CN203849255U - Dynamic exposure device for daphnia detection - Google Patents
Dynamic exposure device for daphnia detection Download PDFInfo
- Publication number
- CN203849255U CN203849255U CN201320849692.8U CN201320849692U CN203849255U CN 203849255 U CN203849255 U CN 203849255U CN 201320849692 U CN201320849692 U CN 201320849692U CN 203849255 U CN203849255 U CN 203849255U
- Authority
- CN
- China
- Prior art keywords
- passage
- substrate
- magna
- chamber
- ring seal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
Abstract
The utility model relates to a dynamic exposure device for daphnia detection. The dynamic exposure device comprises a support, a sealing ring fixed on the upper end of the support, an observation vessel arranged below the sealing ring and a lifting platform for placing and fixing the observation vessel, wherein the sealing ring can seal an opening formed in the upper end of the observation vessel and is provided with a water sample in-out and balancing device, so that the dynamic exposure of the daphnia in a water sample is realized. The dynamic exposure device has the beneficial effects that the structure is simple, the long-term dynamic exposure of the daphnia in the water sample is realized, the foundation equipment is provided for a laboratory dynamic toxicity test and environment in-situ real-time on-line monitoring; the dynamic exposure device is used in match with a lighting device and a photographic device so as to be convenient to obtain the collection and analysis of the daphnia movement behavior video, and the environment can be accurately detected in real time.
Description
Technical field
The utility model relates to a kind of Magna detection dynamic exposing device.
Background technology
Daphnia magna (Daphnia magna) belongs to Arthropoda, Crustachia, and Branchiopoda, moves in natural water area, belongs to flotation crustaceans animal.It has that life cycle is short, breeding is fast, economical, be conveniently easy to get, responsive and be easy in advantages such as laboratory cultures to poisonous substance, add their importance in aquatic ecosystem, thereby be all applied in many countries, become a kind of test with model organism, be widely used in Ecology toxicological test.Daphnia magna toxicological test not only can be evaluated the pollution of sediment to water environment in industrial waste water, agricultural chemicals, chemicals and water, provides scientific basis for formulating various water quality standard, and can monitor as monitoring means the pollution of water environment.Utilize the experimental technique of Daphnia magna to be extensively subject to domestic and international many personages' attention, there is technical prospect widely.
Daphnia magna toxicity test adopts static exposure chamber to carry out conventionally, lack the online facility that dynamically exposes of laboratory and environment original position, and the use that dynamically exposes facility can be carried out experimental test more accurately, and be the prerequisite of real time on-line monitoring, therefore, the development and application of Aquatic Ecotoxicology dynamically exposes facility in the urgent need to corresponding Daphnia magna, and so dynamic exposure technology is combined with computer video and image processing techniques also can realize the robotization that Daphnia magna toxicity is tested.
Utility model content
The technical problems to be solved in the utility model is: based on the problems referred to above, provide a kind of Magna detection dynamic exposing device.
The utility model solves the technical scheme that its technical matters adopts: a kind of Magna detection dynamic exposing device, comprise support, be fixed on the O-ring seal of support upper end, the observation ware that is installed on O-ring seal below and the lifting platform of placement and fixed observer ware, O-ring seal is by the upper end open sealing of observation ware, and O-ring seal is provided with water sample turnover and balance device.
Further, inside observation ware, the back side is provided with the first substrate, in observation ware, be provided with right baffle-plate and right baffle plate, ware inner chamber is separated into left passage, middle passage and right passage by right baffle-plate and right baffle plate, the second substrate that middle passage upper end is provided with and is divided into cushion chamber, exposes chamber, the upper surface of the second substrate and O-ring seal laminating, the first substrate and the second substrate are structure as a whole; Expose bottom, chamber and be provided with the barrier for blocking Magna, right baffle-plate lower end offers the discharge orifice of passage in connection, left passage, and middle in-channel water sample enters left passage from discharge orifice through barrier; The second substrate middle part offers the water inlet through hole that is communicated with cushion chamber and exposes chamber, and the second substrate right side opening is provided with the spilling water passage that is communicated with cushion chamber and right passage.
Further, water sample turnover comprises the apopore being positioned at above left passage, the cushion chamber inlet opening, top that is positioned at apopore homonymy and the spillway hole that is positioned at right passage top with balance device.
