CN104711164A - Fermentation tank with shake flask function and fermentation method thereof - Google Patents
Fermentation tank with shake flask function and fermentation method thereof Download PDFInfo
- Publication number
- CN104711164A CN104711164A CN201510098481.9A CN201510098481A CN104711164A CN 104711164 A CN104711164 A CN 104711164A CN 201510098481 A CN201510098481 A CN 201510098481A CN 104711164 A CN104711164 A CN 104711164A
- Authority
- CN
- China
- Prior art keywords
- tank
- fermentation
- agitator
- fermentor tank
- tank body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a fermentation tank with a shake flask function and a fermentation method thereof. The fermentation tank with the shake flask function and the fermentation method thereof solve the problems that obvious difference exists between the mixing conditions of internal air of a shake flask and those of an existing fermentation tank and combined exploration of various factors such as ventilation, stirring and device on the fermentation tank is needed again. The inner wall of a tank body is fixedly connected with a baffle plate, and the radial direction of the tank body is vertical to a tank bottom; the tank body is cylinder-shaped, and is connected with an upper opening; the tank bottom inside the tank body is fixedly connected with a support; the support consists of a horizontal cross and an upright post in the center of the horizontal cross; stirrers are fixed on the upright post; the stirrers are symmetric devices respectively composed of a horizontal rod and impellers connected to two ends of the horizontal rod; bulges are formed in the outer wall of the tank body and used for fixing the tank body to a shake flask machine.
Description
technical field:
The present invention relates to biotechnology apparatus field, be specifically related to a kind of use on shaking table and be different from fermentation unit and the fermentation process of the various biological culture of triangle shaking flask.
background technology:
Liquid fermenting is one of Main Means of production Bio-engineering Products, as a big chunk microbiotic, yeast, amino acid,
Zymin and organic acid etc.In fermentation engineering, a vital link is control and the optimization of fermenting process technical parameter, it is for raising leavened prod concentration, reduce fermentation byproduct, improve fermentation production strength, reduce fermentation energy consumption, improve the trans-utilization rate of substrate, the cost of reduction product separation, purifying and environmental protection load, improve equipment use efficiency, reduce investment, and then improve the whole economic efficiency important role of fermentation industry.
The research of current zymotechnique is continued by following approach: 1) on shaking table, grope basic fermentation condition by triangle shaking flask; 2) on fermentor tank, technique amplification is carried out.Fermentor tank is not that shaking table stirs, and the electric blender of being installed by tank mouth is stirred.Problems existing is that shaking flask inner air mixing condition and fermentor tank exist notable difference, needs again to grope in the combination of the many factors such as the enterprising gas that works of fermentor tank, stirring and loading amount.Due to device-restrictive, the parallel of multiple tank batch cannot be carried out and grope, manyly to carry out by rule of thumb.
Agitator paddle is the major transducer part in whipping process, and the changes mechanical energy of rotation is the kinetic energy of fluid by it, and the Flow Field Distribution of its efficiency transformed and fluid directly affects effect and the production cost of stirring.It is very inconvenient that current existing fermentor tank will study the impact of different agitator paddle on fermentation, can only be fermented a collection of again by change impeller realize, so just cause the cycle very long and the not exclusively reproduction such as the various conditions of every batch, not even that every Batch fermentation can be successful, lack circulation ratio, thus improve research cost to a great extent.
summary of the invention:
The object of this invention is to provide a kind of structure simple, cost is low, the fermentor tank of shaking flask function that what zymotechnique was easy possess.
It is low that another object of the present invention is to provide a kind of cost, zymotechnique easy by the method possessing the fermentor tank shaking tank function and carry out liquid fermenting.
The present invention is achieved in that
The fermentor tank of tool shaking flask function of the present invention, inner tank wall is fixed with tank body radial and perpendicular to the baffle plate at the bottom of tank, tank body is round shape, be threaded with suitable for reading, be convenient to put into agitator, at the bottom of tank body inner canister, be fixed with support, support is made up of the column at planche cross frame and center thereof, column is fixed with agitator, and agitator is the symmetrical mounting that the impeller connected by horizon bar and two ends thereof is formed, and tank wall has projection.
Tank neck diameter suitable for reading is 1/3 to 1/2 of tank diameter.
Described baffle plate is 3-4 pieces, and the width of baffle plate is 1/8-1/12 with the ratio of tank body internal diameter, and adjacent screen angle is 90 degree of-120 degree.
