CN207108986U - A kind of microalgae bactogen for preventing microalgae adherence - Google Patents
A kind of microalgae bactogen for preventing microalgae adherence Download PDFInfo
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- CN207108986U CN207108986U CN201720997116.6U CN201720997116U CN207108986U CN 207108986 U CN207108986 U CN 207108986U CN 201720997116 U CN201720997116 U CN 201720997116U CN 207108986 U CN207108986 U CN 207108986U
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- microalgae
- sieve plate
- glass shell
- discharging tube
- tube
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Abstract
The utility model discloses a kind of microalgae bactogen for preventing microalgae adherence, it is characterised in that including glass shell, the filtration combination part being positioned over inside glass shell, it is nested inside with two-stage sieve plate, and collection ware is placed with the first sieve plate, micro-strainer is fixed with the second sieve plate.The side wall of the glass shell and the distance of filtration combination component side walls are 2 5mm, and the filtration combination part is 2 5mm with the beeline of the glass shell bottom under the support of the first discharging tube.The utility model overcomes the deficiency that microalgae is easily adherent in traditional micro algae culturing device running, it is possible to achieve continuous culture, improves microdisk electrode density, saves energy consumption, it is high to repeat usability.
Description
Technical field
The utility model belongs to experimental provision and field of chemical equipment, can prevent microalgae from pasting more particularly, to one kind
The microalgae bactogen of wall.
Background technology
Micro algae growth breeding is fast, photosynthetic efficiency is high, and a large amount of CO can be fixed during light autotrophy2, fixed using microalgae big
Gas CO2It is considered as to realize one of important channel for increasing remittance emission reduction that increase carbon, which converges, moreover, microalgae is in geochemical elements circulation
Also play a significant role.In addition, microalgae is rich in the physiological activators such as protein, grease, pigment and a variety of unrighted acids,
It is the important source material source in the fields such as food, animal feed, bioenergy, medicine and fine chemistry industry, therefore, develops and utilizes micro-
Algae is one of important channel for solving environment, the energy and resource problem.
Scale, commercialized microalgae industry cultivating microalgae typically by way of manually cultivating, led in food, medicine etc.
Domain, due to needing aseptic condition and strict manual control, or use genetic engineering algae kind and High Density Cultivation, culture apparatus
Use closed photo bioreactor more.But the closed photo bioreactor manually cultivated currently used for microalgae still have with
Lower problem：
(1) due to the phototaxis of microalgae so that microalgae adherence grows, and hinders penetrating for light, influences microdisk electrode concentration
Further improve, reduce culture efficiency.
(2) conventional apparatus needs to be additionally provided power stirring, gas and nutrient solution is mixed and is promoted to homogenize, but
Add energy consumption.
(3) after microalgae adherence is serious, device needs to shut down cleaning, and the waste and culture processes for causing culture medium are difficult to reality
Now prolonged continuous operation, and frequently stop cleaning more reduces production efficiency.
Utility model content
For the drawbacks described above or Improvement requirement of prior art, the utility model provide it is a kind of can prevent it is adherent micro-
Algae bactogen, its object is to provide one kind to prevent microalgae adherence, it is not necessary to provide mixing component, promote nutrition
Liquid mixes, and can realize the culture apparatus that microalgae is continuously cultivated, and thus solves the technical problem of microdisk electrode process.
