CN111589480A - Pipetting device, micro-system pipetting system comprising pipetting device and method thereof - Google Patents
Pipetting device, micro-system pipetting system comprising pipetting device and method thereof Download PDFInfo
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- CN111589480A CN111589480A CN202010475700.1A CN202010475700A CN111589480A CN 111589480 A CN111589480 A CN 111589480A CN 202010475700 A CN202010475700 A CN 202010475700A CN 111589480 A CN111589480 A CN 111589480A
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004140 cleaning Methods 0.000 claims abstract description 165
- 239000007788 liquid Substances 0.000 claims abstract description 55
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 39
- 238000012546 transfer Methods 0.000 claims abstract description 17
- 238000011010 flushing procedure Methods 0.000 claims abstract description 11
- 230000009471 action Effects 0.000 claims abstract description 8
- 238000005406 washing Methods 0.000 claims description 14
- 238000005213 imbibition Methods 0.000 claims description 7
- 230000007246 mechanism Effects 0.000 claims description 6
- 230000000694 effects Effects 0.000 abstract description 8
- 238000003205 genotyping method Methods 0.000 abstract description 5
- 238000013461 design Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000002791 soaking Methods 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 9
- 238000011001 backwashing Methods 0.000 description 6
- 238000007789 sealing Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000002000 scavenging effect Effects 0.000 description 4
- 238000012864 cross contamination Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/023—Cleaning the external surface
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/0321—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing using pressurised, pulsating or purging fluid
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- Mechanical Engineering (AREA)
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Abstract
The invention discloses a liquid transfer device, a micro-system liquid transfer system comprising the liquid transfer device and a method thereof. The inner wall high-pressure cleaning is more time-saving than the suction and spitting actions of the conventional suction head, the cleaning effect is better, the cleaning method is quicker than the replacement of the suction head, the cost is saved, and the requirement of the genotyping device on more rounds of cleaning in shorter time can be met. The outer wall is washed with two sides of dynamic running water and the middle part of the dynamic running water, so that the cleaning effect is better than that of the conventional soaking plastic suction head, liquid after cleaning and mutual pollution of needle points of different channels can be prevented. The invention adopts the combined design of high-pressure cleaning of the inner wall of the pipetting head and opposite-flushing type cleaning of the outer wall, reduces the cost for replacing the plastic pipette head, reduces the time spent on cleaning, and meets the requirements of high throughput, multi-site, rapidness and low cost of pipetting technology in a genotyping system.
Description
Technical Field
The invention relates to the technical field of gene detection, in particular to a liquid transfer device, a micro-system liquid transfer system comprising the liquid transfer device and a method thereof.
Background
In genotyping systems, it is often necessary to transfer samples or reagents from one vessel to another, the carrier of the transfer technique typically being a plastic tip. In order to prevent cross contamination among different samples or reagents, the suction head is often replaced or soaked under the liquid level of pure water and the suction head is sucked and discharged, so that the cross contamination in the pipetting process of the suction head cannot influence the final typing result.
However, if the conventional plastic tip pipetting scheme is used for tip replacement, a large number of disposable tip replacements represent a large cost for some customers who have a non-obvious need for cross-contamination control.
For the customers who adopt the cleaning scheme, the cleaning scheme at the present stage is often too long, and can be as long as several minutes. In most genetic testing and typing systems, in order to ensure the testing flux, the number of channels related to pipetting is usually a multiple of 4, namely 4 channels, 8 channels, 12 channels or higher, so as to meet the requirements of timeliness and high flux. In a part of microsystems, the reaction liquid after the sample and the reagent are mixed is generally less than 3ul, so that the cost is saved during the large-batch test, and the method is common in some scientific research institutions for parting. Under the conventional temperature environment, the time is 10 minutes, the volatilization volume of water molecules in a 384-pore plate can reach about 0.3ul, so that the micro-system construction time is controlled within 5 minutes or even shorter time, the conventional cleaning scheme is not facilitated, the combination mode of a sample and a reagent is also restricted, the situation that the pipetting time is too long, the volatilization amount of liquid in a pipetted pore is too large, and the final typing result is seriously influenced is avoided.
