CN105505744A - Liquid strain seed tank and using method thereof - Google Patents
Liquid strain seed tank and using method thereof Download PDFInfo
- Publication number
- CN105505744A CN105505744A CN201610027407.2A CN201610027407A CN105505744A CN 105505744 A CN105505744 A CN 105505744A CN 201610027407 A CN201610027407 A CN 201610027407A CN 105505744 A CN105505744 A CN 105505744A
- Authority
- CN
- China
- Prior art keywords
- ball valve
- tank
- air
- liquid spawn
- sterilizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a liquid strain seed tank. The tank top is provided with an inoculating hole, a sterilization steam channel, a precise air filter, a purified air channel and an exhaust gas outlet, and the upper portion of a tank body is provided with an observation window. The invention further discloses a using method of the liquid strain seed tank. The using method comprises the steps that 1, the air-sterilizing precise filter performs sterilizing; 2, the air-sterilizing precise filter performs blow-drying; 3, a seed tank culture solution is sterilized; 4, air is introduced into the seed tank; 5, strains are inoculated; 6, sampling detection is performed; 7, a fermentation tank is inoculated. According to the liquid strain seed tank and the using method thereof, the liquid strain seed tank is separated from the liquid strain fermentation tank, sterilizing and inoculating are separately performed, and different liquid strain fermentation tanks can be inoculated into each liquid strain seed tank; through specific pipelines of the liquid strain seed tank, all operations such as sterilizing, air introducing, strain inoculating, sampling and liquid strain fermentation tank inoculating are convenient, and the pollution probability is decreased; meanwhile, the breaking action on strain balls is achieved, and a large quantity of hypha fragments are generated when the liquid strain fermentation tank is inoculated, so that rapid proliferation of mycelia in the liquid strain fermentation tank is facilitated.
Description
Technical field
The present invention relates to the equipment of a kind of yeast culture and inoculation, be specifically related to a kind of liquid spawn seeding tank, the invention still further relates to the using method of described liquid spawn seeding tank, belong to microorganism culturing preparation field.
Background technology
At present, seed tank culture bacterial classification is utilized to be prior art substantially in this area.But current prior art, complex operation when cultivating and inoculate, easily produces bacteriological infection, causes failure.
Summary of the invention
An object of the present invention is to provide a kind of liquid spawn seeding tank.
Liquid spawn seeding tank, has one to inoculate mouth 5 at tank deck, and sterilizing vapour passage 1, an air accurate filter 2, purify air passage 3 and waste gas outlet 4, has a viewing window 6 on tank body top, described sterilizing vapour passage comprises a steam inlet K1 be communicated with tank interior, steam inlet K1 there is ball valve F2, a lateral is had between ball valve F2 and steam inlet K1 entrance, lateral there is ball valve F1, be connected with air accurate filter by pipeline at steam inlet K1, air accurate filter 2 there is tensimeter B1, pipeline between steam inlet K1 and air accurate filter 2 there is ball valve F3, pipeline between ball valve F3 with air accurate filter 2 is connected ball valve F4 and air intlet K2 by a lateral, air accurate filter 2 is connected with ball valve F5, air accurate filter 2 and purify air between passage 3 and have vacuum breaker Z1, the order on passage 3 that purifies air is provided with ball valve F6 and ball valve F8, the passage 3 that purifies air is communicated with tank interior, ball valve F7 is connected with by a lateral between ball valve F6 and ball valve F8, waste gas outlet pipeline is arranged on tank deck, waste gas outlet pipeline is separately installed with tensimeter B2, ball valve F9, the vacuum breaker Z2 be connected by a lateral between tensimeter B2 with ball valve F9 and safety valve A1.
Liquid spawn seeding tank of the present invention, rational in infrastructure, can avoid polluting when spawn culture and inoculation by high degree.The specific pipeline of liquid spawn seeding tank, has crushing effect to bacterium ball, when inoculating liquid spawn fermentation tank, producing a large amount of mycelia fragment, being conducive to mycelium and breeding rapidly in liquid spawn fermentation tank.
Another object of the present invention is the using method providing described liquid spawn seeding tank, specifically comprises the steps:
Step one, air sterilization air accurate filter carry out sterilizing: ball valve F1, ball valve F2, ball valve F4, ball valve F7 close, ball valve F3, ball valve F6, ball valve F9 are logical, steam inlet K1 connects high-temperature steam, ball valve F5 gives vent to anger draining slightly, regulate ball valve F8 to make tensimeter B1 be stabilized in 0.14MPa, keep 30 minutes.
Step 2, air sterilization air accurate filter dry up: close ball valve F3, air intlet K2 connects uncontaminated air, pass ball valve F4, ball valve F7, close after ball valve F8 ventilates 1 minute and (prevent from forming vacuum, pipeline sucks nutrient solution and causes sterilizing not thorough), filter core is dried up, until the sterilizing of liquid spawn seeding tank terminates.
Step 3, liquid spawn seed tank culture liquid carry out sterilizing: pass ball valve F2, and after cultivating boiling, ball valve F1 ventilates slightly, regulate ball valve F9 to make tensimeter B2 be stabilized in 0.14MPa, keep 40 minutes, close ball valve F1, ball valve F2.Air sterilization air accurate filter dries up and carries out sterilizing with liquid spawn seed tank culture liquid and carry out simultaneously.
Step 4, liquid spawn seeding tank pass into air: when tensimeter B2 shows about 0.05MPa, close ball valve F5, ball valve F7, pass ball valve F8, regulate ball valve F9 to make tensimeter B2 be stabilized in 0.1MPa.
Step 5, access bacterial classification: when culture-liquid temp drops to about 25 degree, with pyrosphere protection inoculation mouth, access triangular flask bacterial classification.
Step 6, sampling detect: preparing to detect carrying out sampling the day before yesterday that liquid spawn fermentation tank is inoculated, closing ball valve F6, ball valve F9, under the protection of flame; slightly open ball valve F7, liquid spawn is flowed out automatically, completes sampling; after sampling terminates, valve returns to the state before sampling.Detect sampling, as pollution-free, namely liquid spawn seeding tank can be used for liquid spawn fermentation tank inoculation.
Step 7, liquid spawn fermentation tank to be inoculated: during liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank top bacterial classification access port and liquid spawn seeding tank steam inlet K1 is connected with silicone tube, open liquid spawn fermentation tank bacterial classification access port valve, regulate ball valve F1, make its draining of slightly giving vent to anger, until liquid spawn fermentation tank sterilizing terminates, close ball valve F1.Inoculate after liquid spawn fermentation tank cooling, close ball valve F9, pass ball valve F2, in liquid spawn seeding tank, liquid spawn is all automatically injected liquid spawn fermentation tank by silicone tube, and closing liquid strain fermentation tank top bacterial classification access port, completes inoculation work.
This liquid spawn seeding tank is suitable for be inoculated the liquid spawn fermentation tank of 200-5000L.
Volume is 5% of liquid spawn fermentation tank.
Generally speaking, following condition is needed when this liquid spawn seeding tank uses: 1, with or without the air source that oil is clean.2, the vapour source of more than pressure 0.2MPa is had.3, there is air-conditioning indoor.
The present invention has following advantage:
1, liquid spawn seeding tank is separated with liquid spawn fermentation tank, independent sterilizing, inoculation, and each liquid spawn seeding tank all can inoculate different liquid spawn fermentation tanks.If liquid spawn seeding tank pollutes, do not affect liquid spawn fermentation tank and normally use, lose less.
2, before liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank and liquid spawn seeding tank is connected with silicone tube, during liquid spawn fermentation tank sterilizing, sterilizing is carried out to inoculation pipeline simultaneously, seeded process contamination probability is zero, liquid spawn seeding tank as pollution-free, then liquid spawn fermentation tank success ratio of inoculation 100%.
3, innovate pipeline to connect, sterilizing steam-in doubles as bacterial classification outlet, and the passage that purifies air doubles as thief hatch, pipeline is unique succinct, easy to operate, without dead angle, sterilizing, ventilation, access bacterial classification, sampling and to operations such as liquid spawn fermentation tank inoculations can be completed easily.Bacterial classification outlet splits with thief hatch, can avoid thief hatch just in case pollute the impact on seeded process.
4, the specific pipeline of liquid spawn seeding tank, has crushing effect to bacterium ball, when inoculating liquid spawn fermentation tank, producing a large amount of mycelia fragment, being conducive to mycelium and breeding rapidly in liquid spawn fermentation tank.
Accompanying drawing explanation
Fig. 1 is the overall schematic of liquid spawn seeding tank of the present invention;
Fig. 2 is the sectional view of liquid spawn seeding tank of the present invention;
Fig. 3 is the structural representation of liquid spawn seeding tank capping of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is further illustrated.
As shown in accompanying drawing 1,2,3, liquid spawn seeding tank, has one to inoculate mouth 5 at tank deck, and sterilizing vapour passage 1, an air accurate filter 2, purify air passage 3 and waste gas outlet 4, has a viewing window 6 on tank body top, described sterilizing vapour passage comprises a steam inlet K1 be communicated with tank interior, steam inlet K1 there is ball valve F2, a lateral is had between ball valve F2 and steam inlet K1 entrance, lateral there is ball valve F1, be connected with air accurate filter by pipeline at steam inlet K1, air accurate filter 2 there is tensimeter B1, pipeline between steam inlet K1 and air accurate filter 2 there is ball valve F3, pipeline between ball valve F3 with air accurate filter 2 is connected ball valve F4 and air intlet K2 by a lateral, air accurate filter 2 is connected with ball valve F5, air accurate filter 2 and purify air between passage 3 and have vacuum breaker Z1, the order on passage 3 that purifies air is provided with ball valve F6 and ball valve F8, the passage 3 that purifies air is communicated with tank interior, ball valve F7 is connected with by a lateral between ball valve F6 and ball valve F8, waste gas outlet pipeline is arranged on tank deck, waste gas outlet pipeline is separately installed with tensimeter B2, ball valve F9, the vacuum breaker Z2 be connected by a lateral between tensimeter B2 with ball valve F9 and safety valve A1.
The using method of liquid spawn seeding tank, specifically comprises the steps:
Step one, air sterilization air accurate filter carry out sterilizing: ball valve F1, ball valve F2, ball valve F4, ball valve F7 close, ball valve F3, ball valve F6, ball valve F9 are logical, steam inlet K1 connects high-temperature steam, ball valve F5 gives vent to anger draining slightly, regulate ball valve F8 to make tensimeter B1 be stabilized in 0.14MPa, keep 30 minutes.
Step 2, air sterilization air accurate filter dry up: close ball valve F3, air intlet K2 connects uncontaminated air, pass ball valve F4, ball valve F7, close after ball valve F8 ventilates 1 minute and (prevent from forming vacuum, pipeline sucks nutrient solution and causes sterilizing not thorough), filter core is dried up, until the sterilizing of liquid spawn seeding tank terminates.
Step 3, liquid spawn seed tank culture liquid carry out sterilizing: pass ball valve F2, and after cultivating boiling, ball valve F1 ventilates slightly, regulate ball valve F9 to make tensimeter B2 be stabilized in 0.14MPa, keep 40 minutes, close ball valve F1, ball valve F2.Air sterilization air accurate filter dries up and carries out sterilizing with liquid spawn seed tank culture liquid and carry out simultaneously.
Step 4, liquid spawn seeding tank pass into air: when tensimeter B2 shows about 0.05MPa, close ball valve F5, ball valve F7, pass ball valve F8, regulate ball valve F9 to make tensimeter B2 be stabilized in 0.1MPa.
Step 5, access bacterial classification: when culture-liquid temp drops to about 25 degree, with pyrosphere protection inoculation mouth, access triangular flask bacterial classification.
Step 6, sampling detect: preparing to detect carrying out sampling the day before yesterday that liquid spawn fermentation tank is inoculated, closing ball valve F6, ball valve F9, under the protection of flame; slightly open ball valve F7, liquid spawn is flowed out automatically, completes sampling; after sampling terminates, valve returns to the state before sampling.Detect sampling, as pollution-free, namely liquid spawn seeding tank can be used for liquid spawn fermentation tank inoculation.
Step 7, liquid spawn fermentation tank to be inoculated: during liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank top bacterial classification access port and liquid spawn seeding tank steam inlet K1 is connected with silicone tube, open liquid spawn fermentation tank bacterial classification access port valve, regulate ball valve F1, make its draining of slightly giving vent to anger, until liquid spawn fermentation tank sterilizing terminates, close ball valve F1.Inoculate after liquid spawn fermentation tank cooling, close ball valve F9, pass ball valve F2, in liquid spawn seeding tank, liquid spawn is all automatically injected liquid spawn fermentation tank by silicone tube, and closing liquid strain fermentation tank top bacterial classification access port, completes inoculation work.
Within 2015, this liquid spawn seeding tank is through on probation, annual making bacterial classification 166 tank, success 158 tanks; Inoculation liquid spawn fermentation tank 133 tank, all successes.
Claims (2)
1. liquid spawn seeding tank, is characterized in that: have one to inoculate mouth at tank deck, and sterilizing vapour passage, an air accurate filter, purify air passage and waste gas outlet, has a viewing window on tank body top, described sterilizing vapour passage comprises a steam inlet K1 be communicated with tank interior, steam inlet K1 there is ball valve F2, a lateral is had between ball valve F2 and steam inlet K1 entrance, lateral there is ball valve F1, be connected with air accurate filter by pipeline at steam inlet K1, air accurate filter there is tensimeter B1, pipeline between steam inlet K1 and air accurate filter there is ball valve F3, pipeline between ball valve F3 with air accurate filter is connected ball valve F4 and air intlet K2 by a lateral, air accurate filter is connected with ball valve F5, air accurate filter and purify air between passage and have vacuum breaker Z1, the order on passage that purifies air is provided with ball valve F6 and ball valve F8, the passage that purifies air is communicated with tank interior, ball valve F7 is connected with by a lateral between ball valve F6 and ball valve F8, waste gas outlet pipeline is arranged on tank deck, waste gas outlet pipeline is separately installed with tensimeter B2, ball valve F9, the vacuum breaker Z2 be connected by a lateral between tensimeter B2 with ball valve F9 and safety valve Al.
2. the using method of liquid spawn seeding tank described in claim 1, is characterized in that comprising the steps:
Step one, air sterilization air accurate filter carry out sterilizing: ball valve F1, ball valve F2, ball valve F4, ball valve F7 close, ball valve F3, ball valve F6, ball valve F9 are logical, steam inlet K1 connects high-temperature steam, ball valve F5 gives vent to anger draining slightly, regulate ball valve F8 to make tensimeter B1 be stabilized in 0.14MPa, keep 30 minutes;
Step 2, air sterilization air accurate filter dry up: close ball valve F3, air intlet K2 connects uncontaminated air, pass ball valve F4, ball valve F7, close after ball valve F8 ventilates 1 minute and (prevent from forming vacuum, pipeline sucks nutrient solution and causes sterilizing not thorough), filter core is dried up, until the sterilizing of liquid spawn seeding tank terminates;
Step 3, liquid spawn seed tank culture liquid carry out sterilizing: pass ball valve F2, and after cultivating boiling, ball valve F1 ventilates slightly, regulate ball valve F9 to make tensimeter B2 be stabilized in 0.14MPa, keep 40 minutes, close ball valve F1, ball valve F2; Air sterilization air accurate filter dries up and carries out sterilizing with liquid spawn seed tank culture liquid and carry out simultaneously;
Step 4, liquid spawn seeding tank pass into air: when tensimeter B2 shows about 0.05MPa, close ball valve F5, ball valve F7, pass ball valve F8, regulate ball valve F9 to make tensimeter B2 be stabilized in 0.1MPa;
Step 5, access bacterial classification: when culture-liquid temp drops to about 25 degree, with pyrosphere protection inoculation mouth, access triangular flask bacterial classification;
Step 6, sampling detect: preparing to detect carrying out sampling the day before yesterday that liquid spawn fermentation tank is inoculated, closing ball valve F6, ball valve F9, under the protection of flame, slightly open ball valve F7, liquid spawn is flowed out automatically, completes sampling, after sampling terminates, valve returns to the state before sampling; Detect sampling, as pollution-free, namely liquid spawn seeding tank can be used for liquid spawn fermentation tank inoculation;
Step 7, liquid spawn fermentation tank to be inoculated: during liquid spawn fermentation tank sterilizing, liquid spawn fermentation tank top bacterial classification access port and liquid spawn seeding tank steam inlet K1 is connected with silicone tube, open liquid spawn fermentation tank bacterial classification access port valve, regulate ball valve F1, make its draining of slightly giving vent to anger, until liquid spawn fermentation tank sterilizing terminates, close ball valve F1; Inoculate after liquid spawn fermentation tank cooling, close ball valve F9, pass ball valve F2, in liquid spawn seeding tank, liquid spawn is all automatically injected liquid spawn fermentation tank by silicone tube, and closing liquid strain fermentation tank top bacterial classification access port, completes inoculation work.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610027407.2A CN105505744A (en) | 2016-01-07 | 2016-01-07 | Liquid strain seed tank and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610027407.2A CN105505744A (en) | 2016-01-07 | 2016-01-07 | Liquid strain seed tank and using method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105505744A true CN105505744A (en) | 2016-04-20 |
Family
ID=55714051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610027407.2A Pending CN105505744A (en) | 2016-01-07 | 2016-01-07 | Liquid strain seed tank and using method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105505744A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172753A (en) * | 2016-08-23 | 2016-12-07 | 山西维尔生物乳制品有限公司 | A kind of liquid fermentation agent classification inoculation apparatus and method |
| CN107058089A (en) * | 2017-03-24 | 2017-08-18 | 大连富森智能科技有限公司 | Liquid spawn fermentation tank |
| CN107744693A (en) * | 2017-10-24 | 2018-03-02 | 翔天农业开发集团股份有限公司 | A kind of aseptic filtration method of air in liquid fungus seed |
| CN107794227A (en) * | 2017-10-26 | 2018-03-13 | 翔天农业开发集团股份有限公司 | A kind of sterile carrying method of the one-to-many pipeline of liquid spawn |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