To achieve the above object, according to one side of the present utility model, there is provided a kind of microalgae for preventing microalgae adherence
Bactogen, including glass shell, rubber stopper, feed tube, probe tube, blast pipe, four-way pipe, the first discharging tube, second
Discharging tube and air inlet pipe；By rubber stopper seal at the glass shell top end opening, at bottom end opening with four-way pipe airtight connection；
Rubber stopper nested inside feed tube, probe tube and the blast pipe；First discharging tube is L-shaped, the four-way pipe level side
To the horizontal component for air inlet pipe and the first discharging tube, vertical direction is the second discharging tube；The feed tube, probe tube, first
Discharging tube, the second discharging tube and air inlet pipe carry valve；The blast pipe and air inlet pipe carry degerming filter membrane；The air inlet pipe
The distance of degerming filter membrane and four-way pipe is more than the valve of air inlet pipe and the distance of four-way pipe；Also include filtration combination part, collect
Ware, the first sieve plate, micro-strainer and the second sieve plate；The filtration combination part is placed in inside the glass shell；The filtering
The sieve plate of combiner nested inside first and the second sieve plate, the collection ware are placed on first sieve plate, the micro-strainer
It is fixed on second sieve plate；Filtration combination part (6) lower end and the vertical component of first discharging tube (12) connect
Connect；The distance of the side wall of the glass shell and the side wall of filtration combination part is 2-5mm, and the filtration combination part is first
With the beeline of the glass shell bottom it is 2-5mm under the support of discharging tube.
Preferably, the aperture of the micro-strainer is 0.45-30 μm, selects aperture to be less than according to the microalgae species of culture micro-
The micro-strainer of frustule diameter.
Preferably, the aperture of first sieve plate and the second sieve plate is 1-3mm, and two sieve plate spacing are more than 10mm.
Preferably, it is connected through a screw thread or clamp connection or silica gel hose with four-way pipe at the glass shell bottom end opening
Socket.
In general, a kind of microalgae bactogen for preventing microalgae adherence constructed by the utility model and tradition training
Foster device is compared, and is had further the advantage that while realizing continuous culture：
(1) filtration combination part is combined with air inlet pipe, forms aerator.The gas being pumped into by air inlet pipe can be in glass
Glass outer casing inner wall forms annular air curtain, and rises along glass shell inwall, washes away the microalgae being attached on glass shell, passes through
Bubble floating produces the microalgae that shearing force peels off adherent growth, so as to the permanently effective adherent growth for preventing microalgae, therefore can be with
Improve microdisk electrode density.
(2) agitating device is not used, by annular air curtain caused by air-blowing, drives nutrient solution to form circulation in a device,
Reach the effect of stirring, and be well mixed micro algae culturing liquid, while reduce reactor complexity and manufacturing cost and energy
Consumption.
(3) due to the device reduces microalgae adherence, greatly reducing the number of stop cleaning, the longer time can be carried out
Continuous operation；In addition the device reduces stirring system than conventional apparatus, reduces device complexity, therefore be easily assembled to
And dismounting, energy Rapid Cleaning, reduce the time of maintenance shut-downs, improve production efficiency.
Brief description of the drawings
Fig. 1 is the utility model structure diagram.
In all of the figs, identical reference is used for representing identical element or structure, wherein：1- glass shells,
2- rubber stoppers, 3- feed tubes, 4- probe tubes, 5- blast pipes, 6- filtration combination parts, 7- collect ware, the sieve plates of 8- first, 9- micropores
Filter screen, the sieve plates of 10- second, 11- four-way pipes, the discharging tubes of 12- first, the discharging tubes of 13- second, 14- air inlet pipe.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation
Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only explaining
The utility model, it is not used to limit the utility model.In addition, institute in each embodiment of the utility model disclosed below
As long as the technical characteristic being related to does not form conflict each other, can is mutually combined.
