CN101463325A - Industrialized cultivation method for north aweto - Google Patents
Industrialized cultivation method for north aweto Download PDFInfo
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- CN101463325A CN101463325A CNA2008101636019A CN200810163601A CN101463325A CN 101463325 A CN101463325 A CN 101463325A CN A2008101636019 A CNA2008101636019 A CN A2008101636019A CN 200810163601 A CN200810163601 A CN 200810163601A CN 101463325 A CN101463325 A CN 101463325A
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- cordyceps militaris
- potato
- potato dextrose
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Abstract
The invention discloses a corayceps militaris industrial cultivation method. The corayceps militaris is cultivated in an industrial way by strain preparation, blending, bottling, sterilizing, inoculation, culture, germination and collection; fimilar artificial substitute materials are used as the culture materials, which reduce manufacture cost. In addition, the corayceps militaris obtained by the invention has the characteristics of good quality stability and high biological percent conversion, etc.
Description
Technical field
The invention belongs to the Edible Fungi field, specifically be meant the industrial planting method of Cordyceps militaris (L.) Link..
Background technology
Cordyceps militaris (L.) Link. is grown in Zhongshan District or shallow mountain area, can be parasitic on the pupa of various insects, requirement for growing environment is lower, so Cordyceps militaris (L.) Link. also is Cordyceps militaris (L.) Link. or pupa grass, and wild Cordyceps militaris (L.) Link. mainly is distributed in provinces such as China Jilin, Hebei, Shaanxi at present.Its main chemical ingredients pharmacology, drug effect are very similar to wild cordyceps, detection through authoritative departments such as Chinese Academy of Medical Sciences drug research analyzer room, Shenyang Branch of Chinese Academy of Sciences physical and chemical testing centers shows that main nutrition of some of Cordyceps militaris and pharmaceutical compositions such as cordycepin, Cordyceps polysaccharide, protein etc. are higher than Cordyceps sinensis.
Along with constantly providing of people's quality of the life, demand to Cordyceps militaris (L.) Link. is also increasing, natural wild Cordyceps militaris (L.) Link. has been difficult to satisfy people's demand, in recent years, manually successfully cultivate the mature sporophore of Cordyceps militaris (L.) Link., opened up an effective way for solving the Cordyceps militaris (L.) Link. resource scarcity.Provenance is degenerated, " white face " phenomenon but also exist in the artificial growth cultivation of Cordyceps militaris (L.) Link.; i.e. not annesl or over growth of hyphae and can not cause the phenomenon of grass of distinctive mycelia in cordyceps militaris cultivation; the deformity sporophore produces factor such as more and causes its output lower, has seriously restricted Cordyceps militaris (L.) Link. large-scale planting and popularization.Artificial culture Cordyceps militaris (L.) Link. in addition; its " worm " combines with " bacterium "; the most important condition is that a large amount of worm sources must be arranged; though utilize silkworm pupa, castor-oil plant silkworm chrysalis, Philosamia cynthia pupa many for host's artificial culture Cordyceps militaris (L.) Link. achieving success example; yet cost is too high; cultivated material is subject to seasonal restrictions; and present Cordyceps militaris is in the artificial culture process; its biological transformation ratio is low, bacterial classification is degenerated soon, over growth of hyphae problem such as annesl and living contaminants not; make and Cordyceps militaris output and quality instability are difficult to form large-scale production.
Summary of the invention
The objective of the invention is in order to overcome the shortcoming and defect that prior art exists, and the industrial planting method that a kind of cost is lower, produce easy, biotransformation rate height, stay-in-grade Cordyceps militaris (L.) Link. is provided.
