CN203007262U - Microarray bacterium recognition and screening device - Google Patents
Microarray bacterium recognition and screening device Download PDFInfo
- Publication number
- CN203007262U CN203007262U CN 201320020581 CN201320020581U CN203007262U CN 203007262 U CN203007262 U CN 203007262U CN 201320020581 CN201320020581 CN 201320020581 CN 201320020581 U CN201320020581 U CN 201320020581U CN 203007262 U CN203007262 U CN 203007262U
- Authority
- CN
- China
- Prior art keywords
- bacterium
- plane
- microarray
- capture
- catching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
A microarray bacterium recognition and screening device is characterized by comprising a bacterium catcher and a bacterium incubator. The bacterium catcher at least comprises a catching plane and a plurality of bacterium catching pieces arranged on the catching plane. The bacterium incubator at least comprises an incubation plane and a plurality of blind holes which are longitudinally formed in the incubation plane. The bacterium catching pieces and the blind holes are in clearance fit and are in one-to-one correspondence mode. According to the device, the mode that specific antibodies are combined with the bacterium catching pieces is adopted, and efficiency of catching and separating bacteria in a to-be-detected sample is improved. The plurality of bacterium catching pieces are arranged on one plane in order and form an array and are contacted with the to-be-detected sample in a 'seal' mode, synchronism for catching the various bacteria contained in the to-be-detected sample is achieved, and operation efficiency is improved.
Description
Technical field
The utility model relates to a kind of for biomedical proofing unit, relates in particular to a kind of device with the microarray form Design, can be simultaneously the bacterium in identical or different source is identified and sieves, to improve identification and the bioaccumulation efficiency to specific bacteria.
Background technology
Bacterium (bacteria) for belonging to prokaryotic micro-organisms, is one of biological main monoid, and kind is various.Bacterium is distributed widely in soil and water, or with the other biological symbiosis.Numerous disease as: pulmonary tuberculosis, gonorrhoea, anthrax, syphilis, the plague and sand holes etc. are caused by bacterium, simultaneously a lot of food and medicine also rely upon bacterium, as wine, part milk-product, part microbiotic and part biological goods etc.Therefore, for the identification of pathogenic bacterium with separate, be conducive to diagnosis and the treatment of various diseases, for the identification of probiotics with separate, more help people to develop and utilize.
Chinese utility model patent ZL96207784.4 discloses a kind of medical bacterium collection of specimens and culture apparatus, turn the transferred species ring that is coated onto flat board, and form in order to the square die shoe of placing above-mentioned parts by at least one flat board, at least one specimen cup, at least one sample with collection that contains substratum.Flat board, specimen cup and the transferred species ring that contains substratum in square die shoe or upper-lower overlapping place, or place at same plane, and cross the dull and stereotyped center of circle that contains substratum.Square die shoe surface is provided with inwardly corresponding garden cylindrical groove and the long strip shape groove of placing flat board, transferred species ring, specimen cup of recessed, confession; The transferred species ring is made of a cavity ring and the strip handle that is attached thereto.Device radially is provided with a tabula, and flat board is divided into two.This device Main Function is to make the collection of sample clean, sanitary, effectively blocks pipeline and the infection prevention of pathogenic bacterium, but is difficult to effectively separate various bacteria.
Chinese utility model patent ZL200620099239.X discloses a kind of biological specimen automatization plate for isolated culture, comprise a capping and the square culture plate that links into an integrated entity with capping, two sides of square culture plate are equipped with a plane that falls in, be filled with substratum on this plane, the two ends of capping have jack, activity is inserted with forked inoculator in jack, and it is close that the transfering loop of inoculator extends to bottom and the culture plate of square culture plate.During use, nutrient solution contacts with transfering loop in the culturing room bottom, makes transfering loop carry sample.Then the mechanical manipulator of instrumentation will the clamp pilot lever, allows transfering loop carry sample on it, by moving up and down of rule, with the sample streak inoculation to culture plate.Then, the temperature in instrumentation is remained on the 35-37 degree, the growth of the suitable bacterium of this temperature remains to bacterium always and grows on culture plate under this environment.Mobile transfering loop makes the transfering loop that speckles with nutrient solution (sample) be inoculated on the substratum of culture plate according to certain orientation.This culture plate can be realized the separation to various bacteria according to different classes of sample and different substratum, but still needs artificial replaced medium, also unrealized sharp separation various bacteria.
