CN1372786A

CN1372786A - Efficient industrial cultivation technique of gastrodia tuber

Info

Publication number: CN1372786A
Application number: CN 01145550
Authority: CN
Inventors: 崔学发
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-08-08
Filing date: 2001-12-31
Publication date: 2002-10-09

Abstract

An efficient industrial technology for cultivating gastrodia tuber is characterized by that the solid, semi-solid and liquid culture media are artificially prepared for culturing shoestring fungus used in the cultivation of gastrodia tuber, and the each phase and each link in said cultivation are intelligently monitored and managed to obtain high quality and yield of gastrodia tuber.

Description

The efficient industrial cultivation technique of rhizoma Gastrodiae

The aseptic efficiency cultivation technology for high of the batch production of rhizoma Gastrodiae, invention relates to utilize the medium and the sterile working production technology of artificial preparation, cultures the aseptic efficient cultivation rhizoma Gastrodiae technology of a kind of batch production that armillaria mellea is realized.

Rhizoma Gastrodiae is a kind of orchid, and stem tuber can be made medicine.Because the rhizoma Gastrodiae unrooted does not have leaf, itself can not absorb nutrition from the external world, and it must rely on a kind of fungi supply nutrition, finish it and grow, otherwise will be dead.At present no matter be the rhizoma Gastrodiae that obtains of nature or tame, its cultivation method is: after utilizing the forest section of being sawn into of new felling, armillaria mellea growth substrate (nutrient source) is provided, under normal condition (hill, field) or be contained in sack, the chest and cultivate rhizoma Gastrodiae, this cultivation method has just simply imitated rhizoma Gastrodiae at the natural world growth conditions; Trees are in process of growth, and the needed nutriment of armillaria mellea has been stored up in inside, and armillaria mellea, obtains energy and various nutriment and finishes its growth constantly from decompose the organic matter that absorbs bark inside by metabolism.The nutriment of bark inside has not only satisfied armillaria mellea growth requirement, and bark is also more stable in discharging the process of decomposing the idiotrophic energy, has met the requirement of armillaria mellea long term growth.Therefore, also satisfied the growth requirement of this special plant of rhizoma Gastrodiae.Along with the continuous increase of rhizoma Gastrodiae demand, causing cuts down forest in a large number is used for rhizoma Gastrodiae production now, and this damages by original very fragile natural ecological environment for us.In order to change this present situation, also the someone utilizes fruit tree branch, leaf to bring up armillaria mellea and cultivates rhizoma Gastrodiae, and the method for this cultivation rhizoma Gastrodiae also is simply to have imitated prior art, does not have fundamentally to change the way of cultivation rhizoma Gastrodiae with forest.In addition, just cultivating the rhizoma Gastrodiae technology at present, be used to cultivate the medium of armillaria mellea, all is to be in for a long time under the nature normal condition, and medium is not taken precautions against ability, and harmful bacterium can directly go into medium (trees) inside for a long time.Therefore, the inner nutrition major part of medium (trees) is eaten by other assorted bacterium, and its nutrition can not effectively utilize.

In order to change the deficiencies in the prior art, the inventor is to China Intellectual Property Office, with regard to this art applications twice patent application file, for the first time on December 9th, 2000, application number is 00134935.X, name is called " factory culture of rhizoma Gastrodiae "; Improve for the second time and apply at August 8 calendar year 2001, application number: 01125303.7, name is called " the workerization efficiency cultivation technology for high of rhizoma Gastrodiae ", by improving, though fundamentally changed existing cultivation rhizoma Gastrodiae technology, also has certain deficiency; This improvement is exactly very the deficiency that last twice application documents proposed to be improved and perfect.

Purpose of the present invention is exactly at the deficiencies in the prior art and application number: 00134935.X and application number: the deficiency that 01125303.7 file is proposed is improved, and utilizes state-of-the-art technology, equipment, passes through intelligent management; Thereby make gastrodin cultivation production reach the aseptic efficient cultivation rhizoma Gastrodiae production technology of a kind of batch production.

The technical method and the production technology content of invention include following:

1, culture medium preparation:

The medium of artificial preparation is different from trees, its processing of must preparing burden, the various materials used to medium carry out rational nutrition-allocated proportion, can be mixed with medium various dissimilar, to satisfy the feature request that armillaria mellea is grown.

Selecting for use of medium material: available various farmlands straw, bar (as: corncob, corn stalk, careless straw, beanstalk or the like), various " shells " (as: peanut shell, cotton seed hull etc.), also available crops and agricultural byproducts are as corn flour, bean powder, wheat bran, potato, sugar or the like, also available forest, shrub, sugared slag, according to foam, weed tree sawdust, some being rich in nutrition materials such as branch, leaf all can be used as the medium material; Medium also can add the various suitable chemical elements that have.

2, the processing of medium: the various materials that medium is used, can carry out pulverization process, can not pulverize yet; Various medium materials can use separately, also can be to the material of various medium, and what be allowed a choice carries out the energy rational proportion.Can be the material that is used for medium, be formulated as multiple different prescription, dissimilar; Make it medium and reach that nutrition is complete, C/N ratio is reasonable.Can add some auxiliary materials such as gypsum, lime when the preparation medium, make the medium pH value about 5.0---6.0, humidity can be between 50---75%.After culture base-material is deployed, with artificial or machine, in the instruments such as cultivation bag, case that culture base-material is packed into.

Medium also can be according to the various different requirements of cultivation rhizoma Gastrodiae production technology, culture base-material be processed into various dissimilar as: pulverize the loose shape in back, solid, semisolid (semiliquid) or the like.

The method that a, medium are processed into solid is: prepared culture medium material (already pulverised or do not have the material pulverized), can add bonding Ji (also can not add colloid) such as colloid, carrying out extrusion processing is various bodies, by extrusion processing, the various materials of medium are condensed in together mutually, reach certain intensity, density, extruding can utilize special mechanical equipment or artificial.Processed good solid culture medium, other method such as also available various mesh bags, plastic bag with holes, paper bag is packed and is cultivated rhizoma Gastrodiae.

B, medium are processed as semi-liquid method: can select for use one or more medium materials to carry out rational proportion, make the semiliquid formula medium of number of different types; Making in the middle of the semiliquid formula medium material, can be selected into after crushed potato, pachyrhizus, agar, corn flour, bean powder, taro etc. in right amount, making the medium after its processing, can reach a kind of paste, freeze the semi-liquid stage of shape.As: the number of different types agar medium that processes with other prescriptions such as agar, potato, sugar, wood chips; Also can utilize multiple different medium material as: utilizing has suitable chemical element, formulated to go out multiple multi-form semisolid culturemedium; The semiliquid medium that processes can be packed into instruments such as cultivation bag, casees, and method such as also available various mesh bags, plastic bag with holes, paper bag is packed.

C, in order to strengthen the harmful not infected ability of bacterium of medium opposing, when the various medium of processing, can needs be formulated in the middle of the medium material easily by the material of microbial contamination and nutrient content higher material as corn flour, bean powder, potato or the like material, be formulated in the central core of medium; The setting of medium external surface layer is not easy by the material of microbial contamination or nutrient content less substance as the farmland straw of pulverizing, bar, wood chip, paper bag, soil, husky or the like; The medium internal layer is wrapped up; Medium can be effectively reduced like this by the chance of microbial contamination, medicines such as the anticorrosion materials that are used for various food, sterilization spirit can also be added in medium inside in order to strengthen the anti-sense ability of medium.

