CN207659452U - Constant temperature micro anaerobic installation for fermenting - Google Patents

Constant temperature micro anaerobic installation for fermenting Download PDF

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Publication number
CN207659452U
CN207659452U CN201721559128.7U CN201721559128U CN207659452U CN 207659452 U CN207659452 U CN 207659452U CN 201721559128 U CN201721559128 U CN 201721559128U CN 207659452 U CN207659452 U CN 207659452U
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CN
China
Prior art keywords
syringe needle
micro anaerobic
cover
fermenting
constant temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201721559128.7U
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Chinese (zh)
Inventor
高凤芹
杨富裕
孙启忠
德英
陶雅
刘斌
万其号
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Agricultural University
Grassland Research Institute of Chinese Academy of Agricultural Sciences
Original Assignee
China Agricultural University
Grassland Research Institute of Chinese Academy of Agricultural Sciences
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Priority to CN201721559128.7U priority Critical patent/CN207659452U/en
Application granted granted Critical
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Expired - Fee Related legal-status Critical Current
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Abstract

The utility model belongs to use for laboratory chemical apparatuses field, more particularly to a kind of constant temperature micro anaerobic installation for fermenting, including thermostat water bath, be placed in thermostat water bath multiple micro anaerobic fermentation flasks, fill the gas collection bottle of saturated salt solution and the bidirectional needle connector of connection micro anaerobic fermentation flask and gas collection bottle;Bidirectional needle connector includes being arranged in subsurface first syringe needle of saturated salt solution, the second syringe needle of the ullage of zymotic fluid in micro anaerobic fermentation flask is arranged and connecting the gas-guide tube between the first syringe needle and the second syringe needle;The babinet for fixing micro anaerobic fermentation flask is provided in thermostat water bath.The utility model timing or can remove the fermentation flask of certain amount at any time, terminate fermentation, observe index of correlation and the dynamic change of microbial diversity in substrate residue degradation situation and zymotic fluid in real time.

