CN105165391A - Device using air pressure to realize liquid spawn inoculation - Google Patents

Device using air pressure to realize liquid spawn inoculation Download PDF

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Publication number
CN105165391A
CN105165391A CN201510505505.8A CN201510505505A CN105165391A CN 105165391 A CN105165391 A CN 105165391A CN 201510505505 A CN201510505505 A CN 201510505505A CN 105165391 A CN105165391 A CN 105165391A
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China
Prior art keywords
liquid spawn
air pump
air
inoculation
vial
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Pending
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CN201510505505.8A
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Chinese (zh)
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何翔
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Individual
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Individual
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Priority to CN201510505505.8A priority Critical patent/CN105165391A/en
Publication of CN105165391A publication Critical patent/CN105165391A/en
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A device using air pressure to realize liquid spawn inoculation specifically comprises a glass bottle, an air pump, an inflation gun, an air inlet pipe, a bottle stopper, and a liquid spawn circulation pipe; the air pump and the air inlet pipe are connected through a rubber pipe and an air filter; the inflation gun and the liquid spawn circulation pipe is connected through a rubber pipe; the bottle stopper is fixed in a bottleneck of the glass bottle; the air inlet pipe and the liquid spawn circulation pipe respectively penetrate the bottle stopper and are arranged in the glass bottle; in inoculation, the liquid spawn in the bottle can be pressed by the air pressure so as to enter the liquid spawn circulation pipe, and the liquid spawn is controlled by the inflation gun so as to realize inoculation and cultivation. The device is simple in structure, highly efficient and fast in liquid spawn inoculation, high in inoculation efficiency, and very low in infection rate.