The purpose that d, medium are processed into solid is: make medium reach certain intensity, make things convenient for production operation, cultivation, make nutrition concentrate, decompose slowly, reduce spatial volume, strengthen the medium anti-infection ability, allow the shoestring body of most of armillaria mellea, be grown in the outside of solid culture medium, be fit to rhizoma Gastrodiae growth requirement, allow rhizoma Gastrodiae intercept and capture more shoestring body.Medium is processed as another purpose of solid: utilize the solid culture medium that processes, also can carry out cultivating rhizoma Gastrodiae under normal condition.Medium is processed as semi-liquid purpose: can be used for cultivating the provenance of armillaria mellea, can carry out the bacterium material procreation of medium again, can directly carry out the cultivation and production of rhizoma Gastrodiae again when the bacterium material.In addition, medium is processed into semisolid purpose and also is: change the existence and the supply mode of medium internal moisture, nutrition, more help absorption, the growth procreation of armillaria mellea.The purpose that medium is processed as loose shape is: pulverized back medium material, help the absorption of armillaria mellea on the one hand, two can be used to be used as the cultivation thing of rhizoma Gastrodiae again.

3, the setting of cultivation bag, the aseptic ventilation hole of case: armillaria mellea has the function of drawing oxygen in process of growth.Therefore, cultivation bag, case will be provided with aseptic vent device, are used for gas exchange in bag, the case.The various medium that process are behind the instruments such as the cultivation bag of packing into, case, at the sack or the chest sealing part (feeding mouth place) of cultivation rhizoma Gastrodiae, a ring is set, sack is nested in the ring, sack upset part outside ring, with a lid that matches, be pressed on the ring closely again; Make cultivation bag and ring, closely cooperate by " lid " formation.On lid, also be provided with, one or two ventilation holes (pipeline), in the hole, can be provided with, the gas in the sack is exchanged, the device that can prevent the harmful bacteria invasion and attack again as; Cotton, sponge, mistake bacteriological filtration paper or the like.The ventilation hole that is provided with on cultivation bag, the case also can directly seal with objects such as cotton, sponges.Needed gas in the cultivation bag, case also can pass through pipeline (ventilation hole) and directly import filtrated air behind disinfection filtering.

4, medium sterilization operation: the various types of medium that will prepare, process after installing with apparatus such as cultivation bag, casees, can enter the sterilization production process.Sterilization available dedicated equipment is carried out high temperature high pressure sterilizing as boiler, micro-wave oven, and other method such as the also available medicine of sterilization, fermentation is implemented.

5, filtrated air filtration, purification special equipment device:

After medium is finished sterilization process, can carry out the aseptic cultivation of bacterial classification of armillaria mellea and the aseptic culture production process of procreation and rhizoma Gastrodiae, production operation can be carried out at the set aseptic transparent operation case of filtrated air filtering and purifying, and this operation also can be carried out under aseptic conditions such as desinfection chamber, sterile board.Filtrated air filtration, purification production equipment special device: optional compressor or the blower fan (4) got, can be at inlet, the port of export of blower fan, be provided with seal case (3), the output of air disinfection soup and two air, input channel are housed in seal case (3), blower fan (4) is by being arranged on the pipeline in the sealing spray tank (3), can suck air or be blown into soup inside,, kill the harmful bacteria in the air the gas bacterium of filtering, purify, disappear; The sterilization of air also can be arranged on an opaque casing (1), and one or several ultraviolet sterilizing lamps (6) can be installed in casing (1) inside; In order to strengthen sterilizing ability, also can be provided with refraction reflection function good object as objects such as eyeglasses at cabinet wall to air; Make cabinet wall bright and clean (2), can increase bactericidal effect like this air; Air in the case is after sterilization, and available blower fan sucks or be blown into sterile production operational sequence (in the control box) by the pipeline that is arranged on the case.More than two kinds of technical methods to air disinfection, filtration, purification: can contact separately, be arranged in parallel, two kinds of method for disinfection also can carry out series, parallel simultaneously to be used; And can be arranged on the outlet or the arrival end of blower fan or compressor (4); By blower fan or compressor (4), the aseptic gas behind the disinfection filtering, being transported in the sterile production operational sequence or being transported to provides rhizoma Gastrodiae, armillaria mellea to use in cultivation bag, the case.The sterile production operational sequence, be arranged on a transparent seal operation casing (if use machine operation, casing can be provided with arbitrarily) in, be filled aseptic gas in the case, (at the box house of sterile working, also ultraviolet germicidal lamp can be set), be provided with seal operation hole (7) on operation casing both sides, in the hole, devices such as rubber gloves or manipulator can be installed; Handle hole also can be set to semi-open; In sterile working case production process part, also be provided with transmission device (8), be used for the output and the input of medium; The turnover of medium also can be finished by the semi-open handle hole that is provided with; In the various sterile working production processes of gastrodin cultivation as: gastrodin cultivation, armillaria mellea are cultivated, the procreation of bacterium material, and be available artificial or finish at the sterile working case with machine.At the transparent operation box back that the sterile working production process is provided with, be provided with a sealing sliding door (10) that can open, the turnover that is used to expect; When door was outwards pulled out, door can enter in the protection door pocket (9) of setting.

6, the aseptic cultivation and the procreation of armillaria mellea bacterial classification:

The aseptic cultivation of a, armillaria mellea bacterial classification: this operation can be carried out in the set sterile working production process (transparent operation casing) of filtrated air filtration, purification special equipment device, also can carry out under the aseptic conditions of work such as sterile board, superclean bench at desinfection chamber; Select the children tender, thick, healthy, armillaria mellea is had the strong part of survival ability as far as possible, have the shoestring body integral body of the old and new's armillaria mellea to take off long, the sterilization processing that carries out disinfection is killed attached to the bacterium of mixing that is harmful on the armillaria mellea.If the shoestring body is the medication liquid disinfectant, available sterile water is removed soup and is wiped the solid carbon dioxide branch; Under aseptic condition, insert manufactured, pass through sterilization process, semiliquid medium inside; As: multiple agar medium that makes with various prescriptions, type or the semiliquid medium that makes with other method.After semisolid culturemedium inserted bacterial classification, instruments such as available gastrodin cultivation bag, bottle, chest were packed into, after the aseptic sealing, are transferred to culturing room from operating room, carry out the bacterial classification aseptic culture of armillaria mellea; Culturing room requires that uniform temperature is arranged, Controllable Temperature built in about the 13-28 degree, the suitable shading of medium.Gas in cultivation bag, the case can be finished by being arranged on the sealing ventilation hole that covers, also can utilize the pipeline input to offer armillaria mellea (the aseptic cultivation of armillaria mellea bacterial classification also can be arranged on and carry out in the various cultivation apparatus cultivating under the air-proof condition) through the filtrated air of filtrated air filtering and purifying.Armillaria mellea shoestring body is in the semiliquid medium, after the certain hour cultivation, armillaria mellea will be at the inner a large amount of shoestring body that makes new advances of multiplying of medium, the aseptic armillaria mellea bacterial classification that cultivates by this method, can be used for carrying out the procreation of the bacterium material (bacterium base) of all kinds medium, also can directly be used for the cultivation and production of rhizoma Gastrodiae as the bacterium material.(the shoestring body of the armillaria mellea that cultivates with this method also can be planted the rhizoma Gastrodiae technology to existing, carries out the improvement of armillaria mellea and regenerates).The aseptic cultivation of armillaria mellea bacterial classification and procreation also can be passed through: female plant separate, female plant cultivate, original seed is cultivated the back, when nascent mycelial growth is the shoestring body, the cultivated species of practicable armillaria mellea is cultivated, after medium is multiplied through the bacterium material, cultivate the method realization that rhizoma Gastrodiae is produced again.