The automatization that separates for improving bacterium, Chinese utility model patent ZL200620163876.9 discloses a kind of automation bacteria separation and Culture instrument, a rotatable sample disk is housed in the centre of thermostat container, the edge of rotatable sample disk is provided with permanent seat, can place culture apparatus special in permanent seat, be provided with a running gear above rotatable sample disk, a device for exerting and automatic marking apparatus are housed on running gear.
in order to solve the sharp separation various bacteria, Chinese utility model patent ZL200720089015.5 discloses a kind of automation bacteria separating culturing instrument with multiple culture mediums, the selection of multiple substratum (as: four kinds of substratum) is provided, a rotatable sample disk is equipped with in its centre at thermostat container, rotatable sample disk is comprised of a plurality of sample racks, the circumferential edges of sample rack is provided with a plurality of sample slot, sample slot has collection tube chamber groove and culturing room's groove two portions of mutual UNICOM, be provided with a running gear above rotatable sample disk, a device for exerting and automatic marking apparatus are housed on running gear, transferring of sample slot is provided with the permanent magnet that can turn round, produce alternating magnetic field under the driving of motor, in the sample collection pipe of the side of being placed on it, little magnetic patch is arranged in the magnetic field of alternation, it is characterized in that: mutual UNICOM between adjacent culturing room's groove, a four sides separation culture device occupies a collection tube chamber groove and two culturing room's grooves.This equipment has been realized the needs that Microbiological Lab cultivates the biological specimen sharp separation, is difficult to be applicable to environmental microorganism is identified with unknown microorganism and separated needs.
The utility model content
The purpose of this utility model is to provide the identification of a kind of microarray bacterium and screening plant, improving identification, separation, screening and the bioaccumulation efficiency of bacterium, with the looking for of the examination that is applicable to pathogenic bacterium and probiotics, development and utilization.
Antigen is the material that can cause specific immune response in body.After antigen enters body, can stimulate body produce antibody and cause cellular immunization.In immunoassay, antigen refer to can with the material of antibodies.The reactivity of antigen depends on antigenic determinant (antigenic determinant), also claims epi-position (epitope).An antigen molecule can be with different determinants.In addition, can also splice epi-position by the mode that genetically engineered or chemistry connect.
Antibody is the immunoglobulin (Ig) (immunoglobulin, Ig) that can be combined with antigen-specific.Ig divides five classes, i.e. IgG, IgA, IgM, IgD and IgE.The Ig relevant with immunoassay is mainly IgG and IgM.
The preparation of antigen or antibody, can synthesize by chemiluminescent polypeptide, prokaryotic micro-organisms (as: intestinal bacteria) genetic engineering bacterium express after purifying, eukaryotic microorganisms (as: cereuisiae fermentum, pichia spp and Kluyveromyces Lactis Wei yeast etc.) genetic engineering bacterium express after purifying or expressed and purifying by zooblast (as: Chinese hamster CHO, hamster BHK, rat bone marrow tumour cell l cell, monkey CV1 cell and human lymphocyte etc.) etc.
A kind of microarray bacterium identification and screening plant that the utility model provides comprise bacterium grabber and incubator.The bacterium grabber comprises a capture plane at least, is located at some bacterium capture pieces of this capture plane.Incubator comprises that at least one is cultivated the plane, cultivates along this some blind holes that plane vertically arranges.Each bacterium capture pieces and each blind hole running fit, corresponding one by one.
The top layer of bacterium capture pieces is combined with antibody layer, carries out combination with specific with bacterium surface antigen.
For making the bacterium that catches can move into incubator, a kind of embodiment that the utility model adopts is, with capture plane and cultivation plane applying.
For making the bacterium that catches can move into incubator, the another kind of embodiment that the utility model adopts is, each bacterium capture pieces is 1/10 hemisphere face-1 hemisphere face, preferentially selects semisphere, and the opening section of each blind hole be circle.
Alleged " antibody layer " of the utility model is interpreted as containing an area of space of the antibody that can be combined with the bacterium surface antigen-specific.For being combined in mode covalently or non-covalently with the bacterium capture pieces, it also comprises other material for connection antibody, as: but be not limited only to vitamin H, the second antigen or second antibody etc.
Blood, secretory product, tissue juice, vitro culture liquid or tissue etc. that " sample to be checked " that the utility model is alleged or " sample " come from soil, water body, experimenter.
Alleged " experimenter " of the utility model refers to people, wildlife and domestic animal (Livestock).Wildlife is without domestication's animal under state of nature.Domestic animal is the animal of the artificial breeding for food source is provided, as: monkey, dog, mouse, hamster, pig, rabbit, milk cow, buffalo, bull, sheep, goat, goose and chicken etc." patient " that diagnose preferentially selects Mammals, especially people.
