culture chamber, operating cavity and observation four chambers in chamber main body, be arranged on culture intracavity be used for
Adjust the first governor motion of culture chamber ambient condition, operation intracavity is arranged on for adjusting the second tune of operating cavity ambient condition
Section mechanism, be arranged on observation intracavity be used for adjust observation chamber ambient condition the 3rd governor motion, be arranged on culture intracavity be used for
The culturing rack of Tissue Culture Flask is placed, and is arranged on culture chamber and the first control door observed between chamber, observation chamber is arranged on behaviour
Make the between chamber second control door, culture intracavity is arranged on for picking and placeing Tissue Culture Flask and driving Tissue Culture Flask to move to
The first pipetting device that observation chamber is observed, it is arranged on operation intracavity and is used to capture the cell brought of the first pipetting device
Culture bottle and control culture bottle carry out action the second pipetting device, be arranged on operation intracavity for collect from Tissue Culture Flask
In pour out waste liquid waste collection mechanism, be arranged on operation intracavity be used to switching the switch bottle mechanism of cell culture bottle cap with
And be arranged on operation intracavity for the filling mechanism to Tissue Culture Flask liquid feeding, the first control door, the second control door, first
Pipetting device, the second pipetting device, waste collection mechanism, switch bottle mechanism, filling mechanism, the first governor motion, second are adjusted
Mechanism and the 3rd governor motion are electrically connected with the controller, and the storage intracavity is provided with least three cavitys, including storage chamber,
Adapter cavity and preheating cavity, the storage chamber, adapter cavity and preheating intracavity are equipped with for housing the container of liquid ethanol, are deposited
Liquid ethanol and the temperature control device for adjusting liquid ethanol temperature, institute are provided with the container for putting chamber and preheating intracavity
It is provided with the container for stating storage intracavity for housing the culture medium Bottle of culture medium, the container of the storage intracavity passes through respectively
The reservoir of conduit and preheating cavity and transition intracavity, and conduit is provided with for controlling in conduit break-make and control conduit
The controlling pump of portion liquid ethanol flow direction, the culture medium Bottle are connected with filling mechanism, the temperature control device and control
Pump processed is electrically connected with the controller.
The Tissue Culture Flask is provided with the label with Quick Response Code, and the culture intracavity is additionally provided with for recognizing that cell is trained
The barcode scanning mechanism of the Quick Response Code on foster bottle label, the barcode scanning mechanism are electrically connected with the controller.
First pipetting device include being arranged on culture intracavity the first track, be arranged on the conveyer belt of observation intracavity with
And the first mechanical hand for being used for pick-and-place Tissue Culture Flask, first mechanical hand and controller electricity are slidably connected on the first track
Connection；Second pipetting device includes being arranged on the second track of operation intracavity and being slidably connected on the second track being used for
Pick-and-place Tissue Culture Flask and the second mechanical hand for driving Tissue Culture Flask execution action, second mechanical hand and controller
Electrical connection.
The photographic head for positioning mechanical hand position is equipped with first mechanical hand and the second mechanical hand, and it is described
Photographic head is electrically connected with the controller.
It is described observation intracavity be provided with for Tissue Culture Flask place transparent mounting table and be arranged under transparent mounting table
It is in observation Tissue Culture Flask inner cell growing state and the observation imaging mechanism of detection Tissue Culture Flask inner cell quantity, described
Observation imaging mechanism is electrically connected with the controller.
The storage chamber includes culture medium storage chamber, buffer storage chamber and Digestive system storage chamber, and three storage chambers
The temperature control device for being equipped with liquid ethanol and adjusting liquid ethanol temperature is inside equipped with container, and container, and is cultivated
Culture medium Bottle is provided with the container of base storage chamber, buffer Bottle, Digestive system in the container of buffer storage chamber, is provided with
Digestive system Bottle is provided with the container of storage chamber, transition intracavity is provided with three containers, and the storage of culture medium storage chamber, buffer
The container of chamber and Digestive system storage intracavity is deposited by conduit three reservoirs respectively with adapter cavity, and culture medium
Chamber, buffer storage chamber and Digestive system are deposited the container of intracavity also by conduit and preheat the reservoir of intracavity, and conduit
On be equipped with for controlling the controlling pump of conduit break-make and control catheter interior liquid ethanol flow direction, the temperature control device
And controlling pump is electrically connected with the controller.
