CN101940129A - Method and device for preparing pleurotus eryngii liquid strain - Google Patents
Method and device for preparing pleurotus eryngii liquid strain Download PDFInfo
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- CN101940129A CN101940129A CN2010102766549A CN201010276654A CN101940129A CN 101940129 A CN101940129 A CN 101940129A CN 2010102766549 A CN2010102766549 A CN 2010102766549A CN 201010276654 A CN201010276654 A CN 201010276654A CN 101940129 A CN101940129 A CN 101940129A
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Abstract
The invention discloses a method and a device for preparing a pleurotus eryngii liquid strain. The method for preparing the liquid strain comprises the following step of: 1, preparing liquid strain culture medium from the following materials in percentage by weight: 15 to 25 percent of potato, 5 to 15 percent of wheat bran, 1 to 5 percent of brown sugar, 0.5 to 3 percent of glucose, 0.2 to 1.5 percent of peptone, 0.2 to 1.5 percent of potassium dihydrogen phosphate, 0.05 to 0.3 percent of magnesium sulfate, 0.05 to 0.3 percent of vitamin B, 0.01 to 0.05 percent of plant oil and the balance of water, wherein the pH value is 5.0 to 8.0. An air purification device provided by the invention is formed by arranging an oil-free air compressor at an outdoor front air supply end; a filter membrane with small aperture is selected; double filtration is performed on air by air intake and air outtake, and the air then is fed in an air storage tank and then fed in an indoor branch cylinder through a pipeline, passes through a reducing valve, is filtered and fed in each fermentation tank through a filter of the fermentation tank; in this way, the outdoor air is filtered for several time to become clean and aseptic fresh air which is fed in the fermentation tank; and thus the clean air required for fermentation culture of the liquid strain in the tank can be effectively ensured, and the preparation of the liquid strain is facilitated.
Description
Technical field:
The invention belongs to the fungus growing technique field, refer more particularly to preparation method and equipment that edible fungi liquid strain is produced in batch production.
Background technology:
The liquid spawn manufacturing technology is novel production method in the Edible Fungi.It is the application of biofermentation technique in agricultural production.Along with the raising of the scale of the development of Edible Fungi technology and production, batch production, up-to-dateness, the liquid spawn manufacturing technology will more and more have application value widely.
Liquid spawn is made according to edible mushroom mycelium the nutritional condition requirement, the obtaining liq medium, the production technology of utilizing the strain cultivation jar and matching, artificial button are provided with in the liquid culture jar hypha of edible fungus required environmental parameter of growing, the process of cultivating mycelium pellet in a large number.Liquid spawn is compared with solid spawn, and it is with short production cycle to have a bacterial classification, the cell age neat and consistent; mycelia is energetic, and inoculation is convenient, advantages such as unsuitable bacteria infection; be connected in the solid culture medium, good fluidity, it is many to sprout point; mycelial growth is fast and even; fruiting is neat, output height, quality better; be more suitable for scale, batch production Edible Fungi, have good development prospect.
The production of existing Xingbao mushroom liquid bacterium is that the female kind → one-level of primary inclined plane is shaken the method that bottle kind → secondary shakes bottle kind → culture tank (liquid spawn).This method is long because of growth cycle, reasons such as investment is big, the pollution of technology difficulty or ease, cost height, is difficult to large-scale popularization and uses.In addition, what liquid spawn produced most critical is to cultivate in the liquid clean, sufficient oxygen meltage will be arranged, because, oxygen abundance in the culture fluid, mycelium could be grown by normal growth, the meltage of oxygen in culture fluid also has direct relation with the quality of air cleaning unit and purification, and be simultaneously also relevant with the contact area and the osmotic pressure of liquid.
Summary of the invention:
Purpose of the present invention is exactly the method and apparatus that a kind of new production Xingbao mushroom liquid spawn is provided in order to solve the problem that technology exists in the existing Xingbao mushroom liquid spawn preparation.
The objective of the invention is to be achieved through the following technical solutions.