Disclosure of Invention
The present invention is directed to solving the above problems and to providing a pipetting device, a micro-system pipetting system including the same, and a method thereof.
In order to achieve the above object, the present disclosure provides a pipette device including:
the plunger rod and a driving element for driving the plunger rod to move up and down; the plunger rod is fixed with the driving element;
a first chamber; the plunger rod is matched with the first chamber, and the gas volume of the first chamber is changed when the plunger rod moves up and down;
a second chamber and a pipetting head; first cavity and second cavity intercommunication, second cavity with move liquid head intercommunication, when the gas volume increase of first cavity, move liquid head and realize the imbibition, when the gas volume of first cavity diminishes, move liquid head and realize the flowing back.
The present disclosure further provides a micro system pipetting system, comprising the pipetting device, wherein the first chamber and the second chamber are communicated through a conduit I, and further comprising an inner wall cleaning device and an opposite flushing cleaning device;
the inner wall cleaning device comprises a cleaning pipeline I, an inner wall cleaning pump and an inner wall cleaning valve, wherein a first end of the cleaning pipeline I is connected with a cleaning water source, a second end of the cleaning pipeline I is communicated with the second chamber through a conduit II, the inner wall cleaning pump is arranged on the cleaning pipeline I, and the inner wall cleaning valve is arranged on the conduit II;
the hedging cleaning device comprises a cleaning pipeline II, an outer wall cleaning pump, an outer wall cleaning valve and hedging openings, wherein the hedging openings are formed in the two sides of a pipetting head, a first end of the cleaning pipeline II is connected with a cleaning water source, a second end of the cleaning pipeline II is communicated with the hedging openings, the outer wall cleaning pump and the outer wall cleaning valve are arranged on the cleaning pipeline II, and the outer wall cleaning pump is close to the cleaning water source.
The present disclosure further provides a micro system pipetting method, applied to the micro system pipetting system, including the following steps:
s1, the pipetting head is inserted into the cleaning pool under the action of a power source, the lower end of the pipetting head is positioned below the opposite flushing port, and the plunger rod descends to the initial pipetting position in the first chamber;
s2, opening the inner wall cleaning valve and the outer wall cleaning valve at the same time;
s3, opening the inner wall cleaning pump and the outer wall cleaning pump at the same time after delaying;
s4, after keeping for a certain time, closing the inner wall cleaning pump;
s5, closing the inner wall cleaning valve after time delay;
and S6, closing the outer wall cleaning pump after time delay.
The invention has the beneficial effects that:
the invention relates to a liquid transfer device, a micro-system liquid transfer system comprising the liquid transfer device and a method thereof, which solve the problem of multi-site system construction, adopt the combined design of high-pressure cleaning of the inner wall and opposite flushing type cleaning of the outer wall of a liquid transfer head in the scene of long-time liquid transfer operation, reduce the cost of replacing a plastic suction head, reduce the time spent on cleaning, and meet the requirements of high throughput, multi-site, rapidness and low cost of the liquid transfer technology in a genotyping system.
Other features of the invention will be set forth in the detailed description which follows, and in part will be obvious from the description, the claims, and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the disclosure and are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description serve to explain the disclosure without limiting the disclosure. In the drawings:
FIG. 1 is a schematic view of the structure of a pipetting device according to the invention;
FIG. 2 is a cross-sectional view of a pipetting device according to the invention;
FIG. 3 is a flow chart of the pipetting described in the present invention;
FIG. 4 is a fluid path connection diagram of the micro-system pipetting system of the present invention;
FIG. 5 is a flow chart of the cleaning according to the present invention;
fig. 6 is a schematic illustration of the cleaning according to the present invention.
