CN2052771U

CN2052771U - Simple liquid strain production device

Info

Publication number: CN2052771U
Application number: CN 89203999
Authority: CN
Inventors: 侯建华
Original assignee: Individual
Current assignee: Individual
Priority date: 1989-04-03
Filing date: 1989-04-03
Publication date: 1990-02-14

Abstract

The utility model belongs to a device for producing liquid edible bacterial strain. At present, the equipment for cultivating liquid bacterial strain is complex and high in fabricating cost, so the technique for cultivating liquid bacterial strain can not be easily controlled. The utility model can provide a simple liquid bacterial strain producing device which is easy in production and low in cost for the society. The utility model is characterized in that the device is provided with an ultra-clean filter, an inoculator and a multilevel air filter; the whole of the simple liquid bacterial strain production device adopts a closed pipeline to conduct production. Cultivating liquid used by the utility model doesn't need to conduct autoclaving process and the cultivating liquid can conduct closed type continuous culture, so the utility model is a device which has the advantages of less investment, obvious effect and strong practicability.

Description

Simple liquid strain production device

The invention belongs to a kind of device of producing the liquid edible bacterial classification.

Current, China's edible mushrooms career development is rapid, is producing yet a lot of edible mushrooms field and specialist are continuing to use old-fashioned solid spawn method always.Produce the solid spawn complex operation, labour intensity is big, and the production cycle is long, and bacterium makes inconsistent, and aging easily, and cost is also quite high.In recent years, all industrial fermentation technology is applied to aspect the edible fungus culturing both at home and abroad, so liquid spawn occurred in research.Liquid spawn is compared with solid spawn, and it is consistent, with short production cycle to have a bacterium order, and mechanize is convenient in inoculation, and output such as can increase substantially at advantage.But, cultivate the used equipment complexity of liquid spawn at present, technology is difficult to be grasped, and invests very big (generally needing several ten thousand yuan to hundreds of thousands of unit), is difficult for popularizing.

The objective of the invention is provides a kind of liquid spawn production equipment simple, that be convenient to grasp of making to society.

Fig. 1 is a structural representation of the present invention;

Fig. 2 is the structural representation of multistage air cleaner ［ 38 ］;

Fig. 3 is the structural representation of inoculator ［ 34 ］.

The present invention is as shown in Figure 1: ［ 1 ］, ［ 13 ］, ［ 30 ］, ［ 36 ］ are container; ［ 2 ］, ［ 14 ］, ［ 21 ］, ［ 22 ］, ［ 27 ］, ［ 32 ］ are plug; ［ 3 ］, ［ 19 ］, ［ 24 ］, for the exhaust bulb; ［ 4 ］, ［ 16 ］, ［ 23 ］, ［ 28 ］, ［ 29 ］ are air inlet pipe; ［ 17 ］, ［ 35 ］ are woven hose; ［ 20 ］ be ultra-clean filter (in-built screen pack, filter paper, active carbon); ［ 6 ］, ［ 8 ］, ［ 9 ］, ［ 12 ］, ［ 15 ］, ［ 18 ］, ［ 25 ］, ［ 33 ］, ［ 37 ］ are tongs; ［ 5 ］, ［ 7 ］, ［ 10 ］, ［ 11 ］, ［ 26 ］ are three-way pipe; ［ 34 ］ be inoculator; ［ 38 ］ be multistage air cleaner; ［ 39 ］ be air pump. Fig. 1 illustrates that also this device all adopts closed conduit to produce.

Fig. 2 is the structural representation of multistage air cleaner ［ 38 ］: air pump ［ 39 ］ produces the stingy piezometric air that contracts, the in-line filter ［ 42 ］ that gas filters bulb ［ 40 ］, Y-tube ［ 41 ］ and gac is housed through giving of interior dress degreasing cotton, enter the container ［ 44 ］ from inlet pipe ［ 43 ］, the air outlet through passing plug ［ 45 ］［ 46 ］, Y-tube ［ 47 ］ divide two the road to insert original device again.

Fig. 3 is the structural representation of inoculator ［ 34 ］: inlet pipe ［ 31 ］, tongs ［ 33 ］ and exhaust bulb ［ 48 ］ are housed on the container ［ 49 ］, container is equipped with cultured liquid spawn in ［ 49 ］, is sucked by syringe ［ 54 ］ by suction pipe ［ 51 ］, valve ［ 52 ］, the Y-tube ［ 53 ］ that passes in plug ［ 50 ］; Bacterium hydraulic pressure in the syringe ［ 54 ］ is gone out,, inject manifold ［ 56 ］, by tubing ［ 57 ］, injection needles ［ 59 ］ liquid bacterium liquid is expelled in culture bottle or the bag again and (also can directly goes up inoculation) toward bed by valve ［ 55 ］; ［ 58 ］ be adjustable needle tubing support.