Embodiment 1
A kind of microalgae bactogen for preventing microalgae adherence, including glass shell 1, rubber stopper 2, feed tube 3, sampling
Pipe 4, blast pipe 5, filtration combination part 6, collect ware 7, the first sieve plate 8, the sieve plate 10 of micro-strainer 9 and second, four-way pipe 11, the
One discharging tube 12, the second discharging tube 13 and air inlet pipe 14；The internal diameter of glass shell 1 is 74mm, a length of 600mm, and wall thickness is
4mm, sealed by rubber stopper 2 at top end opening, at bottom end opening with the airtight connection of four-way pipe 11；The nested inside of rubber stopper 2
Feed tube 3, probe tube 4 and blast pipe 5, wherein, feed tube 3 and the end of blast pipe 5 and rubber stopper lower end distance 10mm, probe tube
4 ends and rubber stopper lower end distance 550mm, close to collection ware 7；First discharging tube 12 is L-shaped, and the four-way pipe 11 is horizontal
Direction is the horizontal component of the discharging tube 12 of air inlet pipe 14 and first, and vertical direction is the second discharging tube 13；The feed tube 3, take
Sample pipe 4, the first discharging tube 12, the second discharging tube 13 and air inlet pipe 14 carry valve；The blast pipe 5 and air inlet pipe 14 carry hole
Footpath is 0.22 μm of degerming filter membrane；The degerming filter membrane of the air inlet pipe 14 is more than the valve of air inlet pipe 14 with the distance of four-way pipe 11
With the distance of four-way pipe 11；The external diameter of filtration combination part 6 is 70mm, a length of 60mm, is made up of PVC plastic, inside has two
The stair-stepping flange of level, for installing the first sieve plate 8 and the second sieve plate 10；Filtration combination part 6 is placed in the glass shell 1
Portion, side wall and the side wall distance of glass shell 1 are 2mm, and are connected with the first discharging tube 12 of rigid, by discharging tube 12
Support, the beeline for making its bottom and the bottom of glass shell 1 is 2mm；The nested inside of filtration combination part 6 first is sieved
The sieve plate 10 of plate 8 and second, the aperture of sieve plate is 1mm, totally 96 hole；The collection ware 7 is fixed on first sieve plate 8, described
Micro-strainer 9 is fixed on second sieve plate 10；The lower end of filtration combination part 6 is vertical with first discharging tube 12
Part connects；The aperture of the micro-strainer 9 is 0.45-30 μm, selects aperture to be less than microalgae according to the microalgae species of culture thin
The micro-strainer of born of the same parents' diameter, such as about 3-8 μm of chlorella cells diameter, you can the micro-strainer of 0.8 μm of selection；The glass shell
It is socketed at 1 bottom end opening with four-way pipe 11 by screw thread, clamp connection or silica gel hose.
Embodiment 2
A kind of microalgae bactogen for preventing microalgae adherence, including glass shell 1, rubber stopper 2, feed tube 3, sampling
Pipe 4, blast pipe 5, filtration combination part 6, collect ware 7, the first sieve plate 8, micro-strainer 9, the second sieve plate 10, four-way pipe 11, the
One discharging tube 12, the second discharging tube 13 and air inlet pipe 14；The internal diameter of glass shell 1 is 80mm, a length of 600mm, and wall thickness is
4mm, sealed by rubber stopper 2 at top end opening, at bottom end opening with the airtight connection of four-way pipe 11；The nested inside of rubber stopper 2
Feed tube 3, probe tube 4 and blast pipe 5, wherein, feed tube 3 and the end of blast pipe 5 and rubber stopper lower end distance 10mm, probe tube
4 ends and rubber stopper lower end distance 550mm, close to collection ware 7；First discharging tube 12 is L-shaped, and the four-way pipe 11 is horizontal
Direction is the horizontal component of the discharging tube 12 of air inlet pipe 14 and first, and vertical direction is the second discharging tube 13；The feed tube 3, take
Sample pipe 4, the first discharging tube 12, the second discharging tube 13 and air inlet pipe 14 carry valve；The blast pipe 5 and air inlet pipe 14 carry hole
Footpath is 0.22 μm of degerming filter membrane；The degerming filter membrane of the air inlet pipe 14 is more than the valve of air inlet pipe 14 with the distance of four-way pipe 11
With the distance of four-way pipe 11；The external diameter of filtration combination part 6 is 70mm, a length of 60mm, is made up of PVC plastic, inside has two
The stair-stepping flange of level is used to install the first sieve plate 8 and the second sieve plate 10；Filtration combination part 6 is placed in the glass shell 1
Portion, side wall and the side wall distance of glass shell 1 are 5mm, and are connected with the first discharging tube 12 of rigid, by discharging tube 12
Support, the beeline for making its bottom and the bottom of glass shell 1 is 5mm；The nested inside of filtration combination part 6 first is sieved
The sieve plate 10 of plate 8 and second, the aperture of sieve plate is 3mm, totally 48 hole；The collection ware 7 is fixed on first sieve plate 8, described
Micro-strainer 9 is fixed on second sieve plate 10；The lower end of filtration combination part 6 is vertical with first discharging tube 12
Part connects；The aperture of the micro-strainer 9 is 0.45-30 μm, selects aperture to be less than microalgae according to the microalgae species of culture thin
The micro-strainer of born of the same parents' diameter, such as about 3-8 μm of chlorella cells diameter, you can the micro-strainer of 0.8 μm of selection；The glass shell
It is socketed at 1 bottom end opening with four-way pipe 11 by screw thread, clamp connection or silica gel hose.
