CN109197380A

CN109197380A - A kind of planting technology of novel edible bacterium

Info

Publication number: CN109197380A
Application number: CN201811391126.0A
Authority: CN
Inventors: 甘铃
Original assignee: Zunyi State Sowing Ring Jingwei Letinous Edodes Planting Co Ltd
Current assignee: Zunyi State Sowing Ring Jingwei Letinous Edodes Planting Co Ltd
Priority date: 2018-11-19
Filing date: 2018-11-19
Publication date: 2019-01-15

Abstract

The invention discloses a kind of planting technology of novel edible bacterium, the implantation methods are the following steps are included: collect hypha of edible fungus clast, in the first PDA culture medium of access；Culture obtains the first mycelium set；Thiazolyl blue and crystal violet mixed solution are instilled into the first mycelium set, and is supplemented the nutrients, and culture obtains mycelium；Lywallzyme is added into mycelium, until after enzymatic hydrolysis is complete, isolated cultivation body；Body will be cultivated to access in the second culture medium, culture obtains mature body.The present invention can obtain edible mushroom using the culture of mycelia clast, and contain the Effective selection to culture body in this process, and the edible mushroom made is high-quality, in good taste.

Description

A kind of planting technology of novel edible bacterium

Technical field

The present invention relates to the technical field of edible mushroom culture, in particular to a kind of planting technologies of novel edible bacterium.

Background technique

For edible mushroom because its taste is fresh and tender, nutritive value is high, more to the advantageous active constituent of human body, and more and more by Consumer's likes that often supply falls short of demand.The implantation methods of edible mushroom generally comprise spore plantation and tissue cultures etc., wherein spore Son plantation can be carried out by directly collecting edible mushroom spore, and technical difficulty is relatively low, but one side edible mushroom spore volume is small, The speed of growth is slow, and screening difficulty is big, causes the economic cost directly planted using spore higher, the period is long, and existing tissue Culture mostly uses mycelia, fructification etc., and cost of material is high, and output capacity is low, and screening capacity is poor in incubation, cause to spend compared with Still there is more low quality individual in the edible mushroom maturation body that more costs obtain.

Summary of the invention

It is an object of the invention to propose that one kind can be by be used as waste material or reworked material to utilize during planting edible mushroom The method that mycelia clast culture obtains edible mushroom, and the Effective selection to culture body, the food made are contained in this process It is high-quality with bacterium, it is in good taste.

Technical scheme is as follows:

A kind of planting technology of novel edible bacterium comprising following steps:

(1) hypha of edible fungus clast is collected, is accessed in the first PDA culture medium；

(2) the first PDA culture medium after access is cultivated 5~10 days at 20~25 DEG C, growth obtains the in culture medium One mycelium set；

(3) to obtaining instilling thiazolyl blue and crystal violet mixed solution in the first PDA culture medium of the first mycelium set, and It supplements the nutrients into the first PDA culture medium, it is long into the PDA culture medium to continue culture at 20~25 DEG C for shaken well Until full mycelium, gained mycelium is filtered thereafter；

(4) lywallzyme is added in the mycelium being obtained by filtration to step (3), until distillation is added thereto after enzymatic hydrolysis is complete Water is centrifugated thereafter, collects seabed sediment matter, obtains cultivating body after washing；

(5) the cultivation body is accessed in the second culture medium, constant temperature incubation, and carries out daily management, until being adopted after its maturation Receipts.

Preferably: the edible mushroom is selected from coprinus comatus, Pleurotus eryngii, needle mushroom, Hericium erinaceus, mushroom, straw mushroom, beef liver One of bacterium, agrocybe, seafood mushroom are a variety of.

It is also preferred that: first PDA culture medium is fluid nutrient medium comprising following components: potato, grape Sugar, agar, peptone, inorganic salts, mushroom extract.

It is it is further preferred that the inorganic salts include one of magnesium sulfate, calcium sulfate, potassium dihydrogen phosphate or more Kind.

It is also preferred that: the concentration of thiazolyl blue is 1~10mg/L, crystallization in the thiazolyl blue and crystal violet mixed solution Purple concentration is 5~15mg/L.

It is also preferred that: the additional amount of the thiazolyl blue and crystal violet mixed solution is the first PDA culture medium matter The 5~10% of amount.

It is also preferred that: the nutritional ingredient supplemented is selected from glucose, fructose, starch, cellulose, albumen powder, nothing One of machine salt, mushroom extract are a variety of.

It is also preferred that: second culture medium includes smashed cotton seed hulls, wheat bran, lime, gypsum, corncob, Agar, peptone, potato and water.

