CN203229543U - Semi-continuous high-density cell culture apparatus - Google Patents
Semi-continuous high-density cell culture apparatus Download PDFInfo
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- CN203229543U CN203229543U CN 201320269437 CN201320269437U CN203229543U CN 203229543 U CN203229543 U CN 203229543U CN 201320269437 CN201320269437 CN 201320269437 CN 201320269437 U CN201320269437 U CN 201320269437U CN 203229543 U CN203229543 U CN 203229543U
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- culturing bottle
- culture apparatus
- density cells
- cells culture
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Abstract
The utility model discloses a semi-continuous high-density cell culture apparatus. The semi-continuous high-density cell culture apparatus comprises a culture bottle, a stirring apparatus in the culture bottle, and a magnetic stirring apparatus, wherein the top of the culture bottle is provided with a first opening, a second opening and a third opening, the side wall of the culture bottle is provided with a fourth opening, and the culture bottle is arranged above the magnetic stirring apparatus. The semi-continuous high-density cell culture apparatus provided by the utility model has the advantages that the processing technology is simplified, fabrication materials are saved, and the fabrication cost is greatly reduced.
Description
Technical field
The utility model is applied to biological technical field, is specifically related to a kind of semicontinuous high-density cells culture apparatus.
Background technology
Be commonly the suspension culture form about the semi continuous cell cultures in the prior art.In cell cultivation process, at regular intervals, therefrom take out the part culture, pour into fresh culture again and continue to cultivate./ 2nd to 3/4ths of an each displacement volume of culture is with a kind of cell cultures pattern of the exponential growth state of keeping cell.This cell cultures pattern has repeatedly cultivates, the culture density height, and avoided inoculating repeatedly, cleaned, the advantage of complicated operations such as sterilization.
The semicontinuous Tissue Culture Flask that uses at present is the cylindrical culturing bottle of glass material, and the bottle wall is smooth, and lines are soft.Use such culturing bottle, can in culturing bottle, utilize the principle of magnetic agitation and water screw, by the even gentle cell culture medium that stirs, eukaryotic cell is carried out the high-density vitro culture.But require complicatedly because this culturing bottle is made complete processing, it is very difficult that expense costliness, common laboratory dispose use on a large scale.
The utility model content
The utility model has overcome culture apparatus complete processing complexity of the prior art, defective such as expensive, and a kind of semicontinuous high-density cells culture apparatus is provided.The utility model is simple in structure, cost is lower, can reach good semicontinuous high-density cells culture effect simultaneously.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, comprises culturing bottle, is arranged at the whipping appts in the described culturing bottle, and magnetic stirring apparatus; The top of described culturing bottle is provided with first opening, second opening and the 3rd opening, and the sidewall of described culturing bottle is provided with the 4th opening, and described culturing bottle is arranged on the top of described magnetic stirring apparatus.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and described culturing bottle is right cylinder.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and described first opening, described second opening, described the 3rd opening and described the 4th opening arrange sealing thread.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and described first opening is arranged on the central authorities at described culturing bottle top, and described second opening and described the 3rd opening are arranged at the both sides at described culturing bottle top respectively.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and the angle between described first opening and described second opening or described the 3rd opening is 45 °～60 °.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, further comprise, seal the sealing cover of described first opening, described second opening, described the 3rd opening and described the 4th opening by described sealing thread, at described second opening and described the 3rd opening breather line is set respectively, described breather line is provided with filter membrane device, and the aperture of described filter membrane device is 0.22～0.45 micron.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and described the 4th opening is arranged on bottom sixth to a four/place of the described culturing bottle of distance.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and described the 4th opening is arranged on the sidewall of described culturing bottle obliquely, and the sidewall of described the 4th opening and described culturing bottle is 30-45 °.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and liquid fluid infusion pipeline is got in described the 4th opening setting, and described getting on the liquid fluid infusion pipeline arranges filter membrane device.
The utility model provides a kind of semicontinuous high-density cells culture apparatus, and described whipping appts is magnetic stir bar or magnetic agitation oar.Magnetic stir bar places the bottom of culturing bottle, and rotation is stirred under the effect of magnetic stirring apparatus; For adapting to the requirement of different cell cultures, can adopt the magnetic agitation oar that inserts from the culturing bottle open top to replace magnetic stir bar.