Description

A kind of air pressure that utilizes is to realize the device of liquid-spawn inoculation
Technical field
The present invention relates to a kind of Edible Fungi apparatus, be specifically related to a kind of air pressure that utilizes to realize the device of liquid-spawn inoculation.
Background technology
Edible fungi liquid strain has with short production cycle, cell age neat and consistent, inoculation is convenient, fermentation is fast, be suitable for the advantages such as factorial praluction, and it thoroughly finishes China's Edible Fungi and has continued to use the history that more than one thousand years adopts solid spawn, is a qualitative leap.The fermentation tank culture mainly involved great expense of current production liquid spawn out.Fermenter volume is large, and strain cultivation is well difficult to move connect continuously afterwards.The culture device for liquid strain of edible fungus (patent No.: ZL200920154835.7) of my invention, utilize vial for container, by rotary shaker by the strain cultivation in vial out, vial volume is little, easily mobile inoculation, but the liquid spawn in bottle must be dispensed in other containers during inoculation, with syringe, liquid spawn is received in composts or fertilisers of cultivating again, operation is more loaded down with trivial details, and inoculation efficiency is low, and infection rate is higher.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art and provide brand-new a kind of air pressure that utilizes to realize the device of liquid-spawn inoculation.Specifically comprise: the structure of this device is made up of vial, air pump, charge gun, admission line, bottle stopper, liquid spawn circulation duct; Wherein, air pump and admission line are coupled together by sebific duct and air cleaner, charge gun and liquid spawn circulation duct are coupled together by sebific duct, and bottle stopper is fixed in the bottleneck of vial, and admission line and liquid spawn circulation duct are each passed through bottle stopper and are placed in vial.
The present invention is achieved through the following technical solutions:
Utilize air pressure to realize a device for liquid-spawn inoculation, be in technical scheme: the structure of this inoculator is made up of vial (1), air pump (2), charge gun (3), admission line (4), bottle stopper (5), liquid spawn circulation duct (6); Wherein, air pump (2) and admission line (4) are coupled together by sebific duct (7) and air cleaner (8), charge gun (3) and liquid spawn circulation duct (6) are coupled together by sebific duct (9), bottle stopper (5) is fixed in the bottleneck of vial (1), and admission line (4) and liquid spawn circulation duct (6) are each passed through bottle stopper (5) and are placed in vial (1).
Described vial (1) its body is cylinder shape, can bear and sterilize in high temperature, high-pressure bottle.
Described air pump (2) is a kind of " air pump ", is from an enclosure space deaeration or a kind of device adding air from enclosure space.
Described filter (8) is a ball-type glass tube, inside ball-type glass tube, fill in cotton, for connecting the sebific duct (7) in air pump (2) and admission line (4), can bear high temperature, autoclave sterilization.
Described charge gun (3) is a kind of apparatus controlling throughput.
Described can bear high temperature, autoclave sterilization for the sebific duct (9) connected in charge gun (3) and liquid spawn circulation duct (6).
The invention has the beneficial effects as follows:
1, structure of the present invention is simple, but efficient when being applied to liquid-spawn inoculation, quick, effectively enhances productivity.
2, apply the present invention and connect liquid spawn, can in the arbitrarily mobile inoculation of any suitable occasion.
When 3, implementing of the present invention, liquid spawn arbitrarily controls inoculum concentration under the control of charge gun, and during inoculation, one hooks air gun trigger, bacterial classification just penetrates from air gun to be received in composts or fertilisers of cultivating, and the liquid spawn diffusion of gushing out is wide, inoculation efficiency is high, and infection rate is very low.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is structural representation of the present invention.
In FIG, 1 is vial, and 2 is air pumps, and 3 is charge guns, and 4 is admission lines, and 5 is bottle stoppers, and 6 is liquid spawn circulation ducts, and 7 is the sebific ducts connecting air pump and admission line, and 8 is air cleaners, and 9 is the sebific ducts connecting charge gun and liquid spawn circulation duct.
Embodiment
Now the present invention will be described in detail by reference to the accompanying drawings:
The present invention is made up of vial (1), air pump (2), charge gun (3), admission line (4), bottle stopper (5), liquid spawn circulation duct (6).
In FIG, 1 is vial (1), 2 is air pump (2), 3 is charge gun (3), and 4 is admission line (4), and 5 is bottle stopper (5), 6 is liquid spawn circulation duct (6), 7 is the sebific ducts (7) connecting air pump and admission line, and 8 is air cleaner (8), and 9 is the sebific ducts (9) connecting charge gun and liquid spawn circulation duct.Wherein, air pump (2) and admission line (4) are coupled together by sebific duct (7) and air cleaner (8), charge gun (3) and liquid spawn circulation duct (6) are coupled together by sebific duct (9), bottle stopper (5) is fixed in the bottleneck of vial (1), and admission line (4) and liquid spawn circulation duct (6) are each passed through bottle stopper (5) and are placed in vial (1).Meanwhile, vial (1) its body is cylinder shape, can bear and sterilize in high temperature, high-pressure bottle; Air pump (2) is a kind of " air pump ", from an enclosure space deaeration or a kind of device adding air from enclosure space, the filter (8) be connected with air pump (2) is a ball-type glass tube, inside ball-type glass tube, fill in cotton, high temperature, autoclave sterilization can be born for the sebific duct (7) connecting air pump (2) and admission line (4); Charge gun (3) is a kind of apparatus controlling throughput, can bear high temperature, autoclave sterilization for the sebific duct (9) connecting charge gun (3) and liquid spawn circulation duct (6).
Application method of the present invention and process: first admission line (4), sebific duct (7) and air cleaner (8) are coupled together, couple together charge gun (3), liquid spawn circulation duct (6) and sebific duct (9) simultaneously; Then admission line (4) and liquid spawn circulation duct (6) are each passed through bottle stopper (5) and connect into an inoculation system, be then positioned in disinfecting container and carry out disinfection.Strain cultivation in vial well after, in an aseptic environment sterile inoculation system being installed on is equipped with in the vial of liquid spawn, admission line (4) and air pump (2) are coupled together, booster air pump, liquid spawn in bottle extrudes liquid spawn circulation duct (6) under pneumatic pressure, is controlled liquid spawn to access to cultivate cooking by charge gun.