The beneficial effect that technical solutions of the utility model realize:
The identification of microarray bacterium and screening plant that the utility model provides, the mode of employing binding specificity antibody on the bacterium capture pieces improves capture rate and the separation efficiency for the treatment of bacterium in the sample basis.Some bacterium capture pieces in one plane ordered arrangement form array, contact with sample to be checked in the mode of " seal ", have realized synchronism that various bacteria contained in sample to be tested is caught having improved operation efficiency.Simultaneously, cooperation is in one plane to be provided with the incubator of a plurality of blind holes, multiple substratum (as: liquid, solid and gel) is placed in respectively in each blind hole, realized the synchronism of separation, enrichment and the cultivation of various bacteria, thereby provide basis for estimation in time understanding bacterial species in sample to be checked, genotype, biological nature, pathogenic and meta-bolites etc., also for further understanding and the screening bacteria sample provides material.
Description of drawings
Fig. 1 is the structural representation of the identification of the utility model microarray bacterium and screening plant one embodiment;
Fig. 2 be in Fig. 1 the bacterium grabber look up structural representation.
Embodiment
Describe the technical solution of the utility model in detail below in conjunction with accompanying drawing.The utility model embodiment is only unrestricted in order to the technical solution of the utility model to be described, although with reference to preferred embodiment, the utility model is had been described in detail, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement the technical scheme of utility model, and not breaking away from the spirit and scope of technical solutions of the utility model, it all should be encompassed in claim scope of the present utility model.
Fig. 1 is the structural representation of the utility model microarray bacterium identification and screening plant one embodiment, Fig. 2 be in Fig. 1 the bacterium grabber look up structural representation.As depicted in figs. 1 and 2, this device comprises bacterium grabber 1, and it comprises a capture plane 11 at least, is located at some bacterium capture pieces 12 of this capture plane.Each bacterium capture pieces is semisphere, and ordered arrangement forms one group of capture array (referring to Fig. 2).
Various bacteria culture mediums (as: liquid, solid and gel) add respectively in each blind hole 22, form a microbial culture array that contains multiple substratum.In each blind hole, the material of used medium composition can be identical or different.
Will be as the plane 11 of the bacterium grabber 1 of " seal " towards sample to be checked (as: soil, water body, nutrient solution, juice and tissue juice etc.), and each bacterium capture pieces 12 on it is fully contacted with sample to be checked, the antibody layer antigen-specific that contained bacterium has in sample to be checked of each bacterium capture pieces top layer combination is combined, to catch the bacterium of particular types.Afterwards, bacterium grabber 1 is moved to incubator, capture plane 11 is fitted with cultivating plane 12, makes each bacterium capture pieces 12 and each fill the blind hole 22 corresponding and fully contact one by one of substratum, and the bacterium that is beneficial to combination on each capture pieces moves in the substratum of each blind hole.
For the bacterium of particular types, its requirement to substratum is also different.Only under suitable condition, could cultivate good.Therefore, through cultivation after a while, each blind hole will present different cultivation results, and the bacterial growth sign appears in some blind hole, as: form the visible bacterium colony, or chromogenesis, or the substratum colour developing etc., some is without obvious bacterial growth.Thus, the kind of institute's binding antibody on sign that can be by bacterial growth and bacterium capture pieces is tentatively judged bacterial species contained in sample to be checked, genotype, biological nature, pathogenic and meta-bolites etc.These can further be understood and screening by means of detection meanss such as PCR by the bacterium of enrichment.
Claims (6)
1. a microarray bacterium identifies and screening plant, it is characterized in that comprising
The bacterium grabber comprises a capture plane at least, and some bacterium capture pieces of being located at described capture plane;
Incubator comprises that is at least cultivated a plane, and along the vertical some blind holes that arrange in described cultivation plane;
Each described bacterium capture pieces and each described blind hole running fit, corresponding one by one.
2. microarray bacterium identification according to claim 1 and screening plant, is characterized in that the top layer of described bacterium capture pieces is combined with antibody layer.
3. microarray bacterium identification according to claim 1 and screening plant, is characterized in that each described bacterium capture pieces is 1/10 hemisphere face-1 hemisphere face.
4. microarray bacterium identification according to claim 1 and screening plant, is characterized in that each described bacterium capture pieces is semisphere.
5. microarray bacterium identification according to claim 1 and screening plant, is characterized in that the opening section of each described blind hole is for circular.
