CN108514416B - A real-time monitoring device for respiratory oxygen consumption with upper drainage structure - Google Patents
A real-time monitoring device for respiratory oxygen consumption with upper drainage structure Download PDFInfo
- Publication number
- CN108514416B CN108514416B CN201810149643.0A CN201810149643A CN108514416B CN 108514416 B CN108514416 B CN 108514416B CN 201810149643 A CN201810149643 A CN 201810149643A CN 108514416 B CN108514416 B CN 108514416B
- Authority
- CN
- China
- Prior art keywords
- groove
- oxygen consumption
- consumption monitoring
- drainage
- monitoring
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Measuring devices for evaluating the respiratory organs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/08—Measuring devices for evaluating the respiratory organs
- A61B5/083—Measuring rate of metabolism by using breath test, e.g. measuring rate of oxygen consumption
- A61B5/0833—Measuring rate of oxygen consumption
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Pulmonology (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Physiology (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Emergency Medicine (AREA)
- Obesity (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
一种带有带有上排水结构的呼吸耗氧量实时监测装置，本体内镂刻有容量槽、耗氧监测槽、进气引导槽、排水导槽，本体的上表面上设有进气口和出气口；进气口通过进气引导槽与容量槽连通；耗氧监测槽的一端连通容量槽，另一端连通出气口；容量槽与耗氧监测槽的连接处设有凸起；耗氧监测槽的槽底上均匀地设有若干个光敏电阻；光敏电阻依次串联形成采样电阻，并通过电阻检测电路连接生物信号采集处理系统；耗氧监测槽具有盘曲的形状；排水导槽的出口均通过回水导槽连通容量槽和进气引导槽；耗氧监测槽与每个排水导槽的进口之间通过防倒流的导流装置连通，排水凹坑与导流块之间具有连通耗氧监测槽和排水导槽的间隙。
A real-time monitoring device for respiratory oxygen consumption with an upper drainage structure, the body is engraved with a capacity groove, an oxygen consumption monitoring groove, an air intake guide groove, and a drainage guide groove, and the upper surface of the body is provided with an air inlet and a Air outlet; the air inlet is communicated with the capacity groove through the air intake guide groove; one end of the oxygen consumption monitoring groove is connected to the capacity groove, and the other end is connected to the air outlet; the connection between the capacity groove and the oxygen consumption monitoring groove is provided with a protrusion; the oxygen consumption monitoring groove Several photoresistors are evenly arranged on the bottom of the tank; the photoresistors are connected in series to form sampling resistors, and are connected to the biological signal acquisition and processing system through the resistance detection circuit; the oxygen consumption monitoring tank has a coiled shape; the outlet of the drainage channel passes through The return water channel communicates with the capacity channel and the intake channel; the oxygen consumption monitoring channel and the inlet of each drainage channel are communicated with the anti-backflow diversion device, and there is a communication between the drainage pit and the diversion block for oxygen consumption monitoring Gutter and drainage channel clearance.
Description
Technical Field
The invention relates to a respiratory oxygen consumption real-time monitoring device with an upper drainage structure.
Background
Hypoxia is an important pathogenic factor or one of important pathological processes in the process of the onset of various diseases of the body, and is also a problem which needs to be researched in plateau life, mine tunnel operation and aerospace flight. Various hypoxia experimental models are commonly used in experiments of higher medical colleges to simulate hypoxia, and the simulated hypoxia experimental models are used for research on physiological, biochemical, pharmacological, pathophysiological and clinical aspects, so as to explain the pathological process of various clinical diseases and explore effective treatment means of the diseases.
At present, in the teaching materials for medical experiment planning in universities and colleges in China, a closed system consisting of a 500ml wide-mouth bottle, a 25ml fat belly straw, a 50ml measuring cylinder and an oxygen-deficient bottle is commonly adopted as a classical device for non-anesthesia small animal oxygen-deficient experiments to detect the oxygen consumption of mice (see figure 1, in figure 1, a is the oxygen-deficient bottle, b is soda lime, c is the fat belly straw and d is the measuring cylinder), or a 125 plus-500 ml wide-mouth bottle is used for closing to directly detect the oxygen-deficient resistant time, and the respiratory frequency is counted by a visual method at a specified time point. As the mouse is used as an experimental object, the breathing frequency is fast (200 times/min-300 times/min), the body position is frequently rotated in the oxygen deficiency process, the breathing frequency of the mouse cannot be accurately counted on time by a visual method, so that the experimental result is often inconsistent with the expected result, and the experimental effect and the teaching quality are influenced.
