CN101974424B - Swing-driven circular cell culture device - Google Patents
Swing-driven circular cell culture device Download PDFInfo
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- CN101974424B CN101974424B CN201010532912.5A CN201010532912A CN101974424B CN 101974424 B CN101974424 B CN 101974424B CN 201010532912 A CN201010532912 A CN 201010532912A CN 101974424 B CN101974424 B CN 101974424B
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- liquid storage
- storage chamber
- circulating line
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/24—Draft tube
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Abstract
The invention relates to a swing-driven circular cell culture device. The culture device comprises a hard culture container the lower part of which is provided with a solution chamber; the bottom surface of the solution chamber is a smooth concave surface; a slope surface is arranged on the side wall surface of the solution chamber; the slope surface and the concave surface are in smooth transition; a circular pipe is arranged on the side wall of the solution chamber along the slope surface in a matched manner; the circular pipe is provided with an upper opening and a lower opening; and a one-way discharge valve structure is arranged at the lower opening. When the circular cell culture device is driven to swing left and right, the upper culture solution constantly enters from the upper openings of the two circular pipes and rushes out from the lower openings, while the original lower culture solution is turned upwards in the process to realize up-down circulation of the culture solution; the circulation is simple and effective, and the mixing and ventilation effect is good; and moreover, the circulation frequency can be minimized and the shearing force of the circulation is little.
Description
Technical field
The present invention relates to a kind of when applying to wave driving for it, the nutrient solution in it can carry out the cell culture apparatus circulated.
Background technology
The cultivation of organism (comprises zooblast, vegetable cell, the cultivation of insect cell and microorganism) and utilize the organism of above-mentioned cultivation to carry out biological technology products (as protein, polypeptide class, nucleic acid, antibody class, virus vector and virus vaccines class) production, need to provide suitable temperature, necessary gas (as oxygen and carbon dioxide) and suitable mixing, therefore the various products adapted with it market have been there are, as thermostat container, CO2gas incubator, mixing shaking table, fermentor tank, culturing bottle and various bioreactors etc.When carrying out cell cultures, particularly animal cell culture and when utilizing zooblast to carry out the production of above-mentioned biological technology products, accurate temperature is often needed to control, good gas and nutrition exchange and the very little mixing of shearing force, therefore market there is various bio-reactor, as roller bottle bio-reactor, magnetic agitation bio-reactor, the basket bio-reactor of NBS and WAVE wave bio-reactor etc.
Containers for culturing organisms in the market for animal cell culture mainly contains rolling bottle, magnetic agitation culturing bottle, the basket resting cell culture bioreactors of NBS and WAVE shake pocket type bio-reactor, wherein rolling bottle and magnetic agitation culturing bottle are only for small-scale production with amplify step by step, because its non-once uses easy crossed contamination.In addition, magnetic agitation is cultivated and be there is the larger shortcoming of shearing force.NBS basket resting cell culture bioreactors is expensive, complicated operation, and be unsuitable for laboratory and cultivate on a small scale, its non-once culture tank exists cross-contamination issue.It is cell cultures cultivated in the soft bag of disposable plastic that WAVE shakes pocket type bio-reactor, producing wave, making cell suspension by shaking bag, nutrient solution mixes, gaseous interchange, its shearing force is relatively little, but there is following shortcoming: first, WAVE is mainly applicable to large-scale cell cultures, tool WAVE patent shows volume of culture preferably at more than 5L, when small volume is cultivated, can not produce enough waves and make cell suspension, cell deposition at the bottom of bag, phase mutual friction in shake process and damaging; Secondly, it is plastic soft bag that WAVE shakes bag, adds connected a series of pipelines, operates comparatively hardware culture vessel complicated; Again, the WAVE culture bag rocker matched of different model, pouch portion can use large rocker, and sack can not use little rocker, from cultivating the equipment needing to buy a series of costliness through being amplified to large-scale industrial production step by step on a small scale; Finally, although WAVE is little compared with stirring type bioreactor shearing force, necessary cell suspension be produced, fully mixing and gaseous interchange, also want certain sway velocity and frequency, therefore also can produce shearing force to a certain degree.
