CN206531706U - A kind of polyacrylamide gel silver staining display device - Google Patents
A kind of polyacrylamide gel silver staining display device Download PDFInfo
- Publication number
- CN206531706U CN206531706U CN201720009245.XU CN201720009245U CN206531706U CN 206531706 U CN206531706 U CN 206531706U CN 201720009245 U CN201720009245 U CN 201720009245U CN 206531706 U CN206531706 U CN 206531706U
- Authority
- CN
- China
- Prior art keywords
- cylinder body
- silver staining
- outage
- polyacrylamide gel
- display device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a kind of polyacrylamide gel silver staining display device, including open-topped cylinder body, it is provided with some to vertical neck on the narrow side wall of the cylinder body, the bottom of cylinder body broad side walls offers outage, outage is provided with draining switch, and is connected with discharging tube.By setting multiple vertical necks corresponding two-by-two on the relatively narrow side wall of cylinder body, it can be achieved to carry out silver staining color operation to polylith offset plate simultaneously, substantially increase operating efficiency.In addition, the present apparatus frequently changes and toppled over the defect of decoction during traditional silver staining by instead of the outflow of decoction from cylinder body broad side walls bottom outage, on the one hand the proof strength of testing crew is reduced, the security hidden trouble that easily unrestrained decoction is produced to testing crew is on the other hand also largely reduced.
Description
Technical field
The utility model is related to silver staining display device technical field, more particularly to a kind of colour developing of polyacrylamide gel silver staining
Device.
Background technology
Polyacrylamide gel electrophoresis (PAGE) is a kind of electrophoresis analytical method commonly used during nucleic acid samples are analyzed.After electrophoresis
To a variety of methods that show of sample, wherein silver staining development process because toxicity is light, to pollute small, sensitivity high etc. enjoys the green grass or young crops of researcher
Look at, be used widely in the electrophoresis detection that SSR, SRAP equimolecular are marked.
At present, it is to use horizontal plastic casing that the silver staining colour developing of polyacrylamide gel is mostly.Its dyeing procedure is as follows：(1) will
The horizontal plastic casing that offset plate immersion with gel fills fixer fixes 20min；(2) offset plate is being filled into the level of rinsing liquid
Plastic casing rinses 2min；(3) offset plate is immersed to the plastic casing dyeing 30min for filling dyeing liquor；(4) offset plate is being filled into distilled water
Plastic casing in instantaneously get express developed with except removal floating color；(5) offset plate is immersed into the 10min that develops in the plastic casing for filling developer solution；
(6) offset plate is rinsed well with distilled water.
In summary, dyed by horizontal plastic casing, be only capable of dyeing one block of offset plate every time, and every block of offset plate
Whole chromogenic reaction about need 65min.When detecting that sample size is larger, dyeing work seems and wasted time and energy that operating efficiency is low.
In addition, needing frequently to change and topple over decoction in silver staining process color, not only proof strength, Er Qieqing are added to testing crew
The decoction trickled down during decoction is easily damaged to testing crew, there is certain potential safety hazard.
Utility model content
For the above mentioned problem mentioned in background technology, the purpose of this utility model is to provide a kind of time saving and energy saving safety
Polyacrylamide gel silver staining display device.
The problem of in order to solve in the presence of background technology, the technical solution of the utility model is：
On a kind of polyacrylamide gel silver staining display device, including open-topped cylinder body, the narrow side wall of the cylinder body
It is provided with some to vertical neck, the bottom of cylinder body broad side walls offers outage, and outage is provided with draining switch.
Preferably, polyacrylamide gel silver staining display device of the present utility model, wherein the both upper ends thereof of the cylinder body
Protrusion, and the bottom of protrusion is less than the height that offset plate to be dyed is inserted after cylinder body.
Preferably, polyacrylamide gel silver staining display device of the present utility model, wherein the vertical neck uniformly divides
It is distributed on the narrow side wall of cylinder body, and the width of neck is more than setting between the thickness of offset plate to be dyed, adjacent upright neck vertically
There is interval.
Preferably, polyacrylamide gel silver staining display device of the present utility model, wherein being connected with the outage
Discharging tube.
Preferably, polyacrylamide gel silver staining display device of the present utility model, wherein the top of the cylinder body is set
There is dyeing to cover.
Preferably, polyacrylamide gel silver staining display device of the present utility model, wherein outage protrusion is set
In outside the broad side walls of cylinder body.