The present invention can be eaten the mycelia clast culture for being used as waste material or reworked material to utilize during planting edible mushroom Bacterium is grown in the first PDA culture medium by mycelia clast first during culture and obtains basic mycelia, thereafter will be basic Mycelia is screened by irritation inducer thiazolyl blue and crystal violet, then obtains excellent protoplast through enzymatic hydrolysis, thereafter will The protoplast that this is excellent, growth ability is strong is cultivated in the second culture medium and obtains the edible mushroom of high quality.

The present invention can be eaten by the mycelia clast culture for being used as waste material or reworked material to utilize during planting edible mushroom With bacterium, and the Effective selection to culture body is contained in this process, the edible mushroom made is high-quality, in good taste.

Specific embodiment

(5) the cultivation body is accessed in the second culture medium, constant temperature incubation, and carries out daily management, until being adopted after its maturation Receipts.Wherein:

The edible mushroom can be coprinus comatus, Pleurotus eryngii, needle mushroom, Hericium erinaceus, mushroom, straw mushroom, bolete, agrocybe, sea One of fresh mushroom is a variety of.

First PDA culture medium is fluid nutrient medium, including following components: potato, glucose, agar, peptone, Inorganic salts, mushroom extract, wherein mushroom extract is that mushroom is boiled the liquid that 20~30min is obtained using deionized water, Its additional amount is the 1/15~1/5 of the PDA culture medium gross mass, and inorganic salts preferably include magnesium sulfate, calcium sulfate and biphosphate Potassium can also further comprise the microelement that other edible mushrooms need.

The concentration of thiazolyl blue is 1~10mg/L in the thiazolyl blue and crystal violet mixed solution, the concentration of crystal violet is 5~ The additional amount of 15mg/L, the thiazolyl blue and crystal violet mixed solution is the 5~10% of the first PDA culture medium quality, herein The first described PDA culture medium is the first initial PDA culture medium, that is, does not have started the PDA culture for carrying out the culture of mycelia clast Base.

The nutritional ingredient supplemented is to extract selected from glucose, fructose, starch, cellulose, albumen powder, inorganic salts, mushroom One of liquid is a variety of, preferably contains above-mentioned nutritional ingredient simultaneously.

Second culture medium includes smashed cotton seed hulls, wheat bran, lime, gypsum, corncob, agar, peptone, horse Bell potato and water.

Claims

1. a kind of planting technology of novel edible bacterium, it is characterised in that: the implantation methods the following steps are included:

(2) the first PDA culture medium after access is cultivated 5~10 days at 20~25 DEG C, growth obtains the first bacterium in culture medium Filament set；

(3) to obtaining instilling thiazolyl blue and crystal violet mixed solution in the first PDA culture medium of the first mycelium set, and to the It supplements the nutrients in one PDA culture medium, shaken well continues culture at 20~25 DEG C and covers with bacterium into the PDA culture medium Until filament, gained mycelium is filtered thereafter；

(4) lywallzyme is added in the mycelium being obtained by filtration to step (3), until distilled water is added thereto after enzymatic hydrolysis is complete, After be centrifugated, collect seabed sediment matter, obtained after washing cultivate body；

(5) the cultivation body is accessed in the second culture medium, constant temperature incubation, and carries out daily management, until harvesting is after its maturation It can.

2. a kind of planting technology of novel edible bacterium according to claim 1, it is characterised in that: the edible mushroom be selected from One of coprinus comatus, Pleurotus eryngii, needle mushroom, Hericium erinaceus, mushroom, straw mushroom, bolete, agrocybe, seafood mushroom are a variety of.

3. a kind of planting technology of novel edible bacterium according to claim 1, it is characterised in that: the first PDA culture Base is fluid nutrient medium comprising following components: potato, glucose, agar, peptone, inorganic salts, mushroom extract.

4. a kind of planting technology of novel edible bacterium according to claim 3, it is characterised in that: the inorganic salts include sulphur One of sour magnesium, calcium sulfate, potassium dihydrogen phosphate are a variety of.

5. a kind of planting technology of novel edible bacterium according to claim 1, it is characterised in that: the thiazolyl blue and crystallization The concentration of thiazolyl blue is 1~10mg/L in purple mixed solution, and the concentration of crystal violet is 5~15mg/L.

6. a kind of planting technology of novel edible bacterium according to claim 1, it is characterised in that: the thiazolyl blue and crystallization The additional amount of purple mixed solution is the 5~10% of the first PDA culture medium quality.

7. a kind of planting technology of novel edible bacterium according to claim 1, it is characterised in that: the nutritional ingredient supplemented For selected from one of glucose, fructose, starch, cellulose, albumen powder, inorganic salts, mushroom extract or a variety of.

8. a kind of planting technology of novel edible bacterium according to claim 1, it is characterised in that: the second culture medium packet Include smashed cotton seed hulls, wheat bran, lime, gypsum, corncob, agar, peptone, potato and water.