Description of the reference numerals
1-motor, 2-conduit II, 3-upper seat, 4-conduit I, 5-lower seat, 6-liquid transferring head, 7-cleaning pool, 8-waste liquid container, 9-cleaning pipeline II, 10-outer wall cleaning valve, 11-outer wall cleaning pump, 12-filter, 13-inner wall cleaning pump, 14-water tank, 15-cleaning pipeline I, 16-shunt valve, 17-inner wall cleaning valve, 18-motor mounting plate, 19-optical coupler, 20-screw rod, 21-positioning shaft, 22-transmission block, 23-shaft fixing plate, 24-conical washer, 25-sealing cover plate, 26-O-shaped ring, 27-opposite flushing port, 28-water outlet, 29-plunger rod and 30-spring.
Detailed Description
The following detailed description of specific embodiments of the present disclosure is provided in connection with the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating the present disclosure, are given by way of illustration and explanation only, not limitation.
In the present disclosure, unless otherwise specified, the terms of orientation such as "upper" and "lower" are generally defined based on the orientation or positional relationship shown in the drawings, and specifically refer to the direction of the drawing shown in fig. 1 in combination with the positional relationship of the corresponding parts in other drawings. Furthermore, the terms "first," "second," and the like, as used in this disclosure, are intended to distinguish one element from another, and not necessarily for order or importance. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated.
As shown in fig. 1 and 2, the present invention relates to a pipette device including:
a plunger rod 29 and a driving member for driving the plunger rod 29 to move up and down; the plunger rod 29 is fixed with the driving member;
a first chamber; the plunger rod 29 is arranged in a manner of being matched with the first chamber, and the gas volume of the first chamber is changed when the plunger rod 29 moves up and down;
a second chamber and pipetting head 6; first cavity and second cavity intercommunication, second cavity with move liquid head 6 intercommunication, when the gas volume increase of first cavity, move liquid head 6 and realize the imbibition, when the gas volume of first cavity diminishes, move liquid head 6 and realize the flowing back.
Optionally, the pipetting device comprises 12 first chambers, 12 second chambers, 12 plunger rods 29 and 12 pipetting heads 6, one first chamber being arranged for one second chamber, one plunger rod 29 and one pipetting head 6.
Alternatively, 12 first chambers are all arranged on the upper seat 3 in an upward opening manner, 12 second chambers are all arranged on the lower seat 5 in a downward opening manner, the upper seat 3 is connected with the lower seat 5 through a spring 30, a plunger rod 29 is downwards inserted into the first chambers, and the pipetting head 6 is hermetically connected to the lower parts of the second chambers.
Optionally, the driving element includes a motor 1 and a screw mechanism, the motor 1 is connected to a screw 20 of the screw mechanism, the plunger rod 29 is fixed relative to a driving nut of the screw mechanism, the motor 1 drives the screw 20 to rotate, and the driving nut drives the plunger rod 29 to move up and down.
