CN104911096A - Edible fungus liquid fermentation primary strain culture and sterile inoculation device and technique - Google Patents

Edible fungus liquid fermentation primary strain culture and sterile inoculation device and technique Download PDF

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Publication number
CN104911096A
CN104911096A CN201510313920.3A CN201510313920A CN104911096A CN 104911096 A CN104911096 A CN 104911096A CN 201510313920 A CN201510313920 A CN 201510313920A CN 104911096 A CN104911096 A CN 104911096A
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tank body
inoculation
control valve
pipeline
tank
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CN201510313920.3A
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CN104911096B (en
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刘世玲
田少平
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TIANJIN WULIN ZHONGDI AGRICULTURAL TECHNOLOGY Co Ltd
Hubei Wulin Land Agricultural Technology Co Ltd
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TIANJIN WULIN ZHONGDI AGRICULTURAL TECHNOLOGY Co Ltd
Hubei Wulin Land Agricultural Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
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  • Biochemistry (AREA)
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  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention provides an edible fungus liquid fermentation primary strain culture and sterile inoculation device which comprises a tank body and a fermentation tank, wherein the top of the tank body is provided with a feed port; the feed port is provided with a feed port cover; the top of the tank body is also provided with an air filter; the air filter is provided with a compressed air inlet pipe; a discharge pipeline is arranged in the tank body; one end of the discharge pipeline penetrates into the bottom of the tank body, and the other end protrudes out of the tank body; a stirrer is arranged in the fermentation tank; the top of the fermentation tank is provided with an inoculation pipeline; the upper wall of the inoculation pipeline is provided with a sterilizing steam inlet pipe; the lower wall of the inoculation pipeline is provided with a steam condensate outlet pipe; the front end of the discharge pipeline is provided with a discharge port; the orifice diameter of the discharge port is smaller than that of the inoculation pipeline; and the discharge port can form an abutting joint with the orifice of the inoculation pipeline. The device can directly form an abutting joint with the inoculation port of the fermentation tank; the primary strain is inoculated into the fermentation tank by utilizing pressure difference without external contact, so the probability of infectious microbe infection is reduced to almost zero in the inoculation process; and thus, the fermentation process can proceed normally, and the production efficiency is greatly enhanced.