Embodiment 3
A kind of microalgae bactogen for preventing microalgae adherence, including glass shell 1, rubber stopper 2, feed tube 3, sampling
Pipe 4, blast pipe 5, filtration combination part 6, collect ware 7, the first sieve plate 8, micro-strainer 9, the second sieve plate 10, four-way pipe 11, the
One discharging tube 12, the second discharging tube 13 and air inlet pipe 14；The internal diameter of glass shell 1 is 75mm, a length of 600mm, and wall thickness is
4mm, sealed by rubber stopper 2 at top end opening, at bottom end opening with the airtight connection of four-way pipe 11；The nested inside of rubber stopper 2
Feed tube 3, probe tube 4 and blast pipe 5, wherein, feed tube 3 and the end of blast pipe 5 and rubber stopper lower end distance 10mm, probe tube
4 ends and rubber stopper lower end distance 550mm, close to collection ware 7；First discharging tube 12 is L-shaped, and the four-way pipe 11 is horizontal
Direction is the horizontal component of the discharging tube 12 of air inlet pipe 14 and first, and vertical direction is the second discharging tube 13；The feed tube 3, take
Sample pipe 4, the first discharging tube 12, the second discharging tube 13 and air inlet pipe 14 carry valve；The blast pipe 5 and air inlet pipe 14 carry hole
Footpath is 0.22 μm of degerming filter membrane；The degerming filter membrane of the air inlet pipe 14 is located at valve of the distance more than air inlet pipe 14 of four-way pipe 11
Door is located at the distance of four-way pipe 11；The external diameter of filtration combination part 6 is 70mm, a length of 60mm, is made up of PVC plastic, internal
Have the stair-stepping flange of two-stage be used for install the first sieve plate 8 and the second sieve plate 10；Filtration combination part 6 is placed in the glass shell
Inside 1, side wall and the side wall distance of glass shell 1 are 2.5mm, and are connected with the first discharging tube 12 of rigid, by row
The support of liquid pipe 12, the beeline for making its bottom and the bottom of glass shell 1 is 2.5mm；The inside of filtration combination part 6 is embedding
The first sieve plate 8 and the second sieve plate 10 are covered, the aperture of sieve plate is 2mm, totally 72 hole；The collection ware 7 is fixed on first sieve plate 8
On, the micro-strainer 9 is fixed on second sieve plate 10；The lower end of filtration combination part 6 and first discharging tube
12 vertical component connection；The aperture of the micro-strainer 9 is 0.45-30 μm, selects aperture small according to the microalgae species of culture
In the micro-strainer of microalgae cell diameter, such as about 3-8 μm of chlorella cells diameter, you can the micro-strainer of 0.8 μm of selection；It is described
It is socketed at the bottom end opening of glass shell 1 with four-way pipe 11 by screw thread, clamp connection or silica gel hose.
