CN215328133U - Temperature-controllable incubator with vibration and vacuum bubble removing functions - Google Patents
Temperature-controllable incubator with vibration and vacuum bubble removing functions Download PDFInfo
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- CN215328133U CN215328133U CN202121751367.9U CN202121751367U CN215328133U CN 215328133 U CN215328133 U CN 215328133U CN 202121751367 U CN202121751367 U CN 202121751367U CN 215328133 U CN215328133 U CN 215328133U
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Abstract
The utility model discloses a temperature-controllable incubator with vibration and vacuum bubble removal functions, and particularly relates to the technical field of medical molecular biology experiments. According to the utility model, the time of vacuum and vibration can be accurately controlled by utilizing the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and the program, so that bubbles on the liquid surface of the sample in the temperature control culture cabin are extruded and broken, gas is sucked into the sealed vacuum buffer container and fine bubbles are completely removed, and the accuracy of an experimental result can be ensured.
Description
Technical Field
The utility model relates to the technical field of medical molecular biology experiments, in particular to a temperature-controllable incubator with vibration and vacuum bubble removal functions.
Background
Real-time fluorescent Quantitative PCR (Quantitative Real-time PCR) is a method for measuring the total amount of products after each Polymerase Chain Reaction (PCR) cycle by using fluorescent chemical substances in DNA amplification reaction. A method for quantitatively analyzing a specific DNA sequence in a sample to be detected by an internal reference method or an external reference method. In the process of configuring the reaction system, air bubbles need to be removed after all components are added. Enzyme linked immunosorbent assay (ELISA), which is a qualitative and quantitative detection method in which soluble antigen or antibody is bound to a solid phase carrier such as polystyrene and immunoreaction is carried out by utilizing specific binding of antigen and antibody. In the experiment process, bubbles are easy to generate sometimes in order to clean the liquid in the gun head, and usually the bubbles are gathered around the holes of the elisa plate, so that the liquid in the holes can not effectively contact with the hole walls, and the reaction in the holes is not uniform.
Currently, research or medical laboratories remove air bubbles by flicking the reaction vessel, centrifuging or hot air treatment. The removal effect of the flick reaction container on bubbles is limited, and fine bubbles cannot be removed; the centrifugation time is long, the liquid is easy to stratify, the hot air treatment changes the reaction conditions in the experimental design, the enzyme activity is influenced, meanwhile, the uneven heating increases the experimental variables, the experimental result is easy to deviate from the real result, and thus, the wrong conclusion is obtained. Therefore, the removal of air bubbles in qPCR and ELISA experimental samples is a problem to be solved urgently.
SUMMERY OF THE UTILITY MODEL
Therefore, the utility model provides the temperature-controllable incubator with the functions of vibration and vacuum bubble removal, the vacuum and vibration time can be accurately controlled by utilizing the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and the program, so that bubbles on the liquid surface of a sample in the temperature-controllable incubator are extruded and broken, gas is sucked into the sealed vacuum buffer container and the tiny bubbles are completely removed, the accuracy of an experimental result can be ensured, and the problems that the accuracy of the experimental result is easily influenced due to the fact that a flick reaction container cannot remove tiny bubbles, the centrifugation is easy to delaminate and the hot air treatment is easy to be heated unevenly in the prior art are solved.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions.