Description

Constant temperature micro anaerobic installation for fermenting
Technical field
The utility model belongs to use for laboratory chemical apparatuses field, ferments and fills more particularly to a kind of constant temperature micro anaerobic It sets.
Background technology
Biomass energy is important regenerative resource, it not only contributes to alleviate fossil energy shortage, but also advantageously reduces Greenhouse gas emission, Devoting Major Efforts To Developing utilize the sustainable development that can be facilitated economic and social.With agricultural crop straw, agricultural grass And the second generation biomass energy that the lignocellulosics such as waste are raw material is widely paid close attention to and in-depth study.With wooden It is exactly producing methane through anaerobic fermentation that cellulose is converted into one of most promising method of biomass energy for material.Anaerobic fermentation produces Biogas be better than a lot of other waste treatment methods, as biomass generate residue it is less, can significantly more efficient removal pathogen, Biogas residue is also used as the fertilizer of plant, and carbon is discharged in the form of methane can also play the role of reduction of greenhouse gas discharge. But it is that material produces bioenergy using lignocellulosics such as stalk, agriculture and forestry organic waste material and draft energy-source plants, development is still In the commercialized primary stage, many key technologies are urgently to be resolved hurrily.It is as complicated in efficiently destroyed ligno-cellulosic materials cell wall Structure reduces crystallinity and the degree of polymerization, promotes lignocellulosic efficient degradation, and it is exactly that we currently urgently solve to improve biogas output Certainly the problem of.Rumen microorganism is natural, the efficient and environmentally friendly conversion system of generally acknowledged lignocellulose degradation material, to anaerobism Fermentation process rumen microorganism diversity and its to the degradation capability of substrate residue deeply systematically research is to crack wooden fibre The plain efficient degradation of dimension, realizes the important research method of its industrialization.
The in vitro method of rumen fluid lignocellulose degradation has many advantages, such as at low cost, efficient, favorable repeatability.But it carries out Import equipment needed for experiment in vitro is expensive, it is difficult to while carrying out the repetition experiment of multiple samples;Domestic relevant device cost It is relatively low, but cannot achieve the real-time collecting to micro-example fermentation substrate and analysis.The identical point of relevant device is both at home and abroad Prodigious advantage is conveniently embodied in analysis zymotic fluid in real time and gas componant detection etc., but can not be completed to substrate residue Real-time collecting and detection.Therefore design it is a can timing detection substrate residue and fermentation broth contents multiple samples constant temperature it is miniature Anaerobic fermentation equipment is extremely necessary.
Utility model content
The purpose of the utility model is to overcome in the prior art the shortcomings that, a kind of constant temperature micro anaerobic fermentation dress is provided It sets.
Technical solution is used by realize the purpose of this utility model:
A kind of constant temperature micro anaerobic installation for fermenting, including thermostat water bath, be placed on it is more in the thermostat water bath A micro anaerobic fermentation flask, the gas collection bottle for filling saturated salt solution and the connection micro anaerobic fermentation flask and described Gas collection bottle bidirectional needle connector;The bidirectional needle connector includes being arranged below saturated common salt water level The first syringe needle, the ullage of zymotic fluid in micro anaerobic fermentation flask is set the second syringe needle and connection it is described first Gas-guide tube between syringe needle and second syringe needle;It is provided in the thermostat water bath and miniature detests for fixed described The babinet of aerobe fermentation bottle;The upper surface of the babinet is provided with multiple holes for across the miniature anaerobic fermentation bottle; Other end faces of the babinet are provided with multiple apertures passing through for warm water.
The micro anaerobic fermentation flask includes body, is made with the integrally formed bottleneck of body, soft rubber Inner cover and rigid plastics made from outer cover;The bottleneck outer wall is equipped with screw thread;The inner cover include upper cover body with And the rubber ring of the ring week fitting bottleneck inner wall;The inner wall of the outer cover is equipped with to be used with the matched lines of the screw thread Outer cover described in screwing and the bottleneck.The outer cover centre position is provided with perforation.
The babinet side is symmetrically arranged with handle.
A plurality of first syringe needle is inserted into the same gas bottle.
It is provided with connector between syringe needle and the gas-guide tube;The connector is crosswise, is vertically arranged with two A siphunculus is interconnected for being inserted into gas-guide tube or syringe needle, described two siphunculus;It is horizontally arranged with into petal-like sheet Grips.
Compared with prior art, the beneficial effects of the utility model are:
1, the utility model selects the setting of micro anaerobic fermentation flask combination thermostat water bath and bidirectional needle connector, no The gas generated inside anaerobism bottle fermentation process can be only excluded, ensures being smoothed out for fermentation test, it may also be ensured that system In stringent anaerobic environment.Meanwhile the design of inner cover and outer cover has been carried out to micro anaerobic fermentation flask, inner cover rubber bodies can Ensure anaerobic environment, and outer cover is not easy to be aoxidized using vulcanie, and can be screwed by screw thread and bottleneck, further ensure Anaerobic environment.
2, the utility model is by this constant temperature micro anaerobic installation for fermenting, timing or can remove certain amount at any time Fermentation flask, terminates fermentation, observes the dynamic of index of correlation and microbial diversity in substrate residue degradation situation and zymotic fluid in real time State changes.The device has device simple, and cost is extremely low, easy to operate, and the test period is short, while can carry out the flat of multiple samples Row experiment reduces the error that different batches test tape comes, and test result is accurate, easy to operate.
Description of the drawings
Fig. 1 is the schematic diagram of the constant temperature micro anaerobic installation for fermenting of the utility model.
Fig. 2 is decomposition and the combining structure schematic diagram of the micro anaerobic fermentation flask of the utility model.
Specific implementation mode
In order to make those skilled in the art more fully understand the technical solution of the utility model, below in conjunction with the accompanying drawings The utility model is described in further detail with most preferred embodiment.
Fig. 1-2 shows a kind of constant temperature micro anaerobic installation for fermenting, including thermostat water bath 1, is placed on the thermostatted water Multiple micro anaerobic fermentation flasks 3 in bath, the gas collection bottle 4 for filling saturated salt solution and the connection micro anaerobic The bidirectional needle connector 5 of fermentation flask and the gas collection bottle;The micro anaerobic fermentation flask used in the present embodiment for 20ml.The bidirectional needle connector includes that subsurface first syringe needle 51 of saturated salt solution, setting is arranged in gas bottle In micro anaerobic fermentation flask the second syringe needle 53 of the ullage of zymotic fluid and connection first syringe needle with it is described Gas-guide tube 54 between second syringe needle;The first syringe needle and the second syringe needle used in the present embodiment is all made of No. 16 syringe needles;It is described Thermostat water bath in be provided with babinet 2 for the micro anaerobic fermentation flask;The upper surface of the babinet is set Multiple holes 21 are equipped with for across the miniature anaerobic fermentation bottle;Other end faces of the babinet are provided with multiple apertures For passing through for warm water.The babinet side is symmetrically arranged with handle and is conveniently drawn off or is put into thermostat water bath.Make To be one such preferred, it is stainless steel, specification that the utility model, which uses the common rack for test tube in laboratory, the rack for test tube, For 21 holes, aperture 30mm.The micro anaerobic fermentation flask include body 31, with the integrally formed bottleneck 32 of body, Outer cover 34 made from inner cover 33 made from soft rubber and rigid plastics;The bottleneck outer wall is equipped with screw thread;Described Inner cover includes the rubber ring 332 of upper cover body 331 and the ring week fitting bottleneck inner wall;The inner wall of the outer cover be equipped with The matched lines of the screw thread is used to screw the outer cover and the bottleneck.The outer cover centre position, which is provided with, wears Hole.Preferably, a plurality of first syringe needle is inserted into the same gas bottle, and sky can be saved while completing effect Between.Connector 52 is provided between syringe needle and the gas-guide tube;The connector is crosswise, and longitudinally disposed there are two logical Pipe is interconnected for being inserted into gas-guide tube or syringe needle, described two siphunculus;Petal-like sheet is horizontally arranged with into hold Part.The gas collection bottle is the wide-mouth bottle of glass material, and specification 500ml fills saturated salt solution in bottle, and saturated concentration is 26.47%.
In short, the utility model selects setting for micro anaerobic fermentation flask combination thermostat water bath and bidirectional needle connector It sets, can not only exclude the gas generated inside anaerobism bottle fermentation process, ensure being smoothed out for fermentation test, it may also be ensured that System is in stringent anaerobic environment.Meanwhile the design of inner cover and outer cover has been carried out to micro anaerobic fermentation flask, inner cover rubber bodies It can ensure anaerobic environment, and outer cover is not easy to be aoxidized using vulcanie, and can be screwed by screw thread and bottleneck, further Ensure anaerobic environment.
The utility model timing or can remove the hair of certain amount at any time by this constant temperature micro anaerobic installation for fermenting Ferment bottle, terminates fermentation, observes index of correlation and the dynamic of microbial diversity in substrate residue degradation situation and zymotic fluid in real time Variation.The device has device simple, and cost is extremely low, easy to operate, and the test period is short, while can carry out the parallel of multiple samples Experiment reduces the error that different batches test tape comes, and test result is accurate, easy to operate.
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and Retouching also should be regarded as the scope of protection of the utility model.

