JP2006204136A - Automated mass-production system for liquid state inoculation - Google Patents

Automated mass-production system for liquid state inoculation Download PDF

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JP2006204136A
JP2006204136A JP2005018465A JP2005018465A JP2006204136A JP 2006204136 A JP2006204136 A JP 2006204136A JP 2005018465 A JP2005018465 A JP 2005018465A JP 2005018465 A JP2005018465 A JP 2005018465A JP 2006204136 A JP2006204136 A JP 2006204136A
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bottle
liquid state
culture bottle
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bacterial culture
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Suisei Ko
▲穂▼生 高
Yusho Sai
勇勝 蔡
Sekigen Rin
石源 林
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TAIWAN AGRICULTURAL CHEMICALS
Taiwan Agricultural Chemicals & Toxic Substances Research Inst Council Of Agriculture
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TAIWAN AGRICULTURAL CHEMICALS
Taiwan Agricultural Chemicals & Toxic Substances Research Inst Council Of Agriculture
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube

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Abstract

<P>PROBLEM TO BE SOLVED: To provide an automated mass-production system for the liquid-state inoculation capable of automatically inoculating a bacterial strain in liquid state. <P>SOLUTION: The mass-production system is provided with a chamber 1 having a holding space 11 and furnished with a filter set 2 for a fan, an inlet port 111, an outlet port 112 and a sterilizing lamp 12, a transfer mechanism 3 to transfer a bacteria culture bottle, a cap opening and closing mechanism having a rail, a swinging table and an air cylinder and performing the opening and closing operation of a bottle cap of a bacteria culture bottle transferred by the transfer mechanism 3, an injection mechanism 6 having a vessel 61 for holding a bacterial strain in liquid state, sucking the strain in the vessel 61 with a pump 62 and transporting to a nozzle 63, inserting the nozzle 63 attached to an air-driven lifting table 64 into the bacteria culture bottle and injecting the bacterial strain, and an electric control set having sensors at positions corresponding to the nozzles 63, and automatically controlling the transportation of the bacteria culture bottle, the opening and closing of the bottle cap and the injection of the bacterial strain by controlling the transfer mechanism 3, the cap opening and closing mechanism and the injection mechanism 6. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

本発明は真菌の接種システムに係り、特に液体状態で接種を自動的に実施できる液体状態接種用自動化量産システムに関するものである。   The present invention relates to a fungal inoculation system, and more particularly to an automated mass production system for liquid state inoculation that can automatically perform inoculation in a liquid state.

目下、生物農薬において、真菌がよく使用されるものの一つであり、真菌は百種類の害虫を退治できるが、真菌を生産する方法は固体状態接種と液体状態接種とがよく採用され、固体状態接種技術は自動化生産設備が既に開発されたが、液体状態接種技術は実験室での人工接種の段階に止まっている。しかしながら、液体状態接種は固体状態接種技術と比較すると、次のようなメリットがある。
（イ）液体状態の菌種の純度がより高く、液体状態の菌種で接種すれば、接種作業の効率は固体状態の菌種よりも４〜５倍に向上する。
（ロ）液体状態の菌種の芽生え速度は雑菌よりも速く、雑菌は成長できないので、雑菌汚染の問題を克服することができ、品質が向上する。
（ハ）液体状態の菌種の流動浸透能力がより良く、培養土の内部に滲入できるので、接種した後に多数の箇所で芽生えることができ、芽生え速度が向上する。 At present, fungi are one of the most commonly used bio-pesticides, and fungi can combat hundreds of pests, but the methods of producing fungi are often used in solid state and liquid state inoculation. The inoculation technology has already been developed for automated production facilities, but the liquid state inoculation technology is still at the stage of artificial inoculation in the laboratory. However, liquid state inoculation has the following merits compared to solid state inoculation technology.
(B) If the purity of the liquid species is higher and the seed is inoculated with the liquid species, the efficiency of the inoculation operation is improved by 4 to 5 times that of the solid species.
(B) The germination rate of the bacterial species in the liquid state is faster than the germs, and the germs cannot grow, so the problem of germ contamination can be overcome and the quality is improved.
(C) Since the fluid infiltration ability of the bacterial species in the liquid state is better and can penetrate into the culture soil, it can grow at many places after inoculation, and the seedling speed is improved.