The operation intracavity is provided with for by the uniform piping and druming mechanism of cell piping and druming in Tissue Culture Flask, for accurately taking out
Put part blow and beat the liquid sucting mechanism of uniform cell culture fluid, the first rack placed for Tissue Culture Flask midway, for storing up
Deposit the Tissue Culture Flask of sky the second rack, for place for centrifuge tube the 3rd rack, for place counting chamber the
Four racks, the centrifuge tube handgrip for capturing centrifuge tube, for the centrifuge that is centrifuged to centrifuge tube and for placing discarded
The garbage rack of debris, the piping and druming mechanism, liquid sucting mechanism, centrifuge tube handgrip and centrifuge are electrically connected with controller
Connect, and the switch bottle mechanism is provided with multiple first switch bottle modules for switching cell culture bottle cap and for opening
Close the second switch module of centrifuge tube lid.
Using above structure compared with prior art, this utility model has advantages below：By controller control machinery
Handss complete to change liquid operation, so there is no need to manual operation, the consumption of energy precise control culture fluid；And cell need not be trained
Foster bottle takes out from culture environment and changes liquid again, so the growth of cell will not be subject to too big impact, test accuracy is higher；And
Carry out changing liquid using controller control, error rate is relatively low；As long as and setting and placing enough culture medium and be capable of achieving
Automatically change liquid, it is not necessary to which operator is on the scene in real time, and be provided with observation chamber and be observed before and after liquid is changed, so can guarantee that
The accuracy of test is higher；And be arranged at operating intracavity by most of operation, so do not interfere with culture intracavity culture
Tissue Culture Flask so that test accuracy it is more preferable；And because storage chamber keeping temperature is given using liquid ethanol, but also
Adapter cavity and preheating cavity are provided with, so can be preheated by the flow direction of controlling pump control liquid ethanol, so need not
Culture bottle is taken out, and is preheated using liquid ethanol, preheating speed is very fast.
One barcode scanning mechanism is set, and each Tissue Culture Flask is moved in and out and is required to barcode scanning, such user can be more clear
Chu knows whether each Tissue Culture Flask carried out liquid feeding or had digestive transfer culture, prevents from repeatedly grasping because of user's paramnesia
The situation of work occurs.
And realize Tissue Culture Flask using the cooperation of two mechanical hands and a conveyer belt to be transported to from culture chamber
Observation chamber is transported to operating cavity, such simple structure again, and realizes more convenient.
One photographic head is set on each mechanical hand, and the positioning of such mechanical hand is more accurate so that manipulator behavior
The probability of error is substantially reduced.
One mounting table and observation imaging mechanism are set, cell growth and digestion situation can be so observed, and
The cell quantity obtained in a certain amount of solution can be measured, so that what is distributed during had digestive transfer culture is more accurate.
Storage chamber is divided into into three, is stored culture medium, buffer and Digestive system respectively, and three is arranged in transition intracavity
Individual container, so can be stored into the liquid ethanol of the different temperatures extracted out from different storage chambers in independent container respectively,
The pre-heat effect of so each storage luminal solution is preferable.
And it is also provided with blowing and beating mechanism, liquid sucting mechanism, centrifuge tube handgrip, each rack etc., so that device sheet
Body has had digestive transfer culture function, and had digestive transfer culture function Use Limitation fruit is also preferable so that allomeric function is more diversified.
Description of the drawings
Fig. 1 is a kind of structural representation of fully-automatic intelligent cell culture system of this utility model.
Fig. 2 is a kind of structural representation at fully-automatic intelligent cell culture system another kind visual angle of this utility model.
As shown in the figure：1st, storage chamber；2nd, culture chamber；3rd, operating cavity；4th, observe chamber；5th, culturing rack；6th, the first control door；7、
Second control door；8th, waste collection mechanism；9th, switch bottle mechanism；10th, filling mechanism；11st, the first track；12nd, conveyer belt；13、
First mechanical hand；14th, the second track；15th, the second mechanical hand；16th, transparent mounting table；17th, observe imaging mechanism；18th, blow and beat machine
Structure；19th, liquid sucting mechanism；20th, centrifuge tube handgrip；21st, centrifuge.
Specific embodiment
This utility model is described further with specific embodiment below in conjunction with accompanying drawing, but this utility model is not only
It is limited to detailed description below.
