CN1774989A - Fully-coutrolled industrulized production method for king oyster mushroom - Google Patents
Fully-coutrolled industrulized production method for king oyster mushroom Download PDFInfo
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- CN1774989A CN1774989A CN 200510101815 CN200510101815A CN1774989A CN 1774989 A CN1774989 A CN 1774989A CN 200510101815 CN200510101815 CN 200510101815 CN 200510101815 A CN200510101815 A CN 200510101815A CN 1774989 A CN1774989 A CN 1774989A
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Abstract
The present invention discloses a fully-controlled industrialization production method of Xingbao mushroom, belonging to the field of edible mushroom cultivation technology. Said method includes the following steps: inoculum preparation, making mushroom bag, inoculum culture, bud forcing treatment, mushroom growth, collecting, sorting and packaging, etc.
Description
Technical field
The present invention relates to cultivation technology of edible fungi, refer in particular to a kind of full control industrial production method of Xingbao mushroom.
Background technology
Traditional planting almond abalone mushroom is the natural environment that utilizes winter, cultivates in plastic tunnel, this mode Xinbao mushroom culturing exist yield poorly, the quality instability, be subjected to effect of natural conditions big, be difficult to satisfy the needs in market.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of full control industrial production method that is not subjected to a kind of Xingbao mushroom of environmental condition restriction, constant product quality is provided.
The object of the present invention is achieved like this: a kind of full control industrial production method of Xingbao mushroom, comprise that bacterial classification is made, mushroom packs work, bacteria, urge flower bud, fruiting, gather, classification and packing, wherein: described bacterial classification is made, mushroom packs work, bacteria, urge flower bud, fruiting is all finished under the working condition of full control.
Bacterial classification described in the full control industrial production method of above-mentioned a kind of Xingbao mushroom is made and is adopted the bamboo let bacterial classification, and the culture of strains condition is: 20～28 ℃ of temperature, relative air humidity 50～70%, gas concentration lwevel 2000～7500ppm.
During bacteria described in the full control industrial production method of above-mentioned a kind of Xingbao mushroom, the mushroom bag is put into the screen cloth brandreth, mushroom bag emission intensity 200～250 bag/m
2, each mushroom bag is separated from each other condition of culture: 20～28 ℃ of temperature, relative air humidity 50～70%, gas concentration lwevel 2000～7500ppm.
The flower bud condition of urging described in the full control industrial production method of above-mentioned a kind of Xingbao mushroom is: 10～19 ℃ of temperature, relative air humidity 70～95%, gas concentration lwevel 800～4500ppm, keep 300～1000Lx illumination, the sack of mushroom bag pulls out cotton, DNA releaxed circular DNA to sack, a bag end and opens " V " shape mouth, up to forming the mushroom flower bud, when treating that the mushroom body grows to 0.5～2.5cm, open bag, dredge flower bud.
Fruiting condition described in the full control industrial production method of above-mentioned a kind of Xingbao mushroom is: 10～20 ℃ of temperature, relative air humidity 85～95%, gas concentration lwevel 800～3500ppm, illumination 100Lx.
The present invention compared with prior art has following advantage:
1. the steady quality of Xingbao mushroom, quality better;
2. the output height can guarantee the whole fruitings of cultivation bag, and single bag can be produced more than the Xingbao mushroom 300g.
3. be not subjected to the influence of natural conditions, can produce the whole year.
Description of drawings
Below in conjunction with drawings and Examples the present invention is described in further detail, but does not constitute any limitation of the invention.
Fig. 1 is a process flow diagram of the present invention.
Embodiment
Consult shown in Figure 1, the full control industrial production method of a kind of Xingbao mushroom of the present invention, its technological process is that bacterial classification making → mushroom packs work → bacteria → urge flower bud → fruiting → gather → classification → packing, details are as follows to each step below in conjunction with specific embodiment.
Embodiment
One, bacterial classification is made
1. the making of first class inoculum
(1) preservation bacterial classification prescription: water 1000ml, potato 200g, glucose 20g, agar 25g.
(2) produce the bacterial classification prescription: water 1000ml, potato 200g, glucose 20g, corn flour 20g, KH
2PO
41.0g, MgSO
40.5g, spray precious 4.