液体状態接種技術としては、例えば特許文献１で開示されているキノコの液体状態菌種接種製造方法とその接種装置が提案された。これは、一定数量の培養土を充填した栽培瓶を収容し輸送する機構であり、液体状態の菌種接種位置で各栽培瓶に対応してノズルによる接種部が設けてあり、前記接種部は、チューブを介して液体状態菌種が貯蔵してある培養タンクと連通して前記液体状態菌種を培養土に散布するためのノズルを有し、前記ノズルには散布する液体状態菌種の数量を制御するためのエヤ駆動バルブおよびタイマーが設けてある。   As a liquid state inoculation technique, for example, a mushroom liquid state inoculum production method and an inoculation device disclosed in Patent Document 1 have been proposed. This is a mechanism for storing and transporting a cultivation bottle filled with a certain amount of culture soil, and an inoculation part by a nozzle is provided corresponding to each cultivation bottle at a liquid species inoculation position, And a nozzle for spraying the liquid state bacterial species to the culture soil in communication with a culture tank in which the liquid state bacterial species are stored via a tube, and the number of liquid state bacterial species to be dispersed in the nozzle An air drive valve and a timer are provided for controlling the motor.

台湾特許第４９０２８６号Taiwan Patent No. 490286

本発明の主な目的は、液体状態で菌種を自動的に接種できる液体状態接種用自動化量産システムを提供する。   The main object of the present invention is to provide an automated mass production system for liquid state inoculation that can automatically inoculate bacterial species in the liquid state.

上記目的を達成するため、本発明に係る液体状態接種用自動化量産システムは、チェンバーと、輸送機構と、キャップ開閉機構と、注射機構と、電気制御組とを備え、前記チェンバーは、開閉可能であり、その内部には収容空間を有し、前記収容空間と連通する入り口および出口を有し、吸気口が設けられ、前記吸気口ではファン用ろ過組が設けられ、前記ファン用ろ過組により前記チェンバーに流入した空気が無塵になり、前記無塵空気が入り口と出口とから排出され、無汚染状態にするために、前記チェンバー内には殺菌ランプが設けられ、前記輸送機構は、細菌培養瓶を輸送するために前記チェンバーの入り口と出口とを貫通し、前記キャップ開閉機構は、前記チェンバーの収容空間内に設置され、輸送機構により送入された細菌培養瓶に対応するレールが設けられ、細菌培養瓶が前記レールに沿って前進し、前記レールが細菌培養瓶の瓶ネックの底縁に対応して設置され、細菌培養瓶はレールにより上への移動を制限され、前記レールの末段には細菌培養瓶に対応する揺動台座が設けられ、揺動台座において細菌培養瓶の瓶キャップの底縁の両側に対応する箇所では止め板が設けられ、細菌培養瓶の上方に対応して揺動台座にはエヤシリンダーが設けられ、前記エヤシリンダーには瓶キャップに対応する押し板が設けられ、前記エヤシリンダーが作動するときには、押し板と二つの止め板とが細菌培養瓶の瓶キャップを挟み、シリンダーにより前記揺動台座が上下に揺動するときに瓶キャップの開閉作業が実施され、前記注射機構は、液体状態の菌種を収容するための容器を有し、ポンプにより前記容器内の液体状態の菌種を吸い上げてノズルへ輸送し、前記注射機構は別にエヤ駆動昇降台座を有し、前記ノズルは前記エヤ駆動昇降台座に取付けられ、細菌培養瓶の瓶キャップが開けられた後、エヤ駆動昇降台座が降下し前記ノズルを細菌培養瓶内に挿入して液体状態の菌種を注入した後、前記注射機構が元の位置に戻り、前記電気制御組は、前記注射機構のノズルに対応する箇所にセンサーが設けられ、細菌培養瓶の瓶口がノズルに位置合わせされた後に信号を発生して前記電気制御組が前記輸送機構を停止し、前記キャップ開閉機構により瓶キャップが開けられて、前記注射機構により液体状態の菌種が注入され、前記キャップ開閉機構により瓶キャップを閉鎖した後、前記輸送機構を起動させる、という順序で自動的に上記プロセスを制御する。
本発明に係る液体状態接種用自動化量産システムによれば、液体状態で菌種を自動的に接種することができる。 To achieve the above object, an automated mass production system for liquid state inoculation according to the present invention comprises a chamber, a transport mechanism, a cap opening / closing mechanism, an injection mechanism, and an electric control set, and the chamber is openable / closable. And having an accommodating space therein, having an inlet and an outlet communicating with the accommodating space, provided with an intake port, and provided with a fan filter set at the intake port, In order to make the air flowing into the chamber dust-free and the dust-free air is exhausted from the entrance and the exit to make it clean, a sterilization lamp is provided in the chamber. Bacteria culture that penetrates through the inlet and outlet of the chamber for transporting the bottle, and the cap opening and closing mechanism is installed in the storage space of the chamber and fed by the transport mechanism The bacterial culture bottle is advanced along the rail, the rail is installed corresponding to the bottom edge of the bottle neck of the bacterial culture bottle, and the bacterial culture bottle is moved upward by the rail. A swing pedestal corresponding to the bacterial culture bottle is provided at the end of the rail, and stopper plates are provided at locations corresponding to both sides of the bottom edge of the bottle cap of the bacterial culture bottle in the swing pedestal. An air cylinder is provided on the rocking pedestal corresponding to the upper side of the culture bottle, and the air cylinder is provided with a push plate corresponding to the bottle cap. When the air cylinder is operated, the push plate and two stop plates are provided. When the oscillating pedestal swings up and down by the cylinder, the bottle cap is opened and closed by the cylinder, and the injection mechanism is a container for storing the bacterial species in the liquid state. And sucking up the liquid species in the container by a pump and transporting them to the nozzle. The injection mechanism has a separate air-driven lift platform, and the nozzle is attached to the air-driven lift platform, After the bottle cap is opened, the air drive lift base is lowered and the nozzle is inserted into the bacterial culture bottle to inject the liquid species, and then the injection mechanism returns to the original position, and the electric control The set is provided with a sensor at a location corresponding to the nozzle of the injection mechanism, generates a signal after the bottle mouth of the bacterial culture bottle is aligned with the nozzle, and the electric control set stops the transport mechanism, The bottle cap is opened by the cap opening / closing mechanism, the bacterial species in the liquid state is injected by the injection mechanism, the bottle cap is closed by the cap opening / closing mechanism, and then the transport mechanism is activated. Automatically controls the above process.
According to the automated mass production system for liquid state inoculation according to the present invention, bacterial species can be automatically inoculated in a liquid state.