Description

A kind of edible fungus liquid fermentation first class inoculum is cultivated and aseptic inoculation equipment and technique
Technical field
The present invention relates to the equipment of a kind of edible fungus liquid fermentation first class inoculum cultivation and aseptic inoculation, and the technique adopting this equipment to inoculate, belong to biological fermentation field.
Background technology
In existing edible fungus species liquid fermentation process, one-level kind is all cultivate on shaking table with triangular flask, then utilizes Alcohol Flame ring at fermentation plant, opens the inoculation mouth of fermentor tank, pours the one-level kind in triangular flask into fermentor tank.In this process, the cleanliness factor of fermentation plant, fermentor tank are inoculated the sterility of mouth, whether cleaning-less bacteria infection has vital impact to the skill level of the cleanliness factor of triangular flask and operator on inoculating, each often above factor is careless slightly in seeded process will cause fermentor tank bacteria infection, the disaster of tank switching can be there is time serious, affect whole production process, cause huge financial loss to enterprise.
Summary of the invention
The application abandons present triangular flask shaking table culture process, utilize a kind of equipment, the cultivation of one-level kind is carried out in biochemical cultivation case, after having cultivated, the inoculation mouth of the discharge port of this equipment and fermentor tank is docked, first carry out sterilizing with steam docking port, utilize aseptic compressed air, by the first class inoculum press-in fermentor tank in this equipment.Carry out inoculation by this technique and can ensure that the inoculation sense rate of mixing of fermentor tank is less than 0.1%, be much higher than conventional vaccination ways.Specific as follows:
A kind of edible fungus liquid fermentation first class inoculum is cultivated and aseptic inoculation equipment, comprise tank body and fermentor tank, tank body top is provided with opening for feed, opening for feed is provided with charging lid, tank body top is also provided with air filter, and air filter is provided with compressed air inlet pipe, is also provided with discharge pipe in tank body, tank base is goed deep in discharge pipe one end, and the other end stretches out tank body;
Be provided with agitator in fermentor tank, fermentor tank top is provided with inoculation pipeline, and inoculation pipeline upper wall is provided with sterilizing steam inlet tube, and inoculation pipeline lower wall is provided with vapor condensation water outlet pipe;
Wherein, discharge pipe front end is provided with discharge port, and discharge port nozzle diameter is less than inoculation pipeline, and discharge port can dock with the mouth of pipe of inoculation pipeline.
Described tank body internal upper part is also provided with tank lifting ear, and tank body inner bottom part is also provided with magnetic stirring bar, and tank body lower part is also provided with magnetic stirring apparatus.
Described compressed air inlet pipe is provided with compressed air control valve, discharge pipe is provided with discharging control valve, inoculation pipeline is provided with inoculation pipe-line control valves, sterilizing steam inlet tube is provided with steam control valve, vapor condensation water outlet pipe is provided with vapor condensation control valve.
The present invention adopts aforesaid device to carry out the technique of inoculating and cultivating, and comprises the steps:
A. substratum is poured in tank body from opening for feed, the all valves on tank body and charging lid are closed, at 121 DEG C, sterilizing 30min, on aseptic platform, test tube kind is connect after naturally cooling, after inoculation, whole tank body is put on the magnetic stirring apparatus with biochemical cultivation case, cultivate, obtain first class inoculum;
B. cultivating is well docked on the inoculation pipeline of fermentor tank by the discharge port of tank body, open steam control valve, pass into steam from steam-pipe and carry out sterilizing, after sterilizing completes, open vapor condensation control valve, from vapor condensation water outlet pipe discharge water coolant, steam off control valve and vapor condensation control valve, open compressed air control valve, discharging control valve successively, inoculation pipe-line control valves, utilizes the pressure of aseptic compressed air by the first class inoculum access fermentor tank in tank body.
The present invention utilizes this equipment inoculation mouth that is direct and fermentor tank to dock, utilize pressure reduction to receive in fermentor tank by one-level kind, do not contact with the external world, in seeded process, the probability of bacteria infection reduces to almost nil, fermenting process can normally be carried out, and production efficiency is largely increased.
Accompanying drawing explanation
Fig. 1 is the front view of edible fungus liquid fermentation first class inoculum cultivation and aseptic inoculation equipment, wherein, and 1. tank body, 2. air filter, 3. compressed air inlet pipe, 4. compressed air control valve, 5. opening for feed, 6. charging lid, 7. discharge pipe, 8. discharging control valve, 9. discharge port, 10. tank lifting ear, 11. magnetic stirring bars, 12. magnetic stirring apparatuss, 13. steam control valves, 14. sterilizing steam inlet tubes, 15. vapor condensation control valves, 16. vapor condensation water outlet pipes, 17. inoculation pipelines, 18. inoculation pipe-line control valvess, 19. fermentor tanks.
Fig. 2 is the vertical view of tank body 1, wherein, and 1. tank body, 2. air filter, 3. compressed air inlet pipe, 4. compressed air control valve, 7. discharge pipe, 8. discharging control valve, 9. discharge port, 10. tank lifting ear.
Embodiment
Embodiment 1
A kind of edible fungus liquid fermentation first class inoculum is cultivated and aseptic inoculation equipment, comprise tank body 1 and fermentor tank 19, tank body 1 top is provided with opening for feed 5, opening for feed 5 is provided with charging lid 6, tank body 1 top is also provided with air filter 2, air filter 2 is provided with compressed air inlet pipe 3, is also provided with discharge pipe 7 in tank body 1, discharge pipe 7 one end is goed deep into bottom tank body 1, and the other end stretches out tank body 1;
Be provided with agitator in fermentor tank 19, fermentor tank 19 top is provided with inoculation pipeline 17, and inoculation pipeline 17 upper wall is provided with sterilizing steam inlet tube 14, and inoculation pipeline 17 lower wall is provided with vapor condensation water outlet pipe 16;
Wherein, discharge pipe 7 front end is provided with discharge port 9, and discharge port 9 nozzle diameter is less than inoculation pipeline 17,
And discharge port 9 can dock with the mouth of pipe of inoculation pipeline 17.
Described tank body 1 internal upper part is also provided with tank lifting ear 10, and be also provided with magnetic stirring bar 11 bottom 1 in tank body, tank body 1 bottom is also provided with magnetic stirring apparatus 12.
Compressed air inlet pipe 3 is provided with compressed air control valve 4, discharge pipe 7 is provided with discharging control valve 8, inoculation pipeline 17 is provided with inoculation pipe-line control valves 18, sterilizing steam inlet tube 14 is provided with steam control valve 13, vapor condensation water outlet pipe 16 is provided with vapor condensation control valve 15.
As shown in Figure 1, a kind of edible fungus liquid fermentation aseptic inoculation technique, in edible fungus liquid fermentative production, when cultivating first class inoculum, pour from the opening for feed 5 of tank body 1 substratum prepared into, and magnetic stirring bar 11 is put into tank body 1, cover charging lid 6, closes compression air control valve 4 and discharging control valve 8, put into sterilizing cabinet and carry out sterilizing 121 DEG C, 30min.On aseptic working platform, access test tube kind after cooling, the magnetic stirring apparatus 12 after inoculation, whole tank body 1 being put into biochemical cultivation case (being provided with socket) is cultivated.Cultivate well, take on fermentor tank, discharge port 9 is received fermentor tank feed pipe 17, opens steam control valve 13, by steam-pipe 14, steam is passed into fermentor tank inoculation mouth and carries out sterilizing, open water of condensation control valve 15 and discharge water coolant, then close water of condensation by-pass valve control 5 and steam control valve 14, open sterile air import 4, open discharging control valve 8, open the inoculation pipe-line control valves 18 of fermentor tank 19, utilize the pressure of sterile air by the one-level kind access fermentor tank 19 in tank 1.