In addition, in the conventional oxygen consumption device, the mouse breathes to consume oxygen, so that water in the measuring cylinder moves to the fat belly suction pipe, the pressure in the anoxic bottle is reduced, namely, the mouse is anoxic in a negative pressure environment, and the measurement result of the oxygen consumption generates systematic errors.
In recent years, although there are reports of mouse respiratory transduction amplifier products, mouse respiratory frequency measurement in a closed environment by using a biological signal acquisition and processing system and a common pressure transducer, a mouse normal-pressure acute hypoxia model device and a mouse oxygen consumption dynamic change measuring device, a quantitative analysis device which can realize normal pressure and constant pressure and realize real-time dynamic automatic tracing of oxygen consumption and a respiratory curve in a closed environment has not been reported.
Disclosure of Invention
In order to overcome the defects of the oxygen-deficiency device, the invention provides a respiratory oxygen consumption real-time monitoring device with an upper drainage structure.
The technical scheme for solving the problems is as follows:
a real-time respiratory oxygen consumption monitoring device with an upper drainage structure comprises a horizontally laid body, wherein the body is a colorless and transparent acrylic plate, and the lower surface of the body is tightly attached to a flat shading plate; a capacity groove, an oxygen consumption monitoring groove and an air inlet guide groove are engraved in the body, and an air inlet and an air outlet for communicating the anoxic bottle are arranged on the upper surface of the body; the air inlet is communicated with the capacity groove through an air inlet guide groove; one end of the oxygen consumption monitoring groove is communicated with the volumetric groove, and the other end of the oxygen consumption monitoring groove is communicated with the gas outlet; the air outlet is connected with the oxygen-deficient bottle through a high-sensitivity transducer for collecting respiratory motion data, and the high-sensitivity transducer is connected with a biological signal collecting and processing system;
a bulge is arranged at the joint of the capacity tank and the oxygen consumption monitoring tank, and the bulge shields part of the cross section of the inlet of the oxygen consumption monitoring tank;
scales are arranged on the upper surface of the body along the length direction of the oxygen consumption monitoring groove; a plurality of photoresistors are uniformly arranged on the bottom of the oxygen consumption monitoring groove along the length direction of the oxygen consumption monitoring groove; the photoresistors are sequentially connected in series to form a sampling resistor, the sampling resistor is connected to a resistor detection circuit, the output end of the resistor detection circuit is connected with a biological signal acquisition and processing system, and the biological signal acquisition and processing system outputs animal oxygen consumption data according to the data of the sampling resistor;
the oxygen consumption monitoring groove has a serpentine shape; the oxygen consumption monitoring groove comprises a plurality of sections of straight grooves which are parallel to each other and a curved groove which is connected with two adjacent straight grooves;
a plurality of drainage guide grooves and a backwater guide groove are engraved in the body, and outlets of the drainage guide grooves are communicated with the capacity groove and the air inlet guide groove through the backwater guide groove;
the oxygen consumption monitoring groove is communicated with the inlet of each drainage guide groove through a backflow-preventing guide device, each guide device comprises a guide block and a drainage pit covered on the guide block, and a gap communicated with the oxygen consumption monitoring groove and the drainage guide groove is formed between each drainage pit and the guide block;
the drainage pit is arranged on the body, and the flow guide blocks are respectively and fixedly arranged between each curved groove close to one side of the drainage guide groove and the drainage guide groove; defining one end of the flow guide block close to the curved groove as a left end and one end close to the drainage guide groove as a right end; the bottom surface of water conservancy diversion piece and the top surface looks parallel and level of oxygen consumption monitoring groove, the top surface of water conservancy diversion piece is the inclined plane that rises from the left end to the right end slope gradually, just the left end on inclined plane extends to curved groove department, the right-hand member on inclined plane extends to in the drainage guide slot.
Further, the groove depths of the capacity groove, the oxygen consumption monitoring groove, and the intake guide groove were 0.5 cm.
Further, air inlet and gas outlet all are located the upper left side of body, and the capacity trench is located the right side below of body.
Further, the concrete intercommunication structure of anoxic bottle and gas outlet is: one side of the body is provided with a conversion interface communicated with the gas outlet, the other side of the body is provided with an oxygen deficiency bottle external interface communicated with the oxygen deficiency bottle, and the conversion interface is communicated with the oxygen deficiency bottle external interface through a high-sensitivity transducer.
Further, the air outlet and the conversion interface are detachably connected through a connecting pipe.
Further, the straight groove horizontally extends from left to right on the body, and the straight groove is vertically connected with the bent groove.