Compare with common suspension culture device, the semicontinuous high-density cells culture apparatus of the utility model can make cell fully obtain oxygen, breeds well and grows, and at the uniform velocity stirs to make cell culture density improve; Also can take out culture in batches, add fresh culture, avoid inoculating repeatedly, clean, the advantage of complicated operations such as sterilization.Can be used for biological products or the preparations of cellular product in a large number such as the various recombinant proteins of external preparation.The utility model complete processing is simple, and the manufacturing price cost greatly reduces, only be in the market common suspension culture device 10%.
Description of drawings
Fig. 1 is the structural representation of semicontinuous high-density cells culture apparatus of the present utility model.
Fig. 2 is the structural representation of culturing bottle in the utility model.
Fig. 3 is the structural representation that is provided with the semicontinuous high-density cells culture apparatus of magnetic agitation oar.
Embodiment
In conjunction with following specific embodiments and the drawings, the utility model is described in further detail.Implement process of the present utility model, condition, experimental technique etc., except the following content of mentioning specially, be universal knowledege and the common practise of this area, the utility model is not particularly limited content.
As shown in Figure 1, semicontinuous high-density cells culture apparatus of the present utility model comprises culturing bottle 1, is arranged at whipping appts 2 and magnetic stirring apparatus 3 in the culturing bottle 1.Wherein, the top of culturing bottle 1 is provided with first opening 11, second opening 12 and the 3rd opening 13.The 4th opening 14 is set on the sidewall of culturing bottle 1.Culturing bottle 1 places on the magnetic stirring apparatus 3.Under the magneticaction of magnetic stirring apparatus 3, the whipping appts 2 in the culturing bottle 1 is rotated to stir realizes cell suspension culture.Magnetic stirring apparatus 3 is low speed regulation and control magnetic stirring apparatus.
As shown in Figure 2, culturing bottle 1 is the cylinder bodily form, and culturing bottle 1 can be that volume is 100ml, and the simple glass Tissue Culture Flask of 250ml or 500ml perhaps arranges voluntarily according to practical situation.In the present embodiment, culturing bottle 1 is the 250ml Tissue Culture Flask, and its rounded bottom diameter is 8cm, highly is 18 centimetres.First opening 11 of culturing bottle 1, second opening 12, the 3rd opening 13 and the 4th opening 14 places are provided with sealing thread.Culturing bottle 1 center of top is vertically installed with first opening 11.Second opening 12 and the 3rd opening 13 are separately positioned on the both sides at culturing bottle 1 top, and the angle between first opening 11 and second opening 12 or the 3rd opening 13 is 45 °～60 °.In the present embodiment, the angle of first opening 11 and second opening 12 is 45 °, and the angle of first opening 11 and the 3rd opening 13 is 45 °, and the angle between second opening 12 and the 3rd opening 13 is 90 °.All be arranged with sealing cover on second opening 12 and the 3rd opening 13, the sealing thread locking on sealing cover and second opening 12 and the 3rd opening 13, sealing covers and is embedded with breather line, is used for keeping when the semicontinuous cultivation of cell oxygen to feed.Also be provided with filter membrane device in the breather line, the aperture of filter membrane device is 0.22～0.45 micron.Be the filter membrane sheet of 0.22 micron pore size in the present embodiment, prevent that the impurity in the breather line from entering culturing bottle 1.The 4th opening 14 is 30～45 ° with the angle of culturing bottle 1 sidewall, and the 4th opening 14 is 45 and is arranged on culturing bottle 1 sidewall obliquely apart between bottom sixth to four/one in the present embodiment.In the present embodiment, the 4th opening 14 is arranged on apart from sixth place, culturing bottle 1 bottom, i.e. 2.6 centimeters.The 4th opening 14 keeps sealing by the sealing thread on it and sealing cover locking.Sealing covers and is provided with a Glass tubing as getting liquid fluid infusion pipeline.An end of getting liquid fluid infusion pipeline gos deep in the cell culture in the culturing bottle 1, and the other end has filter membrane device and connects and link to each other with a rubber hose, when being used for cell cultures to culturing bottle 1 taking-up culture and supply fresh culture.In the present embodiment, the filter membrane aperture is the 0.22-0.45 micron.The utility model does not limit the mode that breather line is set and gets liquid fluid infusion pipeline, breather line can also be set except sealing cover by other means and get liquid fluid infusion pipeline.