Preparation method of a kind of Xingbao mushroom liquid spawn and device is characterized in that:
The preparation method of liquid spawn is:
1, the proportioning of liquid spawn culture medium: by weight percentage, potato 15-25, wheat bran 5-15, brown sugar 1-5, glucose 0.5-3, peptone 0.2-1.5, potassium dihydrogen phosphate 0.2-1.5, magnesium sulfate 0.05-0.3, Cobastab 0.05-0.3, vegetable oil 0.01-0.05, surplus is a water, pH value 5.0-8.0.
2, the preparation of feed liquid: in the following order
1. make murphy juice and wheat bran juice: fresh potato digs the bud peeling and cleans, and is cut into thick sheet in the 4cm left and right sides or bar, adds 5 times of poach and use 6-8 layer filtered through gauze when shortcake does not rot, and it is standby to get juice.The wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, and it is standby to cross leaching juice.
2. composite soil fermented bean drink and wheat bran juice.
3. will add in the mixed juice behind potassium dihydrogen phosphate, the molten water of magnesium sulfate.
4. will add in the mixed juice behind brown sugar, glucose, the molten water of peptone again.
5. Cobastab being pulverized the back adds in the mixed juice again.
6. be that 10% hydrochloric acid or sodium hydroxide are adjusted pH value to the scope of 5.0-8.0 with concentration.
7. sterilization: medium is packed in the culture tank, when temperature reaches 120-130 ℃, kept 30-35 minute.
8. cooling: after the sterilization, feed the cold water cooling immediately, when the jar internal pressure is reduced to 1kg/cm
2The time, by the air nozzle of tank base medium is inflated stirring, the input filtrated air is kept tank pressure 0.2-0.3kg/cm
2
3, inoculated and cultured: the special mother kind is put in the sterile water of the bacterium of having gone out; make bacteria suspension; when the temperature for the treatment of culture tank is reduced to 23-30 ℃; the inoculation of unlatching cover; and with flame circle protection inoculation mouthful; by the bacteria suspension that flame circle input makes, cover the inoculation mouth immediately, recover tank pressure to 0.2-0.3kg/cm
2, 24-26 ℃ of control cultivation temperature feeds filtrated air and cultivated 2-4 days, can form a large amount of bacterium balls, becomes liquid spawn.Through after the assay was approved, liquid spawn can be injected in the planting almond abalone mushroom bag.
The existing air cleaning of sending into fermentation tank is finished by an air feed pump, a filter, and filtered air is clean inadequately, often causes the assorted bacterium random thoughts of fermentation pot liquid bacterium to dye, and causes liquid spawn to make failure.Flow process is: outdoor air → air feed pump (filtration) → filter → fermentation tank
Air cleaning unit provided by the invention is at outdoor air feed tip oil-free air compressor to be installed, and the little filter membrane in selection aperture, the air process air inlet and the double filtration of giving vent to anger, enter in the air accumulator, again by in the sub-cylinder in the pipeline inlet chamber, by pressure-reducing valve and filtration, the filter through fermentation tank enters in each fermentation tank more again.Outdoor air becomes the fresh air of cleaning sterile through above multiple times of filtration, enters fermentation tank, guarantees the required pure air of liquid spawn fermented and cultured in jar effectively, helps the making of liquid spawn.
Description of drawings:
Accompanying drawing is an air cleaning unit flow chart of the present invention.
Embodiment:
The invention will be further described below in conjunction with embodiment and accompanying drawing.
Preparation method of a kind of Xingbao mushroom liquid spawn and device is characterized in that:
The preparation method of liquid spawn is:
1, the proportioning of liquid spawn culture medium: by weight percentage, potato 20, wheat bran 15, brown sugar 3, glucose 1, peptone 01, potassium dihydrogen phosphate 0.5, magnesium sulfate 0.05, Cobastab 0.05, vegetable oil 0.01, surplus is a water, pH value 7.5.
2, the preparation of feed liquid: in the following order
1. make murphy juice and wheat bran juice: fresh potato digs the bud peeling and cleans, and is cut into thick sheet in the 4cm left and right sides or bar, adds 5 times of poach and use 6-8 layer filtered through gauze when shortcake does not rot, and it is standby to get juice.The wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, and it is standby to cross leaching juice.