A temperature-controllable incubator with vibration and vacuum bubble removal functions comprises a temperature-controllable culture cabin, wherein a PTC heating module is fixedly arranged at the bottom end of the inner portion of the temperature-controllable culture cabin, a refrigerating sheet is arranged at the top of the PTC heating module, a PCR tube support is arranged at the top of the refrigerating sheet, the PCR tube support and the refrigerating sheet are both arranged inside the temperature-controllable culture cabin, a pneumatic vibrator is arranged at the top of the temperature-controllable culture cabin, a sealed vacuum buffer container is arranged at the top of the pneumatic vibrator, an electromagnetic valve group is arranged at the top of the sealed vacuum buffer container, the bottom of the electromagnetic valve group is fixedly connected with the top of the sealed vacuum buffer container, a first connector is arranged on one side of the temperature-controllable culture cabin and the front side of the electromagnetic valve group, two first connectors are connected through an air pipe, a second connector is arranged on the rear sides of the sealed vacuum buffer container and the electromagnetic valve group, and the two connectors are connected through an air pipe, sealed vacuum buffer container one side and electromagnetism valves front side all are equipped with and connect three, two connect through the trachea between three and connect, are located the three of joint of sealed vacuum buffer container one side is established at two front sides of joint, is located the joint tribit of electromagnetism valves front side is located a joint top, electromagnetism valves rear side is equipped with air compressor connecting tube and vacuum pump connecting tube, the vacuum pump connecting tube is established at air compressor connecting tube top, the pneumatic vibrator front side communicates through trachea and electromagnetism valves front side.
Furthermore, one end of the air compressor connecting pipe is communicated with the air compressor, and one end of the vacuum pump connecting pipe is communicated with the vacuum pump.
Further, the solenoid valve group comprises a first solenoid valve, a second solenoid valve and a third solenoid valve, wherein the third solenoid valve is fixedly arranged at the top of the sealed vacuum buffer container, the second solenoid valve is fixedly arranged at the top of the third solenoid valve, the first solenoid valve is fixedly arranged at the top of the second solenoid valve, one of the three connectors is arranged at the front side of the first solenoid valve, one of the three connectors is arranged at the front side of the second solenoid valve, the other connector is arranged at the rear side of the second solenoid valve, a vacuum pump connecting pipe is arranged at the rear side of the first solenoid valve, the vacuum pump connecting pipe is arranged at one side of the second connector, and an air compressor connecting pipe is arranged at the rear side of the second solenoid valve.
Further, cabin hatch door is cultivateed to control by temperature change cultivation cabin front side through hinge swing joint, cabin hatch door is cultivateed to control by temperature change inside fixed the inlaying of cabin hatch door has transparent observation window, transparent observation window is made by transparent material.
Furthermore, a sealed vacuum buffer container fixing support is arranged at the outer end of the pneumatic vibrator, the bottom of the sealed vacuum buffer container fixing support is fixedly connected with the top of the temperature control culture cabin, and the top of the sealed vacuum buffer container fixing support is fixedly connected with the bottom of the sealed vacuum buffer container.
Furthermore, the PTC heating module and the refrigerating sheet form a temperature control system.
The beneficial effects of the utility model are as follows.
1. The utility model can accurately control the vacuum and vibration time by utilizing the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve and the program, so that bubbles on the liquid surface of the sample in the temperature control culture cabin are extruded and broken, and the gas is sucked into the sealed vacuum buffer container and completely removed.
2. According to the utility model, the temperature in the temperature-controlled culture cabin is changed by controlling the PTC heating module and the refrigerating sheet to form a culture environment, the sample is cultured, and the temperature is observed through the transparent observation window until the experiment is finished, so that the temperature can be directly set after bubbles are removed, the sample is cultured and tested, the contact pollution of the sample and the outside is reduced, and the accuracy of the experiment result can be ensured.
3. The utility model can prepare qPCR reaction solution, compared with the traditional operation on ice, the utility model avoids the problem that the melted ice water pollutes the reaction solution; in addition, the utility model can perform ELISA incubation without preparing an additional incubator.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
Fig. 1 is a schematic view of the overall structure provided by the present invention.
Fig. 2 is a front view provided by the present invention.
Fig. 3 is a schematic structural view of a fixing bracket of a sealed vacuum buffer container provided by the utility model.
FIG. 4 is a schematic structural diagram of a temperature-controlled culture chamber and a temperature-controlled culture chamber door provided by the utility model.
Fig. 5 is an enlarged view of a in fig. 1 according to the present invention.