A kind of fully-automatic intelligent cell culture system, it include controller, at least provided with storage chamber 1, culture chamber 2, operation
The main body of chamber 3 and observation 4 four chambers in chamber, it is arranged in culture chamber 2 for adjusting the first regulation of culture chamber ambient condition
Mechanism, it is arranged in operating cavity 3 and is used for for adjusting the second governor motion of operating cavity ambient condition, being arranged in observation chamber 4
Adjust observation chamber ambient condition the 3rd governor motion, be arranged in culture chamber 2 for place Tissue Culture Flask culturing rack 5,
It is arranged on the first control door 6 between culture chamber 2 and observation chamber 4, second be arranged between observation chamber 4 and operating cavity 3 to control
Door 7, it is arranged in culture chamber 2 for picking and placeing Tissue Culture Flask and driving Tissue Culture Flask to move to what observation chamber was observed
First pipetting device, it is arranged in operating cavity 3 for capturing Tissue Culture Flask that the first pipetting device brings and controlling culture
Bottle carry out action the second pipetting device, be arranged in operating cavity 3 for collecting the waste liquid poured out from Tissue Culture Flask
Waste collection mechanism 8, it is arranged in operating cavity 3 for switching the switch bottle mechanism 9 of cell culture bottle cap and being arranged on behaviour
Make in chamber 3 for the filling mechanism 10 to Tissue Culture Flask liquid feeding, the first control door 6, second control door 7, the first liquid relief
Mechanism, the second pipetting device, waste collection mechanism 8, switch bottle mechanism 9, filling mechanism 10, the first governor motion, second are adjusted
Mechanism and the 3rd governor motion are electrically connected with the controller, and are provided with least three cavitys, including storage in the storage chamber 1
Chamber, adapter cavity and preheating cavity, the storage chamber, adapter cavity and preheating intracavity are equipped with for housing the appearance of liquid ethanol
Liquid ethanol and the temperature controller for adjusting liquid ethanol temperature are provided with the container of device, storage chamber and preheating intracavity
Structure, is provided with the container of the storage intracavity for housing the culture medium Bottle of culture medium, the container point of the storage intracavity
Not by the reservoir of conduit and preheating cavity and transition intracavity, and conduit is provided with for controlling conduit break-make and control
The controlling pump of catheter interior liquid ethanol flow direction, the culture medium Bottle are connected with filling mechanism, the temperature control device
And controlling pump is electrically connected with the controller.Controlling pump can be the various pump housings, as long as ethanol flow direction can be controlled.
First adjusting means includes that the first thermoregulation mechanism, the first humidity regulation mechanism, gas concentration lwevel are adjusted
Section mechanism, the first temperature sensor, the first humidity sensor and gas concentration lwevel sensor, second governor motion
Including second temperature governor motion, the second humidity regulation mechanism, second temperature sensor and the second humidity sensor, described
Three governor motions include that the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, three-temperature sensor and the 3rd humidity are passed
Sensor, first thermoregulation mechanism, the first humidity regulation mechanism, the first temperature sensor, the first humidity sensor, second
Thermoregulation mechanism, the second humidity regulation mechanism, gas concentration lwevel governor motion, second temperature sensor, the second humidity are passed
Sensor, gas concentration lwevel sensor, the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, three-temperature sensor and
3rd humidity sensor is electrically connected with the controller.
The Tissue Culture Flask is provided with the label with Quick Response Code, and the culture intracavity is additionally provided with for recognizing that cell is trained
The barcode scanning mechanism of the Quick Response Code on foster bottle label, the barcode scanning mechanism are electrically connected with the controller.Mark is set on Tissue Culture Flask
Sign, and label can be swept to by barcode scanning mechanism, can pass through barcode scanning machine so when culture bottle is put into or takes out culture chamber
Structure confirms.
First pipetting device includes the first track 11 being arranged in culture chamber 2, the transmission being arranged in observation chamber 4
With 12 and be slidably connected on the first track 11 be used for pick-and-place Tissue Culture Flask the first mechanical hand 13, first mechanical hand
13 are electrically connected with the controller；Second pipetting device includes the second track 14 being arranged in operating cavity 3 and is slidably connected
It is used for pick-and-place Tissue Culture Flask and the second mechanical hand 15 for driving Tissue Culture Flask execution action, institute on the second track 14
State the second mechanical hand 15 to be electrically connected with the controller.By the cooperation between two mechanical hands and a conveyer belt, cell is realized
Movement of the culture bottle between operating cavity, observation chamber and culture chamber.