以下、添付図面を参照して本発明の好適な実施の形態を詳細に説明する。
まず、図１から図７を参照する。もちろん、上記図面に示す実施例の構造は、本発明を説明するためのものであり、発明請求範囲を限定するためのものではない。
本発明に係る液体状態接種用自動化量産システムは、チェンバー１と、輸送機構３と、キャップ開閉機構５と、注射機構６と、電気制御組とから構成されたものである。 DESCRIPTION OF EMBODIMENTS Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
First, FIG. 1 to FIG. 7 will be referred to. Of course, the structure of the embodiment shown in the drawings is for explaining the present invention, and not for limiting the scope of the invention.
The automated mass production system for liquid state inoculation according to the present invention comprises a chamber 1, a transport mechanism 3, a cap opening / closing mechanism 5, an injection mechanism 6, and an electric control set.

前記チェンバー１は、開閉可能であり、その内部には収容空間１１を有し、前記収容空間１１と連通する入り口１１１および出口１１２を有し、吸気口１１３が設けてあり、前記吸気口１１３ではファン用ろ過組２が設けてあり、前記ファン用ろ過組２は、二つのファン組２１と、一つの高効率微細粒子ろ過材２２とを有し、これにより、前記チェンバー１に流入した空気が無塵になり、前記無塵空気が入り口１１１と出口１１２とから排出され、無汚染状態にするために、前記チェンバー１内には殺菌ランプ１２が設けてある。
前記輸送機構３は、細菌培養瓶４を輸送するために前記チェンバー１の入り口１１１と出口１１２とを貫通する。 The chamber 1 is openable and closable. The chamber 1 has an accommodating space 11 therein, an inlet 111 and an outlet 112 communicating with the accommodating space 11, and an air inlet 113. The air inlet 113 A fan filtration set 2 is provided, and the fan filtration set 2 has two fan sets 21 and one high-efficiency fine particle filter 22 so that the air flowing into the chamber 1 is A sterilization lamp 12 is provided in the chamber 1 so that the dust-free air is discharged from the inlet 111 and the outlet 112 to make it dust-free.
The transport mechanism 3 passes through the inlet 111 and the outlet 112 of the chamber 1 to transport the bacterial culture bottle 4.