The bacterium material of b, armillaria mellea (bacterium base) procreation: after the aseptic breeding strain of armillaria mellea is good, can be according to the condition of production, the various medium that process are carried out the bacterium material of armillaria mellea and multiply production process, the production operation operation can be carried out under the aseptic conditions such as sterile working glass box of filtrated air filtering and purifying setting; Operate available artificial or machine enforcement; What prepare, process, various types of medium of sterilization process have been finished; As: various materials through pulverizing the prescription back in bulk in sack, chest medium or the solid that processes, semi-liquid medium or the forest that need not pulverize, shrub, straw, bar etc. are used to cultivate the material of rhizoma Gastrodiae, the various medium that need carry out the bacterium material procreation of armillaria mellea, available various apparatus or cultivation bag, case are packed into.Choose not by living contaminants, healthy, the armillaria mellea that has nutrient source (bacterium material, bacterium base), under aseptic condition, choosing to put in right amount needs the various medium of procreation to inoculate procreation, after the culture medium inoculated sealing, change bacterium base (bacterium material) procreation that culturing room carries out the medium armillaria mellea over to.The bacterium material of armillaria mellea (bacterium base) cultivating method and culture technology require identical with the aseptic breeding method of armillaria mellea bacterial classification.After medium is cultivated through certain hour,, when multiplying on the needed medium regular period, multiply the bacterium material (medium) of getting well, be can be used for cultivating rhizoma Gastrodiae production when the shoestring body of armillaria mellea.The procreation of the cultivation of armillaria mellea and bacterium material, also can be with after gathering in the crops rhizoma Gastrodiae last year, medium still have existence reasonable, not by living contaminants, healthy armillaria mellea medium, aseptic condition takes out down, be used as the cultivation and the procreation of next round armillaria mellea, also can directly be used for the cultivation and production of rhizoma Gastrodiae lower whorl as the bacterium material.

7, the aseptic culture production of rhizoma Gastrodiae:

After the bacterium base of armillaria mellea (bacterium material) procreation is good (cultivation of rhizoma Gastrodiae also can be carried out simultaneously with bacterium base, the procreation of bacterium material of armillaria mellea), gastrodin cultivation is not subjected to seasonal restriction, can carry out gastrodin cultivation production at any time according to the condition of production.The gastrodin cultivation production process, being arranged on sterile working production case carries out, cultivation rhizoma Gastrodiae needed various medium, needed various apparatus as cultivation bag, the various medium that process, bacterium material, rhizoma Gastrodiae kind, thimerosal or the like apparatus, are put into and carried out aseptic process in the sterile production control box.Under aseptic condition, manually can utilize to be arranged on that handle hole carries out sterile working production on the glass box, also can utilize the special purpose machinery apparatus, carry out aseptic culture rhizoma Gastrodiae operation production process in sterile working case inside; The aseptic culture rhizoma Gastrodiae is produced used seeds of Gastrodia elata, can select the seeds of Gastrodia elata through aseptic cultivation for use, also can be chosen at the seed that cultivates under the normal condition, and the bacterium that disappears of seeds of Gastrodia elata can be implemented with medicine or other method; The fiber crops kind can be chosen rice fiber crops, the hemp that vegetative propagation goes out; Also can select for use the seeds of Gastrodia elata of cultivating by sexual selfing or hybridization to cultivate; The medium of cultivation rhizoma Gastrodiae can be chosen various types of medium according to the condition of production, the use of can arranging in pairs or groups mutually of various medium.

A, medium are the gastrodin cultivation method of loose shape: open the lid on cultivation bag, the case, the rhizoma Gastrodiae kind behind the bacterium that disappears, be planted in the loose culture base-material of bacterium material, the cultivation of rhizoma Gastrodiae also can be carried out simultaneously with the procreation of bacterium material; The medium of loose shape, armillaria mellea can be grown in medium inside, and the rhizoma Gastrodiae kind can directly utilize loose culture base-material to cultivate, the inside of rhizoma Gastrodiae cultivation at medium; The cultivation thing of rhizoma Gastrodiae also can utilize part or all to utilize materials such as soil behind the bacterium that disappears, sand, perlite to cultivate rhizoma Gastrodiae; Also configurable solids, semiliquid medium (bacterium material) carry out the energy proportioning in the medium of loose shape, improve the medium utilization ratio.

B, solid culture medium cultivation rhizoma Gastrodiae method: utilize solid culture medium (bacterium material, bacterium base) cultivation rhizoma Gastrodiae, in cultivation bag, case, can good seeds of Gastrodia elata be put into cultivation bag, case disappearing bacterium, the seed of rhizoma Gastrodiae can be cultivated with cultivation things such as aseptic sand, soil, also but part or all uses medium materials after the pulverizing instead as crops straw, bar to cultivate thing, wood chip, peanut shell, corncob or the like; Also can utilize the various medium materials of loose shape; So not only effectively reduced the confined space and labour intensity in cultivation bag, the case, and armillaria mellea prescribing adequate nutrition material is provided again.

C, semiliquid medium cultivation rhizoma Gastrodiae: the semiliquid medium that processes, armillaria mellea can be grown directly upon medium inside, so the rhizoma Gastrodiae kind can directly be planted in medium inside; The semiliquid medium also can take solid culture medium to plant the rhizoma Gastrodiae method simultaneously, the cultivation thing that seeds of Gastrodia elata utilizes medium material after the various pulverizing to be used as rhizoma Gastrodiae is cultivated rhizoma Gastrodiae, but also part or all cultivates rhizoma Gastrodiae with materials such as aseptic sand, soil, perlites to the cultivation thing of rhizoma Gastrodiae.

The cultivation management of d, rhizoma Gastrodiae: after the rhizoma Gastrodiae plantation is finished, cultivation bag, case can be transferred to culturing room and cultivate.Cultivation can be arranged on special-purpose factory building, also can be arranged on booth, the greenhouse carries out.Cultivation bag, suitably shading of case, indoorly should ventilate, rainproof, insulation, temperature should be in the control of 13---30 degree during production, air humidity is at 45---75%; Be installed in the ventilation hole on gastrodin cultivation bag, the case, can be set to single group or many groups, available pipe is connected, and to carrying out the aseptic gas input in the cultivation bag, provides the armillaria mellea demand.Gas exchange in the cultivation bag also can be utilized to be arranged on case, the bag closed hole and to finish.If rhizoma Gastrodiae when medium internal moisture and nutrition lack, can utilize pipeline, the hole of setting cultivating vegetative period; It is inner that water, nutrient input are delivered to the bacterium base, satisfies armillaria mellea growth requirement.After rhizoma Gastrodiae is through cultivation regularly, when rhizoma Gastrodiae grows maturation, can gather in the crops the commodity fiber crops in the bag.