Further, the flow guide block is a wedge-shaped block.
As the invention designs the flow guide blocks 21 and 22 and the drainage pits 23 and 24, the ink in the capacity tank 05 can be reused, and the specific operation is as follows: the body is erected, the oxygen consumption monitoring groove 15 faces upwards, the capacity groove 05 faces downwards, and water in the oxygen consumption monitoring groove 15 can flow into the drainage guide grooves 06 and 07 through gaps between the guide blocks 21 and 22 and the drainage pits 23 and 24 and sequentially flows into the capacity groove 05 through the drainage guide grooves 06 and 07 and the water return guide grooves for standby. And then the breathing oxygen consumption real-time monitoring device is horizontally placed on the desktop, so that the experiment can be carried out again, and the trouble that water needs to be added in each experiment is avoided.
The invention has the following beneficial effects:
1. in the experiment, the liquid column for measuring oxygen consumption is in a horizontal state, so that when the liquid column moves, the liquid column does not affect the pressure in the hypoxic oxygen bottle, the problem that when water in the measuring cylinder moves to the fat belly suction pipe due to oxygen consumption of mice in a conventional oxygen consumption device through respiration, the mice lack oxygen in a negative pressure environment, and accordingly the oxygen consumption measurement result generates systematic errors is solved, and the measurement precision is effectively improved.
2. The invention solves the problem that the respiration and oxygen consumption can not be automatically monitored by a common classical oxygen depletion device, adopts the principle that pressure fluctuation caused by photoelectric induction and thoracic contraction can be transmitted in a closed container, solves the problem that the respiration frequency and the oxygen consumption are counted by a manual visual method in a conventional oxygen depletion experiment, can realize automatic tracing of the oxygen depletion oxygen consumption in any time period and tracing of the respiration curves of different types of oxygen depletion by using a respiration oxygen depletion real-time monitoring device, realizes comparison of the respiration excitability of different types of oxygen depletion experimental animals by analyzing the respiration curves, lightens the labor intensity of the experiment and improves the accuracy of the experiment result.
3. Because the oxygen consumption monitoring groove has the shape of the spiral curve, the length of the oxygen consumption monitoring groove can be longer when the size of the body is limited; then, at a certain measuring range, the oxygen consumption monitoring groove can have a smaller cross section area, and the reaction of the liquid column is more sensitive, so that the oxygen consumption monitoring groove has more accurate measuring accuracy.
4. The invention is erected, the oxygen consumption monitoring groove faces upwards, the capacity groove faces downwards, water flowing into the oxygen consumption monitoring groove in the experiment can be discharged into the drainage guide groove through the gap between the flow guide block and the drainage pit and finally flows to the capacity groove, the drainage is very convenient, and the trouble that water needs to be added in each experiment is avoided. Meanwhile, the flow guide device can prevent the liquid in the volume groove from flowing to the volume groove when the body is horizontally placed.
Drawings
FIG. 1 is a schematic diagram of a classical apparatus for hypoxia experiments in non-anesthetized small animals;
FIG. 2 is a top view of a respiratory oxygen consumption real-time monitoring device;
FIG. 3 is an anatomical axis one of the internal lumens of the respiratory oxygen consumption real-time monitoring device;
FIG. 4 is an isometric view one of the respiratory oxygen consumption real-time monitoring device;
FIG. 5 is an isometric view of a respiratory oxygen consumption real-time monitoring device;
FIG. 6 shows the measurement results of "dynamic measurement of the whole oxygen consumption of mice" in Jiang Shi, xushuxiu, etc., according to the "Chinese pharmacological report";
fig. 7 is an automated trace of hypoxic oxygen consumption using the present invention.
In the figure: 01. the device comprises a power supply interface, 02, a power supply switch, 03, an oxygen consumption signal interface, 04, a breathing signal interface, 05, a capacity groove, 06, a drainage guide groove 1, 07, a drainage guide groove 2, 08, a protrusion, 09, a maintenance groove, 10-13, a maintenance groove, 14, an air inlet guide groove, 15, an oxygen consumption monitoring groove, 16, an oxygen deficiency bottle external interface, 17, a conversion interface, 18, an air outlet, 19, an air inlet, 20, a photosensitive resistor, 21-22 flow guide blocks and 23-24 drainage pits.