For achieving the above object, the technical solution of the utility model is may further comprise the steps:
(1), strain preparation: preparation potato dextrose agar and improvement potato dextrose agar culture material are standby;
1. female the kind prepares: carry out separate tissue from wild Cordyceps militaris (L.) Link. and obtain female the kind, female plant by 1:20～1:25 ratio through behind the tube, 18 ℃-23 ℃ cultivations 13～15 days down, it is standby preferably to select growing way;
2. original seed preparation: potato dextrose agar or improvement potato dextrose agar are evenly packed in the culturing bottle, the charge amount of culturing bottle is 20%-40%, seal with tampon, 1h sterilizes under 0.1Mpa pressure, after the cooling, after following standby mother kind of aseptic condition pressed in the 1:1 access culturing bottle, left standstill 24h, be placed on and be cultured to a large amount of bacterium balls of appearance on the shaking table, 4 ℃ of preservations are standby in the placement refrigerator;
3. cultivar preparation: potato dextrose agar or improvement potato dextrose agar are put into fermentor tank, to substratum and the fermentor tank pipeline 1h that under 0.1Mpa pressure, sterilizes respectively, be cooled to 20 ℃, under aseptic condition, insert standby original seed, cultivate 30-36h down at 20 degrees centigrade, to fermentor tank, a large amount of bacterium balls occur;
(2), batching bottling: make culture material, and culture material evenly packed into cultivate, the charge amount of culturing bottle is 20%-40%, seals with tampon;
(3), sterilization: culturing bottle is put into Autoclave, and 1.5h-2h sterilizes under 0.11Mpa-0.15Mpa pressure;
(4), inoculation: culture material is cooled to inoculate under aseptic condition below 24 ℃, and every female kind connects 1 bottle of original seed, and every 300ml original seed connects the 10L cultivar;
(5), cultivate: will inoculate good Cordyceps militaris (L.) Link. culturing bottle move on to send out a bacterium chamber and leave standstill cultivation 24h after, place and carry out shaking culture on the shaking table, 140-160r/min, cultivated 4-6 days by 22-25 ℃, and then indoor cultivation 40 days-60 days, wherein send out the bacterium chambers temp and require 22 ℃～25 ℃, relative air humidity 65%～70%, and keep shading, and door window carries out ventilation in good time, and it is fresh to keep sending out bacterium chamber air;
(6), going out grass gathers.
As further improving and replenishing to technique scheme, the present invention can also have following further technology contents, the proportioning of the potato dextrose agar of described step (1), every 1000ml: potato 200g, glucose 20g, agar 18～20g, its preparation method is put and is added water 1000ml in the container for removing the peel potato slices, boils 15min, filter, add glucose and agar, supply water to 1000ml, heating for dissolving; After the agar dissolving, pack in the test tube with dispenser, loading amount be test tube long 1/5, tampon is put in the Autoclave beyond the Great Wall, the 0.5h that under 0.1Mpa pressure, sterilizes, pound naturally falls; Test tube is emitted on the skewback while hot cools off.
This is provided with the proportioning of the improvement potato dextrose agar that can also be described step (1), every 1000ml: potato 200g, glucose 20g, potassium primary phosphate 2g, sal epsom 1.5g, agar 18～20g, its preparation method is put and is added water 1000ml in the container for removing the peel potato slices, boil 15min, filter, add glucose and agar, supply water to 1000ml, heating for dissolving; After the agar dissolving, pack in the test tube with dispenser, loading amount be test tube long 1/5, tampon is put in the Autoclave beyond the Great Wall, the 0.5h that under 0.1Mpa pressure, sterilizes, pound naturally falls; Test tube is emitted on the skewback while hot cools off.
Further setting is that the proportioning of the culture material of described step (2) is: Semen Maydis powder 2%, glucose 2%, peptone 1%, yeast powder 0.5%, KH
2PO
40.1%, MgSO
40.05%, its pH value is 6.5.This is provided with can also be that the proportioning of culture material of described step (2) is: potato 20% Semen Maydis powder 3%, glucose 2%, peptone 0.3%,, KH
2PO
40.15%, MgSO4 0.05%, and its pH value is 6.5.
The invention has the advantages that: the present invention adopts common artificial generation material to carry out factory culture, and production cost is low, produces easyly, and the Cordyceps militaris quality stability of the present invention's acquisition is good, the biological transformation ratio advantages of higher.
Below by embodiment the present invention is done further introduction.
Embodiment
Below by embodiment the present invention is carried out concrete description; only be used for the present invention is further specified; can not be interpreted as the qualification to protection domain of the present invention, the slip-stick artist in this field can make some nonessential improvement and adjustment to the present invention according to the content of foregoing invention.
