CN116875423A - Preparation method of galactose yeast-like fermentation product composition - Google Patents
Preparation method of galactose yeast-like fermentation product composition Download PDFInfo
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- CN116875423A CN116875423A CN202310835872.9A CN202310835872A CN116875423A CN 116875423 A CN116875423 A CN 116875423A CN 202310835872 A CN202310835872 A CN 202310835872A CN 116875423 A CN116875423 A CN 116875423A
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- galactose
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- 229930182830 galactose Natural products 0.000 title claims abstract description 47
- 238000000855 fermentation Methods 0.000 title claims abstract description 44
- 230000004151 fermentation Effects 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 239000000203 mixture Substances 0.000 title claims abstract description 14
- 238000011081 inoculation Methods 0.000 claims abstract description 67
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000012360 testing method Methods 0.000 claims description 24
- 238000013016 damping Methods 0.000 claims description 19
- 238000003825 pressing Methods 0.000 claims description 14
- 241000235342 Saccharomycetes Species 0.000 claims description 8
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- 235000015278 beef Nutrition 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000008101 lactose Substances 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 19
- 238000006073 displacement reaction Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
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Abstract
The invention relates to the technical field of galactose yeast fermentation preparation, in particular to a preparation method of a galactose yeast-like fermentation product composition. According to the invention, the automatic scribing inoculation process of the strains can be realized, meanwhile, strain solutions with different proportions can be selected according to actual demands, and the strain solutions are cultivated in different culture dishes by utilizing the rotary table, so that the classified cultivation of the strains is realized, and the subsequent observation and analysis are convenient.
Description
Technical Field
The invention relates to the technical field of galactose yeast fermentation preparation, in particular to a preparation method of a galactose yeast-like fermentation product composition.
Background
The galactose yeast is a haploid of a specific strain of the excellent Saccharomyces cerevisiae fermentation galactose yeast, and haploid separation and DNA fingerprint identification are carried out on the specific strain of the Saccharomyces cerevisiae. The screened excellent haploid galactose specific strain has the advantages of high galactose fermentation speed, high fermentation liquor alcohol content and low reducing sugar content.
The preparation process of the fermentation product of the galactose yeast needs to carry out separation culture on the galactose yeast, the most critical step of the culture process of the galactose yeast is the inoculation process of the strain, the strain inoculation is generally carried out manually at present by adopting a streak inoculation mode, the manual inoculation can be influenced by the personal level and the inoculation habit of operators, the strain inoculation efficiency is low, and the inoculation quality cannot be controlled.
Disclosure of Invention
The invention aims to provide a preparation method of a galactose yeast-like fermentation product composition, and aims to solve the technical problems.
The aim of the invention can be achieved by the following technical scheme:
the preparation method of galactose yeast-like fermentation product composition comprises a inoculation table, wherein a transverse driving pair is arranged at the upper end of the inoculation table, the transverse driving pair is connected with a side plate, a lifting driving pair is arranged on the side wall of the side plate, the lifting driving pair is connected with a lifting seat, a rotating plate is rotatably arranged on the lifting seat, an inoculation assembly is arranged at the bottom of the rotating plate, a conveying assembly is arranged on one side of the upper end of the inoculation table in the length direction, the conveying assembly comprises a supporting table frame and a conveying belt, the supporting table frame is fixedly arranged on the inoculation table, the conveying belt is arranged in the supporting table frame in a sliding manner, strain test tubes are arranged on the conveying belt at equal intervals, a culture assembly is arranged at the upper end of the inoculation table, the culture assembly comprises a rotating table, a plurality of culture dishes are uniformly arranged at the upper end of the rotating table in the circumferential direction, a dish cover is arranged at the upper end of each culture dish, and a cover taking assembly is arranged on one side of the culture dish.
The inoculation subassembly includes upper supporting frame and lower supporting seat, upper supporting frame top and rotor plate rotate to be connected, upper supporting frame bottom and lower supporting seat fixed connection, equidistant running through is provided with a plurality of through-holes in the lower supporting seat, and all slide in every through-hole and be provided with the inoculating loop, inoculating loop top passes lower supporting seat and with clamp plate fixed connection, be provided with reset spring between clamp plate and the lower supporting seat, upper supporting frame bottom is provided with electric telescopic handle, electric telescopic handle's output is connected with the clamp plate, be provided with the guide arm between upper supporting frame and the lower supporting seat, the guide arm slides and runs through the clamp plate, the inside auto-lock subassembly that is used for carrying out locking fixation to the inoculating loop that is provided with of lower supporting seat.