Detailed Description
Referring to the attached drawings, the device for monitoring the respiratory oxygen consumption in real time with the upper drainage structure comprises a horizontally laid body, wherein the body is a colorless and transparent acrylic plate, and the lower surface of the body is tightly attached to a flat light screen; a capacity groove 05, an oxygen consumption monitoring groove 15 and an air inlet guide groove 14 are engraved in the body, and an air inlet 19 and an air outlet 18 for communicating an oxygen-deficient bottle are arranged on the upper surface of the body; the intake port 19 communicates with the capacity tank 05 through the intake guide groove 14; one end of the oxygen consumption monitoring groove 15 is communicated with the capacity groove 05, and the other end is communicated with the air outlet 18; the air outlet 18 is connected with the oxygen-deficient bottle through a high-sensitivity transducer for collecting respiratory motion data, and the high-sensitivity transducer is connected with a biological signal collecting and processing system;
a bulge is arranged at the joint of the volumetric groove 05 and the oxygen consumption monitoring groove 15, and the bulge shields part of the cross section of the inlet of the oxygen consumption monitoring groove 15;
scales are arranged on the upper surface of the body along the length direction of the oxygen consumption monitoring groove 15; a plurality of photoresistors 20 are uniformly arranged on the bottom of the oxygen consumption monitoring groove 15 along the length direction of the oxygen consumption monitoring groove 15; the photoresistors 20 are sequentially connected in series to form a sampling resistor, the sampling resistor is connected to a resistor detection circuit, the output end of the resistor detection circuit is connected with a biological signal acquisition and processing system, and the biological signal acquisition and processing system outputs animal oxygen consumption data according to the data of the sampling resistor;
the oxygen consumption monitoring groove 15 has a serpentine shape; the oxygen consumption monitoring groove 15 comprises a plurality of sections of parallel straight grooves and a curved groove for connecting two adjacent straight grooves;
a plurality of drainage guide grooves 06, 07 and a backwater guide groove are engraved in the body, and outlets of the drainage guide grooves 06, 07 are communicated with the capacity groove 05 and the air inlet guide groove 14 through the backwater guide grooves;
the oxygen consumption monitoring groove 15 is communicated with the inlets of the drainage guide grooves 06 and 07 through backflow-proof guide devices respectively, each guide device comprises a guide block and drainage pits 23 and 24 covered on the guide block, and gaps communicated with the oxygen consumption monitoring groove 15 and the drainage guide grooves 06 and 07 are formed between the drainage pits 23 and 24 and the guide blocks 21 and 22;
The flow guide blocks 21 and 22 can block the liquid from the drainage guide grooves 06 and 07 from flowing to the oxygen consumption monitoring groove 15 when the body is horizontally placed, and can guide the liquid from the oxygen consumption monitoring groove 15 to flow into the drainage guide grooves 06 and 07 when the body is vertically placed.
The oxygen consumption monitoring tank 15 is suitably formed as an elongated tank of small cross section so as to exhibit good sensitivity to the respiration of the animal in the experiment, and even the respiration of the mouse can cause the liquid column of the ink to move significantly.
The groove depths of the capacity groove 05, the oxygen consumption monitoring groove 15, and the intake guide groove 14 are all 0.5 cm. After long-term repeated tests, the optimal choice is found that the depth of the oxygen consumption monitoring groove 15 is 0.5cm, and the width of the groove is 1cm, ink can flow in a layered mode when the groove depth is too high, and the end face of an ink liquid column can move in a non-parallel mode when the groove is too wide, so that the accurate recording of oxygen consumption is influenced; too shallow and too narrow grooves can result in too long lumen, making the device too bulky.
The air inlet 19 and the air outlet 18 are both located at the upper left of the body, and the capacity groove 05 is located at the lower right of the body.
The oxygen-deficient bottle and the concrete communicating structure of the gas outlet are as follows: one side of the body is provided with a conversion interface 17 communicated with the air outlet 18, the other side of the body is provided with an oxygen deficiency bottle external interface 16 communicated with the oxygen deficiency bottle, and the conversion interface 17 is communicated with the oxygen deficiency bottle external interface 16 through a high-sensitivity transducer.
The air outlet 18 and the conversion interface 17 are detachably connected through a connecting pipe, and the connecting pipe is a silicone tube.
The straight groove horizontally extends from left to right on the body, and the straight groove is vertically connected with the bent groove.
The guide blocks 21 and 22 are wedge-shaped blocks.
The light shielding plate is flatly laid on the base, a power supply interface 01 is arranged on the outer side surface of the base, the photosensitive resistor 20 is communicated with an external power supply through the power supply interface 01, and the power supply interface 01 is provided with a power supply switch 02; an oxygen consumption signal interface 03 is arranged on the outer side surface of the base, and the photoresistor 20 is connected with a biological signal acquisition and processing system through the oxygen consumption signal interface 03;
still be equipped with the high sensitive tension transducer who is used for communicateing the hypoxia chamber in the base, high sensitive tension transducer passes through respiratory signal interface 04 and links to each other with biological signal acquisition processing system, and respiratory signal interface 04 is located the lateral surface of base.