Present embodiment may further comprise the steps:
(1), strain preparation: preparation potato dextrose agar and improvement potato dextrose agar culture material are standby;
1. female the kind prepares: carry out separate tissue from wild Cordyceps militaris (L.) Link. and obtain female the kind, female plant by 1:20～1:25 ratio through behind the tube, 18 ℃-23 ℃ cultivations 13～15 days down, it is standby preferably to select growing way;
2. original seed preparation: potato dextrose agar or improvement potato dextrose agar are evenly packed in the culturing bottle, the charge amount of culturing bottle is 20%-40%, seal with tampon, 1h sterilizes under 0.1Mpa pressure, after the cooling, after following standby mother kind of aseptic condition pressed in the 1:1 access culturing bottle, left standstill 24h, be placed on and be cultured to a large amount of bacterium balls of appearance on the shaking table, 4 ℃ of preservations are standby in the placement refrigerator;
3. cultivar preparation: potato dextrose agar or improvement potato dextrose agar are put into fermentor tank, to substratum and the fermentor tank pipeline 1h that under 0.1Mpa pressure, sterilizes respectively, be cooled to 20 ℃, under aseptic condition, insert standby original seed, cultivate 30-36h down at 20 degrees centigrade, to fermentor tank, a large amount of bacterium balls occur;
Present embodiment can adopt potato dextrose agar as substratum, its proportioning, and every 1000ml:
Potato 200g, glucose 20g, agar 18～20g, its preparation method is put and is added water 1000ml in the container for remove the peel potato slices, boils 15min, filters, and adding glucose and agar are supplied water to 1000ml, heating for dissolving; After the agar dissolving, pack in the test tube with dispenser, loading amount be test tube long 1/5, tampon is put in the Autoclave beyond the Great Wall, the 0.5h that under 0.1Mpa pressure, sterilizes, pound naturally falls; Test tube is emitted on the skewback while hot cools off.
The present invention can also adopt the improvement potato dextrose agar as substratum, its composition proportion, and every 1000ml:
Potato 200g, glucose 20g, potassium primary phosphate 2g, sal epsom 1.5g, agar 18～20g,
Its preparation method is put and is added water 1000ml in the container for remove the peel potato slices, boils 15min, filters, and adding glucose and agar are supplied water to 1000ml, heating for dissolving; After the agar dissolving, pack in the test tube with dispenser, loading amount be test tube long 1/5, tampon is put in the Autoclave beyond the Great Wall, the 0.5h that under 0.1Mpa pressure, sterilizes, pound naturally falls; Test tube is emitted on the skewback while hot cools off.
(2), batching bottling: culture material evenly packed into cultivate, the charge amount of culturing bottle is 20%-40%, seals with tampon; The proportion optimization of the described culture material of present embodiment is: Semen Maydis powder 2%, glucose 2%, peptone 1%, yeast powder 0.5%, KH
2PO
40.1%, MgSO
40.05%, its pH value is 6.5.Culture material of the present invention can also adopt other proportionings to be: Semen Maydis powder 2%, glucose 2%, peptone 1%, yeast powder 0.5%, KH2PO4 0.1%, MgSO4 0.05%, its pH value are 6.5.This is provided with can also be that the proportioning of culture material of described step (2) is: potato 20% Semen Maydis powder 3%, glucose 2%, peptone 0.3%,, KH2PO4 0.15%, MgSO4 0.05%, its pH value is 6.5.
(3), sterilization: culturing bottle is put into Autoclave, and 1.5h-2h sterilizes under 0.11Mpa-0.15Mpa pressure; Present embodiment adopts autoclaved method to shorten the time of sterilization, and the present invention also can adopt the method for normal-pressure sterilization certainly, but normal-pressure sterilization need be sterilized 10 hours at least.
(4), inoculation: culture material is cooled to inoculate under aseptic condition below 24 ℃, and every female kind connects 1 bottle of original seed, and every 300ml original seed connects the 10L cultivar;
(5), cultivate: will inoculate good Cordyceps militaris (L.) Link. culturing bottle move on to send out a bacterium chamber and leave standstill cultivation 24h after, place and carry out shaking culture on the shaking table, 140-160r/min, cultivated 4-6 days by 22-25 ℃, and then indoor cultivation 40 days-60 days, wherein send out the bacterium chambers temp and require 22 ℃～25 ℃, relative air humidity 65%～70%, and keep shading, and door window carries out ventilation in good time, and it is fresh to keep sending out bacterium chamber air;
(6), going out grass gathers.
The present invention adopts common artificial generation material to carry out factory culture, and production cost is low, produces easyly, and the Cordyceps militaris quality stability of the present invention's acquisition is good, the biological transformation ratio advantages of higher.