8, liquid nutrient medium cultivation rhizoma Gastrodiae:

The cultivation of rhizoma Gastrodiae can realize by liquid nutrient solution (liquid nutrient medium), cultures armillaria mellea with liquid nutrient solution and is fit to its growth more, and the inner nutrition of medium is effectively absorbed, and manually can monitor at any time according to the growing state of armillaria mellea.Can utilize modern means of science and technology, on each growth link of rhizoma Gastrodiae, carry out intelligent management, can guarantee that armillaria mellea is in a kind of growth optimum state all the time, make rhizoma Gastrodiae production reach a kind of production technology of factory culture efficiently.

(1) setting of liquid nutrient medium inner membrance: liquid nutrient medium cultivation rhizoma Gastrodiae can be arranged in pond, case, the bag, and medium is provided with inner membrance, inner membrance requirement is set: the object that water absorbing capacity is arranged; As: materials such as fiber, chemical fibre, cloth, sand, perlite and solid culture medium materials, adventitia is set inner membrance is encapsulated, prevent the liquid outflow in the inner membrance, liquid nutrient solution and rhizoma Gastrodiae are effectively separated; Avoid rhizoma Gastrodiae contact nutrient liquid as far as possible; Be used for adventitia encapsulation object, can utilize the various objects such as water-proof function cloth, Polypropylence Sheet with holes, various mesh bags that have; As long as being provided with of adventitia requires to have small holes can prevent that again the liquid outflow is just passable, armillaria mellea can penetrate inner membrance to root by slit, the hole of adventitia setting and draw nutrient solution.During the cultivation rhizoma Gastrodiae, can spread out the armillaria mellea cyclophanes to the liquid nutrient medium inner membrance in advance, gastrodin cultivation also can carry out simultaneously with the procreation of armillaria mellea.The available sand of rhizoma Gastrodiae kind, soil, perlite and after crushed material such as solid culture medium materials cultivate.Cultivate rhizoma Gastrodiae with liquid nutrient solution, medium also can not be provided with inner membrance or adventitia, and the procreation of armillaria mellea and the cultivation of the rhizoma Gastrodiae directly interior of articles such as various medium materials after sand, soil, perlite, pulverizing are carried out; The input of medium liquid nutrient solution, can utilize water pump or water tank is set utilizes the discrepancy in elevation that the culture fluid for preparing is passed through pipeline, nutrient solution is delivered directly in medium inner membrance or the sand, fluid pipeline interts in medium inner membrance (sand) part, pipeline can be provided with aperture, by aperture nutrient solution is imported medium inside; The nutriment requirement of armillaria mellea growth is provided; Nutrient solution also can be treated to gather in the crops rhizoma Gastrodiae after rhizoma Gastrodiae is regularly cultivated maturation with manually implementing.Liquid nutrient medium cultivation rhizoma Gastrodiae can be chosen under the normal condition and carry out, and also can select cultivation and production under the aseptic condition.Liquid nutrient medium aseptic culture rhizoma Gastrodiae, used various material, apparatus, cultivation bag, case must carry out aseptic process, and each road production process of liquid nutrient medium aseptic culture rhizoma Gastrodiae, cultivation, cultivating and managing method are identical with solid culture base load training rhizoma Gastrodiae method; In order to strengthen the assorted bacterium anti-infection ability of liquid nutrient medium opposing, liquid nutrient medium is when cultivating rhizoma Gastrodiae, can utilize the bacterium medicines that disappear such as food antiseptic Ji, sterilization spirit, utilize liquid-transport pipe-line regularly to liquid nutrient medium carry out disinfection, sterilization, remove the assorted bacterium of liquid nutrient medium and prevent from infected chance to provide a good growing environment to armillaria mellea.

(2) preparation of liquid nutrient solution: needed various nutritive elements of armillaria mellea and energy must be by manually providing, the composition of medium liquid nutrient solution can have suitable chemistry examination Ji to be made into by various, also can be prepared as potato, corn steep liquor, peptone, brewer's wort, forest leachate, crops leachate, agricultural byproducts waste liquid or the like by the natural materials that composition is understood as yet.Being formulated as follows substantially of liquid nutrient solution: (a) moisture: water is composition the most basic in the medium.Water should be strict nontoxic, harmless.(b) carbon source: can select polysaccharide such as glucose, monose, sucrose, maltose and starch, pectin for use.(c) nitrogenous source: the organic nitrogen that can utilize is a lot, protein hydrolyzate, corn steep liquor, soybean cake powder, urea, dried silkworm chrysalis meal etc.; Also can utilize inorganic nitrogen.(d) vitamin: vitamin B1, vitamin h.(e) mineral salt: can add a spot of mineral salt, as phosphorus, potassium, sulphur, magnesium, calcium etc.(f), trace element: copper, molybdenum, iron, boron etc.(h) about the about 5.0---6.0 of PH.If utilize liquid nutrient solution to carry out the aseptic culture rhizoma Gastrodiae, the used various material of liquid nutrient medium must carry out aseptic process.

9, the aseptic sexual breeding of rhizoma Gastrodiae:

Cultivate the used seed of rhizoma Gastrodiae at present, the general offspring who all adopts pierre to go out, carry out the cultivation of commodity fiber crops with hemp, rice fiber crops, the shortcoming of this cultivation method is, after rhizoma Gastrodiae is cultivated through 2--3 (generation), the rhizoma Gastrodiae offspring can the harmful germ of heredity, has not only had a strong impact on the quality of rhizoma Gastrodiae, and output can be fallen under also.Also the someone utilizes sexual the breeding of rhizoma Gastrodiae to regenerate, and used method is to utilize the nascent bacterium of armillaria mellea to cultivate seeds of Gastrodia elata, and his shortcoming is just can obtain kind of fiber crops or commodity fiber crops through after the cultivation several times; Method of the present invention is in its difference, and method of the present invention is: select complete, the disease-free arrow fiber crops of external form, be used as female fiber crops and father fiber crops, and the arrow fiber crops of preferably selecting for use nature to obtain, size should equate that quantity is a plurality of.After the numb bacterium that disappears of female fiber crops, the father who chooses is handled, utilize the aseptic culture production method of rhizoma Gastrodiae to cultivate female fiber crops and father fiber crops; If obtain the rhizoma Gastrodiae selfed seed: can choose a plurality of female fiber crops and father fiber crops, be arranged on simultaneously a special use sterile sealing cultivation case, bag in carry out breeding; If select the artificial hybridization breeding for use, when treating that rhizoma Gastrodiae will be bloomed, available sack etc. are inserted in separately isolates, and manually hybridizes; Rhizoma Gastrodiae artificial hybridization breeding also can be placed on female fiber crops and father fiber crops separately in the sealing cultivation case and carry out; Before rhizoma Gastrodiae " property " was not reached maturity, the cultivation and production technology of rhizoma Gastrodiae, cultivation management method can utilize rhizoma Gastrodiae aseptic culture production technology method to implement.The aseptic sexual breeding of rhizoma Gastrodiae is arranged in a sealing cultivation case, the bag, is provided with certain space at sealing cultivation upper box part, and space segment is transparent casing, also is provided with the seal operation hole on glass box, in the hole gloves can be installed; Under aseptic condition, all are planted fiber crops and carry out management by synchronization, when treating that rhizoma Gastrodiae is bloomed, manually by being arranged on the seal operation hole, carry out the hybridization or the selfing breeding of rhizoma Gastrodiae, the seeds of Gastrodia elata that obtains is carried out the sterilization detoxification, check no bacterium after, under the aseptic condition, put into distilled water and carry out the appropriateness dilution, be planted in seed liquor on the aseptic armillaria mellea shoestring body or the cultivation thing of rhizoma Gastrodiae for seed liquor.Plant in the method for armillaria mellea inside: can select for use injection needle to draw seed liquor, be infused in respectively on the bacterium base of armillaria mellea of medium growth inside, also can be planted on the loose culture base-material; Seed liquor is planted in the method for armillaria mellea outside: seed liquor is sprayed at is used to train the cultivation thing of putting rhizoma Gastrodiae, cultivate rhizoma Gastrodiae as on: sand, soil, perlite and the loose culture base-material or directly seed liquor being sprayed on the shoestring body of the armillaria mellea of growing.The sexual cultivation of seeds of Gastrodia elata also can utilize under the conventional cultivation technical conditions and realize, cultivates the sexual seed of rhizoma Gastrodiae under the normal condition: each production process can be done simple sterilization or without sterilization processing; The handle hole that is arranged on sealing cultivation case, the bag can be set to semi-open.