The preparation method comprises the following steps:
step 1: separating strains, namely separating conventional galactose yeasts, separating single galactose yeast strains, preparing strain solutions, and adding the strain solutions into strain test tubes;
step 2: preparing a culture solution, namely heating lactose, peptone, beef extract and water by heating equipment, filtering with gauze and cooling to obtain the culture solution after lactose, peptone and beef extract release internal sugar into water, and adding a culture dish to serve as a galactose saccharomycete culture solution;
step 3: the method comprises the steps of streaking inoculation, rotating an inoculation assembly to the position right above a strain test tube by utilizing a rotating plate, pressing a seed ring downwards to enable the seed ring to extend into the strain test tube and dip strain solution, then lifting the seed ring and rotating the seed ring to the position above a culture dish, and driving the seed ring to perform streaking inoculation in the culture dish by utilizing the rotating plate so as to form galactose saccharomycete colonies in the culture dish;
step 4: and fermenting the galactose yeast, and adding the galactose yeast into a culture dish for fermentation until the fermentation is completed, so as to form a galactose yeast fermentation product.
As a further scheme of the invention: the transverse moving driving pair comprises a transverse guide rail, a transverse moving screw rod and a transverse moving motor, wherein the transverse guide rail is fixedly arranged at the upper end of the inoculation table, the bottom of the side plate is slidably arranged on the transverse guide rail, threads of the transverse moving screw rod penetrate through the bottom of the side plate, and the output end of the transverse moving motor is connected with the transverse moving screw rod.
As a further scheme of the invention: the lifting driving pair comprises a lifting screw rod, a longitudinal guide rail and a lifting motor, wherein the longitudinal guide rail is fixedly arranged on one side of the side plate, the lifting seat is slidably mounted on the longitudinal guide rail, a threaded sleeve is fixedly arranged on one side of the lifting seat, threads of the lifting screw rod penetrate through the threaded sleeve, and the output end of the lifting motor is connected with the lifting screw rod.
As a further scheme of the invention: the lifting seat is characterized in that a supporting plate is fixedly arranged on one side of the lifting seat, a rotating motor is fixedly arranged at the bottom of the supporting plate, and the output end of the rotating motor is connected with the rotating plate.
As a further scheme of the invention: the damping rotary table is characterized in that a damping knob is rotatably mounted at the upper end of the rotary plate, the damping knob is fixedly connected with the top end of the upper supporting frame through a damping rotary shaft, a rotary rod is connected to the damping knob, an arc-shaped chute is formed in the upper end of the rotary plate by taking the damping rotary shaft as the center, a limiting pulley is rotatably mounted at the bottom of the rotary rod, the limiting pulley is slidably mounted in the arc-shaped chute in an adapting mode, an angle scale mark is arranged on the outer edge of the arc-shaped chute, and an indicating needle is arranged at the end of the rotary rod.
As a further scheme of the invention: the self-locking assembly comprises a driving wheel, a connecting column and a clamping block, wherein the driving wheel is rotatably installed in a lower supporting seat, one side of the driving wheel extends out of a corresponding through hole and abuts against an inoculating loop, two ends of the driving wheel are connected with the connecting column through a driving shaft, curved closed ring grooves are formed in the outer wall of the connecting column, the clamping block is symmetrically arranged on two sides of the inoculating loop, a push rod is fixedly connected to the tail of the clamping block, a connecting rod is fixedly connected to one side, close to the connecting column, of the push rod, the end part of the connecting rod is slidably installed in the curved closed ring grooves in an adapting mode, and the driving wheel drives the clamping blocks on two sides to extend out of the through hole through the connecting column and the connecting rod so as to clamp the inoculating loop.
As a further scheme of the invention: the lower supporting seat is internally provided with a plurality of groups of mounting grooves, each group of mounting grooves are communicated with corresponding through holes, the clamping blocks are mounted in the mounting grooves, one side of each mounting groove is communicated with a sliding groove for the push rod to move, and one side of each sliding groove is communicated with a stepping down groove for the connecting rod to move.
As a further scheme of the invention: the cover taking assembly comprises a rotating motor, the rotating motor is fixedly arranged on the inoculation table, a connecting plate is arranged at the output end of the rotating motor, a lifting cylinder is arranged at the upper end of the connecting plate, and a negative pressure sucker is connected with the output end of the lifting cylinder.
The invention has the beneficial effects that:
(1) According to the invention, through arranging the transverse moving driving pair, the lifting driving pair, the rotating plate, the inoculation assembly, the conveying assembly and the culture assembly, the automatic scribing inoculation process of strains can be realized, meanwhile, strain solutions with different proportions can be selected according to actual requirements, and the strain solutions are cultured into different culture dishes by utilizing the rotating table, so that the classified culture of the strains is realized, and the subsequent observation and analysis are convenient.
(2) According to the invention, the self-locking assembly is arranged, the inoculating loop drives the driving wheel to rotate during scribing inoculation, the driving rod wheel drives the clamping blocks at two sides to move in opposite directions through the transmission cooperation of the connecting column and the connecting rod until the clamping blocks are abutted against the side wall of the inoculating loop to realize locking and fixing of the inoculating loop, so that the descending process of the inoculating loop is limited to avoid excessive displacement, and meanwhile, the clamping and locking effect of the inoculating loop keeps stable all the time during scribing inoculation, so that shaking cannot occur easily, and the accuracy of the scribing position and the scribing inoculation effect are improved.
Drawings
The invention is further described below with reference to the accompanying drawings.