In the figure: the device comprises a temperature control culture cabin 1, a pneumatic vibrator 2, a sealed vacuum buffer container 3, an electromagnetic valve group 4, an air compressor connecting pipe 5, a vacuum pump connecting pipe 6, a PTC heating module 7, a refrigerating sheet 8, a PCR pipe support 9, a sealed vacuum buffer container fixing support 10, an electromagnetic valve I11, an electromagnetic valve II 12, an electromagnetic valve III 13, a temperature control culture cabin door 14, a transparent observation window 15, a connector I16, a connector II 17 and a connector III 18.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the utility model will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the utility model and that it is not intended to limit the utility model to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to the attached drawings 1-5 in the specification, the temperature-controllable incubator with vibration and vacuum bubble removal functions comprises a temperature-controllable culture cabin 1, a PTC heating module 7 is fixedly arranged at the bottom end inside the temperature-controllable culture cabin 1, a refrigerating sheet 8 is arranged at the top of the PTC heating module 7, a temperature control system is formed by the PTC heating module 7 and the refrigerating sheet 8 and used for controlling the temperature in the experimental process, a PCR tube support 9 is arranged at the top of the refrigerating sheet 8, the PCR tube support 9 and the refrigerating sheet 8 are both arranged inside the temperature-controllable culture cabin 1, a pneumatic vibrator 2 is arranged at the top of the temperature-controllable culture cabin 1, the pneumatic vibrator 2 is positioned between the temperature-controllable culture cabin 1 and a sealed vacuum buffer container 3 and can vibrate bubbles below the liquid level to the liquid level during vibration so as to be conveniently removed, the sealed vacuum buffer container 3 is arranged at the top of the pneumatic vibrator 2, pneumatic vibrator 2 outer end is equipped with sealed vacuum buffer container fixed bolster 10, 1 top fixed connection in cabin is cultivateed with the control by temperature change in sealed vacuum buffer container fixed bolster 10 bottom, sealed vacuum buffer container fixed bolster 10 top and 3 bottom fixed connection of sealed vacuum buffer container are convenient for support sealed vacuum buffer container 3 from the bottom through setting up sealed vacuum buffer container fixed bolster 10, but also can cultivate the cabin 1 with sealed vacuum buffer container 3 with pneumatic vibrator 2 restriction in the control by temperature change between. The top of the sealed vacuum buffer container 3 is provided with an electromagnetic valve group 4, the electromagnetic valve group 4 comprises a first electromagnetic valve 11, a second electromagnetic valve 12 and a third electromagnetic valve 13, the third electromagnetic valve 13 is fixedly arranged at the top of the sealed vacuum buffer container 3, the second electromagnetic valve 12 is fixedly arranged at the top of the third electromagnetic valve 13, the first electromagnetic valve 11 is fixedly arranged at the top of the second electromagnetic valve 12, the bottom of the electromagnetic valve group 4 is fixedly connected with the top of the sealed vacuum buffer container 3, a first connector 16 is arranged on one side of the temperature control culture cabin 1 and the front side of the electromagnetic valve group 4, the first connectors 16 are connected with each other through an air pipe, one connector 16 is arranged on the front side of the second electromagnetic valve 12, a second connector 17 is arranged on the rear sides of the sealed vacuum buffer container 3 and the electromagnetic valve group 4, the second connectors 17 are connected with each other through an air pipe, one connector 17 is arranged on the rear side of the first electromagnetic valve 11, sealed vacuum buffer container 3 one side and 4 front sides of electromagnetism valves all are equipped with and connect three 18, two connect through the trachea between three 18 and connect, are located the three 18 of joint of sealed vacuum buffer container 3 one side are established and are being connected two 17 front sides, are located the three 18 of joint of 4 front sides of electromagnetism valves are located a 16 tops of joint, 4 rear sides of electromagnetism valves are equipped with air compressor connecting tube 5 and vacuum pump connecting tube 6, vacuum pump connecting tube 6 is established at 5 tops of air compressor connecting tube, 5 one ends of air compressor connecting tube and air compressor intercommunication, 6 one ends of vacuum pump connecting tube and vacuum pump intercommunication, vibration and the vacuum function that can control whole equipment through intercommunication air compressor and vacuum pump. The front side of the pneumatic vibrator 2 is communicated with the front side of the electromagnetic valve group 4 through an air pipe, one of the joints III 18 is arranged on the front side of the first electromagnetic valve 11, the vacuum pump connecting pipe 6 is arranged on the rear side of the first electromagnetic valve 11, the vacuum pump connecting pipe 6 is arranged on one side of the joint II 17, and the air compressor connecting pipe 5 is arranged on the rear side of the second electromagnetic valve 12.