The photographic head for positioning mechanical hand position is equipped with first mechanical hand 13 and the second mechanical hand 15, and
The photographic head is electrically connected with the controller.So can all pass through when needing mechanical hand to need to take out, placing or carry out action
Photographic head is first positioned, then control machinery hand action again.
It is described observation intracavity be provided with for Tissue Culture Flask place transparent mounting table 16 and be arranged under transparent mounting table
For observing the observation imaging mechanism 17 of Tissue Culture Flask inner cell growing state and detection Tissue Culture Flask inner cell quantity,
The observation imaging mechanism 17 is electrically connected with the controller.Observation imaging mechanism can calculate the quantity of cell according to image.
The storage chamber includes culture medium storage chamber, buffer storage chamber and Digestive system storage chamber, and three storage chambers
The temperature control device for being equipped with liquid ethanol and adjusting liquid ethanol temperature is inside equipped with container, and container, and is cultivated
Culture medium Bottle is provided with the container of base storage chamber, buffer Bottle, Digestive system in the container of buffer storage chamber, is provided with
Digestive system Bottle is provided with the container of storage chamber, transition intracavity is provided with three containers, and the storage of culture medium storage chamber, buffer
The container of chamber and Digestive system storage intracavity is deposited by conduit three reservoirs respectively with adapter cavity, and culture medium
Chamber, buffer storage chamber and Digestive system are deposited the container of intracavity also by conduit and preheat the reservoir of intracavity, and conduit
On be equipped with for controlling the controlling pump of conduit break-make and control catheter interior liquid ethanol flow direction, the temperature control device
And controlling pump is electrically connected with the controller.
It is provided with the operating cavity 3 for by the uniform piping and druming mechanism 18 of cell piping and druming in Tissue Culture Flask, for essence
Really extraction part blow and beat the liquid sucting mechanism 19 of uniform cell culture fluid, the first rack placed for Tissue Culture Flask midway,
For store sky Tissue Culture Flask the second rack, for place for centrifuge tube the 3rd rack, for place count
4th rack of plate, the centrifuge tube handgrip 20 for capturing centrifuge tube, for the centrifuge 21 that is centrifuged to centrifuge tube and use
In the garbage rack for placing discarded debris, the piping and druming mechanism 18, liquid sucting mechanism 19, centrifuge tube handgrip 20 and centrifuge
21 are electrically connected with the controller, and the switch bottle mechanism 9 is provided with multiple first opening for switching cell culture bottle cap
Close bottle module and the second switch module for switching centrifuge tube lid.Liquid sucting mechanism 19 is to draw quantitative solution, then
Again the solution of absorption is extruded.Centrifuge tube handgrip 20 is specially to capture centrifuge tube, and he is also have track, can be in track
Upper slip.
A kind of control method of fully-automatic intelligent cell culture system, it includes changing liquid method and had digestive transfer culture method,
The liquid method of changing is comprised the following steps：
1st, controller controls the first mechanical hand crawl Tissue Culture Flask, then passes through controlling pump simultaneously by culture medium storage chamber
Liquid ethanol in container is extracted in adapter cavity container, then the liquid ethanol in preheating cavity container is extracted into culture medium storage chamber appearance
In device, then control the first mechanical hand and move to barcode scanning at barcode scanning mechanism, control afterwards first controls door and opens, and then controls the
One mechanical hand enters into observation intracavity, Tissue Culture Flask is placed on transparent mounting table observes afterwards；
2nd, controller controls the Tissue Culture Flask that the crawl of the first mechanical hand is placed on transparent mounting table, then trains cell
Foster bottle is placed on conveyer belt, is controlled the first mechanical hand afterwards and is returned to culture chamber, while control first controls door closing, conveyer belt
Tissue Culture Flask is transported to below the second control door, then control second controls door opening, controls the second mechanical hand afterwards and stretches
Enter to observe chamber crawl Tissue Culture Flask, then control the second Arm expanding observation chamber, simultaneously close off the second control door；
3rd, the second mechanical hand of controller control is moved at switch bottle mechanism, and then controlling switch bottle mechanism backs out cell training
The bottle cap of foster bottle；
4th, the second mechanical hand of controller control is moved at waste collection mechanism, is then controlled the second mechanical hand and is rotated further
Pour the waste liquid in Tissue Culture Flask into waste collection mechanism；
5th, the second mechanical hand of controller control is moved at filling mechanism, then controls filling mechanism in Tissue Culture Flask