(3) preparation method: with peeling potatoes, clean back shave, add water boil to soft and not mashed, cross leaching juice, then agar 25g is added and boil to whole thawings, filtration adds water to 1000ml, adds other material in the prescription at last, is divided in the test tube after stirring is dissolved, every test tube packing medium 15ml makes tampon immediately.
(4) sterilization: the test tube that branch is installed is put into sterilizer with the steam sterilization sterilization, and condition is: steam pressure reaches 0.5kg/cm
2The time steam bleeding emptying, and then in 1.2kg/cm
2Kept 45 minutes during steam pressure, be cooled to 60 ℃ and begin to put the inclined-plane, solidify back dehumidifier in 4 ℃ of refrigerators, use after three days.
(5) inoculation: select the one-level kind of preservation or introduction and test tube slant to be connect, alcolhol burner, 75% alcohol, match transfer needle etc. to put into inoculating hood in the lump, light sterilization 30 minutes, be used for changeing expansion one-level kind with aerosol 4g.
(6) cultivate: will inoculate good inclined-plane and put into 25 ℃ of culturing room's cultivations, Xingbao mushroom covered with in 12～15 days, used in 1～2 day behind the full packages, and the one-level kind that can not at once use is put into 4 ℃ of refrigerator preservations.
2. the making of secondary bamboo let kind
(1) get the raw materials ready: take by weighing the long bamboo let of having rived of 12cm, soak 24h with 2% limewash, the back with flushing with clean water to clearly and drain excessive moisture.
(2) spice: press bamboo let 20kg, wheat skin 6kg, wood chip 8kg, CaCO
30.05kg, water 16kg mixes material thoroughly, in the 250ml wide-mouth bottle of packing into then, every bottled 40～45 bamboo lets, 1cm is thick for loam cake wood chip compound, after fill up wood chip compound and washer bottle wall and bottleneck, filled in tampon.
(3) sterilization: the wide-mouth bottle that will install composts or fertilisers of cultivating is put into the high steam pot and is carried out steam sterilizing, condition: boost to 0.5kg/cm
2Steam discharge and then boost to 0.5kg/cm
2Steam discharge boosts to 2kg/cm at last
2Keep 2h, the oven dry tampon of uncapping after the cooling takes the dish out of the pot.
(4) inoculation: identical with the inoculation during first class inoculum is made.
(5) cultivate: put into 20～28 ℃ of temperature, relative air humidity 50～70%, the culturing room of gas concentration lwevel 2000～7500ppm cultivated 10 days, look into bottle every three days once, checked once every five days after 10 days, assorted bacterium such as discovery Mucor, green mold will be sorted out immediately and destroy, and can expire bottle after 35 days and use.The spawn culture chamber should be every 10 days with aerosol and 1～3% Lysol or the sterilization in turn of 0.25% benzalkonium bromide.
Two, mushroom packs work
(1) prescription: boll hull 55%, wood chip 20%, wheat skin 18.5%, corn flour 5%, sugar 0.5%, CaCO
31%, material-water ratio 1: 1.6, water content 65%, 3.0 ± 0.1 jin of 1.15 ± 0.5 jin, single bag weight in wet bases of packed siccative, pH value 9.0.
(2) pre-heap: first wood chip with requirement looses and piles up thick 25cm on the ground, mix wet by 1: 1.6～1.8 material-water ratios required cotton seed hull again, it is thick to be spread on the last 15～20cm of wood chip heap, again wood chip is laid on the cotton seed hull, evenly add water again according to the wet situation combination of doing of material, after stopping 30 minutes, will expect to beat even with the dnockout machine.
(3) play heap: will beat even compound and stack by 200 * 100～120cm, length is not limit, pile material after, on material, evenly burrow bottom the material, during the thermometer insertion is expected by 30 * 30cm.
(4) turning: when temperature reaches 58～62 ℃, carry out the turning first time, during turning the material on surface is put into the centre, above the material of bottom is put into, the middle material surface of taking precautions against natural calamities, do heap by same heap shape again, burrow, after temperature rises to 58 ℃ once more, carry out the turning second time, method of operating 2～3 times like this, is finished fermentation with for the first time.