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic structural view of the middle lifting seat and the rotating plate of the present invention.
FIG. 3 is a schematic view of the structure of the inoculating module of the present invention.
Fig. 4 is a schematic cross-sectional view in the direction A-A of fig. 3.
Fig. 5 is a schematic cross-sectional view in the direction B-B of fig. 4.
FIG. 6 is a schematic view of the structure of the self-locking assembly of the present invention.
Fig. 7 is a schematic view of the structure of the cap taking assembly in the present invention.
In the figure: 1. an inoculation table; 2. a side plate; 3. a sideslip driving pair; 31. a transverse guide rail; 32. a traversing screw; 33. a traversing motor; 4. a lifting driving pair; 41. lifting screw rods; 42. a longitudinal guide rail; 43. a lifting motor; 5. a lifting seat; 51. a screw sleeve; 52. a support plate; 53. a rotating electric machine; 6. a rotating plate; 601. an arc chute; 61. a damping knob; 62. a rotating rod; 621. a limit pulley; 63. an indicator needle; 64. angle graduation marks; 7. an inoculation assembly; 71. an upper support frame; 72. a lower support base; 7201. a mounting groove; 7202. a chute; 7203. a relief groove; 721. a driving wheel; 7211. a drive shaft; 722. a connecting column; 7221. curved closed ring groove; 723. a clamping block; 7231. a push rod; 7232. a connecting rod; 73. an inoculating loop; 74. a pressing plate; 75. an electric telescopic rod; 76. a return spring; 77. a guide rod; 8. a transfer assembly; 81. a support stand; 82. a conveyor belt; 83. a strain test tube; 9. a culturing assembly; 91. a rotary table; 92. a culture dish; 93. a dish cover; 10. taking a cover assembly; 101. a rotary motor; 102. a connecting plate; 103. a lifting cylinder; 104. a negative pressure sucking disc.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1, the device adopted in the preparation method comprises an inoculation table 1, wherein a traversing driving pair 3 is arranged at the upper end of the inoculation table 1, the traversing driving pair 3 is connected with a side plate 2, a lifting driving pair 4 is arranged on the side wall of the side plate 2, the lifting driving pair 4 is connected with a lifting seat 5, a rotating plate 6 is rotatably arranged on the lifting seat 5, an inoculation assembly 7 is arranged at the bottom of the rotating plate 6, a conveying assembly 8 is arranged on one side of the upper end of the inoculation table 1 along the length direction, the conveying assembly 8 comprises a support table 81 and a conveying belt 82, the support table 81 is fixedly arranged on the inoculation table 1, the conveying belt 82 is slidingly arranged in the support table 81, strain test tubes 83 are arranged on the conveying belt 82 at equal intervals, a culture assembly 9 is arranged at the upper end of the inoculation table 1, the culture assembly 9 comprises a rotary table 91, a plurality of culture dishes 92 are uniformly arranged at the periphery of the upper end of the rotary table 91, a dish cover 93 is arranged at the upper end of the culture dish 92, and a cover taking assembly 10 is arranged at one side of the culture dish 93.
As shown in fig. 2 and 3, the inoculation assembly 7 comprises an upper supporting frame 71 and a lower supporting seat 72, the top end of the upper supporting frame 71 is rotationally connected with the rotating plate 6, the bottom end of the upper supporting frame 71 is fixedly connected with the lower supporting seat 72, a plurality of through holes are formed in the lower supporting seat 72 at equal intervals in a penetrating manner, an inoculation ring 73 is slidably arranged in each through hole, the top end of the inoculation ring 73 penetrates through the lower supporting seat 72 and is fixedly connected with a pressing plate 74, a reset spring 76 is arranged between the pressing plate 74 and the lower supporting seat 72, an electric telescopic rod 75 is arranged at the bottom of the upper supporting frame 71, the output end of the electric telescopic rod 75 is connected with the pressing plate 74, a guide rod 77 is arranged between the upper supporting frame 71 and the lower supporting seat 72, the guide rod 77 slidably penetrates through the pressing plate 74, and a self-locking assembly for locking and fixing the inoculation ring 73 is arranged in the lower supporting seat 72.
Specifically, in the inoculation process, the strain test tube 83 containing strain solution is conveyed along with the conveyor belt 82, the lateral moving driving pair 3 is utilized to drive the side plate 2 to carry out linear displacement, meanwhile, the rotating plate 6 is utilized to rotate and drive the inoculating assembly 7 to move to the position right above the designated strain test tube 83, then the lifting driving pair 4 is utilized to reduce the overall height of the inoculating assembly 7, then the electric telescopic rod 75 is utilized to downwards press the pressing plate 74, thereby driving the inoculating loop 73 to further downwards displace, so as to finely control the descending height of the inoculating loop 73, enable the inoculating loop 73 to extend into the strain test tube 83 and dip in strain solution, then the inoculating loop 73 upwards lifts, the rotating plate 6 and the lateral moving driving pair 3 are utilized to enable the inoculating loop 73 to move to the position right above the culture dish 92, the cover 93 above the culture dish 92 is utilized to be taken out by utilizing the cover taking assembly 10, the rotating plate 6 is utilized to drive the inoculating loop 73 to carry out streak inoculation in the culture dish 92, multiple times of streak inoculation can be realized by utilizing the plurality of inoculating loops 73 to repeatedly operate, simultaneously, strains with different proportions can be selected according to actual demands, and the rotating table 91 is utilized to be further downwards to cultivate strains into different culture dishes 92, then the strains with the rotating table 73, the inoculating loop is automatically controlled, the strain is enabled to extend into the strain 83, then to extend into the strain test tube, and then be conveniently observed, the strain is conveniently and the strain is conveniently observed, the strain is conveniently and the strain is sampled, and the strain is manually, and the strain is used to be sampled.