Embodiment 4
Chlorella is carried out with described device continuously to cultivate, specific implementation step is as follows：
(1) check and ensure that all valves are closed mode；Feed tube 3 is connected with constant flow pump, opened on feed tube 3
Valve, open constant flow pump and pour into 2% formalin in device, after formalin is full of device, close constant flow pump and to enter
Valve in liquid pipe 3；Air inlet pipe 14 is connected with air pump, opens the valve in air inlet pipe 14, air pump is opened, is cleaned, disappeared
Poison and sterilizing；After 6h, the valve on closing air pump and air inlet pipe 14；By the first discharging tube 12 and the second discharging tube 13 and constant flow pump
Connection, two valves thereon are opened, after opening constant flow pump emptying thimerosal, close all valves.
(2) valve on feed tube 3 is opened, constant flow pump is opened, is poured into sterile distilled water in device by feed tube 3；
The valve in air inlet pipe 14 is opened, air pump is opened, is cleaned；Open two on the first discharging tube 12 and the second discharging tube 13
Valve, emptying cleaning fluid；Repeat the above steps 3 times；After thorough emptying cleaning fluid, open air pump and ventilated by air inlet pipe 14, band
Walk moisture make inside device rapid draing to glass shell without the obvious globule after, close all valves.
(3) valve on feed tube 3 is opened, sterilized BG-11 micro-algae culture mediums are poured into by the dress by feed tube 3
In putting, chlorella algae kind (inoculative proportion is 1000 cell/ml) is then accessed, opens the valve in air inlet pipe 14, opens gas
Pump mixes algae kind, and intensity of illumination is arranged to 5400Lux.The gas being pumped into by air inlet pipe 14 can be from filtration combination part and glass
Sprayed in slit between glass outer casing inner wall, form annular air curtain, and risen along glass shell inwall, washed away and be attached to glass
Microalgae on shell, the microalgae of adherent growth is peeled off by shearing force caused by bubble floating, prevent that the microalgae light that becomes is adherent, simultaneously
Drive nutrient solution to form circulation in a device, reach the effect of stirring, mix micro algae culturing liquid.
(4) 15d or so, chlorella, which enters, stablizes growth period, opens the constant flow pump being connected with feed tube 3, sets feed liquor
Speed be 0.02L/h, sterilized BG-11 micro-algae culture mediums are poured into by feed tube 3 and are used as feed supplement in described device；Together
When open the constant flow pump that is connected with the first discharging tube 12, the speed of discharge opeing is set as 0.02L/h, by filtration combination part
Second sieve plate in portion and the filtering of micro-strainer, the microalgae for being introduced into collection ware on a small quantity are trapped, and the waste liquid that nutrition exhausts passes through
First discharging tube 12 is discharged, to realize continuous culture.
(5) micro algae culturing liquid 5ml is periodically gathered by probe tube 4, determines chlorella cells quantity and Chlorophyll-a Content.
After 30d, when bead algae biomass has reached requirement level, the valve and air pump closed in air inlet pipe 14, close feed tube 3,
Valve and constant flow pump on first discharging tube 12,2h is stood, the microalgae collected in ware is gathered by probe tube 4.
Repeating above step can continuously be cultivated next time.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, all any modification, equivalent and improvement made within the spirit and principles of the invention etc., all should be included
Within protection scope of the present invention.