(5) spice: the material that will ferment is put into the dnockout machine, and the auxiliary material that adds in all the other prescriptions stirs, and measures water content, and with holding material, the webs water breakthrough will drip but not drip and get final product.
(6) packing: material is changed in the cargo jack, and in 17 * 38 * 0.05 polypropylene plastic sack of by weight material being packed into, the collar, plug tampon uprightly neatly are emitted on bag in the chase then.
(7) heap stove: charged pocket chase is stacked the face top that overarches in stove, cover the waterproof plastic film, use rope fixing behind film, the colour bar cloth greatly, press bottom periphery, anti-leak-stopping vapour.
(8) sterilization: get final product generation steam sterilizing on fire, time 10h after stacking is finished.
(9) come out of the stove: after sterilization finishes, treat colour bar cloth, big film, waterproof plastic film to be taken off when temperature drops to 85～90 ℃, temperature drops to 65～70 ℃, cultivation bag can be transported in the transfer room to cool off.
(10) inoculation: use alcohol wipe bacterial classification bottleneck and inwall during inoculation, the wood chip kind on bottle upper strata is dialled pine, insert inner firmly rotation of bottle with tweezers again and make the bamboo let kind loose, cultivation bag to be inoculated is uprightly put well to a direction, pull up tampon, press from both sides out a bamboo let kind immediately, insert the reservation hole in the cultivation bag, dial one deck wood chip bacterial classification capping down at once, cotton plug beyond the Great Wall, the ring of helping pull a cart back and forth shakes cultivation bag, allows the wood chip bacterial classification front cover that scatters, and can finish one bag of inoculation work.
Three, bacteria
(1) preparation in cultivation room, after every batch of bacterium bag cultivation is finished, promptly regularly cultivate the irrigation and disinfection in room, general earlier with jetting machine flushing, cleaning, wall, smallpox, ground are washed with 5～10% limewash in the back, fumigate by per 2～4g/m3 aerial fog disinfectant again, seal 12～24 hours waste gas that disperses of can windowing.
(2) will inoculate good cultivation bag together with the accumbency of transhipment chase, be sidelong into culturing room, just determine porousness according to temperature, to guarantee that temperature between the cultivation bag is below 28 ℃, condition of culture: 20～28 ℃ of temperature, relative air humidity 50～70%, gas concentration lwevel 2000～7500ppm, the ventilation of needn't windowing before 10 days, sprayed with Venus thimerosal or lysol space every 5 days, after 15 days every morning 5:00～7:00 and afternoon 17:00～19:00 door window ventilated 10～20 minutes, selecting the pollution bag every other day also in time goes out clearly, general Xingbao mushroom can both can produce culturing room 35 days pursefuls.
Four, urge flower bud
(1) package is put on the shelf: will successively be emitted on the cultivation rack of fruiting room (or freezing the storehouse) through the cultivation bag after culturing room's bacteria is finished, the spacing distance between bag and the bag is 1cm.
(2) urge flower bud: the fruiting room is controlled at 10～19 ℃ of temperature, relative air humidity 70～95%, gas concentration lwevel 800～4500ppm, keep 300～1000Lx illumination, the sack of cultivation bag is dialled cotton, DNA releaxed circular DNA to sack, a bag end and is opened " V " shape mouth, up to forming the mushroom flower bud, when treating that the mushroom body grows to 0.5～2.5cm, open bag, dredge groove.
Five, fruiting
The fruiting room is controlled at 10～20 ℃ of temperature, relative air humidity 85～95%, gas concentration lwevel 800～3500ppm, illumination 100Lx.
Six, gather
The Xingbao mushroom of fruiting room cultivation generally began to gather after 14 days, criticized mushroom to the 20th top margin of a page and gathered and finish, and the fruiting room is controlled at 10～20 ℃ of temperature, relative air humidity 85～95%, gas concentration lwevel 800～3500ppm, illumination 100Lx.
Seven, classification
Length, diameter, weight by Xingbao mushroom are classified.
Eight, packing
With similar Xingbao mushroom quantitatively put into plastic sack, the carton of packing into after sealing.