In order to overcome the shortcoming of above-mentioned bio-reactor, the particularly deficiency of WAVE swing reactor, must research and develop novel cell culture apparatus.
Summary of the invention
The object of the present invention is to provide the cyclical cell culture apparatus waving driving that a kind of shearing force is little, mixing ventilation effect is good.
For achieving the above object, the present invention adopts following technical scheme: a kind of cyclical cell culture apparatus waving driving, this culture apparatus comprises hard culture vessel, the bottom of this incubator is liquid storage chamber, and the bottom surface of liquid storage chamber is smooth concave surface, and the side wall surface of liquid storage chamber is slope, seamlessly transit between slope and concave surface, the sidewall of liquid storage chamber coincide along slope and is provided with circulating line, circulating line has upper shed and lower opening, and lower opening is provided with unidirectional venting valve structure.
The sidewall bag that described circulating line is arranged on film on the side wall surface of liquid storage chamber and liquid storage chamber by both side edges is established and is formed.
Described circulating line is formed by the identical body be installed on the sidewall of liquid storage chamber.
Described unidirectional venting valve structure is plural bag-shaped flexible valve, and the sack of bag-shaped flexible valve is towards the port of lower opening, and the outer side correspondence of bag-shaped flexible valve is arranged on the inwall of circulating line.
Described circulating line is soft pipeline, and bag-shaped flexible valve and circulating line are wholely set.
Described unidirectional venting valve structure is the paddle type limit diaphragm coincide with the pipe port shape of body, and limit diaphragm folding is sticked on the lower opening of body, and the outer rim of limit diaphragm is less than the periphery of body lower opening.
Plane residing for the edge of the upper shed of described circulating line and the plane residing for the edge of lower opening are the inclined-plane of the inclined bottom surface to liquid storage chamber, the drainage buffering mantle edge that the upper shed center to circulating line that the upper shed of circulating line is provided with extends.
Described liquid storage chamber is semielliptical shape, and the inwall of liquid storage chamber is oval arcuation, and described circulating line is two, and two circulating lines are symmetricly set on the both ends of ellipsoid major diameter respectively.
On the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, and the upper end open of appearance air chamber is provided with container cover, and the sidewall holding air chamber is provided with ventilative window, and ventilative window is provided with degerming filter membrane.
On the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, hold air chamber upper end open on sealing be provided with container cover, hold air chamber sidewall on or in container cover interval be communicated be provided with the air inlet mouth of pipe, the mouth of pipe of giving vent to anger, the liquid in-out mouth of pipe, sampling nozzle.
The bottom surface of liquid storage chamber of the present invention is smooth concave surface, the side wall surface of liquid storage chamber is slope, seamlessly transit between slope and concave surface, the sidewall of liquid storage chamber coincide along slope and is provided with circulating line, when driving the present invention to vacillate now to the left, now to the right, the nutrient solution being arranged in upper strata can constantly enter circulating line by the upper shed of two circulating lines and be gushed out by lower opening, and be originally in lower floor nutrient solution can in this process on turn over, realize the circulation up and down of nutrient solution, simultaneously, lower opening due to circulating line is provided with unidirectional venting valve structure, the liquid entering circulating line may be only supernatant liquid, circulation is effectively simple, mixing ventilation effect is good, and the frequency of circulation can drop to Schwellenwert, circulation shear power is minimum.Meanwhile, culture vessel of the present invention is hard culture vessel, moves conveniently.
Circulating line of the present invention is formed by the identical body be installed on the sidewall of liquid storage chamber, and body can separate shaping with culture vessel, and manufacturing process is better.
Unidirectional venting valve structure of the present invention is plural bag-shaped flexible valve, the sack of bag-shaped flexible valve is towards the port of lower opening, banded limit diaphragm is arranged on the inwall of circulating line by a side, wave in driving the present invention like this, when the upper shed position of circulating line reduces, nutrient solution can enter in circulating line from upper shed, the nutrient solution of lower opening will be full of bag-shaped flexible valve, bag-shaped flexible valve expands and is shut by lower opening, when the upper shed position of circulating line raises, nutrient solution extrudes bag-shaped flexible valve in circulating line, liquid in bag-shaped flexible valve is extruded, bag-shaped flexible valve smaller volume, nutrient solution will be discharged by lower opening, this valve is biomimetic features, unidirectional through-flow reliability is high.