Compared with the prior art, the beneficial effects of the utility model are：
The polyacrylamide gel silver staining display device that the utility model is provided, by being set on the relatively narrow side wall of cylinder body
Multiple vertical necks corresponding two-by-two, can be achieved to carry out silver staining color operation to polylith offset plate simultaneously, substantially increase work effect
Rate.In addition, the present apparatus is by making the outflow of decoction from cylinder body broad side walls bottom outage instead of during traditional silver staining frequently
The defect of decoction is changed and toppled over, the proof strength of testing crew is on the one hand reduced, on the other hand also largely reduces
The security hidden trouble that easily unrestrained decoction is produced to testing crew, the present apparatus can save plenty of time and manpower, safety coefficient
Height, and apparatus structure is simple and convenient to operate, is that a kind of can greatly improve the new of polyacrylamide gel silver staining colour developing efficiency
Device.
Brief description of the drawings
Fig. 1 is the structural front view of the utility model silver staining display device；
Fig. 2 is the structure top view of the utility model silver staining display device.
Wherein：1- cylinder bodies, the vertical necks of 2-, 3- outages, 4- draining switch, 5- discharging tubes, 6- dyeing lids, 7- is to be dyed
Offset plate.
Embodiment
The utility model is described in detail below in conjunction with the accompanying drawings.
As shown in Figure 1, 2, the utility model provides a kind of polyacrylamide gel silver staining display device, including top is opened
Mouthful cylinder body 1, be provided with the narrow side wall of the cylinder body 1 some to vertical neck 2, the bottom of the broad side walls of cylinder body 1 offers row
Fluid apertures 3, the protrusion of outage 3 is arranged at outside the broad side walls of cylinder body 1, and outage 3 is provided with draining switch 4, and the row of being connected with
Liquid pipe 5, draining switch 4 may also set up on discharging tube 5；The top of cylinder body 1 is provided with dyeing lid 6, the top two of the cylinder body 1
End protrusion, and the bottom of protrusion is less than the height that offset plate 7 to be dyed is inserted after cylinder body.The top of cylinder body 1 is that two ends are respectively protruded
5cm ± shapes, the bottom of protrusion less than the height 2cm that offset plate 7 to be dyed is inserted after cylinder body ±, so that testing crew is aobvious in silver staining
Color takes out offset plate after terminating.
The vertical neck 2 is evenly distributed on the relatively narrow side wall of cylinder body 1, and for the ease of operation, the quantity of neck 2 is
6-10 pairs；The bottom of neck 2 with cylinder base without being connected, so that decoction is in the circulation of whole cylinder body；The width of neck 2 should be bigger
In the thickness of offset plate 7 to be dyed, so as to which offset plate to be dyed is inserted in the mutually corresponding neck 2 of cylinder body 1.
Preferably, in this embodiment, between neck 2 at intervals of 1cm, the quantity of neck 2 is 8 pairs.The row
Fluid apertures 3 is located at cylinder body broad side walls bottom, is provided with the draining switch 4 whether control decoction flows out, and be connected with discharging tube
5, missed out so as to the safety nothing of various decoctions.The dyeing lid 6 for being arranged on the top of cylinder body 1 is in order to avoid various decoctions
Volatilization or AgNO3See light decompose.
Below to the operation using above-mentioned utility model device to the progress silver staining colour developing simultaneously of polylith polyacrylamide gel
Flow is briefly described.
(1) after polyacrylamide gel electrophoresis terminates, the polylith offset plate with gel is inserted perpendicularly into cylinder body 1 successively
Neck 2 in；
(2) close draining switch 4, toward cylinder body 1 in pour into enough fixers, cover after dyeing lid 6, fixed about 20min, it is loose
Draining switch 4 is opened, fixer is released through outage 3；
(3) close draining switch 4, toward cylinder body 1 in pour into enough rinsing liquids, cover after dyeing lid 6, rinsing about 2min, it is loose
Draining switch 4 is opened, rinsing liquid is released through outage 3；
(4) close draining switch 4, toward cylinder body 1 in pour into enough dyeing liquors, cover after dyeing lid 6, dyeing about 30min, it is loose
Draining switch 4 is opened, dyeing liquor is released through outage 3；
(5) close draining switch 4, toward cylinder body 1 in pour into enough distilled water, instantaneously get express developed with except removal floating color, release
Draining switch 4, distilled water is released through outage 3；
(6) close draining switch 4, toward cylinder body 1 in pour into enough developer solutions, cover dyeing lid 6, develop about 10min, release
Draining switch 4, developer solution is released through outage 3；
(7) be again switched off draining switch 4, toward cylinder body 1 in pour into enough distilled water, by the decoction soaking flushing on offset plate surface
Totally, draining switch 4 is unclamped, distilled water is released through outage 3；
(8) finally the polylith offset plate with gel is taken out from cylinder body 1 successively.