前記キャップ開閉機構５は、前記チェンバー１の収容空間１１内に設置され、輸送機構３により送入された細菌培養瓶４の瓶キャップ４１に対して開閉作業を実施し、輸送機構３により送入された細菌培養瓶４に対応するレール５１が設けられる。本実施例では、細菌培養瓶４がレール５１に沿って前進するために、四つのレール５１が互いに平行に並んでおり、前記レール５１が細菌培養瓶４の瓶ネック４２の底縁４２１に対応して設置され、細菌培養瓶４がレール５１に制限されて上へ移動できなくなっている。前記レール５１の末段には細菌培養瓶４に対応する揺動台座５２が設けられ、揺動台座５２において細菌培養瓶４の瓶キャップ４１の底縁の両側に対応する箇所では止め板５２１が設けられ、細菌培養瓶４の上方に対応して揺動台座５２にはエヤシリンダー５３が設けられ、前記エヤシリンダー５３には瓶キャップ４１に対応する押し板５３１が設けられる。前記エヤシリンダー５３が作動するときには、押し板５３１と二つの止め板５２１とが細菌培養瓶４の瓶キャップ４１を挟み、シリンダー５４により前記揺動台座５２が上下に揺動するときに瓶キャップの開閉作業が実施される。   The cap opening / closing mechanism 5 is installed in the accommodation space 11 of the chamber 1, performs an opening / closing operation on the bottle cap 41 of the bacterial culture bottle 4 fed by the transport mechanism 3, and is fed by the transport mechanism 3. A rail 51 corresponding to the bacterial culture bottle 4 is provided. In this embodiment, since the bacterial culture bottle 4 advances along the rail 51, the four rails 51 are arranged in parallel to each other, and the rail 51 corresponds to the bottom edge 421 of the bottle neck 42 of the bacterial culture bottle 4. The bacterial culture bottle 4 is restricted by the rail 51 and cannot move upward. A swinging pedestal 52 corresponding to the bacterial culture bottle 4 is provided at the end of the rail 51, and stopper plates 521 are provided at locations corresponding to both sides of the bottom edge of the bottle cap 41 of the bacterial culture bottle 4 in the swinging pedestal 52. An air cylinder 53 is provided on the rocking pedestal 52 corresponding to the upper side of the bacterial culture bottle 4, and a push plate 531 corresponding to the bottle cap 41 is provided on the air cylinder 53. When the air cylinder 53 operates, the push plate 531 and the two stop plates 521 sandwich the bottle cap 41 of the bacterial culture bottle 4, and when the rocking pedestal 52 swings up and down by the cylinder 54, Opening and closing work is performed.

前記注射機構６は、液体状態の菌種を収容するための二つの容器６１を有し、ポンプ６２により前記容器６１内の液体状態の菌種を吸い上げてノズル６３へ輸送する。前記注射機構６は別にエヤ駆動昇降台座６４を有し、前記ノズルおよびポンプ６２が前記エヤ駆動昇降台座６４に取付けられ、細菌培養瓶４の瓶キャップ４１が開けられた後、エヤ駆動昇降台座６４が降下し前記ノズル６３を細菌培養瓶４内に挿入して液体状態の菌種を注入した後、前記注射機構６は元の位置に戻る。   The injection mechanism 6 has two containers 61 for storing liquid bacterial species. The pump 62 sucks up the liquid bacterial species in the container 61 and transports them to the nozzle 63. The injection mechanism 6 has an air-driven lift base 64 separately. The nozzle and pump 62 are attached to the air-drive lift base 64, and after the bottle cap 41 of the bacterial culture bottle 4 is opened, the air-driven lift base 64 And the nozzle 63 is inserted into the bacterial culture bottle 4 to inject the bacterial species in a liquid state, and then the injection mechanism 6 returns to the original position.

