CN203393148U - Efficient cultivation and rapid sampling shake flask device - Google Patents
Efficient cultivation and rapid sampling shake flask device Download PDFInfo
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- CN203393148U CN203393148U CN201320542659.0U CN201320542659U CN203393148U CN 203393148 U CN203393148 U CN 203393148U CN 201320542659 U CN201320542659 U CN 201320542659U CN 203393148 U CN203393148 U CN 203393148U
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Abstract
The utility model provides an efficient cultivation and rapid sampling shake flask device. The efficient cultivation and rapid sampling shake flask device comprises a bottle body, a micro-pore air filtering membrane, a vent tube, a silica gel tube, a clip, a silica gel plug, a polypropylene tube and two agitating blades, wherein the silica gel plug is arranged at an opening of the bottle body; the polypropylene tube and the vent tube are inserted into the silica gel plug; the upper end of the polypropylene tube is connected with the silica gel tube; the clip is arranged on the silica gel tube; the vent tube is internally provided with the micro-pore air filtering membrane; the lower end of the polypropylene tube is connected with the agitating blades. The outer diameter of the polypropylene tube is 5mm and the inner diameter of the polypropylene tube is 3mm; the length of each blade is 15mm, the width of each blade is 10mm and the height of each blade is 15mm; the distance between each agitating blade and the bottle bottom is 20mm. The efficient cultivation and rapid sampling shake flask device is simple in structure, reasonable in design, easy and convenient to operate and high in cultivation efficiency.
Description
Technical field
The utility model relates to a kind of high-efficient culture fast sampling shaking flask device.
Background technology
Microorganism is widely used in the fields such as medicine, food, chemical industry, agricultural.In the process of microbe research, often need to carry out continuous sampling analysis to the microorganism in shaking flask, but traditional method is, every sub-sampling all will carry out in Bechtop, not only consuming time, and every sub-sampling all can increase microbiological contamination probability, once microbiological contamination, can have a strong impact on test progress.Meanwhile, traditional shake-flask culture microorganism ，Hui center produces whirlpool, causes material and microbial profile in shaking flask inhomogeneous, thereby affects culture efficiency.
Chinese Patent Application No. 200820193142.4, provides a kind of microbial cultivation oxygen increase shaking bottle, it is characterized in that: in the inwall bottom of bottle, lay a circle card article, be provided with a plurality of blades on card article; The bottom of blade and bottle is 30 °～330° angles.Its weak point is, the card article described in this patent is movable, and in the process of shaking in shaking flask, the direction that card article also can shake with shaking flask, synchronously shakes, and does not therefore tackle the problem at its root.
Chinese Patent Application No. 201120355133.2, provides a kind of inwall to be provided with the shaking flask for microorganism culturing of baffle plate, it is characterized in that, one end of described baffle plate is fixed on bottle Nei bottom, and is connected with bottle.Its weak point is, the baffle plate described in this patent is fixed on bottle wall, therefore, shaking flask is cleaned to Shi You dead angle, cannot carry out secondary use.
Utility model content
The purpose of this utility model is to solve existing problem, and a kind of high-efficient culture fast sampling shaking flask device is provided, and to solve in Experiment on Microbiology in traditional sampling method, the problem of the loaded down with trivial details easy microbiological contamination of sampling process, can improve the culture efficiency of microorganism simultaneously.
The purpose of this utility model is achieved in that a kind of high-efficient culture fast sampling shaking flask device, comprise bottle, micropore air filtering membrane, ventpipe, silicone tube, clip, silica gel plug, polypropylene tube and agitating vane, the opening part of bottle is provided with silica gel plug, on silica gel plug, be inserted with polypropylene tube and ventpipe, polypropylene tube upper end is connected with silicone tube, silicone tube is provided with clip, is provided with micropore air filtering membrane in ventpipe, and polypropylene tube lower end is connected with agitating vane.
The utility model also has some technical characterictics like this: described polypropylene tube external diameter is 5mm, and internal diameter is 3mm, and described agitating vane is two, the long 15mm of each blade, and wide 10mm, high 15mm, agitating vane is 20mm apart from the distance at the bottom of bottle.
The utility model is simple in structure, reasonable in design, easy and simple to handle, and culture efficiency is high.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Embodiment
Below in conjunction with Figure of description, the utility model is described in further detail:
Embodiment 1
In conjunction with Fig. 1, set forth cultivation and the sampling of Cordyceps militaris (L.) Link. mycelia
1, in high-efficient culture fast sampling shaking flask device, add 250ml PDA enriched medium, 121 ℃ of sterilizing 20min in high-pressure sterilizing pot.
