CN205902434U - Deep culture apparatus of tubular - Google Patents
Deep culture apparatus of tubular Download PDFInfo
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- CN205902434U CN205902434U CN201620904325.7U CN201620904325U CN205902434U CN 205902434 U CN205902434 U CN 205902434U CN 201620904325 U CN201620904325 U CN 201620904325U CN 205902434 U CN205902434 U CN 205902434U
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- 238000000855 fermentation Methods 0.000 claims abstract description 109
- 230000004151 fermentation Effects 0.000 claims abstract description 109
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 229920000742 Cotton Polymers 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 7
- 239000002994 raw material Substances 0.000 description 17
- 238000011534 incubation Methods 0.000 description 6
- 238000009434 installation Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model discloses a deep culture apparatus of tubular, including multiunit fermentation unit, electromagnetic pump, ball formula filter, multiunit fermentation unit comprises at least a set of fermented unit, and a set of fermented unit includes fermentation cylinder, first three -way valve, and a play liquid end of first three -way valve is connected with the fermentation cylinder, and the ferment unit connection is compiled and published with next to another liquid end of first three -way valve, and the electromagnetic pump passes through the connecting pipe to be connected with ball formula filter, and ball formula filter is connected with the feed liquor end of first three -way valve. The utility model discloses simple structure, the operation of being convenient for, whole operation process is airtight in succession, does not have to introduce and pollutes, realizes aseptic technique.
Description
Technical field
This utility model is related to device for culturing liquid strain technical field and in particular to a kind of tubular type depth culture device.
Background technology
The method of the commonly used multistage solid spawn amplification culture of acquisition of liquid spawn at present, but this cultural method behaviour
Make loaded down with trivial details, high labor intensive, incubation time length, cell age inconsistent, production cost is expensive.It is readily incorporated pollution in operating simultaneously,
The liquid spawn amount that final impact produces.
Based on this, study and develop a kind of tubular type depth culture device of design.
Content of the invention
Technical problem to be solved in the utility model is existing bacterial strain culture device complex structure, complex operation, easily
Introduce operational pollution.This utility model purpose is to provide a kind of tubular type depth culture device, using multigroup fermentation unit, every
On the fermentation tank of group fermentation unit, at least one feed tube is set, the airtight continuous culture of strain, when solving strain deep drainpipe
Between long, introduce pollution etc. technical problem.
Of the present utility model it is achieved through the following technical solutions:
A kind of tubular type depth culture device, including multigroup fermentation unit, electromagnetic pump, ball filter, multigroup fermentation unit
It is made up of least one set fermentation unit, one group of fermentation unit includes fermentation tank, the first three-way valve, one of the first three-way valve goes out liquid
End is connected with fermentation tank, and another liquid outlet of the first three-way valve is organized installation for fermenting with next and is connected, electromagnetic pump by connecting tube and
Ball filter connects, and ball filter is connected with the liquid feeding end of the first three-way valve.
Inventor's the technical program through research design, designs electromagnetic pump, conveys strain in the presence of electromagnetic pump
To the fermentation tank of fermentation unit, start deep drainpipe.Before entering fermentation tank, setting ball filter filters to strain raw material
Operation, removes impurity contained therein.
It is equipped with fermentation tank in every group of fermentation unit, the first three-way valve, strain raw material, after the filtration of ball filter, leads to
Cross the first three-way valve of setting in the connecting tube of place, the setting of the first three-way valve, the flow direction of controllable strain raw material liquid, stream
Amount, can simultaneously enter the fermentation tank of one group of fermentation unit it is also possible to simultaneously enter the fermentation tank of other multigroup fermentation unit.And it is whole
Individual process is airtight, less with contacting external air, prevents from bringing pollution, thus avoiding manual operation to pollute.
Preferably, it is equipped with feed tube in the fermentation tank in described every group of fermentation unit, the body of feed tube is provided with filtration
Device.On the basis of an existing connecting tube extends in the fermentation tank of every group of fermentation unit, the setting of feed tube, make this technology
Tubular type depth culture device described in scheme forms double hose culture apparatuses.The effect of setting feed tube is can be easy to fermentation tank
Need the strain raw material cultivated.Filter is arranged on the body of feed tube, is to sending out in every group of fermentation unit of entrance
The strain raw material of fermentation tank carries out filtering and impurity removing sterilization.
Preferably, it is equipped with threeway feed tube, on the body of threeway feed tube in the fermentation tank in described one group of fermentation unit
It is provided with the second three-way valve.
On the basis of existing two connecting tubes all extend in the fermentation tank of every group of fermentation unit, the setting of threeway feed tube
Put, make the tubular type depth culture device described in the technical program form three-tube type culture apparatuses.The effect of setting threeway feed tube,
Can be easy to add strain raw material toward in the fermentation tank in every group of fermentation unit.
