CN111323285B - Automatic change dyeing decoloration device - Google Patents

Automatic change dyeing decoloration device Download PDF

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Publication number
CN111323285B
CN111323285B CN202010165865.9A CN202010165865A CN111323285B CN 111323285 B CN111323285 B CN 111323285B CN 202010165865 A CN202010165865 A CN 202010165865A CN 111323285 B CN111323285 B CN 111323285B
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box
dyeing
ring
liquid
adapter
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CN111323285A (en
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尹铁球
彭鹏
任碧琼
杨雪娇
吴超利
杨欢
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Guilin Medical University
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Guilin Medical University
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Fiber Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

An automatic dyeing and decoloring device comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a supporting leg, an upper cover, a liquid inlet, a supporting plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a supporting piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle; the main part is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, and four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box.

Description

Automatic change dyeing decoloration device
Technical Field
The invention relates to the field of medical equipment, in particular to an automatic dyeing and decoloring device.
Background
The polyacrylamide gel electrophoresis can separate protein into a plurality of zones according to different mobilities generated by the difference of charges of different biological macromolecules such as protein molecules and the difference of molecular sizes, and if the separated and purified sample only contains the same protein, only one zone should be separated after the protein sample is electrophoresed. After electrophoresis, the separated zones need to be stained with a dye for observation, the commonly used dyes are silver nitrate, coomassie Brilliant blue R250 or G250, and other areas of the gel without the sample are also stained during staining, and are easily eluted by an organic solvent, so that the gel needs to be decolorized by a decolorizing solution.
The general dyeing and decoloring process comprises the following steps: placing the gel after electrophoresis in a box, adding a reagent for fixing for a certain time, and pouring out the reagent in the box; washing for a certain time, and pouring out the reagent in the box; dyeing for a certain time, and pouring out the reagent in the box; then repeatedly eluting several times, pouring out the reagent in the box, taking out the gel and storing. When dyeing and decoloring, the box is always placed on a shaking table or an oscillator, on one hand, reagents are uniformly mixed, and on the other hand, the shaking can ensure that the dyeing and decoloring effects are better.
However, in practical use, the following problems exist:
1. dyeing and decoloring devices in the prior art need long-term uninterrupted dyeing liquid or decoloring liquid replacement in dyeing and decoloring processes, liquid is easily scattered outside a recovery bottle to cause pollution, and gel is easily broken due to repeated moving in the liquid replacement process. And the gel, the dyeing solution and the decoloring solution contain a large amount of toxic and harmful substances, and the harmful substances or harmful gases (such as formaldehyde commonly used in the developing solution) are contacted to volatilize and cause harm to human bodies, thereby influencing the body health of operators.
2. The dyeing and decoloring device in the prior art has many technical difficulties which are not overcome, and the cost is very high due to the fact that a large number of remote control type electromagnetic valves such as a plurality of liquid inlet remote control valves and a plurality of liquid outlet remote control valves are used.
3. The dyeing and decoloring device in the prior art needs a lot of pipelines due to frequent and large flow path circulation, and only considers the functionality, such as designing a complex pipeline structure in the device for circulating various dyeing and decoloring liquids, so that the manufacturing cost is very high, or the inside is communicated through a hose so that the installation is very difficult.
4. In the dyeing and decoloring device in the prior art, a shaking table is needed in one process, however, the shaking table and the dyeing and decoloring device are difficult to integrate together, even if some inventions integrate the shaking table together, only one wish or assumption cannot be realized at all, and when the shaking table shakes, the shaking table drives the dyeing box and a large number of connected pipelines to shake together, which is not a practical and reliable technical scheme at all.
5. The dyeing and decoloring device in the prior art has many problems caused by integration, and firstly, when a shaking table is used, a dyeing box has insufficient space for shaking.
6. The dyeing and decoloring device in the prior art only considers the discharge of liquid and does not consider the dispersion of waste gas, and the concentrated waste gas influences the health of operators when being manually started.
7. The dyeing and decoloring device in the prior art has no effective flowing power structure when liquid flows, so that the liquid does not flow smoothly.
Disclosure of Invention
In order to overcome the above problems, the present invention proposes a solution to solve the above problems simultaneously.