On the opening on culture vessel top of the present invention, upwards one is extended has column to hold air chamber, the upper end open of appearance air chamber is provided with container cover, on the sidewall of appearance air chamber, interval connection is provided with inlet mouth and air outlet, when the volume of culture vessel is larger, when the oxygen that nutrient solution dissolves by means of only culture vessel upper end open place contacts with air cannot be satisfied the demand, by inlet mouth toward oxygenation in culture vessel, discharged the waste gas of organism generation simultaneously by air outlet, the vegetative activity of organism can well be ensured.
Culture vessel of the present invention is semielliptical shape, and inner chamber is oval arcuation, such circulating line arrangement convenience, and circulation fluid is movement gently back and forth on the long axis direction of oval ball, shearing force can be dropped to minimum.
Accompanying drawing explanation
Fig. 1 is the stereographic map of the embodiment of the present invention 1;
Fig. 2 is the sectional view of the front view of the embodiment of the present invention 1;
Fig. 3 is the vertical view of Fig. 2;
Fig. 4 is the sectional view of the front view of the embodiment of the present invention 2;
Fig. 5 is the vertical view of Fig. 4;
Fig. 6 is the perspective view of Fig. 5 middle tube body;
Fig. 7 is the stereographic map of the embodiment of the present invention 3;
Fig. 8 is the front view of the embodiment of the present invention 3;
Fig. 9 is the perspective view of Fig. 7 middle tube body;
Figure 10 is the stereographic map of bag-shaped flexible valve in Fig. 7.
Embodiment
A kind of embodiment 1 of waving the cyclical cell culture apparatus of driving, in Fig. 1 ~ 3, this culture apparatus comprises hard culture vessel, the top of this culture vessel is for holding air chamber 3, bottom is liquid storage chamber 4, and liquid storage chamber 4 is semielliptical shape, the inner chamber ellipsoid cambered surface of liquid storage chamber 4, hold air chamber 3 to be wholely set with liquid storage chamber 4, hold air chamber 3 and upwards extend by the upper port of liquid storage chamber 4.The both sides being relatively set with two film 6, two films 6 at the both ends of the major axis of liquid storage chamber's elliposoidal inner chamber are fixedly sticked on the wall of inner chamber chamber.Culture vessel in the present embodiment is shaping by rigid plastics, and film is soft plastics, on the chamber wall of the inner chamber of the culture vessel that film can be fixed by the mode of hot melt.The part bag of the inner chamber of liquid storage chamber near major axis two ends has been set as two body chambeies as a part for circulating line by two films 6 together with the chamber wall of the inner chamber of liquid storage chamber, the upper end of circulating line has upper shed 7 upward, and the lower end of circulating line has the lower opening 9 be positioned on the bottom surface of liquid storage chamber.The inner side of lower opening 9 is provided with bag-shaped flexible valve 11, be provided with two altogether, the sack of bag-shaped flexible valve 11 is towards the port of lower opening 9, bag top is towards the inside of torus, the side of a limit diaphragm is fixedly installed on the inner side-wall of film, and the side of another end limit diaphragm is fixedly installed on the chamber wall of inner chamber.Bag-shaped flexible valve can be semi-moon shaped also can be semielliptical shape.
The upper end open of appearance air chamber 3 is equipped with container cover 14, the sidewall of appearance air chamber is oppositely arranged two ventilative windows of mutually ventilating, ventilative window is provided with degerming filter membrane 15.
The bottom of culture vessel is provided with base 5.
