CN209113928U - Multilayer culture and antipollution attached cell culture apparatus - Google Patents
Multilayer culture and antipollution attached cell culture apparatus Download PDFInfo
- Publication number
- CN209113928U CN209113928U CN201821567811.XU CN201821567811U CN209113928U CN 209113928 U CN209113928 U CN 209113928U CN 201821567811 U CN201821567811 U CN 201821567811U CN 209113928 U CN209113928 U CN 209113928U
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- bottle
- culture
- tube
- cell
- antipollution
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Abstract
The utility model relates to a kind of multilayer culture and antipollution attached cell culture apparatus, including heating oscillator, culture bottle, returnable bottle and reagent bottle, it heats oscillator top and one spring pedestal is installed, spring pedestal upper level places culture bottle, culture bottle bottle sealing installs bottle cap, bottle cap is towards side, bottle sealing installs a liquid-feeding tube and a recovery tube, recycling tube end is located at culture bottle bottom, liquid-feeding tube connection connection Five-way tube, Five-way tube four additional connecting tube is separately connected a reagent bottle, control valve is installed in connecting tube, recovery tube connection connection tee tube, other two connecting tube of tee tube is separately connected a returnable bottle, control valve is installed in connecting tube.The culture bottle of cell culture apparatus provided by the present application is of five storeys cell culture layer, almost 5 times of Nostoc commune Vanch bottle culture cell number, facilitates the culture of high-volume cell, various reagents, consumptive material have been saved in cell cultivation process, save the space of CO2 incubator.
Description
Technical field
The utility model belongs to experimental provision field, especially a kind of multilayer culture and antipollution attached cell culture dress
It sets.
Background technique
In biotechnology, cell culture is an essential experimentation.It is thin that general cell is divided into suspension
Born of the same parents and two kinds of attached cell, wherein the growth of attached cell must have the support surface that can be attached, and cell is relied on from status
The attaching factor provided in secrete or culture medium could growth and breeding on a surface.Our existing laboratory cultures are adherent thin
Born of the same parents, which are mainly attached on the bottom wall of culture bottle, to be grown, and existing attached cell culture apparatus has the disadvantage that
1. the culture bottle floor space that existing attached cell culture is used is limited, when high-volume being needed to cultivate attached cell,
Expend a large amount of reagent, consumptive material and the big quantity space for occupying CO2 incubator；
2. the upgrowth situation that existing multilayer attached cell culture bottle cannot observe every one layer of cells；
3. including multiple processes such as passage, preparation in attached cell incubation, need using different agents on cellular
It is handled, needs repeatedly to open culture bottle cap during this, is added or is sucked out reagent with pipette disengaging culture bottle,
It is highly contaminating；
4., since the function and effect and temperature of pancreatin have very big relationship, it is latter that pancreatin is added during cell dissociation
As culture bottle can be all put into in incubator the action time for accelerating pancreatin, on the one hand make experimental implementation cumbersome in this way, it is another
Aspect increases the possibility of incubator pollution.
Utility model content
The purpose of the utility model is to overcome in place of the deficiencies in the prior art, provide a kind of good fixing effect, can heat,
The multilayer culture that can be shaken and antipollution attached cell culture apparatus.
The utility model solves its technical problem and adopts the following technical solutions to achieve:
A kind of multilayer culture and antipollution attached cell culture apparatus, it is characterised in that: including heating oscillator, culture
A spring pedestal is installed on bottle, returnable bottle and reagent bottle, heating oscillator top, and spring pedestal upper level places culture bottle,
Culture bottle bottle sealing installs bottle cap, and bottle cap installs a liquid-feeding tube and a recovery tube towards side, bottle sealing, recycles tube end
Positioned at culture bottle bottom, liquid-feeding tube connection connection Five-way tube, Five-way tube four additional connecting tube is separately connected a reagent bottle,
Control valve, recovery tube connection connection tee tube are installed, other two connecting tube of tee tube is separately connected a recycling in connecting tube
Bottle, installs control valve in connecting tube, and piston is installed in returnable bottle lower part；
The structure of the culture bottle is as follows: in culture bottle on the corresponding side wall of bottleneck horizontal interval system there are five support plate,
Five confluent monolayer cells culture layers are formed, there are baffle in each support plate two sides.
Moreover, support plate bottom surface is obliquely installed, the support plate of bottle mouth position is thin side.
Moreover, being formed with an interlayer, the embedded dress enlarging lens of interlayer in the side of culture bottle.