前記注射機構６は更にコンプレッサー６５を含み、前記コンプレッサー６５の出力チューブ６５１には、フィルタ６５２と、圧力レギュレータ（図示せず）とが設けられ、前記フィルタ６５２はエヤ中にあるホコリおよび細菌を取り除くための空気ろ過フィルムであり、無塵無菌の空気は、前記容器６１内に注入され、且つポンプ６２の動力源として使用され、前記圧力レギュレータは、前記電気制御組により制御され、液体状態の菌種の注入量を調節するためのものである。   The injection mechanism 6 further includes a compressor 65, and an output tube 651 of the compressor 65 is provided with a filter 652 and a pressure regulator (not shown). The filter 652 removes dust and bacteria in the air. The air filter film is dust-free aseptic air is injected into the container 61 and used as a power source for the pump 62, and the pressure regulator is controlled by the electric control set, and is in a liquid state. It is for adjusting the injection amount of seeds.

前記電気制御組は、前記注射機構６のノズル６３に対応する箇所でセンサー７１が設けられ（電気制御組は電気回路であるので、全体を表示せず、センサーだけを表示し）、細菌培養瓶４の瓶口がノズル６３に位置合わせされた後に信号を発生して前記電気制御組が前記輸送機構３を停止して、前記キャップ開閉機構５により瓶キャップ４１が開けられて、前記注射機構６により液体状態の菌種が注入されて、前記キャップ開閉機構５により瓶キャップ４１が閉鎖された後、前記輸送機構３を起動させる、という順序で自動的に上記プロセスを制御する。   The electric control group is provided with a sensor 71 at a position corresponding to the nozzle 63 of the injection mechanism 6 (since the electric control group is an electric circuit, only the sensor is displayed without displaying the whole), and the bacterial culture bottle After the 4 bottle mouths are aligned with the nozzle 63, a signal is generated, the electric control group stops the transport mechanism 3, the bottle cap 41 is opened by the cap opening / closing mechanism 5, and the injection mechanism 6 Thus, the above process is automatically controlled in the order of injecting the liquid bacterial species and closing the bottle cap 41 by the cap opening / closing mechanism 5 and then starting the transport mechanism 3.

本発明の一実施例に係る液体状態接種用自動化量産システムは、作業する前に液体状態の菌種を調製して、液体状態の菌種を注射機構６の容器６１内に注入することが必要である。液体状態の菌種の準備作業が完成した後、前記輸送機構３により細菌培養瓶４をチェンバー１の入り口１１を経由して収容空間１１内に送入し、細菌培養瓶４が送入されたときには、図４に示すように、細菌培養瓶４はキャップ開閉機構５のレール５１に沿って前進する。   The automated mass production system for inoculating a liquid state according to an embodiment of the present invention needs to prepare the bacterial species in the liquid state before working and inject the bacterial species in the liquid state into the container 61 of the injection mechanism 6. It is. After the preparation of the bacterial species in the liquid state was completed, the bacterial culture bottle 4 was sent into the accommodation space 11 via the entrance 11 of the chamber 1 by the transport mechanism 3, and the bacterial culture bottle 4 was sent in. Sometimes, the bacterial culture bottle 4 moves forward along the rail 51 of the cap opening / closing mechanism 5 as shown in FIG.

前記チェンバー１の頂部の吸気口１１３ではファン用ろ過組２が設けられ、前記ファン用ろ過組２により無塵空気がチェンバー１の収容空間１１内に送入され、無塵空気が入り口１１１と出口１１２とを経由して排出される。これにより、チェンバー１の収容空間１１内に外部の空気が進入できないので、液体状態の菌種が汚染される可能性は降下する。   The air inlet 113 at the top of the chamber 1 is provided with a fan filtration set 2, and the fan filtration set 2 feeds dust-free air into the accommodating space 11 of the chamber 1, and dust-free air flows into the inlet 111 and the outlet. 112 and discharged. Thereby, since external air cannot enter into the storage space 11 of the chamber 1, the possibility that the bacterial species in the liquid state are contaminated is lowered.