A kind of embodiment 2 of waving the cyclical cell culture apparatus of driving, at Fig. 4, Fig. 5, in Fig. 6, the difference of the present embodiment and embodiment 1 is: the circulating line of the present embodiment is formed by body 10, body 10 coincide and is sticked on the sidewall of liquid storage chamber, the upper port of body 10 is upper shed 7, the lower port of body 10 is lower opening 9, the upper surface of body 10 and lower surface are the inclined-plane of the inclined bottom surface to liquid storage chamber 4, therefore upper shed 7 and lower opening 9 are oval mouth, limit diaphragm 8 is the oval lamellar body coincide with the pipe port shape of body, limit diaphragm 8 be sticked lower surface on, limit diaphragm 8 is fixed on body at one end, can relative to body open and close movement.The size of limit diaphragm 8 is greater than the internal diameter of body lower opening and is less than the external diameter of body lower opening, and the mouth of pipe of body just blocks and the end face of whole body all can not be covered by limit diaphragm 8.The edge of the lower opening of body be enlarging to male cone (strobilus masculinus), the edge of limit diaphragm 8 is the conical surface coincide with the edge of the lower opening of body.Body is flexible duct, and upper shed place of body is provided with drainage buffering mantle edge 12.Drainage buffering mantle edge 12 is arranged on the upper surface of body, and extends to the mouth of pipe.
A kind of embodiment 3 of waving the cyclical cell culture apparatus of driving, in Fig. 7 ~ 10, the difference of the present embodiment and embodiment 2 is: the inner side of the lower opening 9 of the present embodiment is provided with bag-shaped flexible valve 11, be provided with two altogether, the sack of bag-shaped flexible valve 11 is towards the port of lower opening 9, bag top is towards the inside of torus, and two packed limit diaphragms are wholely set on the inner side-wall of body near the outside of tube wall.Bag-shaped flexible valve can be semi-moon shaped also can be semielliptical shape.
The upwards extended appearance air chamber 3 having column on the opening on culture vessel top, the upper end open of appearance air chamber 3 can add container cover 14, the sidewall of appearance air chamber 3 is symmetrically arranged with inlet mouth 2 air outlet 1, inlet mouth 2 air outlet 1, all be communicated with appearance air chamber 3, the sidewall holding air chamber is also provided with liquid in-out mouth, thief hole, also pre-buriedly on the sidewall holding air chamber is equipped with confession dissolved oxygen sensor and the signal wire of pH value sensor.In use also degerming filter membrane can be set on inlet mouth 2, air outlet 3, to prevent bacterial contamination.
The material of the culture vessel of above embodiment is duroplasts, also can be pottery or other mechanically resistant materials.
The bag-shaped flexible valve of above embodiment is two, also can be more.
Above embodiment is all provided with the appearance air chamber of column, also can not arrange the appearance air chamber of column, does not simultaneously also arrange container cover, allows the air on culture vessel top provide oxygen required for microbe survival.
The inner chamber of the liquid storage chamber of the present embodiment is ellipsoid, and its inner chamber can be designed to by bottom surface is in actual use smooth concave surface, and the sidewall of liquid storage chamber is slope, any shape seamlessly transitted between slope and concave surface.
The circulation body of the present embodiment can also be one-body molded with liquid storage chamber.
The cyclical cell culture apparatus waving driving in above embodiment needs to be placed on the drive unit of generation oscillating motion in use, oscillating motion can make the cyclic motion of nutrient solution generation as shown in Fig. 2,4,8 in culture vessel, to reach the object allowing liquid circulation take a breath, even if this oscillating motion also can reach the object making nutrient solution circulate extremely gently.
The cell culture apparatus of above embodiment has when shearing force is minimum, reaches maximum mixing and gaseous interchange effect, easy and simple to handle, with low cost, can single use, without crossed contamination.
In use, the cell culture apparatus of above embodiment is placed on swing incubator or other rocking equipments, the cell culture apparatus of different size does not need the swing incubator that matches with it or other rocking equipments, and same swing incubator or other rocking equipments can complete to cultivate from laboratory scale and amplify step by step again to the demand of large scale culturing.