Claims (5)
1. the full control industrial production method of an Xingbao mushroom, comprise that bacterial classification is made, mushroom packs work, bacteria, urge flower bud, fruiting, gather, classification and packing, it is characterized in that: described bacterial classification is made, mushroom packs work, bacteria, urge flower bud, fruiting is all finished under the working condition of full control.
2. method according to claim 1 is characterized in that: described bacterial classification is made and is adopted the bamboo let bacterial classification, and the culture of strains condition is: 20～28 ℃ of temperature, relative air humidity 50～70%, gas concentration lwevel 2000～7500ppm.
3. method according to claim 1 is characterized in that: during described bacteria, the mushroom bag is put into the screen cloth brandreth, mushroom bag emission intensity 200～250 bag/m
2, each mushroom bag is separated from each other condition of culture: 20～28 ℃ of temperature, relative air humidity 50～70%, gas concentration lwevel 2000～7500ppm.
4. method according to claim 1, it is characterized in that: the described flower bud condition of urging is: 10～19 ℃ of temperature, relative air humidity 70～95%, gas concentration lwevel 800～4500ppm, keep 300～1000Lx illumination, the sack of mushroom bag pulls out cotton, DNA releaxed circular DNA to sack, a bag end and opens " V " shape mouth, up to forming the mushroom flower bud, when treating that the mushroom body grows to 0.5～2.5cm, open bag, dredge flower bud.
5. method according to claim 1 is characterized in that: described fruiting condition is: 10～20 ℃ of temperature, relative air humidity 85～95%, gas concentration lwevel 800～3500ppm, illumination 100Lx.
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| CNB200510101815XA CN100356835C (en) | 2005-12-02 | 2005-12-02 | Fully-coutrolled industrulized production method for king oyster mushroom |
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|---|---|---|---|
| CNB200510101815XA CN100356835C (en) | 2005-12-02 | 2005-12-02 | Fully-coutrolled industrulized production method for king oyster mushroom |
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| CN100356835C CN100356835C (en) | 2007-12-26 |
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| CN101940129A (en) * | 2010-09-06 | 2011-01-12 | 阜阳市德益农业科技有限公司 | Method and device for preparing pleurotus eryngii liquid strain |
| CN101611684B (en) * | 2008-06-25 | 2011-05-18 | 上海市农业科学院 | Primordium control method during pleurotus eryngiu factory production. |
| CN102197761A (en) * | 2011-03-29 | 2011-09-28 | 福建绿宝食品集团有限公司 | Method for culturing pleurotus eryngii |
| CN102265754A (en) * | 2011-06-24 | 2011-12-07 | 郑雪平 | Pleurotus eryngii factory bottle cultivation method |
| CN102487728A (en) * | 2011-12-26 | 2012-06-13 | 湖南省春华生物科技有限公司 | Bag-cultivated angle pulling bud promoting method for producing pleurotus errngil |
| CN103430772A (en) * | 2013-08-29 | 2013-12-11 | 赵广峰 | Comprehensive factory-like circulation production method of pleurotus eryngii and common edible mushrooms |
| CN103875457A (en) * | 2014-04-16 | 2014-06-25 | 山西奥格姆农业科技有限公司 | Production method for improving fruiting quality of bagged cultivated pleurotus eryngii |
| CN104106373A (en) * | 2014-06-30 | 2014-10-22 | 绿宝生态农业（漳州）有限公司 | Fruiting method for pleurotus eryngii |
| CN104115675A (en) * | 2014-08-21 | 2014-10-29 | 湖南省宇秀生物科技有限公司 | Method for cultivating bottle-planted Pleurotus eryngii in factory-like mode |
| CN104285677A (en) * | 2014-10-30 | 2015-01-21 | 武汉岁岁丰农业科技开发有限公司 | Method for making edible mushroom pegwood strain |
