CN103039269A - Simple liquid strain inoculation device - Google Patents
Simple liquid strain inoculation device Download PDFInfo
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- CN103039269A CN103039269A CN2012105671535A CN201210567153A CN103039269A CN 103039269 A CN103039269 A CN 103039269A CN 2012105671535 A CN2012105671535 A CN 2012105671535A CN 201210567153 A CN201210567153 A CN 201210567153A CN 103039269 A CN103039269 A CN 103039269A
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- liquid strain
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- vial
- inoculation
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Abstract
The invention relates to an edible fungus producing apparatus, in particular to a simple liquid strain inoculation device which particularly structurally comprises a glass bottle, an air pump, an inflating gun, an air inlet pipeline, a bottle stopper and a liquid strain circulating pipeline. The air pump is connected with the air inlet pipeline by a rubber pipe and air filters, the inflating gun is connected with the liquid strain circulating pipeline by a rubber pipe, the bottle stopper is fixed into a bottle opening of the glass bottle, and the air inlet pipeline and the liquid strain circulating pipeline respectively penetrate through the bottle stopper and are arranged inside the glass bottle. During inoculation, liquid strain in the bottle is pressed out of the liquid strain circulating pipeline under the action of air pressure, and is connected into culture materials under a control effect of the inflating gun. The simple liquid strain inoculation device is simple in structure, is efficient and speedy and is high in inoculation efficiency when applied to liquid strain inoculation, and is extremely low in infection rate.
Description
Technical field
The present invention relates to a kind of Edible Fungi apparatus, be specifically related to a kind of simple liquid bacterial classification inoculation apparatus.
Background technology
That edible fungi liquid strain has is with short production cycle, the cell age neat and consistent, inoculation is convenient, fermentation is fast, be suitable for the advantage such as batch production production, and its China's Edible Fungi that thoroughly is through with has been continued to use more than one thousand years and adopted the history of solid spawn, is a qualitative leap.Produce at present liquid spawn mainly be the fermentation tank culture that involves great expense out.The fermentation tank volume is large, and strain cultivation well is difficult to move connect continuously afterwards.Culture device for liquid strain of edible fungus (the patent No.: ZL200920154835.7) of my invention, utilize vial to be container, by rotary shaking table with the strain cultivation in the vial out, the vial volume is little, and easily mobile inoculation installs in other containers but must divide the liquid spawn in the bottle when inoculating, with syringe liquid spawn is received in the composts or fertilisers of cultivating again, operation is more loaded down with trivial details, and inoculation efficiency is low, and infection rate is higher.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and brand-new a kind of simple liquid bacterial classification inoculation apparatus is provided.Specifically comprise: the structure of this device is comprised of vial, air pump, charge gun, admission line, bottle stopper, liquid spawn circulation duct; Wherein, air pump and admission line are to couple together by sebific duct and air cleaner, charge gun and liquid spawn circulation duct are to couple together by sebific duct, and bottle stopper is to be fixed in the bottleneck of vial, and admission line and liquid spawn circulation duct are to pass respectively bottle stopper and be placed in the vial.During inoculation, utilize air pressure that the liquid spawn in the bottle is extruded the liquid spawn circulation duct and by charge gun control cooking cultivated in the liquid spawn access.Although the present invention is simple in structure, efficient when being applied to liquid-spawn inoculation, quick, inoculation efficiency is high, and infection rate is very low.
The present invention is achieved through the following technical solutions:
1, a kind of simple liquid bacterial classification inoculation apparatus, the structure of this device is comprised of vial (1), air pump (2), charge gun (3), admission line (4), bottle stopper (5), liquid spawn circulation duct (6) in the technical scheme; Wherein, air pump (2) and admission line (4) are to couple together by sebific duct (7) and air cleaner (8), charge gun (3) and liquid spawn circulation duct (6) are to couple together by sebific duct (9), bottle stopper (5) is to be fixed in the bottleneck of vial (1), and admission line (4) and liquid spawn circulation duct (6) are to pass respectively bottle stopper (5) and be placed in the vial (1).
1, described a kind of simple liquid bacterial classification inoculation apparatus, the vial in the technical scheme (1) is cylinder shape, is the commercial goods, can bear in high temperature, high-pressure bottle and sterilize.