Further, O-ring seal middle part offer with the through hole of intaking adapt for inserting the jack of temperature sensor, temperature sensor through jack, water inlet through hole, injects and exposes chamber successively.
Further, observation ware material is glass, and O-ring seal material is silicon rubber.
Further, the material of the first substrate, the second substrate, right baffle-plate and right baffle plate is non-toxic plastic.
The beneficial effects of the utility model are: simple in structure, can realize the long-term dynamics of Magna in water sample exposes, for the dynamic toxicological test in laboratory and environment original position real time on-line monitoring provide infrastructure device, it and lighting device, camera head are used in conjunction with, be convenient to obtain collection and the analysis of Magna motor behavior video, real-time testing environment more accurately.
Brief description of the drawings
Below in conjunction with accompanying drawing, the utility model is further illustrated.
Fig. 1 is structural representation of the present utility model;
Fig. 2 is vertical view of the present utility model;
Fig. 3 is the vertical view of observation ware;
Fig. 4 be in Fig. 3 A-A to cut-open view;
Fig. 5 be in Fig. 3 B-B to cut-open view;
Fig. 6 be in Fig. 3 C-C to cut-open view.
Wherein: 1. support, 2. O-ring seal, 3. observation ware, 4. the first substrate, 5. right baffle-plate, 6. right baffle plate, 7. cushion chamber, 8. expose chamber, 9. the second substrate, 10. barrier, 11. water inlet through holes, 12. spilling water passages, 13. apopores, 14. inlet openings, 15. spillway holes, 16. temperature sensors, 17. jacks, 18. discharge orifices, 19. lifting platforms.
Embodiment
By reference to the accompanying drawings the utility model is further described now.The schematic diagram that these accompanying drawings are simplification only illustrates basic structure of the present utility model in a schematic way, and therefore it only shows the formation relevant with the utility model.
A kind of Magna detection dynamic exposing device as shown in Fig. 1～6, comprise support 1, be fixed on the lifting platform 19 of the O-ring seal 2 of support 1 upper end, the observation ware 3 that is installed on O-ring seal 2 belows and placement and fixed observer ware 3, the upper end open sealing that O-ring seal 2 will observation ware 3.Observation ware 3 materials are glass, and O-ring seal 2 materials are silicon rubber.
Observation ware 3 back sides, inner side are provided with the first substrate 4, in observation ware 3, be provided with right baffle-plate 5 and right baffle plate 6, observation ware 3 inner chambers are separated into left passage, middle passage and right passage by right baffle-plate 5 and right baffle plate 6, the second substrate 9 that middle passage upper end is provided with and is divided into cushion chamber 7, exposes chamber 8, the upper surface of the second substrate 9 and O-ring seal 2 are fitted, and the first substrate 4 and the second substrate 9 are structure as a whole.The material of the first substrate 4, the second substrate 9, right baffle-plate 5 and right baffle plate 6 is non-toxic plastic.
Expose 8 bottoms, chamber and be provided with the barrier 10 for blocking Magna, right baffle-plate 5 lower ends offer the discharge orifice 18 of passage in connection, left passage, and middle in-channel water sample enters left passage through barrier 10 from discharge orifice 18.
The second substrate 9 middle parts offer water inlet through hole 11, the second substrate 9 right side openings that are communicated with cushion chamber 7 and expose chamber 8 and are provided with the spilling water passage 12 that is communicated with cushion chamber 7 and right passage.
O-ring seal 2 is provided with water sample turnover and balance device.Water sample turnover and balance device comprise be positioned at left passage top apopore 13, be positioned at apopore 13 homonymies cushion chamber 7 tops inlet opening 14 and be positioned at the spillway hole 15 of right passage top.O-ring seal 2 middle parts offer with the through hole 11 of intaking adapt for inserting the jack 17 of temperature sensor 16.Temperature sensor 16, for measuring the Exposure Temperature of Magna at water sample, is convenient to maintain the isoperibol that exposes chamber 8, reduces the impact of temperature variation on Magna toxotest.
When actual use, the liquid that Magna is housed is packed into and exposed in chamber 8 from water inlet through hole 11.Expose between four spaces such as chamber 8, left passage, cushion chamber 7 and right passage and have discharge orifice 18, water inlet through hole 11, spilling water passage 12 and observation ware 3 glass inwalls to be communicated with the trickle gap between plastic baffle plate, add liquid to liquid level and flush with cushion chamber 7 lower surface.Now, Magna, exposing the interior activity in chamber 8, exposes in chamber 8 and does not stay bubble.