The technical scheme adopted by the invention for solving the technical problems is as follows: an automatic dyeing and decoloring device comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a supporting leg, an upper cover, a liquid inlet, a supporting plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a supporting piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle;
the main body is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box, the bottom surfaces of the four boxes do not completely cover the supporting plate, the supporting plate is also provided with the adapter ring, the four boxes surround the adapter ring, the inner sides of the four boxes are respectively provided with a liquid inlet, the outer wall of the adapter ring is provided with an opening, and the opening is sequentially communicated with the four liquid inlets through the rotation of the adapter ring; a first handle is arranged above the adapter ring, the through-flow cover is connected to the inner side of the adapter ring, the inner side of the adapter ring is in fluid communication with the inner side of the through-flow cover, an upright post is arranged below the through-flow cover, a support leg is arranged below the upright post, the height of the upper surface of the edge of the through-flow cover is the same as that of the upper surface of the support leg, the support leg is supported on the upper cover, and the guide plate is arranged below the upper hole of the upper cover;
the gas diffusion box is arranged below the supporting plate, surrounds the dyeing bin, is provided with the vent hole on the inner side and is internally provided with the dyeing box; the control valve is arranged below the dyeing box and controls the fluid communication between the inside and the outside of the dyeing box; the dyeing box is supported on the rocking bed, a convex shoulder and a groove are arranged on the upper surface of the rocking bed, and liquid flows into the groove through the convex shoulder after flowing out of the dyeing box;
the periphery of the shaking table surrounds the adapter sleeve, the periphery of the adapter sleeve surrounds the drainage box, the upper part of the adapter sleeve is provided with the spacer ring to separate the drainage ring from the lower part of the adapter sleeve, a recess is arranged below the adapter sleeve, the second handle is arranged in the recess, and the adapter sleeve is driven to move by the second handle, so that the on-off of fluid between the drainage ring and the drainage box is realized;
the circumferential surface of the dyeing box comprises two planes and two cambered surfaces; the inner wall of the dyeing box is provided with a plurality of supporting pieces, and the supporting pieces are distributed on the three height surfaces and used for placing pieces to be dyed;
one part of the lower surface of the adapter ring is supported on the support plate, the other part of the lower surface of the adapter ring is connected with the adapter sleeve through four connecting rods, and the connecting rods are arranged in a cavity between the gas dispersing box and the dyeing bin; the outer surface of the adapter ring is provided with an upper clamping ring which is clamped into the four boxes in a sliding manner, the outer surface of the adapter sleeve is provided with a lower clamping ring which is clamped into the drainage box in a sliding manner; the dyeing machine is characterized in that the motor is arranged above the through-flow cover, the through-flow cover and the switching ring are driven to rotate through the rotation of the motor, the switching sleeve is driven to rotate through the connecting rod, the gas diffusion box can be taken out of the main body, the dyeing bin is provided with an opening, and after the gas diffusion box is taken out, the dyeing box can be placed into the dyeing bin through the opening from a gap of the connecting rod.
Preferably, the drainage box has four drainage boxes.
Preferably, partition plates are arranged among the four liquid discharge boxes.
Preferably, the height of the upper surface of the through-flow cover is the same as the height of the upper surface of the adapter ring.
Preferably, the upper cover is detachably connected with the dyeing bin.
Preferably, the first handle and the second handle are cylindrical structures.
Preferably, the dyeing box is connected with the dyeing bin through a connecting shaft.
Preferably, the connecting shafts are positioned at two sides of the dyeing box.
Preferably, the staining box can swing around the connecting shaft.
Preferably, the body is of circular configuration.
The invention has the beneficial effects that:
1. aiming at the 1 st point of the background technology, the integrated design is adopted, a plurality of processes are integrated into one device, and a dyeing box, a shaking table, a liquid storage box, a liquid discharge box, an exhaust box and the like are integrated into one device and operate organically and efficiently.
2. Against the background art 2 nd point
One) through the design of adapter ring for the adapter ring is connected with different liquid storage boxes in proper order, thereby constructs the route of the steerable break-make of multiunit, has avoided the use of a large amount of remote control valves, and whole device has only used a valve, the cost that has significantly reduced.