Principle of work of the present invention and operating process are as follows: the nutrient solution for preparing is packed in the container ［ 1 ］, container ［ 1 ］ mouth is installed the plug ［ 2 ］ that has inlet pipe ［ 4 ］ and exhaust bulb ［ 3 ］ (unskimmed high-quality cotton is housed in the bulb), bandages then and is placed on 1.1 kilograms/cm ²Down sterilization 30 minutes of high pressure, cooling back aseptic technique inserts that slant tube is female plants, and simultaneously inlet pipe ［ 4 ］ and Y-tube ［ 5 ］ is connected.Leave standstill and cultivated 1～2 day, room temp remains between 25～27 ℃.Begin aerated culture behind the mycelium germination, at this moment close tongs ［ 6 ］, ［ 8 ］, ［ 12 ］, ［ 37 ］, booster air pump ［ 39 ］; When the mycelium pellet in the container ［ 1 ］ accounts for liquid volume when over half, the one-level kind is cultivated and is promptly come to an end.At this moment tongs ［ 9 ］ is also closed, with interim sebific duct (bacterium of having gone out), the front end and the inlet pipe ［ 16 ］ of tongs ［ 12 ］ are coupled together, then tongs ［ 12 ］, ［ 15 ］, ［ 18 ］ are opened, booster air pump ［ 39 ］ is pressed into the concentrated nutrient solution in the container ［ 13 ］ in the container ［ 36 ］.Close tongs ［ 18 ］, to inject container ［ 36 ］ according to quantity through filtering tap water of ultra-clean strainer ［ 20 ］ or well water, after enrichment medium mixes, open tongs ［ 25 ］, mixed solution is flowed in the container ［ 30 ］, after enrichment medium mixes, open tongs ［ 25 ］, mixed solution is flowed in the container ［ 30 ］, close tongs ［ 25 ］ then.Tongs ［ 15 ］ is closed, one end of interim sebific duct is removed from inlet pipe ［ 16 ］, be positioned on the exhaust bulb ［ 3 ］, open tongs ［ 6 ］ booster air pump, cultured liquid spawn in the container ［ 1 ］ is pressed in the container ［ 30 ］, and inoculum size is 10%.Remove interim sebific duct then, close tongs ［ 6 ］, ［ 9 ］, ［ 12 ］, ［ 25 ］, open tongs ［ 8 ］, ［ 37 ］, booster air pump, sterile air after multistage filter ［ 38 ］ filters divides two-way to enter in the container ［ 30 ］.Inlet pipe ［ 28 ］ is in deep liquid, and inlet pipe ［ 29 ］ is on the liquid upper strata, about two day time liquid spawn can grow.At this moment, close air pump ［ 39 ］ and tongs ［ 8 ］, ［ 37 ］, open tongs ［ 33 ］, it is standby that liquid spawn is flowed in the inoculator ［ 34 ］.Cultured continuously can make each surplus bacterial classification of 10% that has in the container ［ 30 ］ if desired, fresh medium is injected to get final product then.

The present invention makes simply, and less investment is practical.Particularly nutrient solution does not need whole autoclavings, just can carry out closed cultured continuously, and a kind of really effect is splendid, is suitable for the device of popularization.

Fig. 1 of the present invention, Fig. 2, container, exhaust bulb, tubing, Y-tube shown in Figure 3, give and filter bulb, in-line filter, suction pipe, valve, syringe, manifold and be all glasswork; Tongs is metal or plastics; Injection needles, adjustable needle tubing support are metal products; Air pump is a standardized component; Plug is plastics or woodwork.After above-mentioned article were got all the ready, by the figure assembling, every pipe connection was all used medical sebific duct.

Claims

1, a kind of device of producing the liquid edible bacterial classification, it comprises container, tongs, Y-tube, air pump etc., it is characterized in that this device has ultra-clean strainer [20] (interior dress filtering net, filter paper, gac), inoculator [34] and multistage air cleaner [38].

2, a kind of device of producing the liquid edible bacterial classification according to claim 1 is characterized in that multistage air cleaner ［ 38 ］ filters bulb ［ 40 ］, Y-tube ［ 41 ］ by giving, contact strainer ［ 42 ］, inlet pipe ［ 43 ］, container ［ 44 ］, plug ［ 45 ］, air outlet ［ 46 ］ and Y-tube ［ 47 ］ are formed.

3, a kind of device of producing the liquid edible bacterial classification according to claim 1 is characterized in that inoculator ［ 34 ］ is by container ［ 49 ］, plug ［ 50 ］, suction pipe ［ 51 ］, valve ［ 52 ］, Y-tube ［ 53 ］, syringe ［ 54 ］, valve ［ 55 ］, manifold ［ 56 ］, tubing ［ 57 ］, adjustable needle tubing support ［ 58 ］ with firmly penetrate syringe needle ［ 59 ］ and form.