電気制御組のセンサー７１は、細菌培養瓶４の瓶口がノズル６３に位置合わせされたと感知した後に、前記輸送機構３を停止する。このとき、細菌培養瓶４の瓶ネック４２の底縁４２１はレール５１に当接し、細菌培養瓶４の瓶キャップ４１の底縁はキャップ開閉機構５の止め板５２１に当接する。電気制御組はエヤシリンダー５３を作動させて、図５に示すように、押し板５３１と二つの止め板５２１とにより細菌培養瓶４の瓶キャップ４１を挟み、前記電気制御組はシリンダー５４を駆動し、前記シリンダー５４により揺動台座５２を上へ揺動し、これにより、図６に示すように、瓶キャップ４１が開けられ、細菌培養瓶４はレール５１に制限され元の位置に止まる。図７に示すように、前記電気制御組は注射機構６のエヤ駆動昇降台座６４を降下することにより、ノズル６３を細菌培養瓶４内に挿入して液体状態の菌種を注入する。前記電気制御組は圧力レギュレータを制御することにより液体状態の菌種の注入数量は制御可能である。   The sensor 71 of the electric control group stops the transport mechanism 3 after detecting that the bottle mouth of the bacterial culture bottle 4 is aligned with the nozzle 63. At this time, the bottom edge 421 of the bottle neck 42 of the bacteria culture bottle 4 contacts the rail 51, and the bottom edge of the bottle cap 41 of the bacteria culture bottle 4 contacts the stopper plate 521 of the cap opening / closing mechanism 5. The electric control group operates the air cylinder 53, and as shown in FIG. 5, the bottle cap 41 of the bacterial culture bottle 4 is sandwiched between the push plate 531 and the two stop plates 521, and the electric control group drives the cylinder 54. Then, the rocking pedestal 52 is swung upward by the cylinder 54, whereby the bottle cap 41 is opened as shown in FIG. 6, and the bacteria culture bottle 4 is restricted by the rail 51 and stops at the original position. As shown in FIG. 7, the electric control group lowers the air drive lift base 64 of the injection mechanism 6, thereby inserting the nozzle 63 into the bacterial culture bottle 4 and injecting the bacterial species in the liquid state. The electric control group can control the injection quantity of the bacterial species in the liquid state by controlling the pressure regulator.

液体状態の菌種の注入が完成した後、前記電気制御組は注射機構６にあるエヤ駆動昇降台座６４を元位置に戻して、キャップ開閉機構５により瓶キャップ４１を閉鎖して、前記輸送機構３により細菌培養瓶４を前進させると、自動化接種作業が完成する。このとき、次回の液体状態での接種作業を実施することができる。   After the injection of the bacterial species in the liquid state is completed, the electric control group returns the air drive lift base 64 in the injection mechanism 6 to the original position, closes the bottle cap 41 by the cap opening / closing mechanism 5, and the transport mechanism When the bacterial culture bottle 4 is moved forward by 3, the automated inoculation operation is completed. At this time, inoculation work in the next liquid state can be performed.

本発明の一実施例に係る液体状態接種用自動化量産システムの概略図である。1 is a schematic view of an automated mass production system for liquid state inoculation according to an embodiment of the present invention. 本発明の一実施例に係る液体状態接種用自動化量産システムの配置を示す概略図である。It is the schematic which shows arrangement | positioning of the automatic mass production system for liquid state inoculations concerning one Example of this invention. 本発明の一実施例に係るキャップ開閉機構の構造を示す概略図である。It is the schematic which shows the structure of the cap opening / closing mechanism which concerns on one Example of this invention. 本発明の一実施例に係るキャップ開閉機構の構造を示す概略図である。It is the schematic which shows the structure of the cap opening / closing mechanism which concerns on one Example of this invention. 本発明の一実施例に係る液体状態接種用自動化量産システムにより自動化接種を実施する動作を示す概略図である。It is the schematic which shows the operation | movement which implements automatic inoculation by the automatic mass production system for liquid state inoculations concerning one Example of this invention. 本発明の一実施例に係る液体状態接種用自動化量産システムにより自動化接種を実施する動作を示す概略図である。It is the schematic which shows the operation | movement which implements automatic inoculation by the automatic mass production system for liquid state inoculations concerning one Example of this invention. 本発明の一実施例に係る液体状態接種用自動化量産システムにより自動化接種を実施する動作を示す概略図である。It is the schematic which shows the operation | movement which implements automatic inoculation by the automatic mass production system for liquid state inoculations concerning one Example of this invention.