And secondly) through the design of the adapter sleeve, the adapter sleeve is divided into two parts, one part is a through-flow part, the other part is a rotating part, the two parts are mutually separated, the through-flow part is connected with the groove, the other end of the through-flow part is connected with the liquid discharge box, and the circulation on-off of a flow path is realized.
3. In the point 3 provided by the background technology, a complete and simple flow path is formed by a simple and effective flow path structure, such as a guide plate, a converted liquid inlet, a liquid inlet space and the like, so that a great number of problems caused by a complex flow path structure are avoided.
4. According to the 4 th point provided by the background technology, the junction surface and the groove are designed on the upper surface of the shaking table, the junction surface is connected with the dyeing box, the height drop is realized through the groove so as to facilitate through flow, and meanwhile, the junction surface is more matched with the size of the dyeing box.
5. In the point 5 proposed by the background art, the cross section of the dyeing box is designed to be a combination of two straight lines and two arc lines, so that the space in one direction is reduced, and enough space is reserved for shaking.
6. Aiming at the 6 th point proposed by the background art, a removable gas diffusion box is designed, and waste gas is dispersed and taken out for cleaning after the experiment is finished.
7. Aiming at the 7 th point of the background technology, the design of a guide plate, a groove and the like is adopted, and gradient difference flow and buffer flow are provided.
Note: the foregoing designs are not sequential, each of which provides a distinct and significant advance in the present invention over the prior art.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a top view of the invention
FIG. 2 is a front view of the structure of the present invention
FIG. 3 is a bottom view of the present invention
FIG. 4 is a three-dimensional schematic view of the staining box of the present invention
In the figures, the reference numerals are as follows:
1. the dyeing machine comprises a main body, 2, a fixed liquid box, 3, a silver dyeing liquid box, 4, a developing liquid box, 5, a washing liquid box, 6, a partition plate, 7, an adapter ring, 8, a dyeing bin, 9, a dyeing box, 10, a connecting shaft, 11, an air dispersing box, 12, a liquid discharging box, 13, a first handle, 14, a through flow cover, 15, an upright post, 16, a support leg, 17, an upper cover, 18, a guide plate, 19, a liquid inlet, 20, an upper hole, 21, a support plate, 22, an air vent, 23, a groove, 24, a convex shoulder, 25, a liquid discharging ring, 26, a second handle, 27, a shaking table, 28, a recess, 29, a partition ring, 30, an adapter sleeve, 31, a plane, 32, an arc surface, 33, a support member, 34, a control valve, 35, a connecting rod, 36, a motor, 37, an upper clamping ring, 38 and a lower clamping ring.
Detailed Description
As shown in the figure: an automatic dyeing and decoloring device comprises a main body, a fixed liquid box, a silver dyeing liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a supporting leg, an upper cover, a liquid inlet, a supporting plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a supporting piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle;
the main body is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, four spaces are divided by the four clapboards, the fixed liquid box, the silver staining liquid box, the color developing liquid box and the washing liquid box are respectively accommodated in the four spaces, the bottom surfaces of the four boxes do not completely cover the supporting plate, the supporting plate is also provided with the adapter ring, the four boxes surround the adapter ring, the inner sides of the four boxes are respectively provided with a liquid inlet, the outer wall of the adapter ring is provided with an opening, and the opening is sequentially communicated with the four liquid inlets through the rotation of the adapter ring; a first handle is arranged above the adapter ring, the through-flow cover is connected to the inner side of the adapter ring, the inner side of the adapter ring is in fluid communication with the inner side of the through-flow cover, an upright post is arranged below the through-flow cover, a support leg is arranged below the upright post, the height of the upper surface of the edge of the through-flow cover is the same as that of the upper surface of the support leg, the support leg is supported on the upper cover, and the guide plate is arranged below the upper hole of the upper cover;