2. composite soil fermented bean drink and wheat bran juice.
3. will add in the mixed juice behind potassium dihydrogen phosphate, the molten water of magnesium sulfate.
4. will add in the mixed juice behind brown sugar, glucose, the molten water of peptone again.
5. Cobastab being pulverized the back adds in the mixed juice again.
6. be that 10% hydrochloric acid or sodium hydroxide are adjusted pH value to 7.5 with concentration.
7. sterilization: medium is packed in the culture tank, when temperature reaches 121 ℃, kept 30 minutes.
8. cooling: after the sterilization, feed the cold water cooling immediately, when the jar internal pressure is reduced to 1kg/cm
2The time, by the air nozzle of tank base medium is inflated stirring, the input filtrated air is kept tank pressure 0.2kg/cm
2
3, inoculated and cultured: the special mother kind is put in the sterile water of the bacterium of having gone out, made bacteria suspension, when the temperature for the treatment of culture tank is reduced to 25 ℃; unlatching cover inoculation, and with flame circle protection inoculation mouthful, the bacteria suspension that makes by flame circle input; cover the inoculation mouth immediately, recover tank pressure to 0.2kg/cm
2, 25 ℃ of control cultivation temperature feed filtrated air and cultivated 3 days, can form a large amount of bacterium balls, become liquid spawn.Through after the assay was approved, liquid spawn can be injected in the planting almond abalone mushroom bag.
Air cleaning unit provided by the invention is at outdoor air feed tip oil-free air compressor to be installed, and the little filter membrane in selection aperture, the air process air inlet and the double filtration of giving vent to anger, enter in the air accumulator, again by in the sub-cylinder in the pipeline inlet chamber, by pressure-reducing valve and filtration, the filter through fermentation tank enters in each fermentation tank more again.Outdoor air becomes the fresh air of cleaning sterile through above multiple times of filtration, enters fermentation tank, guarantees the required pure air of liquid spawn fermented and cultured in jar effectively, helps the making of liquid spawn.
Claims (2)
1. preparation method of an Xingbao mushroom liquid spawn and device, it is characterized in that: the preparation method of liquid spawn is:
(1) proportioning of liquid spawn culture medium: by weight percentage, potato 15-25, wheat bran 5-15, brown sugar 1-5, glucose 0.5-3, peptone 0.2-1.5, potassium dihydrogen phosphate 0.2-1.5, magnesium sulfate 0.05-0.3, Cobastab 0.05-0.3, vegetable oil 0.01-0.05, surplus is a water, pH value 5.0-8.0;
(2) preparation of feed liquid: in the following order
1. make murphy juice and wheat bran juice: fresh potato digs the bud peeling and cleans, be cut into thick sheet in the 4cm left and right sides or bar, add 5 times of poach and use 6-8 layer filtered through gauze when shortcake does not rot, it is standby to get juice, the wheat bran that fresh nothing is gone mouldy adds 10 times of water boils to 20 minute, and it is standby to cross leaching juice;
2. composite soil fermented bean drink and wheat bran juice;
3. will add behind potassium dihydrogen phosphate, the molten water of magnesium sulfate in the mixed juice;
4. will add behind brown sugar, glucose, the molten water of peptone in the mixed juice again;
5. Cobastab being pulverized the back adds in the mixed juice again;
6. be that 10% hydrochloric acid or sodium hydroxide are adjusted pH value to the scope of 5.0-8.0 with concentration;
7. sterilization: medium is packed in the culture tank, when temperature reaches 120-130 ℃, kept 30-35 minute;
8. cooling: after the sterilization, feed the cold water cooling immediately, when the jar internal pressure is reduced to 1kg/cm
2The time, by the air nozzle of tank base medium is inflated stirring, the input filtrated air is kept tank pressure 0.2-0.3kg/cm
2
(3) inoculated and cultured: the special mother kind is put in the sterile water of the bacterium of having gone out; make bacteria suspension; when the temperature for the treatment of culture tank is reduced to 23-30 ℃; the inoculation of unlatching cover; and with flame circle protection inoculation mouthful; by the bacteria suspension that flame circle input makes, cover the inoculation mouth immediately, recover tank pressure to 0.2-0.3kg/cm
2, 24-26 ℃ of control cultivation temperature feeds filtrated air and cultivated 2-4 days, can form a large amount of bacterium balls, becomes liquid spawn, through after the assay was approved, liquid spawn can be injected in the planting almond abalone mushroom bag.
