CN107794227A - A kind of sterile carrying method of the one-to-many pipeline of liquid spawn - Google Patents
A kind of sterile carrying method of the one-to-many pipeline of liquid spawn Download PDFInfo
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Abstract
Description
技术领域technical field
本发明涉及液体菌种运输输送技术领域，尤其是一种液体菌种一对多管道输送的无菌输送方法。The invention relates to the technical field of transportation and delivery of liquid bacteria, in particular to an aseptic delivery method for one-to-many pipeline delivery of liquid bacteria.
背景技术Background technique
大吨容发酵罐生产食用菌液体菌种具有批量生产、品质均一和成本低的优势，将成为食用菌菌种生产新技术广为应用。但是，当需要输送或分装到多个发酵罐时，其中的杂菌污染是一大挑战。此外，当一个种子罐对应多个发酵罐，向其输送种子菌种时，如何能够灵活选择输送管道，绝对避免污染，也是液体菌种生产必须要解决的工程技术问题。。食用菌液体菌种中大量的菌丝球和胞外多糖，流动性差，流动慢，流速可控性差，输送比细菌和酵母等单细胞微生物液体菌种的难度大大增加，污染几率较单细胞微生物也大大增加。因此，食用菌液体菌种的输送成为其成功与否的关键。The production of edible fungus liquid strains in large-ton fermentation tanks has the advantages of mass production, uniform quality and low cost, and will become a new technology for the production of edible fungi strains. However, bacterial contamination is a challenge when conveying or distributing to multiple fermenters. In addition, when one seed tank corresponds to multiple fermenters, how to flexibly select the delivery pipeline and absolutely avoid pollution is also an engineering and technical problem that must be solved in the production of liquid strains when transporting seed strains to them. . There are a large number of mycelium balls and exopolysaccharides in edible mushroom liquid strains, which have poor fluidity, slow flow, and poor controllability of flow rate. also greatly increased. Therefore, the delivery of edible fungus liquid spawn becomes the key to its success.
发明内容Contents of the invention
本发明的目的是提供一种液体菌种一对多管道输送的无菌输送方法，使得一个种子罐输送给多个发酵罐的输送过程中，最大限度降低了菌种的污染可能性。The purpose of the present invention is to provide an aseptic delivery method for one-to-many pipeline delivery of liquid bacteria, so that the possibility of contamination of bacteria can be minimized during the delivery process from one seed tank to multiple fermentation tanks.
为实现上述目的，本发明包括以下步骤，To achieve the above object, the present invention comprises the following steps,
（1）将n通阀的所有端口处连接有硅胶软管，所述硅胶软管的端口处均用棉花包裹密封，然后一并放置在高压灭菌锅内保存，所述高温灭菌的温度为122-125℃，压力为0.11-0.15MPa；(1) Connect all ports of the n-way valve with silicone hoses, and wrap and seal the ports of the silicone hoses with cotton, and then store them together in an autoclave. The temperature of the high-temperature sterilization 122-125°C, pressure 0.11-0.15MPa;
（2）种子罐在连通n通阀之前罐内进行高温灭菌，种子罐的出料口通过排热蒸汽来保持灭菌；(2) The seed tank is sterilized at high temperature before it is connected to the n-way valve, and the outlet of the seed tank is kept sterilized by exhausting steam;
（3）发酵罐在连通n通阀之前罐内进行高温灭菌，发酵罐的进料口通过排热蒸汽来保持灭菌；(3) The fermenter is sterilized at high temperature before the n-way valve is connected, and the feed port of the fermenter is kept sterilized by exhausting steam;
（4）连通目的为将小于等于（n-1）个的发酵罐与n通阀的硅胶软管接口连通，种子罐则与n通阀未连接发酵罐的一个硅胶软管接口连通；(4) The purpose of the connection is to connect fermentors less than or equal to (n-1) with the silicone hose interface of the n-way valve, and the seed tank is connected with a silicone hose interface of the n-way valve that is not connected to the fermenter;
（5）硅胶软管与种子罐连通时，先将种子罐的出料口与待连接的硅胶软管接触，用火焰外焰烧烤种子罐的出料口和硅胶软管的连接处，然后关掉种子罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现种子罐与n通阀的无菌对接。(5) When the silicone hose is connected to the seed tank, first contact the outlet of the seed tank with the silicone hose to be connected, and use the flame to grill the connection between the outlet of the seed tank and the silicone hose, and then close the Remove the hot steam from the seed tank, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the aseptic docking of the seed tank and the n-way valve.
（6）硅胶软管与发酵罐连通时，先将发酵罐的进料口与待连接的硅胶软管接触，用火焰外焰烧烤发酵罐的进料口和硅胶软管的连接处，然后关掉发酵罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现发酵罐与n通阀的无菌对接。(6) When the silicone hose is connected to the fermenter, first contact the feed port of the fermenter with the silicone hose to be connected, grill the connection between the feed port of the fermenter and the silicone hose with an external flame, and then close the Remove the hot steam from the fermenter, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the sterile docking of the fermenter and the n-way valve.
进一步地，所述硅胶软管内径为1cm。Further, the inner diameter of the silicone hose is 1 cm.
进一步地，所述步骤（3）中发酵罐高温灭菌的温度为122-125℃，压力为0.11-0.15MPa。Further, in the step (3), the temperature of the high-temperature sterilization of the fermenter is 122-125° C., and the pressure is 0.11-0.15 MPa.
进一步地，所述步骤（2）中种子罐高温灭菌的温度为122-125℃，压力为0.11-0.15MPa。Further, in the step (2), the temperature of the high-temperature sterilization of the seed tank is 122-125° C., and the pressure is 0.11-0.15 MPa.
进一步地，所述n通阀为三通阀。Further, the n-way valve is a three-way valve.
进一步地，所述n通阀为五通阀。Further, the n-way valve is a five-way valve.
本发明积极效果如下：The positive effect of the present invention is as follows:
使用本发明输送方法，在种子罐与发酵罐之间进行菌种输送，菌种的污染率大大降低。By using the conveying method of the present invention, strains are conveyed between the seed tank and the fermentation tank, and the pollution rate of strains is greatly reduced.
本发明方法生产成本大大降低，便于农户大规模推广，操作简便，适用范围广。The method of the invention greatly reduces the production cost, is convenient for farmers to popularize on a large scale, is easy to operate, and has a wide application range.
具体实施方式Detailed ways
下面将对本发明的实施例作进一步的详细叙述。Embodiments of the present invention will be further described in detail below.
实施例1Example 1
本发明实施例包括以下步骤，本实施例中n通阀为3通阀。The embodiment of the present invention includes the following steps. In this embodiment, the n-way valve is a 3-way valve.
（1）连通之间，将三通阀的所有端口处连接有硅胶软管，所述硅胶软管的端口处均用棉花包裹密封，然后一并放置在高压灭菌锅内保存，所述高温灭菌的温度为122℃，压力为0.15MPa，高温灭菌时间大于30分钟即可；所述硅胶软管内径为1cm。(1) Between the connections, connect all the ports of the three-way valve with silicone hoses. The ports of the silicone hoses are wrapped and sealed with cotton, and then placed together in an autoclave for storage. The high temperature The sterilization temperature is 122° C., the pressure is 0.15 MPa, and the high-temperature sterilization time is more than 30 minutes; the inner diameter of the silicone hose is 1 cm.
（2）种子罐在连通三通阀之前罐内进行高温灭菌，种子罐的出料口通过排热蒸汽来保持灭菌；所述高温灭菌的温度为123℃，压力为0.11MPa，高温灭菌时间大于30分钟即可；(2) The seed tank is subjected to high-temperature sterilization in the tank before connecting the three-way valve, and the outlet of the seed tank is kept sterilized by exhausting heat steam; the temperature of the high-temperature sterilization is 123°C, the pressure is 0.11MPa, and the high temperature The sterilization time is longer than 30 minutes;
（3）发酵罐在连通三通阀之前罐内进行高温灭菌，发酵罐的进料口通过排热蒸汽来保持灭菌；所述高温灭菌的温度为125℃，压力为0.13MPa，高温灭菌时间大于30分钟即可；(3) The fermenter is subjected to high-temperature sterilization in the tank before the three-way valve is connected, and the feed port of the fermenter is kept sterilized by exhausting steam; the temperature of the high-temperature sterilization is 125°C, the pressure is 0.13MPa, and the high temperature The sterilization time is longer than 30 minutes;
（4）连通目的为将1个或2个的发酵罐与三通阀的硅胶软管接口连通，本实施例连接2个发酵罐，种子罐则与三通阀未连接发酵罐的一个硅胶软管接口连通；(4) The purpose of connection is to connect one or two fermentation tanks with the silicone hose interface of the three-way valve. pipe connection;
（5）硅胶软管与种子罐连通时，先将种子罐的出料口与待连接的硅胶软管接触，用火焰外焰烧烤种子罐的出料口和硅胶软管的连接处，然后关掉种子罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现种子罐与三通阀的无菌对接。(5) When the silicone hose is connected to the seed tank, first contact the outlet of the seed tank with the silicone hose to be connected, and use the flame to grill the connection between the outlet of the seed tank and the silicone hose, and then close the Remove the hot steam from the seed tank, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the sterile docking of the seed tank and the three-way valve.
（6）硅胶软管与发酵罐连通时，先将发酵罐的进料口与待连接的硅胶软管接触，用火焰外焰烧烤发酵罐的进料口和硅胶软管的连接处，然后关掉发酵罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现发酵罐与三通阀的无菌对接。(6) When the silicone hose is connected to the fermenter, first contact the feed port of the fermenter with the silicone hose to be connected, grill the connection between the feed port of the fermenter and the silicone hose with an external flame, and then close the Remove the hot steam from the fermenter, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the aseptic connection between the fermenter and the three-way valve.
连接后，所述三通阀的进口与种子罐连通，所述三通阀的两个出口与分别2个发酵罐连通。由此实现液体菌种一对多管道输送的无菌输送。After connection, the inlet of the three-way valve communicates with the seed tank, and the two outlets of the three-way valve communicate with two fermentation tanks respectively. Thus, the aseptic delivery of one-to-many pipeline delivery of liquid bacteria is realized.
实施例2Example 2
本发明实施例包括以下步骤，本实施例中n通阀为五通阀。The embodiment of the present invention includes the following steps. In this embodiment, the n-way valve is a five-way valve.
（1）将五通阀的所有端口处连接有硅胶软管，所述硅胶软管的端口处均用棉花包裹密封，然后一并放置在高压灭菌锅内保存，所述高温灭菌的温度为123℃，压力为0.11MPa；所述硅胶软管内径为1cm。(1) Connect all the ports of the five-way valve with silicone hoses. The ports of the silicone hoses are wrapped and sealed with cotton, and then placed in an autoclave for storage. The temperature of the high-temperature sterilization The temperature is 123° C., the pressure is 0.11 MPa; the inner diameter of the silicone hose is 1 cm.
（2）种子罐在连通五通阀之前罐内进行高温灭菌，种子罐的出料口通过排热蒸汽来保持灭菌；所述高温灭菌的温度为122℃，压力为0.15MPa；(2) Before the seed tank is connected to the five-way valve, high-temperature sterilization is carried out in the tank, and the discharge port of the seed tank is kept sterilized by exhausting steam; the temperature of the high-temperature sterilization is 122°C and the pressure is 0.15MPa;
（3）发酵罐在连通五通阀之前罐内进行高温灭菌，发酵罐的进料口通过排热蒸汽来保持灭菌；所述高温灭菌的温度为122℃，压力为0.11MPa；(3) The fermenter is subjected to high-temperature sterilization in the tank before the five-way valve is connected, and the feed port of the fermenter is kept sterilized by exhausting steam; the temperature of the high-temperature sterilization is 122°C and the pressure is 0.11MPa;
（4）连通目的为将1个、2个、3个或4个的发酵罐与五通阀的硅胶软管接口连通，本实施例连接3个发酵罐，种子罐则与五通阀未连接发酵罐的一个硅胶软管接口连通；(4) The purpose of connection is to connect 1, 2, 3 or 4 fermentation tanks with the silicone hose interface of the five-way valve. In this embodiment, three fermentation tanks are connected, and the seed tank is not connected to the five-way valve. A silicone hose port of the fermenter is connected;
（5）硅胶软管与种子罐连通时，先将种子罐的出料口与待连接的硅胶软管接触，用火焰外焰烧烤种子罐的出料口和硅胶软管的连接处，然后关掉种子罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现种子罐与五通阀的无菌对接。(5) When the silicone hose is connected to the seed tank, first contact the outlet of the seed tank with the silicone hose to be connected, and use the flame to grill the connection between the outlet of the seed tank and the silicone hose, and then close the Remove the hot steam from the seed tank, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the sterile docking of the seed tank and the five-way valve.
（6）硅胶软管与发酵罐连通时，先将发酵罐的进料口与待连接的硅胶软管接触，用火焰外焰烧烤发酵罐的进料口和硅胶软管的连接处，然后关掉发酵罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现发酵罐与五通阀的无菌对接。(6) When the silicone hose is connected to the fermenter, first contact the feed port of the fermenter with the silicone hose to be connected, grill the connection between the feed port of the fermenter and the silicone hose with an external flame, and then close the Remove the hot steam from the fermenter, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the aseptic connection between the fermenter and the five-way valve.
连接后，所述五通阀的进口与种子罐连通，所述五通阀的出口与分别3个发酵罐连通。由此实现液体菌种一对多管道输送的无菌输送。After connection, the inlet of the five-way valve communicates with the seed tank, and the outlet of the five-way valve communicates with three fermentation tanks respectively. Thus, the aseptic delivery of one-to-many pipeline delivery of liquid bacteria is realized.
实施例3Example 3
本发明实施例包括以下步骤，本实施例中n通阀为五通阀。The embodiment of the present invention includes the following steps. In this embodiment, the n-way valve is a five-way valve.
（1）将五通阀的所有端口处连接有硅胶软管，所述硅胶软管的端口处均用棉花包裹密封，然后一并放置在高压灭菌锅内保存，所述高温灭菌的温度为125℃，压力为0.12MPa；所述硅胶软管内径为1cm。(1) Connect all the ports of the five-way valve with silicone hoses. The ports of the silicone hoses are wrapped and sealed with cotton, and then placed in an autoclave for storage. The temperature of the high-temperature sterilization The temperature is 125°C, the pressure is 0.12MPa; the inner diameter of the silicone hose is 1cm.