Claims (5)
1. the industrial planting method of a Cordyceps militaris (L.) Link. is characterized in that may further comprise the steps:
(1), strain preparation: preparation potato dextrose agar and improvement potato dextrose agar culture material are standby;
1. female the kind prepares: carry out separate tissue from wild Cordyceps militaris (L.) Link. and obtain female the kind, female plant by 1:20～1:25 ratio through behind the tube, 18 ℃-23 ℃ cultivations 13～15 days down, it is standby preferably to select growing way;
2. original seed preparation: potato dextrose agar or improvement potato dextrose agar are evenly packed in the culturing bottle, the charge amount of culturing bottle is 20%-40%, seal with tampon, 1h sterilizes under 0.1Mpa pressure, after the cooling, after following standby mother kind of aseptic condition pressed in the 1:1 access culturing bottle, left standstill 24h, be placed on and be cultured to a large amount of bacterium balls of appearance on the shaking table, 4 ℃ of preservations are standby in the placement refrigerator;
3. cultivar preparation: potato dextrose agar or improvement potato dextrose agar are put into fermentor tank, to substratum and the fermentor tank pipeline 1h that under 0.1Mpa pressure, sterilizes respectively, be cooled to 20 ℃, under aseptic condition, insert standby original seed, cultivate 30-36h down at 20 degrees centigrade, to fermentor tank, a large amount of bacterium balls occur;
(2), batching bottling: make culture material, and culture material is evenly packed in the culturing bottle, the charge amount of culturing bottle is 20%-40%, seals with tampon;
(3), sterilization: culturing bottle is put into Autoclave, and 1.5h-2h sterilizes under 0.11Mpa-0.15Mpa pressure;
(4), inoculation: culture material is cooled to inoculate under aseptic condition below 24 ℃, and every female kind connects 1 bottle of original seed, and every 300ml original seed connects the 10L cultivar;
(5), cultivate: will inoculate good Cordyceps militaris (L.) Link. culturing bottle move on to send out a bacterium chamber and leave standstill cultivation 24h after, place and carry out shaking culture on the shaking table, 140-160r/min, cultivated 4-6 days by 22-25 ℃, and then indoor cultivation 40 days-60 days, wherein send out the bacterium chambers temp and require 22 ℃～25 ℃, relative air humidity 65%～70%, and keep shading, and door window carries out ventilation in good time, and it is fresh to keep sending out bacterium chamber air;
(6), going out grass gathers.
2. the industrial planting method of Cordyceps militaris (L.) Link. according to claim 1, it is characterized in that: the proportioning of the potato dextrose agar of described step (1), every 1000ml: potato 200g, glucose 20g, agar 18～20g, its preparation method is put and is added water 1000ml in the container for removing the peel potato slices, boil 15min, filter, add glucose and agar, supply water to 1000ml, heating for dissolving; After the agar dissolving, pack in the test tube with dispenser, loading amount be test tube long 1/5, tampon is put in the Autoclave beyond the Great Wall, the 0.5h that under 0.1Mpa pressure, sterilizes, pound naturally falls; Test tube is emitted on the skewback while hot cools off.
3. the industrial planting method of Cordyceps militaris (L.) Link. according to claim 1, it is characterized in that: the proportioning of the improvement potato dextrose agar of described step (1), every 1000ml: potato 200g, glucose 20g, potassium primary phosphate 2g, sal epsom 1.5g, agar 18～20g, its preparation method is put and is added water 1000ml in the container for removing the peel potato slices, boils 15min, filter, add glucose and agar, supply water to 1000ml, heating for dissolving; After the agar dissolving, pack in the test tube with dispenser, loading amount be test tube long 1/5, tampon is put in the Autoclave beyond the Great Wall, the 0.5h that under 0.1Mpa pressure, sterilizes, pound naturally falls; Test tube is emitted on the skewback while hot cools off.
4. according to the industrial planting method of claim 1 or 2 or 3 described Cordyceps militaris (L.) Link., it is characterized in that: the proportioning of the culture material of described step (2) is: Semen Maydis powder 2%, glucose 2%, peptone 1%, yeast powder 0.5%, KH
2PO
40.1%, MgSO
40.05%, its pH value is 6.5.