| CN107667777A (en) * | 2017-09-30 | 2018-02-09 | 重庆维得鲜农业发展有限公司 | A kind of pleurotus eryngii cultural method |
| CN108739051A (en) * | 2018-05-25 | 2018-11-06 | 江苏香如生物科技股份有限公司 | A kind of pleurotus eryngii fruiting cultural method |
| CN111066569A (en) * | 2019-12-18 | 2020-04-28 | 江苏沿江地区农业科学研究所 | Method for producing pleurotus eryngii with fresh and fragrant taste |
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| CN1166272C (en) * | 2002-02-01 | 2004-09-15 | 山东省农业科学院土壤肥料研究所 | Xinbao mushroom culturing method utilizing corn stalk |
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- 2005-12-02 CN CNB200510101815XA patent/CN100356835C/en not_active Expired - Fee Related
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| CN101611684B (en) * | 2008-06-25 | 2011-05-18 | 上海市农业科学院 | Primordium control method during pleurotus eryngiu factory production. |
| CN101940129A (en) * | 2010-09-06 | 2011-01-12 | 阜阳市德益农业科技有限公司 | Method and device for preparing pleurotus eryngii liquid strain |
| CN102197761A (en) * | 2011-03-29 | 2011-09-28 | 福建绿宝食品集团有限公司 | Method for culturing pleurotus eryngii |
| CN102197761B (en) * | 2011-03-29 | 2012-10-24 | 福建绿宝食品集团有限公司 | Method for culturing pleurotus eryngii |
| CN102265754A (en) * | 2011-06-24 | 2011-12-07 | 郑雪平 | Pleurotus eryngii factory bottle cultivation method |
| CN102265754B (en) * | 2011-06-24 | 2013-01-30 | 郑雪平 | Pleurotus eryngii factory bottle cultivation method |
| CN102487728A (en) * | 2011-12-26 | 2012-06-13 | 湖南省春华生物科技有限公司 | Bag-cultivated angle pulling bud promoting method for producing pleurotus errngil |
| CN102487728B (en) * | 2011-12-26 | 2013-06-19 | 湖南省春华生物科技有限公司 | Bag-cultivated angle pulling bud promoting method for producing pleurotus errngil |
| CN103430772B (en) * | 2013-08-29 | 2014-11-05 | 赵广峰 | Comprehensive factory-like circulation production method of pleurotus eryngii and common edible mushrooms |
| CN103430772A (en) * | 2013-08-29 | 2013-12-11 | 赵广峰 | Comprehensive factory-like circulation production method of pleurotus eryngii and common edible mushrooms |
| CN103875457A (en) * | 2014-04-16 | 2014-06-25 | 山西奥格姆农业科技有限公司 | Production method for improving fruiting quality of bagged cultivated pleurotus eryngii |
| CN103875457B (en) * | 2014-04-16 | 2015-10-28 | 山西奥格姆农业科技有限公司 | A kind of production method improving bag cultivation pleurotus eryngii fruiting quality |
| CN104106373A (en) * | 2014-06-30 | 2014-10-22 | 绿宝生态农业（漳州）有限公司 | Fruiting method for pleurotus eryngii |
| CN104106373B (en) * | 2014-06-30 | 2016-04-06 | 绿宝生态农业（漳州）有限公司 | A kind of fruiting method of Xingbao mushroom |
| CN104115675A (en) * | 2014-08-21 | 2014-10-29 | 湖南省宇秀生物科技有限公司 | Method for cultivating bottle-planted Pleurotus eryngii in factory-like mode |
| CN104115675B (en) * | 2014-08-21 | 2015-12-02 | 湖南省宇秀生物科技有限公司 | A kind of industrial bottle plants the method that Xingbao mushroom educates mushroom |
| CN104285677A (en) * | 2014-10-30 | 2015-01-21 | 武汉岁岁丰农业科技开发有限公司 | Method for making edible mushroom pegwood strain |
| CN107667777A (en) * | 2017-09-30 | 2018-02-09 | 重庆维得鲜农业发展有限公司 | A kind of pleurotus eryngii cultural method |
| CN108739051A (en) * | 2018-05-25 | 2018-11-06 | 江苏香如生物科技股份有限公司 | A kind of pleurotus eryngii fruiting cultural method |
| CN111066569A (en) * | 2019-12-18 | 2020-04-28 | 江苏沿江地区农业科学研究所 | Method for producing pleurotus eryngii with fresh and fragrant taste |
| CN111066569B (en) * | 2019-12-18 | 2021-11-05 | 江苏沿江地区农业科学研究所 | Method for producing pleurotus eryngii with fresh and fragrant taste |
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