Threeway feed tube is One In and Two Out, and strain raw material is delivered to fermentation tank by a liquid feeding end, liquid outlet with every
The fermentation tank of group fermentation unit connects, and another liquid outlet is connected with the threeway feed tube in next group fermentation unit, and every group
Threeway feed tube between fermentation unit is structure as a whole.I.e. a liquid outlet of first group of threeway feed tube enters first group
In the fermentation tank of fermentation unit, another liquid outlet of first group of threeway feed tube and the threeway feed liquor of second group of fermentation unit
Pipe connects, and the liquid feeding end simultaneously as threeway feed tube in second group of fermentation unit.
Wherein, the effect of setting the second three-way valve is to control the flow direction of strain raw material, flow in threeway feed tube, and controls
Second three-way valve state, can convey raw material to be cultivated in the fermentation tank of every group of fermentation unit simultaneously, carry out fermentation training simultaneously
Support, thus shortening incubation time.
Preferably, it is connected with each other between the threeway feed tube of described fermentation unit.Knot is connected as one between threeway feed tube
Structure, then entered the strain raw material in the fermentation tank of every group of fermentation unit or strain, all conveyed by pipeline closed, and is all from same
One liquid feeding end.During this, can reduce and the contacting external air time, it is to avoid introduce operational pollution to culture apparatuses, real
Existing sterile working.
Preferably, the feed tube of described every group of fermentation unit is equipped with switch.Control water influent pipeline can be arranged on feed tube
The switch connecting with the fermentation tank in every group of fermentation unit.After opening switch, strain raw material or strain are removed by filter
Bacterium remove impurity is processed.
Preferably, the opening of the fermentation tank of described every group of fermentation unit is equipped with sealing-plug.The setting of sealing-plug increases fermentation
The seal of tank.
Preferably, described sealing-plug is provided with the cotton mass in gap between plug for seal plug and the opening of fermentation tank.Cotton mass
Be disposed to preferably strengthen the seal of fermentation tank, prevent introduced contaminants from entering the airtight training of its internal influence strain
Support, realize sterile working, there is no operational pollution.Meanwhile, short tube can be set at opening, and short tube passes through sealing-plug, stretches to cotton
In flower group, it is easy to give vent to anger.
Wherein, described every group represent numerous in one group, this form of presentation be this area common knowledge.
This utility model compared with prior art, has such advantages as and beneficial effect:
(1) this utility model passes through to arrange multigroup fermentation unit, and every group of fermentation unit all includes fermentation tank, the first threeway
Valve, forms single hose depth culture device, can realize same by controlling the first three-way valve, thus controlling the flow direction of strain, flow
When carry out fermentation culture in different group installation for fermentings, realize airtight continuous culture, contactless with the external world, realize no pollution, and
Ferment, incubation time greatly shortens simultaneously.
(2) in this utility model, the fermentation tank in every group of installation for fermenting is respectively provided with feed tube, forms double hose deep drainpipe
Device is it is adaptable to need in culture apparatuses to add the situation of at least one strain raw material.
(3) it is equipped with threeway feed tube in the fermentation tank of every group of fermentation unit in this utility model, be equally applicable to train
Need to add the situation of at least one strain raw material in foster device, threeway feed tube is provided with the second threeway injection valve, to every
In group fermentation tank, the process of addition strain raw material or strain is all airtight continuous, pollution-free introducing, and ferments simultaneously in each fermentation tank
In carry out, shorten incubation time, achievable large-scale production.
Brief description
Accompanying drawing described herein is used for providing this utility model embodiment is further understood, and constitutes the one of the application
Part, does not constitute the restriction to this utility model embodiment.In the accompanying drawings:
Fig. 1 is this utility model structural representation；
Fig. 2 is this utility model twin-tube type structure schematic diagram；
Fig. 3 is this utility model three-tube type structural representation；
Labelling and corresponding parts title in accompanying drawing:
1- electromagnetic pump, 2- ball filter, 3- fermentation tank, 4- sealing-plug, 5- first three-way valve, 6- feed tube, 7- filters
Device, 8- second three-way valve, 9- threeway feed tube.
Specific embodiment
For making the purpose of this utility model, technical scheme and advantage become more apparent, with reference to embodiment and accompanying drawing,
The utility model is described in further detail, and exemplary embodiment of the present utility model and its explanation are only used for explaining this
Utility model, is not intended as to restriction of the present utility model.
Embodiment 1:
As shown in Figure 1, Figure 2, Figure 3 shows, a kind of tubular type depth culture device, including multigroup fermentation unit, electromagnetic pump 1, ball
Filter 2, multigroup fermentation unit is made up of least one set fermentation unit, and one group of fermentation unit includes fermentation tank 3, the first three-way valve
5, a liquid outlet of the first three-way valve 5 is connected with fermentation tank 3, and another liquid outlet of the first three-way valve 5 is fermented with next group
Device connects, and electromagnetic pump 1 is connected with ball filter 2 by connecting tube, the liquid feeding end of ball filter 2 and the first three-way valve 5
Connect.