The oxygen consumption monitoring groove 15 has a width of 1cm and a groove depth of 0.5cm, and has a capacity of 45 to 55ml of water in total.
The volume groove 05, the oxygen consumption monitoring groove 15, the air inlet guide groove 14 and the water drainage guide grooves 06 and 07 engraved in the body can be engraved in the body or on the lower surface of the body. When the air inlet guide groove is carved in the body, the groove bottoms of the capacity groove 05, the oxygen consumption monitoring groove 15, the air inlet guide groove 14 and the water drainage guide grooves 06 and 07 are required to be positioned on the same horizontal plane; when the light shielding plates are engraved on the lower surface of the body, the light shielding plates serve as the groove bottoms of the grooves.
When the present invention is placed horizontally and no external force is applied, the ink having a thickness of 0.5cm in the capacity tank 05 does not flow under the surface tension and the adhesion, so that the water in the capacity tank 05 does not flow into the oxygen consumption monitoring tank 15 before the start of the experiment. Meanwhile, the protrusion shields a part of the cross section of the inlet of the oxygen consumption monitoring groove 15, so that the flow resistance of the ink at the inlet of the oxygen consumption monitoring groove 15 is increased, and the ink in the volume groove 05 cannot flow into the oxygen consumption monitoring groove 15 before the experiment starts.
After the experiment is started, the ink flows into the oxygen consumption monitoring groove 15 by overcoming the resistance at the inlet of the oxygen consumption monitoring groove 15 under the traction of the negative pressure of the anoxic bottle, and flows to the air outlet 18 along the oxygen consumption monitoring groove 15. At this time, the ink in the volume tank 5 decreases, and the gas inlet 19 supplies gas to the volume tank 5 through the gas inlet guide groove 14.
During the experiment (the body is horizontally arranged), when the ink flows through the bent groove of the oxygen consumption monitoring groove 15, the ink meets the flow guide device, and the flow guide blocks 21 and 22 can prevent the liquid medium in the capacity groove 05 from flowing back into the oxygen consumption monitoring groove 15. The plane of the bottom openings of the drainage pits 23 and 24 is flush with the top surface of the oxygen consumption monitoring groove 15, and under the action of gravity, the liquid medium in the oxygen consumption monitoring groove 15 cannot fill the gaps between the drainage pits 23 and 24 and the flow guide blocks 21 and 22, so that the flow guide device cannot influence the oxygen consumption measurement result.
The length of the ink liquid column reflects the oxygen consumption of the experimental animal, the resistance value of the sampling resistor changes when the photosensitive resistor 20 is submerged by the ink liquid column, and the photosensitive resistors 20 which are regularly arranged under the oxygen consumption monitoring groove 15 can be used for automatically sensing the oxygen consumption. The principle is that natural light or lamplight is used as an emitting end, the photoresistor is used as a receiving end, and the resistance value of the photoresistor 20 submerged by the ink liquid column in the oxygen consumption monitoring groove 15 changes by utilizing the characteristics that the photoresistor is low in resistance value when light is strong and is high in resistance value when light is dark. The light signals are converted into electric signals by using a resistance detection circuit in a bridge form, a trend curve of oxygen consumption is displayed by biological signal acquisition and processing system software, and the detection signals are digitized.
1. The method comprises the following steps:
1) the invention is erected with the capacity tank 05 facing downwards and the oxygen consumption monitoring tank 15 facing upwards; the silicon rubber tube is used for communicating the air outlet 18 and the conversion interface 17; then, black ink diluted with distilled water is added into the volume tank 05 from the inlet port 19 until the water level rises to the 0 th mark of the oxygen consumption monitoring tank 15 (i.e., the inlet of the oxygen consumption monitoring tank 15).
2) The invention is horizontally placed on a table top, and the respiratory signal interface 04 is connected with a corresponding channel of a biological signal acquisition and processing system; then, the oxygen consumption signal interface 03 is connected with a corresponding channel of the biological signal acquisition and processing system; and finally, connecting the power interface 01 with a power supply.
3) Putting soda lime and a mouse into an anoxic bottle, and covering a sealing cover; then the vent pipe on the sealing cover and communicated with the oxygen-poor bottle is communicated through an external connector 16 of the oxygen-poor bottle.