符号の説明Explanation of symbols

１ チェンバー、１１ 収容空間、１１１ 入り口、１１２ 出口、１１３ 吸気口、１２ 殺菌ランプ、２ ファン用ろ過組、２１ ファン組、２２ 高効率微細粒子ろ過材、３ 輸送機構、４ 細菌培養瓶、４１ 瓶キャップ、４２ 瓶ネック、４２１ 底縁、５ キャップ開閉機構、５１ レール、５２ 揺動台座、５２１ 止め板、５３ エヤシリンダー、５３１ 押し板、５４ シリンダー、６ 注射機構、６１ 容器、６２ ポンプ、６３ ノズル、６４ エヤ駆動昇降台座、６５ コンプレッサー、６５１ 出力チューブ、６５２ フィルタ、７１ センサー、
DESCRIPTION OF SYMBOLS 1 Chamber, 11 Accommodating space, 111 Inlet, 112 outlet, 113 Inlet, 12 Sterilization lamp, 2 Fan filtration group, 21 Fan group, 22 High-efficiency fine particle filter material, 3 Transport mechanism, 4 Bacterial culture bottle, 41 bottle Cap, 42 Bottle neck, 421 Bottom edge, 5 Cap opening / closing mechanism, 51 Rail, 52 Swing base, 521 Stop plate, 53 Air cylinder, 531 Press plate, 54 Cylinder, 6 Injection mechanism, 61 Container, 62 Pump, 63 Nozzle 64 air drive lift base, 65 compressor, 651 output tube, 652 filter, 71 sensor,

Claims (5)