Observation ware 3 is placed on lifting platform 19, and lifting platform 19 rises fixing, observation ware 3 and O-ring seal 2 gluing, sealings.Apopore 13, inlet opening 14, spillway hole 15 connect respectively rising pipe (rising pipe lower end will be deep into left channel bottom), water inlet pipe, overflow pipe, the interior insertion temperature sensor 16 of jack 17, temperature sensor 16 through jack 17, water inlet through hole 11, injects and exposes chamber 8 successively.Because jack 17 internal diameters are smaller compared with temperature sensor 16 external diameters, water inlet through hole 11 internal diameters are bigger compared with temperature sensor 16 external diameters, jack 17 is by fixing temperature sensor 16, and the water in cushion chamber 7 can enter and expose chamber 8 from the gap between water inlet through hole 11 and temperature sensor 16.
Inlet opening 14 water flowings, control the interior water flow in inlet opening 14, will in cushion chamber 7, be full of, and the water in cushion chamber 7 can enter and expose chamber 8 from the gap between water inlet through hole 11 and temperature sensor 16.Magna contacts exposure in exposure chamber 8 with water sample, current continue to enter left passage by barrier 10 through discharge orifice 18, is then discharged through apopore 13 by rising pipe bottom, forms water sample turnover control, realizes the dynamic exposure that exposes chamber 8 interior Magna.
The flow of controlling inlet opening 14 is greater than the flow of apopore 13 all the time slightly, keep this small pressure reduction, unnecessary water sample is discharged through spillway hole 15 from spilling water passage 12, thereby, realize control and the balance of water sample turnover, ensureing to expose chamber 8 has the dynamic water sample of constant volume and the normal operation that Magna dynamically exposes all the time.
Because inlet opening 14 is positioned at left side, the air in water inlet pipe and cushion chamber 7 can be rushed to by current the spilling water passage 12 on right side, arrives right passage, discharges from spillway hole 15, ensures the liquid environment of whole observation, does not have bubble the dynamic exposure of Magna is caused to interference.
Taking above-mentioned foundation desirable embodiment of the present utility model as enlightenment, by above-mentioned description, relevant staff can, not departing from the scope of this utility model technological thought, carry out various change and amendment completely.The technical scope of this utility model is not limited to the content on instructions, must determine its technical scope according to claim scope.
Claims (6)
1. a Magna detection dynamic exposing device, it is characterized in that: comprise support (1), be fixed on the O-ring seal (2) of support (1) upper end, the observation ware (3) that is installed on O-ring seal (2) below and the lifting platform (19) of placement and fixed observer ware (3), O-ring seal (2) will be observed the upper end open sealing of ware (3), and O-ring seal (2) is provided with water sample turnover and balance device.
2. Magna detection dynamic exposing device according to claim 1, it is characterized in that: the described back side, observation ware (3) inner side is provided with the first substrate (4), in observation ware (3), be provided with right baffle-plate (5) and right baffle plate (6), right baffle-plate (5) and right baffle plate (6) will observe ware (3) inner chamber be separated into left passage, middle passage and right passage, middle passage upper end is provided with and is divided into cushion chamber (7), expose second substrate (9) in chamber (8), the upper surface of the second substrate (9) and O-ring seal (2) laminating, the first substrate (4) is structure as a whole with the second substrate (9),
Expose bottom, chamber (8) and be provided with the barrier (10) for blocking Magna, right baffle-plate (5) lower end offers the discharge orifice (18) of passage in connection, left passage, and through barrier (10), from discharge orifice, (18) enter left passage to middle in-channel water sample;
In the middle part of the second substrate (9), offer and be communicated with cushion chamber (7) and the water inlet through hole (11) that exposes chamber (8), the second substrate (9) right side opening is provided with the spilling water passage (12) that is communicated with cushion chamber (7) and right passage.
3. Magna detection dynamic exposing device according to claim 1, is characterized in that: the turnover of described water sample and balance device comprise be positioned at left passage top apopore (13), be positioned at apopore (13) homonymy cushion chamber (7) top inlet opening (14) and be positioned at the spillway hole (15) above right passage.