The preparation method comprises the following steps:
step 1: separating strains, namely separating conventional galactose yeasts, separating single galactose yeast strains, preparing strain solutions, and adding the strain solutions into strain test tubes 83;
step 2: preparing a culture solution, namely heating lactose, peptone, beef extract and water by heating equipment, filtering with gauze and cooling to obtain the culture solution after lactose, peptone and beef extract release internal sugar into water, and adding a culture dish 92 as a galactose saccharomycete culture solution;
step 3: the inoculation assembly 7 is rotated to be right above the strain test tube 83 by using the rotating plate 6, the seed ring 73 is pressed down to extend into the strain test tube 83 and dip strain solution, then the seed ring 73 is lifted and rotated to be above the culture dish 92, and the rotating plate 6 is used for driving the seed ring 73 to perform the inoculation of streaking in the culture dish 92 so as to form galactose yeast colonies in the culture dish 92;
step 4: and fermenting the galactose yeast, and adding the galactose yeast into a culture dish 92 for fermentation until the fermentation is completed, so as to form a galactose yeast fermentation product.
As shown in fig. 1, the traversing driving pair 3 comprises a traversing guide rail 31, a traversing screw rod 32 and a traversing motor 33, the traversing guide rail 31 is fixedly arranged at the upper end of the inoculating platform 1, the bottom of the side plate 2 is slidably arranged on the traversing guide rail 31, the traversing screw rod 32 is threaded through the bottom of the side plate 2, and the output end of the traversing motor 33 is connected with the traversing screw rod 32.
Further, the lifting driving pair 4 comprises a lifting screw 41, a longitudinal guide rail 42 and a lifting motor 43, the longitudinal guide rail 42 is fixedly arranged on one side of the side plate 2, the lifting seat 5 is slidably mounted on the longitudinal guide rail 42, a threaded sleeve 51 is fixedly arranged on one side of the lifting seat 5, threads of the lifting screw 41 penetrate through the threaded sleeve 51, and the output end of the lifting motor 43 is connected with the lifting screw 41.
Further, a supporting plate 52 is fixedly arranged on one side of the lifting seat 5, a rotating motor 53 is fixedly arranged at the bottom of the supporting plate 52, and an output end of the rotating motor 53 is connected with the rotating plate 6.
Specifically, the traversing motor 33 drives the traversing screw 32 to rotate, and the traversing screw 32 drives the side plate 2 to linearly move along the transverse guide rail 31, so that the horizontal position of the inoculation assembly 7 can be adjusted, the conveyor belt 82 is matched to drive the movement process of the strain test tube 83, and the inoculating loop 73 can be conveniently dipped with strain solutions in different strain test tubes 83. The lifting motor 43 drives the lifting screw 41 to rotate, and the lifting screw 41 drives the lifting seat 5 to slide up and down along the longitudinal guide rail 42 through the threaded sleeve 51, so that the overall height of the inoculation assembly 7 can be adjusted, and the inoculation ring 73 is convenient to approach the strain test tube 83 or the culture dish 92.
As shown in fig. 2, a damping knob 61 is rotatably mounted at the upper end of the rotating plate 6, the damping knob 61 is fixedly connected with the top end of the upper supporting frame 71 through a damping rotating shaft (not shown in the drawing), a rotating rod 62 is connected to the damping knob 61, an arc-shaped chute 601 is arranged at the upper end of the rotating plate 6 by taking the damping rotating shaft as the center, a limiting pulley 621 is rotatably mounted at the bottom of the rotating rod 62, the limiting pulley 621 is adapted to be slidably mounted in the arc-shaped chute 601, an angle scale mark 64 is arranged along the outer edge of the arc-shaped chute 601, and an indicator 63 is arranged at the end of the rotating rod 62.
Specifically, in the scribing inoculation process, when the scribing angle of the inoculating loop 73 needs to be adjusted, the damping knob 61 is rotated to drive the upper supporting frame 71 to rotate, so that the inoculating loop 73 below is driven to deflect, in the embodiment, the upper supporting frame 71, the rotating rod 62 and the indicating needle 63 are kept parallel in the same vertical plane, when the damping knob 61 rotates, the rotating rod 62 and the indicating needle 63 synchronously rotate, meanwhile, the limiting pulley 621 always keeps sliding in the arc-shaped chute 601, so that the balance stability of the rotation process is ensured, the adjustment angle can be displayed very intuitively according to the mark indication of the indicating needle 63 on the angle scale mark 64, and the scribing angle of the inoculating loop 73 can be conveniently and rapidly adjusted.