Claims (17)
1. one kind is waved the cyclical cell culture apparatus of driving, it is characterized in that: this culture apparatus comprises hard culture vessel, the bottom of this incubator is liquid storage chamber, the bottom surface of liquid storage chamber is smooth concave surface, the side wall surface of liquid storage chamber is slope, seamlessly transits between slope and concave surface, and the sidewall of liquid storage chamber coincide along slope is provided with circulating line, circulating line has upper shed and lower opening, and lower opening is provided with unidirectional venting valve structure; The sidewall bag that described circulating line is arranged on film on the side wall surface of liquid storage chamber and liquid storage chamber by both side edges is established and is formed; Described unidirectional venting valve structure is plural bag-shaped flexible valve, and the sack of bag-shaped flexible valve is towards the port of lower opening, and the outer side correspondence of bag-shaped flexible valve is arranged on the inwall of circulating line.
2. the cyclical cell culture apparatus waving driving according to claim 1, is characterized in that: described circulating line is soft pipeline, and bag-shaped flexible valve and circulating line are wholely set.
3. the cyclical cell culture apparatus waving driving according to claim 1, it is characterized in that: the plane residing for the edge of the upper shed of described circulating line and the plane residing for the edge of lower opening are the inclined-plane of the inclined bottom surface to liquid storage chamber, the drainage buffering mantle edge that the upper shed center to circulating line that the upper shed of circulating line is provided with extends.
4. the cyclical cell culture apparatus waving driving according to claim 1, it is characterized in that: described liquid storage chamber is semielliptical shape, the inwall of liquid storage chamber is oval arcuation, and described circulating line is two, and two circulating lines are symmetricly set on the both ends of ellipsoid major diameter respectively.
5. the cyclical cell culture apparatus waving driving according to claim 1, it is characterized in that: on the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, the upper end open of appearance air chamber is provided with container cover, the sidewall holding air chamber is provided with ventilative window, and ventilative window is provided with degerming filter membrane.
6. the cyclical cell culture apparatus waving driving according to claim 1, it is characterized in that: on the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, hold air chamber upper end open on sealing be provided with container cover, hold air chamber sidewall on or in container cover interval be communicated be provided with the air inlet mouth of pipe, the mouth of pipe of giving vent to anger, the liquid in-out mouth of pipe, sampling nozzle.
7. one kind is waved the cyclical cell culture apparatus of driving, it is characterized in that: this culture apparatus comprises hard culture vessel, the bottom of this incubator is liquid storage chamber, the bottom surface of liquid storage chamber is smooth concave surface, the side wall surface of liquid storage chamber is slope, seamlessly transits between slope and concave surface, and the sidewall of liquid storage chamber coincide along slope is provided with circulating line, circulating line has upper shed and lower opening, and lower opening is provided with unidirectional venting valve structure; Described circulating line is formed by the identical body be installed on the sidewall of liquid storage chamber; Described unidirectional venting valve structure is plural bag-shaped flexible valve, and the sack of bag-shaped flexible valve is towards the port of lower opening, and the outer side correspondence of bag-shaped flexible valve is arranged on the inwall of circulating line.
8. the cyclical cell culture apparatus waving driving according to claim 7, is characterized in that: described circulating line is soft pipeline, and bag-shaped flexible valve and circulating line are wholely set.
9. the cyclical cell culture apparatus waving driving according to claim 7, it is characterized in that: the plane residing for the edge of the upper shed of described circulating line and the plane residing for the edge of lower opening are the inclined-plane of the inclined bottom surface to liquid storage chamber, the drainage buffering mantle edge that the upper shed center to circulating line that the upper shed of circulating line is provided with extends.
10. the cyclical cell culture apparatus waving driving according to claim 7, it is characterized in that: described liquid storage chamber is semielliptical shape, the inwall of liquid storage chamber is oval arcuation, and described circulating line is two, and two circulating lines are symmetricly set on the both ends of ellipsoid major diameter respectively.
11. cyclical cell culture apparatus waving driving according to claim 7, it is characterized in that: on the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, the upper end open of appearance air chamber is provided with container cover, the sidewall holding air chamber is provided with ventilative window, and ventilative window is provided with degerming filter membrane.
12. cyclical cell culture apparatus waving driving according to claim 7, it is characterized in that: on the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, hold air chamber upper end open on sealing be provided with container cover, hold air chamber sidewall on or in container cover interval be communicated be provided with the air inlet mouth of pipe, the mouth of pipe of giving vent to anger, the liquid in-out mouth of pipe, sampling nozzle.