Wherein, it is equipped with feed tube 6 in the fermentation tank 3 in described one group of fermentation unit, the body of feed tube 6 was provided with
Filter 7.
Wherein, it is equipped with threeway feed tube 9, the body of threeway feed tube 9 in the fermentation tank 3 in described one group of fermentation unit
It is provided with the second three-way valve 8.
Wherein, it is connected with each other between the threeway feed tube 9 of described fermentation unit.
Wherein, the feed tube 6 of described one group of fermentation unit is equipped with switch.
Wherein, the opening of the fermentation tank 3 of described one group of fermentation unit is equipped with sealing-plug 4.
Wherein, described sealing-plug 4 is provided with the cotton mass in gap between plug for seal plug 4 and the opening of fermentation tank 3.
In embodiment 1, multigroup fermentation unit is set, every group of fermentation unit all includes fermentation tank 3, the first three-way valve 5, is formed
Single hose depth culture device, can realize simultaneously not by controlling the first three-way valve 5, thus controlling the flow direction of strain, flow
Carry out fermentation culture with group fermentation unit, airtight continuous culture is realized in whole culture, contactless with the external world, realizes no pollution,
And ferment, shorten incubation time. simultaneously.
It is equipped with feed tube 6 in embodiment 1 in the fermentation tank 3 of every group of installation for fermenting, form double hose deep drainpipe dress
Put, be applicable to the situation needing in culture apparatuses to add at least one strain raw material.
It is equipped with threeway feed tube 9 in the fermentation tank 3 of every group of fermentation unit in embodiment 1, be equally applicable to culture apparatuses
Middle need to add the situation of at least one strain raw material, threeway feed tube 9 is provided with the second three-way valve, to every group of fermentation tank 3
The process of middle addition strain raw material or strain is all airtight continuous, pollution-free introducing, and fermentation is carried out in each fermentation tank 3 simultaneously,
Shorten incubation time, realize large-scale production.
Above-described specific embodiment, is entered to the purpose of this utility model, technical scheme and beneficial effect
One step describes in detail, be should be understood that and the foregoing is only specific embodiment of the present utility model, is not used to limit
Fixed protection domain of the present utility model, all any modifications within spirit of the present utility model and principle, made, equivalent replaces
Change, improve, should be included within protection domain of the present utility model.
Claims (7)
1. a kind of tubular type depth culture device is it is characterised in that include multigroup fermentation unit, electromagnetic pump (1), ball filter
(2), multigroup fermentation unit is made up of least one set fermentation unit, and one group of fermentation unit includes fermentation tank (3), the first three-way valve
(5), a liquid outlet of the first three-way valve (5) is connected with fermentation tank (3), another liquid outlet of the first three-way valve (5) and next
Group fermentation unit connects, and electromagnetic pump (1) is connected with ball filter (2) by connecting tube, ball filter (2) and the first threeway
The liquid feeding end of valve (5) connects.
2. a kind of tubular type depth culture device according to claim 1 it is characterised in that: in described every group of fermentation unit
It is equipped with feed tube (6), the body of feed tube (6) is provided with filter (7) in fermentation tank (3).
3. a kind of tubular type depth culture device according to claim 2 it is characterised in that: in described every group of fermentation unit
It is equipped with threeway feed tube (9), the body of threeway feed tube (9) is provided with the second three-way valve (8) in fermentation tank (3).
4. a kind of tubular type depth culture device according to claim 3 it is characterised in that: the threeway of described fermentation unit is entered
Liquid pipe is connected with each other between (9).
5. a kind of tubular type depth culture device according to claim 4 it is characterised in that: the entering of described every group of fermentation unit
Liquid pipe is equipped with switch on (6).
6. a kind of tubular type depth culture device according to any one of claim 1 to 5 it is characterised in that: send out for described every group
The opening of the fermentation tank (3) of ferment unit is equipped with sealing-plug (4).
7. a kind of tubular type depth culture device according to claim 6 it is characterised in that: described sealing-plug (4) is provided with
The cotton mass in gap between the opening of plug for seal plug and fermentation tank (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620904325.7U CN205902434U (en) | 2016-08-19 | 2016-08-19 | Deep culture apparatus of tubular |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620904325.7U CN205902434U (en) | 2016-08-19 | 2016-08-19 | Deep culture apparatus of tubular |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205902434U true CN205902434U (en) | 2017-01-25 |
Family
ID=57800860
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620904325.7U Active CN205902434U (en) | 2016-08-19 | 2016-08-19 | Deep culture apparatus of tubular |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205902434U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794227A (en) * | 2017-10-26 | 2018-03-13 | 翔天农业开发集团股份有限公司 | A kind of sterile carrying method of the one-to-many pipeline of liquid spawn |
-
2016
- 2016-08-19 CN CN201620904325.7U patent/CN205902434U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794227A (en) * | 2017-10-26 | 2018-03-13 | 翔天农业开发集团股份有限公司 | A kind of sterile carrying method of the one-to-many pipeline of liquid spawn |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |