CN107794227A - A kind of sterile carrying method of the one-to-many pipeline of liquid spawn - Google Patents

A kind of sterile carrying method of the one-to-many pipeline of liquid spawn Download PDF

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Publication number
CN107794227A
CN107794227A CN201711011234.6A CN201711011234A CN107794227A CN 107794227 A CN107794227 A CN 107794227A CN 201711011234 A CN201711011234 A CN 201711011234A CN 107794227 A CN107794227 A CN 107794227A
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China
Prior art keywords
silica gel
gel hose
tank
fermentation tank
seeding tank
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Pending
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CN201711011234.6A
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Chinese (zh)
Inventor
汪建党
侯艳平
肖俊培
张金霞
黄晨阳
隋杰
史振霞
闫训友
侯晓强
韩美玲
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Xiang Tian Agricultural Development Group Co Ltd
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Xiang Tian Agricultural Development Group Co Ltd
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Priority to CN201711011234.6A priority Critical patent/CN107794227A/en
Publication of CN107794227A publication Critical patent/CN107794227A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The present invention relates to a kind of sterile carrying method of the one-to-many pipeline of liquid spawn, it comprises the following steps, and silica gel hose is connected with all of the port of 1.n port valves, cotton environmental sealing is used at the port of silica gel hose；2. the discharging opening of seeding tank keeps sterilizing by heat extraction steam；The charging aperture of 3 fermentation tanks keeps sterilizing by heat extraction steam；4. it will be less than or equal to（n‑1）Individual fermentation tank and the silica gel hose orifice of n port valves, seeding tank are then not connected with a silica gel hose orifice of fermentation tank with n port valves；When 5 silica gel hoses connect with seeding tank, the discharging opening of seeding tank and the junction of silica gel hose are roasted with flame flame envelope, the vapours of seeding tank is then turned off, the cotton for being wrapped in the silica gel hose is removed under the protection of flame；When 6 silica gel hoses connect with fermentation tank, operated with step 5 similar approach.Using carrying method of the present invention, strain conveying is carried out between seeding tank and fermentation tank, the pollution rate of strain substantially reduces.