as shown in the figure: the gas dispersing box is arranged below the supporting plate, surrounds the dyeing bin, is provided with the vent hole on the inner side and is internally provided with the dyeing box; the control valve is arranged below the dyeing box and controls the fluid communication between the inside and the outside of the dyeing box; the dyeing box is supported on the rocking bed, a convex shoulder and a groove are arranged on the upper surface of the rocking bed, and liquid flows into the groove through the convex shoulder after flowing out of the dyeing box;
the periphery of the shaking table surrounds the adapter sleeve, the periphery of the adapter sleeve surrounds the drainage box, the upper part of the adapter sleeve is provided with the spacer ring to separate the drainage ring from the lower part of the adapter sleeve, a recess is arranged below the adapter sleeve, the second handle is arranged in the recess, and the adapter sleeve is driven to move by the second handle, so that the on-off of fluid between the drainage ring and the drainage box is realized;
the circumferential surface of the dyeing box comprises two planes and two cambered surfaces; the inner wall of the dyeing box is provided with a plurality of supporting pieces, and the supporting pieces are distributed on the three height surfaces and used for placing pieces to be dyed;
as shown in the figure: one part of the lower surface of the adapter ring is supported on the support plate, the other part of the lower surface of the adapter ring is connected with the adapter sleeve through four connecting rods, and the connecting rods are arranged in a cavity between the gas dispersing box and the dyeing bin; the outer surface of the adapter ring is provided with an upper clamping ring which is clamped into the four boxes in a sliding manner, the outer surface of the adapter sleeve is provided with a lower clamping ring which is clamped into the drainage box in a sliding manner; the dyeing machine is characterized in that the motor is arranged above the through-flow cover, the through-flow cover and the switching ring are driven to rotate through the rotation of the motor, the switching sleeve is driven to rotate through the connecting rod, the gas diffusion box can be taken out of the main body, the dyeing bin is provided with an opening, and after the gas diffusion box is taken out, the dyeing box can be placed into the dyeing bin through the opening from a gap of the connecting rod.
As shown in the figure: the number of the drainage boxes is four. And partition plates are arranged among the four liquid discharge boxes. The height of the upper surface of the through-flow cover is the same as that of the upper surface of the adapter ring. The upper cover is detachably connected with the dyeing bin. The first handle and the second handle are of cylindrical structures. The dyeing box is connected with the dyeing bin through a connecting shaft. The connecting shafts are positioned on two sides of the dyeing box. The dyeing box can swing around the connecting shaft. The main body is of a circular structure.
The working principle is as follows: the supporting pieces are perpendicular to the circumferential surface of the dyeing box in a working state, the supporting pieces can rotate upwards to expand the internal space of the dyeing box, and three pieces of pieces to be dyed are placed corresponding to three height surfaces and are supported on the corresponding supporting pieces; taking the gas diffusion box out of the main body, placing the dyeing box into a dyeing bin through the opening from the gap of the four connecting rods, overlapping the guide plate on the dyeing box due to the action of gravity, and finally placing the gas diffusion box back to the main body;
the liquid feeding method comprises the following steps: the motor is operated to rotate to drive the through-flow cover and the adapter ring to rotate, so that the through ports on the adapter ring are sequentially communicated with the four liquid inlets, different liquids are sequentially introduced into the dyeing box, and the liquids sequentially flow through the liquid inlets, the through ports, the through-flow cover, the upper hole and the guide plate to enter the dyeing box;
the liquid discharging method comprises the following steps: the motor drives the adapter ring to rotate, and simultaneously drives the adapter sleeve to synchronously rotate through the connecting rod, so that the liquid discharge ring is communicated with the liquid discharge box; secondly, controlling the control valve to be opened, and enabling liquid in the dyeing box to flow through the convex shoulder, the groove and the liquid discharge ring in sequence to enter the liquid discharge box;
the exhaust method comprises the following steps: after the control valve is controlled to be opened, waste gas enters the air dispersing box through the opening and the vent hole, and the air dispersing box is taken out from the main body for cleaning after the work is finished.