As shown in fig. 4-6, the self-locking assembly comprises a driving wheel 721, a connecting column 722 and a clamping block 723, the driving wheel 721 is rotatably installed in the lower supporting seat 72, one side of the driving wheel 721 extends out of a corresponding through hole and abuts against the inoculating loop 73, two ends of the driving wheel 721 are connected with the connecting column 722 through driving shafts 7211, curved closed ring grooves 7221 are formed in the outer wall of the connecting column 722, the clamping block 723 is symmetrically arranged on two sides of the inoculating loop 73, the tail of the clamping block 723 is fixedly connected with a push rod 7231, one side of the push rod 7231, close to the connecting column 722, is fixedly connected with a connecting rod 7232, the end part of the connecting rod 7232 is slidably installed in the curved closed ring groove 7221 in an adapting mode, and the driving wheel 721 drives the clamping blocks 723 on two sides to extend out of the through holes through the connecting column 722 and the connecting rod 7232 so as to clamp the inoculating loop 73.
As shown in fig. 5, a plurality of groups of mounting grooves 7201 are provided in the lower support base 72, each group of mounting grooves 7201 is communicated with a corresponding through hole, the clamping block 723 is mounted in the mounting groove 7201, a sliding groove 7202 for the movement of the push rod 7231 is provided on one side of the mounting groove 7201 in a communicating manner, and a yielding groove 7203 for the movement of the connecting rod 7232 is provided on one side of the sliding groove 7202 in a communicating manner.
Specifically, in the scribing inoculation process, the electric telescopic rod 75 presses the pressing plate 74 downwards, so that the pressing plate 74 pushes the inoculating loop 73 to move downwards, at this time, the reset spring 76 is extruded to deform and compress, meanwhile, in the downward moving process of the inoculating loop 73, the side wall of the reset spring is in friction rolling with the driving wheel 721, thereby driving the driving wheel 721 to rotate, the driving wheel 721 drives the connecting columns 722 on two sides to rotate simultaneously through the driving shafts 7211 on two ends, the connecting columns 722 are in rotation, the connecting columns 722 can drive the push rod 7231 to perform linear displacement by utilizing the matching of the curved closed annular groove 7221 and the connecting rods 7232, the directions of the curved closed annular groove 7221 on the connecting columns 722 on two ends in the embodiment are opposite, so that the push rod 7231 always keeps moving in opposite directions, and the push rods 7231 on two sides simultaneously push the clamping blocks 723 are close to each other until the clamping blocks 723 abut against the side wall of the inoculating loop 73, so that the locking and fixing of the inoculating loop 73 are realized, the descending process of the inoculating loop 73 is limited to avoid excessive displacement, and meanwhile, the clamping and locking effect on the inoculating loop 73 is kept stable in scribing, so that the scribing is in the scribing process, the scribing position is not easy, so that the scribing position is improved, and scribing accuracy is achieved.
As shown in fig. 7, the cap taking assembly 10 comprises a rotary motor 101, the rotary motor 101 is fixedly arranged on the inoculating table 1, a connecting plate 102 is arranged at the output end of the rotary motor 101, a lifting cylinder 103 is arranged at the upper end of the connecting plate 102, and a negative pressure sucker 104 is connected at the output end of the lifting cylinder 103.
Specifically, before the accurate streaking inoculation, the rotary table 91 will rotate at a constant speed until the culture dish 92 appointed by the upper end rotates to the streaking inoculation station, at this time, the rotary motor 101 drives the connecting plate 102 to rotate, so that the negative pressure suction cup 104 is located right above the culture dish 92, then the lifting cylinder 103 drives the negative pressure suction cup 104 to move downwards until the negative pressure suction cup 104 is attached to the dish cover 93 of the culture dish 92, at this time, the negative pressure suction cup 104 generates negative pressure suction force, and at the same time, the rotary return is raised, when the dish cover 93 is required to be covered, the reverse operation is performed, thereby realizing automatic opening and closing of the dish cover 93, and being beneficial to improving the streaking inoculation efficiency.