4. Magna detection dynamic exposing device according to claim 1, it is characterized in that: described O-ring seal (2) middle part offer with the through hole of intaking (11) adapt for inserting the jack (17) of temperature sensor (16), temperature sensor (16), successively through jack (17), water inlet through hole (11), injects and exposes chamber (8).
5. Magna detection dynamic exposing device according to claim 1, is characterized in that: described observation ware (3) material is glass, and O-ring seal (2) material is silicon rubber.
6. Magna detection dynamic exposing device according to claim 2, is characterized in that: the material of described the first substrate (4), the second substrate (9), right baffle-plate (5) and right baffle plate (6) is non-toxic plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320849692.8U CN203849255U (en) | 2013-12-20 | 2013-12-20 | Dynamic exposure device for daphnia detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320849692.8U CN203849255U (en) | 2013-12-20 | 2013-12-20 | Dynamic exposure device for daphnia detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203849255U true CN203849255U (en) | 2014-09-24 |
Family
ID=51562256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320849692.8U Expired - Fee Related CN203849255U (en) | 2013-12-20 | 2013-12-20 | Dynamic exposure device for daphnia detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203849255U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103743877A (en) * | 2013-12-20 | 2014-04-23 | 常州市环境监测中心 | Dynamic exposure device for daphnia detection |
| CN113311144A (en) * | 2021-05-20 | 2021-08-27 | 江南大学 | Method for evaluating toxicity of micro-plastic based on behavioral indexes of daphnia magna |
-
2013
- 2013-12-20 CN CN201320849692.8U patent/CN203849255U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103743877A (en) * | 2013-12-20 | 2014-04-23 | 常州市环境监测中心 | Dynamic exposure device for daphnia detection |
| CN103743877B (en) * | 2013-12-20 | 2015-09-16 | 常州市环境监测中心 | Magna detection dynamic exposing device |
| CN113311144A (en) * | 2021-05-20 | 2021-08-27 | 江南大学 | Method for evaluating toxicity of micro-plastic based on behavioral indexes of daphnia magna |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102937637B (en) | Water body experiment apparatus and method | |
| CN104880457B (en) | A kind of contactless pollutant concentration diffusion detecting system and method | |
| CN110596200B (en) | Underground water stratified sampling detection device and detection method | |
| CN107917947A (en) | A kind of online water quality detecting device | |
| CN203849255U (en) | Dynamic exposure device for daphnia detection | |
| CN104020097A (en) | Indoor measurement experiment instrument of hydraulic conductivity of unsaturated soil | |
| CN206450467U (en) | A kind of water quality monitoring pretreatment unit and water quality early-warning device | |
| CN109187646A (en) | A kind of microbubble and wadding body comprehensive detection system and method | |
| CN207717441U (en) | A kind of water environment sampling apparatus | |
| CN103743877B (en) | Magna detection dynamic exposing device | |
| CN108272455B (en) | Real-time oxygen consumption monitoring system for small animals with modularized water-seal anoxic bottles | |
| CN108742632B (en) | Real-time oxygen consumption monitoring system for small animals with hypoxic anoxic bottles | |
| CN114184757B (en) | Method for measuring denitrification rate of suspended matters in water body | |
| CN103105425B (en) | Water quality detection device and method | |
| CN110095424A (en) | A kind of four parameter online integrated apparatus of black and odorous water | |
| CN206583897U (en) | A kind of chlorine residue detects flow cell | |
| CN115508043A (en) | Landslide accumulation body seepage hysteresis nature analogue test device | |
| CN212206762U (en) | Device for collecting water sample in river | |
| CN108514417B (en) | Respiration oxygen consumption real-time monitoring device with coiling oxygen consumption monitoring groove | |
| CN104777204B (en) | Integrated form screen printing electrode detection handle with agitating function | |
| CN108514416B (en) | Respiration oxygen consumption real-time monitoring device with upper drainage structure | |
| CN216350365U (en) | Algae detection inlet and outlet sample mechanism | |
| CN201156034Y (en) | Water quality measuring instrument | |
| CN206740711U (en) | A kind of new device for determining water body diffused methane release flux | |
| KR100990368B1 (en) | Volume measuring device for gas produced by reaction of micro-organism in reaction tank and multi channel type automatic gas analyzing system comprising the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140924 Termination date: 20151220 |
|
| EXPY | Termination of patent right or utility model |