Moreover, placing PBS liquid, trypsin solution, cell culture medium and serum mixed liquor, cell respectively in four reagent bottles
Suspension.
The advantages and positive effects of the utility model are as follows:
1, the culture bottle of cell culture apparatus provided by the present application is of five storeys cell culture layer, and almost Nostoc commune Vanch bottle is trained
5 times for supporting cell number, facilitate the culture of high-volume cell, various reagents, consumptive material have been saved in cell cultivation process, have been saved
The space of CO2 incubator.
2, design has simple foldable microscope in the culture bottle of cell culture apparatus provided by the present application, helps to observe
The growth conditions of every confluent monolayer cells.
3, cell culture apparatus provided by the present application connects culture bottle and various examinations using Five-way tube and tee tube respectively
Agent bottle, returnable bottle avoid frequently switching on culture bottle cap due to using pipette, greatly reduce thin during experimental implementation
The contaminated possibility of born of the same parents.
4, the heating oscillator heating function of cell culture apparatus provided by the present application uses so that in cell dissociation mistake
Journey does not need culture bottle being put into CO2In incubator, to keep experimental implementation more convenient, while being greatly reduced due to frequency
It is numerous switch and make CO2The contaminated possibility of incubator.
5, heating oscillator the using for function of concussion of cell culture apparatus provided by the present application makes cell dissociation more
Add thoroughly；When injecting cell suspension cell can again be distributed in culture bottle more uniform.
Detailed description of the invention
Fig. 1 is the utility model attached cell culture apparatus structural schematic diagram.
Fig. 2 is the top view of culture bottle.
Specific embodiment
The utility model is described in further detail with reference to the accompanying drawing and by specific embodiment, and following embodiment is only retouched
The property stated, it is not restrictive, protection scope of this utility model cannot be limited by this.
A kind of multilayer culture and antipollution attached cell culture apparatus, including heating oscillator 14, culture bottle 1, recycling
A spring pedestal 15 is installed on bottle 11 and reagent bottle 7, heating oscillator top, and spring pedestal upper level places culture bottle, training
It supports bottle bottle sealing and bottle cap 5 is installed, bottle cap installs a liquid-feeding tube 6 and a recovery tube 4 towards side, bottle sealing, recycles pipe end
Portion is located at culture bottle bottom, wherein liquid-feeding tube connection connection Five-way tube 9, Five-way tube four additional connecting tube are separately connected one
Reagent bottle installs control valve 8, recovery tube connection connection tee tube 13, other two connecting tube of tee tube difference in connecting tube
A returnable bottle is connected, control valve 12 is installed in connecting tube, returnable bottle lower part installation piston 10 will be trained for manufacturing negative pressure
It supports in the draws fluid returnable bottle in bottle.
The structure of the culture bottle is as follows: culture bottle shape is similar with Nostoc commune Vanch bottle, need to will cultivate using when the present apparatus
Bottle cap is backed out, and device cap for special purpose is changed, in culture bottle on the corresponding side wall of bottleneck horizontal interval system there are five support plate 2,
Form five confluent monolayer cells culture layers, there are a baffle in each support plate two sides, and the height of each baffle is 1mm, between every layer and every layer
Spacing be 2mm, support plate bottom surface is obliquely installed, and the support plate of bottle mouth position is thin side, the side of culture bottle be formed with one every
Layer 16, the embedded dress enlarging lens 3 of interlayer, for watching the growing state of every confluent monolayer cells.
PBS liquid, trypsin solution, cell culture medium and serum mixed liquor, cell suspension are placed respectively in four reagent bottles.
Reagent area includes four sets of identical devices, and there are 4 fixed fishing without hooks in the top, hang with a reagent bottle below each fishing without hook
(PBS), (pancreatin), (cell culture medium and serum mixed liquor), (cell suspension), are connected with a Five-way tube below reagent bottle, and five
Four arrival ends of siphunculus are connected with each reagent bottle respectively, and there are four valve knob, outlet and the culture bottleneck phases of Five-way tube
Even；
Recovery area includes cell suspension returnable bottle, devil liquor recovery bottle and a tee tube, and the entrance of tee tube is close to training
Bottom of bottle portion is supported, two outlets are connected with two returnable bottles respectively, and there are two valve knobs；
Heat oscillator by pallet (for placing culture bottle), spring bracing, host, heater switch, concussion switch and
Attaching plug composition；
Cell suspension returnable bottle is collected be centrifuged, be resuspended, count, dilute etc. after cell suspension and operated after, be finally put into
It is dispensed into each culture bottle in reagent bottle (cell suspension).