The working principle of the invention is as follows: in the preparation process, firstly, the strain is separated, the conventional galactose saccharomycete is taken for separation, the single galactose saccharomycete strain is separated, a strain solution is prepared and added into a strain test tube 83, then various nutrient substances are used for preparing a culture solution and adding the culture solution into a culture dish 92, and then the preparation for streaking inoculation is started, in the inoculation process, the strain test tube 83 containing the strain solution is conveyed along with a conveying belt 82, a lateral moving driving pair 3 is utilized to drive a side plate 2 to carry out linear displacement, meanwhile, a rotating plate 6 rotates to drive an inoculating assembly 7 to move to the position right above the designated strain test tube 83, then the lifting driving pair 4 is utilized to reduce the integral height of the inoculating assembly 7, then an electric telescopic rod 75 is utilized to downwards press a pressing plate 74, so that an inoculating loop 73 is driven to further downwards displace to finely control the descending height of the inoculating loop 73, the inoculating loop 73 is enabled to extend into the strain test tube 83, then the inoculating loop 73 is lifted upwards, the inoculating loop 73 is enabled to move right above the culture dish 92 by utilizing the lateral moving pair 3, a cover plate 93 is driven by utilizing the lateral moving pair 3 to take out the lateral moving pair 2 in the linear displacement, meanwhile, the rotating plate 6 drives the inoculating loop 3 to move the inoculating assembly 7 to the designated galactose saccharomycete 83 to carry out the fermentation product in the rotating plate 92, the fermentation product is repeatedly, the fermentation galactosis saccharomycete is formed in the rotating plate 92, and the fermentation product is inoculated in the fermentation culture dish is formed, and the fermentation product is repeatedly used to be used for the fermentation, and the strain and the fermentation product is subjected to and the fermentation, and the fermentation product is subjected to the fermentation, and the fermentation product is repeatedly used to and the fermentation, and after the fermentation is subjected to and the fermentation.
The foregoing describes one embodiment of the present invention in detail, but the description is only a preferred embodiment of the present invention and should not be construed as limiting the scope of the invention. All equivalent changes and modifications within the scope of the present invention are intended to be covered by the present invention.
Claims (8)
1. The preparation method of the galactose yeast-like fermentation product composition is characterized in that a device adopted by the preparation method comprises an inoculation table (1), wherein the upper end of the inoculation table (1) is provided with a transverse driving pair (3), the transverse driving pair (3) is connected with a side plate (2), a lifting driving pair (4) is arranged on the side wall of the side plate (2), the lifting driving pair (4) is connected with a lifting seat (5), a rotating plate (6) is rotatably arranged on the lifting seat (5), an inoculation assembly (7) is arranged at the bottom of the rotating plate (6), one side of the upper end of the inoculation table (1) is provided with a conveying assembly (8) along the length direction, the conveying assembly (8) comprises a supporting table (81) and a conveying belt (82), the supporting table (81) is fixedly arranged on the inoculation table (1), the conveying belt (82) is slidably arranged in the supporting table (81), a strain test tube (83) is arranged on the conveying belt (82) at equal intervals, a culture assembly (9) is arranged at the upper end of the inoculation table (1), a plurality of culture dishes (91) are uniformly arranged at the upper ends of the rotating table (92), a cover taking assembly (10) is arranged on one side of the rotary table (91);
the inoculation assembly (7) comprises an upper supporting frame (71) and a lower supporting seat (72), the top end of the upper supporting frame (71) is rotationally connected with a rotating plate (6), the bottom end of the upper supporting frame (71) is fixedly connected with the lower supporting seat (72), a plurality of through holes are formed in the lower supporting seat (72) at equal intervals in a penetrating mode, an inoculation ring (73) is slidably arranged in each through hole, the top end of the inoculation ring (73) penetrates through the lower supporting seat (72) and is fixedly connected with a pressing plate (74), a reset spring (76) is arranged between the pressing plate (74) and the lower supporting seat (72), an electric telescopic rod (75) is arranged at the bottom of the upper supporting frame (71), a guide rod (77) is arranged between the upper supporting frame (71) and the lower supporting seat (72), the guide rod (77) is slidably penetrated through the pressing plate (74), and a self-locking assembly used for locking and fixing the inoculation ring (73) is arranged in the lower supporting seat (72).
The preparation method comprises the following steps:
step 1: separating strains, namely separating conventional galactose yeasts, separating single galactose yeast strains, preparing strain solutions, and adding the strain solutions into strain test tubes (83);
step 2: preparing a culture solution, namely heating lactose, peptone, beef extract and water by heating equipment, filtering with gauze and cooling to obtain the culture solution after lactose, peptone and beef extract release internal sugar into water, and adding a culture dish (92) as a galactose saccharomycete culture solution;
step 3: the method comprises the steps of streaking inoculation, rotating an inoculation assembly (7) to be right above a strain test tube (83) by utilizing a rotating plate (6), pressing a seed ring (73) downwards to enable the seed ring to extend into the strain test tube (83) and dip strain solution, then lifting the seed ring (73) and rotating the seed ring to be above a culture dish (92), and driving the seed ring (73) to carry out streaking inoculation in the culture dish (92) by utilizing the rotating plate (6) so as to form galactose yeast colonies in the culture dish (92);
step 4: and fermenting the galactose yeast, and adding the galactose yeast into a culture dish (92) for fermentation until the fermentation is completed, so as to form a galactose yeast fermentation product.
2. The preparation method of the galactose yeast-like fermentation product composition according to claim 1, wherein the traverse driving pair (3) comprises a transverse guide rail (31), a traverse screw (32) and a traverse motor (33), the transverse guide rail (31) is fixedly arranged at the upper end of the inoculation table (1), the bottom of the side plate (2) is slidably arranged on the transverse guide rail (31), the traverse screw (32) penetrates through the bottom of the side plate (2) in a threaded manner, and the output end of the traverse motor (33) is connected with the traverse screw (32).
3. The preparation method of the galactose yeast-like fermentation product composition according to claim 1, wherein the lifting driving pair (4) comprises a lifting screw (41), a longitudinal guide rail (42) and a lifting motor (43), the longitudinal guide rail (42) is fixedly arranged on one side of the side plate (2), the lifting seat (5) is slidably mounted on the longitudinal guide rail (42), a screw sleeve (51) is fixedly arranged on one side of the lifting seat (5), threads of the lifting screw (41) penetrate through the screw sleeve (51), and the output end of the lifting motor (43) is connected with the lifting screw (41).
4. The preparation method of the galactose yeast-like fermentation product composition according to claim 1, wherein a supporting plate (52) is fixedly arranged on one side of the lifting seat (5), a rotating motor (53) is fixedly arranged at the bottom of the supporting plate (52), and the output end of the rotating motor (53) is connected with the rotating plate (6).
5. The preparation method of the galactose yeast like fermentation product composition according to claim 4, wherein a damping knob (61) is rotatably mounted at the upper end of the rotating plate (6), the damping knob (61) is fixedly connected with the top end of the upper supporting frame (71) through a damping rotating shaft, a rotating rod (62) is connected to the damping knob (61), an arc-shaped chute (601) is arranged at the upper end of the rotating plate (6) by taking the damping rotating shaft as a center, a limiting pulley (621) is rotatably mounted at the bottom of the rotating rod (62), the limiting pulley (621) is slidably mounted in the arc-shaped chute (601) in a matching manner, an angle scale mark (64) is arranged along the outer edge of the arc-shaped chute (601), and an indicating needle (63) is arranged at the end of the rotating rod (62).
6. The preparation method of the galactose yeast-like fermentation product composition according to claim 1, wherein the self-locking assembly comprises a driving wheel (721), a connecting column (722) and a clamping block (723), the driving wheel (721) is rotatably installed in a lower supporting seat (72), one side of the driving wheel (721) extends out of a corresponding through hole and abuts against an inoculating ring (73), two ends of the driving wheel (721) are connected with the connecting column (722) through driving shafts (7211), curved closed ring grooves (7221) are formed in the outer wall of the connecting column (722), the clamping blocks (723) are symmetrically arranged on two sides of the inoculating ring (73), pushing rods (7231) are fixedly connected to the tail portions of the clamping blocks (723), one side, close to the connecting column (722), of the pushing rods (7231) is fixedly connected with connecting rods (7232), the end portions of the connecting rods (7232) are adaptively and slidably installed in the curved closed ring grooves (7221), and the driving wheel (721) drives the clamping blocks (723) on two sides to extend out of the inoculating ring (73) through the connecting column (722).
7. The preparation method of the galactose yeast-like fermentation product composition according to claim 6, wherein a plurality of groups of mounting grooves (7201) are formed in the lower supporting seat (72), each group of mounting grooves (7201) is communicated with a corresponding through hole, the clamping block (723) is mounted in the mounting groove (7201), a chute (7202) for a push rod (7231) to move is formed in one side of the mounting groove (7201) in a communicating manner, and a yielding groove (7203) for a connecting rod (7232) to move is formed in one side of the chute (7202) in a communicating manner.
8. The preparation method of the galactose yeast like fermentation product composition according to claim 1, wherein the cover taking assembly (10) comprises a rotating motor (101), the rotating motor (101) is fixedly arranged on the inoculation table (1), a connecting plate (102) is arranged at the output end of the rotating motor (101), a lifting cylinder (103) is arranged at the upper end of the connecting plate (102), and a negative pressure sucker (104) is connected to the output end of the lifting cylinder (103).