2. preparation method of a kind of Xingbao mushroom liquid spawn according to claim 1 and device, it is characterized in that: air cleaning unit provided by the invention is at outdoor air feed tip oil-free air compressor to be installed, and the little filter membrane in selection aperture, the air process air inlet and the double filtration of giving vent to anger, enter in the air accumulator, again by in the sub-cylinder in the pipeline inlet chamber, again by pressure-reducing valve and filtration, filter through fermentation tank enters in each fermentation tank again, outdoor air is through above multiple times of filtration, become the fresh air of cleaning sterile, enter fermentation tank, guarantee the required pure air of liquid spawn fermented and cultured in jar effectively, help the making of liquid spawn.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391034A (en) * | 2011-03-24 | 2012-03-28 | 上海雪榕生物科技股份有限公司 | Formula and preparation method of Pleurotus eryngii liquid strain culture medium |
| CN102845220A (en) * | 2012-09-10 | 2013-01-02 | 阜阳市德益农业科技有限公司 | Three-stage filtering ventilation device for liquid strain fermentation tank |
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103340094A (en) * | 2013-06-26 | 2013-10-09 | 襄汾县侯临农业科技有限公司 | Pleurotus eryngii liquid culturing method |
| CN103416216A (en) * | 2012-05-23 | 2013-12-04 | 大连良农农业产业发展有限公司 | Formula for cultivating pleurotus eryngii liquid strain with corn stalks as base materials and preparation method thereof |
| CN103922842A (en) * | 2014-04-17 | 2014-07-16 | 四川省砚山中药饮片有限公司 | Method for preparing pleurotus eryngii culture medium |
| CN104311348A (en) * | 2014-10-22 | 2015-01-28 | 常熟理工学院 | Improved liquid fermentation culture medium of pleurotus eryngii and method for culturing liquid strain of pleurotus eryngii by utilizing improved liquid fermentation culture medium |
| CN104429583A (en) * | 2013-09-13 | 2015-03-25 | 孙君莲 | Edible fungus liquid strain culturing method |
| CN104604527A (en) * | 2015-02-11 | 2015-05-13 | 北京华绿生物科技有限公司 | Needle mushroom aerated fermentation system with external pressure and temperature control functions |
| CN107739224A (en) * | 2017-10-17 | 2018-02-27 | 伊犁师范学院 | It is a kind of quickly to breed fluid nutrient medium of saussurea involucrata mushroom strains and preparation method thereof |
| CN113812302A (en) * | 2021-10-11 | 2021-12-21 | 中国农业科学院农业资源与农业区划研究所 | Method for preparing liquid edible fungus strain |
| CN114009272A (en) * | 2021-10-11 | 2022-02-08 | 中国农业科学院农业资源与农业区划研究所 | Liquid edible fungus culture solution and preparation method thereof |
| CN114027105A (en) * | 2021-11-23 | 2022-02-11 | 江苏久禾生物科技发展有限公司 | Culture method of high-protein pleurotus eryngii liquid strain |
| CN115024158A (en) * | 2022-06-20 | 2022-09-09 | 濮阳天耕农业科技有限公司 | Pleurotus eryngii liquid mother fungus culture method |
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| CN1440637A (en) * | 2003-04-01 | 2003-09-10 | 刘永昶 | Method and device for producing edible fungus liquid spawn |
| CN1774989A (en) * | 2005-12-02 | 2006-05-24 | 郑雪平 | Fully-coutrolled industrulized production method for king oyster mushroom |
| CN101066029A (en) * | 2007-06-05 | 2007-11-07 | 浙江大学 | Process of preparing colloidal edible fungus seed |