The culture medium that had been warmed up of addition, and after adding culture medium to finish, by controlling pump by the liquid in culture medium storage chamber container
State ethanol is extracted in preheating cavity container, then the liquid ethanol in adapter cavity container is extracted in culture medium storage chamber container；
6th, controller controls the second mechanical hand and moves at switch bottle mechanism, then controlling switch bottle mechanism by bottle cap again
Screw back on Tissue Culture Flask；
7th, controller control second controls door and opens, and then controls the second mechanical hand and stretches into observation chamber, and by cell culture
Bottle is placed into conveyer belt, and the second Arm expanding observation chamber simultaneously closes off the second control door, and the Tape movement of control transmission afterwards will
Tissue Culture Flask is moved, while opening first controls door, controls the first mechanical hand and stretch into observation chamber, and capture conveyer belt to transmit
The Tissue Culture Flask for coming, and Tissue Culture Flask is placed into into observation on transparent mounting table；
8th, controller controls the Tissue Culture Flask that the crawl of the first mechanical hand is placed on transparent mounting table, then controls first
Mechanical hand stretches into culture chamber, controls the first mechanical hand afterwards and takes Tissue Culture Flask at barcode scanning mechanism barcode scanning, finally controls
One mechanical hand is placed into Tissue Culture Flask on the culturing rack of original taking-up, simultaneously closes off the first control door；
And the first mechanical hand or the second mechanical hand are required to be arranged on the first mechanical hand when moving every time in above-mentioned steps
Or the second photographic head positioning on mechanical hand.
The had digestive transfer culture method is comprised the following steps：
A, controller control the first mechanical hand crawl Tissue Culture Flask, while by controlling pump by culture medium storage chamber, slow
Liquid ethanol in rushing liquid storage chamber and Digestive system storage chamber inner pressurd vessel is extracted in three containers of transition intracavity, afterwards respectively
Liquid ethanol in preheating cavity container is extracted into into culture medium storage chamber, buffer storage chamber and Digestive system storage chamber content respectively
In device, then the first mechanical hand moves to barcode scanning at barcode scanning mechanism, and then control first controls door opening, and the first mechanical hand is entered
Tissue Culture Flask is placed into the growing state that transparent mounting table observes Tissue Culture Flask in observation chamber, and the first mechanical hand will be thin afterwards
Born of the same parents' culture bottle is placed on conveyer belt, and then the first mechanical hand stretches back culture chamber, while control first controls door closing；
B, controller control second control door and open, and then control the second mechanical hand and stretch into observation chamber crawl conveyer belt transmission
The Tissue Culture Flask for coming over, then the second mechanical hand stretch back operating cavity, while control second control door close, then control second
Mechanical hand to be moved to and open bottle cap at switch bottle mechanism, then moves to rotation at waste collection mechanism and pours out waste liquid, Ran Houyi
Move filling mechanism and add the buffer having been warmed up；
C, controller control the second mechanical hand and rock Tissue Culture Flask, make buffer abundant with the cell in Tissue Culture Flask
Contact, then controls the second mechanical hand and to move to pour out waste liquid at the rotation of waste collection mechanism；
D, controller control the second mechanical hand and move to filling mechanism and add preheated buffer again, then control the
Two mechanical hands rock Tissue Culture Flask, buffer is fully contacted with the cell in Tissue Culture Flask, and then control second is mechanical
Handss move to rotation at waste collection mechanism and pour out waste liquid；
E, the second mechanical hand of control move to the preheated Digestive system of addition at filling mechanism, move to switch bottle machine afterwards
Bottle cap is shut at structure, the second mechanical hand is then controlled and is rocked Tissue Culture Flask, Digestive system is filled with the cell in Tissue Culture Flask
Tap is touched, and is opened second and is controlled door, and the second mechanical hand of control stretches into observation chamber, while controlling the second mechanical hand by Tissue Culture Flask
Conveyer belt is placed into, while control first controls door opening, the first mechanical hand stretches into what crawl conveyer belt in observation chamber sent
Tissue Culture Flask is placed into observation digestion situation on transparent mounting table, and when observation imaging mechanism observes that digestion is redirected after terminating
To next step, otherwise continue observation；
F, controller control the first mechanical hand Tissue Culture Flask for being placed on transparent mounting table of crawl and place it into
On conveyer belt, control conveyer belt work, while the first mechanical hand stretches back culture chamber, while control first controls door closing, second