Specifically, the motor 1 is a stepping motor 1, and the motor 1 is fixedly mounted on the motor mounting plate 18. An optical coupler 19 is arranged on the lower side surface of the motor mounting plate 18. The screw rod 20 penetrates through the motor mounting plate 18 and is connected with the motor 1. The transmission nut is fixed on the transmission block 22 and is matched with the screw rod 20. The transmission block 22 is connected with the motor mounting plate 18 through two vertically arranged positioning shafts 21 for positioning, the upper end of the positioning shaft 21 is slidably connected with the motor mounting plate 18, and the lower end of the positioning shaft 21 is fixedly connected with the transmission block 22. When the motor 1 drives the screw rod 20 to coaxially rotate, the transmission block 22 passively moves up and down, and the positioning shaft 21 moves up and down relative to the motor mounting plate 18. The shaft fixing plate 23 is provided with 12 countersunk through holes, the upper end part of the plunger rod 29 is outwards protruded to be provided with a limiting ring, the plunger rod 29 downwards passes through the countersunk through holes, the limiting ring is downwards limited in the countersunk through holes, the upper side surface of the shaft fixing plate 23 and the lower side surface of the transmission block 22 are fixedly installed, namely the limiting ring is limited between the shaft fixing plate 23 and the transmission block 22 up and down, so that the transmission block 22 drives the plunger rod 29 to synchronously move up and down when moving up and down. The 12 plunger rods 29 penetrating through the shaft fixing plate 23 penetrate through the sealing cover plate 25 and are respectively sleeved with the O-rings 26, then the lower ends of the plunger rods 29 are inserted into the first cavity of the upper seat 3, the sealing cover plate 25 is fixed on the upper side surface of the upper seat 3, the O-rings 26 are pressed between the sealing cover plate 25 and the upper seat 3, the plunger rods 29 perform piston movement relative to the upper seat 3 and the sealing cover plate 25, and the plunger rods 29 are in sealing connection with the first cavity to prevent the plunger rods 29 from leaking when moving up and down or cleaning. 12 first chambers configured on the upper seat 3 are mutually independent, 12 second chambers configured on the lower seat 5 are mutually independent, the first chambers and the second chambers are communicated through a guide pipe I4, and the side parts of the first chambers and the second chambers are sealed with a guide pipe I4 by adopting nut joints and tapered gaskets 24. The bottom of the second chamber is hermetically connected with the pipetting head 6. When the motor 1 drives the plunger rod 29 to move up and down, the air volume of the first chamber of the upper seat 3 changes, and further the pressure changes, and the pressure changes are transmitted to the second chamber of the lower seat 5 through the conduit I4, so that the piston movement of the plunger rod 29 is gradually converted into the liquid sucking and discharging action of the liquid moving head 6.
The pipetting scheme is shown in FIG. 3 and comprises the following steps:
the method comprises the following steps: first, a cleaning process is performed.
Step two: move the liquid-transfering device to imbibition position top through the arm to go deep into to the liquid level below, step motor 1 drives plunger rod 29 to move up a certain stroke simultaneously, realizes the action of breathing in before the imbibition to prevent to wash remaining washing water and the liquid after the imbibition in the liquid-transfering head 6 after the imbibition from melting pollution and diluting each other, the action of breathing in needs to be accomplished before stretching into the liquid level below.
Step three: under the liquid level, the stepping motor 1 drives the plunger rod 29 to move upwards for a certain stroke so as to suck a designated volume of liquid.
Step four: after the liquid is sucked, the liquid transferring head 6 is kept under the liquid level, so that the motor 1 drives the plunger rod 29 to move downwards for a small stroke, and the return stroke difference of the screw rod 20 is eliminated.
Step five: the pipetting device is lifted off the liquid level by the mechanical arm and is lifted to the vertical initial position.
Step six: the liquid-transfering device moves to the flowing back hole site through the arm to with the flowing back hole contact.
Step seven: the stepping motor 1 drives the plunger rod 29 to move downwards for a specified stroke, so that a multichannel simultaneous liquid drainage function with a specified volume is realized.
Step eight: after the pipetting function is completed, a washing process is executed.
Step nine: and (6) ending.
Move liquid device can realize the demand that the multichannel contact moved liquid simultaneously, and possess the anticollision function, and is better to moving liquid head 6's protection effect.
In addition, when a plurality of rows are needed, only the step six and the step seven need to be executed repeatedly, and after all the pipettes are finished, the step eight and the step nine need to be executed.