| CN202989118U (en) * | 2012-12-17 | 2013-06-12 | 福建省建阳武夷味精有限公司 | First-level seed fermentation tank |
| CN203319996U (en) * | 2013-05-10 | 2013-12-04 | 佛山金葵子植物营养有限公司 | Fermentation tank |
| CN203487144U (en) * | 2013-07-17 | 2014-03-19 | 南京朗恩生物科技有限公司 | Air balance pipeline system of fermentation tank |
| CN204611361U (en) * | 2014-12-15 | 2015-09-02 | 李甲新 | A kind of flashlight with razor function |
| CN104911096A (en) * | 2015-06-10 | 2015-09-16 | 湖北五林中地农业科技有限公司 | Edible fungus liquid fermentation primary strain culture and sterile inoculation device and technique |
| CN205313525U (en) * | 2016-01-07 | 2016-06-15 | 盐城市雨露农业科技有限公司 | Liquid spawn dona tub |
-
2016
- 2016-01-07 CN CN201610027407.2A patent/CN105505744A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
| CN202989118U (en) * | 2012-12-17 | 2013-06-12 | 福建省建阳武夷味精有限公司 | First-level seed fermentation tank |
| CN203319996U (en) * | 2013-05-10 | 2013-12-04 | 佛山金葵子植物营养有限公司 | Fermentation tank |
| CN203487144U (en) * | 2013-07-17 | 2014-03-19 | 南京朗恩生物科技有限公司 | Air balance pipeline system of fermentation tank |
| CN204611361U (en) * | 2014-12-15 | 2015-09-02 | 李甲新 | A kind of flashlight with razor function |
| CN104911096A (en) * | 2015-06-10 | 2015-09-16 | 湖北五林中地农业科技有限公司 | Edible fungus liquid fermentation primary strain culture and sterile inoculation device and technique |
| CN205313525U (en) * | 2016-01-07 | 2016-06-15 | 盐城市雨露农业科技有限公司 | Liquid spawn dona tub |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172753A (en) * | 2016-08-23 | 2016-12-07 | 山西维尔生物乳制品有限公司 | A kind of liquid fermentation agent classification inoculation apparatus and method |
| CN107058089A (en) * | 2017-03-24 | 2017-08-18 | 大连富森智能科技有限公司 | Liquid spawn fermentation tank |
| CN107744693A (en) * | 2017-10-24 | 2018-03-02 | 翔天农业开发集团股份有限公司 | A kind of aseptic filtration method of air in liquid fungus seed |
| CN107744693B (en) * | 2017-10-24 | 2018-11-20 | 翔天农业开发集团股份有限公司 | A kind of aseptic filtration method of air in liquid fungus seed |
| CN107794227A (en) * | 2017-10-26 | 2018-03-13 | 翔天农业开发集团股份有限公司 | A kind of sterile carrying method of the one-to-many pipeline of liquid spawn |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105505744A (en) | Liquid strain seed tank and using method thereof | |
| CN202077430U (en) | Fermentation tank with sterilizer at outer part | |
| CN203319996U (en) | Fermentation tank | |
| CN203490092U (en) | Vacuum sampling system | |
| CN102604836A (en) | Production method and device for preventing chytrid pollution in haematococcus pluvialis | |
| CN205313525U (en) | Liquid spawn dona tub | |
| CN104132940A (en) | Convenient observation method of orchid mycorrhiza microstructure | |
| CN206298591U (en) | A kind of microorganism classification inoculation apparatus with pressure | |
| KR20020012084A (en) | mushroom liquid spawn fermanter | |
| CN201389209Y (en) | Ultraviolet sterilization system of air needed by culture environment of needle mushroom liquid spawn | |
| CN202744548U (en) | Horizontal solid fermentation tank | |
| CN106967591B (en) | A kind of clinical microbiology anaerobe samples storage bottle | |
| CN202968544U (en) | Continuous fermentation propagation device for reducing contamination probability | |
| CN103205359B (en) | Sterile culture detection device | |
| CN204569894U (en) | Novel microorganism fermentor tank | |
| CN216404380U (en) | Fermentation cylinder positive pressure type inoculation system | |
| CN204661695U (en) | A kind of sampling unit detecting fermentative production sterile air | |
| CN205152208U (en) | Bacterium fermentation cultivation's novel inoculation device | |
| CN208995495U (en) | A kind of microculture box of good seal performance | |
| CN104756868B (en) | Herba Dendrobii inoculation device and inoculation method | |
| CN216960964U (en) | Velvet antler mushroom fermentation tank positive pressure inoculation system | |
| CN208949262U (en) | Microbial fermentation tank body | |
| CN203212575U (en) | Sterile culture detection device | |
| CN203212557U (en) | Dirt discharging device of first-grade fermentation tank of enzyme preparation | |
| CN203451526U (en) | Safety inoculating device of liquid fermentation tank |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160420 |