Mechanical hand stretches into the Tissue Culture Flask that crawl conveyer belt in observation chamber sends, and controls the second Arm expanding observation chamber afterwards,
Control simultaneously second controls door and closes；
G, controller control the second mechanical hand and move to opening bottle cap at switch bottle mechanism, and the second mechanical hand of control is moved to
Preheated culture medium is added at filling mechanism, the second mechanical hand is then controlled and is moved at piping and druming mechanism in Tissue Culture Flask
Solution blown and beaten, enable bottom of bottle adhesion cell mix homogeneously with solution；
H, the handgrip crawl of control centrifuge tube are placed on the centrifuge tube on the 3rd rack, then control the movement of centrifuge tube handgrip
The lid of centrifuge tube is opened at switch bottle mechanism, the second mechanical hand is controlled afterwards and is poured the solution in Tissue Culture Flask into centrifugation
In pipe, then control the second mechanical hand and Tissue Culture Flask is placed on garbage rack, control centrifuge tube handgrip is moved to
Lid is shut at switch bottle mechanism, centrifuge tube handgrip is put into centrifuge tube in centrifuge and is centrifuged afterwards；
I, centrifugation control centrifuge tube handgrip after terminating and take out centrifuge tube, are moved at switch bottle mechanism and open lid, so
After move to rotation at waste collection mechanism and pour out waste liquid, afterwards centrifuge tube handgrip is moved to add at filling mechanism it is preheated
Culture medium, then again by centrifuge tube handgrip move to piping and druming mechanism at carry out piping and druming mixing；
J, control centrifuge tube handgrip are moved at liquid sucting mechanism, control the solution that liquid sucting mechanism draws X amounts in centrifuge tube, it
The counting chamber that the crawl of the second mechanical hand is placed on the 4th rack is controlled afterwards, then controls liquid sucting mechanism by the X amounts drawn
Liquid is expressed on counting chamber；
K, controller control second control door and open, and the second mechanical hand of control stretches into observation chamber, then places counting chamber
To on conveyer belt, control transmission Tape movement, control afterwards first control door and open, and the first mechanical hand stretches into observation chamber, then grabs
Take the counting chamber on conveyer belt and counting chamber is placed on transparent mounting table using on observation imaging mechanism count plate
Cell quantity；
L, the first mechanical hand crawl counting chamber are placed on conveyer belt, conveyer belt transmission, the second mechanical hand crawl conveyer belt
The counting chamber for sending, is then put into this counting chamber on garbage rack；
M, controller measure the cell quantity in solution according to the X that step K is obtained, and need in obtaining each empty Tissue Culture Flask
The amount of concrete solution to be distributed, then controls centrifuge tube handgrip and grasps centrifuge tube and move at liquid sucting mechanism, and control afterwards is inhaled
Liquid mechanism draws the solution in centrifuge bottle, and the amount of absorption is to need what is distributed in each empty Tissue Culture Flask that controller is obtained
The amount of concrete solution, then controls the Tissue Culture Flask that the crawl of the second mechanical hand is placed on the second rack overhead, control second
Mechanical hand moves to the bottle cap that empty Tissue Culture Flask is opened at switch bottle mechanism, then controls the second mechanical hand and moves to liquid feeding
Preheated culture medium is added in empty Tissue Culture Flask at mechanism, the second mechanical hand is controlled afterwards and is moved to liquid sucting mechanism
Place, then controls liquid sucting mechanism and the solution of absorption is squeezed in Tissue Culture Flask, control the second mechanical hand afterwards and move to out
The bottle cap of Tissue Culture Flask is closed at Guan Ping mechanisms；
N, the second mechanical hand of control stretch into observation chamber, and then Tissue Culture Flask is placed on conveyer belt, conveyer belt is controlled
Transmission, controls the first mechanical hand crawl Tissue Culture Flask for sending of conveyer belt afterwards and is placed into transparent mounting table observation, so
The Tissue Culture Flask that crawl is placed on transparent mounting table afterwards stretches into culture chamber, and the first mechanical hand of control is moved at barcode scanning mechanism
Barcode scanning, is then placed into Tissue Culture Flask on culturing rack；
O, circulation step M-N, the solution in centrifuge tube have had been assigned to the cell training of the sky for setting quantity
After in foster bottle, and after newly adding the Tissue Culture Flask of solution to have been placed within cultivating the culturing rack of intracavity, control the
The centrifuge tube for having taken solution is placed into garbage rack by two mechanical hands, then end operation；
And the first mechanical hand or the second mechanical hand are required to be arranged on the first mechanical hand when moving every time in above-mentioned steps
Or the second photographic head positioning on mechanical hand.