The height of described agitator is 1/4 to 1/2 of tank body height, and agitator is two of 1 or upper and lower layout.
The horizon bar length of described agitator is 1/2-1/3 with the ratio of tank body internal diameter.
Described agitator paddle type is any one in straight leaf disc turbine type, tiltedly leaf disc turbine type, curved leaf disc turbine type, arrow leaf formula orbital, semi-circular tube and parabolic, rotary propeller type and A315 formula.
Two projections of described tank wall are arranged symmetrically with, for fixing tank body at shaking table.
The fermentor tank of apparatus shaking flask function carries out the method for liquid fermenting, fermention medium will be prepared in proportion and in fermentor tank by a certain amount of each tool shaking flask function being respectively charged into different agitator paddle, clog tank mouth with gauze and put it in autosterilization pot the sterilizing that carries out disinfection, disinfection and sterilization temperature and time are respectively 120-121 DEG C, 30min, after sterilization completes, inoculation quality percentage concentration 5% bacterial classification on super clean bench, fasten gauze with rubber and to be fixed on shaking table by the fermentor tank that tool to be shaken tank function by the projection of tank wall with rope and fermented, shaking speed is 220-250r/min, ferment 6-17 days, from second day, carry out the pH value of sampling monitoring fermented liquid every day, bacterial classification, residual sugar amount, the changing conditions of valency effect and observation mycelial growth, after having fermented, by data cases in the fermentor tank that contrasts each tool shaking flask function, summarize different agitator paddle and situation is affected on fermentation, multiple impeller-type is selected repeatedly to repeat above-mentioned experiment, finally select the paddle wheel of the suitableeest fermentation, the amplification production later for this bacterial classification provides basis, the paddle wheel of the suitableeest selected fermentation is loaded each tool to be shaken in the fermentor tank of tank function, fasten gauze with rubber and to be fixed on shaking table by the fermentor tank that tool to be shaken tank function by the projection of tank wall with rope and carry out the formal fermentative production of this bacterial classification, fermentation condition is identical with above-mentioned experiment condition.
The present invention carries out large batch of the energy on probation of agitator paddle on fermentation impact simultaneously, and process is simple, and the cycle is short, and effect is easily shown in.The present invention is during the fermentation by setting different fermentations condition, and can realize a large amount of parallel laboratory tests with batch identical conditions for sterilization, identical stirring velocity, identical Ventilation Rate, cost is low, is the data instructing enlarged experiment to provide more accurately close.
Compared with prior art, the beneficial effect that the present invention has is: fermentation flask and fermentor tank integrate by the present invention, adopts this structure construction, can qualitative examination agitator paddle and the different impact on fermentation of other parameter.Because fermentor tank structure of the present invention is simple, be easy to obtain and cost is lower.Can realize under loading amount, sterilization, air input, the identical condition of shaking speed with batch a large amount of parallel laboratory test, thus greatly shorten the cycle of the parameter selecting best agitator paddle and other impact fermentation, carry out the formal fermentative production of this bacterial classification with experiment condition, suitability is extensive.The present invention is easy to use, and structure is simple, and cost is low, and experimental period is short, and fermentation efficiency is high, quality good.
accompanying drawing illustrates:
fig. 1for fermentor tank structure of the present invention
figure.
fig. 2for supporting structure
figure.
fig. 3for straight leaf disc turbine structure
figure.
fig. 4for semi-circular tube and parabolic turbine structure
figure.
fig. 5for curved leaf disc turbine type turbine structure
figure.
fig. 6for A315 formula turbine structure
figure.
fig. 7for rotary propeller type turbine structure
figure.
fig. 8for oblique leaf disc turbine type turbine structure
figure.
fig. 9for arrow leaf formula orbital turbine structure
figure.
fig. 10 is
fig. 9overlook
figure.