The above detailed description is specific to possible embodiments of the present invention, and the embodiments are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the scope of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. The utility model provides an automatic change dyeing decoloration device which characterized in that: the device comprises a main body, a fixed liquid box, a silver dye liquid box, a developing liquid box, a washing liquid box, a partition plate, a switching ring, a dyeing bin, a dyeing box, a connecting shaft, an air dispersing box, a liquid discharging box, a first handle, a through flow cover, an upright post, a support leg, an upper cover, a liquid inlet, a support plate, an air vent, a groove, a convex shoulder, a shaking table, a switching sleeve, a support piece, a control valve, a motor, a connecting rod, an upper clamping ring and a lower clamping ring; wherein the upper cover is provided with an upper hole and a guide plate; the adapter sleeve comprises a liquid discharge ring, a spacer ring, a recess and a second handle;
the main body is internally provided with a supporting plate, four clapboards are arranged above the supporting plate, four spaces separated by the four clapboards are respectively used for accommodating the fixed liquid box, the silver staining liquid box, the developing liquid box and the washing liquid box, the bottom surfaces of the four boxes do not completely cover the supporting plate, the supporting plate is also provided with the adapter ring, the four boxes surround the adapter ring, the inner sides of the four boxes are respectively provided with a liquid inlet, the outer wall of the adapter ring is provided with an opening, and the opening is sequentially communicated with the four liquid inlets through the rotation of the adapter ring; a first handle is arranged above the adapter ring, the through-flow cover is connected to the inner side of the adapter ring, the inner side of the adapter ring is in fluid communication with the inner side of the through-flow cover, an upright post is arranged below the through-flow cover, a support leg is arranged below the upright post, the height of the upper surface of the edge of the through-flow cover is the same as that of the upper surface of the support leg, the support leg is supported on the upper cover, and the guide plate is arranged below the upper hole of the upper cover;
the gas dispersing box is arranged below the supporting plate, surrounds the dyeing bin, is provided with the vent hole on the inner side and is internally provided with the dyeing box; the control valve is arranged below the dyeing box and controls the fluid communication between the inside and the outside of the dyeing box; the dyeing box is supported on the shaking table, a convex shoulder and a groove are formed in the upper surface of the shaking table, and liquid flows into the groove through the convex shoulder after flowing out of the dyeing box;
the periphery of the shaking table surrounds the adapter sleeve, the periphery of the adapter sleeve surrounds the drainage box, the upper part of the adapter sleeve is provided with the spacer ring to separate the drainage ring from the lower part of the adapter sleeve, a recess is arranged below the adapter sleeve, the second handle is arranged in the recess, and the adapter sleeve is driven to move by the second handle, so that the on-off of fluid between the drainage ring and the drainage box is realized;
the circumferential surface of the dyeing box comprises two planes and two cambered surfaces; the inner wall of the dyeing box is provided with a plurality of supporting pieces, and the supporting pieces are distributed on the three height surfaces and used for placing pieces to be dyed;
one part of the lower surface of the adapter ring is supported on the support plate, the other part of the lower surface of the adapter ring is connected with the adapter sleeve through four connecting rods, and the four connecting rods are arranged in a cavity between the gas diffusion box and the dyeing bin; the outer surface of the adapter ring is provided with an upper clamping ring which is clamped into the four boxes in a sliding manner, the outer surface of the adapter sleeve is provided with a lower clamping ring which is clamped into the drainage box in a sliding manner; the through-flow is covered the top and is equipped with the motor, through the rotatory drive of motor the through-flow cover with the switching ring is rotatory, and then passes through the connecting rod drives the switching cover is rotatory, the box of loosing can be followed take out in the main part, be equipped with the opening on the dyeing storehouse, after taking out the box of loosing, the dyeing box can be followed the clearance of connecting rod passes through the opening is placed into in the dyeing storehouse.
2. The automatic dyeing and decoloring device according to claim 1, wherein: the drainage box has four.
3. An automated dyeing and decoloring apparatus according to claim 2, wherein: and partition plates are arranged among the four liquid discharge boxes.
4. The automatic dyeing and decoloring device according to claim 1, wherein: the height of the upper surface of the through-flow cover is the same as that of the upper surface of the adapter ring.
5. The automatic dyeing and decoloring device according to claim 1, wherein: the upper cover is detachably connected with the dyeing bin.
6. The automatic dyeing and decoloring device according to claim 1, wherein: the first handle and the second handle are of cylindrical structures.
7. The automatic dyeing and decoloring device according to claim 1, wherein: the dyeing box is connected with the dyeing bin through a connecting shaft.
8. The automatic dyeing and decoloring device according to claim 7, wherein: the connecting shafts are positioned on two sides of the dyeing box.
9. The automatic dyeing and decoloring device according to claim 7, wherein: the dyeing box can swing around the connecting shaft.
10. The automatic dyeing and decoloring device according to claim 1, wherein: the main body is of a circular structure.
CN202010165865.9A 2020-03-11 2020-03-11 Automatic change dyeing decoloration device Active CN111323285B (en)

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