Claims (7)
1. a kind of fully-automatic intelligent cell culture system, it is characterised in that：It include controller, at least provided with storage chamber (1), training
Foster chamber (2), the main body of operating cavity (3) and observation (4) four chambers in chamber, it is arranged in culture chamber (2) for adjusting culture chamber
First governor motion of ambient condition, be arranged in operating cavity (3) for adjust operating cavity ambient condition the second governor motion,
It is arranged in observation chamber (4) for adjusting the 3rd governor motion of observation chamber ambient condition, being arranged in culture chamber (2) for putting
The culturing rack (5) of Tissue Culture Flask is put, culture chamber (2) and the first control door (6) observed between chamber (4) is arranged on, is arranged on
Between observation chamber (4) and operating cavity (3) second controls door (7), is arranged in culture chamber (2) for picking and placeing Tissue Culture Flask simultaneously
And drive Tissue Culture Flask to move to the first pipetting device that observation chamber is observed, be arranged in operating cavity (3) for capturing
Tissue Culture Flask that first pipetting device is brought simultaneously controls culture bottle and carries out the second pipetting device of action, is arranged on operating cavity
(3) the waste collection mechanism (8) for collecting the waste liquid poured out from Tissue Culture Flask in, it is arranged in operating cavity (3) and uses
In switch cell culture bottle cap switch bottle mechanism (9) and be arranged in operating cavity (3) for giving Tissue Culture Flask liquid feeding
Filling mechanism (10), it is described first control door (6), second control door (7), the first pipetting device, the second pipetting device, waste liquid
Collecting mechanism (8), switch bottle mechanism (9), filling mechanism (10), the first governor motion, the second governor motion and the 3rd are adjusted
Mechanism is electrically connected with the controller, and is provided with least three cavitys in the storage chamber (1), including storage chamber, adapter cavity and pre-
Hot chamber, the storage chamber, adapter cavity and preheating intracavity are equipped with for housing the container of liquid ethanol, storage chamber and preheating
Liquid ethanol and the temperature control device for adjusting liquid ethanol temperature are provided with the container of intracavity, the storage intracavity
It is provided with container for housing the culture medium Bottle of culture medium, the container of the storage intracavity passes through conduit and preheating cavity respectively
And the reservoir of transition intracavity, and conduit be provided with for control conduit break-make and control catheter interior liquid ethanol stream
To controlling pump, the culture medium Bottle connected with filling mechanism, the temperature control device and controlling pump with control
Device is electrically connected.
2. a kind of fully-automatic intelligent cell culture system according to claim 1, it is characterised in that：The Tissue Culture Flask
The label with Quick Response Code is provided with, the culture intracavity is additionally provided with for recognizing sweeping for the Quick Response Code on cell culture bottle label
Ink recorder structure, the barcode scanning mechanism are electrically connected with the controller.
3. a kind of fully-automatic intelligent cell culture system according to claim 1, it is characterised in that：The first liquid relief machine
Structure includes the first track (11) being arranged in culture chamber (2), the conveyer belt (12) being arranged in observation chamber (4) and the company of slip
Being connected on the first track (11) is used for first mechanical hand (13) of pick-and-place Tissue Culture Flask, first mechanical hand (13) and control
Device is electrically connected；Second pipetting device includes the second track (14) being arranged in operating cavity (3) and is slidably connected at
It is used for pick-and-place Tissue Culture Flask and the second mechanical hand (15) for driving Tissue Culture Flask execution action, institute on two tracks (14)
State the second mechanical hand (15) to be electrically connected with the controller.
4. a kind of fully-automatic intelligent cell culture system according to claim 3, it is characterised in that：First mechanical hand
(13) photographic head for positioning mechanical hand position, and the photographic head and controller and on the second mechanical hand (15) are equipped with
Electrical connection.