embodiment:
Embodiment 1:
The projection that this fermentor tank fixes agitator and tank wall by tank body, inner tank wall perpendicular to the baffle plate at the bottom of tank, tank body inner support forms, and diameter at the bottom of tank is 8cm, and interior diameter at the bottom of tank is 75cm, tank neck diameter is 4cm, tank total height is 18cm, and tank alms bowl height is 11cm, and tank neck height is 2.5cm.Inner tank wall is perpendicular to 4 pieces, the baffle plate at the bottom of tank and mutually vertically, the width of 4 pieces of baffle plates is 8mm, and its height is identical with can body height.Tank body inner support fixes an agitator, and agitator is 4cm apart from end height in fermentor tank, and the half that stirrer diameter (for the impeller total length that horizon bar and two ends thereof connect) is internal diameter at the bottom of fermentor tank, agitator paddle diameter is 1/4 of stirrer diameter.It is any one in straight leaf disc turbine type, tiltedly leaf disc turbine type, curved leaf disc turbine type, arrow leaf formula orbital, semicanal and parabolic, rotary propeller type and A315 formula that tank body inner support fixes individual layer agitator paddle type.Agitator is reinforced in the effect of the planche cross frame of support, and cross bar four end contacts with inner tank wall, is fixed on inner tank wall;
as Fig. 2.
Baffle plate is the decoration form of 3-4 pieces and effect: 3 pieces of baffle arrangement modes are for becoming " Y " type (mutual angle 120 degree), and 4 pieces of baffle arrangement modes are cross-type (mutual angle 90 degree); Effect is that when preventing fermentor tank from shaking on shaking table, in tank body, fermented liq produces whirlpool.
Embodiment 2:
Working process is as follows: be now specifically described for filamentous fungus Glarea lozoyensis ATCC 74030.This fermentor tank is identical with embodiment 1.
Select individual layer agitator, impeller selection is straight leaf disc turbine type, tiltedly leaf disc turbine type and A315 formula, and the fermentor tank put into respectively without agitator compares experiment.To prepare fermention medium in proportion and be respectively charged in each fermentor tank by a certain amount of, clog tank mouth with gauze and put it in autosterilization pot the sterilizing that carries out disinfection, disinfection and sterilization temperature and time are respectively 121 DEG C, 30min.After sterilization completes, inoculation quality concentration 5% filamentous fungus Glarea lozoyensis ATCC 74030 on super clean bench, fastened gauze with rubber and to be fixed on shaking table by three fermentor tanks by the projection of tank wall with rope and fermented, shaking speed is 220r/min.Ferment 17 days, from second day, carry out the pH value of sampling monitoring fermented liquid, bacterial classification, residual sugar amount, valency effect every day and observe the changing conditions of mycelial growth.After having fermented, by contrasting data cases in each fermentor tank, summarizing different agitator paddle affects situation to fermentation.Select straight leaf disc turbine type, tiltedly leaf disc turbine type and A315 formula to repeat experiment respectively, finally select the paddle wheel of the suitableeest fermentation to be A315 formula.The amplification production later for its bacterial classification provides basis.
Embodiment 3:
Further illustrations is carried out again for filamentous fungus Coleophoma oleae ATCC 24520.This fermentor tank is identical with embodiment 1.Select individual layer agitator, impeller selection is curved leaf disc turbine type, arrow leaf formula orbital, semi-circular tube and parabolic and rotary propeller type, and to add without individual layer agitator fermentor tank be control experiment.To prepare fermention medium in proportion and be respectively charged in each fermentor tank by a certain amount of, clog tank mouth with gauze and put it in autosterilization pot the sterilizing that carries out disinfection, disinfection and sterilization temperature and time are respectively 120 DEG C, 30min.After sterilization completes, inoculation quality concentration 5% filamentous fungus Coleophoma oleae ATCC 24520 on super clean bench, fastened gauze with rubber and to be fixed on shaking table by fermentor tank by the projection of tank wall with rope and fermented, shaking speed is 250r/min.Ferment 6 days, from second day, carry out the pH value of sampling monitoring fermented liquid, bacterial classification, residual sugar amount, valency effect every day and observe the changing conditions of mycelial growth.After having fermented, by contrasting data cases in each fermentor tank, summarizing different agitator paddle affects situation to fermentation.Select curved leaf disc turbine type, arrow leaf formula orbital, semi-circular tube and parabolic and rotary propeller type to carry out repeating experiment respectively, finally select the paddle wheel of the suitableeest fermentation to be semi-circular tube and parabolic.The amplification production later for its bacterial classification provides basis.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (8)
1. possesses the fermentor tank of shaking flask function, it is characterized in that inner tank wall is fixed with tank body radial and perpendicular to the baffle plate at the bottom of tank, tank body is round shape, is threaded with suitable for reading, is convenient to put into agitator, support is fixed with at the bottom of tank body inner canister, support is made up of the column at planche cross frame and center thereof, and column is fixed with agitator, and agitator is the symmetrical mounting that the impeller connected by horizon bar and two ends thereof is formed, tank wall has projection, for tank body is fixed to bottle swingging machine.