The micro-system pipetting system comprises the pipetting device, a first chamber and a second chamber which are communicated through a conduit I4, an inner wall cleaning device and an opposite flushing cleaning device;
the inner wall cleaning device comprises a cleaning pipeline I15, an inner wall cleaning pump 13 and an inner wall cleaning valve 17, wherein the first end of the cleaning pipeline I15 is connected with a cleaning water source, the second end of the cleaning pipeline I15 is communicated with the second chamber through a conduit II2, the inner wall cleaning pump 13 is arranged on the cleaning pipeline I15, and the inner wall cleaning valve 17 is arranged on a conduit II 2;
offset belt cleaning device is including wasing pipeline II9, outer wall scavenging pump 11, outer wall cleaning valve 10 and offset 27, move liquid head 6 both sides and all be provided with offset 27, wash pipeline II 9's first end and washing water source and be connected, wash pipeline II 9's second end and offset 27 intercommunication, set up outer wall scavenging pump 11 on the washing pipeline II9, outer wall scavenging valve 10, outer wall scavenging pump 11 is close to the setting of washing water source.
Optionally, the inner wall cleaning device further comprises a filter 12, the filter 12 being arranged on the cleaning line I15.
Optionally, each second chamber is correspondingly connected with a conduit II2, the cleaning pipeline I15 is respectively connected to the diverter valves 16 after passing through the multi-way joints, and each diverter of the diverter valve 16 is communicated with one conduit II 2.
Specifically, as shown in fig. 4, the inner wall cleaning water is firstly sucked out from the water tank 14 by the inner wall cleaning pump 13, pressurized to 1-2.5 bar, and then subjected to cleaning treatment by the filter 12, so as to complete source supply of the inner wall cleaning water. The water source supplied from the front end is divided into three parts by the four-way joint and then respectively connected to three inlets of the diverter valve 16, and is relatively uniformly distributed to each guide pipe II2 through the diversion function of the diverter valve 16, when the inner wall cleaning valve 17 on the guide pipe II2 is opened, the flow after diversion treatment enters the second chamber of the liquid-moving device through the guide pipe II2, and the inner wall high-pressure cleaning is completed.
Optionally, the hedging cleaning device further comprises a cleaning pool 7, the pipetting head 6 is located in the cleaning pool 7, the hedging openings 27 are formed in two sides of the cleaning pool 7, and water flows of the two sides of the hedging openings 27 act on the pipetting head 6 in a crossed mode.
Optionally, a drain 28 is provided at the bottom of the washing tank 7.
Specifically, as shown in fig. 4 and 5, the outer wall backwashing water is firstly sucked out from the water tank 14 by the outer wall washing pump 11, and then reaches the outer wall washing tank 7 through the outer wall washing valve 10, the outer wall backwashing water enters from the 6 water inlet joints and then passes through the valve passage inside the outer wall washing tank 77, the valve passage divides the outer wall backwashing water, the outer wall backwashing water flows out from the 24 backwashing holes 27 on both sides inside the washing tank 7 in a relatively uniform manner, so as to achieve the backwashing effect, and the waste liquid flows out from the water outlet 28.
The micro system pipetting method of the present invention is applied to the micro system pipetting system, as shown in fig. 6, and includes the following contents:
s1, the pipetting head 6 is inserted into the washing pool 7 under the action of a power source, the lower end of the pipetting head 6 is positioned below the counter flushing port 27, and the plunger rod 29 descends to a pipetting initial position in the first chamber;
s2, opening the inner wall cleaning valve 17 and the outer wall cleaning valve 10 simultaneously;
s3, opening the inner wall cleaning pump 13 and the outer wall cleaning pump 11 at the same time after delaying;
s4, after keeping for a certain time, closing the inner wall cleaning pump 13;
s5, closing the inner wall cleaning valve 17 after delaying;
s6, the outer wall cleaning pump 11 is turned off after a delay.
Specifically, the cleaning process comprises the following steps:
the method comprises the following steps: the liquid-transfering module is fixed on the corresponding mechanical arm, and moves it to the vertical initial position directly over the cleaning pool 7 through the mechanical arm, and simultaneously controls the step motor 1 to rotate, drives the transmission block 22 to move up to the signal detection position of the optical coupler 19, and at this moment, the plunger rod 29 moves up to the upper limit position of the first type chamber of the upper seat 3.