4) The power switch 02 is activated and the experiment begins. When the mouse in the anoxic bottle breathes and consumes oxygen to generate negative pressure, such as 0.5cmH2The ink in the O column and the volume groove 05 can flow towards the oxygen consumption monitoring groove 15 to fill up the consumed oxygen volume due to the action of negative pressure, so that the pressure in the invention is always constant at-0.5 cm H2And (4) an O column. The carbon dioxide exhaled by the mouse is absorbed by the soda lime placed in the oxygen-depleted bottle, so that the milliliter number of the ink flowing from the capacity groove 05 to the oxygen consumption monitoring groove 15 is the oxygen consumption of the mouse (the oxygen consumption can be read through scales on the surface of the oxygen consumption monitoring groove 15 by manual monitoring);
when the diluted ink in the capacity tank 05 flows to the oxygen consumption monitoring tank 15, the photoresistor 20 can be submerged, the light which originally shines on the photoresistor 20 is shielded, the more ink flows into the oxygen consumption monitoring tank 15, the more the photoresistor 20 is shielded, the effective signal can be filtered out by the resistance detection circuit and the voltage output can be controlled by applying the signal, and the oxygen consumption can be automatically traced by the biological signal acquisition and processing system.
When the mouse breathes, the expansion and contraction of the lung cause pressure fluctuation in the breathing oxygen consumption real-time monitoring device, the pressure fluctuation is converted into an electric signal through the high-sensitivity transducer and transmitted to the RM6240 biological signal acquisition and processing system, and a breathing curve is traced by using a direct current signal, so that the pressure, the breathing frequency and the breathing amplitude in the breathing oxygen consumption real-time monitoring device can be accurately and dynamically monitored, the breathing frequency, the breathing amplitude and the pressure of the mouse in an anoxia experiment can be automatically monitored, and errors caused by manual monitoring can be avoided.
5) To conduct the experiment again, the present invention is erected with the oxygen consumption monitoring groove 15 facing upward and the capacity groove 05 facing downward, and the water in the oxygen consumption monitoring groove 15 flows into the capacity groove 05 through the gaps between the drain recesses 23, 24 and the drain guide grooves 06, 07. After water flowing into the oxygen consumption monitoring groove 15 in the draining experiment, the breathing oxygen consumption real-time monitoring device is horizontally placed on a desktop, so that the experiment can be carried out again, and the trouble that water needs to be added in the experiment at every time is avoided.
2. Experimental results and Explanation of the invention
According to the results of 'dynamic measurement of the whole oxygen consumption of mice' of Jiang Shi, xushuxiu and the like (see figure 6) and the measurement results of the oxygen consumption by using the invention (see figure 7), the method can obtain the following results: the oxygen consumption bottles used in different laboratory oxygen consumption experiments have different capacities, the oxygen consumption survival time is obviously different, the oxygen consumption curves are all a bidirectional curve which is firstly increased and then decreased, the oxygen consumption mainly occurs in the first half period, for example, the oxygen consumption accounts for 71 percent of the total oxygen consumption in the first 12 minutes and 21 percent of the total oxygen consumption in the second half period in the oxygen consumption measurement process by utilizing the oxygen consumption measuring device, and the oxygen consumption is decreased at a constant speed; indicating that the first half of the measurement yielded a significant change in oxygen consumption (i.e., a significant difference was obtained).
The invention can collect the respiratory frequency, amplitude (ventilation) and oxygen consumption in any time period in the process of oxygen deficiency by recording the respiratory curve by the biological signal collecting and processing system, overcomes the problem that the prior device counts the respiratory frequency (the mouse breathes weakly and quickly and the respiration frequency is more than 200 times/min, and the data can not be obtained accurately on time, and even if the respiratory frequency data is obtained, the problem that the respiratory frequency and the respiratory excitation are not necessarily in positive correlation can not be corrected), and can change the conventional method of using the limited oxygen for oxygen deficiency by popularizing and using the invention, change the method of using the limited oxygen for time consumption rate or minute oxygen consumption to obtain the experimental result, and avoid the harm of the limited oxygen to animals.
On the basis of classical characteristics, the invention breaks through the recording mode of the existing anoxic device, can be said to be a systematic anoxic experimental tool integrating constant pressure, dynamic monitoring of respiratory frequency, amplitude, oxygen consumption and anoxic environmental pressure, overcomes the problem that the old device in the background technology can not recover data when reading oxygen consumption data by a visual method and missing an acquisition time point, lightens labor intensity, and has the advantages of low manufacturing cost, simple manufacture and suitability for teaching and scientific research.