Invention is provided by the following drawings:

Fig. 1 is the aseptic efficient cultivation rhizoma Gastrodiae technology of a kind of batch production that proposes according to the present invention, involved filtrated air filtration, purification special purpose machinery equipment schematic diagram.

Below in conjunction with Fig. 1 illustrate involved in the present invention to accompanying drawing in example and title and the machine works situation put down in writing:

The described content of Fig. 1 is; Be provided with opaque casing (1) in the front portion of schematic diagram, be provided with reflective object (2) at casing (1) inwall; In casing (1), also be provided with ultraviolet germicidal lamp (6); Casing (1) is provided with pipeline, this pipeline inserting is in the soup body of sealing spray tank (3), the air bacterium soup that disappears is housed in the spray tank (3), there is not the soup part on the top that spray tank (3) is inner, the inlet that also is provided with simultaneously pipeline and air compressor machine or blower fan (4) links, the pipeline inserting that blower fan (4) outlet is provided with carries out sterilization, transition at the bottom of sealing spray tank (5) bactericidal liquid that is provided with to air; Air after the sterilization is input to transparent sterile working tank inlet place by top (the no soup part) pipeline that is arranged on sealing spray tank (5); be provided with ultraviolet germicidal lamp (6) in the porch of control box; transparent operation casing is provided with handle hole (7); also be provided with input, the output transmission (8) of medium material at transparent operation box house; sliding door (10) is used for the turnover of culture base-material; when pulling (10) when outwards pulling out; can enter and protect in the door pocket (9), play a protective role.

The outstanding substantive distinguishing features that the present invention has and the remarkable technological progress that obtains:

The realization of the artificial preparation of the solid of culture medium, semisolid, loose shape, liquid has changed existing Gastrodin cultivation production technology method has improved production efficiency, and makes the cultivation of rhizoma Gastrodiae not retrained by season. The enforcement of aseptic culture rhizoma Gastrodiae can make the nutrition in the culture medium effectively utilize. The setting of semisolid culturemedium, Changed the mode that the inner nutrition of culture medium exists, be fit to close ring bacteria growing requirement, Gastrodia aseptic sexual Cultivate and inoculation method, can make the disposable cultivation of seeds of Gastrodia elata obtain commodity fiber crops and kind fiber crops; Fluid nutrient medium Arrange, manually can according to close ring bacteria growing situation, at any time adjust the supply situation of culture medium nutrition. Culture medium The realization of artificial preparation effectively utilizes farmland straw, bar, agricultural byproducts, chemical products etc., and rhizoma Gastrodiae is planted Training is produced and has been obtained sustainable development. Established simultaneously and can utilize modern technologies, machinery equipment, section's branch means To gastrodin cultivation production, grow and carry out intelligent management at links, make gastrodin cultivation production Reached a kind of efficient industrial cultivation technique.

Claims

A kind of efficient industrial cultivation rhizoma Gastrodiae technical method and production technology content that invention relates to include following:
1, culture medium preparation:

Selecting for use of medium material: available various farmlands straw, bar (as: corncob, corn stalk, careless straw, beanstalk or the like), various " shells " (as: peanut shell, cotton seed hull etc.), also available crops and agricultural byproducts are as corn flour, bean powder, wheat bran, potato, sugar or the like, also available forest, shrub, sugared slag, according to foam, weed tree sawdust, some being rich in nutrition materials such as branch, leaf all can be used as the medium material; Medium also can add the various suitable chemical elements that have;
2, the processing of medium: the various materials that medium is used, can carry out pulverization process, can not pulverize yet; Various medium materials can use separately, also can be to the material of various medium, and what be allowed a choice carries out the energy rational proportion; Can be the material that is used for medium, be formulated as multiple different prescription, dissimilar; Make it medium and reach that nutrition is complete, C/N ratio is reasonable; Can add some auxiliary materials such as gypsum, lime when the preparation medium, make the medium pH value about 5.0---6.0, humidity can be between 50---75%; After culture base-material is deployed, with artificial or machine, in the instruments such as cultivation bag, case that culture base-material is packed into;

Medium also can be according to the various different requirements of cultivation rhizoma Gastrodiae production technology, culture base-material be processed into various dissimilar as: pulverize the loose shape in back, solid, semisolid (semiliquid) or the like;

The method that a, medium are processed into solid is: prepared culture medium material (already pulverised or do not have the material pulverized), can add bonding Ji (also can not add colloid) such as colloid, carrying out extrusion processing is various bodies, by extrusion processing, the various materials of medium are condensed in together mutually, reach certain intensity, density, extruding can utilize special mechanical equipment or artificial; Processed good solid culture medium, other method such as also available various mesh bags, plastic bag with holes, paper bag is packed medium;

B, medium are processed as semi-liquid method: can select for use one or more medium materials to carry out rational proportion, make the semiliquid formula medium of number of different types; Making in the middle of the semiliquid formula medium material, can be selected into after crushed potato, pachyrhizus, agar, corn flour, bean powder, taro etc. in right amount, making the medium after its processing, can reach a kind of paste, freeze the semi-liquid stage of shape; As: the number of different types agar medium that processes with other prescriptions such as agar, potato, sugar, wood chips; Also can utilize multiple different medium material as: utilizing has suitable chemical element, formulated to go out multiple multi-form semisolid culturemedium; The semiliquid medium that processes can be packed into instruments such as cultivation bag, casees, and methods such as also available various mesh bags, paper bag are packed;