Step two: the needle point of the liquid transferring head 6 is extended into the cleaning pool 7 to flush the lower part of the water outlet hole and is at a certain distance from the bottom of the cleaning pool 7, and meanwhile, the stepping motor 1 is controlled to drive the plunger rod 29 to move downwards to a designated position at the lower end of the first chamber, and the position is defined as the liquid absorbing initial position of the liquid absorbing plunger rod 29.
Step three: the inner wall cleaning valve 17 and the outer wall cleaning valve 10 are opened at the same time.
Step four: after a certain time delay, the inner wall cleaning pump 13 and the outer wall cleaning pump 11 are simultaneously started, and then the starting state is kept for a certain time. At this time, the outer wall cleaning water passes through the outer wall cleaning pump 11 and the outer wall cleaning valve 10, and then is sprayed out from two sides in the outer wall cleaning tank 7 through the flow channel of the outer wall cleaning tank 7, and because the water outlets at two sides present the design of an included angle of 150-180 degrees, the water flow is wholly sprayed out towards the outer wall of the liquid moving head 6 under the dual constraints of the spraying direction and gravity, so that the array type hedging cleaning effect of the outer wall is realized. And the inner wall cleaning water is acted by the inner wall cleaning pump 13 and the inner wall cleaning valve 17, the pressure of the water towards the front end of the water outlet of the liquid transfer head 6 can reach about 1-2 bar, and then the water is sprayed out through the inner wall of the liquid transfer head 6 at a relatively uniform flow rate, so that the effect of high-pressure cleaning of the inner wall is realized.
Step five: after a certain period of time of cleaning the inner and outer walls, the inner wall cleaning pump 13 is first turned off.
Step six: after 0.5-3 s of time delay, the inner wall cleaning valve 17 is closed, so that excessive pressure is not remained in the inner wall pipeline as far as possible, and the excessive pressure is more than 1 ba.
Step seven: and after continuing delaying for 0.5-3 s, closing the outer wall cleaning pump 11.
Step eight: after 0.5-3 s of time delay is carried out on the basis of the previous step, the outer wall cleaning valve 10 is closed, the inner wall cleaning valve 17 is closed firstly, and then the outer wall cleaning valve 10 is closed, so that the phenomenon that liquid is hung at the liquid transferring head 6 after the inner wall is cleaned and stopped is mainly prevented.
Step nine: and then the pipetting device is lifted to a reset position above the cleaning pool 7 through the mechanical arm to complete the whole set of cleaning process.
Further, the post-cleaning liquid generated during the cleaning process flows out to the predetermined waste liquid container 8 through the drain port 28 at the bottom of the cleaning tank 7.
The inner wall high-pressure cleaning is more time-saving than the suction and spitting actions of the conventional suction head, the cleaning effect is better, the cleaning method is quicker than the replacement of the suction head, the cost is saved, and the requirement of the genotyping device on more rounds of cleaning in shorter time can be met.
The outer wall is washed with two sides of dynamic running water and the middle part of the dynamic running water, so that the cleaning effect is better than that of the conventional soaking plastic suction head, liquid after cleaning and mutual pollution of needle points of different channels can be prevented.
The preferred embodiments of the present disclosure are described in detail with reference to the accompanying drawings, however, the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present disclosure within the technical idea of the present disclosure, and these simple modifications all belong to the protection scope of the present disclosure.
It should be noted that, in the foregoing embodiments, various features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various combinations that are possible in the present disclosure are not described again.
In addition, any combination of various embodiments of the present disclosure may be made, and the same should be considered as the disclosure of the present disclosure, as long as it does not depart from the spirit of the present disclosure.
