CN207659457U - Batch Anaerobic Fermentation Unit - Google Patents

Abstract

The utility model belongs to the field of laboratory chemical instruments, in particular to a batch type anaerobic fermentation device, comprising a constant temperature water bath and a plurality of anaerobic fermentation units arranged in the constant temperature water bath; the anaerobic fermentation unit includes upper and lower side ends An open fermentation bottle, an exhaust unit connected to the rubber stopper at the upper opening of the fermentation bottle, and a sampling unit connected to the upper and lower side openings respectively; the exhaust unit includes a hard air duct inserted into the rubber stopper and a The soft air tube connected with the hard air tube; the other end of the soft air tube goes deep into the bottom of the measuring cylinder that is filled with saturated saline and placed upside down in the liquid reservoir; the sampling unit includes a soft tube connected to the upper and lower side openings, A three-way tube connected to the soft catheter and a hemostatic clip arranged on the soft catheter; one end of the lower end of the three-way tube is connected to a syringe for extracting fermentation liquid samples; one end of the upper end of the three-way tube is connected to a precision syringe For taking samples of fermentation gases.

Description

Batch Anaerobic Fermentation Unit

technical field

The utility model belongs to the field of laboratory chemical instruments, in particular to a batch type anaerobic fermentation device.

Background technique

The energy utilization model based on biogas fermentation is an important measure for the development of circular agriculture. Biogas is a kind of renewable energy with great development prospects in biomass energy. As a renewable energy that is easy to transport, has a wide range of uses (heating, power and energy), and is cheap, it has many irreplaceable advantages. According to estimates, the total amount of livestock and poultry manure resources in my country is about 850 million tons at present, and if it is converted into biogas, it can be converted into about 78.4 million tons of standard coal. Moreover, with the rapid development of industrial and agricultural production, the continuous expansion of urban scale and the highly intensive and factory-like operation of animal husbandry, the generation of urban sewage and livestock excrement is on the rise, which contains a large amount of heavy metals, bacteria, pathogenic bacteria and viruses, etc. , if it is discharged without treatment, it will seriously affect environmental sanitation and human health; at the same time, sewage and livestock excrement also contain organic matter and nutrients such as N, P, and K required for microbial growth. cause waste of resources. Biogas production can not only effectively improve the above problems, but also alleviate energy shortages, reduce greenhouse gas emissions, and promote sustainable economic and social development.

Biogas fermentation is also called anaerobic digestion and anaerobic fermentation. Under anaerobic conditions, microorganisms decompose organic matter and generate biogas. It refers to the process of organic matter (such as livestock manure, straw, weeds, etc.) Under oxygen conditions, through the catabolism of various types of microorganisms with a large number and different functions, the complex organisms that eventually form mixed gases (methane) such as methane (50-75%) and carbon dioxide (25-50%) chemical process. There are many biogas anaerobic fermentation processes, among which according to the feeding method, they can be divided into: continuous fermentation, batch fermentation, two-step fermentation and batch fermentation. Biogas batch fermentation refers to a fermentation method in which the medium is added at one time and the product is harvested at one time during the fermentation process, and there is no exchange of other materials with the outside world. It is a widely used fermentation method. The method is simple and easy to operate, but it is difficult to detect real-time changes in the gas components produced in this batch anaerobic fermentation system and the nutritional components of the fermentation broth. In order to solve the above problems, it is particularly important to design a device that can simultaneously complete the determination of the above indicators.

Utility model content

The purpose of the utility model is to overcome the shortcomings in the prior art and provide a batch type anaerobic fermentation device.

The technical scheme adopted for realizing the purpose of this utility model is:

A batch anaerobic fermentation device, comprising a constant temperature water bath and a plurality of anaerobic fermentation units arranged in the constant temperature water bath;

The anaerobic fermentation unit includes a fermentation bottle with openings on the upper and lower sides, an exhaust unit connected to the rubber stopper at the opening of the upper end of the fermentation bottle, and a sampling unit connected to the openings on the upper and lower sides respectively; The exhaust unit includes a hard air tube inserted into the rubber stopper and a soft air tube connected to the hard air tube; the other end of the soft air tube goes deep into the tube filled with saturated salt Water is placed upside down at the bottom of the graduated cylinder of the liquid reservoir; the sampling unit includes a soft conduit connected to the upper and lower side openings, a tee connected to the soft conduit, and a tee pipe arranged on the soft conduit The hemostatic clip on the upper side; one end of the three-way tube on the lower side is connected with a liquid extractor for extracting fermentation liquid samples; one end of the three-way tube on the upper side is connected with a closed soft rubber tube for extracting fermentation gas samples. When in use, open the valve of the three-way pipe on the upper side to allow the gas in the fermentation bottle to flow into the closed soft rubber tube at one end, then close the valve, and immediately insert the gas extractor into the closed soft rubber tube at one end to extract the fermentation gas sample. Immediately after the needle hole is blocked with Vaseline, the airtightness of the device can be satisfied.

The constant temperature water bath is provided with an oscillating box; the bottom surface of the oscillating box is provided with a plurality of holes for the passage of the constant temperature liquid; the horizontal middle position of the oscillating box is provided with a moving plate, A plurality of partition boards are arranged longitudinally; the moving board and the plurality of partition boards are separated into a plurality of storage spaces for placing the anaerobic fermentation unit.

The middle position of the fermentation bottle is provided with an annular fixed ring; one end of the fixed ring is fixed on the moving plate, and the other end is fixed on the inner wall of the vibration box through a spring body; the vibration box One side of the body is provided with a slider linked with the moving plate and a slideway matched with the slider, and the slider is connected with a motor to move back and forth.

The peripheral wall of the rubber plug ring is provided with an annular baffle with an opening downward.

The fermentation bottle is a 500ml suction filter bottle with upper and lower side openings and an upper opening, and the liquid extractor is a 5ml disposable syringe. The air extractor is a 1ml precision syringe.

Compared with the prior art, the beneficial effects of the utility model are:

The utility model adopts common laboratory instruments, which are easy to install, and can complete batch fermentation tests of multiple samples in a constant temperature water bath at the same time, and can collect fermentation gas and/or fermentation liquid samples at the same time. The measurement of cumulative gas production is conducive to the convenient, fast and accurate real-time detection and analysis of gas and/or liquid components, and ensures the anaerobic environment of the fermentation system. The utility model selects the suction filter bottle with the upper and lower side ports and the upper end opening as the fermentation bottle, which reduces the contact area with the outside world, not only can fully ensure the anaerobic environment of the system, but also can complete the fermentation of a certain amount of substrate.

In addition, in the present utility model, a vibrating box is added to the constant temperature water bath pot, which can drive the moving plate to move back and forth through the slider while completing the constant temperature water bath, so as to vibrate and shake the fermentation liquid in the fermentation bottle to speed up the fermentation process. Ensure the uniformity and high efficiency of the fermentation system.

Description of drawings

Fig. 1 is the schematic diagram of the anaerobic fermentation unit of the present invention.

Fig. 2 is a schematic diagram of a batch anaerobic fermentation device of the present invention.

Detailed ways

In order to enable those skilled in the art to better understand the technical solution of the utility model, the utility model will be further described in detail below in conjunction with the accompanying drawings and preferred embodiments.

Fig. 1-2 shows a kind of batch type anaerobic fermentation device, comprises constant temperature water bath 2 and a plurality of anaerobic fermentation units 1 arranged in described constant temperature water bath; Described anaerobic fermentation unit comprises upper and lower side end Open fermenter bottles 11, exhaust units 12 connected to the rubber stoppers on the upper openings of the fermentation bottles, and sampling units 13 connected to the upper and lower side openings respectively; The hard airway tube 122 of the rubber stopper 121 and the soft airway tube 123 connected with the hard airway tube; The bottom of the measuring cylinder 124 of the liquid reservoir 125; the surrounding wall of the rubber stopper ring is provided with an annular baffle with an opening downward, and the rubber stopper is a tapered design with a large top and a small bottom. The design of this place can make it difficult for the rubber stopper to be taken out when the hard airway tube is inserted and pulled out, thereby affecting the anaerobic environment. The sampling unit includes a soft conduit 131 connected to the upper and lower side openings, a tee pipe 133 connected to the soft conduit, and a hemostatic clip 132 arranged on the soft conduit; One end of the through pipe is connected with a liquid extractor 134 for extracting fermentation broth samples; the liquid extractor is a 5ml disposable syringe. One end of the three-way pipe on the upper side is connected with an airtight soft rubber tube for extracting fermentation gas samples. When in use, open the valve of the three-way tube on the upper side to allow the gas in the fermentation bottle to flow into the closed soft rubber tube at one end, then close the valve, and immediately insert a syringe into the closed soft rubber tube at one end to extract the fermentation gas sample. Immediately block the hole of the needle with Vaseline to satisfy the airtightness of the device. The syringe used here is a 1ml precision syringe. The fermentation bottle is a 500ml suction filter bottle with upper and lower side openings and upper openings.

The constant temperature water bath is provided with an oscillating box; the bottom surface of the oscillating box is provided with a plurality of holes for the passage of the constant temperature liquid; the horizontal middle position of the oscillating box is provided with a moving plate 141 , a plurality of partition boards 146 are arranged longitudinally; the moving board and the plurality of partition boards are separated into a plurality of storage spaces for placing the anaerobic fermentation unit. The middle position of the fermentation bottle is provided with an annular fixed ring; one end of the fixed ring is fixed on the moving plate through the fixed frame 143, and the other end is fixed on the inner wall of the vibration box through the spring body 142; One side of the vibrating box is provided with a slide block 144 linked with the moving plate and a slideway 145 matched with the slide block, and the slide block is connected with a motor to move back and forth.

In a word, what the utility model adopts are all the commonly used instruments in the laboratory, which are easy to install, and can simultaneously complete batch fermentation tests of multiple samples in a constant temperature water bath, can simultaneously collect fermentation gas and/or fermentation liquid samples, and can also The measurement of the cumulative gas production is conducive to the convenient, fast and accurate real-time detection and analysis of gas and/or liquid components, and ensures the anaerobic environment of the fermentation system. The utility model selects the suction filter bottle with the upper and lower side openings and the upper opening as the fermentation bottle, which reduces the contact area with the outside world, not only can fully ensure the anaerobic environment of the system, but also can complete the fermentation of a certain amount of substrate.

In addition, in the present utility model, an oscillating box is added to the constant-temperature water tank, which can drive the moving plate to move back and forth through the slider while completing the constant-temperature water bath, thereby vibrating and shaking the fermentation liquid in the fermentation bottle, speeding up the fermentation process, and reducing Experiment time, improve efficiency.

The above is only a preferred embodiment of the utility model, it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the utility model, some improvements and modifications can also be made, these improvements and Retouching should also be regarded as the scope of protection of the present utility model.

Claims (5)

1. a kind of batch anaerobic ferment devices, which is characterized in that including thermostat water bath and be arranged in the water bath with thermostatic control Multiple anaerobic fermentation units in pot；

The anaerobic fermentation unit includes that upper and lower side is respectively set the fermentation flask of opening and is arranged on the fermentation flask The exhaust unit of rubber stopper connection at end opening and respectively with the sampling unit that is connect at upper and lower sides end opening；The row Gas unit includes the soft air duct for being inserted into the hard gas-guide tube of the rubber stopper and being connect with the hard gas-guide tube； The other end of the soft airway is deep into the bottom for the graduated cylinder for being inverted in liquid storage device for filling with liquid；The sampling list Member includes the soft conduit being connect with upper and lower sides end opening, the three-way pipe being connect with the soft conduit and is arranged described Soft supravasal hemostatic clamp；One end of the three-way pipe of lower side is connected with liquid getting device for extracting zymotic fluid sample；Upside One end of three-way pipe be connected with the closed soft rubber pipe in one end, collect gaseous sample using gas device is taken.

2. batch anaerobic ferment devices according to claim 1, which is characterized in that be provided in the thermostat water bath Shake babinet；Multiple holes passing through for isothermal liquid is provided on the bottom surface of the concussion babinet；The concussion case The lateral centre position of body is provided with movable plate, is vertically arranged with multiple spacing boards；The movable plate with it is multiple described Spacing board is separated into multiple storage spaces for placing the anaerobic fermentation unit.

3. batch anaerobic ferment devices according to claim 2, which is characterized in that the interposition of the fermentation flask installs It is equipped with the retainer plate of annular；One end of the retainer plate is fixed on the movable plate, and the other end is fixed by spring body On the inner wall of earthquake babinet；The side of the described concussion babinet be equipped with the sliding block interlocked with the movable plate and with it is described The matched slideway of sliding block, the sliding block is connect with motor makes it move back and forth.

4. batch anaerobic ferment devices according to claim 1, which is characterized in that the rubber stopper peripheral wall is equipped with and opens The downward annular baffle of mouth.

5. batch anaerobic ferment devices according to claim 1, which is characterized in that the fermentation flask is 500ml or more The bottle,suction of side opening, the liquid getting device are 5ml disposable syringes, and the gas device that takes is 1ml precision syringes.