Description

A kind of sterile carrying method of the one-to-many pipeline of liquid spawn
Technical field
The present invention relates to liquid spawn to transport conveying technology field, especially a kind of one-to-many pipeline of liquid spawn Sterile carrying method.
Background technology
Big ton, which holds fermentation tank production edible fungi liquid strain, has batch production, the homogeneous advantage low with cost of quality, will It is widely used as edible fungus species production new technology.But when needing to convey or be dispensed into multiple fermentation tanks, it is therein miscellaneous Bacterium pollution is a major challenge.In addition, when a seeding tank corresponds to multiple fermentation tanks, and seed strain is conveyed to it, how Flexibly selection conveyance conduit, definitely avoids polluting, and liquid spawn production has to the engineering technology problem of solution..It is edible Substantial amounts of mycelium pellet and exocellular polysaccharide in bacteria liquid strain, poor fluidity, flowing is slow, flow velocity poor controllability, delivery ratio bacterium and The difficulty of the unicellular microorganism liquid spawn such as yeast greatly increases, and pollution probability also greatly increases compared with unicellular microorganism.Cause This, the conveying of edible fungi liquid strain turns into the key of its success or not.
The content of the invention
It is an object of the invention to provide a kind of sterile carrying method of the one-to-many pipeline of liquid spawn so that a kind Sub- tank is conveyed in the course of conveying of multiple fermentation tanks, reduces the possibility of pollution of strain to greatest extent.
To achieve the above object, the present invention comprises the following steps,
（1）Silica gel hose will be connected with all of the port of n port valves, is wrapped up at the port of the silica gel hose with cotton close Envelope, then it is placed in high-pressure sterilizing pot and preserves in the lump, the temperature of the high-temperature sterilization is 122-125 DEG C, pressure 0.11- 0.15MPa；
（2）Seeding tank carries out high-temperature sterilization before n port valves are connected in tank, the discharging opening of seeding tank is kept by heat extraction steam Sterilizing；
（3）Fermentation tank carries out high-temperature sterilization before n port valves are connected in tank, the charging aperture of fermentation tank is kept by heat extraction steam Sterilizing；
（4）Connection purpose is to be less than or equal to（n-1）Individual fermentation tank and the silica gel hose orifice of n port valves, seeding tank is then A silica gel hose orifice of fermentation tank is not connected with n port valves；
（5）When silica gel hose connects with seeding tank, first the discharging opening of seeding tank is contacted with silica gel hose to be connected, uses flame The discharging opening of flame envelope barbecue seeding tank and the junction of silica gel hose, the vapours of seeding tank is then turned off, in the protection of flame It is lower to remove the cotton for being wrapped in the silica gel hose, realize that seeding tank docks with the sterile of n port valves.
（6）When silica gel hose connects with fermentation tank, first the charging aperture of fermentation tank is contacted with silica gel hose to be connected, used The charging aperture of flame flame envelope barbecue fermentation tank and the junction of silica gel hose, the vapours of fermentation tank is then turned off, in flame Protection is lower to remove the cotton for being wrapped in the silica gel hose, realizes that fermentation tank docks with the sterile of n port valves.
Further, the silica gel hose internal diameter is 1cm.
Further, the step（3）The temperature of middle fermentation tank high-temperature sterilization is 122-125 DEG C, pressure 0.11- 0.15MPa。
Further, the step（2）The temperature of middle seeding tank high-temperature sterilization is 122-125 DEG C, pressure 0.11- 0.15MPa。
Further, the n port valves are triple valve.
Further, the n port valves are five-way valve.
Good effect of the present invention is as follows：
Using carrying method of the present invention, strain conveying is carried out between seeding tank and fermentation tank, the pollution rate of strain substantially reduces.
The inventive method production cost substantially reduces, and is easy to peasant household's large-scale promotion, easy to operate, applied widely.
Embodiment
To embodiments of the invention be made with further narration in detail below.
Embodiment 1
The embodiment of the present invention comprises the following steps, and n port valves are 3 port valves in the present embodiment.
（1）Between connection, silica gel hose will be connected with all of the port of triple valve, it is equal at the port of the silica gel hose With cotton environmental sealing, then it is placed in high-pressure sterilizing pot and preserves in the lump, the temperature of the high-temperature sterilization is 122 DEG C, pressure For 0.15MPa, the high-temperature sterilization time is more than 30 minutes；The silica gel hose internal diameter is 1cm.
（2）Seeding tank carries out high-temperature sterilization before triple valve is connected in tank, the discharging opening of seeding tank passes through heat extraction steam To keep sterilizing；The temperature of the high-temperature sterilization is 123 DEG C, pressure 0.11MPa, and the high-temperature sterilization time is more than 30 minutes i.e. Can；
（3）Fermentation tank carries out high-temperature sterilization before triple valve is connected in tank, the charging aperture of fermentation tank is protected by heat extraction steam Hold sterilizing；The temperature of the high-temperature sterilization is 125 DEG C, pressure 0.13MPa, and the high-temperature sterilization time is more than 30 minutes；
（4）It is that the silica gel hose orifice of the fermentation tank of 1 or 2 and triple valve, the present embodiment are connected into 2 to connect purpose Fermentation tank, seeding tank are then not connected with a silica gel hose orifice of fermentation tank with triple valve；
（5）When silica gel hose connects with seeding tank, first the discharging opening of seeding tank is contacted with silica gel hose to be connected, uses flame The discharging opening of flame envelope barbecue seeding tank and the junction of silica gel hose, the vapours of seeding tank is then turned off, in the protection of flame It is lower to remove the cotton for being wrapped in the silica gel hose, realize that seeding tank docks with the sterile of triple valve.
（6）When silica gel hose connects with fermentation tank, first the charging aperture of fermentation tank is contacted with silica gel hose to be connected, used The charging aperture of flame flame envelope barbecue fermentation tank and the junction of silica gel hose, the vapours of fermentation tank is then turned off, in flame Protection is lower to remove the cotton for being wrapped in the silica gel hose, realizes that fermentation tank docks with the sterile of triple valve.
After connection, the import of the triple valve connects with seeding tank, two outlets and 2 fermentations respectively of the triple valve Tank connects.It is achieved in the sterile conveying of the one-to-many pipeline of liquid spawn.
Embodiment 2
The embodiment of the present invention comprises the following steps, and n port valves are five-way valve in the present embodiment.
（1）Silica gel hose will be connected with all of the port of five-way valve, the bale of cotton is used at the port of the silica gel hose Sealing is wrapped up in, is then placed in high-pressure sterilizing pot and preserves in the lump, the temperature of the high-temperature sterilization is 123 DEG C, and pressure is 0.11MPa；The silica gel hose internal diameter is 1cm.
（2）Seeding tank carries out high-temperature sterilization before five-way valve is connected in tank, the discharging opening of seeding tank passes through heat extraction steam To keep sterilizing；The temperature of the high-temperature sterilization is 122 DEG C, pressure 0.15MPa；
（3）Fermentation tank carries out high-temperature sterilization before five-way valve is connected in tank, the charging aperture of fermentation tank is protected by heat extraction steam Hold sterilizing；The temperature of the high-temperature sterilization is 122 DEG C, pressure 0.11MPa；
（4）It is by the silica gel hose orifice of the fermentation tank of 1,2,3 or 4 and five-way valve, this implementation to connect purpose Example 3 fermentation tanks of connection, seeding tank are then not connected with a silica gel hose orifice of fermentation tank with five-way valve；
（5）When silica gel hose connects with seeding tank, first the discharging opening of seeding tank is contacted with silica gel hose to be connected, uses flame The discharging opening of flame envelope barbecue seeding tank and the junction of silica gel hose, the vapours of seeding tank is then turned off, in the protection of flame It is lower to remove the cotton for being wrapped in the silica gel hose, realize that seeding tank docks with the sterile of five-way valve.
（6）When silica gel hose connects with fermentation tank, first the charging aperture of fermentation tank is contacted with silica gel hose to be connected, used The charging aperture of flame flame envelope barbecue fermentation tank and the junction of silica gel hose, the vapours of fermentation tank is then turned off, in flame Protection is lower to remove the cotton for being wrapped in the silica gel hose, realizes that fermentation tank docks with the sterile of five-way valve.
After connection, the import of the five-way valve connects with seeding tank, and the outlet of the five-way valve connects with 3 fermentation tanks respectively It is logical.It is achieved in the sterile conveying of the one-to-many pipeline of liquid spawn.
Embodiment 3
The embodiment of the present invention comprises the following steps, and n port valves are five-way valve in the present embodiment.
（1）Silica gel hose will be connected with all of the port of five-way valve, the bale of cotton is used at the port of the silica gel hose Sealing is wrapped up in, is then placed in high-pressure sterilizing pot and preserves in the lump, the temperature of the high-temperature sterilization is 125 DEG C, and pressure is 0.12MPa；The silica gel hose internal diameter is 1cm.
（2）Seeding tank carries out high-temperature sterilization before five-way valve is connected in tank, the discharging opening of seeding tank passes through heat extraction steam To keep sterilizing；The temperature of the high-temperature sterilization is 125 DEG C, pressure 0.13MPa；
（3）Fermentation tank carries out high-temperature sterilization before five-way valve is connected in tank, the charging aperture of fermentation tank is protected by heat extraction steam Hold sterilizing；The temperature of the high-temperature sterilization is 124 DEG C, pressure 0.15MPa；
（4）It is by the silica gel hose orifice of the fermentation tank of 1,2,3 or 4 and five-way valve, this implementation to connect purpose Example 4 fermentation tanks of connection, seeding tank are then not connected with a silica gel hose orifice of fermentation tank with five-way valve；
（5）When silica gel hose connects with seeding tank, first the discharging opening of seeding tank is contacted with silica gel hose to be connected, uses flame The discharging opening of flame envelope barbecue seeding tank and the junction of silica gel hose, the vapours of seeding tank is then turned off, in the protection of flame It is lower to remove the cotton for being wrapped in the silica gel hose, realize that seeding tank docks with the sterile of five-way valve.
（6）When silica gel hose connects with fermentation tank, first the charging aperture of fermentation tank is contacted with silica gel hose to be connected, used The charging aperture of flame flame envelope barbecue fermentation tank and the junction of silica gel hose, the vapours of fermentation tank is then turned off, in flame Protection is lower to remove the cotton for being wrapped in the silica gel hose, realizes that fermentation tank docks with the sterile of five-way valve.
After connection, the import of the five-way valve connects with seeding tank, and the outlet of the five-way valve connects with 4 fermentation tanks respectively It is logical.It is achieved in the sterile conveying of the one-to-many pipeline of liquid spawn.
By test, using carrying method of the present invention, strain conveying, the dirt of strain are carried out between seeding tank and fermentation tank Dye rate substantially reduces, and pollution rate is only 1%-0.5%.
The inventive method production cost substantially reduces, and is easy to peasant household's large-scale promotion, easy to operate, applied widely.
The foregoing description of the disclosed embodiments, professional and technical personnel in the field are enable to realize or using the present invention. A variety of modifications to these embodiments will be apparent for those skilled in the art, as defined herein General Principle can be realized in other embodiments in the case where not departing from the spirit or scope of patent of the present invention.Therefore, originally Invention is not intended to be limited to the embodiments shown herein, and is to fit to and principles disclosed herein and features of novelty Consistent most wide scope.

Claims (6)

  1. A kind of 1. sterile carrying method of the one-to-many pipeline of liquid spawn, it is characterised in that：Comprise the following steps,
    （1）Silica gel hose will be connected with all of the port of n port valves, is wrapped up at the port of the silica gel hose with cotton close Envelope, then it is placed in high-pressure sterilizing pot and preserves in the lump, the temperature of the high-temperature sterilization is 122-125 DEG C, pressure 0.11- 0.15MPa；
    （2）Seeding tank carries out high-temperature sterilization before n port valves are connected in tank, the discharging opening of seeding tank is kept by heat extraction steam Sterilizing；
    （3）Fermentation tank carries out high-temperature sterilization before n port valves are connected in tank, the charging aperture of fermentation tank is kept by heat extraction steam Sterilizing；
    （4）Connection purpose is to be less than or equal to（n-1）Individual fermentation tank and the silica gel hose orifice of n port valves, seeding tank is then A silica gel hose orifice of fermentation tank is not connected with n port valves；
    （5）When silica gel hose connects with seeding tank, first the discharging opening of seeding tank is contacted with silica gel hose to be connected, uses flame The discharging opening of flame envelope barbecue seeding tank and the junction of silica gel hose, the vapours of seeding tank is then turned off, in the protection of flame It is lower to remove the cotton for being wrapped in the silica gel hose, realize that seeding tank docks with the sterile of n port valves；
    （6）When silica gel hose connects with fermentation tank, first the charging aperture of fermentation tank is contacted with silica gel hose to be connected, uses flame The charging aperture of flame envelope barbecue fermentation tank and the junction of silica gel hose, the vapours of fermentation tank is then turned off, in the protection of flame It is lower to remove the cotton for being wrapped in the silica gel hose, realize that fermentation tank docks with the sterile of n port valves.
  2. A kind of 2. sterile carrying method of the one-to-many pipeline of liquid spawn according to claim 1, it is characterised in that： The silica gel hose internal diameter is 1cm.
  3. A kind of 3. sterile carrying method of the one-to-many pipeline of liquid spawn according to claim 1, it is characterised in that： The step（3）The temperature of middle fermentation tank high-temperature sterilization is 122-125 DEG C, pressure 0.11-0.15MPa.
  4. A kind of 4. sterile carrying method of the one-to-many pipeline of liquid spawn according to claim 1, it is characterised in that： The step（2）The temperature of middle seeding tank high-temperature sterilization is 122-125 DEG C, pressure 0.11-0.15MPa.
  5. A kind of 5. sterile carrying method of the one-to-many pipeline of liquid spawn according to claim 1, it is characterised in that： The n port valves are triple valve.
  6. A kind of 6. sterile carrying method of the one-to-many pipeline of liquid spawn according to claim 1, it is characterised in that： The n port valves are five-way valve.
CN201711011234.6A 2017-10-26 2017-10-26 A kind of sterile carrying method of the one-to-many pipeline of liquid spawn Pending CN107794227A (en)

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Application Number Priority Date Filing Date Title
CN201711011234.6A CN107794227A (en) 2017-10-26 2017-10-26 A kind of sterile carrying method of the one-to-many pipeline of liquid spawn

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Application Number Priority Date Filing Date Title
CN201711011234.6A CN107794227A (en) 2017-10-26 2017-10-26 A kind of sterile carrying method of the one-to-many pipeline of liquid spawn

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CN107794227A true CN107794227A (en) 2018-03-13

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2075203U (en) * 1990-09-07 1991-04-17 梁希珍 Liquid injecting device for biological ferment
WO2002032816A1 (en) * 2000-10-13 2002-04-25 Inbionet Corporation Apparatus and process for bioconverting high-concentrated organic waste
CN105505744A (en) * 2016-01-07 2016-04-20 盐城市雨露农业科技有限公司 Liquid strain seed tank and using method thereof
CN205902434U (en) * 2016-08-19 2017-01-25 四川宝生药业发展有限公司 Deep culture apparatus of tubular

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2075203U (en) * 1990-09-07 1991-04-17 梁希珍 Liquid injecting device for biological ferment
WO2002032816A1 (en) * 2000-10-13 2002-04-25 Inbionet Corporation Apparatus and process for bioconverting high-concentrated organic waste
CN105505744A (en) * 2016-01-07 2016-04-20 盐城市雨露农业科技有限公司 Liquid strain seed tank and using method thereof
CN205902434U (en) * 2016-08-19 2017-01-25 四川宝生药业发展有限公司 Deep culture apparatus of tubular

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Application publication date: 20180313