2. the fermentor tank possessing shaking flask function according to claim 1, is characterized in that tank neck diameter suitable for reading is 1/3 to 1/2 of tank diameter.
3. the fermentor tank possessing shaking flask function according to claim 1, is characterized in that described baffle plate is 3-4 pieces, baffle plate
Width be 1/8-1/12 with the ratio of tank body internal diameter, adjacent screen angle is 90-120 degree.
4. the fermentor tank possessing shaking flask function according to claim 1, is characterized in that the height of described agitator is 1/4 to 1/2 of tank body height, and agitator is two of 1 or upper and lower layout.
5. the fermentor tank possessing shaking flask function according to claim 1, is characterized in that the horizon bar length of described agitator is 1/2-1/3 with the ratio of tank body internal diameter.
6. the fermentor tank possessing shaking flask function according to claim 5, is characterized in that described agitator paddle type is any one in straight leaf disc turbine type, tiltedly leaf disc turbine type, curved leaf disc turbine type, arrow leaf formula orbital, semi-circular tube and parabolic, rotary propeller type and A315 formula.
7. the fermentor tank possessing shaking flask function according to claim 1, is characterized in that two projections of described tank wall are arranged symmetrically with.
8. the method for liquid fermenting is carried out with the fermentor tank possessing shaking flask function according to claim 1, it is characterized in that to prepare fermention medium in proportion and in fermentor tank by a certain amount of each tool shaking flask function being respectively charged into different agitator paddle, clog tank mouth with gauze and put it in autosterilization pot the sterilizing that carries out disinfection, disinfection and sterilization temperature and time are respectively 120-121 DEG C, 30min, after sterilization completes, inoculation quality concentration 5% bacterial classification on super clean bench, fasten gauze with rubber and by the projection of tank wall the fermentor tank of tool shaking flask function to be fixed on shaking table with rope and fermented, shaking speed is 220-250r/min, ferment 6-17 days, from second day, carry out the pH value of sampling monitoring fermented liquid every day, bacterial classification, residual sugar amount, the changing conditions of valency effect and observation mycelial growth, after having fermented, data cases in the fermentor tank of tank function is shaken by contrasting each tool, summarize different agitator paddle and situation is affected on fermentation, multiple impeller-type is selected repeatedly to repeat above-mentioned experiment, finally select the paddle wheel of the suitableeest fermentation, the amplification production later for this bacterial classification provides basis, the paddle wheel of the suitableeest selected fermentation is loaded each tool to be shaken in the fermentor tank of tank function, fasten gauze with rubber and to be fixed on shaking table by the fermentor tank that tool to be shaken tank function by the projection of tank wall with rope and carry out the formal fermentative production of this bacterial classification, fermentation condition is identical with above-mentioned experiment condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510098481.9A CN104711164A (en) | 2015-03-06 | 2015-03-06 | Fermentation tank with shake flask function and fermentation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510098481.9A CN104711164A (en) | 2015-03-06 | 2015-03-06 | Fermentation tank with shake flask function and fermentation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104711164A true CN104711164A (en) | 2015-06-17 |
Family
ID=53410884
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510098481.9A Pending CN104711164A (en) | 2015-03-06 | 2015-03-06 | Fermentation tank with shake flask function and fermentation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104711164A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105381779A (en) * | 2015-09-01 | 2016-03-09 | 河北爱能生物科技股份有限公司 | Animal-derived membrane material processing apparatus |
| CN105667851A (en) * | 2016-03-04 | 2016-06-15 | 广西壮族自治区农业科学院生物技术研究所 | Automatic culture medium split charging device |