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Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002098704A (en) * | 2000-09-25 | 2002-04-05 | Olympus Optical Co Ltd | Autoanalyzer of bacteria |
| US6617146B1 (en) * | 1997-03-17 | 2003-09-09 | Canadian Space Agency | Method and apparatus for automatically inoculating culture media with bacterial specimens from specimen containers |
| JP2004073486A (en) * | 2002-08-19 | 2004-03-11 | Shimizu Kikaku:Kk | Gripping arm device for crane game machine |
| CN1918280A (en) * | 2004-01-22 | 2007-02-21 | 默德威特科技有限公司 | Microbial streaking device |
| US20150024425A1 (en) * | 2013-07-22 | 2015-01-22 | Board Of Regents, The University Of Texas System | Apparatuses And Methods For Forming Wounds In Cell Layers |
| CN204550576U (en) * | 2015-04-15 | 2015-08-12 | 重庆道鑫生物科技有限公司 | A kind of system for automatic marker making mechanism |
| CN204981874U (en) * | 2015-09-22 | 2016-01-20 | 重庆道鑫生物科技有限公司 | Marking off mechanism |
| CN205387572U (en) * | 2016-03-18 | 2016-07-20 | 王怡娜 | Bacterium separation inoculation machine based on triaxial removes |
| CN107118950A (en) * | 2017-05-06 | 2017-09-01 | 尹康康 | A kind of bacterium automatic vaccination instrument |
| CN109294897A (en) * | 2018-10-31 | 2019-02-01 | 威海祥和迎春医疗科技有限公司 | Micro-biological samples pre-treatment intelligent robot |
| CN209702757U (en) * | 2019-03-26 | 2019-11-29 | 宜昌市万泽生物科技有限公司 | A kind of plate streaking selects the automatic device of purebred bacterium colony |
| CN110672824A (en) * | 2019-10-30 | 2020-01-10 | 深圳市优瑞特检测技术有限公司 | Testing system and testing method for degradability of polyethylene packaging material |
| CN211595640U (en) * | 2019-08-16 | 2020-09-29 | 济南百博生物技术股份有限公司 | High-efficient little biological sample pretreatment system |
| CN212932188U (en) * | 2020-08-15 | 2021-04-09 | 湖北周黑鸭食品工业园有限公司 | Clamp for testing tensile force of food packaging bag and tensile force testing machine |
| CN213295346U (en) * | 2020-09-24 | 2021-05-28 | 济南朗迈生物科技有限公司 | Sample fungus taking and scribing rotating mechanism |
| CN213739459U (en) * | 2020-10-13 | 2021-07-20 | 上海莫杜生物科技有限公司 | A subregion marking device for culture dish |
| CN115109687A (en) * | 2022-05-23 | 2022-09-27 | 商丘职业技术学院 | Microbial community inoculation device |
| CN115109689A (en) * | 2022-08-01 | 2022-09-27 | 安徽医学高等专科学校 | Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument |
| CN218910320U (en) * | 2023-02-21 | 2023-04-25 | 朗迈(山东)生物科技有限公司 | Multifunctional linkage device for culture dish |
-
2023
- 2023-07-09 CN CN202310835872.9A patent/CN116875423B/en active Active
Patent Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6617146B1 (en) * | 1997-03-17 | 2003-09-09 | Canadian Space Agency | Method and apparatus for automatically inoculating culture media with bacterial specimens from specimen containers |
| JP2002098704A (en) * | 2000-09-25 | 2002-04-05 | Olympus Optical Co Ltd | Autoanalyzer of bacteria |
| JP2004073486A (en) * | 2002-08-19 | 2004-03-11 | Shimizu Kikaku:Kk | Gripping arm device for crane game machine |
| CN1918280A (en) * | 2004-01-22 | 2007-02-21 | 默德威特科技有限公司 | Microbial streaking device |
| US20150024425A1 (en) * | 2013-07-22 | 2015-01-22 | Board Of Regents, The University Of Texas System | Apparatuses And Methods For Forming Wounds In Cell Layers |
| CN204550576U (en) * | 2015-04-15 | 2015-08-12 | 重庆道鑫生物科技有限公司 | A kind of system for automatic marker making mechanism |
| CN204981874U (en) * | 2015-09-22 | 2016-01-20 | 重庆道鑫生物科技有限公司 | Marking off mechanism |
| CN205387572U (en) * | 2016-03-18 | 2016-07-20 | 王怡娜 | Bacterium separation inoculation machine based on triaxial removes |
| CN107118950A (en) * | 2017-05-06 | 2017-09-01 | 尹康康 | A kind of bacterium automatic vaccination instrument |
| CN109294897A (en) * | 2018-10-31 | 2019-02-01 | 威海祥和迎春医疗科技有限公司 | Micro-biological samples pre-treatment intelligent robot |
| CN209702757U (en) * | 2019-03-26 | 2019-11-29 | 宜昌市万泽生物科技有限公司 | A kind of plate streaking selects the automatic device of purebred bacterium colony |
| CN211595640U (en) * | 2019-08-16 | 2020-09-29 | 济南百博生物技术股份有限公司 | High-efficient little biological sample pretreatment system |
| CN110672824A (en) * | 2019-10-30 | 2020-01-10 | 深圳市优瑞特检测技术有限公司 | Testing system and testing method for degradability of polyethylene packaging material |
| CN212932188U (en) * | 2020-08-15 | 2021-04-09 | 湖北周黑鸭食品工业园有限公司 | Clamp for testing tensile force of food packaging bag and tensile force testing machine |
| CN213295346U (en) * | 2020-09-24 | 2021-05-28 | 济南朗迈生物科技有限公司 | Sample fungus taking and scribing rotating mechanism |
| CN213739459U (en) * | 2020-10-13 | 2021-07-20 | 上海莫杜生物科技有限公司 | A subregion marking device for culture dish |
| CN115109687A (en) * | 2022-05-23 | 2022-09-27 | 商丘职业技术学院 | Microbial community inoculation device |
| CN115109689A (en) * | 2022-08-01 | 2022-09-27 | 安徽医学高等专科学校 | Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument |
| CN218910320U (en) * | 2023-02-21 | 2023-04-25 | 朗迈(山东)生物科技有限公司 | Multifunctional linkage device for culture dish |
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