6. microarray bacterium identification according to claim 1 and screening plant, is characterized in that described capture plane and described cultivation plane applying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320020581 CN203007262U (en) | 2013-01-15 | 2013-01-15 | Microarray bacterium recognition and screening device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320020581 CN203007262U (en) | 2013-01-15 | 2013-01-15 | Microarray bacterium recognition and screening device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203007262U true CN203007262U (en) | 2013-06-19 |
Family
ID=48598875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320020581 Expired - Fee Related CN203007262U (en) | 2013-01-15 | 2013-01-15 | Microarray bacterium recognition and screening device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203007262U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104115010A (en) * | 2012-01-10 | 2014-10-22 | 生物梅里埃公司 | Method for directly detecting and identifying a microorganism in a biological sample via optical means |
| CN109060743A (en) * | 2018-08-07 | 2018-12-21 | 中国科学院海洋研究所 | A kind of microorganism identification method |
-
2013
- 2013-01-15 CN CN 201320020581 patent/CN203007262U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104115010A (en) * | 2012-01-10 | 2014-10-22 | 生物梅里埃公司 | Method for directly detecting and identifying a microorganism in a biological sample via optical means |
| CN109060743A (en) * | 2018-08-07 | 2018-12-21 | 中国科学院海洋研究所 | A kind of microorganism identification method |
| CN109060743B (en) * | 2018-08-07 | 2021-03-02 | 中国科学院海洋研究所 | Microorganism identification method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Islam et al. | Isolation and identification of Escherichia coli and Salmonella from poultry litter and feed | |
| CN106544270B (en) | A kind of micro-fluidic chip and its cell culture processes co-cultured for cell | |
| CN102746986B (en) | Tumor cell migration dynamics monitoring method based on microfluidic chip | |
| CN206382042U (en) | A kind of biological culture test tube placing box | |
| CN104789500B (en) | A kind of white diarrhea dyeing aggegation antigen and its preparation method and application | |
| CN104450860B (en) | A kind of mycoplasma pneumoniae culture medium | |
| CN203007262U (en) | Microarray bacterium recognition and screening device | |
| CN109706115A (en) | A kind of construction method of Marrow Mesenchymal Stem Cells cell line | |
| CN205797284U (en) | A kind of biotechnology Reagent Tube placement cabinet | |
| CN110484467A (en) | Antibacterial peptide and the application of one plant of bacillus polymyxa and its generation | |
| CN101838626B (en) | Bartonia body solid-liquid double-phase slant culture medium as well as preparation method and application method thereof | |
| CN101659983A (en) | Method for detecting number of viable bacillus | |
| CN102876562A (en) | Microfluidic chip system for culture and multiplication behavior research of marine microalgae | |
| CN104178451B (en) | From milk, separate and cultivate method and the special culture media of cow mammary gland epithelial cells | |
| CN106497854B (en) | Lactobacillus D8 and its application | |
| CN108753596A (en) | A kind of microorganism growth image detection microchamber microfluidic system | |
| CN103160434B (en) | An automatic cell culture device capable of simulating internal environments of organisms | |
| CN109097274A (en) | The standardized high-throughput three-dimensional cell preparation facilities of one kind and its application | |
| CN202626198U (en) | High-throughput cell culture apparatus easy for cell wall removal | |
| CN108018228B (en) | Escherichia coli O157H 7 low virulent strain and application thereof | |
| CN103777009B (en) | A kind of method using horseradish peroxidase labeling antibody to detect the weak malicious virus titer of swine fever | |
| CN207659457U (en) | Batch anaerobic ferment devices | |
| CN207062281U (en) | Sample smear can be carried out is separately cultured device | |
| CN202543220U (en) | High-throughput cell culture incubator | |
| CN109576183A (en) | A kind of siphon guide feed supplement shaking table culture method for bacterium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: WENZHOU MEDICAL UNIVERSITY RENJI COLLEGE Free format text: FORMER OWNER: WENZHOU MEDICINAL INSTITUTE Effective date: 20140526 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 325027 WENZHOU, ZHEJIANG PROVINCE TO: 325000 WENZHOU, ZHEJIANG PROVINCE |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20140526 Address after: 325000 Zhejiang province Chashan Wenzhou Higher Education Park Patentee after: Shanghai Institute of Wenzhou Medical University Address before: 325027 Wenzhou college, Zhejiang, West Road, No. 82 Patentee before: Wenzhou Medical College |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130619 Termination date: 20160115 |
|
| EXPY | Termination of patent right or utility model |