（2）种子罐在连通五通阀之前罐内进行高温灭菌，种子罐的出料口通过排热蒸汽来保持灭菌；所述高温灭菌的温度为125℃，压力为0.13MPa；(2) Before the seed tank is connected to the five-way valve, high-temperature sterilization is carried out in the tank, and the discharge port of the seed tank is kept sterilized by exhausting steam; the temperature of the high-temperature sterilization is 125°C and the pressure is 0.13MPa;
（3）发酵罐在连通五通阀之前罐内进行高温灭菌，发酵罐的进料口通过排热蒸汽来保持灭菌；所述高温灭菌的温度为124℃，压力为0.15MPa；(3) Before the fermenter is connected to the five-way valve, high-temperature sterilization is carried out in the tank, and the feed port of the fermenter is kept sterilized by exhausting steam; the temperature of the high-temperature sterilization is 124°C and the pressure is 0.15MPa;
（4）连通目的为将1个、2个、3个或4个的发酵罐与五通阀的硅胶软管接口连通，本实施例连接4个发酵罐，种子罐则与五通阀未连接发酵罐的一个硅胶软管接口连通；(4) The purpose of connection is to connect 1, 2, 3 or 4 fermentation tanks with the silicone hose interface of the five-way valve. In this embodiment, four fermentation tanks are connected, and the seed tank is not connected to the five-way valve. A silicone hose port of the fermenter is connected;
（5）硅胶软管与种子罐连通时，先将种子罐的出料口与待连接的硅胶软管接触，用火焰外焰烧烤种子罐的出料口和硅胶软管的连接处，然后关掉种子罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现种子罐与五通阀的无菌对接。(5) When the silicone hose is connected to the seed tank, first contact the outlet of the seed tank with the silicone hose to be connected, and use the flame to grill the connection between the outlet of the seed tank and the silicone hose, and then close the Remove the hot steam from the seed tank, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the sterile docking of the seed tank and the five-way valve.
（6）硅胶软管与发酵罐连通时，先将发酵罐的进料口与待连接的硅胶软管接触，用火焰外焰烧烤发酵罐的进料口和硅胶软管的连接处，然后关掉发酵罐的热蒸汽，在火焰的保护下移除包裹在该硅胶软管的棉花，实现发酵罐与五通阀的无菌对接。(6) When the silicone hose is connected to the fermenter, first contact the feed port of the fermenter with the silicone hose to be connected, grill the connection between the feed port of the fermenter and the silicone hose with an external flame, and then close the Remove the hot steam from the fermenter, remove the cotton wrapped in the silicone hose under the protection of the flame, and realize the aseptic connection between the fermenter and the five-way valve.
连接后，所述五通阀的进口与种子罐连通，所述五通阀的出口与分别4个发酵罐连通。由此实现液体菌种一对多管道输送的无菌输送。After connection, the inlet of the five-way valve communicates with the seed tank, and the outlet of the five-way valve communicates with four fermentation tanks respectively. Thus, the aseptic delivery of one-to-many pipeline delivery of liquid bacteria is realized.
经过测试，使用本发明输送方法，在种子罐与发酵罐之间进行菌种输送，菌种的污染率大大降低，污染率仅为1%-0.5%。After testing, using the transportation method of the present invention to transport bacteria between the seed tank and the fermentation tank, the pollution rate of the bacteria is greatly reduced, and the pollution rate is only 1%-0.5%.
本发明方法生产成本大大降低，便于农户大规模推广，操作简便，适用范围广。The method of the invention greatly reduces the production cost, is convenient for farmers to popularize on a large scale, is easy to operate, and has a wide application range.
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明专利的精神或范围的情况下，在其他实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。The above description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the patented invention. Therefore, the present invention will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
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| WO2002032816A1 (en) * | 2000-10-13 | 2002-04-25 | Inbionet Corporation | Apparatus and process for bioconverting high-concentrated organic waste |
| CN105505744A (en) * | 2016-01-07 | 2016-04-20 | 盐城市雨露农业科技有限公司 | Liquid strain seed tank and using method thereof |
| CN205902434U (en) * | 2016-08-19 | 2017-01-25 | 四川宝生药业发展有限公司 | Deep culture apparatus of tubular |
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|---|---|---|---|---|
| CN2075203U (en) * | 1990-09-07 | 1991-04-17 | 梁希珍 | Liquid injecting device for biological ferment |
| WO2002032816A1 (en) * | 2000-10-13 | 2002-04-25 | Inbionet Corporation | Apparatus and process for bioconverting high-concentrated organic waste |
| CN105505744A (en) * | 2016-01-07 | 2016-04-20 | 盐城市雨露农业科技有限公司 | Liquid strain seed tank and using method thereof |
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Application publication date: 20180313 |