Claims (1)

1. utilize air pressure to realize a device for liquid-spawn inoculation, it is characterized in that: the structure of this device is made up of vial (1), air pump (2), charge gun (3), admission line (4), bottle stopper (5), liquid spawn circulation duct (6); Wherein, air pump (2) and admission line (4) are coupled together by sebific duct (7) and air cleaner (8), charge gun (3) and liquid spawn circulation duct (6) are coupled together by sebific duct (9), bottle stopper (5) is fixed in the bottleneck of vial (1), and admission line (4) and liquid spawn circulation duct (6) are each passed through bottle stopper (5) and are placed in vial (1);
Described vial (1) its body is cylinder shape, can bear and sterilize in high temperature, high-pressure bottle;
Described air pump (2) is a kind of " air pump ", is from an enclosure space deaeration or a kind of device adding air from enclosure space;
Described filter (8) is a ball-type glass tube, inside ball-type glass tube, fill in cotton, for connecting the sebific duct (7) in air pump (2) and admission line (4), can bear high temperature, autoclave sterilization;
Described charge gun (3) is a kind of apparatus controlling throughput;
Described can bear high temperature, autoclave sterilization for the sebific duct (9) connected in charge gun (3) and liquid spawn circulation duct (6).
CN201510505505.8A 2015-08-18 2015-08-18 Device using air pressure to realize liquid spawn inoculation Pending CN105165391A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510505505.8A CN105165391A (en) 2015-08-18 2015-08-18 Device using air pressure to realize liquid spawn inoculation

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Application Number Priority Date Filing Date Title
CN201510505505.8A CN105165391A (en) 2015-08-18 2015-08-18 Device using air pressure to realize liquid spawn inoculation

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CN105165391A true CN105165391A (en) 2015-12-23

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2052771U (en) * 1989-04-03 1990-02-14 侯建华 Simple liquid strain production device
JP2002354940A (en) * 2001-05-31 2002-12-10 Nippon Seiki Co Ltd Apparatus for inoculating liquid spawn
JP2006204136A (en) * 2005-01-26 2006-08-10 Taiwan Agricultural Chemicals & Toxic Substances Research Inst Council Of Agriculture Automated mass-production system for liquid state inoculation
CN102550291A (en) * 2012-01-18 2012-07-11 山西强盛种业有限公司 Edible fungus liquid strain inoculation and injection gun
CN202819046U (en) * 2012-09-12 2013-03-27 崔学恒 Liquid strain high-pressure inoculator
CN103039269A (en) * 2012-12-24 2013-04-17 何寒 Simple liquid strain inoculation device
CN203057903U (en) * 2012-12-24 2013-07-17 何寒 Simple liquid strain inoculation device
CN103766141A (en) * 2014-02-17 2014-05-07 太原市福米嘉精密机械有限公司 Liquid edible fungi inoculation injection gun
CN204907260U (en) * 2015-08-18 2015-12-30 何翔 Utilize atmospheric pressure to realize device of liquid spawn inoculation

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2052771U (en) * 1989-04-03 1990-02-14 侯建华 Simple liquid strain production device
JP2002354940A (en) * 2001-05-31 2002-12-10 Nippon Seiki Co Ltd Apparatus for inoculating liquid spawn
JP2006204136A (en) * 2005-01-26 2006-08-10 Taiwan Agricultural Chemicals & Toxic Substances Research Inst Council Of Agriculture Automated mass-production system for liquid state inoculation
CN102550291A (en) * 2012-01-18 2012-07-11 山西强盛种业有限公司 Edible fungus liquid strain inoculation and injection gun
CN202819046U (en) * 2012-09-12 2013-03-27 崔学恒 Liquid strain high-pressure inoculator
CN103039269A (en) * 2012-12-24 2013-04-17 何寒 Simple liquid strain inoculation device
CN203057903U (en) * 2012-12-24 2013-07-17 何寒 Simple liquid strain inoculation device
CN103766141A (en) * 2014-02-17 2014-05-07 太原市福米嘉精密机械有限公司 Liquid edible fungi inoculation injection gun
CN204907260U (en) * 2015-08-18 2015-12-30 何翔 Utilize atmospheric pressure to realize device of liquid spawn inoculation

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Application publication date: 20151223

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