Be provided with whipping appts 2 in the culturing bottle 1, whipping appts 2 is magnetic stir bar.Culturing bottle 1 below is provided with magnetic stirring apparatus 3, and magnetic stirring apparatus 3 uses the magnetic force principle, and the rotation of the magnetic stir bar in the control culturing bottle 1 is stirred the cell in the substratum in the culturing bottle 1, thereby carried out cell suspension culture.For being adapted to cultivate the requirement of different cells, in case of necessity, the utility model can use the magnetic force water screw to substitute magnetic stir bar and stir.As shown in Figure 3, can insert the magnetic force water screw in first opening 11 of culturing bottle 1, the magnetic force water screw is rotated under the effect of magnetic stirring apparatus, thereby satisfies the needs of specific cell cultures.The semicontinuous high-density cells culture apparatus of the utility model can be used for the semicontinuous high-density suspension culture of various cells, simultaneously owing to used stirrer to substitute water screw of the prior art, simplify complete processing, saved the making material, thus the cost of manufacture that reduces greatly.
Protection content of the present utility model is not limited to above embodiment.Under the spirit and scope that do not deviate from the utility model design, variation and advantage that those skilled in the art can expect all are included in the utility model, and are protection domain with the appending claims.
Claims (10)
1. a semicontinuous high-density cells culture apparatus is characterized in that, comprises culturing bottle (1), is arranged at the whipping appts (2) in the described culturing bottle (1), and magnetic stirring apparatus (3); The top of described culturing bottle (1) is provided with first opening (11), second opening (12) and the 3rd opening (13), and the sidewall of described culturing bottle (1) is provided with the 4th opening (14), and described culturing bottle (1) is arranged on the top of described magnetic stirring apparatus (3).
2. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, described culturing bottle (1) is right cylinder.
3. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, described first opening (11), described second opening (12), described the 3rd opening (13) and described the 4th opening (14) arrange sealing thread.
4. semicontinuous high-density cells culture apparatus as claimed in claim 1, it is characterized in that, described first opening (11) is arranged on the central authorities at described culturing bottle (1) top, and described second opening (12) and described the 3rd opening (13) are arranged at the both sides at described culturing bottle (1) top respectively.
5. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, the angle between described first opening (11) and described second opening (12) or described the 3rd opening (13) is 45 °～60 °.
6. semicontinuous high-density cells culture apparatus as claimed in claim 1, it is characterized in that, further comprise, seal the sealing cover of described first opening (11), described second opening (12), described the 3rd opening (13) and described the 4th opening (14) by described sealing thread, at described second opening (12) and described the 3rd opening (13) breather line is set respectively, described breather line is provided with filter membrane device, and the aperture of described filter membrane device is 0.22～0.45 micron.
7. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, described the 4th opening (14) is arranged on bottom sixth to a four/place of the described culturing bottle of distance (1).
8. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, described the 4th opening (14) is arranged on the sidewall of described culturing bottle (1) obliquely, and described the 4th opening (14) is 30～45 ° with the sidewall of described culturing bottle (1).
9. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, described the 4th opening (14) setting is got liquid fluid infusion pipeline, and described getting on the liquid fluid infusion pipeline arranges filter membrane device.
10. semicontinuous high-density cells culture apparatus as claimed in claim 1 is characterized in that, described whipping appts (2) is magnetic stir bar or magnetic agitation oar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320269437 CN203229543U (en) | 2013-05-16 | 2013-05-16 | Semi-continuous high-density cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320269437 CN203229543U (en) | 2013-05-16 | 2013-05-16 | Semi-continuous high-density cell culture apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN203229543U true CN203229543U (en) | 2013-10-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320269437 Expired - Lifetime CN203229543U (en) | 2013-05-16 | 2013-05-16 | Semi-continuous high-density cell culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3034616B1 (en) * | 2014-12-17 | 2018-07-18 | Mohammad Salehi | Device for producing and/or cleaning nanoparticle coupled biomaterials and method using the same |
| CN110872562A (en) * | 2019-11-28 | 2020-03-10 | 武汉大学 | Extracellular vesicle batch production device |
-
2013
- 2013-05-16 CN CN 201320269437 patent/CN203229543U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3034616B1 (en) * | 2014-12-17 | 2018-07-18 | Mohammad Salehi | Device for producing and/or cleaning nanoparticle coupled biomaterials and method using the same |
| CN110872562A (en) * | 2019-11-28 | 2020-03-10 | 武汉大学 | Extracellular vesicle batch production device |
| CN110872562B (en) * | 2019-11-28 | 2023-03-28 | 武汉大学 | Extracellular vesicle batch production device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131009 |