5. according to the industrial planting method of claim 1 or 2 or 3 described Cordyceps militaris (L.) Link., it is characterized in that: the proportioning of the culture material of described step (2) is: potato 20% Semen Maydis powder 3%, glucose 2%, peptone 0.3%,, KH
2PO
40.15%, MgSO
40.05%, its pH value is 6.5.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008101636019A CN101463325A (en) | 2008-12-16 | 2008-12-16 | Industrialized cultivation method for north aweto |
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|---|---|---|---|
| CNA2008101636019A CN101463325A (en) | 2008-12-16 | 2008-12-16 | Industrialized cultivation method for north aweto |
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ID=40804116
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Cited By (16)
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|---|---|---|---|---|
| CN101971764A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating cordyceps militaris |
| CN102440141A (en) * | 2010-10-08 | 2012-05-09 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN102511313A (en) * | 2011-12-29 | 2012-06-27 | 重庆沙科农业开发有限公司 | Cordyceps militaris cultivating greenhouse and Cordyceps militaris cultivating method |
| CN102550300A (en) * | 2012-03-10 | 2012-07-11 | 何寒 | Method for cultivating cordyceps sinensis by taking unhusked rice as culture medium |
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| CN102696402A (en) * | 2012-07-04 | 2012-10-03 | 黑龙江大学 | Culturing method of liquorice-based cordyceps militaris |
| CN102726216A (en) * | 2012-07-17 | 2012-10-17 | 杨毅 | Method for culturing cordyceps militaris anti-degradation strain |
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| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
| CN103843583A (en) * | 2014-03-06 | 2014-06-11 | 刘彦君 | Industrialized production method of green cordyceps militaris |
| CN104584855A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | Cordyceps militaris extruding, puffing and sterilizing production method |
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| CN104641944A (en) * | 2015-03-19 | 2015-05-27 | 黄秀英 | Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium |
| CN105660187A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Aseptic silkworm cordyceps militaris strain inoculation system and method |
| CN110250103A (en) * | 2019-07-29 | 2019-09-20 | 李秀丽 | A kind of method for breeding of cordyceps sinensis laying hen |
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2008
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| WO2011116649A1 (en) * | 2010-03-22 | 2011-09-29 | 江苏学府生物工程有限公司 | Culturing method for cordyceps militaris |
| CN101971764A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating cordyceps militaris |
| CN102440141A (en) * | 2010-10-08 | 2012-05-09 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN102440141B (en) * | 2010-10-08 | 2012-12-26 | 镇江市食用菌研究所 | Method for separating Cordyceps militaris strains |
| CN102511313A (en) * | 2011-12-29 | 2012-06-27 | 重庆沙科农业开发有限公司 | Cordyceps militaris cultivating greenhouse and Cordyceps militaris cultivating method |
| CN102550300A (en) * | 2012-03-10 | 2012-07-11 | 何寒 | Method for cultivating cordyceps sinensis by taking unhusked rice as culture medium |
| CN102630486A (en) * | 2012-04-16 | 2012-08-15 | 何寒 | Method for cultivating worm grass in culture medium by solidifying nutrient solution by using water-retaining agent |
| CN102696402A (en) * | 2012-07-04 | 2012-10-03 | 黑龙江大学 | Culturing method of liquorice-based cordyceps militaris |
| CN102726216A (en) * | 2012-07-17 | 2012-10-17 | 杨毅 | Method for culturing cordyceps militaris anti-degradation strain |
| CN102936609B (en) * | 2012-11-05 | 2014-12-17 | 南京农业大学 | Preparation method of swift moth paecilomyces varioti extracellular acid glycoprotein |
| CN102936609A (en) * | 2012-11-05 | 2013-02-20 | 南京农业大学 | Preparation method of swift moth paecilomyces varioti extracellular acid glycoprotein |
| CN103340396A (en) * | 2013-07-25 | 2013-10-09 | 孟凡格 | Method for producing edible and medicinal fungus powder |
| CN103340396B (en) * | 2013-07-25 | 2014-08-27 | 孟凡格 | Method for producing edible and medicinal fungus powder |
| CN104584855A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | Cordyceps militaris extruding, puffing and sterilizing production method |
| CN103733875A (en) * | 2013-11-08 | 2014-04-23 | 吉林省农业科学院 | Cordyceps militaris factory production method and process |
| CN103843583A (en) * | 2014-03-06 | 2014-06-11 | 刘彦君 | Industrialized production method of green cordyceps militaris |
| CN103843583B (en) * | 2014-03-06 | 2016-06-29 | 刘彦君 | Green Cordyceps militaris industrialization production method |
| CN104585510A (en) * | 2015-02-13 | 2015-05-06 | 秦皇岛高通生物科技有限公司 | Feed additive containing mixed bacterium fermented product and preparation method for feed additive |
| CN104585510B (en) * | 2015-02-13 | 2017-11-24 | 秦皇岛高通生物科技有限公司 | A kind of mixed fungus fermentation thing feed addictive and preparation method thereof |
| CN104641944A (en) * | 2015-03-19 | 2015-05-27 | 黄秀英 | Method for culturing cordyceps sinensis by taking unhulled wheat as culture medium |
| CN105660187A (en) * | 2016-02-23 | 2016-06-15 | 张家港顺泰元生物科技有限公司 | Aseptic silkworm cordyceps militaris strain inoculation system and method |
| CN110250103A (en) * | 2019-07-29 | 2019-09-20 | 李秀丽 | A kind of method for breeding of cordyceps sinensis laying hen |
| CN110250103B (en) * | 2019-07-29 | 2021-08-24 | 李秀丽 | Method for raising cordyceps sinensis laying hens |
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