Claims (10)
1. Pipetting device, characterized by comprising:
the plunger rod and a driving element for driving the plunger rod to move up and down; the plunger rod is fixed with the driving element;
a first chamber; the plunger rod is matched with the first chamber, and the gas volume of the first chamber is changed when the plunger rod moves up and down;
a second chamber and a pipetting head; first cavity and second cavity intercommunication, second cavity with move liquid head intercommunication, when the gas volume increase of first cavity, move liquid head and realize the imbibition, when the gas volume of first cavity diminishes, move liquid head and realize the flowing back.
2. Pipetting device according to claim 1, characterized in that the pipetting device comprises n first chambers, n second chambers, n plunger rods and n pipetting heads, where n is a positive integer, one first chamber being arranged for each second chamber, one plunger rod and one pipetting head.
3. A pipetting device as recited in claim 2 wherein n first chambers are each provided with an upward opening in the upper base, n second chambers are each provided with a downward opening in the lower base, the upper base and the lower base are connected by a spring, the plunger rod is inserted downward into the first chambers, and the pipetting head is sealingly connected to the lower part of the second chambers.
4. A liquid transfer device according to claim 1, characterized in that the driving member comprises a motor and a screw mechanism, the motor is connected with a screw of the screw mechanism, the plunger rod is fixed relative to a driving nut of the screw mechanism, the motor drives the screw to rotate, and the driving nut drives the plunger rod to move up and down.
5. The micro-system pipetting system comprising the pipetting device as recited in any one of claims 1 to 4, wherein the first chamber and the second chamber are communicated through a conduit I, and further comprising an inner wall washing device and a counter washing device;
the inner wall cleaning device comprises a cleaning pipeline I, an inner wall cleaning pump and an inner wall cleaning valve, wherein a first end of the cleaning pipeline I is connected with a cleaning water source, a second end of the cleaning pipeline I is communicated with the second chamber through a conduit II, the inner wall cleaning pump is arranged on the cleaning pipeline I, and the inner wall cleaning valve is arranged on the conduit II;
the hedging cleaning device comprises a cleaning pipeline II, an outer wall cleaning pump, an outer wall cleaning valve and hedging openings, wherein the hedging openings are formed in the two sides of a pipetting head, a first end of the cleaning pipeline II is connected with a cleaning water source, a second end of the cleaning pipeline II is communicated with the hedging openings, the outer wall cleaning pump and the outer wall cleaning valve are arranged on the cleaning pipeline II, and the outer wall cleaning pump is close to the cleaning water source.
6. The micro-system pipetting system of claim 5, wherein the interior wall cleaning device further comprises a filter disposed on the cleaning line I.
7. The micro-system pipetting system of claim 5, wherein each second chamber is correspondingly connected with a conduit II, the cleaning pipeline I is respectively connected with a diverter valve after passing through the multi-way joint, and each diverter port of the diverter valve is communicated with one conduit II.
8. The micro-system pipetting system of claim 5, wherein the counter-flushing cleaning device further comprises a cleaning tank, the pipetting head is located in the cleaning tank, the counter-flushing ports are arranged on two sides of the cleaning tank, and water flows of the counter-flushing ports on the two sides act on the pipetting head in a cross way.
9. The micro-system pipetting system of claim 8, wherein the bottom of the wash basin is provided with a drain.
10. A microsystem pipetting method, characterized in that: the micro system pipetting system applied to any one of claims 5 to 9, comprising the following:
s1, the pipetting head is inserted into the cleaning pool under the action of a power source, the lower end of the pipetting head is positioned below the opposite flushing port, and the plunger rod descends to the initial pipetting position in the first chamber;
s2, opening the inner wall cleaning valve and the outer wall cleaning valve at the same time;
s3, opening the inner wall cleaning pump and the outer wall cleaning pump at the same time after delaying;
s4, after keeping for a certain time, closing the inner wall cleaning pump;
s5, closing the inner wall cleaning valve after time delay;
and S6, closing the outer wall cleaning pump after time delay.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010475700.1A CN111589480A (en) | 2020-05-29 | 2020-05-29 | Pipetting device, micro-system pipetting system comprising pipetting device and method thereof |
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