3, described a kind of simple liquid bacterial classification inoculation apparatus, air pump in the technical scheme (2) is the commercial goods, be connected 2 with air pump) filter (8) that connects is a ball-type glass tube, in ball-type glass tube the inside plug cotton, the sebific duct (7) that is used for connection air pump (2) and admission line (4) can bear high temperature, autoclave sterilization.
4, described a kind of simple liquid bacterial classification inoculation apparatus, charge gun in the technical scheme (3) is the commercial goods, the sebific duct (9) that is used for connection charge gun (3) and liquid spawn circulation duct (6) can bear high temperature, autoclave sterilization.
Compared with prior art, the invention has the beneficial effects as follows:
1, the present invention is simple in structure, but efficient when being applied to liquid-spawn inoculation, quick, effectively enhances productivity.
2, use the present invention and connect liquid spawn, can be in the arbitrarily mobile inoculation of any suitable occasion.
When 3, enforcement was of the present invention, liquid spawn was arbitrarily controlled inoculum concentration under the control of charge gun, and one colludes the air gun trigger during inoculation, bacterial classification just penetrates from air gun and receives in the composts or fertilisers of cultivating, and the liquid spawn of gushing out diffusion is wide, and inoculation efficiency is high, and infection rate is very low.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is structural representation of the present invention.
In Fig. 1, the 1st, vial, the 2nd, air pump, the 3rd, charge gun, the 4th, admission line, the 5th, bottle stopper, the 6th, liquid spawn circulation duct, the 7th, the sebific duct of connection air pump and admission line, the 8th, air cleaner, the 9th, the sebific duct of connection charge gun and liquid spawn circulation duct.
Embodiment
Now the present invention will be described in detail by reference to the accompanying drawings:
The present invention is comprised of vial (1), air pump (2), charge gun (3), admission line (4), bottle stopper (5), liquid spawn circulation duct (6).
In Fig. 1, the 1st, vial (1), the 2nd, air pump (2), the 3rd, charge gun (3), the 4th, admission line (4), the 5th, bottle stopper (5), the 6th, liquid spawn circulation duct (6), the 7th, the sebific duct (7) of connection air pump and admission line, the 8th, air cleaner (8), the 9th, the sebific duct (9) of connection charge gun and liquid spawn circulation duct.Wherein, air pump (2) and admission line (4) are to couple together by sebific duct (7) and air cleaner (8), charge gun (3) and liquid spawn circulation duct (6) are to couple together by sebific duct (9), bottle stopper (5) is to be fixed in the bottleneck of vial (1), and admission line (4) and liquid spawn circulation duct (6) are to pass respectively bottle stopper (5) and be placed in the vial (1).Simultaneously, vial (1) is cylinder shape, is the commercial goods, can bear in high temperature, high-pressure bottle and sterilize; Air pump (2) is the commercial goods, be connected 2 with air pump) filter (8) that connects is a ball-type glass tube, in ball-type glass tube the inside plug cotton, the sebific duct (7) that is used for connection air pump (2) and admission line (4) can bear high temperature, autoclave sterilization; Charge gun (3) is the commercial goods, and the sebific duct (9) that is used for connection charge gun (3) and liquid spawn circulation duct (6) can bear high temperature, autoclave sterilization.
Application method of the present invention and process: first admission line (4), sebific duct (7) and air cleaner (8) are coupled together, simultaneously charge gun (3), liquid spawn circulation duct (6) and sebific duct (9) are coupled together; Then admission line (4) and liquid spawn circulation duct (6) are passed respectively bottle stopper (5) and connect into an inoculation system, then be positioned in the disinfecting container and carry out disinfection.Strain cultivation in the vial well after, be installed in the vial that liquid spawn is housed at lower sterile inoculation system of gnotobasis, admission line (4) and air pump (2) are coupled together, booster air pump, liquid spawn in the bottle is extruded liquid spawn circulation duct (6) under air pressure, by charge gun control cooking is cultivated in the liquid spawn access.