C, in order to strengthen the harmful not infected ability of bacterium of medium opposing, when the various medium of processing, can needs be formulated in the middle of the medium material easily by the material of microbial contamination and nutrient content higher material as corn flour, bean powder, potato or the like material, be formulated in the central core of medium; The setting of medium external surface layer is not easy by the material of microbial contamination or nutrient content less substance as the farmland straw of pulverizing, bar, wood chip, paper bag, soil, husky or the like; The medium internal layer is wrapped up; Medium can be effectively reduced like this by the chance of microbial contamination, the anticorrosion materials that are used for food can also be added in medium inside in order to strengthen the anti-sense ability of medium;
3, the setting of cultivation bag, the aseptic ventilation hole of case: armillaria mellea has the function of drawing oxygen in process of growth; Therefore, cultivation bag, case will be provided with aseptic vent device, are used for gas exchange in bag, the case; The various medium that process are behind the instruments such as the cultivation bag of packing into, case, at the sack or the chest sealing part (feeding mouth place) of cultivation rhizoma Gastrodiae, a ring is set, sack is nested in the ring, sack upset part outside ring, with a lid that matches, be pressed on the ring closely again; Make cultivation bag and ring, closely cooperate by " lid " formation; On lid, also be provided with, one or two ventilation holes (pipeline), in the hole, can be provided with, the gas in the sack is exchanged, the device that can prevent the harmful bacteria invasion and attack again as; Cotton, sponge, mistake bacteriological filtration paper or the like; The ventilation hole that is provided with on cultivation bag, the case also can directly seal with objects such as cotton, sponges; Needed gas in the cultivation bag, case also can pass through pipeline (ventilation hole) and directly import filtrated air behind disinfection filtering;
4, medium sterilization operation: the various types of medium that will prepare, process after installing with apparatus such as cultivation bag, casees, can enter the sterilization production process; Sterilization available dedicated equipment is carried out high temperature high pressure sterilizing as boiler, micro-wave oven, and other method such as the also available medicine of sterilization, fermentation is implemented;
5, filtrated air filtration, purification special equipment device:

After medium is finished sterilization process, can carry out the aseptic cultivation of bacterial classification of armillaria mellea and the aseptic culture production process of procreation and rhizoma Gastrodiae, production operation can be carried out at the set aseptic transparent operation case of filtrated air filtering and purifying, and this operation also can be carried out under aseptic conditions such as desinfection chamber, sterile board; Filtrated air filtration, purification production equipment special device: optional compressor or the blower fan (4) got, can be at inlet, the port of export of blower fan, be provided with seal case (3), the output of air disinfection soup and two air, input channel are housed in seal case (3), blower fan (4) is by being arranged on the pipeline in the sealing spray tank (3), can suck air or be blown into soup inside,, kill the harmful bacteria in the air the gas bacterium of filtering, purify, disappear; The sterilization of air also can be arranged on an opaque casing (1), and one or several ultraviolet sterilizing lamps (6) can be installed in casing (1) inside; In order to strengthen sterilizing ability, also can be provided with refraction reflection function good object as objects such as eyeglasses at cabinet wall to air; Make cabinet wall bright and clean (2), can increase bactericidal effect like this air; Air in the case is after sterilization, and available blower fan sucks or be blown into sterile production operational sequence (in the control box) by the pipeline that is arranged on the case; More than two kinds of technical methods to air disinfection, filtration, purification: can contact separately, be arranged in parallel, two kinds of method for disinfection also can carry out series, parallel simultaneously to be used; And can be arranged on the outlet or the arrival end of blower fan or compressor (4); By blower fan or compressor (4), the aseptic gas behind the disinfection filtering, being transported in the sterile production operational sequence or being transported to provides rhizoma Gastrodiae, armillaria mellea to use in cultivation bag, the case; The sterile production operational sequence, be arranged on a transparent seal operation casing (if use machine operation, casing can be provided with arbitrarily) in, be filled aseptic gas in the case, (at the box house of sterile working, also ultraviolet germicidal lamp can be set), be provided with seal operation hole (7) on operation casing both sides, in the hole, devices such as rubber gloves or manipulator can be installed; Handle hole also can be set to semi-open; In sterile working case production process part, also be provided with transmission device (8), be used for the output and the input of medium; The turnover of medium also can be finished by the semi-open handle hole that is provided with; In the various sterile working production processes of gastrodin cultivation as: gastrodin cultivation, armillaria mellea are cultivated, the procreation of bacterium material, and be available artificial or finish at the sterile working case with machine; At the transparent operation box back that the sterile working production process is provided with, be provided with a sealing sliding door (10) that can open, the turnover that is used to expect; When door was outwards pulled out, door can enter in the protection door pocket (9) of setting;
6, the aseptic cultivation and the procreation of armillaria mellea bacterial classification:

The aseptic cultivation of a, armillaria mellea bacterial classification: this operation can be carried out in the set sterile working production process (transparent operation casing) of filtrated air filtration, purification special equipment device, also can carry out under the aseptic conditions of work such as sterile board, superclean bench at desinfection chamber; Select the children tender, thick, healthy, armillaria mellea is had the strong part of survival ability as far as possible, have the shoestring body integral body of the old and new's armillaria mellea to take off long, the sterilization processing that carries out disinfection is killed attached to the bacterium of mixing that is harmful on the armillaria mellea; If the shoestring body is the medication liquid disinfectant, available sterile water is removed soup and is wiped the solid carbon dioxide branch; Under aseptic condition, insert manufactured, pass through sterilization process, semiliquid medium inside; As: multiple agar medium that makes with various prescriptions, type or the semiliquid medium that makes with other method; After semisolid culturemedium inserted bacterial classification, instruments such as available gastrodin cultivation bag, bottle, chest were packed into, after the aseptic sealing, are transferred to culturing room from operating room, carry out the bacterial classification aseptic culture of armillaria mellea; Culturing room requires that uniform temperature is arranged, Controllable Temperature built in about the 13-28 degree, the suitable shading of medium; Gas in cultivation bag, the case can be finished by being arranged on the sealing ventilation hole that covers, also can utilize the pipeline input to offer armillaria mellea (the aseptic cultivation of armillaria mellea bacterial classification also can be arranged on and carry out in the various cultivation apparatus cultivating under the air-proof condition) through the filtrated air of filtrated air filtering and purifying; Armillaria mellea shoestring body is in the semiliquid medium, after the certain hour cultivation, armillaria mellea will be at the inner a large amount of shoestring body that makes new advances of multiplying of medium, the aseptic armillaria mellea bacterial classification that cultivates by this method, can be used for carrying out the procreation of the bacterium material (bacterium base) of all kinds medium, also can directly be used for the cultivation and production of rhizoma Gastrodiae as the bacterium material; (the shoestring body of the armillaria mellea that cultivates with this method also can be planted the rhizoma Gastrodiae technology to existing, carries out the improvement of armillaria mellea and regenerates); The aseptic cultivation of armillaria mellea bacterial classification and procreation also can be passed through: female plant separate, female plant cultivate, original seed is cultivated the back, when nascent mycelial growth is the shoestring body, the cultivated species of practicable armillaria mellea is cultivated, after medium is multiplied through the bacterium material, cultivate the method realization that rhizoma Gastrodiae is produced again;