| CN101755600A (en) * | 2008-12-23 | 2010-06-30 | 上海高榕生物科技有限公司 | Method for cultivating apricot spore mushroom in single bottle |
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Patent Citations (5)
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| CN1362011A (en) * | 2002-02-01 | 2002-08-07 | 山东省农业科学院土壤肥料研究所 | Xinbao mushroom culturing method utilizing corn stalk |
| CN1440637A (en) * | 2003-04-01 | 2003-09-10 | 刘永昶 | Method and device for producing edible fungus liquid spawn |
| CN1774989A (en) * | 2005-12-02 | 2006-05-24 | 郑雪平 | Fully-coutrolled industrulized production method for king oyster mushroom |
| CN101066029A (en) * | 2007-06-05 | 2007-11-07 | 浙江大学 | Process of preparing colloidal edible fungus seed |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391034A (en) * | 2011-03-24 | 2012-03-28 | 上海雪榕生物科技股份有限公司 | Formula and preparation method of Pleurotus eryngii liquid strain culture medium |
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103155785B (en) * | 2011-12-09 | 2014-04-16 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN103416216A (en) * | 2012-05-23 | 2013-12-04 | 大连良农农业产业发展有限公司 | Formula for cultivating pleurotus eryngii liquid strain with corn stalks as base materials and preparation method thereof |
| CN103416216B (en) * | 2012-05-23 | 2016-01-20 | 大连良农农业产业发展有限公司 | Corn stalk makes formula of base-material Xinbao mushroom culturing liquid spawn and preparation method thereof |
| CN102845220A (en) * | 2012-09-10 | 2013-01-02 | 阜阳市德益农业科技有限公司 | Three-stage filtering ventilation device for liquid strain fermentation tank |
| CN103340094A (en) * | 2013-06-26 | 2013-10-09 | 襄汾县侯临农业科技有限公司 | Pleurotus eryngii liquid culturing method |
| CN104429583A (en) * | 2013-09-13 | 2015-03-25 | 孙君莲 | Edible fungus liquid strain culturing method |
| CN103922842A (en) * | 2014-04-17 | 2014-07-16 | 四川省砚山中药饮片有限公司 | Method for preparing pleurotus eryngii culture medium |
| CN104311348A (en) * | 2014-10-22 | 2015-01-28 | 常熟理工学院 | Improved liquid fermentation culture medium of pleurotus eryngii and method for culturing liquid strain of pleurotus eryngii by utilizing improved liquid fermentation culture medium |
| CN104311348B (en) * | 2014-10-22 | 2016-06-08 | 常熟理工学院 | The Pleurotus eryngii liquid fermentation medium improved and the method utilizing its cultivation pleurotus eryngii liquid strain |
| CN104604527A (en) * | 2015-02-11 | 2015-05-13 | 北京华绿生物科技有限公司 | Needle mushroom aerated fermentation system with external pressure and temperature control functions |
| CN107739224A (en) * | 2017-10-17 | 2018-02-27 | 伊犁师范学院 | It is a kind of quickly to breed fluid nutrient medium of saussurea involucrata mushroom strains and preparation method thereof |
| CN113812302A (en) * | 2021-10-11 | 2021-12-21 | 中国农业科学院农业资源与农业区划研究所 | Method for preparing liquid edible fungus strain |
| CN114009272A (en) * | 2021-10-11 | 2022-02-08 | 中国农业科学院农业资源与农业区划研究所 | Liquid edible fungus culture solution and preparation method thereof |
| CN114027105A (en) * | 2021-11-23 | 2022-02-11 | 江苏久禾生物科技发展有限公司 | Culture method of high-protein pleurotus eryngii liquid strain |
| CN115024158A (en) * | 2022-06-20 | 2022-09-09 | 濮阳天耕农业科技有限公司 | Pleurotus eryngii liquid mother fungus culture method |
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Application publication date: 20110112 |