Claims (4)

1. an edible fungus liquid fermentation first class inoculum is cultivated and aseptic inoculation equipment, comprise tank body (1) and fermentor tank (19), it is characterized in that, tank body (1) top is provided with opening for feed (5), opening for feed (5) is provided with charging lid (6), tank body (1) top is also provided with air filter (2), air filter (2) is provided with compressed air inlet pipe (3), discharge pipe (7) is also provided with in tank body (1), tank body (1) bottom is goed deep in discharge pipe (7) one end, and the other end stretches out tank body (1); Fermentor tank is provided with agitator in (19), and fermentor tank (19) top is provided with inoculation pipeline (17), and inoculation pipeline (17) upper wall is provided with sterilizing steam inlet tube (14), and inoculation pipeline (17) lower wall is provided with vapor condensation water outlet pipe (16);
Wherein, discharge pipe (7) front end is provided with discharge port (9), and discharge port (9) nozzle diameter is less than inoculation pipeline (17), and discharge port (9) can dock with the mouth of pipe of inoculation pipeline (17).
2. equipment according to claim 1, is characterized in that, tank body (1) internal upper part is also provided with tank lifting ear (10), and in tank body, (1) bottom is also provided with magnetic stirring bar (11), and tank body (1) bottom is also provided with magnetic stirring apparatus (12).
3. equipment according to claim 1, it is characterized in that, compressed air inlet pipe (3) is provided with compressed air control valve (4), discharge pipe (7) is provided with discharging control valve (8), inoculation pipeline (17) is provided with inoculation pipe-line control valves (18), sterilizing steam inlet tube (14) is provided with steam control valve (13), vapor condensation water outlet pipe (16) is provided with vapor condensation control valve (15).
4. edible fungus liquid fermentation first class inoculum is cultivated and an aseptic inoculation technique, it is characterized in that, comprises the steps:
A. substratum is poured in tank body from opening for feed, the all valves on tank body and charging lid are closed, at 121 DEG C, sterilizing 30min, on aseptic platform, test tube kind is connect after naturally cooling, after inoculation, whole tank body is put on the magnetic stirring apparatus with biochemical cultivation case, cultivate, obtain first class inoculum;
B. cultivating is well docked on the inoculation pipeline of fermentor tank by the discharge port of tank body, open steam control valve, pass into steam from steam-pipe and carry out sterilizing, after sterilizing completes, open vapor condensation control valve, from vapor condensation water outlet pipe discharge water coolant, steam off control valve and vapor condensation control valve, open compressed air control valve, discharging control valve successively, inoculation pipe-line control valves, utilizes the pressure of aseptic compressed air by the first class inoculum access fermentor tank in tank body.
CN201510313920.3A 2015-06-10 2015-06-10 A kind of edible fungus liquid fermentation first class inoculum culture and aseptic inoculation equipment and technique Expired - Fee Related CN104911096B (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505744A (en) * 2016-01-07 2016-04-20 盐城市雨露农业科技有限公司 Liquid strain seed tank and using method thereof
CN106172753A (en) * 2016-08-23 2016-12-07 山西维尔生物乳制品有限公司 A kind of liquid fermentation agent classification inoculation apparatus and method
CN106222078A (en) * 2016-08-03 2016-12-14 王卫华 A kind of biological fermentation tank
CN107502535A (en) * 2017-09-01 2017-12-22 苏州顺泰元虫草生物科技有限公司 A kind of high yield cordyceps sinensis culture systems
CN107988056A (en) * 2017-11-24 2018-05-04 成都凯成轻工药业装备有限公司 A kind of strain scene classification inoculation apparatus and method
CN108285862A (en) * 2018-04-18 2018-07-17 中国农业科学院农业资源与农业区划研究所 Microbial ferment device
CN108949517A (en) * 2017-05-19 2018-12-07 上海吉态来生物技术有限公司 A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe
CN110218638A (en) * 2019-07-02 2019-09-10 大连富森智能科技有限公司 A kind of spawn fermentation tank
CN110669659A (en) * 2019-07-05 2020-01-10 青海华牛生物科技有限公司 Matrimony vine ferment feed liquid inoculation jar
CN113229041A (en) * 2021-06-16 2021-08-10 绵阳菌源博文菌业有限公司 Domestic fungus liquid fermentation jar convenient to sterilization
CN115678749A (en) * 2022-10-17 2023-02-03 安琪酶制剂(宜昌)有限公司 Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method