5. a kind of fully-automatic intelligent cell culture system according to claim 1, it is characterised in that：The observation intracavity sets
Having the transparent mounting table (16) placed for Tissue Culture Flask and being arranged under transparent mounting table is used to observe in Tissue Culture Flask
The observation imaging mechanism (17) of cell growth status and detection Tissue Culture Flask inner cell quantity, the observation imaging mechanism
(17) it is electrically connected with the controller.
6. a kind of fully-automatic intelligent cell culture system according to claim 1, it is characterised in that：The storage chamber includes
Culture medium storage chamber, buffer storage chamber and Digestive system storage chamber, and three storage intracavity be equipped with container, and container
It is provided with liquid ethanol and adjusts the temperature control device of liquid ethanol temperature, and in the container of culture medium storage chamber, is provided with culture
Base Bottle, is provided with buffer Bottle, is provided with Digestive system in the container of Digestive system storage chamber in the container of buffer storage chamber
Bottle, transition intracavity are provided with three containers, and culture medium storage chamber, buffer storage chamber and Digestive system deposit the appearance of intracavity
Device passes through conduit three reservoirs respectively with adapter cavity, and culture medium storage chamber, buffer storage chamber and Digestive system
Storage intracavity container also by conduit with preheating intracavity reservoir, and be equipped with conduit for control conduit break-make with
And the controlling pump that catheter interior liquid ethanol is flowed to is controlled, the temperature control device and controlling pump are electrically connected with controller
Connect.
7. a kind of fully-automatic intelligent cell culture system according to claim 1, it is characterised in that：The operating cavity (3)
Inside it is provided with uniform for blowing and beating by the uniform piping and druming mechanism (18) of cell piping and druming in Tissue Culture Flask, for accurate extraction part
Cell culture fluid liquid sucting mechanism (19), for Tissue Culture Flask midway place the first rack, for store sky cell
Second rack of culture bottle, the 3rd rack for placing for centrifuge tube, the 4th rack for placing counting chamber,
Centrifuge tube handgrip (20) in crawl centrifuge tube, for the centrifuge (21) that is centrifuged to centrifuge tube and for placing discarded debris
Garbage rack, the piping and druming mechanism (18), liquid sucting mechanism (19), centrifuge tube handgrip (20) and centrifuge (21) with
Controller is electrically connected, and switch bottle mechanism (9) is provided with multiple first switch bottles for switching cell culture bottle cap
Module and the second switch module for switching centrifuge tube lid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620959292.6U CN206052035U (en) | 2016-08-29 | 2016-08-29 | A kind of fully-automatic intelligent cell culture system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620959292.6U CN206052035U (en) | 2016-08-29 | 2016-08-29 | A kind of fully-automatic intelligent cell culture system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367343A (en) * | 2016-08-29 | 2017-02-01 | 宁波键生物科技有限公司 | Full-automatic intelligent cell culture device and control method thereof |
| CN108085253A (en) * | 2017-12-30 | 2018-05-29 | 宁波华仪宁创智能科技有限公司 | Embryo Culture and operation integrated apparatus and its method of work |
| CN110129192A (en) * | 2019-05-27 | 2019-08-16 | 傅兰轲 | A kind of full-automatic cell is separately cultured system |
| CN110640765A (en) * | 2019-08-20 | 2020-01-03 | 辽宁成大生物股份有限公司 | Operating system |
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2016
- 2016-08-29 CN CN201620959292.6U patent/CN206052035U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106367343A (en) * | 2016-08-29 | 2017-02-01 | 宁波键生物科技有限公司 | Full-automatic intelligent cell culture device and control method thereof |
| CN106367343B (en) * | 2016-08-29 | 2018-09-21 | 杭州键一生物科技有限公司 | A kind of fully-automatic intelligent cell culture apparatus and its control method |
| CN108085253A (en) * | 2017-12-30 | 2018-05-29 | 宁波华仪宁创智能科技有限公司 | Embryo Culture and operation integrated apparatus and its method of work |
| CN110129192A (en) * | 2019-05-27 | 2019-08-16 | 傅兰轲 | A kind of full-automatic cell is separately cultured system |
| CN110129192B (en) * | 2019-05-27 | 2022-05-31 | 傅兰轲 | Full-automatic cell separation culture system |
| CN110640765A (en) * | 2019-08-20 | 2020-01-03 | 辽宁成大生物股份有限公司 | Operating system |
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