Compared with the old device, the invention has the following advantages:
the invention uses the biological signal acquisition and processing system to record the dynamic oxygen consumption, can acquire the oxygen consumption rate in any time period in the oxygen deficiency process, overcomes the problem that the old device can not recover the data when the old device reads the oxygen consumption data by a visual method and misses the acquisition time point, and lightens the labor intensity; the method can change the conventional method of using the limited oxygen consumption in the case of oxygen deficiency, and change the method of using the time oxygen consumption rate or the minute oxygen consumption to obtain an experimental result, thereby avoiding the damage of the limited oxygen consumption to animals; particularly, the horizontal liquid column is adopted during the experiment, so that the experiment precision is not influenced.
The embodiments described in this specification are merely illustrative of implementations of the inventive concept and the scope of the present invention should not be considered limited to the specific forms set forth in the embodiments but includes equivalent technical means as would be recognized by those skilled in the art based on the inventive concept.
Claims (7)
1. A real-time respiratory oxygen consumption monitoring device with an upper drainage structure comprises a horizontally laid body, wherein a capacity groove, an oxygen consumption monitoring groove and an air inlet guide groove are engraved in the body, and an air inlet and an air outlet for communicating an anoxic bottle are arranged on the upper surface of the body; the air inlet is communicated with the capacity groove through an air inlet guide groove; one end of the oxygen consumption monitoring groove is communicated with the volumetric groove, and the other end of the oxygen consumption monitoring groove is communicated with the gas outlet; the air outlet is connected with the oxygen-deficient bottle through a high-sensitivity transducer for collecting respiratory motion data, and the high-sensitivity transducer is connected with a biological signal collecting and processing system;
a bulge is arranged at the joint of the capacity tank and the oxygen consumption monitoring tank, and the bulge shields part of the cross section of the inlet of the oxygen consumption monitoring tank;
scales are arranged on the upper surface of the body along the length direction of the oxygen consumption monitoring groove;
the method is characterized in that: the body is a colorless transparent acrylic plate, and the lower surface of the body is tightly attached to the flat shading plate;
a plurality of photoresistors are uniformly arranged on the bottom of the oxygen consumption monitoring groove along the length direction of the oxygen consumption monitoring groove; the photoresistors are sequentially connected in series to form a sampling resistor, the sampling resistor is connected to a resistor detection circuit, the output end of the resistor detection circuit is connected with a biological signal acquisition and processing system, and the biological signal acquisition and processing system outputs animal oxygen consumption data according to the data of the sampling resistor;
the oxygen consumption monitoring groove has a serpentine shape; the oxygen consumption monitoring groove comprises a plurality of sections of straight grooves which are parallel to each other and a curved groove which is connected with two adjacent straight grooves;
a plurality of drainage guide grooves and a backwater guide groove are engraved in the body, and outlets of the drainage guide grooves are communicated with the capacity groove and the air inlet guide groove through the backwater guide groove;
the oxygen consumption monitoring groove is communicated with the inlet of each drainage guide groove through a backflow-preventing guide device, each guide device comprises a guide block and a drainage pit covered on the guide block, and a gap communicated with the oxygen consumption monitoring groove and the drainage guide groove is formed between each drainage pit and the guide block;
the drainage pit is arranged on the body, and the flow guide blocks are respectively and fixedly arranged between each curved groove close to one side of the drainage guide groove and the drainage guide groove; defining one end of the flow guide block close to the curved groove as a left end and one end close to the drainage guide groove as a right end; the bottom surface of water conservancy diversion piece and the top surface looks parallel and level of oxygen consumption monitoring groove, the top surface of water conservancy diversion piece is the inclined plane that rises from the left end to the right end slope gradually, just the left end on inclined plane extends to curved groove department, the right-hand member on inclined plane extends to in the drainage guide slot.
2. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 1, wherein: the groove depths of the capacity groove, the oxygen consumption monitoring groove and the air inlet guide groove are 0.5 cm.
3. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 2, wherein: the air inlet and the air outlet are both positioned at the upper left side of the body, and the capacity groove is positioned at the lower right side of the body.
4. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 3, wherein: the oxygen-deficient bottle and the concrete communicating structure of the gas outlet are as follows: one side of the body is provided with a conversion interface communicated with the gas outlet, the other side of the body is provided with an oxygen deficiency bottle external interface communicated with the oxygen deficiency bottle, and the conversion interface is communicated with the oxygen deficiency bottle external interface through a high-sensitivity transducer.
5. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 4, wherein: the air outlet and the conversion interface are detachably connected through a connecting pipe.
6. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 5, wherein: the straight groove horizontally extends from left to right on the body, and the straight groove is vertically connected with the bent groove.
7. The device for monitoring the oxygen consumption of breath with the upper drainage structure in real time as claimed in claim 6, wherein: the flow guide block is a wedge-shaped block.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810149643.0A CN108514416B (en) | 2018-02-13 | 2018-02-13 | A real-time monitoring device for respiratory oxygen consumption with upper drainage structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810149643.0A CN108514416B (en) | 2018-02-13 | 2018-02-13 | A real-time monitoring device for respiratory oxygen consumption with upper drainage structure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108514416A CN108514416A (en) | 2018-09-11 |
| CN108514416B true CN108514416B (en) | 2020-04-21 |
Family
ID=63433216
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810149643.0A Expired - Fee Related CN108514416B (en) | 2018-02-13 | 2018-02-13 | A real-time monitoring device for respiratory oxygen consumption with upper drainage structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108514416B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110623670B (en) * | 2019-08-16 | 2020-07-10 | 浙江大学 | A split type real-time monitoring device for respiratory oxygen consumption |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340629A (en) * | 2013-05-29 | 2013-10-09 | 浙江大学 | Method for dynamically determining breathing oxygen consumption and hypoxia device for same |
| CN104267166A (en) * | 2014-10-13 | 2015-01-07 | 吉林市光大分析技术有限责任公司 | Rapid biological oxygen consumption detection device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8109884B2 (en) * | 2005-09-23 | 2012-02-07 | Kitchener Clark Wilson | Dynamic metabolism monitoring system |
-
2018
- 2018-02-13 CN CN201810149643.0A patent/CN108514416B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340629A (en) * | 2013-05-29 | 2013-10-09 | 浙江大学 | Method for dynamically determining breathing oxygen consumption and hypoxia device for same |
| CN104267166A (en) * | 2014-10-13 | 2015-01-07 | 吉林市光大分析技术有限责任公司 | Rapid biological oxygen consumption detection device |
Non-Patent Citations (1)
| Title |
|---|
| 小鼠整体氧耗的动态测定;蒋志文等;《中国药理学通报》;19961231;第12卷(第6期);567-568页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108514416A (en) | 2018-09-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108272455B (en) | Real-time oxygen consumption monitoring system for small animals with modularized water-seal anoxic bottles | |
| CN108742632B (en) | Real-time oxygen consumption monitoring system for small animals with hypoxic anoxic bottles | |
| CN103340629B (en) | Method for dynamically determining breathing oxygen consumption and hypoxia device for same | |
| CN106840312A (en) | A kind of crude oil storage tank water liquid level measurement mechanism for oil and method | |
| CN102854221A (en) | In-situ measurement device and method for sediment breathing and nitrogen endogenous release | |
| CN113607796B (en) | Microfluid flow/flow rate and component cooperative detection device and application thereof | |
| CN108514416B (en) | A real-time monitoring device for respiratory oxygen consumption with upper drainage structure | |
| CN207423789U (en) | A kind of liquid transparency detection device | |
| CN219104651U (en) | Suspended load sand content and grading field measurement device | |
| CN201141824Y (en) | A kind of turbidimeter for testing the turbidity of bacterial liquid | |
| CN108514417B (en) | Respiration oxygen consumption real-time monitoring device with coiling oxygen consumption monitoring groove | |
| CN108433726B (en) | Respiration oxygen consumption real-time monitoring device | |
| CN210953907U (en) | Sewage treatment plant toxicity of intaking on-line measuring device | |
| CN110623670B (en) | A split type real-time monitoring device for respiratory oxygen consumption | |
| CN119470211B (en) | Integrated device and method for comprehensively evaluating and measuring filtering performance of filtering material of filter tank | |
| CN105771668B (en) | A kind of hollow fiber film assembly integrity detection device and its detection method | |
| CN201697681U (en) | Light control metering device | |
| CN119533593A (en) | A flow meter detection device | |
| CN207366409U (en) | A kind of instrument for measuring permeability coefficient | |
| CN204374083U (en) | A kind of online turbidimeter with flow control | |
| CN206146926U (en) | A soil intelligent disintegration instrument | |
| CN209878801U (en) | Filter backwash tester and its filter backwash test system | |
| CN218089116U (en) | A device for simulating the cycle and removal mechanism of nitrogen and phosphorus pollutants under the change of water level in wetland lakes | |
| CN211291596U (en) | Automatic flow measurement device for open channel based on dilution method | |
| CN206040067U (en) | An Improved Experimental Device for Water Head Loss Along the Way |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200421 |