Claims (4)
1. simple liquid bacterial classification inoculation apparatus, it is characterized in that: the structure of this device is comprised of vial (1), air pump (2), charge gun (3), admission line (4), bottle stopper (5), liquid spawn circulation duct (6); Wherein, air pump (2) and admission line (4) are to couple together by sebific duct (7) and air cleaner (8), charge gun (3) and liquid spawn circulation duct (6) are to couple together by sebific duct (9), bottle stopper (5) is to be fixed in the bottleneck of vial (1), and admission line (4) and liquid spawn circulation duct (6) are to pass respectively bottle stopper (5) and be placed in the vial (1).
2. a kind of simple liquid bacterial classification inoculation apparatus described in according to claim 1, it is characterized in that: vial (1) is cylinder shape, is the commercial goods, can bear in high temperature, high-pressure bottle and sterilize.
3. a kind of simple liquid bacterial classification inoculation apparatus described in according to claim 1, it is characterized in that: air pump (2) is the commercial goods, be connected 2 with air pump) filter (8) that connects is a ball-type glass tube, in ball-type glass tube the inside plug cotton, the sebific duct (7) that is used for connection air pump (2) and admission line (4) can bear high temperature, autoclave sterilization.
4. a kind of simple liquid bacterial classification inoculation apparatus described in according to claim 1, it is characterized in that: charge gun (3) is the commercial goods, and the sebific duct (9) that is used for connection charge gun (3) and liquid spawn circulation duct (6) can bear high temperature, autoclave sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105671535A CN103039269A (en) | 2012-12-24 | 2012-12-24 | Simple liquid strain inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105671535A CN103039269A (en) | 2012-12-24 | 2012-12-24 | Simple liquid strain inoculation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103039269A true CN103039269A (en) | 2013-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105671535A Pending CN103039269A (en) | 2012-12-24 | 2012-12-24 | Simple liquid strain inoculation device |
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| CN (1) | CN103039269A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103382440A (en) * | 2013-06-24 | 2013-11-06 | 江苏元洲生物工程有限公司 | Safe inoculation device of liquid fermentation tank |
| CN105165391A (en) * | 2015-08-18 | 2015-12-23 | 何翔 | Device using air pressure to realize liquid spawn inoculation |
| CN107094495A (en) * | 2017-05-04 | 2017-08-29 | 湖南农业大学 | The production method and device of a kind of one-shot forming bacterium base |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201398322Y (en) * | 2009-04-10 | 2010-02-10 | 江苏达森发酵设备工程有限公司 | Automatic pressure maintaining device for edible fungi liquid seed production fermenter |
| CN102047813A (en) * | 2010-10-20 | 2011-05-11 | 天津渔阳酒业有限责任公司 | Sterilization and inoculation process for edible fungus culture material |
| CN102550291A (en) * | 2012-01-18 | 2012-07-11 | 山西强盛种业有限公司 | Edible fungus liquid strain inoculation and injection gun |
| CN102783356A (en) * | 2012-06-28 | 2012-11-21 | 江苏华绿生物科技股份有限公司 | Method for converting edible mushroom solid strain into liquid strain |
-
2012
- 2012-12-24 CN CN2012105671535A patent/CN103039269A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201398322Y (en) * | 2009-04-10 | 2010-02-10 | 江苏达森发酵设备工程有限公司 | Automatic pressure maintaining device for edible fungi liquid seed production fermenter |
| CN102047813A (en) * | 2010-10-20 | 2011-05-11 | 天津渔阳酒业有限责任公司 | Sterilization and inoculation process for edible fungus culture material |
| CN102550291A (en) * | 2012-01-18 | 2012-07-11 | 山西强盛种业有限公司 | Edible fungus liquid strain inoculation and injection gun |
| CN102783356A (en) * | 2012-06-28 | 2012-11-21 | 江苏华绿生物科技股份有限公司 | Method for converting edible mushroom solid strain into liquid strain |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103382440A (en) * | 2013-06-24 | 2013-11-06 | 江苏元洲生物工程有限公司 | Safe inoculation device of liquid fermentation tank |
| CN105165391A (en) * | 2015-08-18 | 2015-12-23 | 何翔 | Device using air pressure to realize liquid spawn inoculation |
| CN107094495A (en) * | 2017-05-04 | 2017-08-29 | 湖南农业大学 | The production method and device of a kind of one-shot forming bacterium base |
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Application publication date: 20130417 |
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