The bacterium material of b, armillaria mellea (bacterium base) procreation: after the aseptic breeding strain of armillaria mellea is good, can be according to the condition of production, the various medium that process are carried out the bacterium material of armillaria mellea and multiply production process, the production operation operation can be carried out under the aseptic conditions such as sterile working glass box of filtrated air filtering and purifying setting; Operate available artificial or machine enforcement; What prepare, process, various types of medium of sterilization process have been finished; As: various materials through pulverizing the prescription back in bulk in sack, chest medium or the solid that processes, semi-liquid medium or the forest that need not pulverize, shrub, straw, bar etc. are used to cultivate the material of rhizoma Gastrodiae, the various medium that need carry out the bacterium material procreation of armillaria mellea, available various apparatus or cultivation bag, case are packed into; Choose not by living contaminants, healthy, the armillaria mellea that has nutrient source (bacterium material, bacterium base), under aseptic condition, choosing to put in right amount needs the various medium of procreation to inoculate procreation, after the culture medium inoculated sealing, change bacterium base (bacterium material) procreation that culturing room carries out the medium armillaria mellea over to; The bacterium material of armillaria mellea (bacterium base) cultivating method and culture technology require identical with the aseptic breeding method of armillaria mellea bacterial classification; After medium is cultivated through certain hour,, when multiplying on the needed medium regular period, multiply the bacterium material (medium) of getting well, be can be used for cultivating rhizoma Gastrodiae production when the shoestring body of armillaria mellea; The procreation of the cultivation of armillaria mellea and bacterium material, also can be with after gathering in the crops rhizoma Gastrodiae last year, medium still have existence reasonable, not by living contaminants, healthy armillaria mellea medium, aseptic condition takes out down, be used as the cultivation and the procreation of next round armillaria mellea, also can directly be used for the cultivation and production of rhizoma Gastrodiae lower whorl as the bacterium material;
7, the aseptic culture production of rhizoma Gastrodiae:

After the bacterium base of armillaria mellea (bacterium material) procreation is good (cultivation of rhizoma Gastrodiae also can be carried out simultaneously with bacterium base, the procreation of bacterium material of armillaria mellea), gastrodin cultivation is not subjected to seasonal restriction, can carry out gastrodin cultivation production at any time according to the condition of production; The gastrodin cultivation production process, being arranged on sterile working production case carries out, cultivation rhizoma Gastrodiae needed various medium, needed various apparatus as cultivation bag, the various medium that process, bacterium material, rhizoma Gastrodiae kind, thimerosal or the like apparatus, are put into and carried out aseptic process in the sterile production control box; Under aseptic condition, manually can utilize to be arranged on that handle hole carries out sterile working production on the glass box, also can utilize the special purpose machinery apparatus, carry out aseptic culture rhizoma Gastrodiae operation production process in sterile working case inside; The aseptic culture rhizoma Gastrodiae is produced used seeds of Gastrodia elata, can select the seeds of Gastrodia elata through aseptic cultivation for use, also can be chosen at the seed that cultivates under the normal condition, and the bacterium that disappears of seeds of Gastrodia elata can be implemented with medicine or other method; The fiber crops kind can be chosen rice fiber crops, the hemp that vegetative propagation goes out; Also can select for use the seeds of Gastrodia elata of cultivating by sexual selfing or hybridization to cultivate; The medium of cultivation rhizoma Gastrodiae can be chosen various types of medium according to the condition of production, the use of can arranging in pairs or groups mutually of various medium;

A, medium are the gastrodin cultivation method of loose shape: open the lid on cultivation bag, the case, the rhizoma Gastrodiae kind behind the bacterium that disappears, be planted in the loose culture base-material of bacterium material, the cultivation of rhizoma Gastrodiae also can be carried out simultaneously with the procreation of bacterium material; The medium of loose shape, armillaria mellea can be grown in medium inside, and the rhizoma Gastrodiae kind can directly utilize loose culture base-material to cultivate, the inside of rhizoma Gastrodiae cultivation at medium; The cultivation thing of rhizoma Gastrodiae also can utilize part or all to utilize materials such as soil behind the bacterium that disappears, sand, perlite to cultivate rhizoma Gastrodiae; Also configurable solids, semiliquid medium (bacterium material) carry out the energy proportioning in the medium of loose shape, improve the medium utilization ratio;

B, solid culture medium cultivation rhizoma Gastrodiae method: utilize solid culture medium (bacterium material, bacterium base) cultivation rhizoma Gastrodiae, in cultivation bag, case, can good seeds of Gastrodia elata be put into cultivation bag, case disappearing bacterium, the seed of rhizoma Gastrodiae can be cultivated with cultivation things such as aseptic sand, soil, also but part or all uses medium materials after the pulverizing instead as crops straw, bar to cultivate thing, wood chip, peanut shell, corncob or the like; Also can utilize the various medium materials of loose shape; So not only effectively reduced the confined space and labour intensity in cultivation bag, the case, and armillaria mellea prescribing adequate nutrition material is provided again;

C, semiliquid medium cultivation rhizoma Gastrodiae: the semiliquid medium that processes, armillaria mellea can be grown directly upon medium inside, so the rhizoma Gastrodiae kind can directly be planted in medium inside; The semiliquid medium also can take solid culture medium to plant the rhizoma Gastrodiae method simultaneously, the cultivation thing that seeds of Gastrodia elata utilizes medium material after the various pulverizing to be used as rhizoma Gastrodiae is cultivated rhizoma Gastrodiae, but also part or all cultivates rhizoma Gastrodiae with materials such as aseptic sand, soil, perlites to the cultivation thing of rhizoma Gastrodiae;

The cultivation management of d, rhizoma Gastrodiae: after the rhizoma Gastrodiae plantation is finished, cultivation bag, case can be transferred to culturing room and cultivate; Cultivation can be arranged on special-purpose factory building, also can be arranged on booth, the greenhouse carries out; Cultivation bag, suitably shading of case, indoorly should ventilate, rainproof, insulation, temperature should be in the control of 13---30 degree during production, air humidity is at 45---75%; Be installed in the ventilation hole on gastrodin cultivation bag, the case, can be set to single group or many groups, available pipe is connected, and to carrying out the aseptic gas input in the cultivation bag, provides the armillaria mellea demand; Gas exchange in the cultivation bag also can be utilized to be arranged on case, the bag closed hole and to finish; If rhizoma Gastrodiae when medium internal moisture and nutrition lack, can utilize pipeline, the hole of setting cultivating vegetative period; It is inner that water, nutrient input are delivered to the bacterium base, satisfies armillaria mellea growth requirement; After rhizoma Gastrodiae is through cultivation regularly, when rhizoma Gastrodiae grows maturation, can gather in the crops the commodity fiber crops in the bag;
8, liquid nutrient medium cultivation rhizoma Gastrodiae:

The cultivation of rhizoma Gastrodiae can realize by liquid nutrient solution (liquid nutrient medium), cultures armillaria mellea with liquid nutrient solution and is fit to its growth more, and the inner nutrition of medium is effectively absorbed, and manually can monitor at any time according to the growing state of armillaria mellea; Can utilize modern means of science and technology, on each growth link of rhizoma Gastrodiae, carry out intelligent management, can guarantee that armillaria mellea is in a kind of growth optimum state all the time, make rhizoma Gastrodiae production reach a kind of production technology of factory culture efficiently;