| CN106609247A (en) * | 2015-10-21 | 2017-05-03 | 广东东阳光药业有限公司 | Deep fermentation culture method for morel |
| CN110862921A (en) * | 2019-12-19 | 2020-03-06 | 靖江神驹容器制造有限公司 | Biological fermentation tank |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5259672A (en) * | 1989-08-17 | 1993-11-09 | University Of Leicester | Shaking table having direct electromagnet drive |
| CN202246687U (en) * | 2011-09-21 | 2012-05-30 | 南通秋之友生物科技有限公司 | Shake flask used for culture of microorganism and provided with baffle plate on inner wall |
| CN203393148U (en) * | 2013-08-26 | 2014-01-15 | 东北林业大学 | Efficient cultivation and rapid sampling shake flask device |
| CN203866306U (en) * | 2014-05-13 | 2014-10-08 | 淄博职业学院 | Shake-flask culture device |
-
2015
- 2015-03-06 CN CN201510098481.9A patent/CN104711164A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5259672A (en) * | 1989-08-17 | 1993-11-09 | University Of Leicester | Shaking table having direct electromagnet drive |
| CN202246687U (en) * | 2011-09-21 | 2012-05-30 | 南通秋之友生物科技有限公司 | Shake flask used for culture of microorganism and provided with baffle plate on inner wall |
| CN203393148U (en) * | 2013-08-26 | 2014-01-15 | 东北林业大学 | Efficient cultivation and rapid sampling shake flask device |
| CN203866306U (en) * | 2014-05-13 | 2014-10-08 | 淄博职业学院 | Shake-flask culture device |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105381779A (en) * | 2015-09-01 | 2016-03-09 | 河北爱能生物科技股份有限公司 | Animal-derived membrane material processing apparatus |
| CN105381779B (en) * | 2015-09-01 | 2017-06-13 | 河北爱能生物科技股份有限公司 | A kind of animal derived film material processing unit |
| CN106609247A (en) * | 2015-10-21 | 2017-05-03 | 广东东阳光药业有限公司 | Deep fermentation culture method for morel |
| CN106609247B (en) * | 2015-10-21 | 2019-06-25 | 广东东阳光药业有限公司 | A kind of submerged culturing method for making of hickory chick |
| CN105667851A (en) * | 2016-03-04 | 2016-06-15 | 广西壮族自治区农业科学院生物技术研究所 | Automatic culture medium split charging device |
| CN110862921A (en) * | 2019-12-19 | 2020-03-06 | 靖江神驹容器制造有限公司 | Biological fermentation tank |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104711164A (en) | Fermentation tank with shake flask function and fermentation method thereof | |
| CN106282015B (en) | Systemic circulation fluidized bed bioreactor and the method for cultivating zooblast | |
| US20160177248A1 (en) | Pivoting pressurized single use bioreactor | |
| CN106520552B (en) | A kind of biological reactor for cell culture | |
| Cheng et al. | Strengthening mass transfer of carbon dioxide microbubbles dissolver in a horizontal tubular photo-bioreactor for improving microalgae growth | |
| DK2542658T3 (en) | Method and apparatus for anaerobic fermentation | |
| US20100144022A1 (en) | Continuous Flow Bioreactor | |
| CN102021115A (en) | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells | |
| CN108018204A (en) | A kind of parallel bioreactor system | |
| CN101812404B (en) | Fluidized bed type cell reactor circulating outside tank and method for cultivating animal cells | |
| CN104893957A (en) | Continuous fermentation device capable of simulating rumen digestion | |
| CN208038422U (en) | A kind of Yoghourt fermentation system | |
| CN207108986U (en) | A kind of microalgae bactogen for preventing microalgae adherence | |
| CN108004141A (en) | Rock reactor | |
| Hang et al. | Computational fluid dynamics modeling of an inverted frustoconical shaking bioreactor for mammalian cell suspension culture | |
| Oosterhuis et al. | Design, Applications, and Development of Single‐Use Bioreactors | |
| CN101831385A (en) | Jar external circulatory packed bed type cell reactor and method for cultivating animal cell | |
| US20090325282A1 (en) | Vessels for mixing bioprocessing materials | |
| CN202786259U (en) | Bioreactor | |
| CN205576152U (en) | Desk -top zooblast bioreactor of pilot scale scale | |
| CN205821354U (en) | A kind of stirring paddle for culture plant cell tank | |
| CN204946451U (en) | A kind of Simple medicament test reactor | |
| CN110317719A (en) | Teaching visualization biological fermentation tank | |
| CN205223205U (en) | Fermentation cylinder stirring rod | |
| US20220119751A1 (en) | Impeller assembly for a bioprocessing system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150617 |
|
| WD01 | Invention patent application deemed withdrawn after publication |