Claims (7)
1. a kind of microalgae bactogen for preventing microalgae adherence, including glass shell (1), rubber stopper (2), feed tube (3),
Probe tube (4), blast pipe (5), four-way pipe (11), the first discharging tube (12), the second discharging tube (13) and air inlet pipe (14)；It is described
Sealed at glass shell (1) top end opening by rubber stopper (2), at bottom end opening with four-way pipe (11) airtight connection；The rubber
Fill in (2) nested inside feed tube (3), probe tube (4) and blast pipe (5)；First discharging tube (12) is L-shaped；The four-way
The horizontal component that (11) horizontal direction is air inlet pipe (14) and the first discharging tube (12) is managed, vertical direction is the second discharging tube
(13)；The feed tube (3), probe tube (4), the first discharging tube (12), the second discharging tube (13) and air inlet pipe (14) carry valve
Door；The blast pipe (5) and air inlet pipe (14) carry degerming filter membrane；The degerming filter membrane of the air inlet pipe (14) and four-way pipe (11)
Distance be more than air inlet pipe (14) valve and four-way pipe (11) distance；Characterized in that, also include filtration combination part
(6) ware (7), the first sieve plate (8), micro-strainer (9) and the second sieve plate (10), are collected；The filtration combination part (6) is placed in institute
It is internal to state glass shell (1)；Filtration combination part (6) first sieve plate of nested inside (8) and the second sieve plate (10), the receipts
Collection ware (7) is placed on first sieve plate (8), and the micro-strainer (9) is fixed on second sieve plate (10)；The filtering
Combiner (6) lower end is connected with the vertical component of first discharging tube (12)；The side wall of the glass shell (1) and filtering
The distance of the side wall of combiner (6) is 2-5mm, the filtration combination part (6) under the support of the first discharging tube (12) with
The beeline of glass shell (1) bottom is 2-5mm.
2. the microalgae bactogen as claimed in claim 1 for preventing microalgae adherence, it is characterised in that the micro-strainer
(9) aperture is 0.45 μm -30 μm.
3. the microalgae bactogen as claimed in claim 2 for preventing microalgae adherence, it is characterised in that first sieve plate
(8) and the screen-aperture of the second sieve plate (10) is 1-3mm.
4. the microalgae bactogen as claimed in claim 3 for preventing microalgae adherence, it is characterised in that first sieve plate
(8) spacing between the second sieve plate (10) is more than 10mm.
5. the microalgae bactogen as claimed in claim 1 for preventing microalgae adherence, it is characterised in that the glass shell
(1) it is to be threadedly coupled with the connected mode of four-way pipe (11) at bottom end opening.
6. the microalgae bactogen as claimed in claim 1 for preventing microalgae adherence, it is characterised in that the glass shell
(1) connected mode at bottom end opening with four-way pipe (11) is clamp connection.
7. the microalgae bactogen as claimed in claim 1 for preventing microalgae adherence, it is characterised in that the glass shell
(1) connected mode at bottom end opening with four-way pipe (11) is socketed for silica gel hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720997116.6U CN207108986U (en) | 2017-08-10 | 2017-08-10 | A kind of microalgae bactogen for preventing microalgae adherence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720997116.6U CN207108986U (en) | 2017-08-10 | 2017-08-10 | A kind of microalgae bactogen for preventing microalgae adherence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207108986U true CN207108986U (en) | 2018-03-16 |
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ID=61578367
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720997116.6U Expired - Fee Related CN207108986U (en) | 2017-08-10 | 2017-08-10 | A kind of microalgae bactogen for preventing microalgae adherence |
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| Country | Link |
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| CN (1) | CN207108986U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575166A (en) * | 2020-04-23 | 2020-08-25 | 南开大学 | Utilize chlorella to absorb molybdenum disulfide reaction unit |
| CN113244664A (en) * | 2021-05-12 | 2021-08-13 | 伍俊雄 | Device for demulsifying and separating vaccine |
| CN115784214A (en) * | 2023-02-08 | 2023-03-14 | 厦门凯纳石墨烯技术股份有限公司 | Reaction device for graphene growth and graphene preparation method |
-
2017
- 2017-08-10 CN CN201720997116.6U patent/CN207108986U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575166A (en) * | 2020-04-23 | 2020-08-25 | 南开大学 | Utilize chlorella to absorb molybdenum disulfide reaction unit |
| CN111575166B (en) * | 2020-04-23 | 2021-08-06 | 南开大学 | Utilize chlorella to absorb molybdenum disulfide reaction unit |
| CN113244664A (en) * | 2021-05-12 | 2021-08-13 | 伍俊雄 | Device for demulsifying and separating vaccine |
| CN115784214A (en) * | 2023-02-08 | 2023-03-14 | 厦门凯纳石墨烯技术股份有限公司 | Reaction device for graphene growth and graphene preparation method |
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