13. 1 kinds of cyclical cell culture apparatus waving driving, it is characterized in that: this culture apparatus comprises hard culture vessel, the bottom of this incubator is liquid storage chamber, the bottom surface of liquid storage chamber is smooth concave surface, the side wall surface of liquid storage chamber is slope, seamlessly transits between slope and concave surface, and the sidewall of liquid storage chamber coincide along slope is provided with circulating line, circulating line has upper shed and lower opening, and lower opening is provided with unidirectional venting valve structure; Described circulating line is formed by the identical body be installed on the sidewall of liquid storage chamber; Described unidirectional venting valve structure is the paddle type limit diaphragm coincide with the pipe port shape of body, and limit diaphragm folding is sticked on the lower opening of body, and the outer rim of limit diaphragm is less than the periphery of body lower opening.
14. cyclical cell culture apparatus waving driving according to claim 13, it is characterized in that: the plane residing for the edge of the upper shed of described circulating line and the plane residing for the edge of lower opening are the inclined-plane of the inclined bottom surface to liquid storage chamber, the drainage buffering mantle edge that the upper shed center to circulating line that the upper shed of circulating line is provided with extends.
15. cyclical cell culture apparatus waving driving according to claim 13, it is characterized in that: described liquid storage chamber is semielliptical shape, the inwall of liquid storage chamber is oval arcuation, and described circulating line is two, and two circulating lines are symmetricly set on the both ends of ellipsoid major diameter respectively.
16. cyclical cell culture apparatus waving driving according to claim 13, it is characterized in that: on the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, the upper end open of appearance air chamber is provided with container cover, the sidewall holding air chamber is provided with ventilative window, and ventilative window is provided with degerming filter membrane.
17. cyclical cell culture apparatus waving driving according to claim 13, it is characterized in that: on the opening on described liquid storage chamber top, upwards one is extended has column to hold air chamber, hold air chamber upper end open on sealing be provided with container cover, hold air chamber sidewall on or in container cover interval be communicated be provided with the air inlet mouth of pipe, the mouth of pipe of giving vent to anger, the liquid in-out mouth of pipe, sampling nozzle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010532912.5A CN101974424B (en) | 2010-11-05 | 2010-11-05 | Swing-driven circular cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010532912.5A CN101974424B (en) | 2010-11-05 | 2010-11-05 | Swing-driven circular cell culture device |
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| CN101974424A CN101974424A (en) | 2011-02-16 |
| CN101974424B true CN101974424B (en) | 2015-03-25 |
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| CN201010532912.5A Expired - Fee Related CN101974424B (en) | 2010-11-05 | 2010-11-05 | Swing-driven circular cell culture device |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103415610B (en) * | 2011-03-18 | 2016-10-19 | 通用电气健康护理生物科学股份公司 | For cultivating the flexible bag of cell |
| CN102618441A (en) * | 2012-03-14 | 2012-08-01 | 江苏农林职业技术学院 | Swinging type liquid intermittent submerged culture apparatus |
| CN103911285B (en) * | 2014-03-08 | 2017-03-22 | 郑州威瑞生物技术有限公司 | Disposable bioreactor |
| CN109289958B (en) * | 2017-07-24 | 2023-10-31 | 郑州威瑞生物技术有限公司 | Flip formula cap and flip formula test tube assembly |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201545840U (en) * | 2009-09-21 | 2010-08-11 | 惠识瑶 | Packed bed cell bioreactor of stirring-free device |
| CN101812405A (en) * | 2010-04-30 | 2010-08-25 | 惠倪 | Polygonal plastic bag swing cell reactor and method for culturing animal cells |
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2010
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201545840U (en) * | 2009-09-21 | 2010-08-11 | 惠识瑶 | Packed bed cell bioreactor of stirring-free device |
| CN101812405A (en) * | 2010-04-30 | 2010-08-25 | 惠倪 | Polygonal plastic bag swing cell reactor and method for culturing animal cells |
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