The implementation scenario is specifically as follows: firstly, the utility model is communicated with an air compressor and a vacuum pump through an air compressor connecting pipe 5 and a vacuum pump connecting pipe 6, then a power supply is switched on, a temperature control culture cabin door 14 is opened, a prepared PCR pipe bracket 9 with a sample to be cultured is sent into a temperature control culture cabin 1, the temperature control culture cabin door 14 is closed, the utility model is controlled through a program connected with a computer, a signal is given to a first electromagnetic valve 11, the interior of a sealed vacuum buffer container 3 starts to generate a vacuum environment, a signal is given to a second electromagnetic valve 12, a pneumatic vibrator 2 starts to work to generate vibration, bubbles below the liquid level are vibrated out to be above the liquid level, the internal sample is observed through a transparent observation window 15, the signal is given to the second electromagnetic valve 12 through the program after the bubbles float upwards, the pneumatic vibrator 2 stops vibrating, at the moment, the signal is given to the first electromagnetic valve 11, the sealed vacuum buffer container 3 stops continuously forming vacuum, the air pressure in the sealed vacuum buffer container 3 is smaller than the air pressure in the temperature control culture cabin 1, a signal is sent to the electromagnetic valve III 13, the pipeline between the temperature control culture cabin 1 and the sealed vacuum buffer container 3 is communicated, bubbles on the liquid level of a sample in the temperature control culture cabin 1 are extruded and broken due to pressure difference, gas is sucked into the sealed vacuum buffer container 3, after the bubbles are observed through the transparent observation window 15, when the bubbles are completely removed, the electromagnetic valve I11, the electromagnetic valve II 12 and the electromagnetic valve III 13 can be completely stopped, the temperature in the temperature control culture cabin 1 is changed by controlling the PTC heating module 7 and the refrigerating sheet 8 to form a culture environment, the sample is cultured, and the sample is observed through the transparent observation window 15 until the experiment is finished, the utility model has the advantages of simple and convenient operation, small volume and space saving, can simultaneously remove the bubbles from a plurality of sample liquid levels and can remove the tiny bubbles below the liquid levels, the vacuum and vibration time can be accurately controlled through the first electromagnetic valve 11, the second electromagnetic valve 12, the third electromagnetic valve 13 and a computer program; the temperature can be directly set after bubbles are removed, the sample is cultured and tested, the contact pollution of the sample and the outside is reduced, and therefore the accuracy of the test result can be guaranteed.
Although the utility model has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the utility model. Accordingly, such modifications and improvements are intended to be within the scope of the utility model as claimed.
Claims (6)
1. The utility model provides a but temperature control incubator with vibration and vacuum degassing bubble function, cultivates cabin (1) including the control by temperature change, its characterized in that: the temperature control culture cabin is characterized in that a PTC heating module (7) is fixedly arranged at the bottom end inside the temperature control culture cabin (1), a refrigerating sheet (8) is arranged at the top of the PTC heating module (7), a PCR tube support (9) is arranged at the top of the refrigerating sheet (8), the PCR tube support (9) and the refrigerating sheet (8) are both arranged inside the temperature control culture cabin (1), a pneumatic vibrator (2) is arranged at the top of the temperature control culture cabin (1), a sealed vacuum buffer container (3) is arranged at the top of the pneumatic vibrator (2), an electromagnetic valve group (4) is arranged at the top of the sealed vacuum buffer container (3), the bottom of the electromagnetic valve group (4) is fixedly connected with the top of the sealed vacuum buffer container (3), a first connector (16) is arranged on one side of the temperature control culture cabin (1) and the front side of the electromagnetic valve group (4), the first connectors (16) are connected through an air pipe, and a second connector (17) is arranged on the rear sides of the sealed vacuum buffer container (3) and the electromagnetic valve group (4), two connect through the trachea between two (17) and connect, sealed vacuum buffer container (3) one side and electromagnetism valves (4) front side all are equipped with and connect three (18), two connect through the trachea between three (18), are located the three (18) of joint of sealed vacuum buffer container (3) one side are established and are connected two (17) front side, are located the three (18) of joint of electromagnetism valves (4) front side are located and connect one (16) top, electromagnetism valves (4) rear side is equipped with air compressor connecting pipe (5) and vacuum pump connecting pipe (6), establish at air compressor connecting pipe (5) top vacuum pump connecting pipe (6), pneumatic vibrator (2) front side communicates through trachea and electromagnetism valves (4) front side.