(1) setting of liquid nutrient medium inner membrance: liquid nutrient medium cultivation rhizoma Gastrodiae can be arranged in pond, case, the bag, and medium is provided with inner membrance, inner membrance requirement is set: the object that water absorbing capacity is arranged; As: materials such as fiber, chemical fibre, cloth, sand, perlite and solid culture medium materials, adventitia is set inner membrance is encapsulated, prevent the liquid outflow in the inner membrance, liquid nutrient solution and rhizoma Gastrodiae are effectively separated; Avoid rhizoma Gastrodiae contact nutrient liquid as far as possible; Be used for adventitia encapsulation object, can utilize the various objects such as water-proof function cloth, Polypropylence Sheet with holes, various mesh bags that have; As long as being provided with of adventitia requires to have small holes can prevent that again the liquid outflow is just passable, armillaria mellea can penetrate inner membrance to root by slit, the hole of adventitia setting and draw nutrient solution; During the cultivation rhizoma Gastrodiae, can spread out the armillaria mellea cyclophanes to the liquid nutrient medium inner membrance in advance, gastrodin cultivation also can carry out simultaneously with the procreation of armillaria mellea; The available sand of rhizoma Gastrodiae kind, soil, perlite and after crushed material such as solid culture medium materials cultivate; Cultivate rhizoma Gastrodiae with liquid nutrient solution, medium also can not be provided with inner membrance or adventitia, and the procreation of armillaria mellea and the cultivation of the rhizoma Gastrodiae directly interior of articles such as various medium materials after sand, soil, perlite, pulverizing are carried out; The input of medium liquid nutrient solution, can utilize water pump or water tank is set utilizes the discrepancy in elevation that the culture fluid for preparing is passed through pipeline, nutrient solution is delivered directly in medium inner membrance or the sand, fluid pipeline interts in medium inner membrance (sand) part, pipeline can be provided with aperture, by aperture nutrient solution is imported medium inside; The nutriment requirement of armillaria mellea growth is provided; Nutrient solution also can be treated to gather in the crops rhizoma Gastrodiae after rhizoma Gastrodiae is regularly cultivated maturation with manually implementing; Liquid nutrient medium cultivation rhizoma Gastrodiae can be chosen under the normal condition and carry out, and also can select cultivation and production under the aseptic condition; Liquid nutrient medium aseptic culture rhizoma Gastrodiae, used various material, apparatus, cultivation bag, case must carry out aseptic process, and each road production process of liquid nutrient medium aseptic culture rhizoma Gastrodiae, cultivation, cultivating and managing method are identical with solid culture base load training rhizoma Gastrodiae method; In order to strengthen the assorted bacterium anti-infection ability of liquid nutrient medium opposing, liquid nutrient medium is when cultivating rhizoma Gastrodiae, can utilize the bacterium medicines that disappear such as food antiseptic Ji, sterilization spirit, utilize liquid-transport pipe-line regularly to liquid nutrient medium carry out disinfection, sterilization, the time and remove the assorted bacterium of liquid nutrient medium and prevent from infected chance to provide a good growing environment to armillaria mellea;

(2) preparation of liquid nutrient solution: needed various nutritive elements of armillaria mellea and energy must be by manually providing, the composition of medium liquid nutrient solution can have suitable chemistry examination Ji to be made into by various, also can be prepared as potato, corn steep liquor, peptone, brewer's wort, forest leachate, crops leachate, agricultural byproducts waste liquid or the like by the natural materials that composition is understood as yet; Being formulated as follows substantially of liquid nutrient solution: (a) moisture: water is composition the most basic in the medium; Water should be strict nontoxic, harmless; (b) carbon source: can select polysaccharide such as glucose, monose, sucrose, maltose and starch, pectin for use; (c) nitrogenous source: the organic nitrogen that can utilize is a lot, protein hydrolyzate, corn steep liquor, soybean cake powder, urea, dried silkworm chrysalis meal etc.; Also can utilize inorganic nitrogen; (d) vitamin: vitamin B1, vitamin h; (e) mineral salt: can add a spot of mineral salt, as phosphorus, potassium, sulphur, magnesium, calcium etc.; (f), trace element: copper, molybdenum, iron, boron etc.; (h) about the about 5.0---6.0 of PH; If utilize liquid nutrient solution to carry out the aseptic culture rhizoma Gastrodiae, the used various material of liquid nutrient medium must carry out aseptic process;
9, the aseptic sexual breeding of rhizoma Gastrodiae:

Select complete, the disease-free arrow fiber crops of external form, be used as female fiber crops and father fiber crops, the arrow fiber crops of preferably selecting for use nature to obtain, size should equate that quantity is a plurality of; After the numb bacterium that disappears of female fiber crops, the father who chooses is handled, utilize the aseptic culture production method of rhizoma Gastrodiae to carry out; If obtain the rhizoma Gastrodiae selfed seed: can choose a plurality of female fiber crops and father fiber crops, be arranged on simultaneously a special use sterile sealing cultivation case, bag in carry out breeding; If select the artificial hybridization breeding for use, treat with sack rhizoma Gastrodiae to be inserted in separately when rhizoma Gastrodiae will be bloomed and carry out artificial hybridization; The cultivation of rhizoma Gastrodiae artificial hybridization breeding also can be placed on female fiber crops and father fiber crops separately in the cultivation case and carry out the artificial hybridization breeding; Before rhizoma Gastrodiae " property " was not reached maturity, the cultivation and production technology of rhizoma Gastrodiae, cultivation management method can utilize rhizoma Gastrodiae aseptic culture production technology method to implement; The aseptic sexual breeding of rhizoma Gastrodiae is arranged in a sealing cultivation case, the bag, is provided with certain space at sealing cultivation upper box part, and space segment is transparent casing, also is provided with the seal operation hole on glass box, in the hole gloves can be installed; Under aseptic condition, all are planted fiber crops and carry out management by synchronization, when treating that rhizoma Gastrodiae is bloomed, manually by being arranged on the seal operation hole, carry out the hybridization or the selfing of rhizoma Gastrodiae, the seeds of Gastrodia elata that obtains is carried out the sterilization detoxification, check no bacterium after, under the aseptic condition, put into distilled water and carry out the appropriateness dilution, be planted in seed liquor on the aseptic armillaria mellea shoestring body or the cultivation thing of rhizoma Gastrodiae for seed liquor; Plant in the method for armillaria mellea inside: can select for use injection needle to draw seed liquor, be infused in respectively on the bacterium base of armillaria mellea of medium growth inside, also can be planted on the loose culture base-material; Seed liquor is planted in the method for armillaria mellea outside: seed liquor is sprayed at is used to train the cultivation thing of putting rhizoma Gastrodiae, cultivate rhizoma Gastrodiae as on: sand, perlite and the loose culture base-material or directly seed liquor being sprayed on the shoestring body of the armillaria mellea of growing; The sexual cultivation of seeds of Gastrodia elata also can utilize under the conventional cultivation technical conditions and realize, cultivates rhizoma Gastrodiae mictic seed under the normal condition, and each production process can be done simple sterilization or without sterilization processing; The handle hole that is arranged on sealing cultivation case, the bag can be set to semi-open.