2, sterilized high-efficient culture fast sampling shaking flask device is taken in Bechtop and inoculated.
3, the high-efficient culture fast sampling shaking flask device of inoculating Cordyceps militaris (L.) Link. mycelia is put into turn/min of shaking table 120,25 ℃ of cultivations.
4, shaking flask is taken out to shaking table, syringe is inserted to the silicone tube 6 outside bottle 1, with being evacuated in syringe holder pipe, open the clip 5 on silicone tube 6, the nutrient solution that now vacuum makes is inhaled in syringe, clamps clip 5, takes off syringe, and sampling completes.
While 5, again sampling, syringe is inserted in the silicone tube 6 outside bottle 1, with being evacuated in syringe holder pipe, open clip, the nutrient solution remaining in during previous sample in the polypropylene tube 7 of sizing belt stirrer flows into syringe, clamps clip 5, liquid in syringe is abandoned, syringe is inserted to silicone tube 6 again, being evacuated in pipe, open the clip 5 on silicone tube 6, the nutrient solution that vacuum makes is inhaled in syringe, clamp clip 5, take off syringe, sampling completes.
The effect that high-efficient culture fast sampling shaking flask device reaches:
This high-efficient culture fast sampling shaking flask device makes the sampling of microorganism in Bechtop, to carry out, but can carry out in any place, has also saved the time of operation when having guaranteed to sample not microbiological contamination; Overcome the shortcoming of the easy microbiological contamination of traditional sampling method and complex operation; Improved the convenience of sampling process; During cultivation, can effectively improve culture efficiency.
For evaluating the effect of high-efficient culture fast sampling shaking flask device, use the utility model to carry out culture experiment to Cordyceps militaris (L.) Link. mycelia, and sampling.According to test design, Cordyceps militaris (L.) Link. mycelia is inoculated in the substratum of common shaking flask and high-efficient culture fast sampling shaking flask device to each 100 bottles.In shaking table, cultivate seven days, carry out sampling operation every day, the sampling of common shaking flask will be carried out sterilizing to bleeding iron and Bechtop before operation, observes the microbiological contamination situation in shaking flask, and the dry weight that finally weighs every bottle of mycelia is calculated mean value.The results are shown in Table 1.
Table 1 high-efficient culture fast sampling shaking flask device and common medicine bottle statistical effect
Claims (2)
1. a high-efficient culture fast sampling shaking flask device, comprise bottle (1), micropore air filtering membrane (2), ventpipe (3), silicone tube (4), clip (5), silica gel plug (6), polypropylene tube (7) and agitating vane (8), it is characterized in that: the opening part of bottle (1) is provided with silica gel plug (6), on silica gel plug (6), be inserted with polypropylene tube (7) and ventpipe (3), polypropylene tube (7) upper end is connected with silicone tube (4), silicone tube (4) is provided with clip (5), in ventpipe (3), be provided with micropore air filtering membrane (2), polypropylene tube (7) lower end is connected with agitating vane (8).
2. high-efficient culture fast sampling shaking flask device according to claim 1, it is characterized in that: described polypropylene tube (7) external diameter is 5mm, internal diameter is 3mm, described agitating vane (8) is two, the long 15mm of each blade, wide 10mm, high 15mm, agitating vane (8) is 20mm apart from the distance at the bottle end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320542659.0U CN203393148U (en) | 2013-08-26 | 2013-08-26 | Efficient cultivation and rapid sampling shake flask device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320542659.0U CN203393148U (en) | 2013-08-26 | 2013-08-26 | Efficient cultivation and rapid sampling shake flask device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203393148U true CN203393148U (en) | 2014-01-15 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320542659.0U Expired - Fee Related CN203393148U (en) | 2013-08-26 | 2013-08-26 | Efficient cultivation and rapid sampling shake flask device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203393148U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711164A (en) * | 2015-03-06 | 2015-06-17 | 成都大学 | Fermentation tank with shake flask function and fermentation method thereof |
| CN114246106A (en) * | 2021-11-15 | 2022-03-29 | 江苏品品鲜生物科技有限公司 | Method for crushing velvet antler mushroom strains |
-
2013
- 2013-08-26 CN CN201320542659.0U patent/CN203393148U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104711164A (en) * | 2015-03-06 | 2015-06-17 | 成都大学 | Fermentation tank with shake flask function and fermentation method thereof |
| CN114246106A (en) * | 2021-11-15 | 2022-03-29 | 江苏品品鲜生物科技有限公司 | Method for crushing velvet antler mushroom strains |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140115 Termination date: 20140826 |
|
| EXPY | Termination of patent right or utility model |