Claims (6)

1. a kind of constant temperature micro anaerobic installation for fermenting, which is characterized in that including thermostat water bath, be placed on the water bath with thermostatic control Multiple micro anaerobic fermentation flasks in pot, the gas collection bottle for filling saturated salt solution and the connection micro anaerobic fermentation The bidirectional needle connector of bottle and the gas collection bottle;The bidirectional needle connector includes that setting is saturated in gas bottle Subsurface first syringe needle of saline solution, the second syringe needle that the ullage of zymotic fluid in micro anaerobic fermentation flask is set and company Connect the gas-guide tube between first syringe needle and second syringe needle;It is provided in the thermostat water bath for fixing The babinet of the micro anaerobic fermentation flask;The upper surface of the babinet is provided with multiple holes for across described miniature Anaerobic fermentation bottle;Other end faces of the babinet are provided with multiple apertures passing through for warm water.
2. constant temperature micro anaerobic installation for fermenting according to claim 1, which is characterized in that the micro anaerobic fermentation flask Including outer cover made from inner cover made from body and the integrally formed bottleneck of body, soft rubber and rigid plastics; The bottleneck outer wall is equipped with screw thread;The inner cover includes the rubber of upper cover body and the ring week fitting bottleneck inner wall Circle;The inner wall of the outer cover is equipped with to be used to screw the outer cover and the bottleneck with the matched lines of screw thread.
3. constant temperature micro anaerobic installation for fermenting according to claim 2, which is characterized in that the outer cover interposition installs It is equipped with perforation.
4. constant temperature micro anaerobic installation for fermenting according to claim 1, which is characterized in that the babinet side is symmetrically set It is equipped with handle.
5. constant temperature micro anaerobic installation for fermenting according to claim 1, which is characterized in that a plurality of first syringe needle is inserted Enter into the same gas bottle.
6. constant temperature micro anaerobic installation for fermenting according to claim 1, which is characterized in that syringe needle and the gas-guide tube it Between be provided with connector;The connector is crosswise, and longitudinally disposed there are two siphunculus to be used to be inserted into gas-guide tube or syringe needle, Two siphunculus are interconnected;It is horizontally arranged with into petal-like sheet grips.
CN201721559128.7U 2017-11-21 2017-11-21 Constant temperature micro anaerobic installation for fermenting Expired - Fee Related CN207659452U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721559128.7U CN207659452U (en) 2017-11-21 2017-11-21 Constant temperature micro anaerobic installation for fermenting

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721559128.7U CN207659452U (en) 2017-11-21 2017-11-21 Constant temperature micro anaerobic installation for fermenting

Publications (1)

Publication Number Publication Date
CN207659452U true CN207659452U (en) 2018-07-27

Family

ID=62945926

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201721559128.7U Expired - Fee Related CN207659452U (en) 2017-11-21 2017-11-21 Constant temperature micro anaerobic installation for fermenting

Country Status (1)

Country Link
CN (1) CN207659452U (en)

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180727

Termination date: 20191121