The device of the utility model also has the advantage that
The cell culture layer 1. the culture bottle in the utility model is of five storeys, the culture of more convenient high-volume cell.
2. there is baffle on the both sides of 5 confluent monolayer cells culture layers in the culture bottle in the utility model, convenient for thin in cell suspension
Born of the same parents' being uniformly distributed in each layer, will not due to culture bottle shaking and so that cell is all stacked into the bottom.
3. design has simple foldable microscope in the culture bottle in the utility model, can move up and down to observe every layer
The growth conditions of cell.
4. the oblique frame of support between two layers of cell culture layer respectively by the bottom of upper one layer of cells culture layer right-hand apron with
The top of baffle is connected on the left of lower one layer of cells culture layer, by dextrad left bank, is conducive to cell suspension in this way from culture bottle
Mouth is evenly distributed in every one layer of cells culture layer after entering；If supporting oblique frame reverse design, cell suspension will be interfered to enter
Every confluent monolayer cells culture layer.
5. connecting culture bottle and various reagents bottle, returnable bottle using Five-way tube and tee tube respectively, avoid due to using
Pipette and frequently switch on culture bottle cap, greatly reduce the possibility of contamination of cells during experimental implementation.
6. heating oscillator heating function uses so that not needing culture bottle being put into CO in cell dissociation process2Culture
In case, to keep experimental implementation more convenient, while being greatly reduced due to frequently switching on and having made CO2Incubator is contaminated
It may.
7. using for heating oscillator concussion function makes the more thorough of cell dissociation；Make cell when injecting cell suspension
It is distributed in culture bottle more uniform.
Although disclosing the embodiments of the present invention and attached drawing for the purpose of illustration, those skilled in the art can be with
Understand: departing from the utility model and spirit and scope of the appended claims, various substitutions, changes and modifications are all can
Can, therefore, the scope of the utility model is not limited to the embodiment and attached drawing disclosure of that.
Claims (4)
1. a kind of multilayer culture and antipollution attached cell culture apparatus, it is characterised in that: including heating oscillator, culture
A spring pedestal is installed on bottle, returnable bottle and reagent bottle, heating oscillator top, and spring pedestal upper level places culture bottle,
Culture bottle bottle sealing installs bottle cap, and bottle cap installs a liquid-feeding tube and a recovery tube towards side, bottle sealing, recycles tube end
Positioned at culture bottle bottom, liquid-feeding tube connection connection Five-way tube, Five-way tube four additional connecting tube is separately connected a reagent bottle,
Control valve, recovery tube connection connection tee tube are installed, other two connecting tube of tee tube is separately connected a recycling in connecting tube
Bottle, installs control valve in connecting tube, and piston is installed in returnable bottle lower part；
The structure of the culture bottle is as follows: there are five support plates for horizontal interval system on the corresponding side wall of bottleneck in culture bottle, are formed
There are baffle in five confluent monolayer cells culture layers, each support plate two sides.
2. multilayer culture according to claim 1 and antipollution attached cell culture apparatus, it is characterised in that: support plate
Bottom surface is obliquely installed, and the support plate of bottle mouth position is thin side.
3. multilayer culture according to claim 1 and antipollution attached cell culture apparatus, it is characterised in that: cultivating
The side of bottle is formed with an interlayer, the embedded dress enlarging lens of interlayer.
4. multilayer culture according to claim 1 and antipollution attached cell culture apparatus, it is characterised in that: described four
PBS liquid, trypsin solution, cell culture medium and serum mixed liquor, cell suspension are placed in a reagent bottle respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821567811.XU CN209113928U (en) | 2018-09-26 | 2018-09-26 | Multilayer culture and antipollution attached cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821567811.XU CN209113928U (en) | 2018-09-26 | 2018-09-26 | Multilayer culture and antipollution attached cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209113928U true CN209113928U (en) | 2019-07-16 |
Family
ID=67199414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821567811.XU Expired - Fee Related CN209113928U (en) | 2018-09-26 | 2018-09-26 | Multilayer culture and antipollution attached cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209113928U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913603A (en) * | 2018-09-26 | 2018-11-30 | 天津市康婷生物工程有限公司 | A kind of recyclable multilayer culture and antipollution attached cell culture apparatus |
-
2018
- 2018-09-26 CN CN201821567811.XU patent/CN209113928U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108913603A (en) * | 2018-09-26 | 2018-11-30 | 天津市康婷生物工程有限公司 | A kind of recyclable multilayer culture and antipollution attached cell culture apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190716 |