チェンバーと、輸送機構と、キャップ開閉機構と、注射機構と、電気制御組とを備え、
前記チェンバーは、開閉可能であり、その内部には収容空間を有し、前記収容空間と連通する入り口および出口を有し、吸気口が設けられ、前記吸気口ではファン用ろ過組が設けられ、前記ファン用ろ過組により前記チェンバーに流入した空気が無塵になり、前記無塵空気が入り口と出口とから排出され、無汚染状態にするために、前記チェンバー内には殺菌ランプが設けられ、
前記輸送機構は、細菌培養瓶を輸送するために前記チェンバーの入り口と出口とを貫通し、
前記キャップ開閉機構は、前記チェンバーの収容空間内に設置され、輸送機構により送入された細菌培養瓶に対応するレールが設けられ、細菌培養瓶が前記レールに沿って前進し、前記レールが細菌培養瓶の瓶ネックの底縁に対応して設置され、細菌培養瓶はレールにより上への移動を制限され、前記レールの末段には細菌培養瓶に対応する揺動台座が設けられ、揺動台座において細菌培養瓶の瓶キャップの底縁の両側に対応する箇所では止め板が設けられ、細菌培養瓶の上方に対応して揺動台座にはエヤシリンダーが設けられ、前記エヤシリンダーには瓶キャップに対応する押し板が設けられ、前記エヤシリンダーが作動するときには、押し板と二つの止め板とが細菌培養瓶の瓶キャップを挟み、シリンダーにより前記揺動台座が上下に揺動するときに瓶キャップの開閉作業が実施され、
前記注射機構は、液体状態の菌種を収容するための容器を有し、ポンプにより前記容器内の液体状態の菌種を吸い上げてノズルへ輸送し、前記注射機構は別にエヤ駆動昇降台座を有し、前記ノズルは前記エヤ駆動昇降台座に取付けられ、細菌培養瓶の瓶キャップが開けられた後、エヤ駆動昇降台座が降下し前記ノズルを細菌培養瓶内に挿入して液体状態の菌種を注入した後、前記注射機構が元の位置に戻り、
前記電気制御組は、前記注射機構のノズルに対応する箇所にセンサーが設けられ、細菌培養瓶の瓶口がノズルに位置合わせされた後に信号を発生して前記電気制御組が前記輸送機構を停止し、前記キャップ開閉機構により瓶キャップが開けられて、前記注射機構により液体状態の菌種が注入されて、前記キャップ開閉機構により瓶キャップを閉鎖した後、前記輸送機構を起動させる、という順序で自動的に上記プロセスを制御することを特徴とする液体状態接種用自動化量産システム。 A chamber, a transport mechanism, a cap opening / closing mechanism, an injection mechanism, and an electric control set;
The chamber is openable and closable, has an accommodating space therein, has an inlet and an outlet communicating with the accommodating space, is provided with an air inlet, and is provided with a fan filtration set at the air inlet. In order for the air flowing into the chamber by the fan filtration set to be dust-free, the dust-free air is discharged from the entrance and the exit, and in order to make it a pollution-free state, a sterilization lamp is provided in the chamber,
The transport mechanism penetrates the entrance and exit of the chamber to transport the bacterial culture bottle;
The cap opening and closing mechanism is installed in a storage space of the chamber, provided with a rail corresponding to the bacterial culture bottle fed by the transport mechanism, the bacterial culture bottle advances along the rail, and the rail is a bacteria The bacterial culture bottles are installed corresponding to the bottom edge of the bottle neck of the culture bottle, the upward movement of the bacterial culture bottle is restricted by the rail, and a swinging pedestal corresponding to the bacterial culture bottle is provided at the end of the rail. Stop plates are provided at locations corresponding to both sides of the bottom edge of the bottle cap of the bacterial culture bottle on the moving base, and an air cylinder is provided on the rocking base corresponding to the upper side of the bacterial culture bottle. When a push plate corresponding to the bottle cap is provided and the air cylinder is operated, the push plate and the two stopper plates sandwich the bottle cap of the bacterial culture bottle, and the rocking base is swung up and down by the cylinder. Closing operations of the bottle cap is performed when,
The injection mechanism has a container for storing the bacterial species in a liquid state, and sucks the liquid bacterial species in the container by a pump and transports it to the nozzle. The injection mechanism has an air-driven lift base. The nozzle is attached to the air-driven elevating pedestal, and after the bottle cap of the bacterial culture bottle is opened, the air-driven elevating pedestal descends and the nozzle is inserted into the bacterial culture bottle to remove the liquid species. After injection, the injection mechanism returns to its original position,
The electric control group is provided with a sensor at a position corresponding to the nozzle of the injection mechanism, generates a signal after the bottle mouth of the bacterial culture bottle is aligned with the nozzle, and the electric control group stops the transport mechanism Then, the bottle cap is opened by the cap opening / closing mechanism, the bacterial species in a liquid state is injected by the injection mechanism, the bottle cap is closed by the cap opening / closing mechanism, and then the transport mechanism is activated. An automated mass production system for liquid state inoculation characterized by automatically controlling the above process. 前記ファン用ろ過組は、二つのファン組と、高効率微細粒子ろ過材とを有することを特徴とする請求項１に記載の液体状態接種用自動化量産システム。   The automated mass production system for liquid state inoculation according to claim 1, wherein the fan filtration set includes two fan sets and a high-efficiency fine particle filter medium. 前記注射機構は更にコンプレッサーを含み、前記コンプレッサーの出力チューブには、フィルタと、圧力レギュレータとが設けてあり、前記フィルタはエヤ中にあるホコリおよび細菌を取り除くためのものであり、無塵無菌の空気は、前記容器内に注入され、且つポンプの動力源として使用されることを特徴とする請求項１に記載の液体状態接種用自動化量産システム。   The injection mechanism further includes a compressor, and an output tube of the compressor is provided with a filter and a pressure regulator. The filter is for removing dust and bacteria in the air, and is dust-free and sterile. The automated mass production system for liquid state inoculation according to claim 1, wherein air is injected into the container and used as a power source of a pump. 前記注射機構のフィルタは空気ろ過フィルムであることを特徴とする請求項１に記載の液体状態接種用自動化量産システム。   The automated mass production system for liquid state inoculation according to claim 1, wherein the filter of the injection mechanism is an air filtration film. 前記圧力レギュレータは、前記電気制御組により制御され、液体状態の菌種の注入量を調節するためのものであることを特徴とする請求項３に記載の液体状態接種用自動化量産システム。   The automated mass production system for liquid state inoculation according to claim 3, wherein the pressure regulator is controlled by the electric control group and is for adjusting the injection amount of the bacterial species in the liquid state.
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JP2015077680A (en) * 2013-10-18 2015-04-23 ポール ライフ サイエンシズ ベルジウム ビーヴイビーエーＰａｌｌ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｂｅｌｇｉｕｍ Ｂｖｂａ Disposable production line for filling and finishing product
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CN105359824A (en) * 2015-10-28 2016-03-02 江苏久禾生物科技发展有限公司 Method for setting industrial-cultivation mushroom fruiting rooms of pleurotus eryngii
CN107873400A (en) * 2017-11-13 2018-04-06 贵州绿源芊翠农业开发有限公司 A kind of black fungus strain injection device
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