2. The temperature-controllable incubator with vibration and vacuum bubble removal functions as claimed in claim 1, wherein: one end of the air compressor connecting pipe (5) is communicated with the air compressor, and one end of the vacuum pump connecting pipe (6) is communicated with the vacuum pump.
3. The temperature-controllable incubator with vibration and vacuum bubble removal functions as claimed in claim 1, wherein: solenoid valve group (4) include solenoid valve (11), two (12) of solenoid valve and three (13) of solenoid valve, three (13) of solenoid valve are fixed to be established at sealed vacuum buffer container (3) top, two (12) of solenoid valve are fixed to be established at three (13) tops of solenoid valve, two (11) of solenoid valve are fixed to be established at two (12) tops of solenoid valve, one of them three (18) of joint are established at one (11) front side of solenoid valve, one of them joint (16) are established at two (12) front sides of solenoid valve, one of them joint two (17) are established at solenoid valve one (11) rear side, establish at one (11) rear side of solenoid valve vacuum pump connecting pipe (6), establish in two (17) one side of joint vacuum pump connecting pipe (6), establish at two (12) rear sides of solenoid valve in air compressor connecting pipe (5).
4. The temperature-controllable incubator with vibration and vacuum bubble removal functions as claimed in claim 1, wherein: cabin hatch door (14) is cultivateed to control by temperature change cultivation cabin (1) front side through hinge swing joint, cabin hatch door (14) is cultivateed to control by temperature change inside fixed the inlaying of cabin hatch door (14) has transparent observation window (15), transparent observation window (15) are made by transparent material.
5. The temperature-controllable incubator with vibration and vacuum bubble removal functions as claimed in claim 1, wherein: the pneumatic vibration device is characterized in that a sealed vacuum buffer container fixing support (10) is arranged at the outer end of the pneumatic vibration device (2), the bottom of the sealed vacuum buffer container fixing support (10) is fixedly connected with the top of the temperature control culture cabin (1), and the top of the sealed vacuum buffer container fixing support (10) is fixedly connected with the bottom of the sealed vacuum buffer container (3).
6. The temperature-controllable incubator with vibration and vacuum bubble removal functions as claimed in claim 1, wherein: and the PTC heating module (7) and the refrigerating sheet (8) form a temperature control system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121751367.9U CN215328133U (en) | 2021-07-30 | 2021-07-30 | Temperature-controllable incubator with vibration and vacuum bubble removing functions |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121751367.9U CN215328133U (en) | 2021-07-30 | 2021-07-30 | Temperature-controllable incubator with vibration and vacuum bubble removing functions |
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| CN215328133U true CN215328133U (en) | 2021-12-28 |
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| CN202121751367.9U Active CN215328133U (en) | 2021-07-30 | 2021-07-30 | Temperature-controllable incubator with vibration and vacuum bubble removing functions |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114535210A (en) * | 2022-02-25 | 2022-05-27 | 天津市成吉斯宾科技发展有限公司 | Ultrasonic vacuum surface treatment cleaning method |
-
2021
- 2021-07-30 CN CN202121751367.9U patent/CN215328133U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114535210A (en) * | 2022-02-25 | 2022-05-27 | 天津市成吉斯宾科技发展有限公司 | Ultrasonic vacuum surface treatment cleaning method |
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