Embodiment of above is carried out in fume hood, to avoid murder by poisoning of the volatility toxic gas to testing crew, together
When be easy to the lower flume that waste liquid is directly discharged into through discharging tube in fume hood.
It is obvious to a person skilled in the art that will appreciate that above-mentioned specific embodiment is of the present utility model preferred
Scheme, therefore improvement, the variation that those skilled in the art may make to some of the utility model part, embodiment
It is still principle of the present utility model, realization is still the purpose of this utility model, belongs to the scope that the utility model is protected.
Claims (3)
1. a kind of polyacrylamide gel silver staining display device, it is characterised in that including open-topped cylinder body (1), the cylinder
It is provided with some to vertical neck (2) on the narrow side wall of body (1), the bottom of cylinder body (1) broad side walls offers outage (3), row
Fluid apertures (3) is provided with draining switch (4)；Outage (3) protrusion is arranged at outside the broad side walls of cylinder body (1)；The outage
(3) discharging tube (5) is connected with；The both upper ends thereof protrusion of the cylinder body (1), and the bottom of protrusion is less than offset plate to be dyed (7)
The height inserted after cylinder body.
2. polyacrylamide gel silver staining display device according to claim 1, it is characterised in that the vertical neck
(2) it is uniformly distributed on the narrow side wall of cylinder body (1), and thickness of the width more than offset plate to be dyed (7) of vertical neck (2), phase
Interval is provided between adjacent vertical neck (2).
3. polyacrylamide gel silver staining display device according to claim 1, it is characterised in that the cylinder body (1)
Top is provided with dyeing lid (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720009245.XU CN206531706U (en) | 2017-01-05 | 2017-01-05 | A kind of polyacrylamide gel silver staining display device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720009245.XU CN206531706U (en) | 2017-01-05 | 2017-01-05 | A kind of polyacrylamide gel silver staining display device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206531706U true CN206531706U (en) | 2017-09-29 |
Family
ID=59917611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720009245.XU Expired - Fee Related CN206531706U (en) | 2017-01-05 | 2017-01-05 | A kind of polyacrylamide gel silver staining display device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206531706U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286022A (en) * | 2019-05-27 | 2019-09-27 | 郑州中普医疗器械有限公司 | Fast constant temperature high definition dyes structural unit and high definition colouring method |
-
2017
- 2017-01-05 CN CN201720009245.XU patent/CN206531706U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110286022A (en) * | 2019-05-27 | 2019-09-27 | 郑州中普医疗器械有限公司 | Fast constant temperature high definition dyes structural unit and high definition colouring method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203929467U (en) | Running gel dyeing, decolouring and mobile device | |
| CN105571925A (en) | Dyeing module applied to biological samples on glass slides and dyeing method thereof | |
| CN206531706U (en) | A kind of polyacrylamide gel silver staining display device | |
| CN103196732A (en) | Pathological automatic DNA (deoxyribonucleic acid) staining system and staining method | |
| CN214427264U (en) | Automatic sulfide analyzer | |
| CN207133158U (en) | Heavy metal contaminants spread monitoring device in solidification and stabilization contaminated soil/solid waste | |
| CN205607717U (en) | Mineral products leaching device | |
| CN211061526U (en) | Water quality detection analyzer with digestion mechanism | |
| CN207656640U (en) | A kind of Jim Sa or Wright's staining slide irrigator stand | |
| CN202631338U (en) | Glass slide dye vat | |
| CN216525375U (en) | Device for chemical cleaning test | |
| CN205844063U (en) | A kind of gravity flow glass slide dyeing groove | |
| CN207263574U (en) | A kind of new Ubbelohde viscometer | |
| CN207280836U (en) | Film development box for immunohistochemical experiment | |
| CN205851475U (en) | A kind of special infuser device of laboratory color comparison tube | |
| CN108760449A (en) | The double-deck running gel dyeing apparatus, automation running gel dyeing apparatus and application method | |
| CN218012867U (en) | Laboratory plug scale test tube fixing device | |
| CN210803434U (en) | Device for measuring primary productivity of water body by black and white bottle method | |
| CN215179499U (en) | Soaking test device adopting free permeation method | |
| CN214133048U (en) | Water alkali wash tank | |
| CN217425114U (en) | Experimental device for be used for capillary action area research | |
| CN204122137U (en) | A kind of combined type lifting acid cylinder | |
| CN205308357U (en) | Sealing cylinder is soaked to function division formula lotion | |
| CN220912940U (en) | Movable salt fog test machine with anti-collision structure | |
| CN211070147U (en) | Water bath device for coconut oil temperature control in subareas |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170929 Termination date: 20190105 |