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CN204151250U (en) * 2014-09-11 2015-02-11 湖北正佳微生物工程股份有限公司 A kind of feed supplement tank with steam sterilizer

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105505744A (en) * 2016-01-07 2016-04-20 盐城市雨露农业科技有限公司 Liquid strain seed tank and using method thereof
CN106222078A (en) * 2016-08-03 2016-12-14 王卫华 A kind of biological fermentation tank
CN106172753A (en) * 2016-08-23 2016-12-07 山西维尔生物乳制品有限公司 A kind of liquid fermentation agent classification inoculation apparatus and method
CN108949517A (en) * 2017-05-19 2018-12-07 上海吉态来生物技术有限公司 A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe
CN107502535A (en) * 2017-09-01 2017-12-22 苏州顺泰元虫草生物科技有限公司 A kind of high yield cordyceps sinensis culture systems
CN107988056A (en) * 2017-11-24 2018-05-04 成都凯成轻工药业装备有限公司 A kind of strain scene classification inoculation apparatus and method
CN108285862A (en) * 2018-04-18 2018-07-17 中国农业科学院农业资源与农业区划研究所 Microbial ferment device
CN110218638A (en) * 2019-07-02 2019-09-10 大连富森智能科技有限公司 A kind of spawn fermentation tank
CN110669659A (en) * 2019-07-05 2020-01-10 青海华牛生物科技有限公司 Matrimony vine ferment feed liquid inoculation jar
CN113229041A (en) * 2021-06-16 2021-08-10 绵阳菌源博文菌业有限公司 Domestic fungus liquid fermentation jar convenient to sterilization
CN113229041B (en) * 2021-06-16 2023-12-01 绵阳菌源博文菌业有限公司 Edible fungus liquid fermentation tank convenient to sterilize
CN115678749A (en) * 2022-10-17 2023-02-03 安琪酶制剂(宜昌)有限公司 Fermentation tank inoculation system convenient for sterilization and positive pressure liquid supply and use method

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